RpII215 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RpII215
Entrez Gene ID	32100
Full Name	RNA polymerase II 215kD subunit
Synonyms	5,8WG16,CG1554,CTD,Dmel\CG1554,H5,II,IIo,L5,POL,Pol II,Pol II CTD,Pol II Ser5P,Pol II Ser5p,Pol II0[ser2],Pol II0[ser5],Pol II[ser2],Pol IIa,Pol IIo,Pol IIo[Ser2],Pol IIo[ser2],Pol IIo[ser5],Pol-II,Pol-IIa,PolII,PolIIa,PolIIo,PolIIo[ser2],PolIIo[ser5],Poll II,RNA Pol II,RNA Pol II CTD,RNA PolI 215,RNA PolII,RNA pol II,RNA pol IIo,RNA polII,RNA-PolII,RNAP,RNAP II,RNAP II LS,RNAPII,RNAPII0,RNApol,RNApol2,RNApolII,RPB1,RPII215,RpII,Rpb1,Rpll215,Ser5-P Pol II,Ubl,alphaPol IIo[ser2],dRPB1,dRpb1,l(1)10Ca,l(1)DC912,l(1)DF912,l(1)G0040,l(1)L5,pol II,polII,rpII1
General protein information	Preferred Names RNA polymerase II 215kD subunit Names CG1554 gene product from transcript CG1554-RA CG1554-PA Pol II CTD Pol II large subunit Pol II large subunit CTD RNA Pol II RNA polymerase II RNA polymerase II large subunit RNA polymerase IIo RNA polymeraseII-215 kd subunit RNA-polymerase II RpII215-PA Ultrabithorax-like polymerase II CTD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10C6-10C6\|1-35.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078569.3	NP_511124.1	RNA polymerase II 215kD subunit

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Homo sapiens (human)	POLR2A	NP_000928.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylation of the C-terminal domain of RNA polymerase II (CTD) plays an essential role in eukaryotic transcription by recruiting transcriptional regulatory factors to the active polymerase. This study shows that Tyrosine 1 is required for CTD phosphorylation by Erk2.
Title: Mapping the Phosphorylation Pattern of Drosophila melanogaster RNA Polymerase II Carboxyl-Terminal Domain Using Ultraviolet Photodissociation Mass Spectrometry.

Genetic and biochemical analyses reveal a functional interaction of MSL1 with CDK7, a subunit of the Cdk-activating kinase (CAK) complex of the general transcription factor TFIIH. Importantly, MSL1 depletion leads to decreased phosphorylation of Ser5 of RNA polymerase II.
Title: Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation.

This action of GAF on nucleosomes is at least partially independent of paused Pol II because intergenic GAF binding sites with little or no Pol II also show GAF-dependent nucleosome displacement.
Title: GAGA factor maintains nucleosome-free regions and has a role in RNA polymerase II recruitment to promoters.

Study provides additional evidence that Zeste protein plays no role in the activation of the white gene transcription; does not rule out that the white gene enhancer is able to recruit RNAPII and function as a weak promoter
Title: Functional organization of the white gene enhancer in Drosophila melanogaster.

Loss of dPNUTS function or specific disruption of its ability to bind PP1 results in hyperphosphorylation of the RNAPII CTD in whole animal extracts and on chromosomes
Title: PNUTS/PP1 regulates RNAPII-mediated gene expression and is necessary for developmental growth.

The following RpII215 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpII215 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01288	NM_078569.3 Latest version!	Drosophila melanogaster RNA polymerase II 215kD subunit (RpII215), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RpII215 ( NM_078569.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511124.1 Drosophila melanogaster RNA polymerase II 215kD subunit (RpII215), mRNA.

Price & Availability↑

CloneID	OFa01288
Clone ID Related Accession (Same CDS sequence)	NM_078569.3
Accession Version	NM_078569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RNA polymerase II 215kD subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078569.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACCC CCACGGACTC GAAGGCGCCG TTGCGCCAGG TGAAGCGCGT ACAGTTCGGC 
ATTTTGTCCC CTGATGAAAT TCGTCGCATG TCCGTCACCG AGGGTGGCGT CCAGTTTGCG 
GAGACAATGG AGGGCGGACG ACCAAAGCTG GGCGGATTGA TGGATCCGCG CCAGGGTGTC 
ATAGACAGGA CGTCGCGCTG TCAGACGTGC GCCGGCAATA TGACCGAGTG CCCCGGTCAC 
TTTGGACACA TCGACCTGGC CAAACCAGTG TTCCACATCG GCTTCATCAC CAAGACAATT 
AAGATTTTGC GATGTGTGTG CTTCTACTGC TCCAAAATGT TGGTCTCGCC ACACAATCCA 
AAGATCAAGG AGATCGTTAT GAAGTCTAGG GGACAGCCGC GTAAGCGCCT GGCCTACGTC 
TATGATCTGT GCAAGGGCAA GACGATTTGC GAGGGCGGCG AGGACATGGA TCTCACCAAG 
GAGAACCAGC AGCCGGATCC AAACAAAAAG CCCGGCCACG GCGGTTGCGG TCACTACCAG 
CCGTCGATCC GGAGAACAGG ACTGGATCTC ACCGCCGAGT GGAAGCATCA GAACGAGGAT 
TCGCAGGAGA AGAAGATCGT GGTCTCGGCA GAGCGGGTTT GGGAAATCCT TAAGCATATC 
ACCGATGAGG AGTGCTTTAT TCTGGGCATG GATCCCAAGT ATGCGCGTCC CGATTGGATG 
ATCGTGACCG TCCTGCCTGT TCCACCGTTG GCCGTACGGC CAGCGGTCGT GATGTTTGGT 
GCTGCCAAGA ATCAGGATGA TTTGACGCAC AAGCTGTCCG ATATCATCAA GGCAAACAAT 
GAGCTTCGCA AGAACGAGGC CAGTGGTGCC GCAGCGCATG TGATCCAGGA GAACATCAAG 
ATGCTGCAAT TCCACGTCGC CACACTAGTG GACAACGATA TGCCCGGCAT GCCCAGGGCT 
ATGCAAAAGT CGGGAAAACC CCTTAAAGCC ATCAAGGCGC GACTCAAGGG TAAGGAGGGT 
AGGATTCGTG GCAACTTGAT GGGCAAACGT GTCGACTTCT CCGCCCGTAC TGTCATCACA 
CCCGATCCAA ATCTGCGTAT CGATCAGGTC GGTGTGCCGC GTTCCATTGC CCAGAATCTA 
ACCTTCCCCG AGCTGGTCAC TCCCTTCAAC ATCGATCGCA TGCAGGAGCT GGTGCGCAGG 
GGTAATTCGC AGTATCCGGG TGCCAAGTAC ATTGTGCGCG ACAATGGCGA GCGTATTGAT 
CTTCGCTTTC ATCCTAAATC CTCTGATTTA CATCTGCAGT GCGGCTACAA GGTAGAGCGG 
CATTTGCGCG ACGACGATCT GGTGATCTTC AACCGACAGC CCACGCTGCA CAAGATGAGT 
ATGATGGGTC ACAGGGTGAA AGTGTTGCCC TGGTCGACTT TCCGCATGAA CCTGTCGTGT 
ACATCGCCCT ACAATGCTGA TTTCGACGGC GATGAAATGA ATTTGCACGT TCCGCAGTCC 
ATGGAGACGC GCGCCGAGGT GGAGAACATC CACATCACAC CCAGACAGAT CATCACGCCG 
CAGGCGAACA AACCTGTCAT GGGTATCGTG CAGGACACTC TGACTGCGGT GCGAAAGATG 
ACCAAGCGCG ACGTATTCAT CACGCGCGAG CAGGTGATGA ATCTGCTCAT GTTCCTGCCC 
ACGTGGGACG CTAAAATGCC GCAACCCTGC ATCCTGAAAC CGCGTCCCCT GTGGACGGGC 
AAACAGATCT TCTCCCTGAT CATCCCCGGC AACGTGAACA TGATACGCAC ACATTCCACG 
CATCCGGACG AGGAGGATGA AGGACCATAC AAGTGGATCT CGCCCGGTGA CACCAAGGTA 
ATGGTGGAGC ACGGTGAACT TATCATGGGG ATCCTCTGCA AGAAGTCACT GGGTACATCA 
GCCGGATCAC TGCTGCATAT TTGTTTCCTG GAACTTGGTC ACGATATCGC TGGTCGGTTC 
TATGGCAACA TTCAGACCGT GATCAACAAT TGGTTGCTTT TCGAGGGCCA TAGTATCGGT 
ATTGGTGACA CTATTGCCGA TCCCCAGACC TACAACGAGA TCCAGCAGGC CATCAAGAAG 
GCAAAGGACG ACGTGATAAA CGTTATCCAA AAGGCCCACA ACATGGAGCT GGAGCCCACG 
CCGGGTAATA CTCTGCGTCA GACGTTCGAG AACAAGGTGA ACCGTATCCT AAACGATGCT 
CGTGACAAGA CTGGTGGTTC GGCCAAGAAA TCTCTCACTG AGTACAACAA TCTAAAGGCT 
ATGGTGGTGT CTGGTTCCAA GGGTTCCAAC ATTAATATAT CCCAGGTTAT TGCTTGTGTG 
GGTCAACAGA ACGTGGAGGG TAAGCGAATC CCCTACGGTT TCCGCAAACG CACTCTTCCC 
CACTTTATTA AGGACGATTA TGGTCCTGAG TCGCGCGGTT TCGTGGAGAA TTCGTATCTT 
GCCGGCCTGA CACCCTCTGA GTTCTATTTC CACGCTATGG GTGGTCGTGA AGGTCTTATC 
GATACTGCTG TAAAGACAGC TGAAACCGGT TACATCCAGC GTCGTCTTAT AAAGGCTATG 
GAGTCGGTGA TGGTTAACTA CGACGGAACA GTGCGTAACT CGGTGGGCCA GCTTATTCAG 
CTGCGTTACG GCGAGGACGG TCTTTGCGGC GAGCTGGTTG AGTTCCAGAA CATGCCAACG 
GTGAAACTGT CGAACAAGTC GTTTGAAAAG CGCTTCAAAT TTGACTGGAG CAACGAGCGA 
TTAATGAAAA AGGTATTTAC GGATGATGTG ATCAAGGAGA TGACCGACAG CAGCGAAGCC 
ATCCAGGAAC TGGAGGCAGA GTGGGATCGT TTGGTTTCCG ATCGCGACAG TTTGAGACAA 
ATCTTCCCTA ACGGCGAATC CAAGGTGGTA CTGCCGTGCA ACCTGCAGCG TATGATCTGG 
AATGTGCAGA AGATCTTTCA CATCAACAAG CGCCTGCCCA CGGATCTCTC GCCCATTCGG 
GTGATCAAGG GCGTTAAGAC ATTGCTCGAA CGCTGTGTTA TTGTCACGGG AAATGATCGA 
ATTTCAAAGC AGGCCAACGA GAACGCCACG TTGCTATTCC AGTGCCTAAT CCGTTCGACA 
CTATGTACGA AATACGTGTC GGAGGAGTTC CGCCTGTCCA CAGAGGCGTT TGAGTGGTTG 
GTCGGAGAAA TCGAGACGCG TTTCCAACAG GCCCAGGCCA ATCCCGGCGA GATGGTGGGT 
GCCCTGGCTG CCCAGAGTTT GGGTGAGCCC GCTACTCAGA TGACACTGAA CACCTTCCAT 
TTTGCTGGTG TGTCTTCAAA GAACGTAACA TTGGGTGTGC CGCGTCTCAA GGAGATTATC 
AACATATCCA AAAAGCCCAA AGCGCCGTCG CTAACCGTTT TCCTTACTGG CGGCGCTGCT 
CGCGATGCGG AGAAGGCCAA GAACGTACTG TGCCGCCTGG AGCATACCAC GCTGCGCAAG 
GTTACGGCCA ATACGGCTAT TTACTACGAC CCGGATCCGC AGAGAACGGT GATATCCGAG 
GATCAAGAAT TTGTGAATGT CTACTACGAA ATGCCCGACT TTGATCCCAC ACGCATTTCG 
CCCTGGTTGC TACGTATTGA ATTGGACCGC AAGCGGATGA CGGACAAGAA GCTGACAATG 
GAACAGATCG CCGAGAAGAT TAACGTGGGC TTCGGGGAGG ATCTCAATTG TATTTTCAAT 
GACGACAATG CCGATAAGCT GGTGCTGCGC ATTAGGATCA TGAACAACGA AGAGAACAAG 
TTCCAGGACG AAGACGAGGC CGTCGACAAG ATGGAGGACG ACATGTTCTT GCGCTGCATT 
GAGGCCAACA TGCTGTCGGA CATGACACTG CAGGGTATCG AAGCCATCGG CAAGGTGTAC 
ATGCATCTGC CACAGACCGA CAGCAAGAAG CGTATCGTGA TCACTGAGAC CGGAGAGTTT 
AAGGCCATCG GCGAGTGGCT GCTCGAGACG GACGGCACAT CGATGATGAA AGTGCTGTCT 
GAGCGTGACG TGGACCCAAT CCGAACATCC TCCAACGATA TTTGCGAGAT ATTCCAGGTG 
CTGGGCATCG AGGCGGTGCG AAAGTCTGTC GAAAAGGAGA TGAACGCCGT GTTGCAGTTC 
TACGGCCTGT ACGTGAACTA TCGTCACTTG GCCCTGTTGT GCGATGTGAT GACGGCCAAG 
GGCCATCTGA TGGCCATCAC CCGTCACGGC ATTAACAGGC AAGACACTGG TGCCCTTATG 
CGTTGCTCCT TTGAGGAGAC AGTGGATGTG CTAATGGATG CCGCCGCTCA CGCCGAAACG 
GATCCCATGA GGGGCGTCTC TGAGAACATT ATCATGGGAC AGCTGCCCAA AATGGGTACC 
GGCTGCTTTG ACCTTCTGCT CGATGCAGAG AAGTGTCGTT TCGGCATCGA GATTCCCAAT 
ACGTTGGGCA ACAGTATGCT AGGTGGCGCC GCTATGTTCA TTGGCGGTGG ATCGACACCG 
AGCATGACGC CACCGATGAC GCCGTGGGCT AACTGCAACA CGCCGCGATA CTTCTCTCCA 
CCCGGCCACG TAAGTGCCAT GACTCCTGGC GGTCCCAGTT TCTCGCCTTC GGCTGCATCG 
GATGCGTCCG GAATGTCGCC TAGCTGGTCG CCGGCTCATC CGGGCTCATC GCCCAGTTCA 
CCAGGACCTT CGATGTCGCC GTATTTCCCA GCCTCGCCGA GTGTTTCTCC CTCTTATTCG 
CCAACGAGTC CGAACTACAC GGCATCTTCT CCCGGTGGAG CCTCGCCGAA TTACTCGCCC 
TCGAGTCCGA ACTATTCGCC GACGTCGCCG CTTTATGCAA GTCCACGTTA CGCATCGACA 
ACGCCAAATT TCAATCCACA GTCGACGGGT TACTCGCCAT CTTCATCGGG ATACTCGCCA 
ACATCCCCGG TCTACTCGCC CACGGTGCAA TTCCAGTCGA GTCCGTCGTT TGCGGGCAGC 
GGTAGCAACA TTTACTCGCC GGGCAATGCG TACTCGCCCA GCTCGTCCAA CTACTCCCCC 
AATTCACCAT CCTACTCGCC GACATCACCA TCGTACTCGC CGTCAAGTCC TTCGTACTCG 
CCAACGTCGC CTTGCTATTC GCCCACATCG CCTTCGTACT CGCCAACGAG TCCGAACTAC 
ACACCCGTAA CACCCTCATA CTCGCCGACA AGTCCGAACT ATTCAGCGTC GCCGCAATAT 
TCTCCAGCCT CGCCAGCTTA CTCGCAAACG GGGGTGAAGT ACTCACCGAC ATCGCCGACG 
TACTCGCCGC CGTCACCATC GTACGATGGG TCTCCCGGAT CACCACAATA TACGCCAGGA 
TCTCCGCAGT ACTCGCCGGC CTCGCCTAAG TACTCGCCGA CCTCACCGCT GTACTCGCCC 
AGCTCGCCGC AGCACTCGCC CTCAAACCAG TACAGCCCAA CAGGATCGAC CTATTCGGCG 
ACGAGTCCGC GGTACTCGCC GAACATGTCC ATCTACTCGC CGAGCAGCAC CAAGTACTCG 
CCCACCTCGC CAACGTACAC ACCGACGGCC CGCAACTACT CGCCCACGTC ACCGATGTAC 
TCGCCAACGG CTCCATCGCA CTACAGTCCC ACGAGTCCGG CCTACTCGCC CAGCAGTCCC 
ACGTTCGAGG AGAGCGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511124.1
CDS	361..6024
Translation	MSTPTDSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRKRLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDPDPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQIAEKINVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGIEAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIPNTLGNSMLGGAAMFIGGGSTPSMTPPMTPWANCNTPRYFSPPGHVSAMTPGGPSFSPSAASDASGMSPSWSPAHPGSSPSSPGPSMSPYFPASPSVSPSYSPTSPNYTASSPGGASPNYSPSSPNYSPTSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNIYSPGNAYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYSASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSPSSPQHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPMYSPTAPSHYSPTSPAYSPSSPTFEESED

Target ORF information:

RefSeq Version	NM_078569.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RNA polymerase II 215kD subunit (RpII215), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078569.3

ORF Insert Sequence:

ATGAGCACCC CCACGGACTC GAAGGCGCCG TTGCGCCAGG TGAAGCGCGT ACAGTTCGGC 
ATTTTGTCCC CTGATGAAAT TCGTCGCATG TCCGTCACCG AGGGTGGCGT CCAGTTTGCG 
GAGACAATGG AGGGCGGACG ACCAAAGCTG GGCGGATTGA TGGATCCGCG CCAGGGTGTC 
ATAGACAGGA CGTCGCGCTG TCAGACGTGC GCCGGCAATA TGACCGAGTG CCCCGGTCAC 
TTTGGACACA TCGACCTGGC CAAACCAGTG TTCCACATCG GCTTCATCAC CAAGACAATT 
AAGATTTTGC GATGTGTGTG CTTCTACTGC TCCAAAATGT TGGTCTCGCC ACACAATCCA 
AAGATCAAGG AGATCGTTAT GAAGTCTAGG GGACAGCCGC GTAAGCGCCT GGCCTACGTC 
TATGATCTGT GCAAGGGCAA GACGATTTGC GAGGGCGGCG AGGACATGGA TCTCACCAAG 
GAGAACCAGC AGCCGGATCC AAACAAAAAG CCCGGCCACG GCGGTTGCGG TCACTACCAG 
CCGTCGATCC GGAGAACAGG ACTGGATCTC ACCGCCGAGT GGAAGCATCA GAACGAGGAT 
TCGCAGGAGA AGAAGATCGT GGTCTCGGCA GAGCGGGTTT GGGAAATCCT TAAGCATATC 
ACCGATGAGG AGTGCTTTAT TCTGGGCATG GATCCCAAGT ATGCGCGTCC CGATTGGATG 
ATCGTGACCG TCCTGCCTGT TCCACCGTTG GCCGTACGGC CAGCGGTCGT GATGTTTGGT 
GCTGCCAAGA ATCAGGATGA TTTGACGCAC AAGCTGTCCG ATATCATCAA GGCAAACAAT 
GAGCTTCGCA AGAACGAGGC CAGTGGTGCC GCAGCGCATG TGATCCAGGA GAACATCAAG 
ATGCTGCAAT TCCACGTCGC CACACTAGTG GACAACGATA TGCCCGGCAT GCCCAGGGCT 
ATGCAAAAGT CGGGAAAACC CCTTAAAGCC ATCAAGGCGC GACTCAAGGG TAAGGAGGGT 
AGGATTCGTG GCAACTTGAT GGGCAAACGT GTCGACTTCT CCGCCCGTAC TGTCATCACA 
CCCGATCCAA ATCTGCGTAT CGATCAGGTC GGTGTGCCGC GTTCCATTGC CCAGAATCTA 
ACCTTCCCCG AGCTGGTCAC TCCCTTCAAC ATCGATCGCA TGCAGGAGCT GGTGCGCAGG 
GGTAATTCGC AGTATCCGGG TGCCAAGTAC ATTGTGCGCG ACAATGGCGA GCGTATTGAT 
CTTCGCTTTC ATCCTAAATC CTCTGATTTA CATCTGCAGT GCGGCTACAA GGTAGAGCGG 
CATTTGCGCG ACGACGATCT GGTGATCTTC AACCGACAGC CCACGCTGCA CAAGATGAGT 
ATGATGGGTC ACAGGGTGAA AGTGTTGCCC TGGTCGACTT TCCGCATGAA CCTGTCGTGT 
ACATCGCCCT ACAATGCTGA TTTCGACGGC GATGAAATGA ATTTGCACGT TCCGCAGTCC 
ATGGAGACGC GCGCCGAGGT GGAGAACATC CACATCACAC CCAGACAGAT CATCACGCCG 
CAGGCGAACA AACCTGTCAT GGGTATCGTG CAGGACACTC TGACTGCGGT GCGAAAGATG 
ACCAAGCGCG ACGTATTCAT CACGCGCGAG CAGGTGATGA ATCTGCTCAT GTTCCTGCCC 
ACGTGGGACG CTAAAATGCC GCAACCCTGC ATCCTGAAAC CGCGTCCCCT GTGGACGGGC 
AAACAGATCT TCTCCCTGAT CATCCCCGGC AACGTGAACA TGATACGCAC ACATTCCACG 
CATCCGGACG AGGAGGATGA AGGACCATAC AAGTGGATCT CGCCCGGTGA CACCAAGGTA 
ATGGTGGAGC ACGGTGAACT TATCATGGGG ATCCTCTGCA AGAAGTCACT GGGTACATCA 
GCCGGATCAC TGCTGCATAT TTGTTTCCTG GAACTTGGTC ACGATATCGC TGGTCGGTTC 
TATGGCAACA TTCAGACCGT GATCAACAAT TGGTTGCTTT TCGAGGGCCA TAGTATCGGT 
ATTGGTGACA CTATTGCCGA TCCCCAGACC TACAACGAGA TCCAGCAGGC CATCAAGAAG 
GCAAAGGACG ACGTGATAAA CGTTATCCAA AAGGCCCACA ACATGGAGCT GGAGCCCACG 
CCGGGTAATA CTCTGCGTCA GACGTTCGAG AACAAGGTGA ACCGTATCCT AAACGATGCT 
CGTGACAAGA CTGGTGGTTC GGCCAAGAAA TCTCTCACTG AGTACAACAA TCTAAAGGCT 
ATGGTGGTGT CTGGTTCCAA GGGTTCCAAC ATTAATATAT CCCAGGTTAT TGCTTGTGTG 
GGTCAACAGA ACGTGGAGGG TAAGCGAATC CCCTACGGTT TCCGCAAACG CACTCTTCCC 
CACTTTATTA AGGACGATTA TGGTCCTGAG TCGCGCGGTT TCGTGGAGAA TTCGTATCTT 
GCCGGCCTGA CACCCTCTGA GTTCTATTTC CACGCTATGG GTGGTCGTGA AGGTCTTATC 
GATACTGCTG TAAAGACAGC TGAAACCGGT TACATCCAGC GTCGTCTTAT AAAGGCTATG 
GAGTCGGTGA TGGTTAACTA CGACGGAACA GTGCGTAACT CGGTGGGCCA GCTTATTCAG 
CTGCGTTACG GCGAGGACGG TCTTTGCGGC GAGCTGGTTG AGTTCCAGAA CATGCCAACG 
GTGAAACTGT CGAACAAGTC GTTTGAAAAG CGCTTCAAAT TTGACTGGAG CAACGAGCGA 
TTAATGAAAA AGGTATTTAC GGATGATGTG ATCAAGGAGA TGACCGACAG CAGCGAAGCC 
ATCCAGGAAC TGGAGGCAGA GTGGGATCGT TTGGTTTCCG ATCGCGACAG TTTGAGACAA 
ATCTTCCCTA ACGGCGAATC CAAGGTGGTA CTGCCGTGCA ACCTGCAGCG TATGATCTGG 
AATGTGCAGA AGATCTTTCA CATCAACAAG CGCCTGCCCA CGGATCTCTC GCCCATTCGG 
GTGATCAAGG GCGTTAAGAC ATTGCTCGAA CGCTGTGTTA TTGTCACGGG AAATGATCGA 
ATTTCAAAGC AGGCCAACGA GAACGCCACG TTGCTATTCC AGTGCCTAAT CCGTTCGACA 
CTATGTACGA AATACGTGTC GGAGGAGTTC CGCCTGTCCA CAGAGGCGTT TGAGTGGTTG 
GTCGGAGAAA TCGAGACGCG TTTCCAACAG GCCCAGGCCA ATCCCGGCGA GATGGTGGGT 
GCCCTGGCTG CCCAGAGTTT GGGTGAGCCC GCTACTCAGA TGACACTGAA CACCTTCCAT 
TTTGCTGGTG TGTCTTCAAA GAACGTAACA TTGGGTGTGC CGCGTCTCAA GGAGATTATC 
AACATATCCA AAAAGCCCAA AGCGCCGTCG CTAACCGTTT TCCTTACTGG CGGCGCTGCT 
CGCGATGCGG AGAAGGCCAA GAACGTACTG TGCCGCCTGG AGCATACCAC GCTGCGCAAG 
GTTACGGCCA ATACGGCTAT TTACTACGAC CCGGATCCGC AGAGAACGGT GATATCCGAG 
GATCAAGAAT TTGTGAATGT CTACTACGAA ATGCCCGACT TTGATCCCAC ACGCATTTCG 
CCCTGGTTGC TACGTATTGA ATTGGACCGC AAGCGGATGA CGGACAAGAA GCTGACAATG 
GAACAGATCG CCGAGAAGAT TAACGTGGGC TTCGGGGAGG ATCTCAATTG TATTTTCAAT 
GACGACAATG CCGATAAGCT GGTGCTGCGC ATTAGGATCA TGAACAACGA AGAGAACAAG 
TTCCAGGACG AAGACGAGGC CGTCGACAAG ATGGAGGACG ACATGTTCTT GCGCTGCATT 
GAGGCCAACA TGCTGTCGGA CATGACACTG CAGGGTATCG AAGCCATCGG CAAGGTGTAC 
ATGCATCTGC CACAGACCGA CAGCAAGAAG CGTATCGTGA TCACTGAGAC CGGAGAGTTT 
AAGGCCATCG GCGAGTGGCT GCTCGAGACG GACGGCACAT CGATGATGAA AGTGCTGTCT 
GAGCGTGACG TGGACCCAAT CCGAACATCC TCCAACGATA TTTGCGAGAT ATTCCAGGTG 
CTGGGCATCG AGGCGGTGCG AAAGTCTGTC GAAAAGGAGA TGAACGCCGT GTTGCAGTTC 
TACGGCCTGT ACGTGAACTA TCGTCACTTG GCCCTGTTGT GCGATGTGAT GACGGCCAAG 
GGCCATCTGA TGGCCATCAC CCGTCACGGC ATTAACAGGC AAGACACTGG TGCCCTTATG 
CGTTGCTCCT TTGAGGAGAC AGTGGATGTG CTAATGGATG CCGCCGCTCA CGCCGAAACG 
GATCCCATGA GGGGCGTCTC TGAGAACATT ATCATGGGAC AGCTGCCCAA AATGGGTACC 
GGCTGCTTTG ACCTTCTGCT CGATGCAGAG AAGTGTCGTT TCGGCATCGA GATTCCCAAT 
ACGTTGGGCA ACAGTATGCT AGGTGGCGCC GCTATGTTCA TTGGCGGTGG ATCGACACCG 
AGCATGACGC CACCGATGAC GCCGTGGGCT AACTGCAACA CGCCGCGATA CTTCTCTCCA 
CCCGGCCACG TAAGTGCCAT GACTCCTGGC GGTCCCAGTT TCTCGCCTTC GGCTGCATCG 
GATGCGTCCG GAATGTCGCC TAGCTGGTCG CCGGCTCATC CGGGCTCATC GCCCAGTTCA 
CCAGGACCTT CGATGTCGCC GTATTTCCCA GCCTCGCCGA GTGTTTCTCC CTCTTATTCG 
CCAACGAGTC CGAACTACAC GGCATCTTCT CCCGGTGGAG CCTCGCCGAA TTACTCGCCC 
TCGAGTCCGA ACTATTCGCC GACGTCGCCG CTTTATGCAA GTCCACGTTA CGCATCGACA 
ACGCCAAATT TCAATCCACA GTCGACGGGT TACTCGCCAT CTTCATCGGG ATACTCGCCA 
ACATCCCCGG TCTACTCGCC CACGGTGCAA TTCCAGTCGA GTCCGTCGTT TGCGGGCAGC 
GGTAGCAACA TTTACTCGCC GGGCAATGCG TACTCGCCCA GCTCGTCCAA CTACTCCCCC 
AATTCACCAT CCTACTCGCC GACATCACCA TCGTACTCGC CGTCAAGTCC TTCGTACTCG 
CCAACGTCGC CTTGCTATTC GCCCACATCG CCTTCGTACT CGCCAACGAG TCCGAACTAC 
ACACCCGTAA CACCCTCATA CTCGCCGACA AGTCCGAACT ATTCAGCGTC GCCGCAATAT 
TCTCCAGCCT CGCCAGCTTA CTCGCAAACG GGGGTGAAGT ACTCACCGAC ATCGCCGACG 
TACTCGCCGC CGTCACCATC GTACGATGGG TCTCCCGGAT CACCACAATA TACGCCAGGA 
TCTCCGCAGT ACTCGCCGGC CTCGCCTAAG TACTCGCCGA CCTCACCGCT GTACTCGCCC 
AGCTCGCCGC AGCACTCGCC CTCAAACCAG TACAGCCCAA CAGGATCGAC CTATTCGGCG 
ACGAGTCCGC GGTACTCGCC GAACATGTCC ATCTACTCGC CGAGCAGCAC CAAGTACTCG 
CCCACCTCGC CAACGTACAC ACCGACGGCC CGCAACTACT CGCCCACGTC ACCGATGTAC 
TCGCCAACGG CTCCATCGCA CTACAGTCCC ACGAGTCCGG CCTACTCGCC CAGCAGTCCC 
ACGTTCGAGG AGAGCGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RpII215 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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