ama-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ama-1
Entrez Gene ID	177190
Full Name	DNA-directed RNA polymerase II subunit RPB1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_068122.7	NP_500523.4	DNA-directed RNA polymerase II subunit RPB1
NM_068122.6	NP_500523.4	DNA-directed RNA polymerase II subunit RPB1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Homo sapiens (human)	POLR2A	NP_000928.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ama-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ama-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa343837	NM_068122.7 Latest version!	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB1 (ama-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa343837	NM_068122.6	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB1 (ama-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ama-1 ( NM_068122.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_500523.4 Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB1 (ama-1), mRNA.

Price & Availability↑

CloneID	OCa343837
Clone ID Related Accession (Same CDS sequence)	NM_068122.7 , NM_068122.6
Accession Version	NM_068122.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase II subunit RPB1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_068122.6.

ATGGCTCTCG TCGGTGTCGA CTTCCAAGCG CCGCTGCGCA TTGTCTCACG CGTTCAGTTT 
GGAATTCTCG GCCCGGAGGA GATTAAACGC ATGTCAGTGG CTCATGTCGA GTTTCCAGAA 
GTCTACGAGA ACGGAAAGCC AAAGTTGGGC GGTCTCATGG ATCCAAGACA AGGAGTCATA 
GATCGTCGTG GAAGATGCAT GACATGCGCT GGAAATTTGA CAGATTGCCC GGGACATTTC 
GGTCATTTGG AGCTCGCCAA ACCCGTATTT CACATTGGAT TCCTCACAAA AACGCTGAAA 
ATACTGCGTT GCGTATGCTT CTACTGCGGA CGGCTTCTCA TCGACAAATC CGCTCCTCGA 
GTGCTGGAAA TTCTGAAAAA GACTGGCACA AACTCGAAAA AGCGGCTCAC AATGATCTAC 
GATCTGTGCA AAGCCAAGTC GGTGTGCGAA GGAGCAGCGG AGAAGGAAGA AGGAATGCCA 
GATGACCCGG ATGATCCGAT GAATGATGGA AAGAAGGTCG CAGGTGGATG CGGTCGCTAT 
CAACCATCAT ACCGCCGTGT CGGCATCGAC ATCAACGCAG AATGGAAGAA GAACGTGAAT 
GAGGACACGC AGGAGCGAAA AATCATGCTG ACCGCCGAAC GAGTACTCGA AGTCTTCCAA 
CAGATCACAG ACGAAGATAT TCTCGTAATA GGAATGGATC CACAATTCGC TCGGCCTGAA 
TGGATGATTT GTACCGTACT TCCAGTCCCA CCACTTGCAG TTCGTCCAGC AGTCGTCACA 
TTTGGATCGG CAAAGAATCA GGACGATTTG ACCCACAAAC TTTCGGATAT TATCAAAACC 
AATCAGCAGT TGCAGAGAAA TGAGGCTAAC GGAGCCGCAG CCCATGTGCT CACCGATGAC 
GTCAGGCTTT TGCAGTTCCA CGTGGCAACT CTTGTGGACA ATTGTATTCC GGGCCTGCCG 
ACGGCTACAC AGAAGGGTGG ACGACCGCTG AAATCGATCA AGCAGCGACT GAAAGGAAAA 
GAAGGACGCA TTCGTGGAAA TTTGATGGGA AAACGTGTGG ATTTCTCTGC GCGTACCGTC 
ATCACAGCCG ATCCGAACCT GCCGATTGAT ACGGTCGGTG TGCCGAGAAC TATTGCGCAG 
AATCTTACGT TCCCGGAAAT TGTGACACCT TTTAATGTGG ATAAGCTGCA GGAGTTGGTC 
AATCGTGGTG ATACACAGTA TCCTGGTGCC AAGTATATTA TTAGAGAAAA CGGAGCTCGA 
GTGGATCTTC GCTACCATCC GCGTGCCGCC GATCTTCACC TCCAACCGGG ATACCGTGTC 
GAACGTCACA TGAAAGATGG CGATATAATC GTCTTTAATC GTCAACCGAC TCTCCACAAA 
ATGTCAATGA TGGGACATCG TGTCAAAATC CTCCCATGGA GTACATTCCG AATGAATTTG 
TCTGTCACAT CACCGTACAA TGCGGATTTC GATGGAGATG AGATGAATCT TCATTTGCCA 
CAAAGTTTGG AGACCCGAGC CGAGATTGAG GAGATCGCTA TGGTGCCGAG ACAACTCATC 
ACTCCACAAG CCAACAAACC AGTCATGGGT ATCGTCCAGG ATACTCTCTG CGCCGTCCGT 
ATGATGACAA AACGTGACGT CTTCATTGAC TGGCCATTTA TGATGGATCT CCTTATGTAT 
CTACCAACTT GGGACGGAAA AGTCCCCCAA CCAGCAATCC TGAAGCCCAA ACCACTTTGG 
ACCGGAAAAC AAGTCTTCTC GCTTATCATT CCTGGAAATG TCAATGTTCT GAGAACCCAC 
AGTACCCATC CAGACTCTGA AGATTCTGGA CCATACAAAT GGATTTCCCC TGGAGATACC 
AAAGTTATCA TCGAACACGG AGAGCTTCTA TCGGGAATTG TGTGCTCCAA AACTGTCGGA 
AAATCGGCTG GTAACCTGCT CCACGTCGTC ACCCTTGAGC TCGGATACGA AATTGCCGCC 
AACTTTTATT CGCACATTCA AACGGTCATC AATGCATGGC TCATACGAGA AGGTCACACA 
ATCGGAATCG GTGATACGAT TGCCGATCAG GCGACCTATT TGGATATTCA GAATACTATC 
AGAAAAGCCA AACAGGATGT GGTGGATGTA ATTGAGAAGG CTCACAATGA TGATTTGGAG 
CCAACGCCCG GAAACACGTT GAGACAGACT TTTGAGAATA AAGTCAATCA GATTTTGAAC 
GACGCTCGTG ATCGAACGGG TAGTTCTGCG CAGAAGAGTT TGTCTGAATT CAACAATTTC 
AAGTCGATGG TGGTGTCAGG ATCGAAGGGA TCGAAGATTA ATATCTCGCA GGTTATCGCG 
TGTGTCGGAC AACAAAACGT CGAAGGAAAG CGTATTCCAT TCGGTTTCCG TCATCGTACA 
CTTCCTCACT TCATCAAGGA CGACTATGGA CCGGAATCCA AAGGTTTTGT CGAAAATTCC 
TATCTGGCCG GCCTAACTCC ATCGGAATTC TTCTTCCACG CAATGGGAGG ACGTGAAGGT 
CTGATTGATA CAGCTGTAAA GACTGCCGAG ACTGGATATA TTCAACGTCG TCTAATCAAG 
GCTATGGAAA GTGTAATGGT TAATTATGAT GGAACTGTGC GAAATTCCCT TGCACAAATG 
GTTCAATTGA GATATGGAGA AGATGGTTTG GATGGAATGT GGGTTGAGAA TCAGAATATG 
CCGACGATGA AGCCCAATAA TGCCGTGTTT GAGAGAGATT TCAGAATGGA CCTCACCGAC 
AACAAATTCC TTCGCAAAAA CTACTCGGAA GACGTTGTGC GCGAAATTCA AGAATCCGAA 
GACGGAATTT CGCTCGTCGA ATCGGAATGG TCACAGCTGG AAGAGGATCG TCGTCTCCTT 
CGAAAGATCT TTCCACGTGG TGACGCTAAG ATCGTGCTTC CGTGTAATCT GCAGCGTCTC 
ATTTGGAATG CTCAGAAAAT ATTCAAAGTT GATCTGCGCA AGCCGGTGAA TCTCTCGCCG 
TTGCACGTGA TCTCCGGAGT CCGTGAGCTT TCGAAAAAGC TGATCATTGT CAGTGGAAAC 
GACGAGATTT CAAAGCAGGC TCAGTACAAT GCGACACTTT TGATGAATAT CTTGCTCCGT 
TCGACACTTT GCACCAAGAA CATGTGCACA AAATCAAAAC TGAACTCTGA AGCGTTCGAT 
TGGCTCCTCG GAGAAATTGA ATCGCGATTC CAACAGGCTA TTGCTCAACC GGGAGAGATG 
GTTGGAGCAT TGGCGGCTCA ATCGCTCGGA GAGCCAGCTA CTCAGATGAC ACTCAACACG 
TTCCATTATG CAGGAGTTTC GGCGAAGAAT GTGACACTTG GAGTGCCGAG ATTGAAGGAG 
ATTATCAATG TTTCGAAGAC GTTGAAGACT CCGTCGTTGA CTGTATTCTT GACGGGAGCG 
GCTGCCAAGG ATCCGGAAAA GGCGAAGGAT GTGTTGTGCA AGTTGGAGCA TACCACGTTG 
AAAAAGGTAA CATGCAATAC AGCGATCTAC TACGATCCTG ACCCAAAGAA CACGGTGATC 
GCCGAAGACG AGGAATGGGT ATCGATTTTC TACGAGATGC CGGATCACGA TCTATCGCGT 
ACCTCTCCAT GGCTTCTTCG GATCGAATTG GACCGAAAAC GAATGGTCGA CAAGAAGTTG 
ACGATGGAAA TGATTGCCGA TCGGATTCAC GGAGGCTTCG GCAACGATGT TCACACTATC 
TACACCGACG ATAACGCCGA GAAGCTCGTT TTCCGTCTCC GAATCGCCGG AGAGGATAAG 
GGAGAAGCTC AGGAGGAGCA GGTGGATAAG ATGGAGGACG ACGTGTTCCT ACGATGTATC 
GAGGCAAATA TGTTGTCAGA TTTGACGCTT CAGGGAATCC CGGCGATCTC GAAGGTCTAC 
ATGAATCAGC CGAATACTGA TGATAAGAAG CGTATCATTA TTACACCGGA GGGAGGGTTC 
AAGTCGGTCG CCGACTGGAT CCTTGAAACT GATGGTACCG CGTTGCTCCG TGTGCTCTCC 
GAACGTCAGA TTGATCCGGT TCGAACGACA TCCAACGATA TCTGCGAGAT CTTTGAAGTG 
CTCGGAATCG AGGCGGTCAG AAAGGCTATC GAGCGAGAAA TGGACAATGT CATCTCGTTC 
GACGGATCCT ATGTCAATTA CCGACATTTG GCACTGCTTT GTGATGTGAT GACTGCGAAG 
GGACACTTGA TGGCTATTAC GAGACATGGA ATCAATCGTC AGGAGGTTGG AGCCCTGATG 
CGTTGTTCAT TCGAAGAAAC GGTGGATATT TTGATGGAGG CAGCCGTGCA TGCTGAGGAG 
GATCCTGTCA AGGGAGTGTC GGAGAATATT ATGCTCGGAC AGCTCGCTCG TTGTGGTACC 
GGATGCTTTG ATTTAGTATT GGACGTTGAA AAGTGCAAGT ATGGAATGGA AATCCCGCAG 
AATGTTGTAA TGGGCGGCGG GTTCTATGGA AGCTTTGCCG GCTCGCCGAG CAATCGCGAG 
TTCTCGCCGG CTCATTCGCC GTGGAACTCT GGAGTCACAC CGACGTATGC TGGAGCCGCC 
TGGTCGCCTA CCACAGGTGG AATGTCGCCT GGTGCTGGAT TTTCACCGGC TGGAAATACG 
GATGGAGGAG CATCGCCGTT CAATGAAGGA GGATGGTCTC CGGCATCGCC TGGGGATCCA 
CTGGGAGCAT TGTCTCCGCG TACTCCGTCG TATGGAGGAA TGTCACCTGG AGTCTACTCT 
CCATCGTCTC CGCAGTTCTC GATGACTTCA CCACACTATT CACCGACGTC TCCCAGTTAT 
TCGCCAACTT CCCCAGCCGC TGGACAATCG CCAGTGTCGC CGAGCTACTC GCCTACCTCG 
CCGAGCTATT CTCCGACTTC TCCCAGCTAT TCTCCGACAT CACCGAGCTA CTCGCCAACT 
TCTCCGAGCT ACTCGCCGAC GTCGCCGAGC TATTCTCCAA CATCGCCAAG TTATTCGCCG 
TCGTCGCCAA GCTATAGCCC TTCGTCGCCG AGCTACTCGC CTTCAAGCCC GAGATATTCG 
CCAACGTCGC CGACGTACAG TCCGACGAGT CCAACGTACT CTCCAACATC TCCAACGTAC 
TCGCCGACGT CGCCAACATA TTCTCCAACA TCTCCAAGTT ATGAAAGTGG AGGTGGATAC 
AGCCCATCGA GCCCCAAGTA CTCGCCGTCG TCGCCTACCT ATTCGCCTAC GTCGCCGAGC 
TACTCGCCAA CATCACCACA GTACTCGCCG ACGAGTCCGC AGTACAGTCC GAGCTCGCCG 
ACGTACACTC CATCCTCGCC GACATACAAT CCAACATCTC CACGCGGATT CTCGTCGCCA 
CAGTACTCTC CGACGTCGCC TACCTATTCG CCTACGTCGC CTAGCTACAC GCCGTCGTCG 
CCGCAGTACT CGCCGACGTC GCCTACCTAC ACTCCAAGTC CATCGGAGCA GCCAGGAACT 
TCGGCTCAGT ACTCTCCAAC ATCGCCAACG TACTCTCCGT CGTCTCCAAC GTATTCGCCA 
GCTTCACCAT CATACAGTCC AAGCTCGCCG ACTTATGATC CAAATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_500523.4
CDS	63..5633
Translation	MALVGVDFQAPLRIVSRVQFGILGPEEIKRMSVAHVEFPEVYENGKPKLGGLMDPRQGVIDRRGRCMTCAGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRLLIDKSAPRVLEILKKTGTNSKKRLTMIYDLCKAKSVCEGAAEKEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEWKKNVNEDTQERKIMLTAERVLEVFQQITDEDILVIGMDPQFARPEWMICTVLPVPPLAVRPAVVTFGSAKNQDDLTHKLSDIIKTNQQLQRNEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQKGGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTPFNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAADLHLQPGYRVERHMKDGDIIVFNRQPTLHKMSMMGHRVKILPWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLITPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMYLPTWDGKVPQPAILKPKPLWTGKQVFSLIIPGNVNVLRTHSTHPDSEDSGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVVTLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAHNDDLEPTPGNTLRQTFENKVNQILNDARDRTGSSAQKSLSEFNNFKSMVVSGSKGSKINISQVIACVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLDGMWVENQNMPTMKPNNAVFERDFRMDLTDNKFLRKNYSEDVVREIQESEDGISLVESEWSQLEEDRRLLRKIFPRGDAKIVLPCNLQRLIWNAQKIFKVDLRKPVNLSPLHVISGVRELSKKLIIVSGNDEISKQAQYNATLLMNILLRSTLCTKNMCTKSKLNSEAFDWLLGEIESRFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINVSKTLKTPSLTVFLTGAAAKDPEKAKDVLCKLEHTTLKKVTCNTAIYYDPDPKNTVIAEDEEWVSIFYEMPDHDLSRTSPWLLRIELDRKRMVDKKLTMEMIADRIHGGFGNDVHTIYTDDNAEKLVFRLRIAGEDKGEAQEEQVDKMEDDVFLRCIEANMLSDLTLQGIPAISKVYMNQPNTDDKKRIIITPEGGFKSVADWILETDGTALLRVLSERQIDPVRTTSNDICEIFEVLGIEAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETVDILMEAAVHAEEDPVKGVSENIMLGQLARCGTGCFDLVLDVEKCKYGMEIPQNVVMGGGFYGSFAGSPSNREFSPAHSPWNSGVTPTYAGAAWSPTTGGMSPGAGFSPAGNTDGGASPFNEGGWSPASPGDPLGALSPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSPRYSPTSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPSYSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSPQYSPTSPTYSPTSPSYTPSSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSPTYSPASPSYSPSSPTYDPNS

Target ORF information:

RefSeq Version	NM_068122.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB1 (ama-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068122.7

ORF Insert Sequence:

ATGGCTCTCG TCGGTGTCGA CTTCCAAGCG CCGCTGCGCA TTGTCTCACG CGTTCAGTTT 
GGAATTCTCG GCCCGGAGGA GATTAAACGC ATGTCAGTGG CTCATGTCGA GTTTCCAGAA 
GTCTACGAGA ACGGAAAGCC AAAGTTGGGC GGTCTCATGG ATCCAAGACA AGGAGTCATA 
GATCGTCGTG GAAGATGCAT GACATGCGCT GGAAATTTGA CAGATTGCCC GGGACATTTC 
GGTCATTTGG AGCTCGCCAA ACCCGTATTT CACATTGGAT TCCTCACAAA AACGCTGAAA 
ATACTGCGTT GCGTATGCTT CTACTGCGGA CGGCTTCTCA TCGACAAATC CGCTCCTCGA 
GTGCTGGAAA TTCTGAAAAA GACTGGCACA AACTCGAAAA AGCGGCTCAC AATGATCTAC 
GATCTGTGCA AAGCCAAGTC GGTGTGCGAA GGAGCAGCGG AGAAGGAAGA AGGAATGCCA 
GATGACCCGG ATGATCCGAT GAATGATGGA AAGAAGGTCG CAGGTGGATG CGGTCGCTAT 
CAACCATCAT ACCGCCGTGT CGGCATCGAC ATCAACGCAG AATGGAAGAA GAACGTGAAT 
GAGGACACGC AGGAGCGAAA AATCATGCTG ACCGCCGAAC GAGTACTCGA AGTCTTCCAA 
CAGATCACAG ACGAAGATAT TCTCGTAATA GGAATGGATC CACAATTCGC TCGGCCTGAA 
TGGATGATTT GTACCGTACT TCCAGTCCCA CCACTTGCAG TTCGTCCAGC AGTCGTCACA 
TTTGGATCGG CAAAGAATCA GGACGATTTG ACCCACAAAC TTTCGGATAT TATCAAAACC 
AATCAGCAGT TGCAGAGAAA TGAGGCTAAC GGAGCCGCAG CCCATGTGCT CACCGATGAC 
GTCAGGCTTT TGCAGTTCCA CGTGGCAACT CTTGTGGACA ATTGTATTCC GGGCCTGCCG 
ACGGCTACAC AGAAGGGTGG ACGACCGCTG AAATCGATCA AGCAGCGACT GAAAGGAAAA 
GAAGGACGCA TTCGTGGAAA TTTGATGGGA AAACGTGTGG ATTTCTCTGC GCGTACCGTC 
ATCACAGCCG ATCCGAACCT GCCGATTGAT ACGGTCGGTG TGCCGAGAAC TATTGCGCAG 
AATCTTACGT TCCCGGAAAT TGTGACACCT TTTAATGTGG ATAAGCTGCA GGAGTTGGTC 
AATCGTGGTG ATACACAGTA TCCTGGTGCC AAGTATATTA TTAGAGAAAA CGGAGCTCGA 
GTGGATCTTC GCTACCATCC GCGTGCCGCC GATCTTCACC TCCAACCGGG ATACCGTGTC 
GAACGTCACA TGAAAGATGG CGATATAATC GTCTTTAATC GTCAACCGAC TCTCCACAAA 
ATGTCAATGA TGGGACATCG TGTCAAAATC CTCCCATGGA GTACATTCCG AATGAATTTG 
TCTGTCACAT CACCGTACAA TGCGGATTTC GATGGAGATG AGATGAATCT TCATTTGCCA 
CAAAGTTTGG AGACCCGAGC CGAGATTGAG GAGATCGCTA TGGTGCCGAG ACAACTCATC 
ACTCCACAAG CCAACAAACC AGTCATGGGT ATCGTCCAGG ATACTCTCTG CGCCGTCCGT 
ATGATGACAA AACGTGACGT CTTCATTGAC TGGCCATTTA TGATGGATCT CCTTATGTAT 
CTACCAACTT GGGACGGAAA AGTCCCCCAA CCAGCAATCC TGAAGCCCAA ACCACTTTGG 
ACCGGAAAAC AAGTCTTCTC GCTTATCATT CCTGGAAATG TCAATGTTCT GAGAACCCAC 
AGTACCCATC CAGACTCTGA AGATTCTGGA CCATACAAAT GGATTTCCCC TGGAGATACC 
AAAGTTATCA TCGAACACGG AGAGCTTCTA TCGGGAATTG TGTGCTCCAA AACTGTCGGA 
AAATCGGCTG GTAACCTGCT CCACGTCGTC ACCCTTGAGC TCGGATACGA AATTGCCGCC 
AACTTTTATT CGCACATTCA AACGGTCATC AATGCATGGC TCATACGAGA AGGTCACACA 
ATCGGAATCG GTGATACGAT TGCCGATCAG GCGACCTATT TGGATATTCA GAATACTATC 
AGAAAAGCCA AACAGGATGT GGTGGATGTA ATTGAGAAGG CTCACAATGA TGATTTGGAG 
CCAACGCCCG GAAACACGTT GAGACAGACT TTTGAGAATA AAGTCAATCA GATTTTGAAC 
GACGCTCGTG ATCGAACGGG TAGTTCTGCG CAGAAGAGTT TGTCTGAATT CAACAATTTC 
AAGTCGATGG TGGTGTCAGG ATCGAAGGGA TCGAAGATTA ATATCTCGCA GGTTATCGCG 
TGTGTCGGAC AACAAAACGT CGAAGGAAAG CGTATTCCAT TCGGTTTCCG TCATCGTACA 
CTTCCTCACT TCATCAAGGA CGACTATGGA CCGGAATCCA AAGGTTTTGT CGAAAATTCC 
TATCTGGCCG GCCTAACTCC ATCGGAATTC TTCTTCCACG CAATGGGAGG ACGTGAAGGT 
CTGATTGATA CAGCTGTAAA GACTGCCGAG ACTGGATATA TTCAACGTCG TCTAATCAAG 
GCTATGGAAA GTGTAATGGT TAATTATGAT GGAACTGTGC GAAATTCCCT TGCACAAATG 
GTTCAATTGA GATATGGAGA AGATGGTTTG GATGGAATGT GGGTTGAGAA TCAGAATATG 
CCGACGATGA AGCCCAATAA TGCCGTGTTT GAGAGAGATT TCAGAATGGA CCTCACCGAC 
AACAAATTCC TTCGCAAAAA CTACTCGGAA GACGTTGTGC GCGAAATTCA AGAATCCGAA 
GACGGAATTT CGCTCGTCGA ATCGGAATGG TCACAGCTGG AAGAGGATCG TCGTCTCCTT 
CGAAAGATCT TTCCACGTGG TGACGCTAAG ATCGTGCTTC CGTGTAATCT GCAGCGTCTC 
ATTTGGAATG CTCAGAAAAT ATTCAAAGTT GATCTGCGCA AGCCGGTGAA TCTCTCGCCG 
TTGCACGTGA TCTCCGGAGT CCGTGAGCTT TCGAAAAAGC TGATCATTGT CAGTGGAAAC 
GACGAGATTT CAAAGCAGGC TCAGTACAAT GCGACACTTT TGATGAATAT CTTGCTCCGT 
TCGACACTTT GCACCAAGAA CATGTGCACA AAATCAAAAC TGAACTCTGA AGCGTTCGAT 
TGGCTCCTCG GAGAAATTGA ATCGCGATTC CAACAGGCTA TTGCTCAACC GGGAGAGATG 
GTTGGAGCAT TGGCGGCTCA ATCGCTCGGA GAGCCAGCTA CTCAGATGAC ACTCAACACG 
TTCCATTATG CAGGAGTTTC GGCGAAGAAT GTGACACTTG GAGTGCCGAG ATTGAAGGAG 
ATTATCAATG TTTCGAAGAC GTTGAAGACT CCGTCGTTGA CTGTATTCTT GACGGGAGCG 
GCTGCCAAGG ATCCGGAAAA GGCGAAGGAT GTGTTGTGCA AGTTGGAGCA TACCACGTTG 
AAAAAGGTAA CATGCAATAC AGCGATCTAC TACGATCCTG ACCCAAAGAA CACGGTGATC 
GCCGAAGACG AGGAATGGGT ATCGATTTTC TACGAGATGC CGGATCACGA TCTATCGCGT 
ACCTCTCCAT GGCTTCTTCG GATCGAATTG GACCGAAAAC GAATGGTCGA CAAGAAGTTG 
ACGATGGAAA TGATTGCCGA TCGGATTCAC GGAGGCTTCG GCAACGATGT TCACACTATC 
TACACCGACG ATAACGCCGA GAAGCTCGTT TTCCGTCTCC GAATCGCCGG AGAGGATAAG 
GGAGAAGCTC AGGAGGAGCA GGTGGATAAG ATGGAGGACG ACGTGTTCCT ACGATGTATC 
GAGGCAAATA TGTTGTCAGA TTTGACGCTT CAGGGAATCC CGGCGATCTC GAAGGTCTAC 
ATGAATCAGC CGAATACTGA TGATAAGAAG CGTATCATTA TTACACCGGA GGGAGGGTTC 
AAGTCGGTCG CCGACTGGAT CCTTGAAACT GATGGTACCG CGTTGCTCCG TGTGCTCTCC 
GAACGTCAGA TTGATCCGGT TCGAACGACA TCCAACGATA TCTGCGAGAT CTTTGAAGTG 
CTCGGAATCG AGGCGGTCAG AAAGGCTATC GAGCGAGAAA TGGACAATGT CATCTCGTTC 
GACGGATCCT ATGTCAATTA CCGACATTTG GCACTGCTTT GTGATGTGAT GACTGCGAAG 
GGACACTTGA TGGCTATTAC GAGACATGGA ATCAATCGTC AGGAGGTTGG AGCCCTGATG 
CGTTGTTCAT TCGAAGAAAC GGTGGATATT TTGATGGAGG CAGCCGTGCA TGCTGAGGAG 
GATCCTGTCA AGGGAGTGTC GGAGAATATT ATGCTCGGAC AGCTCGCTCG TTGTGGTACC 
GGATGCTTTG ATTTAGTATT GGACGTTGAA AAGTGCAAGT ATGGAATGGA AATCCCGCAG 
AATGTTGTAA TGGGCGGCGG GTTCTATGGA AGCTTTGCCG GCTCGCCGAG CAATCGCGAG 
TTCTCGCCGG CTCATTCGCC GTGGAACTCT GGAGTCACAC CGACGTATGC TGGAGCCGCC 
TGGTCGCCTA CCACAGGTGG AATGTCGCCT GGTGCTGGAT TTTCACCGGC TGGAAATACG 
GATGGAGGAG CATCGCCGTT CAATGAAGGA GGATGGTCTC CGGCATCGCC TGGGGATCCA 
CTGGGAGCAT TGTCTCCGCG TACTCCGTCG TATGGAGGAA TGTCACCTGG AGTCTACTCT 
CCATCGTCTC CGCAGTTCTC GATGACTTCA CCACACTATT CACCGACGTC TCCCAGTTAT 
TCGCCAACTT CCCCAGCCGC TGGACAATCG CCAGTGTCGC CGAGCTACTC GCCTACCTCG 
CCGAGCTATT CTCCGACTTC TCCCAGCTAT TCTCCGACAT CACCGAGCTA CTCGCCAACT 
TCTCCGAGCT ACTCGCCGAC GTCGCCGAGC TATTCTCCAA CATCGCCAAG TTATTCGCCG 
TCGTCGCCAA GCTATAGCCC TTCGTCGCCG AGCTACTCGC CTTCAAGCCC GAGATATTCG 
CCAACGTCGC CGACGTACAG TCCGACGAGT CCAACGTACT CTCCAACATC TCCAACGTAC 
TCGCCGACGT CGCCAACATA TTCTCCAACA TCTCCAAGTT ATGAAAGTGG AGGTGGATAC 
AGCCCATCGA GCCCCAAGTA CTCGCCGTCG TCGCCTACCT ATTCGCCTAC GTCGCCGAGC 
TACTCGCCAA CATCACCACA GTACTCGCCG ACGAGTCCGC AGTACAGTCC GAGCTCGCCG 
ACGTACACTC CATCCTCGCC GACATACAAT CCAACATCTC CACGCGGATT CTCGTCGCCA 
CAGTACTCTC CGACGTCGCC TACCTATTCG CCTACGTCGC CTAGCTACAC GCCGTCGTCG 
CCGCAGTACT CGCCGACGTC GCCTACCTAC ACTCCAAGTC CATCGGAGCA GCCAGGAACT 
TCGGCTCAGT ACTCTCCAAC ATCGCCAACG TACTCTCCGT CGTCTCCAAC GTATTCGCCA 
GCTTCACCAT CATACAGTCC AAGCTCGCCG ACTTATGATC CAAATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ama-1 ( NM_068122.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_500523.4 Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB1 (ama-1), partial mRNA.

Price & Availability↑

CloneID	OCa343837
Clone ID Related Accession (Same CDS sequence)	NM_068122.7 , NM_068122.6
Accession Version	NM_068122.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA-directed RNA polymerase II subunit RPB1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_068122.5. COMPLETENESS: incomplete on both ends.

ATGGCTCTCG TCGGTGTCGA CTTCCAAGCG CCGCTGCGCA TTGTCTCACG CGTTCAGTTT 
GGAATTCTCG GCCCGGAGGA GATTAAACGC ATGTCAGTGG CTCATGTCGA GTTTCCAGAA 
GTCTACGAGA ACGGAAAGCC AAAGTTGGGC GGTCTCATGG ATCCAAGACA AGGAGTCATA 
GATCGTCGTG GAAGATGCAT GACATGCGCT GGAAATTTGA CAGATTGCCC GGGACATTTC 
GGTCATTTGG AGCTCGCCAA ACCCGTATTT CACATTGGAT TCCTCACAAA AACGCTGAAA 
ATACTGCGTT GCGTATGCTT CTACTGCGGA CGGCTTCTCA TCGACAAATC CGCTCCTCGA 
GTGCTGGAAA TTCTGAAAAA GACTGGCACA AACTCGAAAA AGCGGCTCAC AATGATCTAC 
GATCTGTGCA AAGCCAAGTC GGTGTGCGAA GGAGCAGCGG AGAAGGAAGA AGGAATGCCA 
GATGACCCGG ATGATCCGAT GAATGATGGA AAGAAGGTCG CAGGTGGATG CGGTCGCTAT 
CAACCATCAT ACCGCCGTGT CGGCATCGAC ATCAACGCAG AATGGAAGAA GAACGTGAAT 
GAGGACACGC AGGAGCGAAA AATCATGCTG ACCGCCGAAC GAGTACTCGA AGTCTTCCAA 
CAGATCACAG ACGAAGATAT TCTCGTAATA GGAATGGATC CACAATTCGC TCGGCCTGAA 
TGGATGATTT GTACCGTACT TCCAGTCCCA CCACTTGCAG TTCGTCCAGC AGTCGTCACA 
TTTGGATCGG CAAAGAATCA GGACGATTTG ACCCACAAAC TTTCGGATAT TATCAAAACC 
AATCAGCAGT TGCAGAGAAA TGAGGCTAAC GGAGCCGCAG CCCATGTGCT CACCGATGAC 
GTCAGGCTTT TGCAGTTCCA CGTGGCAACT CTTGTGGACA ATTGTATTCC GGGCCTGCCG 
ACGGCTACAC AGAAGGGTGG ACGACCGCTG AAATCGATCA AGCAGCGACT GAAAGGAAAA 
GAAGGACGCA TTCGTGGAAA TTTGATGGGA AAACGTGTGG ATTTCTCTGC GCGTACCGTC 
ATCACAGCCG ATCCGAACCT GCCGATTGAT ACGGTCGGTG TGCCGAGAAC TATTGCGCAG 
AATCTTACGT TCCCGGAAAT TGTGACACCT TTTAATGTGG ATAAGCTGCA GGAGTTGGTC 
AATCGTGGTG ATACACAGTA TCCTGGTGCC AAGTATATTA TTAGAGAAAA CGGAGCTCGA 
GTGGATCTTC GCTACCATCC GCGTGCCGCC GATCTTCACC TCCAACCGGG ATACCGTGTC 
GAACGTCACA TGAAAGATGG CGATATAATC GTCTTTAATC GTCAACCGAC TCTCCACAAA 
ATGTCAATGA TGGGACATCG TGTCAAAATC CTCCCATGGA GTACATTCCG AATGAATTTG 
TCTGTCACAT CACCGTACAA TGCGGATTTC GATGGAGATG AGATGAATCT TCATTTGCCA 
CAAAGTTTGG AGACCCGAGC CGAGATTGAG GAGATCGCTA TGGTGCCGAG ACAACTCATC 
ACTCCACAAG CCAACAAACC AGTCATGGGT ATCGTCCAGG ATACTCTCTG CGCCGTCCGT 
ATGATGACAA AACGTGACGT CTTCATTGAC TGGCCATTTA TGATGGATCT CCTTATGTAT 
CTACCAACTT GGGACGGAAA AGTCCCCCAA CCAGCAATCC TGAAGCCCAA ACCACTTTGG 
ACCGGAAAAC AAGTCTTCTC GCTTATCATT CCTGGAAATG TCAATGTTCT GAGAACCCAC 
AGTACCCATC CAGACTCTGA AGATTCTGGA CCATACAAAT GGATTTCCCC TGGAGATACC 
AAAGTTATCA TCGAACACGG AGAGCTTCTA TCGGGAATTG TGTGCTCCAA AACTGTCGGA 
AAATCGGCTG GTAACCTGCT CCACGTCGTC ACCCTTGAGC TCGGATACGA AATTGCCGCC 
AACTTTTATT CGCACATTCA AACGGTCATC AATGCATGGC TCATACGAGA AGGTCACACA 
ATCGGAATCG GTGATACGAT TGCCGATCAG GCGACCTATT TGGATATTCA GAATACTATC 
AGAAAAGCCA AACAGGATGT GGTGGATGTA ATTGAGAAGG CTCACAATGA TGATTTGGAG 
CCAACGCCCG GAAACACGTT GAGACAGACT TTTGAGAATA AAGTCAATCA GATTTTGAAC 
GACGCTCGTG ATCGAACGGG TAGTTCTGCG CAGAAGAGTT TGTCTGAATT CAACAATTTC 
AAGTCGATGG TGGTGTCAGG ATCGAAGGGA TCGAAGATTA ATATCTCGCA GGTTATCGCG 
TGTGTCGGAC AACAAAACGT CGAAGGAAAG CGTATTCCAT TCGGTTTCCG TCATCGTACA 
CTTCCTCACT TCATCAAGGA CGACTATGGA CCGGAATCCA AAGGTTTTGT CGAAAATTCC 
TATCTGGCCG GCCTAACTCC ATCGGAATTC TTCTTCCACG CAATGGGAGG ACGTGAAGGT 
CTGATTGATA CAGCTGTAAA GACTGCCGAG ACTGGATATA TTCAACGTCG TCTAATCAAG 
GCTATGGAAA GTGTAATGGT TAATTATGAT GGAACTGTGC GAAATTCCCT TGCACAAATG 
GTTCAATTGA GATATGGAGA AGATGGTTTG GATGGAATGT GGGTTGAGAA TCAGAATATG 
CCGACGATGA AGCCCAATAA TGCCGTGTTT GAGAGAGATT TCAGAATGGA CCTCACCGAC 
AACAAATTCC TTCGCAAAAA CTACTCGGAA GACGTTGTGC GCGAAATTCA AGAATCCGAA 
GACGGAATTT CGCTCGTCGA ATCGGAATGG TCACAGCTGG AAGAGGATCG TCGTCTCCTT 
CGAAAGATCT TTCCACGTGG TGACGCTAAG ATCGTGCTTC CGTGTAATCT GCAGCGTCTC 
ATTTGGAATG CTCAGAAAAT ATTCAAAGTT GATCTGCGCA AGCCGGTGAA TCTCTCGCCG 
TTGCACGTGA TCTCCGGAGT CCGTGAGCTT TCGAAAAAGC TGATCATTGT CAGTGGAAAC 
GACGAGATTT CAAAGCAGGC TCAGTACAAT GCGACACTTT TGATGAATAT CTTGCTCCGT 
TCGACACTTT GCACCAAGAA CATGTGCACA AAATCAAAAC TGAACTCTGA AGCGTTCGAT 
TGGCTCCTCG GAGAAATTGA ATCGCGATTC CAACAGGCTA TTGCTCAACC GGGAGAGATG 
GTTGGAGCAT TGGCGGCTCA ATCGCTCGGA GAGCCAGCTA CTCAGATGAC ACTCAACACG 
TTCCATTATG CAGGAGTTTC GGCGAAGAAT GTGACACTTG GAGTGCCGAG ATTGAAGGAG 
ATTATCAATG TTTCGAAGAC GTTGAAGACT CCGTCGTTGA CTGTATTCTT GACGGGAGCG 
GCTGCCAAGG ATCCGGAAAA GGCGAAGGAT GTGTTGTGCA AGTTGGAGCA TACCACGTTG 
AAAAAGGTAA CATGCAATAC AGCGATCTAC TACGATCCTG ACCCAAAGAA CACGGTGATC 
GCCGAAGACG AGGAATGGGT ATCGATTTTC TACGAGATGC CGGATCACGA TCTATCGCGT 
ACCTCTCCAT GGCTTCTTCG GATCGAATTG GACCGAAAAC GAATGGTCGA CAAGAAGTTG 
ACGATGGAAA TGATTGCCGA TCGGATTCAC GGAGGCTTCG GCAACGATGT TCACACTATC 
TACACCGACG ATAACGCCGA GAAGCTCGTT TTCCGTCTCC GAATCGCCGG AGAGGATAAG 
GGAGAAGCTC AGGAGGAGCA GGTGGATAAG ATGGAGGACG ACGTGTTCCT ACGATGTATC 
GAGGCAAATA TGTTGTCAGA TTTGACGCTT CAGGGAATCC CGGCGATCTC GAAGGTCTAC 
ATGAATCAGC CGAATACTGA TGATAAGAAG CGTATCATTA TTACACCGGA GGGAGGGTTC 
AAGTCGGTCG CCGACTGGAT CCTTGAAACT GATGGTACCG CGTTGCTCCG TGTGCTCTCC 
GAACGTCAGA TTGATCCGGT TCGAACGACA TCCAACGATA TCTGCGAGAT CTTTGAAGTG 
CTCGGAATCG AGGCGGTCAG AAAGGCTATC GAGCGAGAAA TGGACAATGT CATCTCGTTC 
GACGGATCCT ATGTCAATTA CCGACATTTG GCACTGCTTT GTGATGTGAT GACTGCGAAG 
GGACACTTGA TGGCTATTAC GAGACATGGA ATCAATCGTC AGGAGGTTGG AGCCCTGATG 
CGTTGTTCAT TCGAAGAAAC GGTGGATATT TTGATGGAGG CAGCCGTGCA TGCTGAGGAG 
GATCCTGTCA AGGGAGTGTC GGAGAATATT ATGCTCGGAC AGCTCGCTCG TTGTGGTACC 
GGATGCTTTG ATTTAGTATT GGACGTTGAA AAGTGCAAGT ATGGAATGGA AATCCCGCAG 
AATGTTGTAA TGGGCGGCGG GTTCTATGGA AGCTTTGCCG GCTCGCCGAG CAATCGCGAG 
TTCTCGCCGG CTCATTCGCC GTGGAACTCT GGAGTCACAC CGACGTATGC TGGAGCCGCC 
TGGTCGCCTA CCACAGGTGG AATGTCGCCT GGTGCTGGAT TTTCACCGGC TGGAAATACG 
GATGGAGGAG CATCGCCGTT CAATGAAGGA GGATGGTCTC CGGCATCGCC TGGGGATCCA 
CTGGGAGCAT TGTCTCCGCG TACTCCGTCG TATGGAGGAA TGTCACCTGG AGTCTACTCT 
CCATCGTCTC CGCAGTTCTC GATGACTTCA CCACACTATT CACCGACGTC TCCCAGTTAT 
TCGCCAACTT CCCCAGCCGC TGGACAATCG CCAGTGTCGC CGAGCTACTC GCCTACCTCG 
CCGAGCTATT CTCCGACTTC TCCCAGCTAT TCTCCGACAT CACCGAGCTA CTCGCCAACT 
TCTCCGAGCT ACTCGCCGAC GTCGCCGAGC TATTCTCCAA CATCGCCAAG TTATTCGCCG 
TCGTCGCCAA GCTATAGCCC TTCGTCGCCG AGCTACTCGC CTTCAAGCCC GAGATATTCG 
CCAACGTCGC CGACGTACAG TCCGACGAGT CCAACGTACT CTCCAACATC TCCAACGTAC 
TCGCCGACGT CGCCAACATA TTCTCCAACA TCTCCAAGTT ATGAAAGTGG AGGTGGATAC 
AGCCCATCGA GCCCCAAGTA CTCGCCGTCG TCGCCTACCT ATTCGCCTAC GTCGCCGAGC 
TACTCGCCAA CATCACCACA GTACTCGCCG ACGAGTCCGC AGTACAGTCC GAGCTCGCCG 
ACGTACACTC CATCCTCGCC GACATACAAT CCAACATCTC CACGCGGATT CTCGTCGCCA 
CAGTACTCTC CGACGTCGCC TACCTATTCG CCTACGTCGC CTAGCTACAC GCCGTCGTCG 
CCGCAGTACT CGCCGACGTC GCCTACCTAC ACTCCAAGTC CATCGGAGCA GCCAGGAACT 
TCGGCTCAGT ACTCTCCAAC ATCGCCAACG TACTCTCCGT CGTCTCCAAC GTATTCGCCA 
GCTTCACCAT CATACAGTCC AAGCTCGCCG ACTTATGATC CAAATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_500523.4
CDS	1..5571
Translation	MALVGVDFQAPLRIVSRVQFGILGPEEIKRMSVAHVEFPEVYENGKPKLGGLMDPRQGVIDRRGRCMTCAGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRLLIDKSAPRVLEILKKTGTNSKKRLTMIYDLCKAKSVCEGAAEKEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEWKKNVNEDTQERKIMLTAERVLEVFQQITDEDILVIGMDPQFARPEWMICTVLPVPPLAVRPAVVTFGSAKNQDDLTHKLSDIIKTNQQLQRNEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQKGGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTPFNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAADLHLQPGYRVERHMKDGDIIVFNRQPTLHKMSMMGHRVKILPWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLITPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMYLPTWDGKVPQPAILKPKPLWTGKQVFSLIIPGNVNVLRTHSTHPDSEDSGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVVTLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAHNDDLEPTPGNTLRQTFENKVNQILNDARDRTGSSAQKSLSEFNNFKSMVVSGSKGSKINISQVIACVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLDGMWVENQNMPTMKPNNAVFERDFRMDLTDNKFLRKNYSEDVVREIQESEDGISLVESEWSQLEEDRRLLRKIFPRGDAKIVLPCNLQRLIWNAQKIFKVDLRKPVNLSPLHVISGVRELSKKLIIVSGNDEISKQAQYNATLLMNILLRSTLCTKNMCTKSKLNSEAFDWLLGEIESRFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINVSKTLKTPSLTVFLTGAAAKDPEKAKDVLCKLEHTTLKKVTCNTAIYYDPDPKNTVIAEDEEWVSIFYEMPDHDLSRTSPWLLRIELDRKRMVDKKLTMEMIADRIHGGFGNDVHTIYTDDNAEKLVFRLRIAGEDKGEAQEEQVDKMEDDVFLRCIEANMLSDLTLQGIPAISKVYMNQPNTDDKKRIIITPEGGFKSVADWILETDGTALLRVLSERQIDPVRTTSNDICEIFEVLGIEAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETVDILMEAAVHAEEDPVKGVSENIMLGQLARCGTGCFDLVLDVEKCKYGMEIPQNVVMGGGFYGSFAGSPSNREFSPAHSPWNSGVTPTYAGAAWSPTTGGMSPGAGFSPAGNTDGGASPFNEGGWSPASPGDPLGALSPRTPSYGGMSPGVYSPSSPQFSMTSPHYSPTSPSYSPTSPAAGQSPVSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSSPSYSPSSPRYSPTSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPSYESGGGYSPSSPKYSPSSPTYSPTSPSYSPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSPRGFSSPQYSPTSPTYSPTSPSYTPSSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYSPSSPTYSPASPSYSPSSPTYDPNS

Target ORF information:

RefSeq Version	NM_068122.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA-directed RNA polymerase II subunit RPB1 (ama-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068122.6

ORF Insert Sequence:

ATGGCTCTCG TCGGTGTCGA CTTCCAAGCG CCGCTGCGCA TTGTCTCACG CGTTCAGTTT 
GGAATTCTCG GCCCGGAGGA GATTAAACGC ATGTCAGTGG CTCATGTCGA GTTTCCAGAA 
GTCTACGAGA ACGGAAAGCC AAAGTTGGGC GGTCTCATGG ATCCAAGACA AGGAGTCATA 
GATCGTCGTG GAAGATGCAT GACATGCGCT GGAAATTTGA CAGATTGCCC GGGACATTTC 
GGTCATTTGG AGCTCGCCAA ACCCGTATTT CACATTGGAT TCCTCACAAA AACGCTGAAA 
ATACTGCGTT GCGTATGCTT CTACTGCGGA CGGCTTCTCA TCGACAAATC CGCTCCTCGA 
GTGCTGGAAA TTCTGAAAAA GACTGGCACA AACTCGAAAA AGCGGCTCAC AATGATCTAC 
GATCTGTGCA AAGCCAAGTC GGTGTGCGAA GGAGCAGCGG AGAAGGAAGA AGGAATGCCA 
GATGACCCGG ATGATCCGAT GAATGATGGA AAGAAGGTCG CAGGTGGATG CGGTCGCTAT 
CAACCATCAT ACCGCCGTGT CGGCATCGAC ATCAACGCAG AATGGAAGAA GAACGTGAAT 
GAGGACACGC AGGAGCGAAA AATCATGCTG ACCGCCGAAC GAGTACTCGA AGTCTTCCAA 
CAGATCACAG ACGAAGATAT TCTCGTAATA GGAATGGATC CACAATTCGC TCGGCCTGAA 
TGGATGATTT GTACCGTACT TCCAGTCCCA CCACTTGCAG TTCGTCCAGC AGTCGTCACA 
TTTGGATCGG CAAAGAATCA GGACGATTTG ACCCACAAAC TTTCGGATAT TATCAAAACC 
AATCAGCAGT TGCAGAGAAA TGAGGCTAAC GGAGCCGCAG CCCATGTGCT CACCGATGAC 
GTCAGGCTTT TGCAGTTCCA CGTGGCAACT CTTGTGGACA ATTGTATTCC GGGCCTGCCG 
ACGGCTACAC AGAAGGGTGG ACGACCGCTG AAATCGATCA AGCAGCGACT GAAAGGAAAA 
GAAGGACGCA TTCGTGGAAA TTTGATGGGA AAACGTGTGG ATTTCTCTGC GCGTACCGTC 
ATCACAGCCG ATCCGAACCT GCCGATTGAT ACGGTCGGTG TGCCGAGAAC TATTGCGCAG 
AATCTTACGT TCCCGGAAAT TGTGACACCT TTTAATGTGG ATAAGCTGCA GGAGTTGGTC 
AATCGTGGTG ATACACAGTA TCCTGGTGCC AAGTATATTA TTAGAGAAAA CGGAGCTCGA 
GTGGATCTTC GCTACCATCC GCGTGCCGCC GATCTTCACC TCCAACCGGG ATACCGTGTC 
GAACGTCACA TGAAAGATGG CGATATAATC GTCTTTAATC GTCAACCGAC TCTCCACAAA 
ATGTCAATGA TGGGACATCG TGTCAAAATC CTCCCATGGA GTACATTCCG AATGAATTTG 
TCTGTCACAT CACCGTACAA TGCGGATTTC GATGGAGATG AGATGAATCT TCATTTGCCA 
CAAAGTTTGG AGACCCGAGC CGAGATTGAG GAGATCGCTA TGGTGCCGAG ACAACTCATC 
ACTCCACAAG CCAACAAACC AGTCATGGGT ATCGTCCAGG ATACTCTCTG CGCCGTCCGT 
ATGATGACAA AACGTGACGT CTTCATTGAC TGGCCATTTA TGATGGATCT CCTTATGTAT 
CTACCAACTT GGGACGGAAA AGTCCCCCAA CCAGCAATCC TGAAGCCCAA ACCACTTTGG 
ACCGGAAAAC AAGTCTTCTC GCTTATCATT CCTGGAAATG TCAATGTTCT GAGAACCCAC 
AGTACCCATC CAGACTCTGA AGATTCTGGA CCATACAAAT GGATTTCCCC TGGAGATACC 
AAAGTTATCA TCGAACACGG AGAGCTTCTA TCGGGAATTG TGTGCTCCAA AACTGTCGGA 
AAATCGGCTG GTAACCTGCT CCACGTCGTC ACCCTTGAGC TCGGATACGA AATTGCCGCC 
AACTTTTATT CGCACATTCA AACGGTCATC AATGCATGGC TCATACGAGA AGGTCACACA 
ATCGGAATCG GTGATACGAT TGCCGATCAG GCGACCTATT TGGATATTCA GAATACTATC 
AGAAAAGCCA AACAGGATGT GGTGGATGTA ATTGAGAAGG CTCACAATGA TGATTTGGAG 
CCAACGCCCG GAAACACGTT GAGACAGACT TTTGAGAATA AAGTCAATCA GATTTTGAAC 
GACGCTCGTG ATCGAACGGG TAGTTCTGCG CAGAAGAGTT TGTCTGAATT CAACAATTTC 
AAGTCGATGG TGGTGTCAGG ATCGAAGGGA TCGAAGATTA ATATCTCGCA GGTTATCGCG 
TGTGTCGGAC AACAAAACGT CGAAGGAAAG CGTATTCCAT TCGGTTTCCG TCATCGTACA 
CTTCCTCACT TCATCAAGGA CGACTATGGA CCGGAATCCA AAGGTTTTGT CGAAAATTCC 
TATCTGGCCG GCCTAACTCC ATCGGAATTC TTCTTCCACG CAATGGGAGG ACGTGAAGGT 
CTGATTGATA CAGCTGTAAA GACTGCCGAG ACTGGATATA TTCAACGTCG TCTAATCAAG 
GCTATGGAAA GTGTAATGGT TAATTATGAT GGAACTGTGC GAAATTCCCT TGCACAAATG 
GTTCAATTGA GATATGGAGA AGATGGTTTG GATGGAATGT GGGTTGAGAA TCAGAATATG 
CCGACGATGA AGCCCAATAA TGCCGTGTTT GAGAGAGATT TCAGAATGGA CCTCACCGAC 
AACAAATTCC TTCGCAAAAA CTACTCGGAA GACGTTGTGC GCGAAATTCA AGAATCCGAA 
GACGGAATTT CGCTCGTCGA ATCGGAATGG TCACAGCTGG AAGAGGATCG TCGTCTCCTT 
CGAAAGATCT TTCCACGTGG TGACGCTAAG ATCGTGCTTC CGTGTAATCT GCAGCGTCTC 
ATTTGGAATG CTCAGAAAAT ATTCAAAGTT GATCTGCGCA AGCCGGTGAA TCTCTCGCCG 
TTGCACGTGA TCTCCGGAGT CCGTGAGCTT TCGAAAAAGC TGATCATTGT CAGTGGAAAC 
GACGAGATTT CAAAGCAGGC TCAGTACAAT GCGACACTTT TGATGAATAT CTTGCTCCGT 
TCGACACTTT GCACCAAGAA CATGTGCACA AAATCAAAAC TGAACTCTGA AGCGTTCGAT 
TGGCTCCTCG GAGAAATTGA ATCGCGATTC CAACAGGCTA TTGCTCAACC GGGAGAGATG 
GTTGGAGCAT TGGCGGCTCA ATCGCTCGGA GAGCCAGCTA CTCAGATGAC ACTCAACACG 
TTCCATTATG CAGGAGTTTC GGCGAAGAAT GTGACACTTG GAGTGCCGAG ATTGAAGGAG 
ATTATCAATG TTTCGAAGAC GTTGAAGACT CCGTCGTTGA CTGTATTCTT GACGGGAGCG 
GCTGCCAAGG ATCCGGAAAA GGCGAAGGAT GTGTTGTGCA AGTTGGAGCA TACCACGTTG 
AAAAAGGTAA CATGCAATAC AGCGATCTAC TACGATCCTG ACCCAAAGAA CACGGTGATC 
GCCGAAGACG AGGAATGGGT ATCGATTTTC TACGAGATGC CGGATCACGA TCTATCGCGT 
ACCTCTCCAT GGCTTCTTCG GATCGAATTG GACCGAAAAC GAATGGTCGA CAAGAAGTTG 
ACGATGGAAA TGATTGCCGA TCGGATTCAC GGAGGCTTCG GCAACGATGT TCACACTATC 
TACACCGACG ATAACGCCGA GAAGCTCGTT TTCCGTCTCC GAATCGCCGG AGAGGATAAG 
GGAGAAGCTC AGGAGGAGCA GGTGGATAAG ATGGAGGACG ACGTGTTCCT ACGATGTATC 
GAGGCAAATA TGTTGTCAGA TTTGACGCTT CAGGGAATCC CGGCGATCTC GAAGGTCTAC 
ATGAATCAGC CGAATACTGA TGATAAGAAG CGTATCATTA TTACACCGGA GGGAGGGTTC 
AAGTCGGTCG CCGACTGGAT CCTTGAAACT GATGGTACCG CGTTGCTCCG TGTGCTCTCC 
GAACGTCAGA TTGATCCGGT TCGAACGACA TCCAACGATA TCTGCGAGAT CTTTGAAGTG 
CTCGGAATCG AGGCGGTCAG AAAGGCTATC GAGCGAGAAA TGGACAATGT CATCTCGTTC 
GACGGATCCT ATGTCAATTA CCGACATTTG GCACTGCTTT GTGATGTGAT GACTGCGAAG 
GGACACTTGA TGGCTATTAC GAGACATGGA ATCAATCGTC AGGAGGTTGG AGCCCTGATG 
CGTTGTTCAT TCGAAGAAAC GGTGGATATT TTGATGGAGG CAGCCGTGCA TGCTGAGGAG 
GATCCTGTCA AGGGAGTGTC GGAGAATATT ATGCTCGGAC AGCTCGCTCG TTGTGGTACC 
GGATGCTTTG ATTTAGTATT GGACGTTGAA AAGTGCAAGT ATGGAATGGA AATCCCGCAG 
AATGTTGTAA TGGGCGGCGG GTTCTATGGA AGCTTTGCCG GCTCGCCGAG CAATCGCGAG 
TTCTCGCCGG CTCATTCGCC GTGGAACTCT GGAGTCACAC CGACGTATGC TGGAGCCGCC 
TGGTCGCCTA CCACAGGTGG AATGTCGCCT GGTGCTGGAT TTTCACCGGC TGGAAATACG 
GATGGAGGAG CATCGCCGTT CAATGAAGGA GGATGGTCTC CGGCATCGCC TGGGGATCCA 
CTGGGAGCAT TGTCTCCGCG TACTCCGTCG TATGGAGGAA TGTCACCTGG AGTCTACTCT 
CCATCGTCTC CGCAGTTCTC GATGACTTCA CCACACTATT CACCGACGTC TCCCAGTTAT 
TCGCCAACTT CCCCAGCCGC TGGACAATCG CCAGTGTCGC CGAGCTACTC GCCTACCTCG 
CCGAGCTATT CTCCGACTTC TCCCAGCTAT TCTCCGACAT CACCGAGCTA CTCGCCAACT 
TCTCCGAGCT ACTCGCCGAC GTCGCCGAGC TATTCTCCAA CATCGCCAAG TTATTCGCCG 
TCGTCGCCAA GCTATAGCCC TTCGTCGCCG AGCTACTCGC CTTCAAGCCC GAGATATTCG 
CCAACGTCGC CGACGTACAG TCCGACGAGT CCAACGTACT CTCCAACATC TCCAACGTAC 
TCGCCGACGT CGCCAACATA TTCTCCAACA TCTCCAAGTT ATGAAAGTGG AGGTGGATAC 
AGCCCATCGA GCCCCAAGTA CTCGCCGTCG TCGCCTACCT ATTCGCCTAC GTCGCCGAGC 
TACTCGCCAA CATCACCACA GTACTCGCCG ACGAGTCCGC AGTACAGTCC GAGCTCGCCG 
ACGTACACTC CATCCTCGCC GACATACAAT CCAACATCTC CACGCGGATT CTCGTCGCCA 
CAGTACTCTC CGACGTCGCC TACCTATTCG CCTACGTCGC CTAGCTACAC GCCGTCGTCG 
CCGCAGTACT CGCCGACGTC GCCTACCTAC ACTCCAAGTC CATCGGAGCA GCCAGGAACT 
TCGGCTCAGT ACTCTCCAAC ATCGCCAACG TACTCTCCGT CGTCTCCAAC GTATTCGCCA 
GCTTCACCAT CATACAGTCC AAGCTCGCCG ACTTATGATC CAAATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ama-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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