WFS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	WFS1
Entrez Gene ID	100298456
Full Name	wolframin ER transmembrane glycoprotein
General protein information	Preferred Names wolframin ER transmembrane glycoprotein Names wolframin Wolfram syndrome 1 (wolframin) Wolfram syndrome 1 protein homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003582346.4	XP_003582394.1	wolframin
XM_015471710.2	XP_015327196.1	wolframin
XM_015471710.1	XP_015327196.1	wolframin
XM_015463832.1	XP_015319318.1	wolframin
XM_002688400.4	XP_002688446.1	wolframin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WFS1	XP_003310288.1
Danio rerio (zebrafish)	wfs1b	XP_005157280.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004409	XP_313695.5
Homo sapiens (human)	WFS1	NP_001139325.1
	WFS1	XP_001092811.1
Xenopus tropicalis (tropical clawed frog)	wfs1	NP_001106459.1
Canis lupus familiaris (dog)	WFS1	XP_539234.4
Bos taurus (cattle)	WFS1	XP_002688446.1
Gallus gallus (chicken)	WFS1	XP_420803.2
Drosophila melanogaster (fruit fly)	wfs1	NP_001189267.1
Mus musculus (house mouse)	Wfs1	NP_035846.1
Rattus norvegicus (Norway rat)	Wfs1	NP_114011.1

Gene Ontology

The following WFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa04894	XM_003582346.4 Latest version!	Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa04894	XM_015471710.2 Latest version!	Bos taurus wolframin ER transmembrane glycoprotein (WFS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa04894	XM_015463832.1 Latest version!	Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa04894	XM_002688400.4 Latest version!	Bos taurus wolframin ER transmembrane glycoprotein (WFS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa04894	XM_015471710.1	Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WFS1 ( XM_003582346.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582394.1 Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa04894
Clone ID Related Accession (Same CDS sequence)	XM_003582346.4 , XM_015471710.2 , XM_015471710.1 , XM_015463832.1 , XM_002688400.4
Accession Version	XM_003582346.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644958.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582346.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582394.1
CDS	166..2832
Translation	MASSASPPSSSGPQLPPPPQSQARSRLNATTSVEQEFSGAPPAPGPQVGPGSDVRDPAAPAMPQASRARSRERMDGTGPAKGDVDTPFEEVLEKAKAGDPKAQTEVGKHYLQLAGDGDEELNNCTAVDWLTLAAKQGRREAVKLLRRCLADRRGITSENEQEVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGVQPGPVPKSLQKQRRVLERLVSSESKSYIALDDFVEITKKYAKGIIPTDLFLQDDDDDEVAGKSPEDLPLRLKVVKYPLHVIMELKEYLIDLASRAGVHWLSTLVPTHHINALVFFFIISNLTIDFFAFFVPLVVFYLSFISMVICTLKVFQDSKAWESFRALTSLLLRFEPNLDVEQAEGNFGWNHLEPYVHFLLSVFFVIISFPIASKDCMPCSELAVVSVFFTATSYMSLSTSAEPYTRRALVTEVAAGLLSLLPTMPVNWRYLKLLGQTFFTVPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLVRASVGYFLFLFALPILVFALALMGVVQLARWFVSLELTKIAVTLVLCSVPLLVRWWTKANFSVVGMVKSLTRSSIVKLILVWLTAIVLFCWFYLYRSEGMKVYNSTLTWPQYGFLCGPRAWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAVHMLPLFLGDWLRCLYGEAYPSCSPGNVSTAEEELCRLKLLAKHPCHMKRFDRYKFEITVGMPYSGANGSRGPEEDDVTKDIVLRASSEFKDVLLHLRQGSVIEFSTVLEGRLGSKWPVFELKAISCLNCMAHLVPARRHVKVERDWRSAVHGAVKFAFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_003582346.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582346.4

ORF Insert Sequence:

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_015471710.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327196.1 Bos taurus wolframin ER transmembrane glycoprotein (WFS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa04894
Clone ID Related Accession (Same CDS sequence)	XM_003582346.4 , XM_015471710.2 , XM_015471710.1 , XM_015463832.1 , XM_002688400.4
Accession Version	XM_015471710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327196.1
CDS	23..2689
Translation	MASSASPPSSSGPQLPPPPQSQARSRLNATTSVEQEFSGAPPAPGPQVGPGSDVRDPAAPAMPQASRARSRERMDGTGPAKGDVDTPFEEVLEKAKAGDPKAQTEVGKHYLQLAGDGDEELNNCTAVDWLTLAAKQGRREAVKLLRRCLADRRGITSENEQEVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGVQPGPVPKSLQKQRRVLERLVSSESKSYIALDDFVEITKKYAKGIIPTDLFLQDDDDDEVAGKSPEDLPLRLKVVKYPLHVIMELKEYLIDLASRAGVHWLSTLVPTHHINALVFFFIISNLTIDFFAFFVPLVVFYLSFISMVICTLKVFQDSKAWESFRALTSLLLRFEPNLDVEQAEGNFGWNHLEPYVHFLLSVFFVIISFPIASKDCMPCSELAVVSVFFTATSYMSLSTSAEPYTRRALVTEVAAGLLSLLPTMPVNWRYLKLLGQTFFTVPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLVRASVGYFLFLFALPILVFALALMGVVQLARWFVSLELTKIAVTLVLCSVPLLVRWWTKANFSVVGMVKSLTRSSIVKLILVWLTAIVLFCWFYLYRSEGMKVYNSTLTWPQYGFLCGPRAWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAVHMLPLFLGDWLRCLYGEAYPSCSPGNVSTAEEELCRLKLLAKHPCHMKRFDRYKFEITVGMPYSGANGSRGPEEDDVTKDIVLRASSEFKDVLLHLRQGSVIEFSTVLEGRLGSKWPVFELKAISCLNCMAHLVPARRHVKVERDWRSAVHGAVKFAFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_015471710.2
Organism	Bos taurus(cattle)
Definition	Bos taurus wolframin ER transmembrane glycoprotein (WFS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471710.2

ORF Insert Sequence:

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_015471710.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327196.1 Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa04894
Clone ID Related Accession (Same CDS sequence)	XM_003582346.4 , XM_015471710.2 , XM_015471710.1 , XM_015463832.1 , XM_002688400.4
Accession Version	XM_015471710.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103983.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327196.1
CDS	156..2822
Translation	MASSASPPSSSGPQLPPPPQSQARSRLNATTSVEQEFSGAPPAPGPQVGPGSDVRDPAAPAMPQASRARSRERMDGTGPAKGDVDTPFEEVLEKAKAGDPKAQTEVGKHYLQLAGDGDEELNNCTAVDWLTLAAKQGRREAVKLLRRCLADRRGITSENEQEVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGVQPGPVPKSLQKQRRVLERLVSSESKSYIALDDFVEITKKYAKGIIPTDLFLQDDDDDEVAGKSPEDLPLRLKVVKYPLHVIMELKEYLIDLASRAGVHWLSTLVPTHHINALVFFFIISNLTIDFFAFFVPLVVFYLSFISMVICTLKVFQDSKAWESFRALTSLLLRFEPNLDVEQAEGNFGWNHLEPYVHFLLSVFFVIISFPIASKDCMPCSELAVVSVFFTATSYMSLSTSAEPYTRRALVTEVAAGLLSLLPTMPVNWRYLKLLGQTFFTVPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLVRASVGYFLFLFALPILVFALALMGVVQLARWFVSLELTKIAVTLVLCSVPLLVRWWTKANFSVVGMVKSLTRSSIVKLILVWLTAIVLFCWFYLYRSEGMKVYNSTLTWPQYGFLCGPRAWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAVHMLPLFLGDWLRCLYGEAYPSCSPGNVSTAEEELCRLKLLAKHPCHMKRFDRYKFEITVGMPYSGANGSRGPEEDDVTKDIVLRASSEFKDVLLHLRQGSVIEFSTVLEGRLGSKWPVFELKAISCLNCMAHLVPARRHVKVERDWRSAVHGAVKFAFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_015471710.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471710.1

ORF Insert Sequence:

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_015463832.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319318.1 Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa04894
Clone ID Related Accession (Same CDS sequence)	XM_003582346.4 , XM_015471710.2 , XM_015471710.1 , XM_015463832.1 , XM_002688400.4
Accession Version	XM_015463832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644958.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319318.1
CDS	156..2822
Translation	MASSASPPSSSGPQLPPPPQSQARSRLNATTSVEQEFSGAPPAPGPQVGPGSDVRDPAAPAMPQASRARSRERMDGTGPAKGDVDTPFEEVLEKAKAGDPKAQTEVGKHYLQLAGDGDEELNNCTAVDWLTLAAKQGRREAVKLLRRCLADRRGITSENEQEVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGVQPGPVPKSLQKQRRVLERLVSSESKSYIALDDFVEITKKYAKGIIPTDLFLQDDDDDEVAGKSPEDLPLRLKVVKYPLHVIMELKEYLIDLASRAGVHWLSTLVPTHHINALVFFFIISNLTIDFFAFFVPLVVFYLSFISMVICTLKVFQDSKAWESFRALTSLLLRFEPNLDVEQAEGNFGWNHLEPYVHFLLSVFFVIISFPIASKDCMPCSELAVVSVFFTATSYMSLSTSAEPYTRRALVTEVAAGLLSLLPTMPVNWRYLKLLGQTFFTVPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLVRASVGYFLFLFALPILVFALALMGVVQLARWFVSLELTKIAVTLVLCSVPLLVRWWTKANFSVVGMVKSLTRSSIVKLILVWLTAIVLFCWFYLYRSEGMKVYNSTLTWPQYGFLCGPRAWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAVHMLPLFLGDWLRCLYGEAYPSCSPGNVSTAEEELCRLKLLAKHPCHMKRFDRYKFEITVGMPYSGANGSRGPEEDDVTKDIVLRASSEFKDVLLHLRQGSVIEFSTVLEGRLGSKWPVFELKAISCLNCMAHLVPARRHVKVERDWRSAVHGAVKFAFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_015463832.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463832.1

ORF Insert Sequence:

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_002688400.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002688446.1 Bos taurus wolframin ER transmembrane glycoprotein (WFS1), transcript variant X1, mRNA.

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CloneID	OBa04894
Clone ID Related Accession (Same CDS sequence)	XM_003582346.4 , XM_015471710.2 , XM_015471710.1 , XM_015463832.1 , XM_002688400.4
Accession Version	XM_002688400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002688446.1
CDS	166..2832
Translation	MASSASPPSSSGPQLPPPPQSQARSRLNATTSVEQEFSGAPPAPGPQVGPGSDVRDPAAPAMPQASRARSRERMDGTGPAKGDVDTPFEEVLEKAKAGDPKAQTEVGKHYLQLAGDGDEELNNCTAVDWLTLAAKQGRREAVKLLRRCLADRRGITSENEQEVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGVQPGPVPKSLQKQRRVLERLVSSESKSYIALDDFVEITKKYAKGIIPTDLFLQDDDDDEVAGKSPEDLPLRLKVVKYPLHVIMELKEYLIDLASRAGVHWLSTLVPTHHINALVFFFIISNLTIDFFAFFVPLVVFYLSFISMVICTLKVFQDSKAWESFRALTSLLLRFEPNLDVEQAEGNFGWNHLEPYVHFLLSVFFVIISFPIASKDCMPCSELAVVSVFFTATSYMSLSTSAEPYTRRALVTEVAAGLLSLLPTMPVNWRYLKLLGQTFFTVPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLVRASVGYFLFLFALPILVFALALMGVVQLARWFVSLELTKIAVTLVLCSVPLLVRWWTKANFSVVGMVKSLTRSSIVKLILVWLTAIVLFCWFYLYRSEGMKVYNSTLTWPQYGFLCGPRAWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAVHMLPLFLGDWLRCLYGEAYPSCSPGNVSTAEEELCRLKLLAKHPCHMKRFDRYKFEITVGMPYSGANGSRGPEEDDVTKDIVLRASSEFKDVLLHLRQGSVIEFSTVLEGRLGSKWPVFELKAISCLNCMAHLVPARRHVKVERDWRSAVHGAVKFAFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_002688400.4
Organism	Bos taurus(cattle)
Definition	Bos taurus wolframin ER transmembrane glycoprotein (WFS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688400.4

ORF Insert Sequence:

ATGGCTTCCA GCGCCTCTCC TCCGAGCTCC TCTGGCCCCC AGCTTCCGCC GCCACCGCAG 
TCCCAAGCCC GCTCACGGCT CAACGCCACC ACCTCGGTGG AGCAGGAGTT CAGTGGGGCG 
CCCCCCGCTC CTGGACCCCA AGTTGGACCT GGATCAGATG TGAGAGACCC AGCTGCCCCG 
GCCATGCCCC AGGCCTCGCG GGCCAGGAGC CGAGAGAGGA TGGATGGAAC AGGACCTGCA 
AAGGGAGATG TGGACACGCC CTTTGAGGAA GTCCTGGAGA AGGCCAAGGC TGGGGACCCC 
AAGGCACAGA CGGAGGTGGG GAAGCATTAC CTGCAGCTGG CCGGCGACGG GGATGAAGAG 
CTCAACAACT GCACCGCCGT GGACTGGCTG ACCCTCGCCG CCAAGCAGGG CCGGCGCGAG 
GCCGTCAAGC TTCTCCGCCG ATGCCTGGCG GACAGGAGAG GCATCACCTC CGAGAACGAG 
CAGGAGGTAA GGCAGCTGTC CTCTGAGACC GACCTGGAGC GGGCTGTGCG CAAGGCGGCC 
CTCGTCATGT ACTGGAAGCT CAACCCCAAG AAGAAAAAGC AGGTAGCCGT GGCGGAGCTG 
CTGGAGAACG TGGGGCAGGT CAACGAGCAC GATGGCGGGG TGCAGCCCGG GCCCGTCCCC 
AAGTCCCTGC AGAAGCAGAG GCGGGTGCTG GAACGCTTGG TCAGCAGTGA ATCCAAGAGC 
TACATAGCCC TGGATGACTT TGTGGAGATC ACCAAGAAGT ACGCCAAGGG CATCATCCCC 
ACCGACCTGT TCCTGCAGGA CGACGATGAT GACGAGGTGG CGGGCAAGAG TCCCGAGGAC 
CTACCCCTGC GCCTGAAGGT GGTCAAGTAC CCCTTGCACG TCATCATGGA GCTCAAGGAA 
TACCTGATTG ACCTGGCGTC CCGAGCGGGC GTGCACTGGC TGTCCACCCT CGTGCCCACC 
CACCACATCA ATGCCCTGGT CTTCTTTTTC ATCATCAGCA ACCTCACCAT CGACTTCTTC 
GCCTTCTTTG TGCCCCTGGT CGTCTTCTAC CTGTCCTTCA TCTCCATGGT CATCTGCACC 
CTCAAGGTTT TCCAGGACAG CAAGGCCTGG GAGAGCTTCC GAGCGCTCAC CAGCCTGCTC 
CTGCGCTTCG AGCCCAACCT GGACGTGGAG CAGGCCGAGG GGAACTTCGG CTGGAACCAC 
CTGGAGCCCT ACGTCCACTT CCTGCTGTCC GTCTTCTTCG TCATCATCTC CTTCCCCATC 
GCCAGCAAGG ACTGCATGCC CTGCTCGGAG CTGGCCGTCG TGTCGGTCTT CTTCACGGCC 
ACCAGCTACA TGAGCCTGAG CACCTCGGCT GAGCCCTACA CTCGGAGGGC CCTGGTGACC 
GAGGTGGCGG CCGGCCTGCT CTCCCTGCTG CCCACCATGC CCGTCAACTG GCGCTACCTG 
AAGCTCCTGG GCCAGACCTT CTTCACGGTG CCCGTGGGCC ACTTGGTCGT GCTGAACGTG 
AGCGTGCCCT GCCTGCTGTA CGTCTACCTG CTCTATCTGT TCTTCCGCAT GGCCCAGCTG 
AGGAACTTCA AAGGCACCTA CTGCTATCTG GTCCCCTACC TGGTCTGCTT CATGTGGTGC 
GAGCTGTCGG TGGTCATCCT GCTCGAGTCC ACCGGCCTGG GGCTGGTGCG GGCGTCGGTG 
GGCTACTTCC TCTTCCTCTT CGCTCTCCCC ATCCTGGTGT TCGCCCTGGC GCTGATGGGC 
GTGGTGCAGC TGGCCCGCTG GTTCGTGTCC CTGGAGCTCA CCAAGATCGC CGTCACCCTG 
GTGCTCTGCA GCGTCCCCCT GCTGGTCCGC TGGTGGACCA AGGCCAACTT CTCGGTGGTG 
GGGATGGTCA AGTCCCTGAC GCGGAGCTCC ATCGTCAAGC TCATCCTGGT GTGGCTCACG 
GCCATCGTGC TCTTCTGCTG GTTCTACCTG TACCGCTCGG AGGGCATGAA GGTCTACAAC 
TCCACACTGA CCTGGCCGCA GTACGGCTTC CTGTGCGGGC CGCGGGCCTG GAAGGAGACC 
AACATGGCCC GCACCCAGAT CCTGTGCAGC CACCTGGAGG GCCACCGGGT CACGTGGACC 
GGCCGCTTCA AGTACGTGCG CGTGACCGAG ATCGACAACA GCGCCGAGTC GGCCGTCCAC 
ATGCTGCCGC TCTTCCTGGG CGACTGGCTG CGCTGCCTCT ACGGCGAGGC CTACCCGTCC 
TGCAGCCCCG GCAACGTCTC CACGGCCGAG GAGGAGCTCT GCCGCCTCAA GCTCCTGGCC 
AAGCACCCGT GCCACATGAA GAGGTTCGAC CGCTACAAGT TCGAGATCAC AGTGGGCATG 
CCCTACAGCG GTGCCAACGG CAGCCGCGGG CCGGAGGAGG ACGACGTCAC CAAGGACATA 
GTGCTGCGGG CCAGCAGCGA GTTCAAGGAC GTGCTCCTTC ACTTGCGCCA GGGCAGCGTC 
ATCGAGTTCA GCACCGTCCT GGAGGGCCGC CTGGGCAGCA AGTGGCCCGT CTTCGAGCTC 
AAGGCCATCA GCTGCCTCAA CTGCATGGCG CACCTCGTGC CCGCCCGGCG GCACGTGAAG 
GTAGAGCGGG ACTGGCGGAG TGCCGTGCAC GGTGCCGTCA AGTTCGCCTT CGACTTCTTC 
TTCTTCCCCT TCCTGTCGGC GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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WFS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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