SLC12A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A1
Entrez Gene ID	6557
Full Name	solute carrier family 12 member 1
Synonyms	BSC1,NKCC2
General protein information	Preferred Names solute carrier family 12 member 1 Names solute carrier family 12 member 1 NKCC2A variant A Na-K-2Cl cotransporter bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 kidney-specific Na-K-Cl symporter solute carrier family 12 (sodium/potassium/chloride transporter), member 1 solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.1
Summary	This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010].
Disorder MIM:	600839
Disorder Html:	Bartter syndrome, type 1, 601678 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000338.2	NP_000329.2	solute carrier family 12 member 1 isoform A
NM_001184832.2	NP_001171761.1	solute carrier family 12 member 1 isoform F
NM_000338.3	NP_000329.2	solute carrier family 12 member 1 isoform A
XM_005254606.1	XP_005254663.1	solute carrier family 12 member 1 isoform X1
NM_001184832.1	NP_001171761.1	solute carrier family 12 member 1 isoform F
XM_005254606.2	XP_005254663.1	solute carrier family 12 member 1 isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel SLC12A1 gene mutation associated with hyperparathyroidism, hypercalcemia, nephrogenic diabetes insipidus, and nephrocalcinosis in four patients.
Wongsaengsak S, Vidmar AP, Addala A, Kamil ES, Sequeira P, Fass B, Pitukcheewanont P
Bone97121-125(2017 04)

A novel variant in the SLC12A1 gene in two families with antenatal Bartter syndrome.
Breinbjerg A, Siggaard Rittig C, Gregersen N, Rittig S, Hvarregaard Christensen J
Acta paediatrica (Oslo, Norway : 1992)106(1)161-167(2017 Jan)

Antenatal Bartter syndrome presenting as hyperparathyroidism with hypercalcemia and hypercalciuria: a case report and review.
Gross I, Siedner-Weintraub Y, Simckes A, Gillis D
Journal of pediatric endocrinology & metabolism : JPEM28(7-8)943-6(2015 Jul)

Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and functional significance.
Castrop H, Schnermann J
American journal of physiology. Renal physiology295(4)F859-66(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an association between primary hyperparathyroidism and loss of function mutation of SLC12A1, which may result in an aberrant threshold of the calcium sensing receptor at the level of the kidney, is reported.
Title: A novel SLC12A1 gene mutation associated with hyperparathyroidism, hypercalcemia, nephrogenic diabetes insipidus, and nephrocalcinosis in four patients.

A novel variant in the SLC12A1 gene, c.1614T>A, which predicts a change from a tyrosine codon to a stop codon (p.Tyr538Ter) was found in two families with Bartter syndrome type I.
Title: A novel variant in the SLC12A1 gene in two families with antenatal Bartter syndrome.

Mutations in SLC12A1 gene is associated with Bartter syndrome.
Title: Antenatal Bartter syndrome presenting as hyperparathyroidism with hypercalcemia and hypercalciuria: a case report and review.

Low SLC12A1 urine levels were associated with Bartter syndrome.
Title: Urinary exosomes in the diagnosis of Gitelman and Bartter syndromes.

The association between polymorphisms in KCNJ1, SLC12A1, and 7 other genes and calcium intake and colorectal neoplasia risk was studied.
Title: Calcium intake and ion transporter genetic polymorphisms interact in human colorectal neoplasia risk in a 2-phase study.

The following SLC12A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25763	NM_000338.3 Latest version!	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu21575	NM_001184832.2 Latest version!	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu48511	XM_005254606.2 Latest version!	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu25763	NM_000338.2	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu21575	NM_001184832.1	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu48511	XM_005254606.1	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

SLC12A1 ( NM_000338.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000329.2 Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25763
Clone ID Related Accession (Same CDS sequence)	NM_000338.2 , NM_000338.3
Accession Version	NM_000338.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA632985.1, BC040138.2, U58130.1, AC023355.5 and AA917702.1. This sequence is a reference standard in the RefSeqGene project. On Mar 29, 2007 this sequence version replaced NM_000338.1. Transcript Variant: This variant (1) encodes isoform A. Isoforms A and F are the same length but differ in the region of amino acids 214-238. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: EF559316.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTAACTT CTATTACTGG GTTGTCAACT TCTGCGATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000329.2
CDS	217..3516
Translation	MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_000338.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000338.2

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTAACTT CTATTACTGG GTTGTCAACT TCTGCGATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( NM_000338.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000329.2 Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25763
Clone ID Related Accession (Same CDS sequence)	NM_000338.2 , NM_000338.3
Accession Version	NM_000338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA632985.1, BC040138.2, U58130.1, AC023355.5 and AA917702.1. This sequence is a reference standard in the RefSeqGene project. On Jul 25, 2019 this sequence version replaced NM_000338.2. Transcript Variant: This variant (1) encodes isoform A. Isoforms A and F are the same length but differ in the region of amino acids 214-238. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: EF559316.2 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTAACTT CTATTACTGG GTTGTCAACT TCTGCGATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000329.2
CDS	216..3515
Translation	MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_000338.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000338.3

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTAACTT CTATTACTGG GTTGTCAACT TCTGCGATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( NM_001184832.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171761.1 Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 2, mRNA.

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CloneID	OHu21575
Clone ID Related Accession (Same CDS sequence)	NM_001184832.2 , NM_001184832.1
Accession Version	NM_001184832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 1 isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA632985.1, AK298312.1, U58130.1, AC023355.5 and AA917702.1. On Jun 2, 2019 this sequence version replaced NM_001184832.1. Transcript Variant: This variant (2) uses an alternate in-frame exon in the 5' coding region, compared to variant 1, and encodes isoform F. Isoforms A and F are the same length but differ in the region of amino acids 214-238. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U58130.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTCA TCATCATTGG CCTAAGTGTG 
GTAGTAACGA CACTCACAGG TATTTCTATG TCTGCTATTT GCACGAATGG AGTAGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171761.1
CDS	216..3515
Translation	MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_001184832.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184832.2

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTCA TCATCATTGG CCTAAGTGTG 
GTAGTAACGA CACTCACAGG TATTTCTATG TCTGCTATTT GCACGAATGG AGTAGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( NM_001184832.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171761.1 Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 2, mRNA.

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CloneID	OHu21575
Clone ID Related Accession (Same CDS sequence)	NM_001184832.2 , NM_001184832.1
Accession Version	NM_001184832.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 1 isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA632985.1, AK298312.1, U58130.1, AC023355.5 and AA917702.1. Transcript Variant: This variant (2) uses an alternate in-frame exon in the 5' coding region, compared to variant 1, and encodes isoform F. Isoforms A and F are the same length but differ in the region of amino acids 214-238. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660809.68535.1, U58130.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTCA TCATCATTGG CCTAAGTGTG 
GTAGTAACGA CACTCACAGG TATTTCTATG TCTGCTATTT GCACGAATGG AGTAGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171761.1
CDS	217..3516
Translation	MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_001184832.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184832.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGAGTCA TCATCATTGG CCTAAGTGTG 
GTAGTAACGA CACTCACAGG TATTTCTATG TCTGCTATTT GCACGAATGG AGTAGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005254606.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254663.1 Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

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CloneID	OHu48511
Clone ID Related Accession (Same CDS sequence)	XM_005254606.1 , XM_005254606.2
Accession Version	XM_005254606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGTGTGA TTATCATCGG CTTAGCCGTG 
ACAGTGACTG GTATCACTGG TTTATCCACC TCTGCTATTG CCACAAATGG ATGTGTCAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254663.1
CDS	217..3516
Translation	MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTGITGLSTSAIATNGCVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005254606.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254606.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGTGTGA TTATCATCGG CTTAGCCGTG 
ACAGTGACTG GTATCACTGG TTTATCCACC TCTGCTATTG CCACAAATGG ATGTGTCAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005254606.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254663.1 Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

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CloneID	OHu48511
Clone ID Related Accession (Same CDS sequence)	XM_005254606.1 , XM_005254606.2
Accession Version	XM_005254606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGTGTGA TTATCATCGG CTTAGCCGTG 
ACAGTGACTG GTATCACTGG TTTATCCACC TCTGCTATTG CCACAAATGG ATGTGTCAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254663.1
CDS	216..3515
Translation	MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTGITGLSTSAIATNGCVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005254606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254606.2

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCAG TGCCCAGTAA TACCAATCGC 
TTTCAAGTTA GTGTCATAAA TGAGAACCAT GAGAGCAGTG CAGCTGCAGA TGACAATACT 
GACCCACCAC ATTATGAAGA AACCTCTTTT GGGGATGAAG CTCAGAAAAG ACTCAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAT GACAATTTCC TCCAAAGTGG AGAAACTGCT 
AAAACAGATG CCAGTTTTCA CGCTTATGAT TCTCACACAA ACACATACTA TCTACAAACT 
TTTGGCCACA ACACCATGGA TGCCGTTCCC AAGATAGAGT ACTATCGTAA CACCGGCAGC 
ATCAGTGGGC CCAAGGTCAA CCGACCCAGC CTGCTTGAGA TTCACGAGCA ACTCGCAAAG 
AATGTGGCAG TCACCCCAAG TTCAGCTGAC AGAGTTGCTA ACGGTGATGG GATACCTGGA 
GATGAACAAG CTGAAAATAA GGAAGATGAT CAAGCTGGTG TTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTAAGATG CATGCTGAAC ATCTGGGGAG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATTGGT CTTGGTGTGA TTATCATCGG CTTAGCCGTG 
ACAGTGACTG GTATCACTGG TTTATCCACC TCTGCTATTG CCACAAATGG ATGTGTCAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGTTTAGGGC CCGAGTTCGG TGGGTCAATA 
GGCCTGATCT TTGCTTTTGC TAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTAG TAGATCTTCT TAAGGAGAGT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATTA TAGGCTCCAT CACAGTGGTG ATTCTTCTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAGG CAAAGGCCCA AGTCATTCTT CTGGTCATTC TTCTAATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA AGTCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAACTTT GGGCCACGCT TCACAAAGGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCTACTGGGA TTCTTGCTGG TGCCAATATC 
TCAGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAGAG GAACCATGCT GGCCATTTTC 
ATCACCACTG TTGCCTACTT AGGGGTTGCA ATTTGTGTAG GGGCCTGTGT GGTCCGAGAT 
GCCACCGGGA ACATGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCA 
TGTGGGTTGG GCTATGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA CGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTATCA GGGTTCGGCC CCCTCATCAC TGCGGGAATC 
TTTTCTGCAA CACTCTCCTC CGCCCTGGCC TCCCTTGTCA GCGCACCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCC CTGCAGTTTT TTGCAAAGGG ATATGGGAAA 
AACAATGAAC CCCTGAGAGG ATATATTCTC ACTTTTCTTA TAGCCATGGC ATTTATTCTT 
ATTGCGGAAC TGAACACCAT TGCTCCCATC ATCTCCAACT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT CCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCG 
TATGGAATTT ACAACATGTG GGTATCTCTT TTTGGAGCTG TTTTGTGCTG TGCAGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTATGTCA TTGAATTCTT CCTTTACGTC 
TATGTGACTT GTAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACACAGGC TCTTTCCTAC 
GTGAGTGCTT TAGACAATGC TCTGGAATTA ACCACAGTGG AAGACCACGT AAAAAACTTC 
AGGCCCCAGT GCATTGTCTT AACAGGGGGA CCCATGACAA GACCTGCTCT CCTGGACATA 
ACTCACGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCG 
CGCAAACTGT GTGTTAAGGA GATGAACAGT GGCATGGCGA AAAAACAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCTTT TTATGCTGCA GTGGCGGCAG ACTGTTTCAG GGATGGTGTC 
CGAAGTCTTC TTCAGGCCTC AGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAGAAAA ACTGGAGGAA AGCTCCCTTG ACAGAGATTG AGAACTACGT GGGAATCATA 
CATGATGCAT TTGATTTTGA GATTGGCGTG GTTATAGTCA GAATCAGCCA AGGATTTGAC 
ATCTCTCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAGAT TAGAACAGGA GAGACTAGCA 
TTGGAAGCGA CTATCAAAGA TAATGAGTGT GAAGAGGAAA GTGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGTT GAACATTACT AAGACAACGC CTAAAAAAGA TGGCAGCATT 
AACACAAGCC AGTCGATGCA TGTGGGAGAG TTCAACCAGA AACTGGTGGA AGCCAGCACT 
CAATTTAAAA AGAAACAAGA AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAACAC TTCTTATCCC CTATATCTTA ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTGGGAGG GAAGATCAAC CGCATTGAAG AAGAAAAAAT TGTAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATATCATCGG TGACATCAAC 
ATTAGGCCAA ACAAAGAGAG CTGGAAAGTC TTTGAAGAGA TGATTGAACC ATATCGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AAAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA AGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAT 
GAACTCTTAC AGGAGCACTC CAGAGCTGCT AATCTCATTG TCCTGAGCCT TCCCGTGGCA 
AGAAAGGGAT CCATATCGGA TTTGTTGTAT ATGGCTTGGT TGGAAATCCT CACAAAGAAC 
CTCCCACCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCTTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel SLC12A1 gene mutation associated with hyperparathyroidism, hypercalcemia, nephrogenic diabetes insipidus, and nephrocalcinosis in four patients.
Bone97121-125(2017 04)
Wongsaengsak S,Vidmar AP,Addala A,Kamil ES,Sequeira P,Fass B,Pitukcheewanont P

A novel variant in the SLC12A1 gene in two families with antenatal Bartter syndrome.
Acta paediatrica (Oslo, Norway : 1992)106(1)161-167(2017 Jan)
Breinbjerg A,Siggaard Rittig C,Gregersen N,Rittig S,Hvarregaard Christensen J

Antenatal Bartter syndrome presenting as hyperparathyroidism with hypercalcemia and hypercalciuria: a case report and review.
Journal of pediatric endocrinology & metabolism : JPEM28(7-8)943-6(2015 Jul)
Gross I,Siedner-Weintraub Y,Simckes A,Gillis D

Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and functional significance.
American journal of physiology. Renal physiology295(4)F859-66(2008 Oct)
Castrop H,Schnermann J

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SLC12A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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