usp53b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp53b
Entrez Gene ID	100537347
Full Name	ubiquitin specific peptidase 53b
Synonyms	si:ch73-140j6.1
General protein information	Preferred Names ubiquitin specific peptidase 53b Names inactive ubiquitin carboxyl-terminal hydrolase 53 uncharacterized protein usp53b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021471203.1	XP_021326878.1	uncharacterized protein usp53b isoform X4
XM_009291397.2	XP_009289672.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
XM_009291397.3	XP_009289672.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
XM_021476531.1	XP_021332206.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
XM_005170957.3	XP_005171014.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_005170957.4	XP_005171014.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1

Pathways from BioSystems
Gene Ontology

The following usp53b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp53b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa50503	XM_009291397.3 Latest version!	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa144257	XM_021471203.1 Latest version!	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
ODa147405	XM_021476531.1 Latest version!	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa05734	XM_005170957.4 Latest version!	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa50503	XM_009291397.2	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa05734	XM_005170957.3	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp53b ( XM_009291397.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289672.1 Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X2, mRNA.

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CloneID	ODa50503
Clone ID Related Accession (Same CDS sequence)	XM_009291397.2 , XM_009291397.3
Accession Version	XM_009291397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC TCCCCCGGCT GTCATTCTCA CAGAGTCCTA TTGGAATGGG AAAGCATTCT 
TATTTTAATA TGAATATCTA CACTCAGCTG TCAGGCTGTA AGAAACTGGA CAGTGTGAGA 
GATGCGAGTG CTGGTTCCCA ACGAACAAAT CAGGGATCTC ACAAAACACC AATGCAGTCT 
TCAACCAGCC GGCTTAAAGT GCTTCCAGAA AGTCCAAGCA GATTCCGAGA GTCTTCCAGA 
GATTTGTCTC ACAGAGGAGG AGGAGGACAG AGGAGAGAGA GTGATAGAAG CTACCGAAGA 
TCAGACTCTT TCCAACACAA AGATATGACA TATCCACGCT TATCCTCTCC ACCTGAGAAT 
GGCCCAAGGT TTCATTGTGA GCAGAGGCCG CGAACTTTGC CCTCACGGGT CTCTCGAACT 
GAGCACTCTT CTGTTCATCC TCGTGTATCA CCCCAGGAGT CTCGCTCAAA CCAGGGGATG 
GCATCTGGAG TCAGTCGTCA CGCTGATTCA TCATCGCTTT CCCATCGTGG CTCCACAAAT 
CCCACTGAGC GTGGCAAGTC CAAGCCCTCA TGGAGACCAG TACGGGAGGT CCTCAATGTA 
GACAGTGTGC TGAGCGAACT AGAGCGACGA CAGCAGCAGC AACAGCAGCA ACAGCGTGGA 
AGCCCTCGAT GTGTGCGTCG ATTCGCCCAT GAGAGGGGGC TGATGAAGGA CAAGCAGAAG 
GGTCTGATGA CTATTTATGA AGATGACTCA CGGCTGGAGA CTGAAAGTCA AAGCTCCCAG 
GAGTCTCAAC GACAGATGAT GGTGACCAGG TCTAAAGGTG GAGCAAATGT AGCACAGCGA 
AGTGATAACT GGGTAATACA GAGGACAGAG TCAGGATATG AGAGCAGTGA TAGGCTGAGC 
AGCGGCTCTA CCAATCCTGA CTCACCTGGA GTTGAGAACT TTACCGGCAA AGAGCTGAGA 
CTCAGTCAAG ATGCACAGCA CCTCAGGGAT CAAGGCCATA ATAATCAAGA TGACTCAAAA 
ATGGAGGCGG CGCGTTCTCC ACTGTACCAC GGTAAACACT TGGCTAAAAT TCAGGGAAAG 
AACTCAGAAC ATCTCATGAA AGGCAGTCCA AGTTCAAGAA GGAAGCTTCG ATATTCATCC 
TGTGGTACCA GGAAAGCAGG CAACACAGAG AAAGACCCGT CAGGTGTGGA TCTTCCACCA 
GAGCAGCAGG AACTTTCCAT GCAATGGCAC CACAGCCCAG AAAGCCCATT ACTGCGCCGC 
CTGGGCAAGA TCAGCAGTTC AGAGTGTAAC AGTTCTGATG AACTCACTAA CCCTCTGTCA 
GAGCAGGAGG ACAGTGCTTA TCGTTCCAGC ACCAGTGAAG TCGCTCCACA TGAGTCACCG 
GGTCCCAATC GCTCTAGTTA CCCATGGGAA GAATCAGCCA TCCAATCAGG ACCCTCGATT 
GCAAGCCACT CCAAACACGC TCAGCGACCG ATCGAGATAA AGACTCCACC TTTCCGCCCC 
AGAATCTTAC AGGAACCATC TCAAACAGCT TCAAGAGCAT CTGGCCGTGG TGCTCCATCA 
TCTTCACACA GTGGCGCAGT ACACAGGCGA ATGGAGATCA ATGGCACGTG CCCCAGCTCG 
TCAGACGTGA GCTCAAAATC GGGATCAGAC CAGGAAAGAG GGGAGCAGGT GTCCAGCGAG 
CCCGAGGGTT GGGATAAGCC ATCTTCTTCT GTGTCTCCAG GATCTGGAGT GGCGCTGACC 
ACTTATTTCT CTGTGGATAA TTGCATGACA GACACCTACA GACTGAAGTA TCACCACCAG 
AGGCCATTGG TCCTCTCCAT GCCTGAGCCT AGAGGAGCTG GTAAGGACGG GAGGGATGCG 
GGACACTCCA GCCGCATGGA GAACCAGTCT GAGCGTGCCA AAAATAAGCC TGAGTCCGGC 
AATAGCAACA ATAAGCCCAC TGCTAAATGG CACCCTGTCA CTTCAAAAGG GCTAGATGAA 
CATGGATATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289672.1
CDS	120..2114
Translation	MDILPRLSFSQSPIGMGKHSYFNMNIYTQLSGCKKLDSVRDASAGSQRTNQGSHKTPMQSSTSRLKVLPESPSRFRESSRDLSHRGGGGQRRESDRSYRRSDSFQHKDMTYPRLSSPPENGPRFHCEQRPRTLPSRVSRTEHSSVHPRVSPQESRSNQGMASGVSRHADSSSLSHRGSTNPTERGKSKPSWRPVREVLNVDSVLSELERRQQQQQQQQRGSPRCVRRFAHERGLMKDKQKGLMTIYEDDSRLETESQSSQESQRQMMVTRSKGGANVAQRSDNWVIQRTESGYESSDRLSSGSTNPDSPGVENFTGKELRLSQDAQHLRDQGHNNQDDSKMEAARSPLYHGKHLAKIQGKNSEHLMKGSPSSRRKLRYSSCGTRKAGNTEKDPSGVDLPPEQQELSMQWHHSPESPLLRRLGKISSSECNSSDELTNPLSEQEDSAYRSSTSEVAPHESPGPNRSSYPWEESAIQSGPSIASHSKHAQRPIEIKTPPFRPRILQEPSQTASRASGRGAPSSSHSGAVHRRMEINGTCPSSSDVSSKSGSDQERGEQVSSEPEGWDKPSSSVSPGSGVALTTYFSVDNCMTDTYRLKYHHQRPLVLSMPEPRGAGKDGRDAGHSSRMENQSERAKNKPESGNSNNKPTAKWHPVTSKGLDEHGYL

Target ORF information:

RefSeq Version	XM_009291397.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291397.2

ORF Insert Sequence:

ATGGATATTC TCCCCCGGCT GTCATTCTCA CAGAGTCCTA TTGGAATGGG AAAGCATTCT 
TATTTTAATA TGAATATCTA CACTCAGCTG TCAGGCTGTA AGAAACTGGA CAGTGTGAGA 
GATGCGAGTG CTGGTTCCCA ACGAACAAAT CAGGGATCTC ACAAAACACC AATGCAGTCT 
TCAACCAGCC GGCTTAAAGT GCTTCCAGAA AGTCCAAGCA GATTCCGAGA GTCTTCCAGA 
GATTTGTCTC ACAGAGGAGG AGGAGGACAG AGGAGAGAGA GTGATAGAAG CTACCGAAGA 
TCAGACTCTT TCCAACACAA AGATATGACA TATCCACGCT TATCCTCTCC ACCTGAGAAT 
GGCCCAAGGT TTCATTGTGA GCAGAGGCCG CGAACTTTGC CCTCACGGGT CTCTCGAACT 
GAGCACTCTT CTGTTCATCC TCGTGTATCA CCCCAGGAGT CTCGCTCAAA CCAGGGGATG 
GCATCTGGAG TCAGTCGTCA CGCTGATTCA TCATCGCTTT CCCATCGTGG CTCCACAAAT 
CCCACTGAGC GTGGCAAGTC CAAGCCCTCA TGGAGACCAG TACGGGAGGT CCTCAATGTA 
GACAGTGTGC TGAGCGAACT AGAGCGACGA CAGCAGCAGC AACAGCAGCA ACAGCGTGGA 
AGCCCTCGAT GTGTGCGTCG ATTCGCCCAT GAGAGGGGGC TGATGAAGGA CAAGCAGAAG 
GGTCTGATGA CTATTTATGA AGATGACTCA CGGCTGGAGA CTGAAAGTCA AAGCTCCCAG 
GAGTCTCAAC GACAGATGAT GGTGACCAGG TCTAAAGGTG GAGCAAATGT AGCACAGCGA 
AGTGATAACT GGGTAATACA GAGGACAGAG TCAGGATATG AGAGCAGTGA TAGGCTGAGC 
AGCGGCTCTA CCAATCCTGA CTCACCTGGA GTTGAGAACT TTACCGGCAA AGAGCTGAGA 
CTCAGTCAAG ATGCACAGCA CCTCAGGGAT CAAGGCCATA ATAATCAAGA TGACTCAAAA 
ATGGAGGCGG CGCGTTCTCC ACTGTACCAC GGTAAACACT TGGCTAAAAT TCAGGGAAAG 
AACTCAGAAC ATCTCATGAA AGGCAGTCCA AGTTCAAGAA GGAAGCTTCG ATATTCATCC 
TGTGGTACCA GGAAAGCAGG CAACACAGAG AAAGACCCGT CAGGTGTGGA TCTTCCACCA 
GAGCAGCAGG AACTTTCCAT GCAATGGCAC CACAGCCCAG AAAGCCCATT ACTGCGCCGC 
CTGGGCAAGA TCAGCAGTTC AGAGTGTAAC AGTTCTGATG AACTCACTAA CCCTCTGTCA 
GAGCAGGAGG ACAGTGCTTA TCGTTCCAGC ACCAGTGAAG TCGCTCCACA TGAGTCACCG 
GGTCCCAATC GCTCTAGTTA CCCATGGGAA GAATCAGCCA TCCAATCAGG ACCCTCGATT 
GCAAGCCACT CCAAACACGC TCAGCGACCG ATCGAGATAA AGACTCCACC TTTCCGCCCC 
AGAATCTTAC AGGAACCATC TCAAACAGCT TCAAGAGCAT CTGGCCGTGG TGCTCCATCA 
TCTTCACACA GTGGCGCAGT ACACAGGCGA ATGGAGATCA ATGGCACGTG CCCCAGCTCG 
TCAGACGTGA GCTCAAAATC GGGATCAGAC CAGGAAAGAG GGGAGCAGGT GTCCAGCGAG 
CCCGAGGGTT GGGATAAGCC ATCTTCTTCT GTGTCTCCAG GATCTGGAGT GGCGCTGACC 
ACTTATTTCT CTGTGGATAA TTGCATGACA GACACCTACA GACTGAAGTA TCACCACCAG 
AGGCCATTGG TCCTCTCCAT GCCTGAGCCT AGAGGAGCTG GTAAGGACGG GAGGGATGCG 
GGACACTCCA GCCGCATGGA GAACCAGTCT GAGCGTGCCA AAAATAAGCC TGAGTCCGGC 
AATAGCAACA ATAAGCCCAC TGCTAAATGG CACCCTGTCA CTTCAAAAGG GCTAGATGAA 
CATGGATATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp53b ( XM_009291397.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289672.1 Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa50503
Clone ID Related Accession (Same CDS sequence)	XM_009291397.2 , XM_009291397.3
Accession Version	XM_009291397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTC TCCCCCGGCT GTCATTCTCA CAGAGTCCTA TTGGAATGGG AAAGCATTCT 
TATTTTAATA TGAATATCTA CACTCAGCTG TCAGGCTGTA AGAAACTGGA CAGTGTGAGA 
GATGCGAGTG CTGGTTCCCA ACGAACAAAT CAGGGATCTC ACAAAACACC AATGCAGTCT 
TCAACCAGCC GGCTTAAAGT GCTTCCAGAA AGTCCAAGCA GATTCCGAGA GTCTTCCAGA 
GATTTGTCTC ACAGAGGAGG AGGAGGACAG AGGAGAGAGA GTGATAGAAG CTACCGAAGA 
TCAGACTCTT TCCAACACAA AGATATGACA TATCCACGCT TATCCTCTCC ACCTGAGAAT 
GGCCCAAGGT TTCATTGTGA GCAGAGGCCG CGAACTTTGC CCTCACGGGT CTCTCGAACT 
GAGCACTCTT CTGTTCATCC TCGTGTATCA CCCCAGGAGT CTCGCTCAAA CCAGGGGATG 
GCATCTGGAG TCAGTCGTCA CGCTGATTCA TCATCGCTTT CCCATCGTGG CTCCACAAAT 
CCCACTGAGC GTGGCAAGTC CAAGCCCTCA TGGAGACCAG TACGGGAGGT CCTCAATGTA 
GACAGTGTGC TGAGCGAACT AGAGCGACGA CAGCAGCAGC AACAGCAGCA ACAGCGTGGA 
AGCCCTCGAT GTGTGCGTCG ATTCGCCCAT GAGAGGGGGC TGATGAAGGA CAAGCAGAAG 
GGTCTGATGA CTATTTATGA AGATGACTCA CGGCTGGAGA CTGAAAGTCA AAGCTCCCAG 
GAGTCTCAAC GACAGATGAT GGTGACCAGG TCTAAAGGTG GAGCAAATGT AGCACAGCGA 
AGTGATAACT GGGTAATACA GAGGACAGAG TCAGGATATG AGAGCAGTGA TAGGCTGAGC 
AGCGGCTCTA CCAATCCTGA CTCACCTGGA GTTGAGAACT TTACCGGCAA AGAGCTGAGA 
CTCAGTCAAG ATGCACAGCA CCTCAGGGAT CAAGGCCATA ATAATCAAGA TGACTCAAAA 
ATGGAGGCGG CGCGTTCTCC ACTGTACCAC GGTAAACACT TGGCTAAAAT TCAGGGAAAG 
AACTCAGAAC ATCTCATGAA AGGCAGTCCA AGTTCAAGAA GGAAGCTTCG ATATTCATCC 
TGTGGTACCA GGAAAGCAGG CAACACAGAG AAAGACCCGT CAGGTGTGGA TCTTCCACCA 
GAGCAGCAGG AACTTTCCAT GCAATGGCAC CACAGCCCAG AAAGCCCATT ACTGCGCCGC 
CTGGGCAAGA TCAGCAGTTC AGAGTGTAAC AGTTCTGATG AACTCACTAA CCCTCTGTCA 
GAGCAGGAGG ACAGTGCTTA TCGTTCCAGC ACCAGTGAAG TCGCTCCACA TGAGTCACCG 
GGTCCCAATC GCTCTAGTTA CCCATGGGAA GAATCAGCCA TCCAATCAGG ACCCTCGATT 
GCAAGCCACT CCAAACACGC TCAGCGACCG ATCGAGATAA AGACTCCACC TTTCCGCCCC 
AGAATCTTAC AGGAACCATC TCAAACAGCT TCAAGAGCAT CTGGCCGTGG TGCTCCATCA 
TCTTCACACA GTGGCGCAGT ACACAGGCGA ATGGAGATCA ATGGCACGTG CCCCAGCTCG 
TCAGACGTGA GCTCAAAATC GGGATCAGAC CAGGAAAGAG GGGAGCAGGT GTCCAGCGAG 
CCCGAGGGTT GGGATAAGCC ATCTTCTTCT GTGTCTCCAG GATCTGGAGT GGCGCTGACC 
ACTTATTTCT CTGTGGATAA TTGCATGACA GACACCTACA GACTGAAGTA TCACCACCAG 
AGGCCATTGG TCCTCTCCAT GCCTGAGCCT AGAGGAGCTG GTAAGGACGG GAGGGATGCG 
GGACACTCCA GCCGCATGGA GAACCAGTCT GAGCGTGCCA AAAATAAGCC TGAGTCCGGC 
AATAGCAACA ATAAGCCCAC TGCTAAATGG CACCCTGTCA CTTCAAAAGG GCTAGATGAA 
CATGGATATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289672.1
CDS	105..2099
Translation	MDILPRLSFSQSPIGMGKHSYFNMNIYTQLSGCKKLDSVRDASAGSQRTNQGSHKTPMQSSTSRLKVLPESPSRFRESSRDLSHRGGGGQRRESDRSYRRSDSFQHKDMTYPRLSSPPENGPRFHCEQRPRTLPSRVSRTEHSSVHPRVSPQESRSNQGMASGVSRHADSSSLSHRGSTNPTERGKSKPSWRPVREVLNVDSVLSELERRQQQQQQQQRGSPRCVRRFAHERGLMKDKQKGLMTIYEDDSRLETESQSSQESQRQMMVTRSKGGANVAQRSDNWVIQRTESGYESSDRLSSGSTNPDSPGVENFTGKELRLSQDAQHLRDQGHNNQDDSKMEAARSPLYHGKHLAKIQGKNSEHLMKGSPSSRRKLRYSSCGTRKAGNTEKDPSGVDLPPEQQELSMQWHHSPESPLLRRLGKISSSECNSSDELTNPLSEQEDSAYRSSTSEVAPHESPGPNRSSYPWEESAIQSGPSIASHSKHAQRPIEIKTPPFRPRILQEPSQTASRASGRGAPSSSHSGAVHRRMEINGTCPSSSDVSSKSGSDQERGEQVSSEPEGWDKPSSSVSPGSGVALTTYFSVDNCMTDTYRLKYHHQRPLVLSMPEPRGAGKDGRDAGHSSRMENQSERAKNKPESGNSNNKPTAKWHPVTSKGLDEHGYL

Target ORF information:

RefSeq Version	XM_009291397.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291397.3

ORF Insert Sequence:

ATGGATATTC TCCCCCGGCT GTCATTCTCA CAGAGTCCTA TTGGAATGGG AAAGCATTCT 
TATTTTAATA TGAATATCTA CACTCAGCTG TCAGGCTGTA AGAAACTGGA CAGTGTGAGA 
GATGCGAGTG CTGGTTCCCA ACGAACAAAT CAGGGATCTC ACAAAACACC AATGCAGTCT 
TCAACCAGCC GGCTTAAAGT GCTTCCAGAA AGTCCAAGCA GATTCCGAGA GTCTTCCAGA 
GATTTGTCTC ACAGAGGAGG AGGAGGACAG AGGAGAGAGA GTGATAGAAG CTACCGAAGA 
TCAGACTCTT TCCAACACAA AGATATGACA TATCCACGCT TATCCTCTCC ACCTGAGAAT 
GGCCCAAGGT TTCATTGTGA GCAGAGGCCG CGAACTTTGC CCTCACGGGT CTCTCGAACT 
GAGCACTCTT CTGTTCATCC TCGTGTATCA CCCCAGGAGT CTCGCTCAAA CCAGGGGATG 
GCATCTGGAG TCAGTCGTCA CGCTGATTCA TCATCGCTTT CCCATCGTGG CTCCACAAAT 
CCCACTGAGC GTGGCAAGTC CAAGCCCTCA TGGAGACCAG TACGGGAGGT CCTCAATGTA 
GACAGTGTGC TGAGCGAACT AGAGCGACGA CAGCAGCAGC AACAGCAGCA ACAGCGTGGA 
AGCCCTCGAT GTGTGCGTCG ATTCGCCCAT GAGAGGGGGC TGATGAAGGA CAAGCAGAAG 
GGTCTGATGA CTATTTATGA AGATGACTCA CGGCTGGAGA CTGAAAGTCA AAGCTCCCAG 
GAGTCTCAAC GACAGATGAT GGTGACCAGG TCTAAAGGTG GAGCAAATGT AGCACAGCGA 
AGTGATAACT GGGTAATACA GAGGACAGAG TCAGGATATG AGAGCAGTGA TAGGCTGAGC 
AGCGGCTCTA CCAATCCTGA CTCACCTGGA GTTGAGAACT TTACCGGCAA AGAGCTGAGA 
CTCAGTCAAG ATGCACAGCA CCTCAGGGAT CAAGGCCATA ATAATCAAGA TGACTCAAAA 
ATGGAGGCGG CGCGTTCTCC ACTGTACCAC GGTAAACACT TGGCTAAAAT TCAGGGAAAG 
AACTCAGAAC ATCTCATGAA AGGCAGTCCA AGTTCAAGAA GGAAGCTTCG ATATTCATCC 
TGTGGTACCA GGAAAGCAGG CAACACAGAG AAAGACCCGT CAGGTGTGGA TCTTCCACCA 
GAGCAGCAGG AACTTTCCAT GCAATGGCAC CACAGCCCAG AAAGCCCATT ACTGCGCCGC 
CTGGGCAAGA TCAGCAGTTC AGAGTGTAAC AGTTCTGATG AACTCACTAA CCCTCTGTCA 
GAGCAGGAGG ACAGTGCTTA TCGTTCCAGC ACCAGTGAAG TCGCTCCACA TGAGTCACCG 
GGTCCCAATC GCTCTAGTTA CCCATGGGAA GAATCAGCCA TCCAATCAGG ACCCTCGATT 
GCAAGCCACT CCAAACACGC TCAGCGACCG ATCGAGATAA AGACTCCACC TTTCCGCCCC 
AGAATCTTAC AGGAACCATC TCAAACAGCT TCAAGAGCAT CTGGCCGTGG TGCTCCATCA 
TCTTCACACA GTGGCGCAGT ACACAGGCGA ATGGAGATCA ATGGCACGTG CCCCAGCTCG 
TCAGACGTGA GCTCAAAATC GGGATCAGAC CAGGAAAGAG GGGAGCAGGT GTCCAGCGAG 
CCCGAGGGTT GGGATAAGCC ATCTTCTTCT GTGTCTCCAG GATCTGGAGT GGCGCTGACC 
ACTTATTTCT CTGTGGATAA TTGCATGACA GACACCTACA GACTGAAGTA TCACCACCAG 
AGGCCATTGG TCCTCTCCAT GCCTGAGCCT AGAGGAGCTG GTAAGGACGG GAGGGATGCG 
GGACACTCCA GCCGCATGGA GAACCAGTCT GAGCGTGCCA AAAATAAGCC TGAGTCCGGC 
AATAGCAACA ATAAGCCCAC TGCTAAATGG CACCCTGTCA CTTCAAAAGG GCTAGATGAA 
CATGGATATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp53b ( XM_021471203.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326878.1 Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa144257
Clone ID Related Accession (Same CDS sequence)	XM_021471203.1
Accession Version	XM_021471203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein usp53b isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTGC TCATGTTTCA CAGAAGGAAG CTTCGATATT CATCCTGTGG TACCAGGAAA 
GCAGGCAACA CAGAGAAAGA CCCGTCAGGT GTGGATCTTC CACCAGAGCA GCAGGAACTT 
TCCATGCAAT GGCACCACAG CCCAGAAAGC CCATTACTGC GCCGCCTGGG CAAGATCAGC 
AGTTCAGAGT GTAACAGTTC TGATGAACTC ACTAACCCTC TGTCAGAGCA GGAGGACAGT 
GCTTATCGTT CCAGCACCAG TGAAGTCGCT CCACATGAGT CACCGGGTCC CAATCGCTCT 
AGTTACCCAT GGGAAGAATC AGCCATCCAA TCAGGACCCT CGATTGCAAG CCACTCCAAA 
CACGCTCAGC GACCGATCGA GATAAAGACT CCACCTTTCC GCCCCAGAAT CTTACAGGAA 
CCATCTCAAA CAGCTTCAAG AGCATCTGGC CGTGGTGCTC CATCATCTTC ACACAGTGGC 
GCAGTACACA GGCGAATGGA GATCAATGGC ACGTGCCCCA GCTCGTCAGA CGTGAGCTCA 
AAATCGGGAT CAGACCAGGA AAGAGGGGAG CAGGTGTCCA GCGAGCCCGA GGGTTGGGAT 
AAGCCATCTT CTTCTGTGTC TCCAGGATCT GGAGTGGCGC TGACCACTTA TTTCTCTGTG 
GATAATTGCA TGACAGACAC CTACAGACTG AAGTATCACC ACCAGAGGCC ATTGGTCCTC 
TCCATGCCTG AGCCTAGAGG AGCTGGTAAG GACGGGAGGG ATGCGGGACA CTCCAGCCGC 
ATGGAGAACC AGTCTGAGCG TGCCAAAAAT AAGCCTGAGT CCGGCAATAG CAACAATAAG 
CCCACTGCTA AATGGCACCC TGTCACTTCA AAAGGGCTAG ATGAACATGG ATATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326878.1
CDS	18..917
Translation	MRLLMFHRRKLRYSSCGTRKAGNTEKDPSGVDLPPEQQELSMQWHHSPESPLLRRLGKISSSECNSSDELTNPLSEQEDSAYRSSTSEVAPHESPGPNRSSYPWEESAIQSGPSIASHSKHAQRPIEIKTPPFRPRILQEPSQTASRASGRGAPSSSHSGAVHRRMEINGTCPSSSDVSSKSGSDQERGEQVSSEPEGWDKPSSSVSPGSGVALTTYFSVDNCMTDTYRLKYHHQRPLVLSMPEPRGAGKDGRDAGHSSRMENQSERAKNKPESGNSNNKPTAKWHPVTSKGLDEHGYL

Target ORF information:

RefSeq Version	XM_021471203.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471203.1

ORF Insert Sequence:

ATGAGATTGC TCATGTTTCA CAGAAGGAAG CTTCGATATT CATCCTGTGG TACCAGGAAA 
GCAGGCAACA CAGAGAAAGA CCCGTCAGGT GTGGATCTTC CACCAGAGCA GCAGGAACTT 
TCCATGCAAT GGCACCACAG CCCAGAAAGC CCATTACTGC GCCGCCTGGG CAAGATCAGC 
AGTTCAGAGT GTAACAGTTC TGATGAACTC ACTAACCCTC TGTCAGAGCA GGAGGACAGT 
GCTTATCGTT CCAGCACCAG TGAAGTCGCT CCACATGAGT CACCGGGTCC CAATCGCTCT 
AGTTACCCAT GGGAAGAATC AGCCATCCAA TCAGGACCCT CGATTGCAAG CCACTCCAAA 
CACGCTCAGC GACCGATCGA GATAAAGACT CCACCTTTCC GCCCCAGAAT CTTACAGGAA 
CCATCTCAAA CAGCTTCAAG AGCATCTGGC CGTGGTGCTC CATCATCTTC ACACAGTGGC 
GCAGTACACA GGCGAATGGA GATCAATGGC ACGTGCCCCA GCTCGTCAGA CGTGAGCTCA 
AAATCGGGAT CAGACCAGGA AAGAGGGGAG CAGGTGTCCA GCGAGCCCGA GGGTTGGGAT 
AAGCCATCTT CTTCTGTGTC TCCAGGATCT GGAGTGGCGC TGACCACTTA TTTCTCTGTG 
GATAATTGCA TGACAGACAC CTACAGACTG AAGTATCACC ACCAGAGGCC ATTGGTCCTC 
TCCATGCCTG AGCCTAGAGG AGCTGGTAAG GACGGGAGGG ATGCGGGACA CTCCAGCCGC 
ATGGAGAACC AGTCTGAGCG TGCCAAAAAT AAGCCTGAGT CCGGCAATAG CAACAATAAG 
CCCACTGCTA AATGGCACCC TGTCACTTCA AAAGGGCTAG ATGAACATGG ATATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp53b ( XM_021476531.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332206.1 Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa147405
Clone ID Related Accession (Same CDS sequence)	XM_021476531.1
Accession Version	XM_021476531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TCAAACTGTT TAAAAAGCCT GGAGGCAACA TGTCTGGCAC AGGTGCAAGG 
AGTGGGACGG GGCTGAGCAA GTCCTATCAG CCGGGCAGCA TGATCTCTCT CGCTTTGACC 
AAAGGACTTC TAAATGAGCC AGGCCAGAAC AGCTGCTTTC TCAACAGTGC GGTGCAGGTT 
CTTTGGCAGC TTGACATCTT CAGGAGGAGC CTACGACAGC TGTCTGGCCA TTTCTGCTTG 
GGTGATGCCT GTATTTTCTG CGCTTTAAAG AGCATCTTCT GTCAGTTCCA GCAGAGTCGA 
GAGCGGGCTC TGCCGTCAGA CACTCTACGT CACGCTCTGG CTGAGACCTT TAAAGATGAG 
CAGCGCTTCC AGCTGGGTCT CATGGATGAT GCAGCGGAGT GTTTTGAGAA CATTCTAGAA 
AGGATACACC TCCACCTTGT TTCTGACTCA GCTACAGAAA CATGTACTTC CAAATCATGC 
ATCACCCACC AGAAGTTTGC TATGACTCTC TATGAGCAGT TTGTTTGTCG TAGTTGTGGA 
GCATCATCAG ACCCTCTCCC TTTCACTGAG CTTGTGCACT ATGTTTCCTC TACAGCCCTC 
TGTCAACAAG TGGAGCGAAT CTTGGAGAAG ACAGACAGAC TGCGTTCAGA CATGTTTGGT 
GAACTGTTAC AGGCAGCCAA CACCATTGGA GATCTCCGCA ATTGCCCTCT GTTTTATAGA 
GTCACCGATG AAAATGCCAA GAAAAGAGAT CTTCACCTGG TGGGAATGAT CTGCTACTCT 
AGTCGACATT ACCTAGCATT CGCTTTTCAC AGCAAGTCTT CCAAATGGAT CTTTTTTGAT 
GATGCAACAG TGAAAGAGAT TGGCTCCAAG TGGAAAGATG TGGCATCTAA ATGTATTCGG 
GGTCACTTTC AGCCTCTTCT CTTGTTTTAT GCCAACCCTG ATGGTACTGC AGTGTCCAAT 
GAGGATGCCC CCCGACAGAC CACCATGTGG TCACGTTATA ACTCTCCTCT CAATGGAAAA 
GGGACAGACA CTCAGCTGTC AGGCTGTAAG AAACTGGACA GTGTGAGAGA TGCGAGTGCT 
GGTTCCCAAC GAACAAATCA GGGATCTCAC AAAACACCAA TGCAGTCTTC AACCAGCCGG 
CTTAAAGTGC TTCCAGAAAG TCCAAGCAGA TTCCGAGAGT CTTCCAGAGA TTTGTCTCAC 
AGAGGAGGAG GAGGACAGAG GAGAGAGAGT GATAGAAGCT ACCGAAGATC AGACTCTTTC 
CAACACAAAG ATATGACATA TCCACGCTTA TCCTCTCCAC CTGAGAATGG CCCAAGGTTT 
CATTGTGAGC AGAGGCCGCG AACTTTGCCC TCACGGGTCT CTCGAACTGA GCACTCTTCT 
GTTCATCCTC GTGTATCACC CCAGGAGTCT CGCTCAAACC AGGGGATGGC ATCTGGAGTC 
AGTCGTCACG CTGATTCATC ATCGCTTTCC CATCGTGGCT CCACAAATCC CACTGAGCGT 
GGCAAGTCCA AGCCCTCATG GAGACCAGTA CGGGAGGTCC TCAATGTAGA CAGTGTGCTG 
AGCGAACTAG AGCGACGACA GCAGCAGCAA CAGCAGCAAC AGCGTGGAAG CCCTCGATGT 
GTGCGTCGAT TCGCCCATGA GAGGGGGCTG ATGAAGGACA AGCAGAAGGG TCTGATGACT 
ATTTATGAAG ATGACTCACG GCTGGAGACT GAAAGTCAAA GCTCCCAGGA GTCTCAACGA 
CAGATGATGG TGACCAGGTC TAAAGGTGGA GCAAATGTAG CACAGCGAAG TGATAACTGG 
GTAATACAGA GGACAGAGTC AGGATATGAG AGCAGTGATA GGCTGAGCAG CGGCTCTACC 
AATCCTGACT CACCTGGAGT TGAGAACTTT ACCGGCAAAG AGCTGAGACT CAGTCAAGAT 
GCACAGCACC TCAGGGATCA AGGCCATAAT AATCAAGATG ACTCAAAAAT GGAGGCGGCG 
CGTTCTCCAC TGTACCACGG TAAACACTTG GCTAAAATTC AGGGAAAGAA CTCAGAACAT 
CTCATGAAAG GCAGTCCAAG TTCAAGAAGG AAGCTTCGAT ATTCATCCTG TGGTACCAGG 
AAAGCAGGCA ACACAGAGAA AGACCCGTCA GGTGTGGATC TTCCACCAGA GCAGCAGGAA 
CTTTCCATGC AATGGCACCA CAGCCCAGAA AGCCCATTAC TGCGCCGCCT GGGCAAGATC 
AGCAGTTCAG AGTGTAACAG TTCTGATGAA CTCACTAACC CTCTGTCAGA GCAGGAGGAC 
AGTGCTTATC GTTCCAGCAC CAGTGAAGTC GCTCCACATG AGTCACCGGG TCCCAATCGC 
TCTAGTTACC CATGGGAAGA ATCAGCCATC CAATCAGGAC CCTCGATTGC AAGCCACTCC 
AAACACGCTC AGCGACCGAT CGAGATAAAG ACTCCACCTT TCCGCCCCAG AATCTTACAG 
GAACCATCTC AAACAGCTTC AAGAGCATCT GGCCGTGGTG CTCCATCATC TTCACACAGT 
GGCGCAGTAC ACAGGCGAAT GGAGATCAAT GGCACGTGCC CCAGCTCGTC AGACGTGAGC 
TCAAAATCGG GATCAGACCA GGAAAGAGGG GAGCAGGTGT CCAGCGAGCC CGAGGGTTGG 
GATAAGCCAT CTTCTTCTGT GTCTCCAGGA TCTGGAGTGG CGCTGACCAC TTATTTCTCT 
GTGGATAATT GCATGACAGA CACCTACAGA CTGAAGTATC ACCACCAGAG GCCATTGGTC 
CTCTCCATGC CTGAGCCTAG AGGAGCTGGT AAGGACGGGA GGGATGCGGG ACACTCCAGC 
CGCATGGAGA ACCAGTCTGA GCGTGCCAAA AATAAGCCTG AGTCCGGCAA TAGCAACAAT 
AAGCCCACTG CTAAATGGCA CCCTGTCACT TCAAAAGGGC TAGATGAACA TGGATATTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332206.1
CDS	1027..3969
Translation	MAWVKLFKKPGGNMSGTGARSGTGLSKSYQPGSMISLALTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRQLSGHFCLGDACIFCALKSIFCQFQQSRERALPSDTLRHALAETFKDEQRFQLGLMDDAAECFENILERIHLHLVSDSATETCTSKSCITHQKFAMTLYEQFVCRSCGASSDPLPFTELVHYVSSTALCQQVERILEKTDRLRSDMFGELLQAANTIGDLRNCPLFYRVTDENAKKRDLHLVGMICYSSRHYLAFAFHSKSSKWIFFDDATVKEIGSKWKDVASKCIRGHFQPLLLFYANPDGTAVSNEDAPRQTTMWSRYNSPLNGKGTDTQLSGCKKLDSVRDASAGSQRTNQGSHKTPMQSSTSRLKVLPESPSRFRESSRDLSHRGGGGQRRESDRSYRRSDSFQHKDMTYPRLSSPPENGPRFHCEQRPRTLPSRVSRTEHSSVHPRVSPQESRSNQGMASGVSRHADSSSLSHRGSTNPTERGKSKPSWRPVREVLNVDSVLSELERRQQQQQQQQRGSPRCVRRFAHERGLMKDKQKGLMTIYEDDSRLETESQSSQESQRQMMVTRSKGGANVAQRSDNWVIQRTESGYESSDRLSSGSTNPDSPGVENFTGKELRLSQDAQHLRDQGHNNQDDSKMEAARSPLYHGKHLAKIQGKNSEHLMKGSPSSRRKLRYSSCGTRKAGNTEKDPSGVDLPPEQQELSMQWHHSPESPLLRRLGKISSSECNSSDELTNPLSEQEDSAYRSSTSEVAPHESPGPNRSSYPWEESAIQSGPSIASHSKHAQRPIEIKTPPFRPRILQEPSQTASRASGRGAPSSSHSGAVHRRMEINGTCPSSSDVSSKSGSDQERGEQVSSEPEGWDKPSSSVSPGSGVALTTYFSVDNCMTDTYRLKYHHQRPLVLSMPEPRGAGKDGRDAGHSSRMENQSERAKNKPESGNSNNKPTAKWHPVTSKGLDEHGYL

Target ORF information:

RefSeq Version	XM_021476531.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476531.1

ORF Insert Sequence:

ATGGCATGGG TCAAACTGTT TAAAAAGCCT GGAGGCAACA TGTCTGGCAC AGGTGCAAGG 
AGTGGGACGG GGCTGAGCAA GTCCTATCAG CCGGGCAGCA TGATCTCTCT CGCTTTGACC 
AAAGGACTTC TAAATGAGCC AGGCCAGAAC AGCTGCTTTC TCAACAGTGC GGTGCAGGTT 
CTTTGGCAGC TTGACATCTT CAGGAGGAGC CTACGACAGC TGTCTGGCCA TTTCTGCTTG 
GGTGATGCCT GTATTTTCTG CGCTTTAAAG AGCATCTTCT GTCAGTTCCA GCAGAGTCGA 
GAGCGGGCTC TGCCGTCAGA CACTCTACGT CACGCTCTGG CTGAGACCTT TAAAGATGAG 
CAGCGCTTCC AGCTGGGTCT CATGGATGAT GCAGCGGAGT GTTTTGAGAA CATTCTAGAA 
AGGATACACC TCCACCTTGT TTCTGACTCA GCTACAGAAA CATGTACTTC CAAATCATGC 
ATCACCCACC AGAAGTTTGC TATGACTCTC TATGAGCAGT TTGTTTGTCG TAGTTGTGGA 
GCATCATCAG ACCCTCTCCC TTTCACTGAG CTTGTGCACT ATGTTTCCTC TACAGCCCTC 
TGTCAACAAG TGGAGCGAAT CTTGGAGAAG ACAGACAGAC TGCGTTCAGA CATGTTTGGT 
GAACTGTTAC AGGCAGCCAA CACCATTGGA GATCTCCGCA ATTGCCCTCT GTTTTATAGA 
GTCACCGATG AAAATGCCAA GAAAAGAGAT CTTCACCTGG TGGGAATGAT CTGCTACTCT 
AGTCGACATT ACCTAGCATT CGCTTTTCAC AGCAAGTCTT CCAAATGGAT CTTTTTTGAT 
GATGCAACAG TGAAAGAGAT TGGCTCCAAG TGGAAAGATG TGGCATCTAA ATGTATTCGG 
GGTCACTTTC AGCCTCTTCT CTTGTTTTAT GCCAACCCTG ATGGTACTGC AGTGTCCAAT 
GAGGATGCCC CCCGACAGAC CACCATGTGG TCACGTTATA ACTCTCCTCT CAATGGAAAA 
GGGACAGACA CTCAGCTGTC AGGCTGTAAG AAACTGGACA GTGTGAGAGA TGCGAGTGCT 
GGTTCCCAAC GAACAAATCA GGGATCTCAC AAAACACCAA TGCAGTCTTC AACCAGCCGG 
CTTAAAGTGC TTCCAGAAAG TCCAAGCAGA TTCCGAGAGT CTTCCAGAGA TTTGTCTCAC 
AGAGGAGGAG GAGGACAGAG GAGAGAGAGT GATAGAAGCT ACCGAAGATC AGACTCTTTC 
CAACACAAAG ATATGACATA TCCACGCTTA TCCTCTCCAC CTGAGAATGG CCCAAGGTTT 
CATTGTGAGC AGAGGCCGCG AACTTTGCCC TCACGGGTCT CTCGAACTGA GCACTCTTCT 
GTTCATCCTC GTGTATCACC CCAGGAGTCT CGCTCAAACC AGGGGATGGC ATCTGGAGTC 
AGTCGTCACG CTGATTCATC ATCGCTTTCC CATCGTGGCT CCACAAATCC CACTGAGCGT 
GGCAAGTCCA AGCCCTCATG GAGACCAGTA CGGGAGGTCC TCAATGTAGA CAGTGTGCTG 
AGCGAACTAG AGCGACGACA GCAGCAGCAA CAGCAGCAAC AGCGTGGAAG CCCTCGATGT 
GTGCGTCGAT TCGCCCATGA GAGGGGGCTG ATGAAGGACA AGCAGAAGGG TCTGATGACT 
ATTTATGAAG ATGACTCACG GCTGGAGACT GAAAGTCAAA GCTCCCAGGA GTCTCAACGA 
CAGATGATGG TGACCAGGTC TAAAGGTGGA GCAAATGTAG CACAGCGAAG TGATAACTGG 
GTAATACAGA GGACAGAGTC AGGATATGAG AGCAGTGATA GGCTGAGCAG CGGCTCTACC 
AATCCTGACT CACCTGGAGT TGAGAACTTT ACCGGCAAAG AGCTGAGACT CAGTCAAGAT 
GCACAGCACC TCAGGGATCA AGGCCATAAT AATCAAGATG ACTCAAAAAT GGAGGCGGCG 
CGTTCTCCAC TGTACCACGG TAAACACTTG GCTAAAATTC AGGGAAAGAA CTCAGAACAT 
CTCATGAAAG GCAGTCCAAG TTCAAGAAGG AAGCTTCGAT ATTCATCCTG TGGTACCAGG 
AAAGCAGGCA ACACAGAGAA AGACCCGTCA GGTGTGGATC TTCCACCAGA GCAGCAGGAA 
CTTTCCATGC AATGGCACCA CAGCCCAGAA AGCCCATTAC TGCGCCGCCT GGGCAAGATC 
AGCAGTTCAG AGTGTAACAG TTCTGATGAA CTCACTAACC CTCTGTCAGA GCAGGAGGAC 
AGTGCTTATC GTTCCAGCAC CAGTGAAGTC GCTCCACATG AGTCACCGGG TCCCAATCGC 
TCTAGTTACC CATGGGAAGA ATCAGCCATC CAATCAGGAC CCTCGATTGC AAGCCACTCC 
AAACACGCTC AGCGACCGAT CGAGATAAAG ACTCCACCTT TCCGCCCCAG AATCTTACAG 
GAACCATCTC AAACAGCTTC AAGAGCATCT GGCCGTGGTG CTCCATCATC TTCACACAGT 
GGCGCAGTAC ACAGGCGAAT GGAGATCAAT GGCACGTGCC CCAGCTCGTC AGACGTGAGC 
TCAAAATCGG GATCAGACCA GGAAAGAGGG GAGCAGGTGT CCAGCGAGCC CGAGGGTTGG 
GATAAGCCAT CTTCTTCTGT GTCTCCAGGA TCTGGAGTGG CGCTGACCAC TTATTTCTCT 
GTGGATAATT GCATGACAGA CACCTACAGA CTGAAGTATC ACCACCAGAG GCCATTGGTC 
CTCTCCATGC CTGAGCCTAG AGGAGCTGGT AAGGACGGGA GGGATGCGGG ACACTCCAGC 
CGCATGGAGA ACCAGTCTGA GCGTGCCAAA AATAAGCCTG AGTCCGGCAA TAGCAACAAT 
AAGCCCACTG CTAAATGGCA CCCTGTCACT TCAAAAGGGC TAGATGAACA TGGATATTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp53b ( XM_005170957.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171014.1 Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05734
Clone ID Related Accession (Same CDS sequence)	XM_005170957.3 , XM_005170957.4
Accession Version	XM_005170957.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TCAAACTGTT TAAAAAGCCT GGAGGCAACA TGTCTGGCAC AGGTGCAAGG 
AGTGGGACGG GGCTGAGCAA GTCCTATCAG CCGGGCAGCA TGATCTCTCT CGCTTTGACC 
AAAGGACTTC TAAATGAGCC AGGCCAGAAC AGCTGCTTTC TCAACAGTGC GGTGCAGGTT 
CTTTGGCAGC TTGACATCTT CAGGAGGAGC CTACGACAGC TGTCTGGCCA TTTCTGCTTG 
GGTGATGCCT GTATTTTCTG CGCTTTAAAG AGCATCTTCT GTCAGTTCCA GCAGAGTCGA 
GAGCGGGCTC TGCCGTCAGA CACTCTACGT CACGCTCTGG CTGAGACCTT TAAAGATGAG 
CAGCGCTTCC AGCTGGGTCT CATGGATGAT GCAGCGGAGT GTTTTGAGAA CATTCTAGAA 
AGGATACACC TCCACCTTGT TTCTGACTCA GCTACAGAAA CATGTACTTC CAAATCATGC 
ATCACCCACC AGAAGTTTGC TATGACTCTC TATGAGCAGT TTGTTTGTCG TAGTTGTGGA 
GCATCATCAG ACCCTCTCCC TTTCACTGAG CTTGTGCACT ATGTTTCCTC TACAGCCCTC 
TGTCAACAAG TGGAGCGAAT CTTGGAGAAG ACAGACAGAC TGCGTTCAGA CATGTTTGGT 
GAACTGTTAC AGGCAGCCAA CACCATTGGA GATCTCCGCA ATTGCCCTAG TAACTGTGGG 
CAGAGTATCA AAATCCGCCG TGTGCTGATG AACTGCCCCG AGATTGTAAC CATTGGCTTT 
GTGTGGGACG CTGAACAGTC CGACCTCACT GAAGATGTCA TCCGCTGTCT GGGCCCAAAC 
CTCAATCTGT CAGGGCTGTT TTATAGAGTC ACCGATGAAA ATGCCAAGAA AAGAGATCTT 
CACCTGGTGG GAATGATCTG CTACTCTAGT CGACATTACC TAGCATTCGC TTTTCACAGC 
AAGTCTTCCA AATGGATCTT TTTTGATGAT GCAACAGTGA AAGAGATTGG CTCCAAGTGG 
AAAGATGTGG CATCTAAATG TATTCGGGGT CACTTTCAGC CTCTTCTCTT GTTTTATGCC 
AACCCTGATG GTACTGCAGT GTCCAATGAG GATGCCCCCC GACAGACCAC CATGTGGTCA 
CGTTATAACT CTCCTCTCAA TGGAAAAGGG ACAGACACTC AGCTGTCAGG CTGTAAGAAA 
CTGGACAGTG TGAGAGATGC GAGTGCTGGT TCCCAACGAA CAAATCAGGG ATCTCACAAA 
ACACCAATGC AGTCTTCAAC CAGCCGGCTT AAAGTGCTTC CAGAAAGTCC AAGCAGATTC 
CGAGAGTCTT CCAGAGATTT GTCTCACAGA GGAGGAGGAG GACAGAGGAG AGAGAGTGAT 
AGAAGCTACC GAAGATCAGA CTCTTTCCAA CACAAAGATA TGACATATCC ACGCTTATCC 
TCTCCACCTG AGAATGGCCC AAGGTTTCAT TGTGAGCAGA GGCCGCGAAC TTTGCCCTCA 
CGGGTCTCTC GAACTGAGCA CTCTTCTGTT CATCCTCGTG TATCACCCCA GGAGTCTCGC 
TCAAACCAGG GGATGGCATC TGGAGTCAGT CGTCACGCTG ATTCATCATC GCTTTCCCAT 
CGTGGCTCCA CAAATCCCAC TGAGCGTGGC AAGTCCAAGC CCTCATGGAG ACCAGTACGG 
GAGGTCCTCA ATGTAGACAG TGTGCTGAGC GAACTAGAGC GACGACAGCA GCAGCAACAG 
CAGCAACAGC GTGGAAGCCC TCGATGTGTG CGTCGATTCG CCCATGAGAG GGGGCTGATG 
AAGGACAAGC AGAAGGGTCT GATGACTATT TATGAAGATG ACTCACGGCT GGAGACTGAA 
AGTCAAAGCT CCCAGGAGTC TCAACGACAG ATGATGGTGA CCAGGTCTAA AGGTGGAGCA 
AATGTAGCAC AGCGAAGTGA TAACTGGGTA ATACAGAGGA CAGAGTCAGG ATATGAGAGC 
AGTGATAGGC TGAGCAGCGG CTCTACCAAT CCTGACTCAC CTGGAGTTGA GAACTTTACC 
GGCAAAGAGC TGAGACTCAG TCAAGATGCA CAGCACCTCA GGGATCAAGG CCATAATAAT 
CAAGATGACT CAAAAATGGA GGCGGCGCGT TCTCCACTGT ACCACGGTAA ACACTTGGCT 
AAAATTCAGG GAAAGAACTC AGAACATCTC ATGAAAGGCA GTCCAAGTTC AAGAAGGAAG 
CTTCGATATT CATCCTGTGG TACCAGGAAA GCAGGCAACA CAGAGAAAGA CCCGTCAGGT 
GTGGATCTTC CACCAGAGCA GCAGGAACTT TCCATGCAAT GGCACCACAG CCCAGAAAGC 
CCATTACTGC GCCGCCTGGG CAAGATCAGC AGTTCAGAGT GTAACAGTTC TGATGAACTC 
ACTAACCCTC TGTCAGAGCA GGAGGACAGT GCTTATCGTT CCAGCACCAG TGAAGTCGCT 
CCACATGAGT CACCGGGTCC CAATCGCTCT AGTTACCCAT GGGAAGAATC AGCCATCCAA 
TCAGGACCCT CGATTGCAAG CCACTCCAAA CACGCTCAGC GACCGATCGA GATAAAGACT 
CCACCTTTCC GCCCCAGAAT CTTACAGGAA CCATCTCAAA CAGCTTCAAG AGCATCTGGC 
CGTGGTGCTC CATCATCTTC ACACAGTGGC GCAGTACACA GGCGAATGGA GATCAATGGC 
ACGTGCCCCA GCTCGTCAGA CGTGAGCTCA AAATCGGGAT CAGACCAGGA AAGAGGGGAG 
CAGGTGTCCA GCGAGCCCGA GGGTTGGGAT AAGCCATCTT CTTCTGTGTC TCCAGGATCT 
GGAGTGGCGC TGACCACTTA TTTCTCTGTG GATAATTGCA TGACAGACAC CTACAGACTG 
AAGTATCACC ACCAGAGGCC ATTGGTCCTC TCCATGCCTG AGCCTAGAGG AGCTGGTAAG 
GACGGGAGGG ATGCGGGACA CTCCAGCCGC ATGGAGAACC AGTCTGAGCG TGCCAAAAAT 
AAGCCTGAGT CCGGCAATAG CAACAATAAG CCCACTGCTA AATGGCACCC TGTCACTTCA 
AAAGGGCTAG ATGAACATGG ATATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171014.1
CDS	940..4029
Translation	MAWVKLFKKPGGNMSGTGARSGTGLSKSYQPGSMISLALTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRQLSGHFCLGDACIFCALKSIFCQFQQSRERALPSDTLRHALAETFKDEQRFQLGLMDDAAECFENILERIHLHLVSDSATETCTSKSCITHQKFAMTLYEQFVCRSCGASSDPLPFTELVHYVSSTALCQQVERILEKTDRLRSDMFGELLQAANTIGDLRNCPSNCGQSIKIRRVLMNCPEIVTIGFVWDAEQSDLTEDVIRCLGPNLNLSGLFYRVTDENAKKRDLHLVGMICYSSRHYLAFAFHSKSSKWIFFDDATVKEIGSKWKDVASKCIRGHFQPLLLFYANPDGTAVSNEDAPRQTTMWSRYNSPLNGKGTDTQLSGCKKLDSVRDASAGSQRTNQGSHKTPMQSSTSRLKVLPESPSRFRESSRDLSHRGGGGQRRESDRSYRRSDSFQHKDMTYPRLSSPPENGPRFHCEQRPRTLPSRVSRTEHSSVHPRVSPQESRSNQGMASGVSRHADSSSLSHRGSTNPTERGKSKPSWRPVREVLNVDSVLSELERRQQQQQQQQRGSPRCVRRFAHERGLMKDKQKGLMTIYEDDSRLETESQSSQESQRQMMVTRSKGGANVAQRSDNWVIQRTESGYESSDRLSSGSTNPDSPGVENFTGKELRLSQDAQHLRDQGHNNQDDSKMEAARSPLYHGKHLAKIQGKNSEHLMKGSPSSRRKLRYSSCGTRKAGNTEKDPSGVDLPPEQQELSMQWHHSPESPLLRRLGKISSSECNSSDELTNPLSEQEDSAYRSSTSEVAPHESPGPNRSSYPWEESAIQSGPSIASHSKHAQRPIEIKTPPFRPRILQEPSQTASRASGRGAPSSSHSGAVHRRMEINGTCPSSSDVSSKSGSDQERGEQVSSEPEGWDKPSSSVSPGSGVALTTYFSVDNCMTDTYRLKYHHQRPLVLSMPEPRGAGKDGRDAGHSSRMENQSERAKNKPESGNSNNKPTAKWHPVTSKGLDEHGYL

Target ORF information:

RefSeq Version	XM_005170957.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170957.3

ORF Insert Sequence:

ATGGCATGGG TCAAACTGTT TAAAAAGCCT GGAGGCAACA TGTCTGGCAC AGGTGCAAGG 
AGTGGGACGG GGCTGAGCAA GTCCTATCAG CCGGGCAGCA TGATCTCTCT CGCTTTGACC 
AAAGGACTTC TAAATGAGCC AGGCCAGAAC AGCTGCTTTC TCAACAGTGC GGTGCAGGTT 
CTTTGGCAGC TTGACATCTT CAGGAGGAGC CTACGACAGC TGTCTGGCCA TTTCTGCTTG 
GGTGATGCCT GTATTTTCTG CGCTTTAAAG AGCATCTTCT GTCAGTTCCA GCAGAGTCGA 
GAGCGGGCTC TGCCGTCAGA CACTCTACGT CACGCTCTGG CTGAGACCTT TAAAGATGAG 
CAGCGCTTCC AGCTGGGTCT CATGGATGAT GCAGCGGAGT GTTTTGAGAA CATTCTAGAA 
AGGATACACC TCCACCTTGT TTCTGACTCA GCTACAGAAA CATGTACTTC CAAATCATGC 
ATCACCCACC AGAAGTTTGC TATGACTCTC TATGAGCAGT TTGTTTGTCG TAGTTGTGGA 
GCATCATCAG ACCCTCTCCC TTTCACTGAG CTTGTGCACT ATGTTTCCTC TACAGCCCTC 
TGTCAACAAG TGGAGCGAAT CTTGGAGAAG ACAGACAGAC TGCGTTCAGA CATGTTTGGT 
GAACTGTTAC AGGCAGCCAA CACCATTGGA GATCTCCGCA ATTGCCCTAG TAACTGTGGG 
CAGAGTATCA AAATCCGCCG TGTGCTGATG AACTGCCCCG AGATTGTAAC CATTGGCTTT 
GTGTGGGACG CTGAACAGTC CGACCTCACT GAAGATGTCA TCCGCTGTCT GGGCCCAAAC 
CTCAATCTGT CAGGGCTGTT TTATAGAGTC ACCGATGAAA ATGCCAAGAA AAGAGATCTT 
CACCTGGTGG GAATGATCTG CTACTCTAGT CGACATTACC TAGCATTCGC TTTTCACAGC 
AAGTCTTCCA AATGGATCTT TTTTGATGAT GCAACAGTGA AAGAGATTGG CTCCAAGTGG 
AAAGATGTGG CATCTAAATG TATTCGGGGT CACTTTCAGC CTCTTCTCTT GTTTTATGCC 
AACCCTGATG GTACTGCAGT GTCCAATGAG GATGCCCCCC GACAGACCAC CATGTGGTCA 
CGTTATAACT CTCCTCTCAA TGGAAAAGGG ACAGACACTC AGCTGTCAGG CTGTAAGAAA 
CTGGACAGTG TGAGAGATGC GAGTGCTGGT TCCCAACGAA CAAATCAGGG ATCTCACAAA 
ACACCAATGC AGTCTTCAAC CAGCCGGCTT AAAGTGCTTC CAGAAAGTCC AAGCAGATTC 
CGAGAGTCTT CCAGAGATTT GTCTCACAGA GGAGGAGGAG GACAGAGGAG AGAGAGTGAT 
AGAAGCTACC GAAGATCAGA CTCTTTCCAA CACAAAGATA TGACATATCC ACGCTTATCC 
TCTCCACCTG AGAATGGCCC AAGGTTTCAT TGTGAGCAGA GGCCGCGAAC TTTGCCCTCA 
CGGGTCTCTC GAACTGAGCA CTCTTCTGTT CATCCTCGTG TATCACCCCA GGAGTCTCGC 
TCAAACCAGG GGATGGCATC TGGAGTCAGT CGTCACGCTG ATTCATCATC GCTTTCCCAT 
CGTGGCTCCA CAAATCCCAC TGAGCGTGGC AAGTCCAAGC CCTCATGGAG ACCAGTACGG 
GAGGTCCTCA ATGTAGACAG TGTGCTGAGC GAACTAGAGC GACGACAGCA GCAGCAACAG 
CAGCAACAGC GTGGAAGCCC TCGATGTGTG CGTCGATTCG CCCATGAGAG GGGGCTGATG 
AAGGACAAGC AGAAGGGTCT GATGACTATT TATGAAGATG ACTCACGGCT GGAGACTGAA 
AGTCAAAGCT CCCAGGAGTC TCAACGACAG ATGATGGTGA CCAGGTCTAA AGGTGGAGCA 
AATGTAGCAC AGCGAAGTGA TAACTGGGTA ATACAGAGGA CAGAGTCAGG ATATGAGAGC 
AGTGATAGGC TGAGCAGCGG CTCTACCAAT CCTGACTCAC CTGGAGTTGA GAACTTTACC 
GGCAAAGAGC TGAGACTCAG TCAAGATGCA CAGCACCTCA GGGATCAAGG CCATAATAAT 
CAAGATGACT CAAAAATGGA GGCGGCGCGT TCTCCACTGT ACCACGGTAA ACACTTGGCT 
AAAATTCAGG GAAAGAACTC AGAACATCTC ATGAAAGGCA GTCCAAGTTC AAGAAGGAAG 
CTTCGATATT CATCCTGTGG TACCAGGAAA GCAGGCAACA CAGAGAAAGA CCCGTCAGGT 
GTGGATCTTC CACCAGAGCA GCAGGAACTT TCCATGCAAT GGCACCACAG CCCAGAAAGC 
CCATTACTGC GCCGCCTGGG CAAGATCAGC AGTTCAGAGT GTAACAGTTC TGATGAACTC 
ACTAACCCTC TGTCAGAGCA GGAGGACAGT GCTTATCGTT CCAGCACCAG TGAAGTCGCT 
CCACATGAGT CACCGGGTCC CAATCGCTCT AGTTACCCAT GGGAAGAATC AGCCATCCAA 
TCAGGACCCT CGATTGCAAG CCACTCCAAA CACGCTCAGC GACCGATCGA GATAAAGACT 
CCACCTTTCC GCCCCAGAAT CTTACAGGAA CCATCTCAAA CAGCTTCAAG AGCATCTGGC 
CGTGGTGCTC CATCATCTTC ACACAGTGGC GCAGTACACA GGCGAATGGA GATCAATGGC 
ACGTGCCCCA GCTCGTCAGA CGTGAGCTCA AAATCGGGAT CAGACCAGGA AAGAGGGGAG 
CAGGTGTCCA GCGAGCCCGA GGGTTGGGAT AAGCCATCTT CTTCTGTGTC TCCAGGATCT 
GGAGTGGCGC TGACCACTTA TTTCTCTGTG GATAATTGCA TGACAGACAC CTACAGACTG 
AAGTATCACC ACCAGAGGCC ATTGGTCCTC TCCATGCCTG AGCCTAGAGG AGCTGGTAAG 
GACGGGAGGG ATGCGGGACA CTCCAGCCGC ATGGAGAACC AGTCTGAGCG TGCCAAAAAT 
AAGCCTGAGT CCGGCAATAG CAACAATAAG CCCACTGCTA AATGGCACCC TGTCACTTCA 
AAAGGGCTAG ATGAACATGG ATATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp53b ( XM_005170957.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171014.1 Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X1, mRNA.

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CloneID	ODa05734
Clone ID Related Accession (Same CDS sequence)	XM_005170957.3 , XM_005170957.4
Accession Version	XM_005170957.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170957.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TCAAACTGTT TAAAAAGCCT GGAGGCAACA TGTCTGGCAC AGGTGCAAGG 
AGTGGGACGG GGCTGAGCAA GTCCTATCAG CCGGGCAGCA TGATCTCTCT CGCTTTGACC 
AAAGGACTTC TAAATGAGCC AGGCCAGAAC AGCTGCTTTC TCAACAGTGC GGTGCAGGTT 
CTTTGGCAGC TTGACATCTT CAGGAGGAGC CTACGACAGC TGTCTGGCCA TTTCTGCTTG 
GGTGATGCCT GTATTTTCTG CGCTTTAAAG AGCATCTTCT GTCAGTTCCA GCAGAGTCGA 
GAGCGGGCTC TGCCGTCAGA CACTCTACGT CACGCTCTGG CTGAGACCTT TAAAGATGAG 
CAGCGCTTCC AGCTGGGTCT CATGGATGAT GCAGCGGAGT GTTTTGAGAA CATTCTAGAA 
AGGATACACC TCCACCTTGT TTCTGACTCA GCTACAGAAA CATGTACTTC CAAATCATGC 
ATCACCCACC AGAAGTTTGC TATGACTCTC TATGAGCAGT TTGTTTGTCG TAGTTGTGGA 
GCATCATCAG ACCCTCTCCC TTTCACTGAG CTTGTGCACT ATGTTTCCTC TACAGCCCTC 
TGTCAACAAG TGGAGCGAAT CTTGGAGAAG ACAGACAGAC TGCGTTCAGA CATGTTTGGT 
GAACTGTTAC AGGCAGCCAA CACCATTGGA GATCTCCGCA ATTGCCCTAG TAACTGTGGG 
CAGAGTATCA AAATCCGCCG TGTGCTGATG AACTGCCCCG AGATTGTAAC CATTGGCTTT 
GTGTGGGACG CTGAACAGTC CGACCTCACT GAAGATGTCA TCCGCTGTCT GGGCCCAAAC 
CTCAATCTGT CAGGGCTGTT TTATAGAGTC ACCGATGAAA ATGCCAAGAA AAGAGATCTT 
CACCTGGTGG GAATGATCTG CTACTCTAGT CGACATTACC TAGCATTCGC TTTTCACAGC 
AAGTCTTCCA AATGGATCTT TTTTGATGAT GCAACAGTGA AAGAGATTGG CTCCAAGTGG 
AAAGATGTGG CATCTAAATG TATTCGGGGT CACTTTCAGC CTCTTCTCTT GTTTTATGCC 
AACCCTGATG GTACTGCAGT GTCCAATGAG GATGCCCCCC GACAGACCAC CATGTGGTCA 
CGTTATAACT CTCCTCTCAA TGGAAAAGGG ACAGACACTC AGCTGTCAGG CTGTAAGAAA 
CTGGACAGTG TGAGAGATGC GAGTGCTGGT TCCCAACGAA CAAATCAGGG ATCTCACAAA 
ACACCAATGC AGTCTTCAAC CAGCCGGCTT AAAGTGCTTC CAGAAAGTCC AAGCAGATTC 
CGAGAGTCTT CCAGAGATTT GTCTCACAGA GGAGGAGGAG GACAGAGGAG AGAGAGTGAT 
AGAAGCTACC GAAGATCAGA CTCTTTCCAA CACAAAGATA TGACATATCC ACGCTTATCC 
TCTCCACCTG AGAATGGCCC AAGGTTTCAT TGTGAGCAGA GGCCGCGAAC TTTGCCCTCA 
CGGGTCTCTC GAACTGAGCA CTCTTCTGTT CATCCTCGTG TATCACCCCA GGAGTCTCGC 
TCAAACCAGG GGATGGCATC TGGAGTCAGT CGTCACGCTG ATTCATCATC GCTTTCCCAT 
CGTGGCTCCA CAAATCCCAC TGAGCGTGGC AAGTCCAAGC CCTCATGGAG ACCAGTACGG 
GAGGTCCTCA ATGTAGACAG TGTGCTGAGC GAACTAGAGC GACGACAGCA GCAGCAACAG 
CAGCAACAGC GTGGAAGCCC TCGATGTGTG CGTCGATTCG CCCATGAGAG GGGGCTGATG 
AAGGACAAGC AGAAGGGTCT GATGACTATT TATGAAGATG ACTCACGGCT GGAGACTGAA 
AGTCAAAGCT CCCAGGAGTC TCAACGACAG ATGATGGTGA CCAGGTCTAA AGGTGGAGCA 
AATGTAGCAC AGCGAAGTGA TAACTGGGTA ATACAGAGGA CAGAGTCAGG ATATGAGAGC 
AGTGATAGGC TGAGCAGCGG CTCTACCAAT CCTGACTCAC CTGGAGTTGA GAACTTTACC 
GGCAAAGAGC TGAGACTCAG TCAAGATGCA CAGCACCTCA GGGATCAAGG CCATAATAAT 
CAAGATGACT CAAAAATGGA GGCGGCGCGT TCTCCACTGT ACCACGGTAA ACACTTGGCT 
AAAATTCAGG GAAAGAACTC AGAACATCTC ATGAAAGGCA GTCCAAGTTC AAGAAGGAAG 
CTTCGATATT CATCCTGTGG TACCAGGAAA GCAGGCAACA CAGAGAAAGA CCCGTCAGGT 
GTGGATCTTC CACCAGAGCA GCAGGAACTT TCCATGCAAT GGCACCACAG CCCAGAAAGC 
CCATTACTGC GCCGCCTGGG CAAGATCAGC AGTTCAGAGT GTAACAGTTC TGATGAACTC 
ACTAACCCTC TGTCAGAGCA GGAGGACAGT GCTTATCGTT CCAGCACCAG TGAAGTCGCT 
CCACATGAGT CACCGGGTCC CAATCGCTCT AGTTACCCAT GGGAAGAATC AGCCATCCAA 
TCAGGACCCT CGATTGCAAG CCACTCCAAA CACGCTCAGC GACCGATCGA GATAAAGACT 
CCACCTTTCC GCCCCAGAAT CTTACAGGAA CCATCTCAAA CAGCTTCAAG AGCATCTGGC 
CGTGGTGCTC CATCATCTTC ACACAGTGGC GCAGTACACA GGCGAATGGA GATCAATGGC 
ACGTGCCCCA GCTCGTCAGA CGTGAGCTCA AAATCGGGAT CAGACCAGGA AAGAGGGGAG 
CAGGTGTCCA GCGAGCCCGA GGGTTGGGAT AAGCCATCTT CTTCTGTGTC TCCAGGATCT 
GGAGTGGCGC TGACCACTTA TTTCTCTGTG GATAATTGCA TGACAGACAC CTACAGACTG 
AAGTATCACC ACCAGAGGCC ATTGGTCCTC TCCATGCCTG AGCCTAGAGG AGCTGGTAAG 
GACGGGAGGG ATGCGGGACA CTCCAGCCGC ATGGAGAACC AGTCTGAGCG TGCCAAAAAT 
AAGCCTGAGT CCGGCAATAG CAACAATAAG CCCACTGCTA AATGGCACCC TGTCACTTCA 
AAAGGGCTAG ATGAACATGG ATATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171014.1
CDS	1026..4115
Translation	MAWVKLFKKPGGNMSGTGARSGTGLSKSYQPGSMISLALTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRQLSGHFCLGDACIFCALKSIFCQFQQSRERALPSDTLRHALAETFKDEQRFQLGLMDDAAECFENILERIHLHLVSDSATETCTSKSCITHQKFAMTLYEQFVCRSCGASSDPLPFTELVHYVSSTALCQQVERILEKTDRLRSDMFGELLQAANTIGDLRNCPSNCGQSIKIRRVLMNCPEIVTIGFVWDAEQSDLTEDVIRCLGPNLNLSGLFYRVTDENAKKRDLHLVGMICYSSRHYLAFAFHSKSSKWIFFDDATVKEIGSKWKDVASKCIRGHFQPLLLFYANPDGTAVSNEDAPRQTTMWSRYNSPLNGKGTDTQLSGCKKLDSVRDASAGSQRTNQGSHKTPMQSSTSRLKVLPESPSRFRESSRDLSHRGGGGQRRESDRSYRRSDSFQHKDMTYPRLSSPPENGPRFHCEQRPRTLPSRVSRTEHSSVHPRVSPQESRSNQGMASGVSRHADSSSLSHRGSTNPTERGKSKPSWRPVREVLNVDSVLSELERRQQQQQQQQRGSPRCVRRFAHERGLMKDKQKGLMTIYEDDSRLETESQSSQESQRQMMVTRSKGGANVAQRSDNWVIQRTESGYESSDRLSSGSTNPDSPGVENFTGKELRLSQDAQHLRDQGHNNQDDSKMEAARSPLYHGKHLAKIQGKNSEHLMKGSPSSRRKLRYSSCGTRKAGNTEKDPSGVDLPPEQQELSMQWHHSPESPLLRRLGKISSSECNSSDELTNPLSEQEDSAYRSSTSEVAPHESPGPNRSSYPWEESAIQSGPSIASHSKHAQRPIEIKTPPFRPRILQEPSQTASRASGRGAPSSSHSGAVHRRMEINGTCPSSSDVSSKSGSDQERGEQVSSEPEGWDKPSSSVSPGSGVALTTYFSVDNCMTDTYRLKYHHQRPLVLSMPEPRGAGKDGRDAGHSSRMENQSERAKNKPESGNSNNKPTAKWHPVTSKGLDEHGYL

Target ORF information:

RefSeq Version	XM_005170957.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 53b (usp53b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170957.4

ORF Insert Sequence:

ATGGCATGGG TCAAACTGTT TAAAAAGCCT GGAGGCAACA TGTCTGGCAC AGGTGCAAGG 
AGTGGGACGG GGCTGAGCAA GTCCTATCAG CCGGGCAGCA TGATCTCTCT CGCTTTGACC 
AAAGGACTTC TAAATGAGCC AGGCCAGAAC AGCTGCTTTC TCAACAGTGC GGTGCAGGTT 
CTTTGGCAGC TTGACATCTT CAGGAGGAGC CTACGACAGC TGTCTGGCCA TTTCTGCTTG 
GGTGATGCCT GTATTTTCTG CGCTTTAAAG AGCATCTTCT GTCAGTTCCA GCAGAGTCGA 
GAGCGGGCTC TGCCGTCAGA CACTCTACGT CACGCTCTGG CTGAGACCTT TAAAGATGAG 
CAGCGCTTCC AGCTGGGTCT CATGGATGAT GCAGCGGAGT GTTTTGAGAA CATTCTAGAA 
AGGATACACC TCCACCTTGT TTCTGACTCA GCTACAGAAA CATGTACTTC CAAATCATGC 
ATCACCCACC AGAAGTTTGC TATGACTCTC TATGAGCAGT TTGTTTGTCG TAGTTGTGGA 
GCATCATCAG ACCCTCTCCC TTTCACTGAG CTTGTGCACT ATGTTTCCTC TACAGCCCTC 
TGTCAACAAG TGGAGCGAAT CTTGGAGAAG ACAGACAGAC TGCGTTCAGA CATGTTTGGT 
GAACTGTTAC AGGCAGCCAA CACCATTGGA GATCTCCGCA ATTGCCCTAG TAACTGTGGG 
CAGAGTATCA AAATCCGCCG TGTGCTGATG AACTGCCCCG AGATTGTAAC CATTGGCTTT 
GTGTGGGACG CTGAACAGTC CGACCTCACT GAAGATGTCA TCCGCTGTCT GGGCCCAAAC 
CTCAATCTGT CAGGGCTGTT TTATAGAGTC ACCGATGAAA ATGCCAAGAA AAGAGATCTT 
CACCTGGTGG GAATGATCTG CTACTCTAGT CGACATTACC TAGCATTCGC TTTTCACAGC 
AAGTCTTCCA AATGGATCTT TTTTGATGAT GCAACAGTGA AAGAGATTGG CTCCAAGTGG 
AAAGATGTGG CATCTAAATG TATTCGGGGT CACTTTCAGC CTCTTCTCTT GTTTTATGCC 
AACCCTGATG GTACTGCAGT GTCCAATGAG GATGCCCCCC GACAGACCAC CATGTGGTCA 
CGTTATAACT CTCCTCTCAA TGGAAAAGGG ACAGACACTC AGCTGTCAGG CTGTAAGAAA 
CTGGACAGTG TGAGAGATGC GAGTGCTGGT TCCCAACGAA CAAATCAGGG ATCTCACAAA 
ACACCAATGC AGTCTTCAAC CAGCCGGCTT AAAGTGCTTC CAGAAAGTCC AAGCAGATTC 
CGAGAGTCTT CCAGAGATTT GTCTCACAGA GGAGGAGGAG GACAGAGGAG AGAGAGTGAT 
AGAAGCTACC GAAGATCAGA CTCTTTCCAA CACAAAGATA TGACATATCC ACGCTTATCC 
TCTCCACCTG AGAATGGCCC AAGGTTTCAT TGTGAGCAGA GGCCGCGAAC TTTGCCCTCA 
CGGGTCTCTC GAACTGAGCA CTCTTCTGTT CATCCTCGTG TATCACCCCA GGAGTCTCGC 
TCAAACCAGG GGATGGCATC TGGAGTCAGT CGTCACGCTG ATTCATCATC GCTTTCCCAT 
CGTGGCTCCA CAAATCCCAC TGAGCGTGGC AAGTCCAAGC CCTCATGGAG ACCAGTACGG 
GAGGTCCTCA ATGTAGACAG TGTGCTGAGC GAACTAGAGC GACGACAGCA GCAGCAACAG 
CAGCAACAGC GTGGAAGCCC TCGATGTGTG CGTCGATTCG CCCATGAGAG GGGGCTGATG 
AAGGACAAGC AGAAGGGTCT GATGACTATT TATGAAGATG ACTCACGGCT GGAGACTGAA 
AGTCAAAGCT CCCAGGAGTC TCAACGACAG ATGATGGTGA CCAGGTCTAA AGGTGGAGCA 
AATGTAGCAC AGCGAAGTGA TAACTGGGTA ATACAGAGGA CAGAGTCAGG ATATGAGAGC 
AGTGATAGGC TGAGCAGCGG CTCTACCAAT CCTGACTCAC CTGGAGTTGA GAACTTTACC 
GGCAAAGAGC TGAGACTCAG TCAAGATGCA CAGCACCTCA GGGATCAAGG CCATAATAAT 
CAAGATGACT CAAAAATGGA GGCGGCGCGT TCTCCACTGT ACCACGGTAA ACACTTGGCT 
AAAATTCAGG GAAAGAACTC AGAACATCTC ATGAAAGGCA GTCCAAGTTC AAGAAGGAAG 
CTTCGATATT CATCCTGTGG TACCAGGAAA GCAGGCAACA CAGAGAAAGA CCCGTCAGGT 
GTGGATCTTC CACCAGAGCA GCAGGAACTT TCCATGCAAT GGCACCACAG CCCAGAAAGC 
CCATTACTGC GCCGCCTGGG CAAGATCAGC AGTTCAGAGT GTAACAGTTC TGATGAACTC 
ACTAACCCTC TGTCAGAGCA GGAGGACAGT GCTTATCGTT CCAGCACCAG TGAAGTCGCT 
CCACATGAGT CACCGGGTCC CAATCGCTCT AGTTACCCAT GGGAAGAATC AGCCATCCAA 
TCAGGACCCT CGATTGCAAG CCACTCCAAA CACGCTCAGC GACCGATCGA GATAAAGACT 
CCACCTTTCC GCCCCAGAAT CTTACAGGAA CCATCTCAAA CAGCTTCAAG AGCATCTGGC 
CGTGGTGCTC CATCATCTTC ACACAGTGGC GCAGTACACA GGCGAATGGA GATCAATGGC 
ACGTGCCCCA GCTCGTCAGA CGTGAGCTCA AAATCGGGAT CAGACCAGGA AAGAGGGGAG 
CAGGTGTCCA GCGAGCCCGA GGGTTGGGAT AAGCCATCTT CTTCTGTGTC TCCAGGATCT 
GGAGTGGCGC TGACCACTTA TTTCTCTGTG GATAATTGCA TGACAGACAC CTACAGACTG 
AAGTATCACC ACCAGAGGCC ATTGGTCCTC TCCATGCCTG AGCCTAGAGG AGCTGGTAAG 
GACGGGAGGG ATGCGGGACA CTCCAGCCGC ATGGAGAACC AGTCTGAGCG TGCCAAAAAT 
AAGCCTGAGT CCGGCAATAG CAACAATAAG CCCACTGCTA AATGGCACCC TGTCACTTCA 
AAAGGGCTAG ATGAACATGG ATATTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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usp53b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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