st8sia1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	st8sia1
Entrez Gene ID	553210
Full Name	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Synonyms	siat 8A,zgc:158304
General protein information	Preferred Names ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 Names alpha-N-acetylneuraminide alpha-2,8-sialyltransferase alpha-2,8-sialyltransferase ST8Sia I
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001327841.1	NP_001314770.1	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 1
NM_001081632.1	NP_001075101.1	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Mapping the expressed glycome and glycosyltransferases of zebrafish liver cells as a relevant model system for glycosylation studies.
Vanbeselaere J, Chang LY, Harduin-Lepers A, Fabre E, Yamakawa N, Slomianny C, Biot C, Khoo KH, Guerardel Y
Journal of proteome research11(4)2164-77(2012 Apr)

Evolutionary history of the alpha2,8-sialyltransferase (ST8Sia) gene family: tandem duplications in early deuterostomes explain most of the diversity found in the vertebrate ST8Sia genes.
Harduin-Lepers A, Petit D, Mollicone R, Delannoy P, Petit JM, Oriol R
BMC evolutionary biology8258(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following st8sia1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the st8sia1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa29837	NM_001327841.1 Latest version!	Danio rerio ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (st8sia1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa31244	NM_001081632.1 Latest version!	Danio rerio ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (st8sia1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

st8sia1 ( NM_001327841.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001314770.1 Danio rerio ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (st8sia1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa29837
Clone ID Related Accession (Same CDS sequence)	NM_001327841.1
Accession Version	NM_001327841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-27
Organism	Danio rerio(zebrafish)
Product	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU137645.14 and CT573128.9. On May 26, 2016 this sequence version replaced XM_005170806.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01017658.1, AJ715535.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505372, SAMEA3505387 [ECO:0000348] ##Evidence-Data-END##

ATGGTGTCTT TGAGATGCCA CAGGAGCAAG TATATTTGGG CCACTCTGGG GGTGCTGGCG 
TTGTTGTGGC TGTACATCTT CCCCGTATAC AGGATACCCA GCGACAAGGA AATGGTGGAG 
GAGGTTCTGC GGCAAGGACA GACATGGAGC AGGAACCAGA CCGCGGTGGA ACTGTACAGG 
AAGCTGCTGA CAGATTGCTG TAACCCAAAG AGGATGTTTG CGGTTACTAA GGAGAATTCG 
CCATTAGGCA AAGTCTTGTG GTATGATGGT GAATTCTACC ACTATCACAC AGTCACCAAT 
GAGACCTACC CCATCTTTGT ACAGGATACA CCATTGCAGC TTCCTCTAAA GAGGTGTTCT 
GTTGTGGGTA ATGGAGGGGT GCTGAAACAT AGTGGCTGTG GAAATGAAAT TGACCGAGCT 
GACTTTATCA TGAGATGTAA CCTTCCTCCT CTGTCTAAAG ATTATACTGA TGATGTTGGT 
ACCAAAACAC ACCTGGTGAG CGCGAATCCA AGTATTATAG AAAAGAGCTT TCAGAACCTA 
CTTTGGTCCC GCAAATCTTT CGTAGAGAGC ATGAAGGCCT ATGGTTCCAG CTACATCTAC 
ATACCAGCGT TCTCTATGAA GCCTGGCACT GACCCGTCAT TACGAGCATA CCACGCACTC 
GCAGACTCCT CCTCCAATCA GACTGTCCTC TTTGCTAACC CAGATTTCCT TAAAAATGTT 
GGCATATTCT GGAAAAACCA TGGCGTTCAT GGCAAGCGAT TATCCACAGG GTTATTCTTA 
GTGAGCCTAG CCCTTGGCTT ATGTGAAGAG GTGACAGCTT ATGGTTTTTG GCCATTTTCA 
GTGGGCCTAG ATGAGCGACC TGTCAGCCAC CACTACTATG ACAACATTCT ACCATCCTCA 
AGATTTCATG CCATGCCTGA GGAGTTTCTT CAACTCTGGC ATTTGCATAA AAGCGGTACA 
TTGCGGATGC GGGTTGGCAG TTGTGCAAAA GAGCGGATGG AGTTAAAGAG GGAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314770.1
CDS	376..1395
Translation	MVSLRCHRSKYIWATLGVLALLWLYIFPVYRIPSDKEMVEEVLRQGQTWSRNQTAVELYRKLLTDCCNPKRMFAVTKENSPLGKVLWYDGEFYHYHTVTNETYPIFVQDTPLQLPLKRCSVVGNGGVLKHSGCGNEIDRADFIMRCNLPPLSKDYTDDVGTKTHLVSANPSIIEKSFQNLLWSRKSFVESMKAYGSSYIYIPAFSMKPGTDPSLRAYHALADSSSNQTVLFANPDFLKNVGIFWKNHGVHGKRLSTGLFLVSLALGLCEEVTAYGFWPFSVGLDERPVSHHYYDNILPSSRFHAMPEEFLQLWHLHKSGTLRMRVGSCAKERMELKREK

Target ORF information:

RefSeq Version	NM_001327841.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (st8sia1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327841.1

ORF Insert Sequence:

ATGGTGTCTT TGAGATGCCA CAGGAGCAAG TATATTTGGG CCACTCTGGG GGTGCTGGCG 
TTGTTGTGGC TGTACATCTT CCCCGTATAC AGGATACCCA GCGACAAGGA AATGGTGGAG 
GAGGTTCTGC GGCAAGGACA GACATGGAGC AGGAACCAGA CCGCGGTGGA ACTGTACAGG 
AAGCTGCTGA CAGATTGCTG TAACCCAAAG AGGATGTTTG CGGTTACTAA GGAGAATTCG 
CCATTAGGCA AAGTCTTGTG GTATGATGGT GAATTCTACC ACTATCACAC AGTCACCAAT 
GAGACCTACC CCATCTTTGT ACAGGATACA CCATTGCAGC TTCCTCTAAA GAGGTGTTCT 
GTTGTGGGTA ATGGAGGGGT GCTGAAACAT AGTGGCTGTG GAAATGAAAT TGACCGAGCT 
GACTTTATCA TGAGATGTAA CCTTCCTCCT CTGTCTAAAG ATTATACTGA TGATGTTGGT 
ACCAAAACAC ACCTGGTGAG CGCGAATCCA AGTATTATAG AAAAGAGCTT TCAGAACCTA 
CTTTGGTCCC GCAAATCTTT CGTAGAGAGC ATGAAGGCCT ATGGTTCCAG CTACATCTAC 
ATACCAGCGT TCTCTATGAA GCCTGGCACT GACCCGTCAT TACGAGCATA CCACGCACTC 
GCAGACTCCT CCTCCAATCA GACTGTCCTC TTTGCTAACC CAGATTTCCT TAAAAATGTT 
GGCATATTCT GGAAAAACCA TGGCGTTCAT GGCAAGCGAT TATCCACAGG GTTATTCTTA 
GTGAGCCTAG CCCTTGGCTT ATGTGAAGAG GTGACAGCTT ATGGTTTTTG GCCATTTTCA 
GTGGGCCTAG ATGAGCGACC TGTCAGCCAC CACTACTATG ACAACATTCT ACCATCCTCA 
AGATTTCATG CCATGCCTGA GGAGTTTCTT CAACTCTGGC ATTTGCATAA AAGCGGTACA 
TTGCGGATGC GGGTTGGCAG TTGTGCAAAA GAGCGGATGG AGTTAAAGAG GGAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

st8sia1 ( NM_001081632.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001075101.1 Danio rerio ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (st8sia1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa31244
Clone ID Related Accession (Same CDS sequence)	NM_001081632.1
Accession Version	NM_001081632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Danio rerio(zebrafish)
Product	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128837.1. On Mar 11, 2007 this sequence version replaced XM_684714.2. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC128837.1, CD755089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGGTGTCTT TGAGATGCCA CAGGAGCAAG TATATTTGGG CCACTCTGGG GGTGCTGGCG 
TTGTTGTGGC TGTACATCTT CCCCGTATAC AGGATACCCA GCGACAAGGA AATGGTGGAG 
GAGGTTCTGC GGCAAGGACA GACATGGAGC AGGAACCAGA CCGCGGTGGA ACTGTACAGG 
AAGCTGCTGA CAGATTGCTG TAACCCAAAG AGGATGTTTG CGGTTACTAA GGAGAATTCG 
CCATTAGGCA AAGTCTTGTG GTATGATGGT GAATTCTACC ACTATCACAC AGTCACCAAT 
GAGACCTACC CCATCTTTGT ACAGGATACA CCATTGCAGC TTCCTCTAAA GAGGTGTTCT 
GTTGTGGGTA ATGGAGGGGT GCTGAAACAT AGTGGCTGTG GAAATGAAAT TGACCGAGCT 
GACTTTATCA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075101.1
CDS	379..816
Translation	MVSLRCHRSKYIWATLGVLALLWLYIFPVYRIPSDKEMVEEVLRQGQTWSRNQTAVELYRKLLTDCCNPKRMFAVTKENSPLGKVLWYDGEFYHYHTVTNETYPIFVQDTPLQLPLKRCSVVGNGGVLKHSGCGNEIDRADFIMR

Target ORF information:

RefSeq Version	NM_001081632.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (st8sia1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081632.1

ORF Insert Sequence:

ATGGTGTCTT TGAGATGCCA CAGGAGCAAG TATATTTGGG CCACTCTGGG GGTGCTGGCG 
TTGTTGTGGC TGTACATCTT CCCCGTATAC AGGATACCCA GCGACAAGGA AATGGTGGAG 
GAGGTTCTGC GGCAAGGACA GACATGGAGC AGGAACCAGA CCGCGGTGGA ACTGTACAGG 
AAGCTGCTGA CAGATTGCTG TAACCCAAAG AGGATGTTTG CGGTTACTAA GGAGAATTCG 
CCATTAGGCA AAGTCTTGTG GTATGATGGT GAATTCTACC ACTATCACAC AGTCACCAAT 
GAGACCTACC CCATCTTTGT ACAGGATACA CCATTGCAGC TTCCTCTAAA GAGGTGTTCT 
GTTGTGGGTA ATGGAGGGGT GCTGAAACAT AGTGGCTGTG GAAATGAAAT TGACCGAGCT 
GACTTTATCA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Mapping the expressed glycome and glycosyltransferases of zebrafish liver cells as a relevant model system for glycosylation studies.
Journal of proteome research11(4)2164-77(2012 Apr)
Vanbeselaere J,Chang LY,Harduin-Lepers A,Fabre E,Yamakawa N,Slomianny C,Biot C,Khoo KH,Guerardel Y

Evolutionary history of the alpha2,8-sialyltransferase (ST8Sia) gene family: tandem duplications in early deuterostomes explain most of the diversity found in the vertebrate ST8Sia genes.
BMC evolutionary biology8258(2008 Sep)
Harduin-Lepers A,Petit D,Mollicone R,Delannoy P,Petit JM,Oriol R