nkcc-1 cDNA ORF clone, Caenorhabditis elegans

The following nkcc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nkcc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa342737 NM_070304.1
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCa342530 NM_001268859.2
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCa342531 NM_001268860.2
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCa342736 NM_070303.2
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OCa342878 NM_001276883.2
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa342891 NM_001268862.2
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa342892 NM_001268861.2
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCa343518 NM_001083255.3
Latest version!
Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OCa343518 NM_001083255.2 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa342892 NM_001268861.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa342878 NM_001276883.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa342736 NM_070303.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa342530 NM_001268859.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCa342891 NM_001268862.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa342531 NM_001268860.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa342737
    Clone ID Related Accession (Same CDS sequence) NM_070304.1
    Accession Version NM_070304.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT AAAAAGCCGG AAACGGCTCA GTTGGCCAAG
    GTTATTGGGT TAAATCTCCT TAGTAAGGAG GACCAAGAGA GGCGAGATTT TGAGCGGAAA
    AAGCGGATTA ATCAACAAAA ACTGCAGGAA ATGCATAAAA TGCATAAGGA AACTTATAAT
    TTTCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_502705.1
    CDS1..789
    Translation

    Target ORF information:

    RefSeq Version NM_070304.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_070304.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT AAAAAGCCGG AAACGGCTCA GTTGGCCAAG
    GTTATTGGGT TAAATCTCCT TAGTAAGGAG GACCAAGAGA GGCGAGATTT TGAGCGGAAA
    AAGCGGATTA ATCAACAAAA ACTGCAGGAA ATGCATAAAA TGCATAAGGA AACTTATAAT
    TTTCGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342530
    Clone ID Related Accession (Same CDS sequence) NM_001268859.2 , NM_001268859.1
    Accession Version NM_001268859.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268859.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255788.1
    CDS1..2613
    Translation

    Target ORF information:

    RefSeq Version NM_001268859.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268859.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342530
    Clone ID Related Accession (Same CDS sequence) NM_001268859.2 , NM_001268859.1
    Accession Version NM_001268859.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255788.1
    CDS1..2613
    Translation

    Target ORF information:

    RefSeq Version NM_001268859.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342531
    Clone ID Related Accession (Same CDS sequence) NM_001268860.1 , NM_001268860.2
    Accession Version NM_001268860.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255789.1
    CDS1..1647
    Translation

    Target ORF information:

    RefSeq Version NM_001268860.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342531
    Clone ID Related Accession (Same CDS sequence) NM_001268860.1 , NM_001268860.2
    Accession Version NM_001268860.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268860.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255789.1
    CDS1..1647
    Translation

    Target ORF information:

    RefSeq Version NM_001268860.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342736
    Clone ID Related Accession (Same CDS sequence) NM_070303.1 , NM_070303.2
    Accession Version NM_070303.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT
    AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_502704.1
    CDS1..2919
    Translation

    Target ORF information:

    RefSeq Version NM_070303.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_070303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT
    AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342736
    Clone ID Related Accession (Same CDS sequence) NM_070303.1 , NM_070303.2
    Accession Version NM_070303.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_070303.1. COMPLETENESS: incomplete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT
    AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_502704.1
    CDS30..2948
    Translation

    Target ORF information:

    RefSeq Version NM_070303.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_070303.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT
    AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342878
    Clone ID Related Accession (Same CDS sequence) NM_001276883.1 , NM_001276883.2
    Accession Version NM_001276883.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
    GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG
    CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT
    GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT
    GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT
    AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC
    GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT
    ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT
    GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA
    GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC
    GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG
    AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG
    CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT
    GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG
    AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA
    TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT
    ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA
    TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG
    GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT
    CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA
    ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT
    TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA
    ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT
    GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT
    GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT
    TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC
    GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA
    TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT
    CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG
    AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC
    GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC
    TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263812.1
    CDS1..3567
    Translation

    Target ORF information:

    RefSeq Version NM_001276883.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
    GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG
    CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT
    GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT
    GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT
    AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC
    GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT
    ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT
    GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA
    GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC
    GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG
    AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG
    CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT
    GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG
    AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA
    TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT
    ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA
    TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG
    GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT
    CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA
    ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT
    TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA
    ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT
    GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT
    GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT
    TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC
    GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA
    TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT
    CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG
    AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC
    GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC
    TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342878
    Clone ID Related Accession (Same CDS sequence) NM_001276883.1 , NM_001276883.2
    Accession Version NM_001276883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001276883.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
    GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG
    CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT
    GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT
    GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT
    AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC
    GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT
    ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT
    GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA
    GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC
    GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG
    AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG
    CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT
    GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG
    AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA
    TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT
    ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA
    TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG
    GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT
    CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA
    ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT
    TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA
    ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT
    GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT
    GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT
    TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC
    GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA
    TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT
    CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG
    AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC
    GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC
    TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263812.1
    CDS67..3633
    Translation

    Target ORF information:

    RefSeq Version NM_001276883.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
    GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG
    CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT
    GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT
    GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT
    AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC
    GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT
    ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT
    GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA
    GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC
    GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG
    AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG
    CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT
    GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG
    AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA
    TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT
    ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA
    TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG
    GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT
    CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA
    ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT
    TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA
    ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT
    GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT
    GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT
    TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC
    GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA
    TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT
    CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG
    AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC
    GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC
    TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC
    ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG
    TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA
    AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC
    GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG
    GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG
    AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC
    TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC
    AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC
    CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC
    TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT
    ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT
    CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT
    CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG
    GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT
    TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC
    GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA
    GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG
    AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC
    AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC
    ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT
    CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG
    GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC
    AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG
    CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC
    AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT
    CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG
    TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA
    CAATCCGTCT TGACTTTCTA TTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342891
    Clone ID Related Accession (Same CDS sequence) NM_001268862.1 , NM_001268862.2
    Accession Version NM_001268862.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
    GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT
    CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA
    TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT
    GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA
    GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA
    CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG
    ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG
    TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC
    GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG
    AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC
    GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT
    CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG
    CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA
    GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT
    GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC
    GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT
    GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT
    GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC
    GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT
    CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT
    GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG
    GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA
    CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT
    ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT
    CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA
    ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT
    GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT
    TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA
    TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT
    GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC
    GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG
    ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG
    GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC
    GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG
    ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT
    AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA
    AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG
    GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG
    TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC
    ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT
    AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA
    GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC
    TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG
    ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT
    TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC
    ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT
    GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT
    GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA
    TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG
    AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT
    CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC
    TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT
    TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG
    CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC
    GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG
    TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT
    GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG
    CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC
    GTCTTGACTT TCTATTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255791.1
    CDS1..3561
    Translation

    Target ORF information:

    RefSeq Version NM_001268862.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
    GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT
    CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA
    TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT
    GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA
    GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA
    CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG
    ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG
    TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC
    GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG
    AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC
    GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT
    CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG
    CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA
    GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT
    GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC
    GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT
    GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT
    GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC
    GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT
    CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT
    GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG
    GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA
    CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT
    ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT
    CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA
    ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT
    GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT
    TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA
    TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT
    GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC
    GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG
    ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG
    GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC
    GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG
    ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT
    AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA
    AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG
    GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG
    TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC
    ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT
    AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA
    GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC
    TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG
    ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT
    TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC
    ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT
    GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT
    GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA
    TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG
    AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT
    CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC
    TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT
    TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG
    CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC
    GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG
    TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT
    GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG
    CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC
    GTCTTGACTT TCTATTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342891
    Clone ID Related Accession (Same CDS sequence) NM_001268862.1 , NM_001268862.2
    Accession Version NM_001268862.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268862.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
    GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT
    CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA
    TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT
    GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA
    GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA
    CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG
    ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG
    TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC
    GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG
    AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC
    GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT
    CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG
    CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA
    GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT
    GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC
    GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT
    GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT
    GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC
    GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT
    CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT
    GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG
    GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA
    CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT
    ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT
    CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA
    ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT
    GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT
    TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA
    TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT
    GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC
    GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG
    ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG
    GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC
    GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG
    ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT
    AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA
    AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG
    GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG
    TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC
    ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT
    AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA
    GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC
    TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG
    ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT
    TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC
    ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT
    GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT
    GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA
    TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG
    AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT
    CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC
    TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT
    TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG
    CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC
    GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG
    TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT
    GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG
    CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC
    GTCTTGACTT TCTATTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255791.1
    CDS56..3616
    Translation

    Target ORF information:

    RefSeq Version NM_001268862.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268862.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
    GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT
    CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA
    TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT
    GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA
    GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA
    CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG
    ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG
    TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC
    GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG
    AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC
    GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT
    CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG
    CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA
    GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT
    GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC
    GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT
    GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT
    GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC
    GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT
    CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT
    GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG
    GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA
    CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT
    ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT
    CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA
    ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT
    GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT
    TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA
    TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT
    GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC
    GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG
    ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG
    GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC
    GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG
    ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT
    AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA
    AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG
    GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG
    TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC
    ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT
    AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA
    GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC
    TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG
    ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT
    TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC
    ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT
    GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT
    GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA
    TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG
    AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT
    CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC
    TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT
    TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG
    CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC
    GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG
    TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT
    GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG
    CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC
    GTCTTGACTT TCTATTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342892
    Clone ID Related Accession (Same CDS sequence) NM_001268861.1 , NM_001268861.2
    Accession Version NM_001268861.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG
    ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG
    AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT
    GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG
    CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT
    CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG
    TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC
    TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA
    CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC
    TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG
    GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG
    GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT
    GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG
    GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA
    CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255790.1
    CDS1..3708
    Translation

    Target ORF information:

    RefSeq Version NM_001268861.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG
    ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG
    AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT
    GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG
    CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT
    CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG
    TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC
    TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA
    CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC
    TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG
    GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG
    GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT
    GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG
    GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA
    CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa342892
    Clone ID Related Accession (Same CDS sequence) NM_001268861.1 , NM_001268861.2
    Accession Version NM_001268861.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268861.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG
    ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG
    AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT
    GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG
    CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT
    CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG
    TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC
    TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA
    CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC
    TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG
    GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG
    GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT
    GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG
    GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA
    CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001255790.1
    CDS94..3801
    Translation

    Target ORF information:

    RefSeq Version NM_001268861.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001268861.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG
    ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG
    AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT
    GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG
    CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT
    CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG
    TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC
    TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA
    CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC
    TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG
    GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG
    GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT
    GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG
    GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA
    CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343518
    Clone ID Related Accession (Same CDS sequence) NM_001083255.2 , NM_001083255.3
    Accession Version NM_001083255.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001083255.1. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001076724.1
    CDS1..3633
    Translation

    Target ORF information:

    RefSeq Version NM_001083255.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083255.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa343518
    Clone ID Related Accession (Same CDS sequence) NM_001083255.2 , NM_001083255.3
    Accession Version NM_001083255.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Na-K-Cl Cotransporter homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001083255.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001076724.1
    CDS30..3662
    Translation

    Target ORF information:

    RefSeq Version NM_001083255.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083255.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
    GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT
    AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT
    GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT
    GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC
    GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA
    CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT
    GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG
    AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT
    ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA
    GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA
    GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT
    CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA
    GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC
    TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT
    TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT
    CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA
    ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA
    ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT
    CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC
    GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG
    ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG
    CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC
    ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC
    CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG
    ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT
    TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT
    CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA
    ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC
    AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA
    CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC
    CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC
    TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC
    TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC
    GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG
    CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG
    TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG
    GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT
    ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT
    CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT
    CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG
    TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA
    TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT
    GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA
    GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA
    AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG
    GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT
    GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA
    GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG
    AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG
    AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG
    CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA
    GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC
    CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA
    ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT
    ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC
    ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA
    CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT
    GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG
    TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA
    AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.