nkcc-1 gene cDNA ORF clone, Caenorhabditis elegans

mRNA	Protein	Name
NM_001083255.2	NP_001076724.1	Na-K-Cl Cotransporter homolog
NM_070303.1	NP_502704.1	Na-K-Cl Cotransporter homolog
NM_001083255.3	NP_001076724.1	Na-K-Cl Cotransporter homolog
NM_070304.1	NP_502705.1	Na-K-Cl Cotransporter homolog
NM_001268861.1	NP_001255790.1	Na-K-Cl Cotransporter homolog
NM_001276883.1	NP_001263812.1	Na-K-Cl Cotransporter homolog
NM_001268862.1	NP_001255791.1	Na-K-Cl Cotransporter homolog
NM_001268861.2	NP_001255790.1	Na-K-Cl Cotransporter homolog
NM_001276883.2	NP_001263812.1	Na-K-Cl Cotransporter homolog
NM_001268859.2	NP_001255788.1	Na-K-Cl Cotransporter homolog
NM_070303.2	NP_502704.1	Na-K-Cl Cotransporter homolog
NM_001268859.1	NP_001255788.1	Na-K-Cl Cotransporter homolog
NM_001268860.1	NP_001255789.1	Na-K-Cl Cotransporter homolog
NM_001268862.2	NP_001255791.1	Na-K-Cl Cotransporter homolog
NM_001268860.2	NP_001255789.1	Na-K-Cl Cotransporter homolog

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342737	NM_070304.1 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OCa342530	NM_001268859.2 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa342531	NM_001268860.2 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa342736	NM_070303.2 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa342878	NM_001276883.2 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa342891	NM_001268862.2 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa342892	NM_001268861.2 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa343518	NM_001083255.3 Latest version!	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa343518	NM_001083255.2	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa342892	NM_001268861.1	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa342878	NM_001276883.1	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa342736	NM_070303.1	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa342530	NM_001268859.1	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa342891	NM_001268862.1	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa342531	NM_001268860.1	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

nkcc-1 ( NM_070304.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502705.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Price & Availability↑

CloneID	OCa342737
Clone ID Related Accession (Same CDS sequence)	NM_070304.1
Accession Version	NM_070304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT AAAAAGCCGG AAACGGCTCA GTTGGCCAAG 
GTTATTGGGT TAAATCTCCT TAGTAAGGAG GACCAAGAGA GGCGAGATTT TGAGCGGAAA 
AAGCGGATTA ATCAACAAAA ACTGCAGGAA ATGCATAAAA TGCATAAGGA AACTTATAAT 
TTTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502705.1
CDS	1..789
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNKKPETAQLAKVIGLNLLSKEDQERRDFERKKRINQQKLQEMHKMHKETYNFR

Target ORF information:

RefSeq Version	NM_070304.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070304.1

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT AAAAAGCCGG AAACGGCTCA GTTGGCCAAG 
GTTATTGGGT TAAATCTCCT TAGTAAGGAG GACCAAGAGA GGCGAGATTT TGAGCGGAAA 
AAGCGGATTA ATCAACAAAA ACTGCAGGAA ATGCATAAAA TGCATAAGGA AACTTATAAT 
TTTCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268859.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255788.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Price & Availability↑

CloneID	OCa342530
Clone ID Related Accession (Same CDS sequence)	NM_001268859.2 , NM_001268859.1
Accession Version	NM_001268859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268859.1. COMPLETENESS: incomplete on the 5' end.

ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255788.1
CDS	1..2613
Translation	MYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268859.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268859.2

ORF Insert Sequence:

ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268859.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255788.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Price & Availability↑

CloneID	OCa342530
Clone ID Related Accession (Same CDS sequence)	NM_001268859.2 , NM_001268859.1
Accession Version	NM_001268859.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255788.1
CDS	1..2613
Translation	MYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268859.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268859.1

ORF Insert Sequence:

ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268860.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255789.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

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CloneID	OCa342531
Clone ID Related Accession (Same CDS sequence)	NM_001268860.1 , NM_001268860.2
Accession Version	NM_001268860.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255789.1
CDS	1..1647
Translation	MWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268860.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268860.1

ORF Insert Sequence:

ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268860.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255789.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

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CloneID	OCa342531
Clone ID Related Accession (Same CDS sequence)	NM_001268860.1 , NM_001268860.2
Accession Version	NM_001268860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268860.1. COMPLETENESS: incomplete on the 5' end.

ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255789.1
CDS	1..1647
Translation	MWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268860.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268860.2

ORF Insert Sequence:

ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_070303.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502704.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

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CloneID	OCa342736
Clone ID Related Accession (Same CDS sequence)	NM_070303.1 , NM_070303.2
Accession Version	NM_070303.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT 
AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502704.1
CDS	1..2919
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSRLSYRGSCRSVEMFQRINAFYINGSDKLS

Target ORF information:

RefSeq Version	NM_070303.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070303.1

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT 
AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_070303.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502704.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Price & Availability↑

CloneID	OCa342736
Clone ID Related Accession (Same CDS sequence)	NM_070303.1 , NM_070303.2
Accession Version	NM_070303.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_070303.1. COMPLETENESS: incomplete on the 3' end.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT 
AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502704.1
CDS	30..2948
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSRLSYRGSCRSVEMFQRINAFYINGSDKLS

Target ORF information:

RefSeq Version	NM_070303.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070303.2

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCTC GTCTATCATA TCGGGGTAGT TGCCGAAGTG TTGAAATGTT CCAGCGGATT 
AATGCTTTTT ATATTAATGG TTCGGACAAA TTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001276883.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001263812.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Price & Availability↑

CloneID	OCa342878
Clone ID Related Accession (Same CDS sequence)	NM_001276883.1 , NM_001276883.2
Accession Version	NM_001276883.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG 
CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT 
GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT 
GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT 
AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC 
GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT 
ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT 
GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA 
GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC 
GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG 
AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG 
CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT 
GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG 
AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA 
TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT 
ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA 
TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG 
GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT 
CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA 
ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT 
TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA 
ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT 
GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT 
GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT 
TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC 
GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA 
TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT 
CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG 
AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC 
GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC 
TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC 
ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263812.1
CDS	1..3567
Translation	MENEPPGVSNSRFEFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001276883.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276883.1

ORF Insert Sequence:

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG 
CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT 
GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT 
GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT 
AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC 
GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT 
ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT 
GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA 
GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC 
GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG 
AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG 
CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT 
GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG 
AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA 
TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT 
ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA 
TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG 
GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT 
CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA 
ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT 
TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA 
ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT 
GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT 
GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT 
TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC 
GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA 
TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT 
CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG 
AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC 
GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC 
TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC 
ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001276883.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001263812.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

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CloneID	OCa342878
Clone ID Related Accession (Same CDS sequence)	NM_001276883.1 , NM_001276883.2
Accession Version	NM_001276883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001276883.1.

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG 
CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT 
GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT 
GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT 
AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC 
GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT 
ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT 
GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA 
GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC 
GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG 
AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG 
CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT 
GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG 
AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA 
TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT 
ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA 
TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG 
GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT 
CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA 
ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT 
TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA 
ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT 
GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT 
GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT 
TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC 
GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA 
TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT 
CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG 
AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC 
GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC 
TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC 
ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263812.1
CDS	67..3633
Translation	MENEPPGVSNSRFEFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001276883.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276883.2

ORF Insert Sequence:

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGTTTCAGGT CAGTAAAACT 
GACGAGGACG AGAAAACCAG CGACGACAAT TTACGAAAAT TAAAAAGTGG CAGTGAGCCG 
CCTCCTCCGA CTCAAAATCA TGAAGCAGTG ACGCTTGTGA CGAGAAAAAT GAGTGCCACT 
GGAAGATTTA TGGTAACCTC TGACACAAAT CCGGGTCAAC TCGTCGCTGA AGTCGCTGAT 
GAACTTGGTA TTGATGTTCC GATAGATCCG CCGGCGGAAA AATCGCCGGC TTCACGTGCT 
AAAGGAGTAC ATTTCTCTGT TGGAGACAAG GATTCCGGTA GCATGTCGGA GGATGGCGAC 
GAGAAACGAA ATATAACACA CGAGAATCAG ACAACGTTCA ATATGAAAAG TTGGAGAAAT 
ATGAAGACGA TAGAGCATCC GCCGATTATC GATTTTTATC GGAATTCGAT TGATACAGAT 
GGGGTGTTCA ACAGGCCTTC GATGGCTCAA CTTATTCATG GAAAAGAGCA CAAAGAGCAA 
GATCTCGGAT TTGAGGAGTT GAATGCTCAA CACCACCATC TCGAAGATTC TCCAGTTCAC 
GAGACGAAAA ACGAGTATCG AATGGAGAAA ATGCACGCGC CTCCATCGCA AAATCGAGTG 
AAATTCGGTT GGATTCAGGG AGTATTCGTC AGATGTCTCC TGAATATCTT CGGAGTTATG 
CTTTATCTTC GTGTCAGTTG GGTTTCTGGT CAAGCTGGCG TTGGACTGGG TTCCTGTATT 
GTTCTGCTGG CTTCTGTCGT CACAACGATT ACGGCACTTT CCACGTGTGC AATTTGTACG 
AATGGAGATG TGAAGGGCGG TGGCGCGTAT TTTTTGATTT CCCGTTCACT TGGTCCTGAA 
TTCGGTGGCT CTATTGGAAT CATTTTCTCA ATTGCAAATG CAGTCGGTGC AGCAATGTAT 
ATCGTCGGTT TCGCCGAATC GTTTCGTGAT GTTCTCATCG ACTATAATAT TGGAACGATA 
TTTGATGGAG GTCTTTGGGA TGTTCGTGTT ATTGGATTCG TGACATGCGT GATTCTCATG 
GGAATTGTGT TCATTGGCTC CGAGTTTGAA TCGAAAATGC AGCTTGGACT CCTCGTGATT 
CTGCTCGTTT CCATCGCCAA CTACGTCTTC GGATCGTTCT TCCCGCCAAA TGAGATTGCA 
ATGAATCGAG GTGCAACTGG ATATTCACTG GATACTCTAC AATCCAACTT TATGCCACAT 
TTCACTGAAA ACAACACTTT CTTCTCAGTA TTCTCCGTCT ATTTCCCCGC TGCCACTGGA 
ATTATGGCCG GCGCGAACAT TAGTGGAGAT CTAGCGAATC CTCAACAAGC CATCCCACTT 
GGAACACTTC TGGCAATTCT TGTCACAACT ATCGTCTATT TGTTAACAGT TTGGATGACT 
GGAAGTACTG TAGTTGCGTT TTCAAATGGA ACAGAGCCGG CTGTCTTCAA TAATTCGTAT 
TTTGTTCCAC CAGATTGCAC GCCGGATTGT CCGTTTGGAT TGGTTTCTTA TTATCAGGTC 
GTCGAAATGT CATCCTTCTG GGGTCCTCTC ATCACTGCTG GAATCTTTGC CGCAACTCTA 
TCCTCTGCTC TCGCCAGTCT TGTCAGTGCT CCGAAGGTGT TCCAGGCGGT CTGCAAAGAT 
CGGCTTTTCC CGAGAATCGA CTATTTTGCG AAGACTTATG GAAAGAATGA GGAACCAAAG 
AGAGCATATG TTCTCGGATT TTTCTTGGCG ATGGGGATCG TTGCAATTGG TGACCTGAAC 
GTGATTGCTC CAATCATTTC GAACTTCTTC CTCGGCTCCT ACGCTCTGAT AAACTACGCC 
TGCTTCGATC AGTCCTTTGC CGATTCACCG GGCTTCCGAC CGGGCTTCAC CTACTACAAC 
ATGTGGATCT CCCTCGTCGG AGCACTTCTC TGTGTCTTTG TCATGTTCAT CATCGATTGG 
TTCTCGGCTC TCGTCACTTT CTTCTGTTTT GGAGCCATTT TCATGTATTT ATTGCATAGA 
AAACCCGATG TTAATTGGGG TTCATCGACA CAGGCACACA GTTACAAGAA CGCGTTGTCC 
GCTATGATTA AGCTTTCGAC TACCGAAGAG CACGTGAAGA ATTACAGGCC GCAGGTGTTG 
GTACTTAGTG GAAATCCGGC GAGTCGATCG TGTTTGGTGG ATTTCGCCAA TAATATTACG 
AAAGGAAGTA GTTTGTTGGT TTGTGGACAA GTCGTACCGT ATGAGCCATC TGATCGTGTC 
TATATGGTGA TGAAGCGTCT CGACGAGAAT ATCAACGTCT GGCTTCGAAA ACGTCATCTC 
AAAGCGTTCT ACCGTGCTGT CGCCAACTCG AATTTCCGGA AAGGTGCTCA ATCGTTGATC 
CAACTCACTG GAATCGCGAA GATGAAGCCG AATATAGTTT TGATGGGATT CAAATCGAAC 
TGGTATAAAG GAGGACCGAC GGAGACGAAT TTGAACGAGA TGAACGAATA TTTTGGAACT 
ATTCAAGACG TTTTCGACTG GAATATGGCA ATGTGTGTTC TACGAAATGG CCAAGTCGGT 
CTCGACTTTT CTGAAGCAAT GAGAAATTTG AATCTTATTG AACCGAATCG ATTAAATGTT 
CCACCGATTG AGGAGAAGGA GAAAGAAAAA GAGAAAACGA ATGAAAACTC GCCGGAAACG 
GTTCATTTAA TCGAAAAAGA CGAGACAGCG AGAACTGACA AGTCAGGAGA CGATGGTTCT 
TCATCCATCT CAATCAACGA AACATACGTA TCAGATGAGA ATCAAGAAGA CGCAGATGGC 
GATGATGATA ATGATAATGA TGGAGAAGAA TCTGGAGCTG ATGATGAGCA TCAGAAGGAA 
GATGATGTCG AGTTGGGCGT TATTGATGAG CAGGAAAAGC GACATTTCTC GTTGAGGCGG 
AGAGGATCTC GACGTCATAC TGTTGAGCAG AAGGCGCTTC TCTCGTCAAT TCAGCGCTTC 
AATCGGAAGG TCAAGAAGGG AACCATCGAC GTTTGGTGGT TGTATGATGA CGGAGGTCTC 
ACTCTTCTCA TCCCACACCT TCTCGCCATA CCGAAAAGCT ATCTGGAAGG TGCCCGCCTT 
CGGATCTTCA CCATCTCCAC GTCATCCCGA ACGATGGAAC AAGAGCAACG TGGAATGGCG 
GCTCTTTTGT CGAAATTCCG AATCGATTAT TCGGATGTCT ACGTCATCGC TGATATTGGC 
AAGAAGCCGC GTCAGGAGAC GATGAACACG TGGCAATCGG TAATCGATCC GTTCATCGCG 
CAGGACGGAA GCTGTCCGAT TGGAATGACG ACGAAGTCGG AGTTGAGTGC TCAACGAGAC 
AAGACGTATC GGCAGTTGAG AGCTGGAGAG CTTCTGAATG AGCATTCGAT AAATGCAGAT 
CTTATTGTGA TGACTCTACC AGTACCACGC AAAGGAATGG TTAGCTCATC ATTGTACATG 
TCGTGGCTGG AAGTGATGAC TCGAAACTTG CCTCCAGTAC TCCTCGTTCG CGGAAATCAA 
CAATCCGTCT TGACTTTCTA TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268862.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255791.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

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CloneID	OCa342891
Clone ID Related Accession (Same CDS sequence)	NM_001268862.1 , NM_001268862.2
Accession Version	NM_001268862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT 
CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA 
TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT 
GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA 
GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA 
CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG 
ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG 
TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC 
GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG 
AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC 
GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT 
CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG 
CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA 
GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT 
GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC 
GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT 
GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT 
GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC 
GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT 
CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT 
GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG 
GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA 
CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT 
ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT 
CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA 
ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT 
GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT 
TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA 
TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT 
GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC 
GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG 
ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG 
GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC 
GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG 
ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT 
AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA 
AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG 
GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG 
TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC 
ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT 
AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA 
GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC 
TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG 
ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT 
TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC 
ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT 
GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT 
GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA 
TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG 
AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT 
CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC 
TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT 
TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG 
CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC 
GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG 
TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT 
GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG 
CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC 
GTCTTGACTT TCTATTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255791.1
CDS	1..3561
Translation	MENEPPGVSNSRFEVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268862.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268862.1

ORF Insert Sequence:

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT 
CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA 
TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT 
GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA 
GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA 
CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG 
ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG 
TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC 
GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG 
AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC 
GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT 
CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG 
CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA 
GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT 
GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC 
GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT 
GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT 
GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC 
GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT 
CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT 
GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG 
GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA 
CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT 
ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT 
CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA 
ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT 
GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT 
TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA 
TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT 
GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC 
GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG 
ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG 
GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC 
GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG 
ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT 
AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA 
AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG 
GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG 
TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC 
ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT 
AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA 
GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC 
TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG 
ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT 
TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC 
ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT 
GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT 
GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA 
TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG 
AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT 
CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC 
TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT 
TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG 
CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC 
GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG 
TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT 
GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG 
CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC 
GTCTTGACTT TCTATTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268862.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255791.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

Price & Availability↑

CloneID	OCa342891
Clone ID Related Accession (Same CDS sequence)	NM_001268862.1 , NM_001268862.2
Accession Version	NM_001268862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268862.1.

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT 
CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA 
TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT 
GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA 
GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA 
CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG 
ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG 
TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC 
GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG 
AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC 
GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT 
CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG 
CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA 
GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT 
GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC 
GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT 
GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT 
GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC 
GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT 
CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT 
GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG 
GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA 
CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT 
ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT 
CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA 
ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT 
GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT 
TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA 
TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT 
GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC 
GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG 
ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG 
GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC 
GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG 
ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT 
AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA 
AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG 
GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG 
TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC 
ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT 
AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA 
GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC 
TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG 
ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT 
TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC 
ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT 
GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT 
GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA 
TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG 
AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT 
CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC 
TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT 
TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG 
CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC 
GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG 
TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT 
GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG 
CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC 
GTCTTGACTT TCTATTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255791.1
CDS	56..3616
Translation	MENEPPGVSNSRFEVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268862.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268862.2

ORF Insert Sequence:

ATGGAAAACG AACCTCCCGG GGTTTCGAAC AGTCGGTTTG AGGTCAGTAA AACTGACGAG 
GACGAGAAAA CCAGCGACGA CAATTTACGA AAATTAAAAA GTGGCAGTGA GCCGCCTCCT 
CCGACTCAAA ATCATGAAGC AGTGACGCTT GTGACGAGAA AAATGAGTGC CACTGGAAGA 
TTTATGGTAA CCTCTGACAC AAATCCGGGT CAACTCGTCG CTGAAGTCGC TGATGAACTT 
GGTATTGATG TTCCGATAGA TCCGCCGGCG GAAAAATCGC CGGCTTCACG TGCTAAAGGA 
GTACATTTCT CTGTTGGAGA CAAGGATTCC GGTAGCATGT CGGAGGATGG CGACGAGAAA 
CGAAATATAA CACACGAGAA TCAGACAACG TTCAATATGA AAAGTTGGAG AAATATGAAG 
ACGATAGAGC ATCCGCCGAT TATCGATTTT TATCGGAATT CGATTGATAC AGATGGGGTG 
TTCAACAGGC CTTCGATGGC TCAACTTATT CATGGAAAAG AGCACAAAGA GCAAGATCTC 
GGATTTGAGG AGTTGAATGC TCAACACCAC CATCTCGAAG ATTCTCCAGT TCACGAGACG 
AAAAACGAGT ATCGAATGGA GAAAATGCAC GCGCCTCCAT CGCAAAATCG AGTGAAATTC 
GGTTGGATTC AGGGAGTATT CGTCAGATGT CTCCTGAATA TCTTCGGAGT TATGCTTTAT 
CTTCGTGTCA GTTGGGTTTC TGGTCAAGCT GGCGTTGGAC TGGGTTCCTG TATTGTTCTG 
CTGGCTTCTG TCGTCACAAC GATTACGGCA CTTTCCACGT GTGCAATTTG TACGAATGGA 
GATGTGAAGG GCGGTGGCGC GTATTTTTTG ATTTCCCGTT CACTTGGTCC TGAATTCGGT 
GGCTCTATTG GAATCATTTT CTCAATTGCA AATGCAGTCG GTGCAGCAAT GTATATCGTC 
GGTTTCGCCG AATCGTTTCG TGATGTTCTC ATCGACTATA ATATTGGAAC GATATTTGAT 
GGAGGTCTTT GGGATGTTCG TGTTATTGGA TTCGTGACAT GCGTGATTCT CATGGGAATT 
GTGTTCATTG GCTCCGAGTT TGAATCGAAA ATGCAGCTTG GACTCCTCGT GATTCTGCTC 
GTTTCCATCG CCAACTACGT CTTCGGATCG TTCTTCCCGC CAAATGAGAT TGCAATGAAT 
CGAGGTGCAA CTGGATATTC ACTGGATACT CTACAATCCA ACTTTATGCC ACATTTCACT 
GAAAACAACA CTTTCTTCTC AGTATTCTCC GTCTATTTCC CCGCTGCCAC TGGAATTATG 
GCCGGCGCGA ACATTAGTGG AGATCTAGCG AATCCTCAAC AAGCCATCCC ACTTGGAACA 
CTTCTGGCAA TTCTTGTCAC AACTATCGTC TATTTGTTAA CAGTTTGGAT GACTGGAAGT 
ACTGTAGTTG CGTTTTCAAA TGGAACAGAG CCGGCTGTCT TCAATAATTC GTATTTTGTT 
CCACCAGATT GCACGCCGGA TTGTCCGTTT GGATTGGTTT CTTATTATCA GGTCGTCGAA 
ATGTCATCCT TCTGGGGTCC TCTCATCACT GCTGGAATCT TTGCCGCAAC TCTATCCTCT 
GCTCTCGCCA GTCTTGTCAG TGCTCCGAAG GTGTTCCAGG CGGTCTGCAA AGATCGGCTT 
TTCCCGAGAA TCGACTATTT TGCGAAGACT TATGGAAAGA ATGAGGAACC AAAGAGAGCA 
TATGTTCTCG GATTTTTCTT GGCGATGGGG ATCGTTGCAA TTGGTGACCT GAACGTGATT 
GCTCCAATCA TTTCGAACTT CTTCCTCGGC TCCTACGCTC TGATAAACTA CGCCTGCTTC 
GATCAGTCCT TTGCCGATTC ACCGGGCTTC CGACCGGGCT TCACCTACTA CAACATGTGG 
ATCTCCCTCG TCGGAGCACT TCTCTGTGTC TTTGTCATGT TCATCATCGA TTGGTTCTCG 
GCTCTCGTCA CTTTCTTCTG TTTTGGAGCC ATTTTCATGT ATTTATTGCA TAGAAAACCC 
GATGTTAATT GGGGTTCATC GACACAGGCA CACAGTTACA AGAACGCGTT GTCCGCTATG 
ATTAAGCTTT CGACTACCGA AGAGCACGTG AAGAATTACA GGCCGCAGGT GTTGGTACTT 
AGTGGAAATC CGGCGAGTCG ATCGTGTTTG GTGGATTTCG CCAATAATAT TACGAAAGGA 
AGTAGTTTGT TGGTTTGTGG ACAAGTCGTA CCGTATGAGC CATCTGATCG TGTCTATATG 
GTGATGAAGC GTCTCGACGA GAATATCAAC GTCTGGCTTC GAAAACGTCA TCTCAAAGCG 
TTCTACCGTG CTGTCGCCAA CTCGAATTTC CGGAAAGGTG CTCAATCGTT GATCCAACTC 
ACTGGAATCG CGAAGATGAA GCCGAATATA GTTTTGATGG GATTCAAATC GAACTGGTAT 
AAAGGAGGAC CGACGGAGAC GAATTTGAAC GAGATGAACG AATATTTTGG AACTATTCAA 
GACGTTTTCG ACTGGAATAT GGCAATGTGT GTTCTACGAA ATGGCCAAGT CGGTCTCGAC 
TTTTCTGAAG CAATGAGAAA TTTGAATCTT ATTGAACCGA ATCGATTAAA TGTTCCACCG 
ATTGAGGAGA AGGAGAAAGA AAAAGAGAAA ACGAATGAAA ACTCGCCGGA AACGGTTCAT 
TTAATCGAAA AAGACGAGAC AGCGAGAACT GACAAGTCAG GAGACGATGG TTCTTCATCC 
ATCTCAATCA ACGAAACATA CGTATCAGAT GAGAATCAAG AAGACGCAGA TGGCGATGAT 
GATAATGATA ATGATGGAGA AGAATCTGGA GCTGATGATG AGCATCAGAA GGAAGATGAT 
GTCGAGTTGG GCGTTATTGA TGAGCAGGAA AAGCGACATT TCTCGTTGAG GCGGAGAGGA 
TCTCGACGTC ATACTGTTGA GCAGAAGGCG CTTCTCTCGT CAATTCAGCG CTTCAATCGG 
AAGGTCAAGA AGGGAACCAT CGACGTTTGG TGGTTGTATG ATGACGGAGG TCTCACTCTT 
CTCATCCCAC ACCTTCTCGC CATACCGAAA AGCTATCTGG AAGGTGCCCG CCTTCGGATC 
TTCACCATCT CCACGTCATC CCGAACGATG GAACAAGAGC AACGTGGAAT GGCGGCTCTT 
TTGTCGAAAT TCCGAATCGA TTATTCGGAT GTCTACGTCA TCGCTGATAT TGGCAAGAAG 
CCGCGTCAGG AGACGATGAA CACGTGGCAA TCGGTAATCG ATCCGTTCAT CGCGCAGGAC 
GGAAGCTGTC CGATTGGAAT GACGACGAAG TCGGAGTTGA GTGCTCAACG AGACAAGACG 
TATCGGCAGT TGAGAGCTGG AGAGCTTCTG AATGAGCATT CGATAAATGC AGATCTTATT 
GTGATGACTC TACCAGTACC ACGCAAAGGA ATGGTTAGCT CATCATTGTA CATGTCGTGG 
CTGGAAGTGA TGACTCGAAA CTTGCCTCCA GTACTCCTCG TTCGCGGAAA TCAACAATCC 
GTCTTGACTT TCTATTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268861.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255790.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

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CloneID	OCa342892
Clone ID Related Accession (Same CDS sequence)	NM_001268861.1 , NM_001268861.2
Accession Version	NM_001268861.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG 
ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG 
AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT 
GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG 
CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT 
CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG 
TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC 
TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA 
CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC 
TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG 
GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG 
GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT 
GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG 
GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA 
CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255790.1
CDS	1..3708
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSARLSYRGSCRSVEMFQRINAFYINASISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268861.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268861.1

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG 
ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG 
AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT 
GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG 
CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT 
CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG 
TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC 
TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA 
CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC 
TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG 
GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG 
GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT 
GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG 
GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA 
CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001268861.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255790.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

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CloneID	OCa342892
Clone ID Related Accession (Same CDS sequence)	NM_001268861.1 , NM_001268861.2
Accession Version	NM_001268861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001268861.1.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG 
ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG 
AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT 
GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG 
CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT 
CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG 
TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC 
TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA 
CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC 
TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG 
GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG 
GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT 
GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG 
GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA 
CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255790.1
CDS	94..3801
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSARLSYRGSCRSVEMFQRINAFYINASISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001268861.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268861.2

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAG CTCGTCTATC ATATCGGGGT AGTTGCCGAA GTGTTGAAAT GTTCCAGCGG 
ATTAATGCTT TTTATATTAA TGCATCCATC TCAATCAACG AAACATACGT ATCAGATGAG 
AATCAAGAAG ACGCAGATGG CGATGATGAT AATGATAATG ATGGAGAAGA ATCTGGAGCT 
GATGATGAGC ATCAGAAGGA AGATGATGTC GAGTTGGGCG TTATTGATGA GCAGGAAAAG 
CGACATTTCT CGTTGAGGCG GAGAGGATCT CGACGTCATA CTGTTGAGCA GAAGGCGCTT 
CTCTCGTCAA TTCAGCGCTT CAATCGGAAG GTCAAGAAGG GAACCATCGA CGTTTGGTGG 
TTGTATGATG ACGGAGGTCT CACTCTTCTC ATCCCACACC TTCTCGCCAT ACCGAAAAGC 
TATCTGGAAG GTGCCCGCCT TCGGATCTTC ACCATCTCCA CGTCATCCCG AACGATGGAA 
CAAGAGCAAC GTGGAATGGC GGCTCTTTTG TCGAAATTCC GAATCGATTA TTCGGATGTC 
TACGTCATCG CTGATATTGG CAAGAAGCCG CGTCAGGAGA CGATGAACAC GTGGCAATCG 
GTAATCGATC CGTTCATCGC GCAGGACGGA AGCTGTCCGA TTGGAATGAC GACGAAGTCG 
GAGTTGAGTG CTCAACGAGA CAAGACGTAT CGGCAGTTGA GAGCTGGAGA GCTTCTGAAT 
GAGCATTCGA TAAATGCAGA TCTTATTGTG ATGACTCTAC CAGTACCACG CAAAGGAATG 
GTTAGCTCAT CATTGTACAT GTCGTGGCTG GAAGTGATGA CTCGAAACTT GCCTCCAGTA 
CTCCTCGTTC GCGGAAATCA ACAATCCGTC TTGACTTTCT ATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001083255.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076724.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

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CloneID	OCa343518
Clone ID Related Accession (Same CDS sequence)	NM_001083255.2 , NM_001083255.3
Accession Version	NM_001083255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_001083255.1. COMPLETENESS: incomplete on both ends.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076724.1
CDS	1..3633
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001083255.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083255.2

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkcc-1 ( NM_001083255.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001076724.1 Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

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CloneID	OCa343518
Clone ID Related Accession (Same CDS sequence)	NM_001083255.2 , NM_001083255.3
Accession Version	NM_001083255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Na-K-Cl Cotransporter homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001083255.2.

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076724.1
CDS	30..3662
Translation	MNGNGENGTDEKQKLLSVVGVVSEEVVEMNRVSSARFQVSKTDEDEKTSDDNLRKLKSGSEPPPPTQNHEAVTLVTRKMSATGRFMVTSDTNPGQLVAEVADELGIDVPIDPPAEKSPASRAKGVHFSVGDKDSGSMSEDGDEKRNITHENQTTFNMKSWRNMKTIEHPPIIDFYRNSIDTDGVFNRPSMAQLIHGKEHKEQDLGFEELNAQHHHLEDSPVHETKNEYRMEKMHAPPSQNRVKFGWIQGVFVRCLLNIFGVMLYLRVSWVSGQAGVGLGSCIVLLASVVTTITALSTCAICTNGDVKGGGAYFLISRSLGPEFGGSIGIIFSIANAVGAAMYIVGFAESFRDVLIDYNIGTIFDGGLWDVRVIGFVTCVILMGIVFIGSEFESKMQLGLLVILLVSIANYVFGSFFPPNEIAMNRGATGYSLDTLQSNFMPHFTENNTFFSVFSVYFPAATGIMAGANISGDLANPQQAIPLGTLLAILVTTIVYLLTVWMTGSTVVAFSNGTEPAVFNNSYFVPPDCTPDCPFGLVSYYQVVEMSSFWGPLITAGIFAATLSSALASLVSAPKVFQAVCKDRLFPRIDYFAKTYGKNEEPKRAYVLGFFLAMGIVAIGDLNVIAPIISNFFLGSYALINYACFDQSFADSPGFRPGFTYYNMWISLVGALLCVFVMFIIDWFSALVTFFCFGAIFMYLLHRKPDVNWGSSTQAHSYKNALSAMIKLSTTEEHVKNYRPQVLVLSGNPASRSCLVDFANNITKGSSLLVCGQVVPYEPSDRVYMVMKRLDENINVWLRKRHLKAFYRAVANSNFRKGAQSLIQLTGIAKMKPNIVLMGFKSNWYKGGPTETNLNEMNEYFGTIQDVFDWNMAMCVLRNGQVGLDFSEAMRNLNLIEPNRLNVPPIEEKEKEKEKTNENSPETVHLIEKDETARTDKSGDDGSSSISINETYVSDENQEDADGDDDNDNDGEESGADDEHQKEDDVELGVIDEQEKRHFSLRRRGSRRHTVEQKALLSSIQRFNRKVKKGTIDVWWLYDDGGLTLLIPHLLAIPKSYLEGARLRIFTISTSSRTMEQEQRGMAALLSKFRIDYSDVYVIADIGKKPRQETMNTWQSVIDPFIAQDGSCPIGMTTKSELSAQRDKTYRQLRAGELLNEHSINADLIVMTLPVPRKGMVSSSLYMSWLEVMTRNLPPVLLVRGNQQSVLTFYS

Target ORF information:

RefSeq Version	NM_001083255.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Na-K-Cl Cotransporter homolog (nkcc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083255.3

ORF Insert Sequence:

ATGAATGGAA ATGGAGAAAA TGGGACGGAT GAAAAGCAGA AACTCTTGTC CGTTGTTGGT 
GTTGTGTCAG AAGAGGTTGT CGAGATGAAT CGCGTCTCGT CGGCCAGGTT TCAGGTCAGT 
AAAACTGACG AGGACGAGAA AACCAGCGAC GACAATTTAC GAAAATTAAA AAGTGGCAGT 
GAGCCGCCTC CTCCGACTCA AAATCATGAA GCAGTGACGC TTGTGACGAG AAAAATGAGT 
GCCACTGGAA GATTTATGGT AACCTCTGAC ACAAATCCGG GTCAACTCGT CGCTGAAGTC 
GCTGATGAAC TTGGTATTGA TGTTCCGATA GATCCGCCGG CGGAAAAATC GCCGGCTTCA 
CGTGCTAAAG GAGTACATTT CTCTGTTGGA GACAAGGATT CCGGTAGCAT GTCGGAGGAT 
GGCGACGAGA AACGAAATAT AACACACGAG AATCAGACAA CGTTCAATAT GAAAAGTTGG 
AGAAATATGA AGACGATAGA GCATCCGCCG ATTATCGATT TTTATCGGAA TTCGATTGAT 
ACAGATGGGG TGTTCAACAG GCCTTCGATG GCTCAACTTA TTCATGGAAA AGAGCACAAA 
GAGCAAGATC TCGGATTTGA GGAGTTGAAT GCTCAACACC ACCATCTCGA AGATTCTCCA 
GTTCACGAGA CGAAAAACGA GTATCGAATG GAGAAAATGC ACGCGCCTCC ATCGCAAAAT 
CGAGTGAAAT TCGGTTGGAT TCAGGGAGTA TTCGTCAGAT GTCTCCTGAA TATCTTCGGA 
GTTATGCTTT ATCTTCGTGT CAGTTGGGTT TCTGGTCAAG CTGGCGTTGG ACTGGGTTCC 
TGTATTGTTC TGCTGGCTTC TGTCGTCACA ACGATTACGG CACTTTCCAC GTGTGCAATT 
TGTACGAATG GAGATGTGAA GGGCGGTGGC GCGTATTTTT TGATTTCCCG TTCACTTGGT 
CCTGAATTCG GTGGCTCTAT TGGAATCATT TTCTCAATTG CAAATGCAGT CGGTGCAGCA 
ATGTATATCG TCGGTTTCGC CGAATCGTTT CGTGATGTTC TCATCGACTA TAATATTGGA 
ACGATATTTG ATGGAGGTCT TTGGGATGTT CGTGTTATTG GATTCGTGAC ATGCGTGATT 
CTCATGGGAA TTGTGTTCAT TGGCTCCGAG TTTGAATCGA AAATGCAGCT TGGACTCCTC 
GTGATTCTGC TCGTTTCCAT CGCCAACTAC GTCTTCGGAT CGTTCTTCCC GCCAAATGAG 
ATTGCAATGA ATCGAGGTGC AACTGGATAT TCACTGGATA CTCTACAATC CAACTTTATG 
CCACATTTCA CTGAAAACAA CACTTTCTTC TCAGTATTCT CCGTCTATTT CCCCGCTGCC 
ACTGGAATTA TGGCCGGCGC GAACATTAGT GGAGATCTAG CGAATCCTCA ACAAGCCATC 
CCACTTGGAA CACTTCTGGC AATTCTTGTC ACAACTATCG TCTATTTGTT AACAGTTTGG 
ATGACTGGAA GTACTGTAGT TGCGTTTTCA AATGGAACAG AGCCGGCTGT CTTCAATAAT 
TCGTATTTTG TTCCACCAGA TTGCACGCCG GATTGTCCGT TTGGATTGGT TTCTTATTAT 
CAGGTCGTCG AAATGTCATC CTTCTGGGGT CCTCTCATCA CTGCTGGAAT CTTTGCCGCA 
ACTCTATCCT CTGCTCTCGC CAGTCTTGTC AGTGCTCCGA AGGTGTTCCA GGCGGTCTGC 
AAAGATCGGC TTTTCCCGAG AATCGACTAT TTTGCGAAGA CTTATGGAAA GAATGAGGAA 
CCAAAGAGAG CATATGTTCT CGGATTTTTC TTGGCGATGG GGATCGTTGC AATTGGTGAC 
CTGAACGTGA TTGCTCCAAT CATTTCGAAC TTCTTCCTCG GCTCCTACGC TCTGATAAAC 
TACGCCTGCT TCGATCAGTC CTTTGCCGAT TCACCGGGCT TCCGACCGGG CTTCACCTAC 
TACAACATGT GGATCTCCCT CGTCGGAGCA CTTCTCTGTG TCTTTGTCAT GTTCATCATC 
GATTGGTTCT CGGCTCTCGT CACTTTCTTC TGTTTTGGAG CCATTTTCAT GTATTTATTG 
CATAGAAAAC CCGATGTTAA TTGGGGTTCA TCGACACAGG CACACAGTTA CAAGAACGCG 
TTGTCCGCTA TGATTAAGCT TTCGACTACC GAAGAGCACG TGAAGAATTA CAGGCCGCAG 
GTGTTGGTAC TTAGTGGAAA TCCGGCGAGT CGATCGTGTT TGGTGGATTT CGCCAATAAT 
ATTACGAAAG GAAGTAGTTT GTTGGTTTGT GGACAAGTCG TACCGTATGA GCCATCTGAT 
CGTGTCTATA TGGTGATGAA GCGTCTCGAC GAGAATATCA ACGTCTGGCT TCGAAAACGT 
CATCTCAAAG CGTTCTACCG TGCTGTCGCC AACTCGAATT TCCGGAAAGG TGCTCAATCG 
TTGATCCAAC TCACTGGAAT CGCGAAGATG AAGCCGAATA TAGTTTTGAT GGGATTCAAA 
TCGAACTGGT ATAAAGGAGG ACCGACGGAG ACGAATTTGA ACGAGATGAA CGAATATTTT 
GGAACTATTC AAGACGTTTT CGACTGGAAT ATGGCAATGT GTGTTCTACG AAATGGCCAA 
GTCGGTCTCG ACTTTTCTGA AGCAATGAGA AATTTGAATC TTATTGAACC GAATCGATTA 
AATGTTCCAC CGATTGAGGA GAAGGAGAAA GAAAAAGAGA AAACGAATGA AAACTCGCCG 
GAAACGGTTC ATTTAATCGA AAAAGACGAG ACAGCGAGAA CTGACAAGTC AGGAGACGAT 
GGTTCTTCAT CCATCTCAAT CAACGAAACA TACGTATCAG ATGAGAATCA AGAAGACGCA 
GATGGCGATG ATGATAATGA TAATGATGGA GAAGAATCTG GAGCTGATGA TGAGCATCAG 
AAGGAAGATG ATGTCGAGTT GGGCGTTATT GATGAGCAGG AAAAGCGACA TTTCTCGTTG 
AGGCGGAGAG GATCTCGACG TCATACTGTT GAGCAGAAGG CGCTTCTCTC GTCAATTCAG 
CGCTTCAATC GGAAGGTCAA GAAGGGAACC ATCGACGTTT GGTGGTTGTA TGATGACGGA 
GGTCTCACTC TTCTCATCCC ACACCTTCTC GCCATACCGA AAAGCTATCT GGAAGGTGCC 
CGCCTTCGGA TCTTCACCAT CTCCACGTCA TCCCGAACGA TGGAACAAGA GCAACGTGGA 
ATGGCGGCTC TTTTGTCGAA ATTCCGAATC GATTATTCGG ATGTCTACGT CATCGCTGAT 
ATTGGCAAGA AGCCGCGTCA GGAGACGATG AACACGTGGC AATCGGTAAT CGATCCGTTC 
ATCGCGCAGG ACGGAAGCTG TCCGATTGGA ATGACGACGA AGTCGGAGTT GAGTGCTCAA 
CGAGACAAGA CGTATCGGCA GTTGAGAGCT GGAGAGCTTC TGAATGAGCA TTCGATAAAT 
GCAGATCTTA TTGTGATGAC TCTACCAGTA CCACGCAAAG GAATGGTTAG CTCATCATTG 
TACATGTCGT GGCTGGAAGT GATGACTCGA AACTTGCCTC CAGTACTCCT CGTTCGCGGA 
AATCAACAAT CCGTCTTGAC TTTCTATTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nkcc-1
Entrez Gene ID	3565648
Full Name	Na-K-Cl Cotransporter homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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nkcc-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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