selenof cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenof
Entrez Gene ID	352923
Full Name	selenoprotein F
Synonyms	cb29,sep15,zgc:86882
General protein information	Preferred Names selenoprotein F Names selenoprotein F 15 kDa selenoprotein selenoprotein 15
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178294.5	NP_840079.1	selenoprotein F precursor

Pathways from BioSystems

Homologies

	SEP15	NP_001152888.1
Bos taurus (cattle)	SEP15	NP_001029931.2
Gallus gallus (chicken)	SEP15	NP_001012944.2
Arabidopsis thaliana (thale cress)	AT1G05720	NP_563747.1
Xenopus tropicalis (tropical clawed frog)	sep15	NP_001165182.1
Homo sapiens (human)	SEP15	NP_004252.2
Pan troglodytes (chimpanzee)	SEP15	NP_001139315.1
Rattus norvegicus (Norway rat)	Sep15	NP_579831.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004986	XP_315090.2
Oryza sativa (rice)	Os01g0894500	NP_001045069.1
Canis lupus familiaris (dog)	SEP15	NP_001108232.1
Mus musculus (house mouse)	Sep15	NP_444332.1
Danio rerio (zebrafish)	sep15	NP_840079.1
Drosophila melanogaster (fruit fly)	CG7484	NP_649000.1
Caenorhabditis elegans (roundworm)	Y76B12C.3	NP_500152.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress.
Betancor MB, Almaida-Pag?n PF, Sprague M, Hern?ndez A, Tocher DR
Fish physiology and biochemistry41(3)705-20(2015 Jun)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38997	NM_178294.5 Latest version!	Danio rerio selenoprotein F (selenof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenof ( NM_178294.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_840079.1 Danio rerio selenoprotein F (selenof), mRNA.

Price & Availability↑

CloneID	ODa38997
Clone ID Related Accession (Same CDS sequence)	NM_178294.5
Accession Version	NM_178294.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Danio rerio(zebrafish)
Product	selenoprotein F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH553008.1 and EH535677.1. On Nov 16, 2016 this sequence version replaced NM_178294.4. ##Evidence-Data-START## Transcript exon combination :: GDQH01003959.1, BC071511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG AGGTGTATCT GCTTTGGTTG CTGCCGTTAT TACAGGGGTT GGCGTCATAT 
GGAGCAGAGC TCTCATCTGA GGCTTGCAGG GAGCTGGGGT TCTCCAGCAA TCTCCTGTGT 
AGCTCCTGTG AGCTTCTGGG TCAGTTCAGT CTGAACCAGC TAGACCTTCC CTGCAGGCAG 
TGCTGCCAAG AGGAGGCTCA GCTGGAGAAC AGGAAGCTCT ACCCAGGAGC CATCCTTGAG 
GTCTGTGGAT GAAAACTGGG GAGGTTCCCT CAAGTCCAAG CTTTTGTCAG GAGTGACAAG 
CCAAAGTTGT TCAGAGGGCT TCAAATCAAG TATGTGAGAG GCTCGGACCC TGTACTAAAG 
CTGTTGGACG ATAACGGGAA CATCGCTGAA GAGCTCAGCA TCCTCAAGTG GAACACAGAC 
AGCGTTGAAG AATTTCTTAG TGAGAAGTTA GAGCGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840079.1
CDS	55..516
Translation	MAGEVYLLWLLPLLQGLASYGAELSSEACRELGFSSNLLCSSCELLGQFSLNQLDLPCRQCCQEEAQLENRKLYPGAILEVCG*KLGRFPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI

Target ORF information:

RefSeq Version	NM_178294.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein F (selenof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178294.5

ORF Insert Sequence:

ATGGCGGGGG AGGTGTATCT GCTTTGGTTG CTGCCGTTAT TACAGGGGTT GGCGTCATAT 
GGAGCAGAGC TCTCATCTGA GGCTTGCAGG GAGCTGGGGT TCTCCAGCAA TCTCCTGTGT 
AGCTCCTGTG AGCTTCTGGG TCAGTTCAGT CTGAACCAGC TAGACCTTCC CTGCAGGCAG 
TGCTGCCAAG AGGAGGCTCA GCTGGAGAAC AGGAAGCTCT ACCCAGGAGC CATCCTTGAG 
GTCTGTGGAT GAAAACTGGG GAGGTTCCCT CAAGTCCAAG CTTTTGTCAG GAGTGACAAG 
CCAAAGTTGT TCAGAGGGCT TCAAATCAAG TATGTGAGAG GCTCGGACCC TGTACTAAAG 
CTGTTGGACG ATAACGGGAA CATCGCTGAA GAGCTCAGCA TCCTCAAGTG GAACACAGAC 
AGCGTTGAAG AATTTCTTAG TGAGAAGTTA GAGCGAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress.
Fish physiology and biochemistry41(3)705-20(2015 Jun)
Betancor MB,Almaida-Pag?n PF,Sprague M,Hern?ndez A,Tocher DR

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

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selenof cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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