NCU02103 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02103
Entrez Gene ID	3880246
Full Name	DNA-directed RNA polymerase II largest subunit
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959004.2	XP_964097.2	DNA-directed RNA polymerase II largest subunit

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Homo sapiens (human)	POLR2A	NP_000928.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02103 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02103 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12686	XM_959004.2 Latest version!	Neurospora crassa OR74A DNA-directed RNA polymerase II largest subunit (NCU02103), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02103 ( XM_959004.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964097.2 Neurospora crassa OR74A DNA-directed RNA polymerase II largest subunit (NCU02103), mRNA.

Price & Availability↑

CloneID	ONf12686
Clone ID Related Accession (Same CDS sequence)	XM_959004.2
Accession Version	XM_959004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA-directed RNA polymerase II largest subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959004.1.

ATGGCGAACC TATACTTTGC GCACTCGAGT GCGCCCTTGC GGACCATCAA GGAAATCCAG 
TTTGGTCTGC TGTCGCCCGA GGAGATCAAG GGCATGAGTG TGGCCCACAT CGTCTACCCA 
GAGACCATGG ACGAGACCAG GACAAAACCC CGCGACGGCG GTCTCAACGA CCCTCTCCTG 
GGCTCCGTAG ATCGTCAGTT CAAGTGCAAG ACGTGCGGCG AGAACATGTC CGAGTGCCCC 
GGCCATTTCG GTCACATCGA GCTGGCCCGA CCCGTCTACC ATCCGGGCTT CATCAAGCGG 
GTGAAGAAGA TGTTGGAGAT TGTCTGCCAC AACTGCAGCA AGGTTCTTGC TGACAGAAAT 
GACAAAGAGT TCGCGGCCGC CATGAGGATC CGCGATCCCA AGGTCCGCTT CAACCGAGTA 
TGGGCTGTGT GCAAGAAAAA GAGGAGATGT GAAAACGAGG TAGTCAAGAA GAGCGACGAT 
GACGAGTTCA ACCCTCTTGC CGACGCCAAG GGCGAGCTCC CCGGCCACGG CGGCTGCGGC 
AACGATCACC CCACCGTCCG CCAGCAGGCC CTCACCCTCT ATGCCCAGTT CGATACCAAG 
GACGAGGAGG GCGTCAAGAG CAAGGAGAAG AAGATCATCA CCCCCGAGAT GGCACTCAAC 
ATTTTGCACA ACATGACCGA GTCTGACATG ATCGACATCG GCATCAACAT CTCTCAAGCT 
CGGCCTGAGT GGATGATCAT CACTGTTCTG CCTGTTCCGC CCCCTCCAGT CCGCCCCAGT 
ATTTCCATGG ACGGTACCGG CACCGGTATG CGGAACGAAG ATGATTTGAC CTACAAGCTC 
GGCGATATCA TTCGAGCAAA CGGCAACGTT CGCCAGGCCA TCCAGGAGGG TTCTCCGGCT 
CACATTATCA ACGATTTCGA GAACCTCCTC CAGTACCACG TTGCTACCTA CATGGACAAC 
GATATTGCTG GTCAACCACA AGCTCTGCAA AAGAGCGGTC GTCCCGTCAA GGCCATTCGT 
GCCCGTCTCA AGGGCAAGGA GGGTCGTCTG CGTGGTAACT TGATGGGCAA GCGTGTCGAT 
TTCTCTGCCC GTACAGTCAT CACAGGCGAT GCCAACATCT CGCTGGATGA AGTCGGTGTG 
CCCCGTAGCA TCGCGCGTAC GCTTACCTAT CCCGAGACTG TGACGCCCTA CAATATTGGC 
AAACTCACCC AATACGTCCA GAACGGCCCT AACGAGCATC CGGGTGCCAA GTACGTTATC 
CGCTCAGATG GCAGCCGTGT CGATCTACGC CATCAGAGGC GGACAGGTCC GCTACAGCTG 
GAATATGGCT GGAAGGTTGA GCGCCATCTC ATCGACGGTG ATTACATCAT CTTCAACCGT 
CAGCCCTCTT TGCACAAGGA ATCCATGATG GGTCATCGTG TCAAGGTCAT GCCCTACTCG 
ACCTTCAGAC TTAACCTTTC TGTCACATCT CCTTACAACG CCGATTTCGA CGGTGATGAA 
ATGAACCTTC ACGTTCCTCA AACCGAAGAA ACTCGTGCTG AAGTCAAGGA GCTTTGCATG 
GTTCCTCTCA ACATCGTCTC GCCGCAGCGT AACGGTCCGC TCATGGGTAT CGTGCAGGAC 
ACTTTGGCTG GTGCTTACAA GCTTTGTCGT CGTGACGTCT TCCTTACCAA GGAGGAGGTC 
ATGAACATTA TGCTCTGGGT CCCCGAGTGG GACGGCATCA TTCCTGTCCC TGCAATCCTC 
AAGCCTCGTC CTCGCTGGAC TGGTAAGCAA ATCATTAGCA TGGTTGTTCC CAAGATCATA 
AACCTCAAGA ACATCGACGA GGATGATGGT GAGTGCCCTC TTAAGGACAA GGGCATTTTG 
ATCCAACAAG GTGAGGTGAT TTTCGGCTTG ATGACGAAGA AGATTGTGGG TGCTGCTAGT 
GGCGGTATCG TCCATCTCTG CTACAACGAG CTGGGTCCTA GCGGTGCTAT GGATTTCCTC 
AACGGCTGCC AGAGAGTCGT CAACTACTGG CTCCTTCACA ATGGTCACAG CATTGGTATT 
GGTGACACTA TTCCCGACGC CGCGACCATC GCCAAGGTCC AGACGCACAT TGACGATGCC 
AAGGCAGAAG TCAAGCAGCT CACAGATATG GCCACCAACA ATCAGCTTGA GCCATTGCCC 
GGTATGAACA TCCGTGAGAC CTTCGAAAAC AAGGTCTCCA AGGCTCTCAA CACGGCCCGT 
GACAAGGCCG GTACTACCAC TGAGAGCAGT TTGAAGGATA TCAACAACGC CGTAACCATG 
GCTCTGTCTG GTTCCAAGGG TTCCTCCATC AACATTTCCC AGATGACTGC CTTGGTCGGT 
CAGCAGATTG TCGAAGGCAA GCGTATTCCT TTCGGCTTCA AGTACCGCAC ACTGCCACAT 
TTCACAAAGG ATGATTACTC GCCCGAAGCC CGTGGTTTCG TCGAGAACTC GTACCTTCGT 
GGTCTTACTC CTTCAGAGTT TTTCTTCCAC GCCATGGCTG GTAGAGAAGG TTTGATCGAT 
ACCGCTGTCA AGACAGCCGA AACTGGTTAC ATTCAGCGTC GTTTGGTCAA GGCCCTTGAA 
GATTCGGCTG CGCAGTACGA TGGTACTGTT CGCAACTCGC TGGGTGATAT CATCCAGTTC 
GTCTACGGTG AAGATGGTCT CGACGGTATT GCCATCGAGA ACCAGCCTGT CGACCACTTC 
ACGATATCCA ACAAGGCTTT CGACAAGCGT TATCGTCTCG ATGTCATGGA CGAGGAGGTT 
TCCTCGGGCG AGCTTGAGGC GCTGGAATAC GGAAAGGAGC TTGCTAGTGA CCCCACTGTT 
CAGGAGCTTC TTGACCAGGA GTACGAACAG CTTTCGGTCG ATCGCCACAT GCTTCGCGAG 
ATCCAGCGCA CAAAAGGCAA CAATGACACG AAGATGCAGC TCCCGCTTAA CATCATTCGT 
ATTATTGATA CTGCCAAGAA GCTGTTCAAG GTCGATGAGA CCGCTCGCAG TGACCTTACC 
CCCAAGGATG TCATTCCTGC AGTTAAGGCC ATGCTTGATC GCATGATCGT TGTCAGAGGA 
GACGATCCTA TCTCCAAGGA GGCAGACTAC AACGCCACGA TCCTGTTCAA GGCCCAGCTT 
CGGTCTCGCT TGGCGTTCAA GCGTCTTGCT GTCCAAGATA GGATCAACAA ATTGGCCTTC 
AACCACATCC TCGGCGAGCT CGAGAACCGT TGGTCGAGGT CGATGGTTTC TCCGGGTGAA 
ATGGTTGGTG TGTTGGCTGC CCAGTCTATC GGTGAACCCG CCACCCAGAT GACACTCAAC 
ACTTTCCATT TCGCCGGTGT GTCCTCCAAG AACGTCACCC TTGGTGTGCC GCGTCTCAAG 
GAAATTCTTA ACGTTGCTTC GAACATCAAG ACTCCTTCCA TGATGGTCTA TCTCGACTCC 
AAGGCAGCGA CCCAGGAGGA GGCCAAGAAG ATGCGCAGTG CTGTCGAGCA CACGAGTCTT 
CGCTCCGTTA CGGCTGTCAC TGAAATCTAC TATGATCCCG ATATCACATC GACCAACATC 
CCCGAGGATT ACGATTTGGT CGAGTCTTAC TTCCTCATTC CCGATCAGAG CGGCGACGAG 
CCAGATCCGA TTGAGAACCA GTCCCGTTGG CTTCTGAGAA TCACTCTCGA TCGTCAGAAG 
ATGCTCGATA AGGGTCTCCG CGTTGAGGAC GTTGCTCACC GCATCAAGGA GGTTTACAAG 
AAGGATGTTG CGGTTGTCTT CTCCGACAAC AACGCTGAAG AGATGGTCAT CCGTATCCGT 
GTTATCAAGG CGGACGATGA CAAGGACGAA GATGGTAATA AGATCATCGA GGACGATGTG 
ATGCTCAAGC GTCTTGAGAA GCATCTCCTG GACGGCTGCA CACTTCGTGG TGTCGAGGGC 
ATTGAGCGTG CCTTCTTGAA CAAGGGTGTT AAGCTGATCG AGGCCAAGGA TGGTTCCATG 
AAGCACTCCA AGGTGGACGA TGAGTGCCAA GAGTGGTACC TTGATACACA AGGTACCTCT 
CTTCGTGAGG TCTTGACTGT CGAGGGTGTT GATACCAAGC GCACCACTAC CAACGACTTG 
TGGCAGGTTG TCGATGTCTT CGGTATCGAG GGAGCTCGTG CTGCTCTGAT GCACGAGTTG 
ACGGCTGTGC TGGCTTTCGA CGGTTCGTAC GTCAACCATC GTCACTTGGC GCTTCTTTGC 
GATGTGATGA CATACAGGGG CAGTATCGCC GCCGTCACAC GTCACGGTAT CAACCGCGCT 
GATACTGGTG CGCTCATGCG TTGCTCTTTC GAGGAGACTG TCGAAATTCT CCTGGAAGCT 
GCCGCCGTCG GTGAGCTTGA TGACTGCCGT GGTATCTCGG AGAACGTTAT GTTAGGCCAG 
ATGGCTCCCA TGGGTACTGG TGCCTTTGAC GTTTTCCTCG ATCCCAAGAT GTTGGAGACC 
GTCATCTCTG ACAACTCTCG CATGGGTCTC ATGCCGGGCA TGACCGTGAA GGGCAGTCAG 
CTGGATGGCG CGGCTACGCC TTATGATACA GGCTCCCCGA TGGCCGACAG TGGGTTCCTT 
GGAAGTTATT CGCCTACCAT GGGCAACTTC TCTCCTATCC AGGGCGCTGG ATCGGACAGC 
CCGACGAACA ACGGCTTTGG CACCGACTAC GGTGGCGTTG GCGGATACGG TTCTGGTACT 
TCCTACTCTT CGGCTACTAG CCCTCGTGCC ACCAGCCCCT TCACACCCAC GTCACCGTTC 
AGCTATGGCG GGTACTCGCC ATCGTCGCCC GCGGCTGGCT ACTCGCCCAG CTCACCTCTC 
CTCGACTCTG CTGGTCGTTT TGCTTCCAGC CCACAATTCA GTCCCTCGTC GCCGTCGTTC 
TCGCCAACTT CGCCGATGCT GCGCCCTGGA AGCCCGACCA GCCCGAGCTA CAGCCCTACC 
TCCCCGAGTT ACTCGCCGGC ATCGCCAGCT GCCGCCAGGT ACTCGCCCAC CTCCCCCGCC 
CAACAATTCT CTCCGACGTC GCCCTCATAC TCCCCCACGA GTCCGAGCTA CAGCCCGGCC 
TCGCCCGCTT TCCAGGCTAC CTCGCCAAGC TACTCTCCGG CGTCGCCTAC CTGGTCGCCA 
ACCTCGCCGG ACGCTTACTC GCCCACGAGC CCGTCGTTCC AGCGGTCGCC TGGTCAGCAG 
CTGTCCCCTA CTAGCCCAAC GGGTTACTCG CCGACGTCGC CGCAGTACTC GCCGCGGACG 
CCGGGACGTA ATAACTCGGG GGGCAACGGG GATCAATATT CTCCCACTTC GCCATCGAAT 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964097.2
CDS	583..5868
Translation	MANLYFAHSSAPLRTIKEIQFGLLSPEEIKGMSVAHIVYPETMDETRTKPRDGGLNDPLLGSVDRQFKCKTCGENMSECPGHFGHIELARPVYHPGFIKRVKKMLEIVCHNCSKVLADRNDKEFAAAMRIRDPKVRFNRVWAVCKKKRRCENEVVKKSDDDEFNPLADAKGELPGHGGCGNDHPTVRQQALTLYAQFDTKDEEGVKSKEKKIITPEMALNILHNMTESDMIDIGINISQARPEWMIITVLPVPPPPVRPSISMDGTGTGMRNEDDLTYKLGDIIRANGNVRQAIQEGSPAHIINDFENLLQYHVATYMDNDIAGQPQALQKSGRPVKAIRARLKGKEGRLRGNLMGKRVDFSARTVITGDANISLDEVGVPRSIARTLTYPETVTPYNIGKLTQYVQNGPNEHPGAKYVIRSDGSRVDLRHQRRTGPLQLEYGWKVERHLIDGDYIIFNRQPSLHKESMMGHRVKVMPYSTFRLNLSVTSPYNADFDGDEMNLHVPQTEETRAEVKELCMVPLNIVSPQRNGPLMGIVQDTLAGAYKLCRRDVFLTKEEVMNIMLWVPEWDGIIPVPAILKPRPRWTGKQIISMVVPKIINLKNIDEDDGECPLKDKGILIQQGEVIFGLMTKKIVGAASGGIVHLCYNELGPSGAMDFLNGCQRVVNYWLLHNGHSIGIGDTIPDAATIAKVQTHIDDAKAEVKQLTDMATNNQLEPLPGMNIRETFENKVSKALNTARDKAGTTTESSLKDINNAVTMALSGSKGSSINISQMTALVGQQIVEGKRIPFGFKYRTLPHFTKDDYSPEARGFVENSYLRGLTPSEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDSAAQYDGTVRNSLGDIIQFVYGEDGLDGIAIENQPVDHFTISNKAFDKRYRLDVMDEEVSSGELEALEYGKELASDPTVQELLDQEYEQLSVDRHMLREIQRTKGNNDTKMQLPLNIIRIIDTAKKLFKVDETARSDLTPKDVIPAVKAMLDRMIVVRGDDPISKEADYNATILFKAQLRSRLAFKRLAVQDRINKLAFNHILGELENRWSRSMVSPGEMVGVLAAQSIGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEILNVASNIKTPSMMVYLDSKAATQEEAKKMRSAVEHTSLRSVTAVTEIYYDPDITSTNIPEDYDLVESYFLIPDQSGDEPDPIENQSRWLLRITLDRQKMLDKGLRVEDVAHRIKEVYKKDVAVVFSDNNAEEMVIRIRVIKADDDKDEDGNKIIEDDVMLKRLEKHLLDGCTLRGVEGIERAFLNKGVKLIEAKDGSMKHSKVDDECQEWYLDTQGTSLREVLTVEGVDTKRTTTNDLWQVVDVFGIEGARAALMHELTAVLAFDGSYVNHRHLALLCDVMTYRGSIAAVTRHGINRADTGALMRCSFEETVEILLEAAAVGELDDCRGISENVMLGQMAPMGTGAFDVFLDPKMLETVISDNSRMGLMPGMTVKGSQLDGAATPYDTGSPMADSGFLGSYSPTMGNFSPIQGAGSDSPTNNGFGTDYGGVGGYGSGTSYSSATSPRATSPFTPTSPFSYGGYSPSSPAAGYSPSSPLLDSAGRFASSPQFSPSSPSFSPTSPMLRPGSPTSPSYSPTSPSYSPASPAAARYSPTSPAQQFSPTSPSYSPTSPSYSPASPAFQATSPSYSPASPTWSPTSPDAYSPTSPSFQRSPGQQLSPTSPTGYSPTSPQYSPRTPGRNNSGGNGDQYSPTSPSND

Target ORF information:

RefSeq Version	XM_959004.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA-directed RNA polymerase II largest subunit (NCU02103), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959004.2

ORF Insert Sequence:

ATGGCGAACC TATACTTTGC GCACTCGAGT GCGCCCTTGC GGACCATCAA GGAAATCCAG 
TTTGGTCTGC TGTCGCCCGA GGAGATCAAG GGCATGAGTG TGGCCCACAT CGTCTACCCA 
GAGACCATGG ACGAGACCAG GACAAAACCC CGCGACGGCG GTCTCAACGA CCCTCTCCTG 
GGCTCCGTAG ATCGTCAGTT CAAGTGCAAG ACGTGCGGCG AGAACATGTC CGAGTGCCCC 
GGCCATTTCG GTCACATCGA GCTGGCCCGA CCCGTCTACC ATCCGGGCTT CATCAAGCGG 
GTGAAGAAGA TGTTGGAGAT TGTCTGCCAC AACTGCAGCA AGGTTCTTGC TGACAGAAAT 
GACAAAGAGT TCGCGGCCGC CATGAGGATC CGCGATCCCA AGGTCCGCTT CAACCGAGTA 
TGGGCTGTGT GCAAGAAAAA GAGGAGATGT GAAAACGAGG TAGTCAAGAA GAGCGACGAT 
GACGAGTTCA ACCCTCTTGC CGACGCCAAG GGCGAGCTCC CCGGCCACGG CGGCTGCGGC 
AACGATCACC CCACCGTCCG CCAGCAGGCC CTCACCCTCT ATGCCCAGTT CGATACCAAG 
GACGAGGAGG GCGTCAAGAG CAAGGAGAAG AAGATCATCA CCCCCGAGAT GGCACTCAAC 
ATTTTGCACA ACATGACCGA GTCTGACATG ATCGACATCG GCATCAACAT CTCTCAAGCT 
CGGCCTGAGT GGATGATCAT CACTGTTCTG CCTGTTCCGC CCCCTCCAGT CCGCCCCAGT 
ATTTCCATGG ACGGTACCGG CACCGGTATG CGGAACGAAG ATGATTTGAC CTACAAGCTC 
GGCGATATCA TTCGAGCAAA CGGCAACGTT CGCCAGGCCA TCCAGGAGGG TTCTCCGGCT 
CACATTATCA ACGATTTCGA GAACCTCCTC CAGTACCACG TTGCTACCTA CATGGACAAC 
GATATTGCTG GTCAACCACA AGCTCTGCAA AAGAGCGGTC GTCCCGTCAA GGCCATTCGT 
GCCCGTCTCA AGGGCAAGGA GGGTCGTCTG CGTGGTAACT TGATGGGCAA GCGTGTCGAT 
TTCTCTGCCC GTACAGTCAT CACAGGCGAT GCCAACATCT CGCTGGATGA AGTCGGTGTG 
CCCCGTAGCA TCGCGCGTAC GCTTACCTAT CCCGAGACTG TGACGCCCTA CAATATTGGC 
AAACTCACCC AATACGTCCA GAACGGCCCT AACGAGCATC CGGGTGCCAA GTACGTTATC 
CGCTCAGATG GCAGCCGTGT CGATCTACGC CATCAGAGGC GGACAGGTCC GCTACAGCTG 
GAATATGGCT GGAAGGTTGA GCGCCATCTC ATCGACGGTG ATTACATCAT CTTCAACCGT 
CAGCCCTCTT TGCACAAGGA ATCCATGATG GGTCATCGTG TCAAGGTCAT GCCCTACTCG 
ACCTTCAGAC TTAACCTTTC TGTCACATCT CCTTACAACG CCGATTTCGA CGGTGATGAA 
ATGAACCTTC ACGTTCCTCA AACCGAAGAA ACTCGTGCTG AAGTCAAGGA GCTTTGCATG 
GTTCCTCTCA ACATCGTCTC GCCGCAGCGT AACGGTCCGC TCATGGGTAT CGTGCAGGAC 
ACTTTGGCTG GTGCTTACAA GCTTTGTCGT CGTGACGTCT TCCTTACCAA GGAGGAGGTC 
ATGAACATTA TGCTCTGGGT CCCCGAGTGG GACGGCATCA TTCCTGTCCC TGCAATCCTC 
AAGCCTCGTC CTCGCTGGAC TGGTAAGCAA ATCATTAGCA TGGTTGTTCC CAAGATCATA 
AACCTCAAGA ACATCGACGA GGATGATGGT GAGTGCCCTC TTAAGGACAA GGGCATTTTG 
ATCCAACAAG GTGAGGTGAT TTTCGGCTTG ATGACGAAGA AGATTGTGGG TGCTGCTAGT 
GGCGGTATCG TCCATCTCTG CTACAACGAG CTGGGTCCTA GCGGTGCTAT GGATTTCCTC 
AACGGCTGCC AGAGAGTCGT CAACTACTGG CTCCTTCACA ATGGTCACAG CATTGGTATT 
GGTGACACTA TTCCCGACGC CGCGACCATC GCCAAGGTCC AGACGCACAT TGACGATGCC 
AAGGCAGAAG TCAAGCAGCT CACAGATATG GCCACCAACA ATCAGCTTGA GCCATTGCCC 
GGTATGAACA TCCGTGAGAC CTTCGAAAAC AAGGTCTCCA AGGCTCTCAA CACGGCCCGT 
GACAAGGCCG GTACTACCAC TGAGAGCAGT TTGAAGGATA TCAACAACGC CGTAACCATG 
GCTCTGTCTG GTTCCAAGGG TTCCTCCATC AACATTTCCC AGATGACTGC CTTGGTCGGT 
CAGCAGATTG TCGAAGGCAA GCGTATTCCT TTCGGCTTCA AGTACCGCAC ACTGCCACAT 
TTCACAAAGG ATGATTACTC GCCCGAAGCC CGTGGTTTCG TCGAGAACTC GTACCTTCGT 
GGTCTTACTC CTTCAGAGTT TTTCTTCCAC GCCATGGCTG GTAGAGAAGG TTTGATCGAT 
ACCGCTGTCA AGACAGCCGA AACTGGTTAC ATTCAGCGTC GTTTGGTCAA GGCCCTTGAA 
GATTCGGCTG CGCAGTACGA TGGTACTGTT CGCAACTCGC TGGGTGATAT CATCCAGTTC 
GTCTACGGTG AAGATGGTCT CGACGGTATT GCCATCGAGA ACCAGCCTGT CGACCACTTC 
ACGATATCCA ACAAGGCTTT CGACAAGCGT TATCGTCTCG ATGTCATGGA CGAGGAGGTT 
TCCTCGGGCG AGCTTGAGGC GCTGGAATAC GGAAAGGAGC TTGCTAGTGA CCCCACTGTT 
CAGGAGCTTC TTGACCAGGA GTACGAACAG CTTTCGGTCG ATCGCCACAT GCTTCGCGAG 
ATCCAGCGCA CAAAAGGCAA CAATGACACG AAGATGCAGC TCCCGCTTAA CATCATTCGT 
ATTATTGATA CTGCCAAGAA GCTGTTCAAG GTCGATGAGA CCGCTCGCAG TGACCTTACC 
CCCAAGGATG TCATTCCTGC AGTTAAGGCC ATGCTTGATC GCATGATCGT TGTCAGAGGA 
GACGATCCTA TCTCCAAGGA GGCAGACTAC AACGCCACGA TCCTGTTCAA GGCCCAGCTT 
CGGTCTCGCT TGGCGTTCAA GCGTCTTGCT GTCCAAGATA GGATCAACAA ATTGGCCTTC 
AACCACATCC TCGGCGAGCT CGAGAACCGT TGGTCGAGGT CGATGGTTTC TCCGGGTGAA 
ATGGTTGGTG TGTTGGCTGC CCAGTCTATC GGTGAACCCG CCACCCAGAT GACACTCAAC 
ACTTTCCATT TCGCCGGTGT GTCCTCCAAG AACGTCACCC TTGGTGTGCC GCGTCTCAAG 
GAAATTCTTA ACGTTGCTTC GAACATCAAG ACTCCTTCCA TGATGGTCTA TCTCGACTCC 
AAGGCAGCGA CCCAGGAGGA GGCCAAGAAG ATGCGCAGTG CTGTCGAGCA CACGAGTCTT 
CGCTCCGTTA CGGCTGTCAC TGAAATCTAC TATGATCCCG ATATCACATC GACCAACATC 
CCCGAGGATT ACGATTTGGT CGAGTCTTAC TTCCTCATTC CCGATCAGAG CGGCGACGAG 
CCAGATCCGA TTGAGAACCA GTCCCGTTGG CTTCTGAGAA TCACTCTCGA TCGTCAGAAG 
ATGCTCGATA AGGGTCTCCG CGTTGAGGAC GTTGCTCACC GCATCAAGGA GGTTTACAAG 
AAGGATGTTG CGGTTGTCTT CTCCGACAAC AACGCTGAAG AGATGGTCAT CCGTATCCGT 
GTTATCAAGG CGGACGATGA CAAGGACGAA GATGGTAATA AGATCATCGA GGACGATGTG 
ATGCTCAAGC GTCTTGAGAA GCATCTCCTG GACGGCTGCA CACTTCGTGG TGTCGAGGGC 
ATTGAGCGTG CCTTCTTGAA CAAGGGTGTT AAGCTGATCG AGGCCAAGGA TGGTTCCATG 
AAGCACTCCA AGGTGGACGA TGAGTGCCAA GAGTGGTACC TTGATACACA AGGTACCTCT 
CTTCGTGAGG TCTTGACTGT CGAGGGTGTT GATACCAAGC GCACCACTAC CAACGACTTG 
TGGCAGGTTG TCGATGTCTT CGGTATCGAG GGAGCTCGTG CTGCTCTGAT GCACGAGTTG 
ACGGCTGTGC TGGCTTTCGA CGGTTCGTAC GTCAACCATC GTCACTTGGC GCTTCTTTGC 
GATGTGATGA CATACAGGGG CAGTATCGCC GCCGTCACAC GTCACGGTAT CAACCGCGCT 
GATACTGGTG CGCTCATGCG TTGCTCTTTC GAGGAGACTG TCGAAATTCT CCTGGAAGCT 
GCCGCCGTCG GTGAGCTTGA TGACTGCCGT GGTATCTCGG AGAACGTTAT GTTAGGCCAG 
ATGGCTCCCA TGGGTACTGG TGCCTTTGAC GTTTTCCTCG ATCCCAAGAT GTTGGAGACC 
GTCATCTCTG ACAACTCTCG CATGGGTCTC ATGCCGGGCA TGACCGTGAA GGGCAGTCAG 
CTGGATGGCG CGGCTACGCC TTATGATACA GGCTCCCCGA TGGCCGACAG TGGGTTCCTT 
GGAAGTTATT CGCCTACCAT GGGCAACTTC TCTCCTATCC AGGGCGCTGG ATCGGACAGC 
CCGACGAACA ACGGCTTTGG CACCGACTAC GGTGGCGTTG GCGGATACGG TTCTGGTACT 
TCCTACTCTT CGGCTACTAG CCCTCGTGCC ACCAGCCCCT TCACACCCAC GTCACCGTTC 
AGCTATGGCG GGTACTCGCC ATCGTCGCCC GCGGCTGGCT ACTCGCCCAG CTCACCTCTC 
CTCGACTCTG CTGGTCGTTT TGCTTCCAGC CCACAATTCA GTCCCTCGTC GCCGTCGTTC 
TCGCCAACTT CGCCGATGCT GCGCCCTGGA AGCCCGACCA GCCCGAGCTA CAGCCCTACC 
TCCCCGAGTT ACTCGCCGGC ATCGCCAGCT GCCGCCAGGT ACTCGCCCAC CTCCCCCGCC 
CAACAATTCT CTCCGACGTC GCCCTCATAC TCCCCCACGA GTCCGAGCTA CAGCCCGGCC 
TCGCCCGCTT TCCAGGCTAC CTCGCCAAGC TACTCTCCGG CGTCGCCTAC CTGGTCGCCA 
ACCTCGCCGG ACGCTTACTC GCCCACGAGC CCGTCGTTCC AGCGGTCGCC TGGTCAGCAG 
CTGTCCCCTA CTAGCCCAAC GGGTTACTCG CCGACGTCGC CGCAGTACTC GCCGCGGACG 
CCGGGACGTA ATAACTCGGG GGGCAACGGG GATCAATATT CTCCCACTTC GCCATCGAAT 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02103 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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