lig-1 cDNA ORF clone, Caenorhabditis elegans

The following lig-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lig-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa344980 NM_171538.4
Latest version!
Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OCa344979 NM_171537.4
Latest version!
Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OCa344980 NM_171538.3 Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCa344979 NM_171537.3 Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa344980
    Clone ID Related Accession (Same CDS sequence) NM_171538.4 , NM_171538.3
    Accession Version NM_171538.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DNA ligase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_171538.3. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GCGAATGCCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_741626.2
    CDS1..633
    Translation

    Target ORF information:

    RefSeq Version NM_171538.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_171538.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GCGAATGCCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa344980
    Clone ID Related Accession (Same CDS sequence) NM_171538.4 , NM_171538.3
    Accession Version NM_171538.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DNA ligase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_171538.2. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GCGAATGCCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_741626.2
    CDS1..633
    Translation

    Target ORF information:

    RefSeq Version NM_171538.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_171538.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GCGAATGCCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa344979
    Clone ID Related Accession (Same CDS sequence) NM_171537.3 , NM_171537.4
    Accession Version NM_171537.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DNA ligase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_171537.2. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GGTTCGTGGT GACAAACTCG ATTCTCTGAA AGATGCAAAT
    GTTAAGCGAG TAAAGACCGC CTATTGCGAA TGCCCTAACT ACAATCGTCT GATCGAAGTT
    GCACTGACTG AAGGTGTGGA AGCTCTTGTC GAAAAATGTA AACTTTCACC CGGAATTCCA
    CTGAAACCTA TGCTGGCTCA CCCTACAAAG GGAATTGATG AAATTATGCG ACGATTCAGA
    AATCAGACAA TGACCTGTGA ATGGAAATAT GACGGAGAAC GCGGCCAGAT TCATAAACGA
    GAAGACGGGC AAATTTTCAT CTACAGTCGA AACCAAGAGA ACAACACTAC CAAGTACCCC
    GATATTATCG AAAAAATTTC ATCCTGCATC GGGGATGGGG TAACTTCATT TATTGTCGAT
    GCTGAAGTTG TAGCTATCGA TGAGGCTGGC TTAATTCTTC CGTTCCAAGT TTTGTCGACT
    CGTAAAAGGA AAAATGCAAC TGATGATAAC GGAGTCAAGG TTGTAGTTTT TCTTTTCGAT
    CTTCTCTACT TCAATGGGGA GCCTCTCGTG AGAAAACCAC TCCGAAAAAG AAGAGAACTT
    CTTCGAACAA ACTTCAAAAA GATTGATGGA AGCTTCTATT TTGCAACTTC CGTAGATACA
    AATGATACTG ATGAAATCAA CTCTTTCTTC GATGAAGCCG TTCAAAATAA ATGCGAAGGA
    CTCATGATCA AAACTCTCGA CACTGAAGCT ACTTATGAGA TTTCAAGACG TTCCCACAGC
    TGGTTGAAAA TGAAGAAAGA TTACGTTGAT GGAGTAGGAG ATACGTTGGA TCTTGTCGTC
    ATGGGTGCTT ATTCTGGAGT TGGAAAGAGA ACTGGAGTGT ATGGAGGATA TCTTCTCGGA
    TGTTACAATC CTACTACAGA AGAGTACGAA AGTGTATGCA AGATTGGAAC TGGTTTCACT
    GATGAAGATT TGGCCGAGCA ATACAAGATC CTACAAGACA AGAAAATTGA CAAATCTCCT
    TCTTATTATC AATTTGATCA CACTCTTAAA CCAGATGACA CTTTTTCTCC GTATCTTGTT
    TTCGAAGTCA AATGTGCCGA TATTACTATA TCTCCGCGTC ACAAAGCTGC AAGTGGATTG
    ACGGATGATG GAAAAGGAAT CTCTTTGAGA TTTCCTCGTT TCCTGCGTAT TCGGGACGAT
    AAAAATTCCG ACGATGCAAC ATCTTCGGAG CAAGTTCTTG AAATGTACAA AAACCAAGAA
    GCTTTTGCCA ACCAGAAGAT CGAAAAGGCG GATGCAGTTG ATGAGGATGA CGAATTTGAA
    GAAAAAGAAG ATGAAGAGGA GGAGTTGAAC ATGACCAATG TATCTGAAGG AAGTTCTAAA
    GAAAATCCAG TGAAAGAGGA GATCAAGAAA GAGACTCCTA AATCAGTATC CCCGAAAAAA
    TTCGAAAAGA AACCGCCTGT AAAATCTTCA CCAGTTAACA AATCGCCCGT GAAAAGTTCA
    CCAATAAAGA AAGAAGCAGA GAAAAAGAAA GGACCAGTTG CTAGCATCTT TTCATCATCG
    ACAAAGAAGA ATGAAAAAGA CGTTAAAGTG GAATCTCCAT CTCCGATCAG AAAGAAGAAG
    CTGCCTTCTG ATTCTGACGA GTCTGATGAA GAAACCTCAA CTAATAAGAA ACAGCCAGCA
    AAGAAGAGGT CTCGCGTTGC AATTGATTCC GATTCCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_741625.2
    CDS1..2322
    Translation

    Target ORF information:

    RefSeq Version NM_171537.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_171537.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GGTTCGTGGT GACAAACTCG ATTCTCTGAA AGATGCAAAT
    GTTAAGCGAG TAAAGACCGC CTATTGCGAA TGCCCTAACT ACAATCGTCT GATCGAAGTT
    GCACTGACTG AAGGTGTGGA AGCTCTTGTC GAAAAATGTA AACTTTCACC CGGAATTCCA
    CTGAAACCTA TGCTGGCTCA CCCTACAAAG GGAATTGATG AAATTATGCG ACGATTCAGA
    AATCAGACAA TGACCTGTGA ATGGAAATAT GACGGAGAAC GCGGCCAGAT TCATAAACGA
    GAAGACGGGC AAATTTTCAT CTACAGTCGA AACCAAGAGA ACAACACTAC CAAGTACCCC
    GATATTATCG AAAAAATTTC ATCCTGCATC GGGGATGGGG TAACTTCATT TATTGTCGAT
    GCTGAAGTTG TAGCTATCGA TGAGGCTGGC TTAATTCTTC CGTTCCAAGT TTTGTCGACT
    CGTAAAAGGA AAAATGCAAC TGATGATAAC GGAGTCAAGG TTGTAGTTTT TCTTTTCGAT
    CTTCTCTACT TCAATGGGGA GCCTCTCGTG AGAAAACCAC TCCGAAAAAG AAGAGAACTT
    CTTCGAACAA ACTTCAAAAA GATTGATGGA AGCTTCTATT TTGCAACTTC CGTAGATACA
    AATGATACTG ATGAAATCAA CTCTTTCTTC GATGAAGCCG TTCAAAATAA ATGCGAAGGA
    CTCATGATCA AAACTCTCGA CACTGAAGCT ACTTATGAGA TTTCAAGACG TTCCCACAGC
    TGGTTGAAAA TGAAGAAAGA TTACGTTGAT GGAGTAGGAG ATACGTTGGA TCTTGTCGTC
    ATGGGTGCTT ATTCTGGAGT TGGAAAGAGA ACTGGAGTGT ATGGAGGATA TCTTCTCGGA
    TGTTACAATC CTACTACAGA AGAGTACGAA AGTGTATGCA AGATTGGAAC TGGTTTCACT
    GATGAAGATT TGGCCGAGCA ATACAAGATC CTACAAGACA AGAAAATTGA CAAATCTCCT
    TCTTATTATC AATTTGATCA CACTCTTAAA CCAGATGACA CTTTTTCTCC GTATCTTGTT
    TTCGAAGTCA AATGTGCCGA TATTACTATA TCTCCGCGTC ACAAAGCTGC AAGTGGATTG
    ACGGATGATG GAAAAGGAAT CTCTTTGAGA TTTCCTCGTT TCCTGCGTAT TCGGGACGAT
    AAAAATTCCG ACGATGCAAC ATCTTCGGAG CAAGTTCTTG AAATGTACAA AAACCAAGAA
    GCTTTTGCCA ACCAGAAGAT CGAAAAGGCG GATGCAGTTG ATGAGGATGA CGAATTTGAA
    GAAAAAGAAG ATGAAGAGGA GGAGTTGAAC ATGACCAATG TATCTGAAGG AAGTTCTAAA
    GAAAATCCAG TGAAAGAGGA GATCAAGAAA GAGACTCCTA AATCAGTATC CCCGAAAAAA
    TTCGAAAAGA AACCGCCTGT AAAATCTTCA CCAGTTAACA AATCGCCCGT GAAAAGTTCA
    CCAATAAAGA AAGAAGCAGA GAAAAAGAAA GGACCAGTTG CTAGCATCTT TTCATCATCG
    ACAAAGAAGA ATGAAAAAGA CGTTAAAGTG GAATCTCCAT CTCCGATCAG AAAGAAGAAG
    CTGCCTTCTG ATTCTGACGA GTCTGATGAA GAAACCTCAA CTAATAAGAA ACAGCCAGCA
    AAGAAGAGGT CTCGCGTTGC AATTGATTCC GATTCCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa344979
    Clone ID Related Accession (Same CDS sequence) NM_171537.3 , NM_171537.4
    Accession Version NM_171537.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DNA ligase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_171537.3. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GGTTCGTGGT GACAAACTCG ATTCTCTGAA AGATGCAAAT
    GTTAAGCGAG TAAAGACCGC CTATTGCGAA TGCCCTAACT ACAATCGTCT GATCGAAGTT
    GCACTGACTG AAGGTGTGGA AGCTCTTGTC GAAAAATGTA AACTTTCACC CGGAATTCCA
    CTGAAACCTA TGCTGGCTCA CCCTACAAAG GGAATTGATG AAATTATGCG ACGATTCAGA
    AATCAGACAA TGACCTGTGA ATGGAAATAT GACGGAGAAC GCGGCCAGAT TCATAAACGA
    GAAGACGGGC AAATTTTCAT CTACAGTCGA AACCAAGAGA ACAACACTAC CAAGTACCCC
    GATATTATCG AAAAAATTTC ATCCTGCATC GGGGATGGGG TAACTTCATT TATTGTCGAT
    GCTGAAGTTG TAGCTATCGA TGAGGCTGGC TTAATTCTTC CGTTCCAAGT TTTGTCGACT
    CGTAAAAGGA AAAATGCAAC TGATGATAAC GGAGTCAAGG TTGTAGTTTT TCTTTTCGAT
    CTTCTCTACT TCAATGGGGA GCCTCTCGTG AGAAAACCAC TCCGAAAAAG AAGAGAACTT
    CTTCGAACAA ACTTCAAAAA GATTGATGGA AGCTTCTATT TTGCAACTTC CGTAGATACA
    AATGATACTG ATGAAATCAA CTCTTTCTTC GATGAAGCCG TTCAAAATAA ATGCGAAGGA
    CTCATGATCA AAACTCTCGA CACTGAAGCT ACTTATGAGA TTTCAAGACG TTCCCACAGC
    TGGTTGAAAA TGAAGAAAGA TTACGTTGAT GGAGTAGGAG ATACGTTGGA TCTTGTCGTC
    ATGGGTGCTT ATTCTGGAGT TGGAAAGAGA ACTGGAGTGT ATGGAGGATA TCTTCTCGGA
    TGTTACAATC CTACTACAGA AGAGTACGAA AGTGTATGCA AGATTGGAAC TGGTTTCACT
    GATGAAGATT TGGCCGAGCA ATACAAGATC CTACAAGACA AGAAAATTGA CAAATCTCCT
    TCTTATTATC AATTTGATCA CACTCTTAAA CCAGATGACA CTTTTTCTCC GTATCTTGTT
    TTCGAAGTCA AATGTGCCGA TATTACTATA TCTCCGCGTC ACAAAGCTGC AAGTGGATTG
    ACGGATGATG GAAAAGGAAT CTCTTTGAGA TTTCCTCGTT TCCTGCGTAT TCGGGACGAT
    AAAAATTCCG ACGATGCAAC ATCTTCGGAG CAAGTTCTTG AAATGTACAA AAACCAAGAA
    GCTTTTGCCA ACCAGAAGAT CGAAAAGGCG GATGCAGTTG ATGAGGATGA CGAATTTGAA
    GAAAAAGAAG ATGAAGAGGA GGAGTTGAAC ATGACCAATG TATCTGAAGG AAGTTCTAAA
    GAAAATCCAG TGAAAGAGGA GATCAAGAAA GAGACTCCTA AATCAGTATC CCCGAAAAAA
    TTCGAAAAGA AACCGCCTGT AAAATCTTCA CCAGTTAACA AATCGCCCGT GAAAAGTTCA
    CCAATAAAGA AAGAAGCAGA GAAAAAGAAA GGACCAGTTG CTAGCATCTT TTCATCATCG
    ACAAAGAAGA ATGAAAAAGA CGTTAAAGTG GAATCTCCAT CTCCGATCAG AAAGAAGAAG
    CTGCCTTCTG ATTCTGACGA GTCTGATGAA GAAACCTCAA CTAATAAGAA ACAGCCAGCA
    AAGAAGAGGT CTCGCGTTGC AATTGATTCC GATTCCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_741625.2
    CDS1..2322
    Translation

    Target ORF information:

    RefSeq Version NM_171537.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DNA ligase 1 (lig-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_171537.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTTTTCT CAAAAGCTGG CAAAGCCGCT GTTGAATGGG CAAAGGGTTC AAAAGTCCCT 
    TACAAGGAGT TTGCTCTCAC CCTTGAGAAA ATCGAAGAGT TATCGGGAAA AAAGAAAGTG
    GATGAATTGG CTCAATTTTT CACCAAAGTT CTGGACTTTT CACCCGACGA TTTAACAGCG
    TGCGTGTACA TGTCAGTTAA TCAGCTCGGG CCATCGTATG AAGGGCTCGA GCTTGGAGTT
    GCTGAAAATT CGCTGATCAA GGCTGTTGCT AAAGCGACCG GACGTACAGA GGGAAAAATT
    AAAGAAGATC TTCGCGCGAA AGGAGATCTT GGGACTGTCG CACAACAGTC AAGATCCAAT
    CAGAAAATGC TTGCTGTTCC AAAAGCATTA ACTGTTCCAA CTGTTTTCAA CAAGTTAACC
    GAAATTGCAA AGCTCAGTGG CACTTCAGCT ATGAACAAGA AAGTTGATGC CATTAGTGCA
    CTCCTCATTG CATGTCAAGG AATTGAGGCT CGATTCCTCG TCCGAATGCT CGCCGGCAAA
    ATGAGAATCG GTCTCGGTGA ACAAAGTGTT CTATCAGCAC TTGGACATGC ATTCACTCTT
    TCGAAAATAA CTGATCAAAA GGTTCGTGGT GACAAACTCG ATTCTCTGAA AGATGCAAAT
    GTTAAGCGAG TAAAGACCGC CTATTGCGAA TGCCCTAACT ACAATCGTCT GATCGAAGTT
    GCACTGACTG AAGGTGTGGA AGCTCTTGTC GAAAAATGTA AACTTTCACC CGGAATTCCA
    CTGAAACCTA TGCTGGCTCA CCCTACAAAG GGAATTGATG AAATTATGCG ACGATTCAGA
    AATCAGACAA TGACCTGTGA ATGGAAATAT GACGGAGAAC GCGGCCAGAT TCATAAACGA
    GAAGACGGGC AAATTTTCAT CTACAGTCGA AACCAAGAGA ACAACACTAC CAAGTACCCC
    GATATTATCG AAAAAATTTC ATCCTGCATC GGGGATGGGG TAACTTCATT TATTGTCGAT
    GCTGAAGTTG TAGCTATCGA TGAGGCTGGC TTAATTCTTC CGTTCCAAGT TTTGTCGACT
    CGTAAAAGGA AAAATGCAAC TGATGATAAC GGAGTCAAGG TTGTAGTTTT TCTTTTCGAT
    CTTCTCTACT TCAATGGGGA GCCTCTCGTG AGAAAACCAC TCCGAAAAAG AAGAGAACTT
    CTTCGAACAA ACTTCAAAAA GATTGATGGA AGCTTCTATT TTGCAACTTC CGTAGATACA
    AATGATACTG ATGAAATCAA CTCTTTCTTC GATGAAGCCG TTCAAAATAA ATGCGAAGGA
    CTCATGATCA AAACTCTCGA CACTGAAGCT ACTTATGAGA TTTCAAGACG TTCCCACAGC
    TGGTTGAAAA TGAAGAAAGA TTACGTTGAT GGAGTAGGAG ATACGTTGGA TCTTGTCGTC
    ATGGGTGCTT ATTCTGGAGT TGGAAAGAGA ACTGGAGTGT ATGGAGGATA TCTTCTCGGA
    TGTTACAATC CTACTACAGA AGAGTACGAA AGTGTATGCA AGATTGGAAC TGGTTTCACT
    GATGAAGATT TGGCCGAGCA ATACAAGATC CTACAAGACA AGAAAATTGA CAAATCTCCT
    TCTTATTATC AATTTGATCA CACTCTTAAA CCAGATGACA CTTTTTCTCC GTATCTTGTT
    TTCGAAGTCA AATGTGCCGA TATTACTATA TCTCCGCGTC ACAAAGCTGC AAGTGGATTG
    ACGGATGATG GAAAAGGAAT CTCTTTGAGA TTTCCTCGTT TCCTGCGTAT TCGGGACGAT
    AAAAATTCCG ACGATGCAAC ATCTTCGGAG CAAGTTCTTG AAATGTACAA AAACCAAGAA
    GCTTTTGCCA ACCAGAAGAT CGAAAAGGCG GATGCAGTTG ATGAGGATGA CGAATTTGAA
    GAAAAAGAAG ATGAAGAGGA GGAGTTGAAC ATGACCAATG TATCTGAAGG AAGTTCTAAA
    GAAAATCCAG TGAAAGAGGA GATCAAGAAA GAGACTCCTA AATCAGTATC CCCGAAAAAA
    TTCGAAAAGA AACCGCCTGT AAAATCTTCA CCAGTTAACA AATCGCCCGT GAAAAGTTCA
    CCAATAAAGA AAGAAGCAGA GAAAAAGAAA GGACCAGTTG CTAGCATCTT TTCATCATCG
    ACAAAGAAGA ATGAAAAAGA CGTTAAAGTG GAATCTCCAT CTCCGATCAG AAAGAAGAAG
    CTGCCTTCTG ATTCTGACGA GTCTGATGAA GAAACCTCAA CTAATAAGAA ACAGCCAGCA
    AAGAAGAGGT CTCGCGTTGC AATTGATTCC GATTCCGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.