Slc12a1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
NM_183354.2	NP_899197.2	solute carrier family 12 member 1 isoform A
XM_030248889.1	XP_030104749.1	solute carrier family 12 member 1 isoform X4
XM_030248891.1	XP_030104751.1	solute carrier family 12 member 1 isoform X5
XM_030248892.1	XP_030104752.1	solute carrier family 12 member 1 isoform X6
XM_030248890.1	XP_030104750.1	solute carrier family 12 member 1 isoform X5
NM_001079690.2	NP_001073158.2	solute carrier family 12 member 1 isoform F
NM_183354.3	NP_899197.3	solute carrier family 12 member 1 isoform A
NM_001079690.1	NP_001073158.1	solute carrier family 12 member 1 isoform F
XM_030248885.1	XP_030104745.1	solute carrier family 12 member 1 isoform X1
XM_030248886.1	XP_030104746.1	solute carrier family 12 member 1 isoform X1
XM_030248887.1	XP_030104747.1	solute carrier family 12 member 1 isoform X2
XM_030248888.1	XP_030104748.1	solute carrier family 12 member 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20760	NM_183354.2 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu84631	NM_001079690.2 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84632	NM_183354.3 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84631	XM_030248889.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84632	XM_030248892.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87766	XM_030248886.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87766	XM_030248885.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87767	XM_030248887.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87768	XM_030248888.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87769	XM_030248890.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu87769	XM_030248891.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu20761	NM_001079690.1 Latest version!	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

Slc12a1 ( NM_183354.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_899197.2 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20760
Clone ID Related Accession (Same CDS sequence)	NM_183354.2
Accession Version	NM_183354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052750.1, U20973.1, AL929451.8, AI527944.1, AI875508.1 and AL844547.9. On Dec 9, 2006 this sequence version replaced NM_183354.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAATAC TACCGCAACA CAGGCAGCGT GAGCGGGCCC 
AAGGTCAACC GGCCCAGCCT GCTGGAGATC CACGAGCAGC TGGCTAAGAA TGTGACTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACAT GACTGGAGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGCGATGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT TGGAGTTCTT ATAATTCTTC TTTCCACCAT GGTAACCTCT 
ATCACTGGGT TGTCAACTTC TGCAATAGCA ACAAACGGGT TTGTTCGTGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATCTTT 
GCTTTTGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GATTCGCGGA GACTGTCGTG 
GATCTTCTTA AGGAGAGCGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGCATCATC 
GGTTCCATCA CCGTGGTGAT TCTTCTAGGA ATCTCAGTAG CTGGGATGGA GTGGGAAGCA 
AAGGCTCAAG TCATTCTCCT GGTCATTCTT CTCATTGCCA TTGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA TGAGAAGAAA TCCAGAGGCT TCTTTAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAAAGGGGG AAGGCTTCTT CTCGGTCTTT 
GCCATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATCTTCAT CACCACCGTG 
GCCTACATAG GTGTTGCTAT TTGCGTAGCC GCCTGTGTGG TCCGAGATGC CACTGGCAGC 
ATGAATGACA CCATCGTTTC TGGGATGAAT TGCAATGGCT CTGCGGCCTG TGGGTTGGGC 
TACGACTTCT CAAGATGTCA ACACGAGCCA TGTCAGTATG GGCTGATGAA CAATTTCCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGGCCC CTCATCACTG CAGGGATCTT TTCGGCAACA 
CTGTCTTCCG CCCTGGCCTC CCTCGTCAGT GCGCCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT GCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AATGTCATTG CCCCCATCAT CTCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGTTTCC ATGCCTCGTA TGCCAAATCT CCAGGATGGA GGCCTGCATA TGGGATTTAC 
AACATGTGGG TATCTCTTTT TGGAGCGATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCAG CTGTCATCAC TTATGTCATT GAGTTATTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTATGT GAGTGCTTTA 
GACAACGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGCGGACC CATGACTAGA CCCGCTCTCT TGGATATAAC CCACGCCTTT 
ACGAAGAATA GTGGCCTCTG CATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGCGG CATGGCTAAA AAACAGGCCT GGCTTATAAA GAACAAAATC 
AAAGCTTTTT ATGCTGCAGT GGCTGCAGAC TGCTTCAGGG ACGGTGTCCG GAGCCTCCTT 
CAGGCCTCAG GCTTGGGGAG AATGAAGCCA AACACTCTCG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC AGAGCTGGAG AACTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGCGTGGT CATAGTCAGA ATCAGCCAAG GCTTTGACAT CTCTCCGGTT 
CTCCAGGTAC AAGACGAACT GGAGAAACTG GAACAGGAGA GATTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGAGTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAGCTGA ACATTACCAA GCCAGCCCCC AAGAAAGACG GCAACATCAG CAGCATCCAG 
TCAATGCACG TCGGGGAGTT CAATCAGAAA CTGGTGGAAG CCAGCGCTCA GTTTAAAAAG 
AAGCAGGGAA AAGGCACGAT TGATGTTTGG TGGTTATTTG ATGATGGAGG GTTAACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAA AAATGGAAAG ATTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AGATCAACCG CATCGAAGAG GAAAAAATTT CAATGGCTTC ACTTCTCAGC 
AAATTTAGAA TAAAATTTGC AGACATCCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATCGGCTTCA TGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGTCAAGTTC GACTGAACGA GCTCTTACAG 
GAACACTCCA GAGCCGCCAA CCTCATCGTG TTGAGCCTTC CAGTGGCAAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT TCCTCCTGTG 
TTACTGGTTA GAGGAAATCA CAAAAATGTC TTAACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899197.2
CDS	233..3520
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_183354.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183354.2

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAATAC TACCGCAACA CAGGCAGCGT GAGCGGGCCC 
AAGGTCAACC GGCCCAGCCT GCTGGAGATC CACGAGCAGC TGGCTAAGAA TGTGACTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACAT GACTGGAGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGCGATGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT TGGAGTTCTT ATAATTCTTC TTTCCACCAT GGTAACCTCT 
ATCACTGGGT TGTCAACTTC TGCAATAGCA ACAAACGGGT TTGTTCGTGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATCTTT 
GCTTTTGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GATTCGCGGA GACTGTCGTG 
GATCTTCTTA AGGAGAGCGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGCATCATC 
GGTTCCATCA CCGTGGTGAT TCTTCTAGGA ATCTCAGTAG CTGGGATGGA GTGGGAAGCA 
AAGGCTCAAG TCATTCTCCT GGTCATTCTT CTCATTGCCA TTGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA TGAGAAGAAA TCCAGAGGCT TCTTTAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAAAGGGGG AAGGCTTCTT CTCGGTCTTT 
GCCATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATCTTCAT CACCACCGTG 
GCCTACATAG GTGTTGCTAT TTGCGTAGCC GCCTGTGTGG TCCGAGATGC CACTGGCAGC 
ATGAATGACA CCATCGTTTC TGGGATGAAT TGCAATGGCT CTGCGGCCTG TGGGTTGGGC 
TACGACTTCT CAAGATGTCA ACACGAGCCA TGTCAGTATG GGCTGATGAA CAATTTCCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGGCCC CTCATCACTG CAGGGATCTT TTCGGCAACA 
CTGTCTTCCG CCCTGGCCTC CCTCGTCAGT GCGCCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT GCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AATGTCATTG CCCCCATCAT CTCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGTTTCC ATGCCTCGTA TGCCAAATCT CCAGGATGGA GGCCTGCATA TGGGATTTAC 
AACATGTGGG TATCTCTTTT TGGAGCGATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCAG CTGTCATCAC TTATGTCATT GAGTTATTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTATGT GAGTGCTTTA 
GACAACGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGCGGACC CATGACTAGA CCCGCTCTCT TGGATATAAC CCACGCCTTT 
ACGAAGAATA GTGGCCTCTG CATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGCGG CATGGCTAAA AAACAGGCCT GGCTTATAAA GAACAAAATC 
AAAGCTTTTT ATGCTGCAGT GGCTGCAGAC TGCTTCAGGG ACGGTGTCCG GAGCCTCCTT 
CAGGCCTCAG GCTTGGGGAG AATGAAGCCA AACACTCTCG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC AGAGCTGGAG AACTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGCGTGGT CATAGTCAGA ATCAGCCAAG GCTTTGACAT CTCTCCGGTT 
CTCCAGGTAC AAGACGAACT GGAGAAACTG GAACAGGAGA GATTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGAGTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAGCTGA ACATTACCAA GCCAGCCCCC AAGAAAGACG GCAACATCAG CAGCATCCAG 
TCAATGCACG TCGGGGAGTT CAATCAGAAA CTGGTGGAAG CCAGCGCTCA GTTTAAAAAG 
AAGCAGGGAA AAGGCACGAT TGATGTTTGG TGGTTATTTG ATGATGGAGG GTTAACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAA AAATGGAAAG ATTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AGATCAACCG CATCGAAGAG GAAAAAATTT CAATGGCTTC ACTTCTCAGC 
AAATTTAGAA TAAAATTTGC AGACATCCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATCGGCTTCA TGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGTCAAGTTC GACTGAACGA GCTCTTACAG 
GAACACTCCA GAGCCGCCAA CCTCATCGTG TTGAGCCTTC CAGTGGCAAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT TCCTCCTGTG 
TTACTGGTTA GAGGAAATCA CAAAAATGTC TTAACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( NM_001079690.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001073158.2 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84631
Clone ID Related Accession (Same CDS sequence)	XM_030248889.1 , NM_001079690.2
Accession Version	NM_001079690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052750.1, AL929451.8, BY716143.1 and AL844547.9. On Feb 2, 2019 this sequence version replaced NM_001079690.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U94518.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTGGGCG TCATCATCAT TGGCCTGAGC GTAGTTGTGA CAACACTCAC AGGTATTTCT 
ATGTCGGCTA TTTGCACAAA CGGAGTGGTG CGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073158.2
CDS	233..3505
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_001079690.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079690.2

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTGGGCG TCATCATCAT TGGCCTGAGC GTAGTTGTGA CAACACTCAC AGGTATTTCT 
ATGTCGGCTA TTTGCACAAA CGGAGTGGTG CGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( NM_183354.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_899197.3 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 2, mRNA.

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CloneID	OMu84632
Clone ID Related Accession (Same CDS sequence)	XM_030248892.1 , NM_183354.3
Accession Version	NM_183354.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052750.1, AL929451.8, BY716143.1 and AL844547.9. On Feb 2, 2019 this sequence version replaced NM_183354.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTTGGAG TTCTTATAAT TCTTCTTTCC ACCATGGTAA CCTCTATCAC TGGGTTGTCA 
ACTTCTGCAA TAGCAACAAA CGGGTTTGTT CGTGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899197.3
CDS	233..3505
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_183354.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183354.3

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTTGGAG TTCTTATAAT TCTTCTTTCC ACCATGGTAA CCTCTATCAC TGGGTTGTCA 
ACTTCTGCAA TAGCAACAAA CGGGTTTGTT CGTGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248889.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104749.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu84631
Clone ID Related Accession (Same CDS sequence)	XM_030248889.1 , NM_001079690.2
Accession Version	XM_030248889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTGGGCG TCATCATCAT TGGCCTGAGC GTAGTTGTGA CAACACTCAC AGGTATTTCT 
ATGTCGGCTA TTTGCACAAA CGGAGTGGTG CGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104749.1
CDS	271..3543
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248889.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248889.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTGGGCG TCATCATCAT TGGCCTGAGC GTAGTTGTGA CAACACTCAC AGGTATTTCT 
ATGTCGGCTA TTTGCACAAA CGGAGTGGTG CGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248892.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104752.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X8, mRNA.

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CloneID	OMu84632
Clone ID Related Accession (Same CDS sequence)	XM_030248892.1 , NM_183354.3
Accession Version	XM_030248892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTTGGAG TTCTTATAAT TCTTCTTTCC ACCATGGTAA CCTCTATCAC TGGGTTGTCA 
ACTTCTGCAA TAGCAACAAA CGGGTTTGTT CGTGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104752.1
CDS	271..3543
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248892.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248892.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTTGGAG TTCTTATAAT TCTTCTTTCC ACCATGGTAA CCTCTATCAC TGGGTTGTCA 
ACTTCTGCAA TAGCAACAAA CGGGTTTGTT CGTGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248885.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104745.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87766
Clone ID Related Accession (Same CDS sequence)	XM_030248885.1 , XM_030248886.1
Accession Version	XM_030248885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTC TTGGAGTTCT TATAATTCTT 
CTTTCCACCA TGGTAACCTC TATCACTGGG TTGTCAACTT CTGCAATAGC AACAAACGGG 
TTTGTTCGTG GAGGTCTGGG CGTCATCATC ATTGGCCTGA GCGTAGTTGT GACAACACTC 
ACAGGTATTT CTATGTCGGC TATTTGCACA AACGGAGTGG TGCGAGGAGG TGGAGCCTAC 
TACCTGATTT CCCGGAGCTT AGGGCCTGAG TTCGGTGGGT CAATAGGCTT GATCTTTGCT 
TTTGCCAATG CAGTGGCTGT CGCTATGTAT GTGGTGGGAT TCGCGGAGAC TGTCGTGGAT 
CTTCTTAAGG AGAGCGATTC AATGATGGTG GATCCAACCA ATGACATCCG CATCATCGGT 
TCCATCACCG TGGTGATTCT TCTAGGAATC TCAGTAGCTG GGATGGAGTG GGAAGCAAAG 
GCTCAAGTCA TTCTCCTGGT CATTCTTCTC ATTGCCATTG CAAACTTCTT CATTGGAACT 
GTCATCCCAT CCAACAATGA GAAGAAATCC AGAGGCTTCT TTAACTACCA AGCATCTATA 
TTTGCGGAAA ACTTTGGGCC AAGCTTCACA AAGGGGGAAG GCTTCTTCTC GGTCTTTGCC 
ATCTTTTTCC CAGCTGCTAC TGGGATTCTT GCTGGTGCCA ACATCTCTGG AGACCTGGAG 
GATCCCCAGG ACGCCATCCC CAGAGGGACC ATGCTGGCCA TCTTCATCAC CACCGTGGCC 
TACATAGGTG TTGCTATTTG CGTAGCCGCC TGTGTGGTCC GAGATGCCAC TGGCAGCATG 
AATGACACCA TCGTTTCTGG GATGAATTGC AATGGCTCTG CGGCCTGTGG GTTGGGCTAC 
GACTTCTCAA GATGTCAACA CGAGCCATGT CAGTATGGGC TGATGAACAA TTTCCAGGTC 
ATGAGCATGG TGTCCGGGTT CGGGCCCCTC ATCACTGCAG GGATCTTTTC GGCAACACTG 
TCTTCCGCCC TGGCCTCCCT CGTCAGTGCG CCCAAAGTAT TCCAGGCTCT GTGCAAGGAC 
AACATCTTCA AAGGACTGCA GTTCTTCGCA AAGGGCTATG GGAAAAACAA CGAGCCCCTG 
AGAGGGTACT TCCTCACTTT TGTGATAGCC ATGGCATTCA TTCTCATCGC CGAGCTGAAT 
GTCATTGCCC CCATCATCTC CAACTTCTTC CTGGCCTCAT ATGCGCTTAT TAATTTCTCC 
TGTTTCCATG CCTCGTATGC CAAATCTCCA GGATGGAGGC CTGCATATGG GATTTACAAC 
ATGTGGGTAT CTCTTTTTGG AGCGATTTTG TGCTGTGCAG TCATGTTTGT CATCAACTGG 
TGGGCAGCTG TCATCACTTA TGTCATTGAG TTATTCCTCT ATATCTATGT GACTTATAAG 
AAGCCAGATG TGAACTGGGG CTCCTCCACA CAGGCTCTGT CCTATGTGAG TGCTTTAGAC 
AACGCTCTGG AATTAACCAC AGTGGAAGAC CATGTGAAAA ACTTCAGACC CCAGTGCATT 
GTCTTAACAG GCGGACCCAT GACTAGACCC GCTCTCTTGG ATATAACCCA CGCCTTTACG 
AAGAATAGTG GCCTCTGCAT CTGCTGCGAA GTCTTCGTGG GACCTCGCAA GCTGTGTGTT 
AAAGAGATGA ACAGCGGCAT GGCTAAAAAA CAGGCCTGGC TTATAAAGAA CAAAATCAAA 
GCTTTTTATG CTGCAGTGGC TGCAGACTGC TTCAGGGACG GTGTCCGGAG CCTCCTTCAG 
GCCTCAGGCT TGGGGAGAAT GAAGCCAAAC ACTCTCGTGA TTGGATACAA AAAGAACTGG 
AGGAAAGCTC CCTTGTCAGA GCTGGAGAAC TACGTGGGAA TCATACATGA CGCCTTTGAC 
TTTGAGATTG GCGTGGTCAT AGTCAGAATC AGCCAAGGCT TTGACATCTC TCCGGTTCTC 
CAGGTACAAG ACGAACTGGA GAAACTGGAA CAGGAGAGAT TGGCTCTGGA GGCCGCAATC 
AAAGACAACG AGTGTGAGGA GGGCAAGGGG GGCATCCGAG GCTTGTTTAA GAAGGCCGGC 
AAGCTGAACA TTACCAAGCC AGCCCCCAAG AAAGACGGCA ACATCAGCAG CATCCAGTCA 
ATGCACGTCG GGGAGTTCAA TCAGAAACTG GTGGAAGCCA GCGCTCAGTT TAAAAAGAAG 
CAGGGAAAAG GCACGATTGA TGTTTGGTGG TTATTTGATG ATGGAGGGTT AACACTGCTT 
ATCCCATATA TCTTGACTCT CAGAAAAAAA TGGAAAGATT GTAAGTTAAG AATCTATGTT 
GGAGGAAAGA TCAACCGCAT CGAAGAGGAA AAAATTTCAA TGGCTTCACT TCTCAGCAAA 
TTTAGAATAA AATTTGCAGA CATCCATATA ATTGGAGACA TCAACATTAA GCCAAACAAA 
GAGAGCTGGA AAGTCTTTGA AGAGATGATT GAACCTTATC GGCTTCATGA AAGCCACAAA 
GATTTGACCA CTGCTGAGAA ATTAAAAAGA GAAAGTCCAT GGAAAATTAC AGATGCAGAA 
CTGGAAGCAG TCAAGGAAAA GAGTTACCGT CAAGTTCGAC TGAACGAGCT CTTACAGGAA 
CACTCCAGAG CCGCCAACCT CATCGTGTTG AGCCTTCCAG TGGCAAGAAA AGGATCTATT 
TCGGATTTGT TGTACATGGC GTGGTTAGAA ATCCTGACCA AGAACCTTCC TCCTGTGTTA 
CTGGTTAGAG GAAATCACAA AAATGTCTTA ACATTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104745.1
CDS	323..3787
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248885.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248885.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTC TTGGAGTTCT TATAATTCTT 
CTTTCCACCA TGGTAACCTC TATCACTGGG TTGTCAACTT CTGCAATAGC AACAAACGGG 
TTTGTTCGTG GAGGTCTGGG CGTCATCATC ATTGGCCTGA GCGTAGTTGT GACAACACTC 
ACAGGTATTT CTATGTCGGC TATTTGCACA AACGGAGTGG TGCGAGGAGG TGGAGCCTAC 
TACCTGATTT CCCGGAGCTT AGGGCCTGAG TTCGGTGGGT CAATAGGCTT GATCTTTGCT 
TTTGCCAATG CAGTGGCTGT CGCTATGTAT GTGGTGGGAT TCGCGGAGAC TGTCGTGGAT 
CTTCTTAAGG AGAGCGATTC AATGATGGTG GATCCAACCA ATGACATCCG CATCATCGGT 
TCCATCACCG TGGTGATTCT TCTAGGAATC TCAGTAGCTG GGATGGAGTG GGAAGCAAAG 
GCTCAAGTCA TTCTCCTGGT CATTCTTCTC ATTGCCATTG CAAACTTCTT CATTGGAACT 
GTCATCCCAT CCAACAATGA GAAGAAATCC AGAGGCTTCT TTAACTACCA AGCATCTATA 
TTTGCGGAAA ACTTTGGGCC AAGCTTCACA AAGGGGGAAG GCTTCTTCTC GGTCTTTGCC 
ATCTTTTTCC CAGCTGCTAC TGGGATTCTT GCTGGTGCCA ACATCTCTGG AGACCTGGAG 
GATCCCCAGG ACGCCATCCC CAGAGGGACC ATGCTGGCCA TCTTCATCAC CACCGTGGCC 
TACATAGGTG TTGCTATTTG CGTAGCCGCC TGTGTGGTCC GAGATGCCAC TGGCAGCATG 
AATGACACCA TCGTTTCTGG GATGAATTGC AATGGCTCTG CGGCCTGTGG GTTGGGCTAC 
GACTTCTCAA GATGTCAACA CGAGCCATGT CAGTATGGGC TGATGAACAA TTTCCAGGTC 
ATGAGCATGG TGTCCGGGTT CGGGCCCCTC ATCACTGCAG GGATCTTTTC GGCAACACTG 
TCTTCCGCCC TGGCCTCCCT CGTCAGTGCG CCCAAAGTAT TCCAGGCTCT GTGCAAGGAC 
AACATCTTCA AAGGACTGCA GTTCTTCGCA AAGGGCTATG GGAAAAACAA CGAGCCCCTG 
AGAGGGTACT TCCTCACTTT TGTGATAGCC ATGGCATTCA TTCTCATCGC CGAGCTGAAT 
GTCATTGCCC CCATCATCTC CAACTTCTTC CTGGCCTCAT ATGCGCTTAT TAATTTCTCC 
TGTTTCCATG CCTCGTATGC CAAATCTCCA GGATGGAGGC CTGCATATGG GATTTACAAC 
ATGTGGGTAT CTCTTTTTGG AGCGATTTTG TGCTGTGCAG TCATGTTTGT CATCAACTGG 
TGGGCAGCTG TCATCACTTA TGTCATTGAG TTATTCCTCT ATATCTATGT GACTTATAAG 
AAGCCAGATG TGAACTGGGG CTCCTCCACA CAGGCTCTGT CCTATGTGAG TGCTTTAGAC 
AACGCTCTGG AATTAACCAC AGTGGAAGAC CATGTGAAAA ACTTCAGACC CCAGTGCATT 
GTCTTAACAG GCGGACCCAT GACTAGACCC GCTCTCTTGG ATATAACCCA CGCCTTTACG 
AAGAATAGTG GCCTCTGCAT CTGCTGCGAA GTCTTCGTGG GACCTCGCAA GCTGTGTGTT 
AAAGAGATGA ACAGCGGCAT GGCTAAAAAA CAGGCCTGGC TTATAAAGAA CAAAATCAAA 
GCTTTTTATG CTGCAGTGGC TGCAGACTGC TTCAGGGACG GTGTCCGGAG CCTCCTTCAG 
GCCTCAGGCT TGGGGAGAAT GAAGCCAAAC ACTCTCGTGA TTGGATACAA AAAGAACTGG 
AGGAAAGCTC CCTTGTCAGA GCTGGAGAAC TACGTGGGAA TCATACATGA CGCCTTTGAC 
TTTGAGATTG GCGTGGTCAT AGTCAGAATC AGCCAAGGCT TTGACATCTC TCCGGTTCTC 
CAGGTACAAG ACGAACTGGA GAAACTGGAA CAGGAGAGAT TGGCTCTGGA GGCCGCAATC 
AAAGACAACG AGTGTGAGGA GGGCAAGGGG GGCATCCGAG GCTTGTTTAA GAAGGCCGGC 
AAGCTGAACA TTACCAAGCC AGCCCCCAAG AAAGACGGCA ACATCAGCAG CATCCAGTCA 
ATGCACGTCG GGGAGTTCAA TCAGAAACTG GTGGAAGCCA GCGCTCAGTT TAAAAAGAAG 
CAGGGAAAAG GCACGATTGA TGTTTGGTGG TTATTTGATG ATGGAGGGTT AACACTGCTT 
ATCCCATATA TCTTGACTCT CAGAAAAAAA TGGAAAGATT GTAAGTTAAG AATCTATGTT 
GGAGGAAAGA TCAACCGCAT CGAAGAGGAA AAAATTTCAA TGGCTTCACT TCTCAGCAAA 
TTTAGAATAA AATTTGCAGA CATCCATATA ATTGGAGACA TCAACATTAA GCCAAACAAA 
GAGAGCTGGA AAGTCTTTGA AGAGATGATT GAACCTTATC GGCTTCATGA AAGCCACAAA 
GATTTGACCA CTGCTGAGAA ATTAAAAAGA GAAAGTCCAT GGAAAATTAC AGATGCAGAA 
CTGGAAGCAG TCAAGGAAAA GAGTTACCGT CAAGTTCGAC TGAACGAGCT CTTACAGGAA 
CACTCCAGAG CCGCCAACCT CATCGTGTTG AGCCTTCCAG TGGCAAGAAA AGGATCTATT 
TCGGATTTGT TGTACATGGC GTGGTTAGAA ATCCTGACCA AGAACCTTCC TCCTGTGTTA 
CTGGTTAGAG GAAATCACAA AAATGTCTTA ACATTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248886.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104746.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu87766
Clone ID Related Accession (Same CDS sequence)	XM_030248885.1 , XM_030248886.1
Accession Version	XM_030248886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTC TTGGAGTTCT TATAATTCTT 
CTTTCCACCA TGGTAACCTC TATCACTGGG TTGTCAACTT CTGCAATAGC AACAAACGGG 
TTTGTTCGTG GAGGTCTGGG CGTCATCATC ATTGGCCTGA GCGTAGTTGT GACAACACTC 
ACAGGTATTT CTATGTCGGC TATTTGCACA AACGGAGTGG TGCGAGGAGG TGGAGCCTAC 
TACCTGATTT CCCGGAGCTT AGGGCCTGAG TTCGGTGGGT CAATAGGCTT GATCTTTGCT 
TTTGCCAATG CAGTGGCTGT CGCTATGTAT GTGGTGGGAT TCGCGGAGAC TGTCGTGGAT 
CTTCTTAAGG AGAGCGATTC AATGATGGTG GATCCAACCA ATGACATCCG CATCATCGGT 
TCCATCACCG TGGTGATTCT TCTAGGAATC TCAGTAGCTG GGATGGAGTG GGAAGCAAAG 
GCTCAAGTCA TTCTCCTGGT CATTCTTCTC ATTGCCATTG CAAACTTCTT CATTGGAACT 
GTCATCCCAT CCAACAATGA GAAGAAATCC AGAGGCTTCT TTAACTACCA AGCATCTATA 
TTTGCGGAAA ACTTTGGGCC AAGCTTCACA AAGGGGGAAG GCTTCTTCTC GGTCTTTGCC 
ATCTTTTTCC CAGCTGCTAC TGGGATTCTT GCTGGTGCCA ACATCTCTGG AGACCTGGAG 
GATCCCCAGG ACGCCATCCC CAGAGGGACC ATGCTGGCCA TCTTCATCAC CACCGTGGCC 
TACATAGGTG TTGCTATTTG CGTAGCCGCC TGTGTGGTCC GAGATGCCAC TGGCAGCATG 
AATGACACCA TCGTTTCTGG GATGAATTGC AATGGCTCTG CGGCCTGTGG GTTGGGCTAC 
GACTTCTCAA GATGTCAACA CGAGCCATGT CAGTATGGGC TGATGAACAA TTTCCAGGTC 
ATGAGCATGG TGTCCGGGTT CGGGCCCCTC ATCACTGCAG GGATCTTTTC GGCAACACTG 
TCTTCCGCCC TGGCCTCCCT CGTCAGTGCG CCCAAAGTAT TCCAGGCTCT GTGCAAGGAC 
AACATCTTCA AAGGACTGCA GTTCTTCGCA AAGGGCTATG GGAAAAACAA CGAGCCCCTG 
AGAGGGTACT TCCTCACTTT TGTGATAGCC ATGGCATTCA TTCTCATCGC CGAGCTGAAT 
GTCATTGCCC CCATCATCTC CAACTTCTTC CTGGCCTCAT ATGCGCTTAT TAATTTCTCC 
TGTTTCCATG CCTCGTATGC CAAATCTCCA GGATGGAGGC CTGCATATGG GATTTACAAC 
ATGTGGGTAT CTCTTTTTGG AGCGATTTTG TGCTGTGCAG TCATGTTTGT CATCAACTGG 
TGGGCAGCTG TCATCACTTA TGTCATTGAG TTATTCCTCT ATATCTATGT GACTTATAAG 
AAGCCAGATG TGAACTGGGG CTCCTCCACA CAGGCTCTGT CCTATGTGAG TGCTTTAGAC 
AACGCTCTGG AATTAACCAC AGTGGAAGAC CATGTGAAAA ACTTCAGACC CCAGTGCATT 
GTCTTAACAG GCGGACCCAT GACTAGACCC GCTCTCTTGG ATATAACCCA CGCCTTTACG 
AAGAATAGTG GCCTCTGCAT CTGCTGCGAA GTCTTCGTGG GACCTCGCAA GCTGTGTGTT 
AAAGAGATGA ACAGCGGCAT GGCTAAAAAA CAGGCCTGGC TTATAAAGAA CAAAATCAAA 
GCTTTTTATG CTGCAGTGGC TGCAGACTGC TTCAGGGACG GTGTCCGGAG CCTCCTTCAG 
GCCTCAGGCT TGGGGAGAAT GAAGCCAAAC ACTCTCGTGA TTGGATACAA AAAGAACTGG 
AGGAAAGCTC CCTTGTCAGA GCTGGAGAAC TACGTGGGAA TCATACATGA CGCCTTTGAC 
TTTGAGATTG GCGTGGTCAT AGTCAGAATC AGCCAAGGCT TTGACATCTC TCCGGTTCTC 
CAGGTACAAG ACGAACTGGA GAAACTGGAA CAGGAGAGAT TGGCTCTGGA GGCCGCAATC 
AAAGACAACG AGTGTGAGGA GGGCAAGGGG GGCATCCGAG GCTTGTTTAA GAAGGCCGGC 
AAGCTGAACA TTACCAAGCC AGCCCCCAAG AAAGACGGCA ACATCAGCAG CATCCAGTCA 
ATGCACGTCG GGGAGTTCAA TCAGAAACTG GTGGAAGCCA GCGCTCAGTT TAAAAAGAAG 
CAGGGAAAAG GCACGATTGA TGTTTGGTGG TTATTTGATG ATGGAGGGTT AACACTGCTT 
ATCCCATATA TCTTGACTCT CAGAAAAAAA TGGAAAGATT GTAAGTTAAG AATCTATGTT 
GGAGGAAAGA TCAACCGCAT CGAAGAGGAA AAAATTTCAA TGGCTTCACT TCTCAGCAAA 
TTTAGAATAA AATTTGCAGA CATCCATATA ATTGGAGACA TCAACATTAA GCCAAACAAA 
GAGAGCTGGA AAGTCTTTGA AGAGATGATT GAACCTTATC GGCTTCATGA AAGCCACAAA 
GATTTGACCA CTGCTGAGAA ATTAAAAAGA GAAAGTCCAT GGAAAATTAC AGATGCAGAA 
CTGGAAGCAG TCAAGGAAAA GAGTTACCGT CAAGTTCGAC TGAACGAGCT CTTACAGGAA 
CACTCCAGAG CCGCCAACCT CATCGTGTTG AGCCTTCCAG TGGCAAGAAA AGGATCTATT 
TCGGATTTGT TGTACATGGC GTGGTTAGAA ATCCTGACCA AGAACCTTCC TCCTGTGTTA 
CTGGTTAGAG GAAATCACAA AAATGTCTTA ACATTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104746.1
CDS	272..3736
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248886.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248886.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTC TTGGAGTTCT TATAATTCTT 
CTTTCCACCA TGGTAACCTC TATCACTGGG TTGTCAACTT CTGCAATAGC AACAAACGGG 
TTTGTTCGTG GAGGTCTGGG CGTCATCATC ATTGGCCTGA GCGTAGTTGT GACAACACTC 
ACAGGTATTT CTATGTCGGC TATTTGCACA AACGGAGTGG TGCGAGGAGG TGGAGCCTAC 
TACCTGATTT CCCGGAGCTT AGGGCCTGAG TTCGGTGGGT CAATAGGCTT GATCTTTGCT 
TTTGCCAATG CAGTGGCTGT CGCTATGTAT GTGGTGGGAT TCGCGGAGAC TGTCGTGGAT 
CTTCTTAAGG AGAGCGATTC AATGATGGTG GATCCAACCA ATGACATCCG CATCATCGGT 
TCCATCACCG TGGTGATTCT TCTAGGAATC TCAGTAGCTG GGATGGAGTG GGAAGCAAAG 
GCTCAAGTCA TTCTCCTGGT CATTCTTCTC ATTGCCATTG CAAACTTCTT CATTGGAACT 
GTCATCCCAT CCAACAATGA GAAGAAATCC AGAGGCTTCT TTAACTACCA AGCATCTATA 
TTTGCGGAAA ACTTTGGGCC AAGCTTCACA AAGGGGGAAG GCTTCTTCTC GGTCTTTGCC 
ATCTTTTTCC CAGCTGCTAC TGGGATTCTT GCTGGTGCCA ACATCTCTGG AGACCTGGAG 
GATCCCCAGG ACGCCATCCC CAGAGGGACC ATGCTGGCCA TCTTCATCAC CACCGTGGCC 
TACATAGGTG TTGCTATTTG CGTAGCCGCC TGTGTGGTCC GAGATGCCAC TGGCAGCATG 
AATGACACCA TCGTTTCTGG GATGAATTGC AATGGCTCTG CGGCCTGTGG GTTGGGCTAC 
GACTTCTCAA GATGTCAACA CGAGCCATGT CAGTATGGGC TGATGAACAA TTTCCAGGTC 
ATGAGCATGG TGTCCGGGTT CGGGCCCCTC ATCACTGCAG GGATCTTTTC GGCAACACTG 
TCTTCCGCCC TGGCCTCCCT CGTCAGTGCG CCCAAAGTAT TCCAGGCTCT GTGCAAGGAC 
AACATCTTCA AAGGACTGCA GTTCTTCGCA AAGGGCTATG GGAAAAACAA CGAGCCCCTG 
AGAGGGTACT TCCTCACTTT TGTGATAGCC ATGGCATTCA TTCTCATCGC CGAGCTGAAT 
GTCATTGCCC CCATCATCTC CAACTTCTTC CTGGCCTCAT ATGCGCTTAT TAATTTCTCC 
TGTTTCCATG CCTCGTATGC CAAATCTCCA GGATGGAGGC CTGCATATGG GATTTACAAC 
ATGTGGGTAT CTCTTTTTGG AGCGATTTTG TGCTGTGCAG TCATGTTTGT CATCAACTGG 
TGGGCAGCTG TCATCACTTA TGTCATTGAG TTATTCCTCT ATATCTATGT GACTTATAAG 
AAGCCAGATG TGAACTGGGG CTCCTCCACA CAGGCTCTGT CCTATGTGAG TGCTTTAGAC 
AACGCTCTGG AATTAACCAC AGTGGAAGAC CATGTGAAAA ACTTCAGACC CCAGTGCATT 
GTCTTAACAG GCGGACCCAT GACTAGACCC GCTCTCTTGG ATATAACCCA CGCCTTTACG 
AAGAATAGTG GCCTCTGCAT CTGCTGCGAA GTCTTCGTGG GACCTCGCAA GCTGTGTGTT 
AAAGAGATGA ACAGCGGCAT GGCTAAAAAA CAGGCCTGGC TTATAAAGAA CAAAATCAAA 
GCTTTTTATG CTGCAGTGGC TGCAGACTGC TTCAGGGACG GTGTCCGGAG CCTCCTTCAG 
GCCTCAGGCT TGGGGAGAAT GAAGCCAAAC ACTCTCGTGA TTGGATACAA AAAGAACTGG 
AGGAAAGCTC CCTTGTCAGA GCTGGAGAAC TACGTGGGAA TCATACATGA CGCCTTTGAC 
TTTGAGATTG GCGTGGTCAT AGTCAGAATC AGCCAAGGCT TTGACATCTC TCCGGTTCTC 
CAGGTACAAG ACGAACTGGA GAAACTGGAA CAGGAGAGAT TGGCTCTGGA GGCCGCAATC 
AAAGACAACG AGTGTGAGGA GGGCAAGGGG GGCATCCGAG GCTTGTTTAA GAAGGCCGGC 
AAGCTGAACA TTACCAAGCC AGCCCCCAAG AAAGACGGCA ACATCAGCAG CATCCAGTCA 
ATGCACGTCG GGGAGTTCAA TCAGAAACTG GTGGAAGCCA GCGCTCAGTT TAAAAAGAAG 
CAGGGAAAAG GCACGATTGA TGTTTGGTGG TTATTTGATG ATGGAGGGTT AACACTGCTT 
ATCCCATATA TCTTGACTCT CAGAAAAAAA TGGAAAGATT GTAAGTTAAG AATCTATGTT 
GGAGGAAAGA TCAACCGCAT CGAAGAGGAA AAAATTTCAA TGGCTTCACT TCTCAGCAAA 
TTTAGAATAA AATTTGCAGA CATCCATATA ATTGGAGACA TCAACATTAA GCCAAACAAA 
GAGAGCTGGA AAGTCTTTGA AGAGATGATT GAACCTTATC GGCTTCATGA AAGCCACAAA 
GATTTGACCA CTGCTGAGAA ATTAAAAAGA GAAAGTCCAT GGAAAATTAC AGATGCAGAA 
CTGGAAGCAG TCAAGGAAAA GAGTTACCGT CAAGTTCGAC TGAACGAGCT CTTACAGGAA 
CACTCCAGAG CCGCCAACCT CATCGTGTTG AGCCTTCCAG TGGCAAGAAA AGGATCTATT 
TCGGATTTGT TGTACATGGC GTGGTTAGAA ATCCTGACCA AGAACCTTCC TCCTGTGTTA 
CTGGTTAGAG GAAATCACAA AAATGTCTTA ACATTTTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248887.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104747.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu87767
Clone ID Related Accession (Same CDS sequence)	XM_030248887.1
Accession Version	XM_030248887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTTGGAG TTCTTATAAT TCTTCTTTCC ACCATGGTAA CCTCTATCAC TGGGTTGTCA 
ACTTCTGCAA TAGCAACAAA CGGGTTTGTT CGTGGAGGTC TGGGCGTCAT CATCATTGGC 
CTGAGCGTAG TTGTGACAAC ACTCACAGGT ATTTCTATGT CGGCTATTTG CACAAACGGA 
GTGGTGCGAG GAGGTGGAGC CTACTACCTG ATTTCCCGGA GCTTAGGGCC TGAGTTCGGT 
GGGTCAATAG GCTTGATCTT TGCTTTTGCC AATGCAGTGG CTGTCGCTAT GTATGTGGTG 
GGATTCGCGG AGACTGTCGT GGATCTTCTT AAGGAGAGCG ATTCAATGAT GGTGGATCCA 
ACCAATGACA TCCGCATCAT CGGTTCCATC ACCGTGGTGA TTCTTCTAGG AATCTCAGTA 
GCTGGGATGG AGTGGGAAGC AAAGGCTCAA GTCATTCTCC TGGTCATTCT TCTCATTGCC 
ATTGCAAACT TCTTCATTGG AACTGTCATC CCATCCAACA ATGAGAAGAA ATCCAGAGGC 
TTCTTTAACT ACCAAGCATC TATATTTGCG GAAAACTTTG GGCCAAGCTT CACAAAGGGG 
GAAGGCTTCT TCTCGGTCTT TGCCATCTTT TTCCCAGCTG CTACTGGGAT TCTTGCTGGT 
GCCAACATCT CTGGAGACCT GGAGGATCCC CAGGACGCCA TCCCCAGAGG GACCATGCTG 
GCCATCTTCA TCACCACCGT GGCCTACATA GGTGTTGCTA TTTGCGTAGC CGCCTGTGTG 
GTCCGAGATG CCACTGGCAG CATGAATGAC ACCATCGTTT CTGGGATGAA TTGCAATGGC 
TCTGCGGCCT GTGGGTTGGG CTACGACTTC TCAAGATGTC AACACGAGCC ATGTCAGTAT 
GGGCTGATGA ACAATTTCCA GGTCATGAGC ATGGTGTCCG GGTTCGGGCC CCTCATCACT 
GCAGGGATCT TTTCGGCAAC ACTGTCTTCC GCCCTGGCCT CCCTCGTCAG TGCGCCCAAA 
GTATTCCAGG CTCTGTGCAA GGACAACATC TTCAAAGGAC TGCAGTTCTT CGCAAAGGGC 
TATGGGAAAA ACAACGAGCC CCTGAGAGGG TACTTCCTCA CTTTTGTGAT AGCCATGGCA 
TTCATTCTCA TCGCCGAGCT GAATGTCATT GCCCCCATCA TCTCCAACTT CTTCCTGGCC 
TCATATGCGC TTATTAATTT CTCCTGTTTC CATGCCTCGT ATGCCAAATC TCCAGGATGG 
AGGCCTGCAT ATGGGATTTA CAACATGTGG GTATCTCTTT TTGGAGCGAT TTTGTGCTGT 
GCAGTCATGT TTGTCATCAA CTGGTGGGCA GCTGTCATCA CTTATGTCAT TGAGTTATTC 
CTCTATATCT ATGTGACTTA TAAGAAGCCA GATGTGAACT GGGGCTCCTC CACACAGGCT 
CTGTCCTATG TGAGTGCTTT AGACAACGCT CTGGAATTAA CCACAGTGGA AGACCATGTG 
AAAAACTTCA GACCCCAGTG CATTGTCTTA ACAGGCGGAC CCATGACTAG ACCCGCTCTC 
TTGGATATAA CCCACGCCTT TACGAAGAAT AGTGGCCTCT GCATCTGCTG CGAAGTCTTC 
GTGGGACCTC GCAAGCTGTG TGTTAAAGAG ATGAACAGCG GCATGGCTAA AAAACAGGCC 
TGGCTTATAA AGAACAAAAT CAAAGCTTTT TATGCTGCAG TGGCTGCAGA CTGCTTCAGG 
GACGGTGTCC GGAGCCTCCT TCAGGCCTCA GGCTTGGGGA GAATGAAGCC AAACACTCTC 
GTGATTGGAT ACAAAAAGAA CTGGAGGAAA GCTCCCTTGT CAGAGCTGGA GAACTACGTG 
GGAATCATAC ATGACGCCTT TGACTTTGAG ATTGGCGTGG TCATAGTCAG AATCAGCCAA 
GGCTTTGACA TCTCTCCGGT TCTCCAGGTA CAAGACGAAC TGGAGAAACT GGAACAGGAG 
AGATTGGCTC TGGAGGCCGC AATCAAAGAC AACGAGTGTG AGGAGGGCAA GGGGGGCATC 
CGAGGCTTGT TTAAGAAGGC CGGCAAGCTG AACATTACCA AGCCAGCCCC CAAGAAAGAC 
GGCAACATCA GCAGCATCCA GTCAATGCAC GTCGGGGAGT TCAATCAGAA ACTGGTGGAA 
GCCAGCGCTC AGTTTAAAAA GAAGCAGGGA AAAGGCACGA TTGATGTTTG GTGGTTATTT 
GATGATGGAG GGTTAACACT GCTTATCCCA TATATCTTGA CTCTCAGAAA AAAATGGAAA 
GATTGTAAGT TAAGAATCTA TGTTGGAGGA AAGATCAACC GCATCGAAGA GGAAAAAATT 
TCAATGGCTT CACTTCTCAG CAAATTTAGA ATAAAATTTG CAGACATCCA TATAATTGGA 
GACATCAACA TTAAGCCAAA CAAAGAGAGC TGGAAAGTCT TTGAAGAGAT GATTGAACCT 
TATCGGCTTC ATGAAAGCCA CAAAGATTTG ACCACTGCTG AGAAATTAAA AAGAGAAAGT 
CCATGGAAAA TTACAGATGC AGAACTGGAA GCAGTCAAGG AAAAGAGTTA CCGTCAAGTT 
CGACTGAACG AGCTCTTACA GGAACACTCC AGAGCCGCCA ACCTCATCGT GTTGAGCCTT 
CCAGTGGCAA GAAAAGGATC TATTTCGGAT TTGTTGTACA TGGCGTGGTT AGAAATCCTG 
ACCAAGAACC TTCCTCCTGT GTTACTGGTT AGAGGAAATC ACAAAAATGT CTTAACATTT 
TACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104747.1
CDS	323..3691
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248887.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248887.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTTGGAG TTCTTATAAT TCTTCTTTCC ACCATGGTAA CCTCTATCAC TGGGTTGTCA 
ACTTCTGCAA TAGCAACAAA CGGGTTTGTT CGTGGAGGTC TGGGCGTCAT CATCATTGGC 
CTGAGCGTAG TTGTGACAAC ACTCACAGGT ATTTCTATGT CGGCTATTTG CACAAACGGA 
GTGGTGCGAG GAGGTGGAGC CTACTACCTG ATTTCCCGGA GCTTAGGGCC TGAGTTCGGT 
GGGTCAATAG GCTTGATCTT TGCTTTTGCC AATGCAGTGG CTGTCGCTAT GTATGTGGTG 
GGATTCGCGG AGACTGTCGT GGATCTTCTT AAGGAGAGCG ATTCAATGAT GGTGGATCCA 
ACCAATGACA TCCGCATCAT CGGTTCCATC ACCGTGGTGA TTCTTCTAGG AATCTCAGTA 
GCTGGGATGG AGTGGGAAGC AAAGGCTCAA GTCATTCTCC TGGTCATTCT TCTCATTGCC 
ATTGCAAACT TCTTCATTGG AACTGTCATC CCATCCAACA ATGAGAAGAA ATCCAGAGGC 
TTCTTTAACT ACCAAGCATC TATATTTGCG GAAAACTTTG GGCCAAGCTT CACAAAGGGG 
GAAGGCTTCT TCTCGGTCTT TGCCATCTTT TTCCCAGCTG CTACTGGGAT TCTTGCTGGT 
GCCAACATCT CTGGAGACCT GGAGGATCCC CAGGACGCCA TCCCCAGAGG GACCATGCTG 
GCCATCTTCA TCACCACCGT GGCCTACATA GGTGTTGCTA TTTGCGTAGC CGCCTGTGTG 
GTCCGAGATG CCACTGGCAG CATGAATGAC ACCATCGTTT CTGGGATGAA TTGCAATGGC 
TCTGCGGCCT GTGGGTTGGG CTACGACTTC TCAAGATGTC AACACGAGCC ATGTCAGTAT 
GGGCTGATGA ACAATTTCCA GGTCATGAGC ATGGTGTCCG GGTTCGGGCC CCTCATCACT 
GCAGGGATCT TTTCGGCAAC ACTGTCTTCC GCCCTGGCCT CCCTCGTCAG TGCGCCCAAA 
GTATTCCAGG CTCTGTGCAA GGACAACATC TTCAAAGGAC TGCAGTTCTT CGCAAAGGGC 
TATGGGAAAA ACAACGAGCC CCTGAGAGGG TACTTCCTCA CTTTTGTGAT AGCCATGGCA 
TTCATTCTCA TCGCCGAGCT GAATGTCATT GCCCCCATCA TCTCCAACTT CTTCCTGGCC 
TCATATGCGC TTATTAATTT CTCCTGTTTC CATGCCTCGT ATGCCAAATC TCCAGGATGG 
AGGCCTGCAT ATGGGATTTA CAACATGTGG GTATCTCTTT TTGGAGCGAT TTTGTGCTGT 
GCAGTCATGT TTGTCATCAA CTGGTGGGCA GCTGTCATCA CTTATGTCAT TGAGTTATTC 
CTCTATATCT ATGTGACTTA TAAGAAGCCA GATGTGAACT GGGGCTCCTC CACACAGGCT 
CTGTCCTATG TGAGTGCTTT AGACAACGCT CTGGAATTAA CCACAGTGGA AGACCATGTG 
AAAAACTTCA GACCCCAGTG CATTGTCTTA ACAGGCGGAC CCATGACTAG ACCCGCTCTC 
TTGGATATAA CCCACGCCTT TACGAAGAAT AGTGGCCTCT GCATCTGCTG CGAAGTCTTC 
GTGGGACCTC GCAAGCTGTG TGTTAAAGAG ATGAACAGCG GCATGGCTAA AAAACAGGCC 
TGGCTTATAA AGAACAAAAT CAAAGCTTTT TATGCTGCAG TGGCTGCAGA CTGCTTCAGG 
GACGGTGTCC GGAGCCTCCT TCAGGCCTCA GGCTTGGGGA GAATGAAGCC AAACACTCTC 
GTGATTGGAT ACAAAAAGAA CTGGAGGAAA GCTCCCTTGT CAGAGCTGGA GAACTACGTG 
GGAATCATAC ATGACGCCTT TGACTTTGAG ATTGGCGTGG TCATAGTCAG AATCAGCCAA 
GGCTTTGACA TCTCTCCGGT TCTCCAGGTA CAAGACGAAC TGGAGAAACT GGAACAGGAG 
AGATTGGCTC TGGAGGCCGC AATCAAAGAC AACGAGTGTG AGGAGGGCAA GGGGGGCATC 
CGAGGCTTGT TTAAGAAGGC CGGCAAGCTG AACATTACCA AGCCAGCCCC CAAGAAAGAC 
GGCAACATCA GCAGCATCCA GTCAATGCAC GTCGGGGAGT TCAATCAGAA ACTGGTGGAA 
GCCAGCGCTC AGTTTAAAAA GAAGCAGGGA AAAGGCACGA TTGATGTTTG GTGGTTATTT 
GATGATGGAG GGTTAACACT GCTTATCCCA TATATCTTGA CTCTCAGAAA AAAATGGAAA 
GATTGTAAGT TAAGAATCTA TGTTGGAGGA AAGATCAACC GCATCGAAGA GGAAAAAATT 
TCAATGGCTT CACTTCTCAG CAAATTTAGA ATAAAATTTG CAGACATCCA TATAATTGGA 
GACATCAACA TTAAGCCAAA CAAAGAGAGC TGGAAAGTCT TTGAAGAGAT GATTGAACCT 
TATCGGCTTC ATGAAAGCCA CAAAGATTTG ACCACTGCTG AGAAATTAAA AAGAGAAAGT 
CCATGGAAAA TTACAGATGC AGAACTGGAA GCAGTCAAGG AAAAGAGTTA CCGTCAAGTT 
CGACTGAACG AGCTCTTACA GGAACACTCC AGAGCCGCCA ACCTCATCGT GTTGAGCCTT 
CCAGTGGCAA GAAAAGGATC TATTTCGGAT TTGTTGTACA TGGCGTGGTT AGAAATCCTG 
ACCAAGAACC TTCCTCCTGT GTTACTGGTT AGAGGAAATC ACAAAAATGT CTTAACATTT 
TACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248888.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104748.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu87768
Clone ID Related Accession (Same CDS sequence)	XM_030248888.1
Accession Version	XM_030248888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTC TTGGAGTTCT TATAATTCTT 
CTTTCCACCA TGGTAACCTC TATCACTGGG TTGTCAACTT CTGCAATAGC AACAAACGGG 
TTTGTTCGTG GAGGTGGAGC CTACTACCTG ATTTCCCGGA GCTTAGGGCC TGAGTTCGGT 
GGGTCAATAG GCTTGATCTT TGCTTTTGCC AATGCAGTGG CTGTCGCTAT GTATGTGGTG 
GGATTCGCGG AGACTGTCGT GGATCTTCTT AAGGAGAGCG ATTCAATGAT GGTGGATCCA 
ACCAATGACA TCCGCATCAT CGGTTCCATC ACCGTGGTGA TTCTTCTAGG AATCTCAGTA 
GCTGGGATGG AGTGGGAAGC AAAGGCTCAA GTCATTCTCC TGGTCATTCT TCTCATTGCC 
ATTGCAAACT TCTTCATTGG AACTGTCATC CCATCCAACA ATGAGAAGAA ATCCAGAGGC 
TTCTTTAACT ACCAAGCATC TATATTTGCG GAAAACTTTG GGCCAAGCTT CACAAAGGGG 
GAAGGCTTCT TCTCGGTCTT TGCCATCTTT TTCCCAGCTG CTACTGGGAT TCTTGCTGGT 
GCCAACATCT CTGGAGACCT GGAGGATCCC CAGGACGCCA TCCCCAGAGG GACCATGCTG 
GCCATCTTCA TCACCACCGT GGCCTACATA GGTGTTGCTA TTTGCGTAGC CGCCTGTGTG 
GTCCGAGATG CCACTGGCAG CATGAATGAC ACCATCGTTT CTGGGATGAA TTGCAATGGC 
TCTGCGGCCT GTGGGTTGGG CTACGACTTC TCAAGATGTC AACACGAGCC ATGTCAGTAT 
GGGCTGATGA ACAATTTCCA GGTCATGAGC ATGGTGTCCG GGTTCGGGCC CCTCATCACT 
GCAGGGATCT TTTCGGCAAC ACTGTCTTCC GCCCTGGCCT CCCTCGTCAG TGCGCCCAAA 
GTATTCCAGG CTCTGTGCAA GGACAACATC TTCAAAGGAC TGCAGTTCTT CGCAAAGGGC 
TATGGGAAAA ACAACGAGCC CCTGAGAGGG TACTTCCTCA CTTTTGTGAT AGCCATGGCA 
TTCATTCTCA TCGCCGAGCT GAATGTCATT GCCCCCATCA TCTCCAACTT CTTCCTGGCC 
TCATATGCGC TTATTAATTT CTCCTGTTTC CATGCCTCGT ATGCCAAATC TCCAGGATGG 
AGGCCTGCAT ATGGGATTTA CAACATGTGG GTATCTCTTT TTGGAGCGAT TTTGTGCTGT 
GCAGTCATGT TTGTCATCAA CTGGTGGGCA GCTGTCATCA CTTATGTCAT TGAGTTATTC 
CTCTATATCT ATGTGACTTA TAAGAAGCCA GATGTGAACT GGGGCTCCTC CACACAGGCT 
CTGTCCTATG TGAGTGCTTT AGACAACGCT CTGGAATTAA CCACAGTGGA AGACCATGTG 
AAAAACTTCA GACCCCAGTG CATTGTCTTA ACAGGCGGAC CCATGACTAG ACCCGCTCTC 
TTGGATATAA CCCACGCCTT TACGAAGAAT AGTGGCCTCT GCATCTGCTG CGAAGTCTTC 
GTGGGACCTC GCAAGCTGTG TGTTAAAGAG ATGAACAGCG GCATGGCTAA AAAACAGGCC 
TGGCTTATAA AGAACAAAAT CAAAGCTTTT TATGCTGCAG TGGCTGCAGA CTGCTTCAGG 
GACGGTGTCC GGAGCCTCCT TCAGGCCTCA GGCTTGGGGA GAATGAAGCC AAACACTCTC 
GTGATTGGAT ACAAAAAGAA CTGGAGGAAA GCTCCCTTGT CAGAGCTGGA GAACTACGTG 
GGAATCATAC ATGACGCCTT TGACTTTGAG ATTGGCGTGG TCATAGTCAG AATCAGCCAA 
GGCTTTGACA TCTCTCCGGT TCTCCAGGTA CAAGACGAAC TGGAGAAACT GGAACAGGAG 
AGATTGGCTC TGGAGGCCGC AATCAAAGAC AACGAGTGTG AGGAGGGCAA GGGGGGCATC 
CGAGGCTTGT TTAAGAAGGC CGGCAAGCTG AACATTACCA AGCCAGCCCC CAAGAAAGAC 
GGCAACATCA GCAGCATCCA GTCAATGCAC GTCGGGGAGT TCAATCAGAA ACTGGTGGAA 
GCCAGCGCTC AGTTTAAAAA GAAGCAGGGA AAAGGCACGA TTGATGTTTG GTGGTTATTT 
GATGATGGAG GGTTAACACT GCTTATCCCA TATATCTTGA CTCTCAGAAA AAAATGGAAA 
GATTGTAAGT TAAGAATCTA TGTTGGAGGA AAGATCAACC GCATCGAAGA GGAAAAAATT 
TCAATGGCTT CACTTCTCAG CAAATTTAGA ATAAAATTTG CAGACATCCA TATAATTGGA 
GACATCAACA TTAAGCCAAA CAAAGAGAGC TGGAAAGTCT TTGAAGAGAT GATTGAACCT 
TATCGGCTTC ATGAAAGCCA CAAAGATTTG ACCACTGCTG AGAAATTAAA AAGAGAAAGT 
CCATGGAAAA TTACAGATGC AGAACTGGAA GCAGTCAAGG AAAAGAGTTA CCGTCAAGTT 
CGACTGAACG AGCTCTTACA GGAACACTCC AGAGCCGCCA ACCTCATCGT GTTGAGCCTT 
CCAGTGGCAA GAAAAGGATC TATTTCGGAT TTGTTGTACA TGGCGTGGTT AGAAATCCTG 
ACCAAGAACC TTCCTCCTGT GTTACTGGTT AGAGGAAATC ACAAAAATGT CTTAACATTT 
TACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104748.1
CDS	323..3691
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248888.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248888.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTC TTGGAGTTCT TATAATTCTT 
CTTTCCACCA TGGTAACCTC TATCACTGGG TTGTCAACTT CTGCAATAGC AACAAACGGG 
TTTGTTCGTG GAGGTGGAGC CTACTACCTG ATTTCCCGGA GCTTAGGGCC TGAGTTCGGT 
GGGTCAATAG GCTTGATCTT TGCTTTTGCC AATGCAGTGG CTGTCGCTAT GTATGTGGTG 
GGATTCGCGG AGACTGTCGT GGATCTTCTT AAGGAGAGCG ATTCAATGAT GGTGGATCCA 
ACCAATGACA TCCGCATCAT CGGTTCCATC ACCGTGGTGA TTCTTCTAGG AATCTCAGTA 
GCTGGGATGG AGTGGGAAGC AAAGGCTCAA GTCATTCTCC TGGTCATTCT TCTCATTGCC 
ATTGCAAACT TCTTCATTGG AACTGTCATC CCATCCAACA ATGAGAAGAA ATCCAGAGGC 
TTCTTTAACT ACCAAGCATC TATATTTGCG GAAAACTTTG GGCCAAGCTT CACAAAGGGG 
GAAGGCTTCT TCTCGGTCTT TGCCATCTTT TTCCCAGCTG CTACTGGGAT TCTTGCTGGT 
GCCAACATCT CTGGAGACCT GGAGGATCCC CAGGACGCCA TCCCCAGAGG GACCATGCTG 
GCCATCTTCA TCACCACCGT GGCCTACATA GGTGTTGCTA TTTGCGTAGC CGCCTGTGTG 
GTCCGAGATG CCACTGGCAG CATGAATGAC ACCATCGTTT CTGGGATGAA TTGCAATGGC 
TCTGCGGCCT GTGGGTTGGG CTACGACTTC TCAAGATGTC AACACGAGCC ATGTCAGTAT 
GGGCTGATGA ACAATTTCCA GGTCATGAGC ATGGTGTCCG GGTTCGGGCC CCTCATCACT 
GCAGGGATCT TTTCGGCAAC ACTGTCTTCC GCCCTGGCCT CCCTCGTCAG TGCGCCCAAA 
GTATTCCAGG CTCTGTGCAA GGACAACATC TTCAAAGGAC TGCAGTTCTT CGCAAAGGGC 
TATGGGAAAA ACAACGAGCC CCTGAGAGGG TACTTCCTCA CTTTTGTGAT AGCCATGGCA 
TTCATTCTCA TCGCCGAGCT GAATGTCATT GCCCCCATCA TCTCCAACTT CTTCCTGGCC 
TCATATGCGC TTATTAATTT CTCCTGTTTC CATGCCTCGT ATGCCAAATC TCCAGGATGG 
AGGCCTGCAT ATGGGATTTA CAACATGTGG GTATCTCTTT TTGGAGCGAT TTTGTGCTGT 
GCAGTCATGT TTGTCATCAA CTGGTGGGCA GCTGTCATCA CTTATGTCAT TGAGTTATTC 
CTCTATATCT ATGTGACTTA TAAGAAGCCA GATGTGAACT GGGGCTCCTC CACACAGGCT 
CTGTCCTATG TGAGTGCTTT AGACAACGCT CTGGAATTAA CCACAGTGGA AGACCATGTG 
AAAAACTTCA GACCCCAGTG CATTGTCTTA ACAGGCGGAC CCATGACTAG ACCCGCTCTC 
TTGGATATAA CCCACGCCTT TACGAAGAAT AGTGGCCTCT GCATCTGCTG CGAAGTCTTC 
GTGGGACCTC GCAAGCTGTG TGTTAAAGAG ATGAACAGCG GCATGGCTAA AAAACAGGCC 
TGGCTTATAA AGAACAAAAT CAAAGCTTTT TATGCTGCAG TGGCTGCAGA CTGCTTCAGG 
GACGGTGTCC GGAGCCTCCT TCAGGCCTCA GGCTTGGGGA GAATGAAGCC AAACACTCTC 
GTGATTGGAT ACAAAAAGAA CTGGAGGAAA GCTCCCTTGT CAGAGCTGGA GAACTACGTG 
GGAATCATAC ATGACGCCTT TGACTTTGAG ATTGGCGTGG TCATAGTCAG AATCAGCCAA 
GGCTTTGACA TCTCTCCGGT TCTCCAGGTA CAAGACGAAC TGGAGAAACT GGAACAGGAG 
AGATTGGCTC TGGAGGCCGC AATCAAAGAC AACGAGTGTG AGGAGGGCAA GGGGGGCATC 
CGAGGCTTGT TTAAGAAGGC CGGCAAGCTG AACATTACCA AGCCAGCCCC CAAGAAAGAC 
GGCAACATCA GCAGCATCCA GTCAATGCAC GTCGGGGAGT TCAATCAGAA ACTGGTGGAA 
GCCAGCGCTC AGTTTAAAAA GAAGCAGGGA AAAGGCACGA TTGATGTTTG GTGGTTATTT 
GATGATGGAG GGTTAACACT GCTTATCCCA TATATCTTGA CTCTCAGAAA AAAATGGAAA 
GATTGTAAGT TAAGAATCTA TGTTGGAGGA AAGATCAACC GCATCGAAGA GGAAAAAATT 
TCAATGGCTT CACTTCTCAG CAAATTTAGA ATAAAATTTG CAGACATCCA TATAATTGGA 
GACATCAACA TTAAGCCAAA CAAAGAGAGC TGGAAAGTCT TTGAAGAGAT GATTGAACCT 
TATCGGCTTC ATGAAAGCCA CAAAGATTTG ACCACTGCTG AGAAATTAAA AAGAGAAAGT 
CCATGGAAAA TTACAGATGC AGAACTGGAA GCAGTCAAGG AAAAGAGTTA CCGTCAAGTT 
CGACTGAACG AGCTCTTACA GGAACACTCC AGAGCCGCCA ACCTCATCGT GTTGAGCCTT 
CCAGTGGCAA GAAAAGGATC TATTTCGGAT TTGTTGTACA TGGCGTGGTT AGAAATCCTG 
ACCAAGAACC TTCCTCCTGT GTTACTGGTT AGAGGAAATC ACAAAAATGT CTTAACATTT 
TACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248891.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104751.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X7, mRNA.

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CloneID	OMu87769
Clone ID Related Accession (Same CDS sequence)	XM_030248891.1 , XM_030248890.1
Accession Version	XM_030248891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104751.1
CDS	271..3543
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248891.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248891.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_030248890.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104750.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X6, mRNA.

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CloneID	OMu87769
Clone ID Related Accession (Same CDS sequence)	XM_030248891.1 , XM_030248890.1
Accession Version	XM_030248890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104750.1
CDS	323..3595
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_030248890.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248890.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACCAT GGACGCCGTC 
CCCAAGATTG AATACTACCG CAACACAGGC AGCGTGAGCG GGCCCAAGGT CAACCGGCCC 
AGCCTGCTGG AGATCCACGA GCAGCTGGCT AAGAATGTGA CTGTGGCCCC TGGTTCAGCT 
GACAGGGTTG CTAATGGAGA TGGGATGCCT GGAGATGAGC AAGCAGAAAA CAAGGAAGAA 
GACATGACTG GAGTTGTGAA GTTTGGATGG GTGAAAGGTG TGCTGGTGCG ATGCATGCTG 
AACATCTGGG GAGTCATGCT CTTCATTCGC CTCTCCTGGA TTGTAGGAGA AGCGGGAATT 
GGTCTCGGTG TGATTATCAT CGGCTTAGCC GTGACAGTGA CAGCCATCAC AGGTTTGTCC 
ACCTCTGCTA TCGCCACAAA CGGATATGTC AGAGGAGGTG GAGCCTACTA CCTGATTTCC 
CGGAGCTTAG GGCCTGAGTT CGGTGGGTCA ATAGGCTTGA TCTTTGCTTT TGCCAATGCA 
GTGGCTGTCG CTATGTATGT GGTGGGATTC GCGGAGACTG TCGTGGATCT TCTTAAGGAG 
AGCGATTCAA TGATGGTGGA TCCAACCAAT GACATCCGCA TCATCGGTTC CATCACCGTG 
GTGATTCTTC TAGGAATCTC AGTAGCTGGG ATGGAGTGGG AAGCAAAGGC TCAAGTCATT 
CTCCTGGTCA TTCTTCTCAT TGCCATTGCA AACTTCTTCA TTGGAACTGT CATCCCATCC 
AACAATGAGA AGAAATCCAG AGGCTTCTTT AACTACCAAG CATCTATATT TGCGGAAAAC 
TTTGGGCCAA GCTTCACAAA GGGGGAAGGC TTCTTCTCGG TCTTTGCCAT CTTTTTCCCA 
GCTGCTACTG GGATTCTTGC TGGTGCCAAC ATCTCTGGAG ACCTGGAGGA TCCCCAGGAC 
GCCATCCCCA GAGGGACCAT GCTGGCCATC TTCATCACCA CCGTGGCCTA CATAGGTGTT 
GCTATTTGCG TAGCCGCCTG TGTGGTCCGA GATGCCACTG GCAGCATGAA TGACACCATC 
GTTTCTGGGA TGAATTGCAA TGGCTCTGCG GCCTGTGGGT TGGGCTACGA CTTCTCAAGA 
TGTCAACACG AGCCATGTCA GTATGGGCTG ATGAACAATT TCCAGGTCAT GAGCATGGTG 
TCCGGGTTCG GGCCCCTCAT CACTGCAGGG ATCTTTTCGG CAACACTGTC TTCCGCCCTG 
GCCTCCCTCG TCAGTGCGCC CAAAGTATTC CAGGCTCTGT GCAAGGACAA CATCTTCAAA 
GGACTGCAGT TCTTCGCAAA GGGCTATGGG AAAAACAACG AGCCCCTGAG AGGGTACTTC 
CTCACTTTTG TGATAGCCAT GGCATTCATT CTCATCGCCG AGCTGAATGT CATTGCCCCC 
ATCATCTCCA ACTTCTTCCT GGCCTCATAT GCGCTTATTA ATTTCTCCTG TTTCCATGCC 
TCGTATGCCA AATCTCCAGG ATGGAGGCCT GCATATGGGA TTTACAACAT GTGGGTATCT 
CTTTTTGGAG CGATTTTGTG CTGTGCAGTC ATGTTTGTCA TCAACTGGTG GGCAGCTGTC 
ATCACTTATG TCATTGAGTT ATTCCTCTAT ATCTATGTGA CTTATAAGAA GCCAGATGTG 
AACTGGGGCT CCTCCACACA GGCTCTGTCC TATGTGAGTG CTTTAGACAA CGCTCTGGAA 
TTAACCACAG TGGAAGACCA TGTGAAAAAC TTCAGACCCC AGTGCATTGT CTTAACAGGC 
GGACCCATGA CTAGACCCGC TCTCTTGGAT ATAACCCACG CCTTTACGAA GAATAGTGGC 
CTCTGCATCT GCTGCGAAGT CTTCGTGGGA CCTCGCAAGC TGTGTGTTAA AGAGATGAAC 
AGCGGCATGG CTAAAAAACA GGCCTGGCTT ATAAAGAACA AAATCAAAGC TTTTTATGCT 
GCAGTGGCTG CAGACTGCTT CAGGGACGGT GTCCGGAGCC TCCTTCAGGC CTCAGGCTTG 
GGGAGAATGA AGCCAAACAC TCTCGTGATT GGATACAAAA AGAACTGGAG GAAAGCTCCC 
TTGTCAGAGC TGGAGAACTA CGTGGGAATC ATACATGACG CCTTTGACTT TGAGATTGGC 
GTGGTCATAG TCAGAATCAG CCAAGGCTTT GACATCTCTC CGGTTCTCCA GGTACAAGAC 
GAACTGGAGA AACTGGAACA GGAGAGATTG GCTCTGGAGG CCGCAATCAA AGACAACGAG 
TGTGAGGAGG GCAAGGGGGG CATCCGAGGC TTGTTTAAGA AGGCCGGCAA GCTGAACATT 
ACCAAGCCAG CCCCCAAGAA AGACGGCAAC ATCAGCAGCA TCCAGTCAAT GCACGTCGGG 
GAGTTCAATC AGAAACTGGT GGAAGCCAGC GCTCAGTTTA AAAAGAAGCA GGGAAAAGGC 
ACGATTGATG TTTGGTGGTT ATTTGATGAT GGAGGGTTAA CACTGCTTAT CCCATATATC 
TTGACTCTCA GAAAAAAATG GAAAGATTGT AAGTTAAGAA TCTATGTTGG AGGAAAGATC 
AACCGCATCG AAGAGGAAAA AATTTCAATG GCTTCACTTC TCAGCAAATT TAGAATAAAA 
TTTGCAGACA TCCATATAAT TGGAGACATC AACATTAAGC CAAACAAAGA GAGCTGGAAA 
GTCTTTGAAG AGATGATTGA ACCTTATCGG CTTCATGAAA GCCACAAAGA TTTGACCACT 
GCTGAGAAAT TAAAAAGAGA AAGTCCATGG AAAATTACAG ATGCAGAACT GGAAGCAGTC 
AAGGAAAAGA GTTACCGTCA AGTTCGACTG AACGAGCTCT TACAGGAACA CTCCAGAGCC 
GCCAACCTCA TCGTGTTGAG CCTTCCAGTG GCAAGAAAAG GATCTATTTC GGATTTGTTG 
TACATGGCGT GGTTAGAAAT CCTGACCAAG AACCTTCCTC CTGTGTTACT GGTTAGAGGA 
AATCACAAAA ATGTCTTAAC ATTTTACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( NM_001079690.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001073158.1 Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20761
Clone ID Related Accession (Same CDS sequence)	NM_001079690.1
Accession Version	NM_001079690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 1 isoform F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052750.1, AK128928.1, U20973.1, AL929451.8, AI527944.1, AI875508.1 and AL844547.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U20975.1, U94518.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACTTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAATAC TACCGCAACA CAGGCAGCGT GAGCGGGCCC 
AAGGTCAACC GGCCCAGCCT GCTGGAGATC CACGAGCAGC TGGCTAAGAA TGTGACTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACAT GACTGGAGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGCGATGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT GGGCGTCATC ATCATTGGCC TGAGCGTAGT TGTGACAACA 
CTCACAGGTA TTTCTATGTC GGCTATTTGC ACAAACGGAG TGGTGCGAGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATCTTT 
GCTTTTGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GATTCGCGGA GACTGTCGTG 
GATCTTCTTA AGGAGAGCGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGCATCATC 
GGTTCCATCA CCGTGGTGAT TCTTCTAGGA ATCTCAGTAG CTGGGATGGA GTGGGAAGCA 
AAGGCTCAAG TCATTCTCCT GGTCATTCTT CTCATTGCCA TTGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA TGAGAAGAAA TCCAGAGGCT TCTTTAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAAAGGGGG AAGGCTTCTT CTCGGTCTTT 
GCCATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATCTTCAT CACCACCGTG 
GCCTACATAG GTGTTGCTAT TTGCGTAGCC GCCTGTGTGG TCCGAGATGC CACTGGCAGC 
ATGAATGACA CCATCGTTTC TGGGATGAAT TGCAATGGCT CTGCGGCCTG TGGGTTGGGC 
TACGACTTCT CAAGATGTCA ACACGAGCCA TGTCAGTATG GGCTGATGAA CAATTTCCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGGCCC CTCATCACTG CAGGGATCTT TTCGGCAACA 
CTGTCTTCCG CCCTGGCCTC CCTCGTCAGT GCGCCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT GCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AATGTCATTG CCCCCATCAT CTCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGTTTCC ATGCCTCGTA TGCCAAATCT CCAGGATGGA GGCCTGCATA TGGGATTTAC 
AACATGTGGG TATCTCTTTT TGGAGCGATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCAG CTGTCATCAC TTATGTCATT GAGTTATTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTATGT GAGTGCTTTA 
GACAACGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGCGGACC CATGACTAGA CCCGCTCTCT TGGATATAAC CCACGCCTTT 
ACGAAGAATA GTGGCCTCTG CATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGCGG CATGGCTAAA AAACAGGCCT GGCTTATAAA GAACAAAATC 
AAAGCTTTTT ATGCTGCAGT GGCTGCAGAC TGCTTCAGGG ACGGTGTCCG GAGCCTCCTT 
CAGGCCTCAG GCTTGGGGAG AATGAAGCCA AACACTCTCG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC AGAGCTGGAG AACTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGCGTGGT CATAGTCAGA ATCAGCCAAG GCTTTGACAT CTCTCCGGTT 
CTCCAGGTAC AAGACGAACT GGAGAAACTG GAACAGGAGA GATTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGAGTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAGCTGA ACATTACCAA GCCAGCCCCC AAGAAAGACG GCAACATCAG CAGCATCCAG 
TCAATGCACG TCGGGGAGTT CAATCAGAAA CTGGTGGAAG CCAGCGCTCA GTTTAAAAAG 
AAGCAGGGAA AAGGCACGAT TGATGTTTGG TGGTTATTTG ATGATGGAGG GTTAACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAA AAATGGAAAG ATTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AGATCAACCG CATCGAAGAG GAAAAAATTT CAATGGCTTC ACTTCTCAGC 
AAATTTAGAA TAAAATTTGC AGACATCCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATCGGCTTCA TGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGTCAAGTTC GACTGAACGA GCTCTTACAG 
GAACACTCCA GAGCCGCCAA CCTCATCGTG TTGAGCCTTC CAGTGGCAAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT TCCTCCTGTG 
TTACTGGTTA GAGGAAATCA CAAAAATGTC TTAACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073158.1
CDS	233..3520
Translation	MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEETSFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_001079690.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 1 (Slc12a1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079690.1

ORF Insert Sequence:

ATGTCGGTCA GCATCCCTTC CAACTCAGTG CCCAGTAGTG CCAGTCGCTT TCAGGTCCAT 
GTTATAAACG AGGGCCATGG CAGTGCGGCA GCCGTGGGCG ACAGTGCAGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGACGAAGCC CAAAACAGAC TGAGAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA CAATTTTCTC CAAACTGGAG AGACTGCCAA AACAGACACC 
ACGTTTCACG CCTACGACTC TCACACAAAC ACATACTACC TACAAACTTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAATAC TACCGCAACA CAGGCAGCGT GAGCGGGCCC 
AAGGTCAACC GGCCCAGCCT GCTGGAGATC CACGAGCAGC TGGCTAAGAA TGTGACTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACAT GACTGGAGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGCGATGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT GGGCGTCATC ATCATTGGCC TGAGCGTAGT TGTGACAACA 
CTCACAGGTA TTTCTATGTC GGCTATTTGC ACAAACGGAG TGGTGCGAGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATCTTT 
GCTTTTGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GATTCGCGGA GACTGTCGTG 
GATCTTCTTA AGGAGAGCGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGCATCATC 
GGTTCCATCA CCGTGGTGAT TCTTCTAGGA ATCTCAGTAG CTGGGATGGA GTGGGAAGCA 
AAGGCTCAAG TCATTCTCCT GGTCATTCTT CTCATTGCCA TTGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA TGAGAAGAAA TCCAGAGGCT TCTTTAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAAAGGGGG AAGGCTTCTT CTCGGTCTTT 
GCCATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATCTTCAT CACCACCGTG 
GCCTACATAG GTGTTGCTAT TTGCGTAGCC GCCTGTGTGG TCCGAGATGC CACTGGCAGC 
ATGAATGACA CCATCGTTTC TGGGATGAAT TGCAATGGCT CTGCGGCCTG TGGGTTGGGC 
TACGACTTCT CAAGATGTCA ACACGAGCCA TGTCAGTATG GGCTGATGAA CAATTTCCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGGCCC CTCATCACTG CAGGGATCTT TTCGGCAACA 
CTGTCTTCCG CCCTGGCCTC CCTCGTCAGT GCGCCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT GCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AATGTCATTG CCCCCATCAT CTCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGTTTCC ATGCCTCGTA TGCCAAATCT CCAGGATGGA GGCCTGCATA TGGGATTTAC 
AACATGTGGG TATCTCTTTT TGGAGCGATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCAG CTGTCATCAC TTATGTCATT GAGTTATTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTATGT GAGTGCTTTA 
GACAACGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGCGGACC CATGACTAGA CCCGCTCTCT TGGATATAAC CCACGCCTTT 
ACGAAGAATA GTGGCCTCTG CATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGCGG CATGGCTAAA AAACAGGCCT GGCTTATAAA GAACAAAATC 
AAAGCTTTTT ATGCTGCAGT GGCTGCAGAC TGCTTCAGGG ACGGTGTCCG GAGCCTCCTT 
CAGGCCTCAG GCTTGGGGAG AATGAAGCCA AACACTCTCG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC AGAGCTGGAG AACTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGCGTGGT CATAGTCAGA ATCAGCCAAG GCTTTGACAT CTCTCCGGTT 
CTCCAGGTAC AAGACGAACT GGAGAAACTG GAACAGGAGA GATTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGAGTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAGCTGA ACATTACCAA GCCAGCCCCC AAGAAAGACG GCAACATCAG CAGCATCCAG 
TCAATGCACG TCGGGGAGTT CAATCAGAAA CTGGTGGAAG CCAGCGCTCA GTTTAAAAAG 
AAGCAGGGAA AAGGCACGAT TGATGTTTGG TGGTTATTTG ATGATGGAGG GTTAACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAA AAATGGAAAG ATTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AGATCAACCG CATCGAAGAG GAAAAAATTT CAATGGCTTC ACTTCTCAGC 
AAATTTAGAA TAAAATTTGC AGACATCCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATCGGCTTCA TGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGTCAAGTTC GACTGAACGA GCTCTTACAG 
GAACACTCCA GAGCCGCCAA CCTCATCGTG TTGAGCCTTC CAGTGGCAAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT TCCTCCTGTG 
TTACTGGTTA GAGGAAATCA CAAAAATGTC TTAACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Slc12a1
Entrez Gene ID	20495
Full Name	solute carrier family 12, member 1
Synonyms	AI788571,D630042G03Rik,Nkcc2,mBSC1,urehr3
General protein information	Preferred Names solute carrier family 12, member 1 Names solute carrier family 12 member 1 BSC1 bumetanide-sensitive cotransporter type 1 bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 kidney-specific Na-K-Cl symporter
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 F1\|2 61.23 cM

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Slc12a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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