WFS1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	WFS1
Entrez Gene ID	422857
Full Name	Wolfram syndrome 1 (wolframin)
General protein information	Preferred Names Wolfram syndrome 1 (wolframin) Names wolframin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_420803.5	XP_420803.2	wolframin
XM_015285822.2	XP_015141308.1	wolframin
XM_015285822.1	XP_015141308.1	wolframin
XM_420803.6	XP_420803.2	wolframin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WFS1	XP_003310288.1
Danio rerio (zebrafish)	wfs1b	XP_005157280.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004409	XP_313695.5
Homo sapiens (human)	WFS1	NP_001139325.1
	WFS1	XP_001092811.1
Xenopus tropicalis (tropical clawed frog)	wfs1	NP_001106459.1
Canis lupus familiaris (dog)	WFS1	XP_539234.4
Bos taurus (cattle)	WFS1	XP_002688446.1
Gallus gallus (chicken)	WFS1	XP_420803.2
Drosophila melanogaster (fruit fly)	wfs1	NP_001189267.1
Mus musculus (house mouse)	Wfs1	NP_035846.1
Rattus norvegicus (Norway rat)	Wfs1	NP_114011.1

Gene Ontology

The following WFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa08533	XM_015285822.2 Latest version!	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OGa08533	XM_420803.6 Latest version!	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OGa08533	XM_015285822.1	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OGa08533	XM_420803.5	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WFS1 ( XM_420803.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420803.2 Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa08533
Clone ID Related Accession (Same CDS sequence)	XM_420803.5 , XM_015285822.2 , XM_015285822.1 , XM_420803.6
Accession Version	XM_420803.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455863.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420803.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420803.2
CDS	349..2973
Translation	MNSNSDPPSNPSQPQLHPGRSQLNAAAVDRSESRHKSGPSGDAATLSSSVPGYSQSREKAEKNETMKEEPEVLFEELLERAKAGEPKAQTEVGKHFLRLAEEEDEELNNCSAVDWFILAAKQGRREAVKLLRRCLADRRGITSENEQEVKKLSSETDLERAVRKAALVMYWKLNPKKKKQLAVSELLENVGQVDNEDGEKQPGPVPKSVQKQRRMLERLVSCESKKFIALDDFVEITKKYAKGIIPSNLIMQEEEDDELAGKTPEELPMRLKVIKYPLHAIMEIKEYLIDIASKAGMHWLSTIIPTHHINALIFFFIISNLTIDFFAFIIPLVIFYLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYIYFLLSVFFVIFSFPIASKDCIPCSELAVVSVFFTVTSYMSLSTCAEPYTRRALMTEIAAGCLSLLQILPGNFGFLKFLGKTYFTIPVGHLLVINVSIPCLLFLYLFYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILRESSGIGLVRASIGYFLFLFALPVLGVGIALMCLVHFIKWFVSLELVKIVVTLVLCAVPLLFRWWTKVNFSVVEMVKSLTRSSIVKLILVWITAVVLFCWFYVYRSEGMKVYNSTLTWNQYAFLCGPRSWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAINMLPLFIGDWMRCLYGETYPLCDPKNVTMEEEELCRLKYLTKHNCHMKMFDRYKFEITVGMPFSSKNGTKLVEEDDITKDIVLKASNEFKKVLLNLRQGSIIEFSTILEGRLGSKWPVFELKAITCLNCMSKLLPAGRHVKIEHDWRSTVHKAIKFVFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_420803.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420803.5

ORF Insert Sequence:

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_015285822.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141308.1 Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa08533
Clone ID Related Accession (Same CDS sequence)	XM_420803.5 , XM_015285822.2 , XM_015285822.1 , XM_420803.6
Accession Version	XM_015285822.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141308.1
CDS	98..2722
Translation	MNSNSDPPSNPSQPQLHPGRSQLNAAAVDRSESRHKSGPSGDAATLSSSVPGYSQSREKAEKNETMKEEPEVLFEELLERAKAGEPKAQTEVGKHFLRLAEEEDEELNNCSAVDWFILAAKQGRREAVKLLRRCLADRRGITSENEQEVKKLSSETDLERAVRKAALVMYWKLNPKKKKQLAVSELLENVGQVDNEDGEKQPGPVPKSVQKQRRMLERLVSCESKKFIALDDFVEITKKYAKGIIPSNLIMQEEEDDELAGKTPEELPMRLKVIKYPLHAIMEIKEYLIDIASKAGMHWLSTIIPTHHINALIFFFIISNLTIDFFAFIIPLVIFYLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYIYFLLSVFFVIFSFPIASKDCIPCSELAVVSVFFTVTSYMSLSTCAEPYTRRALMTEIAAGCLSLLQILPGNFGFLKFLGKTYFTIPVGHLLVINVSIPCLLFLYLFYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILRESSGIGLVRASIGYFLFLFALPVLGVGIALMCLVHFIKWFVSLELVKIVVTLVLCAVPLLFRWWTKVNFSVVEMVKSLTRSSIVKLILVWITAVVLFCWFYVYRSEGMKVYNSTLTWNQYAFLCGPRSWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAINMLPLFIGDWMRCLYGETYPLCDPKNVTMEEEELCRLKYLTKHNCHMKMFDRYKFEITVGMPFSSKNGTKLVEEDDITKDIVLKASNEFKKVLLNLRQGSIIEFSTILEGRLGSKWPVFELKAITCLNCMSKLLPAGRHVKIEHDWRSTVHKAIKFVFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_015285822.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285822.2

ORF Insert Sequence:

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_015285822.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141308.1 Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa08533
Clone ID Related Accession (Same CDS sequence)	XM_420803.5 , XM_015285822.2 , XM_015285822.1 , XM_420803.6
Accession Version	XM_015285822.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455863.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141308.1
CDS	93..2717
Translation	MNSNSDPPSNPSQPQLHPGRSQLNAAAVDRSESRHKSGPSGDAATLSSSVPGYSQSREKAEKNETMKEEPEVLFEELLERAKAGEPKAQTEVGKHFLRLAEEEDEELNNCSAVDWFILAAKQGRREAVKLLRRCLADRRGITSENEQEVKKLSSETDLERAVRKAALVMYWKLNPKKKKQLAVSELLENVGQVDNEDGEKQPGPVPKSVQKQRRMLERLVSCESKKFIALDDFVEITKKYAKGIIPSNLIMQEEEDDELAGKTPEELPMRLKVIKYPLHAIMEIKEYLIDIASKAGMHWLSTIIPTHHINALIFFFIISNLTIDFFAFIIPLVIFYLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYIYFLLSVFFVIFSFPIASKDCIPCSELAVVSVFFTVTSYMSLSTCAEPYTRRALMTEIAAGCLSLLQILPGNFGFLKFLGKTYFTIPVGHLLVINVSIPCLLFLYLFYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILRESSGIGLVRASIGYFLFLFALPVLGVGIALMCLVHFIKWFVSLELVKIVVTLVLCAVPLLFRWWTKVNFSVVEMVKSLTRSSIVKLILVWITAVVLFCWFYVYRSEGMKVYNSTLTWNQYAFLCGPRSWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAINMLPLFIGDWMRCLYGETYPLCDPKNVTMEEEELCRLKYLTKHNCHMKMFDRYKFEITVGMPFSSKNGTKLVEEDDITKDIVLKASNEFKKVLLNLRQGSIIEFSTILEGRLGSKWPVFELKAITCLNCMSKLLPAGRHVKIEHDWRSTVHKAIKFVFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_015285822.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285822.1

ORF Insert Sequence:

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WFS1 ( XM_420803.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420803.2 Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa08533
Clone ID Related Accession (Same CDS sequence)	XM_420803.5 , XM_015285822.2 , XM_015285822.1 , XM_420803.6
Accession Version	XM_420803.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420803.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420803.2
CDS	358..2982
Translation	MNSNSDPPSNPSQPQLHPGRSQLNAAAVDRSESRHKSGPSGDAATLSSSVPGYSQSREKAEKNETMKEEPEVLFEELLERAKAGEPKAQTEVGKHFLRLAEEEDEELNNCSAVDWFILAAKQGRREAVKLLRRCLADRRGITSENEQEVKKLSSETDLERAVRKAALVMYWKLNPKKKKQLAVSELLENVGQVDNEDGEKQPGPVPKSVQKQRRMLERLVSCESKKFIALDDFVEITKKYAKGIIPSNLIMQEEEDDELAGKTPEELPMRLKVIKYPLHAIMEIKEYLIDIASKAGMHWLSTIIPTHHINALIFFFIISNLTIDFFAFIIPLVIFYLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYIYFLLSVFFVIFSFPIASKDCIPCSELAVVSVFFTVTSYMSLSTCAEPYTRRALMTEIAAGCLSLLQILPGNFGFLKFLGKTYFTIPVGHLLVINVSIPCLLFLYLFYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILRESSGIGLVRASIGYFLFLFALPVLGVGIALMCLVHFIKWFVSLELVKIVVTLVLCAVPLLFRWWTKVNFSVVEMVKSLTRSSIVKLILVWITAVVLFCWFYVYRSEGMKVYNSTLTWNQYAFLCGPRSWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTEIDNSAESAINMLPLFIGDWMRCLYGETYPLCDPKNVTMEEEELCRLKYLTKHNCHMKMFDRYKFEITVGMPFSSKNGTKLVEEDDITKDIVLKASNEFKKVLLNLRQGSIIEFSTILEGRLGSKWPVFELKAITCLNCMSKLLPAGRHVKIEHDWRSTVHKAIKFVFDFFFFPFLSAA

Target ORF information:

RefSeq Version	XM_420803.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Wolfram syndrome 1 (wolframin) (WFS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420803.6

ORF Insert Sequence:

ATGAACTCAA ATTCTGATCC TCCATCAAAT CCCTCTCAAC CGCAACTGCA TCCTGGAAGG 
TCCCAGCTGA ACGCTGCTGC TGTGGATCGT AGTGAGAGCC GTCATAAATC TGGACCCTCA 
GGAGATGCTG CAACTCTTTC TTCTTCAGTT CCAGGCTACT CCCAAAGCAG GGAGAAAGCT 
GAAAAAAACG AGACCATGAA GGAGGAACCT GAAGTGCTCT TTGAGGAACT GCTGGAGAGG 
GCCAAAGCTG GAGAACCAAA AGCACAAACA GAGGTGGGGA AACACTTCTT GAGATTAGCA 
GAAGAGGAGG ATGAAGAACT TAACAACTGT AGTGCGGTTG ATTGGTTCAT CCTTGCTGCT 
AAGCAAGGTC GAAGAGAAGC TGTTAAACTG TTGCGTAGAT GCCTAGCAGA CAGAAGAGGC 
ATCACTTCTG AGAATGAGCA AGAAGTTAAA AAACTATCAT CTGAGACTGA CTTGGAGAGA 
GCTGTGAGAA AAGCTGCCTT AGTCATGTAT TGGAAATTAA ACCCAAAAAA GAAGAAGCAA 
TTAGCAGTTT CTGAACTACT GGAAAATGTT GGGCAAGTTG ATAATGAAGA TGGTGAGAAG 
CAACCTGGTC CAGTCCCAAA GTCAGTGCAG AAGCAGAGGA GAATGCTGGA GCGCTTAGTG 
AGCTGTGAAT CTAAAAAATT TATTGCTTTG GATGACTTCG TGGAGATTAC CAAGAAGTAT 
GCAAAGGGAA TCATCCCATC TAACCTGATT ATGCAGGAAG AGGAAGATGA TGAATTGGCA 
GGGAAGACTC CTGAAGAGTT GCCCATGAGA CTGAAGGTTA TAAAATATCC ACTTCATGCC 
ATAATGGAAA TTAAAGAATA TCTTATAGAT ATTGCATCAA AGGCAGGAAT GCATTGGCTG 
TCTACCATTA TTCCAACACA TCACATCAAT GCTCTCATCT TCTTCTTCAT CATCAGTAAT 
CTGACAATTG ATTTTTTTGC CTTCATTATT CCGTTAGTTA TATTCTATTT GTCCTTCATT 
TCTATGGTGA TTTGTACACT GAAAGTTTTT CAGGACAGTA AGGCCTGGGA AAACTTCCGC 
ACTTTGACTG ACTTACTCCT TCGTTTTGAA CCAAACCTAG ATGTTGAGCA AGCTGAGGTG 
AACTTTGGGT GGAATCACTT AGAGCCATAC ATTTATTTTT TACTCTCAGT GTTCTTTGTA 
ATTTTTTCCT TCCCTATAGC AAGCAAGGAC TGTATACCAT GCTCAGAGTT AGCTGTTGTC 
TCAGTTTTCT TCACAGTGAC AAGCTACATG AGTTTAAGCA CATGTGCAGA ACCGTACACG 
CGAAGAGCAT TAATGACTGA GATAGCAGCA GGTTGCTTAT CCCTGTTGCA GATATTACCT 
GGGAATTTTG GCTTCTTGAA ATTCTTAGGT AAAACCTACT TTACTATTCC TGTAGGCCAT 
CTTTTGGTGA TCAATGTAAG CATCCCGTGC CTTTTGTTTT TGTATTTGTT CTATCTTTTC 
TTCAGAATGG CACAACTGCG AAATTTTAAA GGCACCTACT GTTACCTGGT CCCATATCTG 
GTCTGCTTTA TGTGGTGTGA ACTCTCTGTG GTCATTCTGC GGGAGTCCTC TGGCATTGGG 
CTCGTCCGTG CATCAATTGG TTACTTTCTC TTTCTCTTTG CACTCCCAGT GCTGGGTGTA 
GGCATTGCAC TGATGTGTCT GGTCCATTTC ATTAAGTGGT TCGTGTCTCT GGAGCTCGTG 
AAAATTGTAG TGACTCTAGT TTTGTGTGCT GTTCCTTTGC TCTTCCGATG GTGGACAAAA 
GTCAACTTCT CTGTAGTAGA AATGGTTAAA TCCCTCACTC GAAGCTCAAT TGTGAAACTC 
ATTTTGGTGT GGATTACAGC TGTAGTGTTG TTCTGTTGGT TCTATGTGTA TCGATCAGAG 
GGAATGAAAG TTTACAACTC CACCTTGACA TGGAATCAGT ATGCCTTCCT CTGTGGACCC 
CGATCGTGGA AGGAGACCAA TATGGCACGT ACTCAGATTT TATGTAGTCA CCTGGAAGGA 
CACAGAGTGA CGTGGACTGG GCGGTTCAAA TACGTGCGTG TTACAGAAAT TGACAATAGT 
GCAGAATCTG CAATAAATAT GCTTCCACTT TTTATTGGTG ATTGGATGAG GTGCTTGTAT 
GGTGAAACCT ACCCTCTTTG TGACCCCAAA AATGTCACTA TGGAGGAGGA AGAATTGTGT 
CGTCTCAAGT ATCTGACAAA GCATAACTGC CACATGAAAA TGTTCGATCG GTACAAATTT 
GAAATAACTG TGGGTATGCC TTTCAGTAGC AAAAATGGAA CCAAGTTGGT GGAGGAAGAT 
GACATAACCA AAGATATTGT GCTGAAGGCA AGCAATGAGT TTAAGAAAGT GTTGTTGAAC 
TTGAGGCAAG GAAGTATAAT TGAATTCAGC ACAATTCTGG AAGGTCGTCT TGGCAGTAAA 
TGGCCTGTCT TTGAACTGAA AGCAATCACT TGCTTGAATT GCATGTCTAA ACTCCTACCT 
GCAGGGAGGC ATGTGAAAAT AGAGCATGAC TGGAGGAGCA CAGTGCATAA AGCCATTAAA 
TTTGTTTTTG ATTTTTTCTT CTTCCCATTC CTGTCAGCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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WFS1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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