slc12a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a1
Entrez Gene ID	100490198
Full Name	solute carrier family 12 member 1
Synonyms	NKCC-2,NKCC2,XNKCC2
General protein information	Preferred Names solute carrier family 12 member 1 Names solute carrier family 12 member 1 Na-K-2Cl cotransporter solute carrier family 12 (sodium/potassium/chloride transporter), member 1 solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012959479.2	XP_012814933.1	solute carrier family 12 member 1 isoform X2
XM_012959479.3	XP_012814933.2	solute carrier family 12 member 1 isoform X2
XM_002936567.5	XP_002936613.2	solute carrier family 12 member 1 isoform X1
XM_012959480.2	XP_012814934.1	solute carrier family 12 member 1 isoform X3
XM_002936567.4	XP_002936613.2	solute carrier family 12 member 1 isoform X1
XM_004912598.3	XP_004912655.1	solute carrier family 12 member 1 isoform X1
XM_012959480.3	XP_012814934.1	solute carrier family 12 member 1 isoform X3

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Gene Ontology

The following slc12a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa55108	XM_002936567.5 Latest version!	Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa55109	XM_012959479.3 Latest version!	Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa55110	XM_012959480.3 Latest version!	Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa07208	XM_002936567.4 Latest version!	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa07208	XM_004912598.3 Latest version!	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa07209	XM_012959479.2 Latest version!	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa07210	XM_012959480.2 Latest version!	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a1 ( XM_002936567.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936613.2 Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55108
Clone ID Related Accession (Same CDS sequence)	XM_002936567.5
Accession Version	XM_002936567.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936567.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTTGTTA TCATCTGCCT GAGTGTGGTA GTTACTTCAT TAACAGGTGT CTCTATGTCT 
GCTATATGCA CCAATGGTGT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCACGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTCA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936613.2
CDS	246..3506
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVVIICLSVVVTSLTGVSMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_002936567.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936567.5

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTTGTTA TCATCTGCCT GAGTGTGGTA GTTACTTCAT TAACAGGTGT CTCTATGTCT 
GCTATATGCA CCAATGGTGT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCACGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTCA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a1 ( XM_012959479.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814933.2 Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa55109
Clone ID Related Accession (Same CDS sequence)	XM_012959479.3
Accession Version	XM_012959479.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTG 
GCAGTTGTCA TAATTCTTCT TGCTACCATG GTTACCTCAA TTACTGGGTT ATCTACTTCT 
GCAATAGCTA CCAACGGCTT TGTCCGTGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCACGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTCA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814933.2
CDS	246..3506
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLAVVIILLATMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_012959479.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959479.3

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTG 
GCAGTTGTCA TAATTCTTCT TGCTACCATG GTTACCTCAA TTACTGGGTT ATCTACTTCT 
GCAATAGCTA CCAACGGCTT TGTCCGTGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCACGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTCA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a1 ( XM_012959480.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814934.1 Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X3, mRNA.

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CloneID	OXa55110
Clone ID Related Accession (Same CDS sequence)	XM_012959480.3
Accession Version	XM_012959480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTCATTA TCATCGGGCT AGCTGTGACA GTTACTGCTA TAACTGGCCT GTCCACCTCT 
GCAATTGCCA CAAATGGATT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCACGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTCA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814934.1
CDS	246..3506
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVIIIGLAVTVTAITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_012959480.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 1 (slc12a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959480.3

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTCATTA TCATCGGGCT AGCTGTGACA GTTACTGCTA TAACTGGCCT GTCCACCTCT 
GCAATTGCCA CAAATGGATT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCACGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTCA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a1 ( XM_002936567.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936613.2 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07208
Clone ID Related Accession (Same CDS sequence)	XM_002936567.4 , XM_004912598.3
Accession Version	XM_002936567.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936567.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTTGTTA TCATCTGCCT GAGTGTGGTA GTTACTTCAT TAACAGGTGT CTCTATGTCT 
GCTATATGCA CCAATGGTGT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936613.2
CDS	228..3488
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVVIICLSVVVTSLTGVSMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_002936567.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936567.4

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTTGTTA TCATCTGCCT GAGTGTGGTA GTTACTTCAT TAACAGGTGT CTCTATGTCT 
GCTATATGCA CCAATGGTGT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a1 ( XM_004912598.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912655.1 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X3, mRNA.

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CloneID	OXa07208
Clone ID Related Accession (Same CDS sequence)	XM_002936567.4 , XM_004912598.3
Accession Version	XM_004912598.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTTGTTA TCATCTGCCT GAGTGTGGTA GTTACTTCAT TAACAGGTGT CTCTATGTCT 
GCTATATGCA CCAATGGTGT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912655.1
CDS	204..3464
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVVIICLSVVVTSLTGVSMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_004912598.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912598.3

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTTGTTA TCATCTGCCT GAGTGTGGTA GTTACTTCAT TAACAGGTGT CTCTATGTCT 
GCTATATGCA CCAATGGTGT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a1 ( XM_012959479.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814933.1 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07209
Clone ID Related Accession (Same CDS sequence)	XM_012959479.2
Accession Version	XM_012959479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTG 
GCAGTTATCA TAATTCTTCT TGCTACCATG GTTACCTCAA TTACTGGGTT ATCTACTTCT 
GCAATAGCTA CCAACGGCTT TGTCCGTGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814933.1
CDS	228..3488
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLAVIIILLATMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_012959479.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959479.2

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTG 
GCAGTTATCA TAATTCTTCT TGCTACCATG GTTACCTCAA TTACTGGGTT ATCTACTTCT 
GCAATAGCTA CCAACGGCTT TGTCCGTGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a1 ( XM_012959480.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814934.1 Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa07210
Clone ID Related Accession (Same CDS sequence)	XM_012959480.2
Accession Version	XM_012959480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTCATTA TCATCGGGCT AGCTGTGACA GTTACTGCTA TAACTGGCCT GTCCACCTCT 
GCAATTGCCA CAAATGGATT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814934.1
CDS	228..3488
Translation	MNNSPNTFLDTAPISPSRFQVNVVGEGYDNPACEDKDPPDYEETSFADDNRMSYRPGNREIFNNVLHNGDTIKTEGSFHPYDTHGHAYYMKTFGHNTVDAVPKIDYYRNTGSISGVKICRPSLADIHEQLSKNVVEVPGSVTNGEGIPGDEEANKEEGEKGGFVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVIIIGLAVTVTAITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFSETVVEILKDTNTIMVDEVNDIRIIGTITVILLLGISVAGMEWEAKAQVVLLVILLIAIVNFFIGTVIPTTKEKRAQGFFNYQATIFAENFGPDFRNGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMWAILITTIAYLGVAICAAASVVRDATGNLNDTITSAFKCNGSAACGLGYDFSICETQTCNYGLMNNFQVMSMVSGFGPLIIAGIFSATLSSALASLVSAPKVFQALCKDNIYKGLHFFAKGHGKNNEPIRGYVLTFVIALAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFKYYNMWVSLFGAMLCCGVMFVINWWAALITYAIELFLYIYVTYKKPDVNWGSSTQALQFKNALDSALGLNCIEDHVKNFRPQCIVLTGVPMTRPALLDITHSFTKNSGLCICCEVFSGPRKQAVKEMNSGMIKKQTWLTNNKRKAFYAAVAADNFRDGVRTLLQASGLGRLKPNTLVMGFKKDWRQASPVDLANYVGILHDAFDFEHGVVIVRISQGFDISQVLQVKEEFDRLEQERLALEQTIKENEFKEGNHGLRSFFNKTAKLNINRHSSTKDVSTPPPMHVGEYNQRLVEASEQFKKNQGKGMIDVWWLFDDGGLTLLIPHILTLRKKWKDCKLRIFIGGKVNRLEDEKLMMASLLSKFRIKFADIYIVGDINTKPSKESWKFFEEMIEPYCLHENSTDVSTAEKIRRENPWKITDSELEMFKEKSYRQVRLNELLQEHSRSANLIVLSLPVARKGSVSDHLYMAWVEILSKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_012959480.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (slc12a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959480.2

ORF Insert Sequence:

ATGAACAATT CTCCAAACAC ATTTCTCGAT ACAGCTCCCA TTAGCCCATC ACGTTTCCAA 
GTAAATGTAG TTGGTGAAGG GTATGACAAT CCTGCTTGCG AAGACAAAGA CCCACCAGAT 
TATGAGGAAA CATCCTTTGC TGATGACAAT AGAATGAGTT ACAGGCCAGG TAATAGGGAA 
ATATTTAACA ATGTTCTACA CAATGGAGAC ACGATAAAGA CAGAAGGAAG TTTTCATCCT 
TATGACACCC ATGGTCATGC TTATTATATG AAAACATTTG GACACAATAC AGTGGATGCT 
GTTCCAAAGA TTGATTATTA CAGAAACACT GGAAGCATCT CAGGAGTCAA GATATGCAGA 
CCAAGTCTGG CAGATATCCA TGAGCAGCTG TCAAAGAATG TGGTTGAGGT TCCTGGATCC 
GTCACCAATG GAGAAGGGAT TCCAGGTGAT GAAGAAGCAA ACAAGGAAGA AGGCGAAAAG 
GGTGGATTTG TGAAATTTGG ATGGATAAAG GGTGTACTGG TCAGATGCAT GCTAAATATA 
TGGGGTGTGA TGCTGTTCAT TCGGCTCTCC TGGATTGTAG GACAAGCTGG GATCGGTCTT 
GGTGTCATTA TCATCGGGCT AGCTGTGACA GTTACTGCTA TAACTGGCCT GTCCACCTCT 
GCAATTGCCA CAAATGGATT TGTCAGAGGA GGTGGAGCGT ACTATCTCAT ATCGAGAAGT 
TTGGGGCCTG AGTTTGGTGG ATCCATAGGA CTGATTTTTG CTTTTGCTAA TGCCGTCGCT 
GTTGCTATGT ATGTGGTTGG ATTCTCTGAA ACTGTTGTGG AAATTCTTAA GGACACCAAT 
ACGATCATGG TTGATGAAGT CAATGACATA AGAATAATAG GTACCATAAC AGTCATCCTC 
CTTCTTGGCA TATCTGTGGC GGGTATGGAG TGGGAAGCTA AGGCTCAGGT TGTGCTATTA 
GTTATCCTAC TCATTGCAAT TGTAAATTTC TTCATTGGGA CTGTTATTCC AACCACCAAG 
GAGAAGAGGG CGCAGGGCTT CTTTAATTAC CAGGCGACAA TATTTGCTGA AAACTTCGGA 
CCAGATTTCA GGAACGGAGA AGGATTTTTT TCAGTATTTG CTATTTTCTT CCCTGCTGCT 
ACTGGAATTC TCGCTGGGGC AAACATTTCT GGTGATTTAA AGGATCCTCA AGGCGCTATC 
CCAAAAGGAA CAATGTGGGC TATTTTGATC ACAACAATTG CCTATCTGGG AGTTGCTATT 
TGTGCAGCGG CTTCTGTAGT GCGGGATGCC ACTGGGAATC TGAATGACAC AATCACCTCA 
GCATTTAAGT GCAATGGTTC TGCGGCCTGT GGCCTTGGCT ATGATTTTTC CATTTGTGAA 
ACACAAACAT GTAACTATGG CTTGATGAAT AACTTTCAAG TTATGAGTAT GGTGTCTGGG 
TTTGGACCTT TGATTATTGC TGGCATCTTC TCTGCTACAC TTTCTTCAGC TTTGGCATCA 
CTTGTCAGTG CACCCAAAGT ATTTCAGGCT CTCTGCAAGG ACAACATATA CAAAGGACTT 
CATTTCTTTG CCAAAGGACA TGGAAAGAAT AATGAGCCAA TCAGAGGATA CGTCCTAACT 
TTTGTAATTG CGCTTGCATT TATTCTGATT GCTGAACTGA ATGTAATTGC ACCCATCATT 
TCCAACTTCT TCCTGGCTTC TTATGCCTTG ATCAACTTCT CCTGCTTTCA TGCTTCATAT 
GCTAAATCCC CAGGCTGGAG GCCTGCTTTC AAGTATTACA ACATGTGGGT GTCCCTGTTT 
GGAGCCATGC TGTGCTGTGG TGTGATGTTT GTCATCAACT GGTGGGCTGC TCTCATCACT 
TATGCCATTG AGCTTTTCTT GTACATCTAT GTGACATACA AGAAACCAGA TGTAAATTGG 
GGATCATCAA CCCAGGCTCT TCAATTCAAA AATGCCTTGG ACAGTGCATT AGGTTTAAAC 
TGCATTGAAG ATCATGTGAA AAACTTCAGA CCTCAATGCA TCGTTTTAAC TGGAGTGCCA 
ATGACAAGAC CAGCTCTTCT TGATATAACA CACTCATTTA CAAAGAACTC TGGGCTGTGC 
ATCTGTTGTG AAGTGTTTTC GGGGCCGCGC AAGCAAGCTG TAAAAGAAAT GAACAGTGGG 
ATGATCAAGA AGCAGACATG GTTAACAAAT AACAAAAGAA AAGCATTTTA TGCAGCAGTT 
GCTGCTGACA ATTTTAGAGA CGGTGTAAGA ACTCTCCTTC AGGCTTCTGG ATTAGGAAGA 
CTGAAACCAA ATACCCTGGT GATGGGATTT AAGAAGGACT GGAGGCAAGC AAGCCCTGTG 
GATCTTGCCA ACTATGTGGG CATTTTACAT GATGCTTTTG ATTTTGAACA TGGAGTGGTT 
ATTGTAAGAA TCAGCCAAGG CTTTGATATA TCTCAGGTTC TTCAGGTTAA AGAGGAATTT 
GACAGACTGG AGCAAGAGAG ACTGGCATTA GAACAGACTA TCAAAGAGAA TGAATTTAAA 
GAAGGGAATC ATGGACTCCG TAGTTTCTTC AATAAAACCG CCAAGCTGAA TATCAACAGG 
CATTCTTCAA CTAAAGATGT CAGCACACCT CCACCCATGC ATGTGGGCGA GTACAATCAG 
AGGCTGGTTG AAGCTAGTGA GCAGTTTAAA AAGAATCAAG GAAAGGGTAT GATTGATGTC 
TGGTGGCTGT TTGATGACGG AGGACTGACT CTTCTTATTC CACACATTCT GACCCTCAGA 
AAAAAATGGA AAGACTGCAA ATTAAGAATT TTTATAGGAG GAAAAGTGAA TCGTCTGGAA 
GATGAAAAAC TCATGATGGC ATCACTTTTA AGCAAATTTC GAATTAAATT TGCTGATATT 
TACATTGTTG GAGACATTAA CACAAAGCCA AGCAAAGAAA GCTGGAAATT CTTTGAAGAA 
ATGATAGAGC CATATTGTCT CCATGAGAAC TCTACAGATG TGTCAACTGC AGAAAAAATA 
AGGAGAGAAA ATCCATGGAA AATAACAGAC TCTGAACTGG AGATGTTTAA GGAAAAGAGT 
TACCGCCAAG TCAGACTGAA TGAGTTATTA CAAGAACACT CCAGATCTGC CAATCTCATT 
GTCTTAAGTC TTCCTGTTGC AAGAAAAGGA TCCGTCTCAG ATCACCTTTA TATGGCATGG 
GTAGAAATTC TTTCAAAGAA CCTTCCACCT GTCTTGCTGG TTCGAGGAAA TCACAAGAAT 
GTACTAACAT TTTATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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slc12a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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