rpb1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rpb1
Entrez Gene ID	2540292
Full Name	RNA polymerase II large subunit Rpb1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021568.2	NP_595673.1	RNA polymerase II large subunit Rpb1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Homo sapiens (human)	POLR2A	NP_000928.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Seb1 is critical for poly(A) site selection by binding to the C-terminal domain (CTD) of RNAPII via a conserved CTD-interaction domain and recognizes specific sequence motifs clustered downstream of the polyadenylation site on the uncleaved pre-mRNA.
Title: Polyadenylation site selection: linking transcription and RNA processing via a conserved carboxy-terminal domain (CTD)-interacting protein.

Examination of the roles of known Rpb1 phosphorylation sites in co-transcriptional histone modifications.
Title: Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification.

Individual letters of the RNA polymerase II CTD code govern distinct gene expression programs in fission yeast.
Title: Individual letters of the RNA polymerase II CTD code govern distinct gene expression programs in fission yeast.

Separate domains of Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition.
Title: Separate domains of fission yeast Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition.

The following rpb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rpb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101506	NM_001021568.2 Latest version!	Schizosaccharomyces pombe RNA polymerase II large subunit Rpb1 (rpb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rpb1 ( NM_001021568.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595673.1 Schizosaccharomyces pombe RNA polymerase II large subunit Rpb1 (rpb1), mRNA.

Price & Availability↑

CloneID	OSc101506
Clone ID Related Accession (Same CDS sequence)	NM_001021568.2
Accession Version	NM_001021568.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RNA polymerase II large subunit Rpb1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021568.1.

ATGAGCGGGA TTCAGTTTTC ACCTTCTTCT GTCCCCTTAC GCCGGGTGGA GGAGGTTCAA 
TTTGGAATCT TGTCCCCGGA GGAAATTCGT TCAATGAGCG TTGCGAAGAT TGAGTTTCCT 
GAGACCATGG ATGAAAGTGG ACAACGCCCC CGTGTTGGTG GGCTCCTGGA CCCTCGATTG 
GGGACTATTG ATCGACAATT CAAGTGTCAA ACCTGTGGTG AAACTATGGC GGATTGTCCT 
GGTCATTTTG GTCATATTGA ACTTGCAAAG CCAGTTTTCC ACATCGGTTT CCTAAGCAAA 
ATAAAAAAAA TATTAGAATG CGTTTGTTGG AATTGCGGCA AACTTAAGAT TGATTCTTCA 
AATCCAAAAT TTAATGACAC ACAACGTTAT CGTGATCCCA AAAATCGATT AAATGCAGTT 
TGGAATGTCT GCAAGACAAA AATGGTTTGC GATACTGGCT TATCTGCAGG CTCAGATAAT 
TTCGACCTTA GTAATCCCTC CGCCAATATG GGACACGGTG GTTGTGGGGC TGCCCAACCT 
ACAATACGTA AAGATGGCCT GAGATTATGG GGTTCTTGGA AACGCGGTAA AGATGAATCA 
GATTTACCAG AAAAACGCCT TCTGTCGCCG TTGGAGGTGC ACACAATATT CACACATATT 
TCTTCAGAAG ATTTAGCTCA CTTAGGTCTT AATGAGCAGT ATGCTAGACC TGACTGGATG 
ATTATTACAG TCTTACCTGT TCCTCCTCCG AGTGTCCGTC CTAGTATTTC GGTGGATGGA 
ACCAGTCGTG GCGAAGATGA TTTAACGCAC AAACTTTCCG ATATCATAAA AGCAAATGCC 
AACGTTCGAC GGTGTGAACA AGAAGGAGCT CCTGCGCATA TTGTCTCTGA ATATGAACAG 
TTACTTCAGT TCCATGTTGC CACGTATATG GACAATGAAA TAGCGGGACA GCCTCAGGCT 
TTACAAAAAT CTGGCAGACC TCTTAAAAGT ATCCGTGCAC GGCTCAAGGG AAAAGAGGGT 
CGATTACGTG GCAACCTGAT GGGTAAACGG GTTGATTTTT CAGCTCGTAC TGTGATTACC 
GGTGATCCAA ATTTGTCGTT AGATGAACTC GGTGTTCCAC GTAGTATTGC TAAGACACTT 
ACTTATCCTG AAACTGTTAC ACCTTATAAT ATTTATCAAT TGCAAGAATT AGTACGAAAT 
GGTCCTGATG AGCATCCCGG TGCTAAATAT ATCATTCGTG ATACAGGAGA GCGTATTGAC 
TTGCGTTATC ATAAACGTGC TGGTGATATT CCTCTTAGGT ACGGTTGGCG AGTTGAGCGG 
CATATTCGTG ATGGCGATGT CGTTATTTTT AACAGACAGC CATCCCTTCA CAAAATGAGT 
ATGATGGGTC ATAGGATTCG CGTAATGCCG TACTCGACAT TCCGATTAAA CTTGTCAGTT 
ACGTCTCCTT ATAATGCTGA CTTTGATGGT GATGAAATGA ATATGCATGT CCCTCAGTCA 
GAAGAAACTC GTGCAGAAAT TCAGGAAATT ACTATGGTAC CTAAGCAGAT TGTTTCTCCT 
CAATCAAACA AGCCTGTCAT GGGTATTGTT CAAGATACTT TAGCAGGTGT TCGTAAGTTT 
TCGTTACGTG ATAACTTTTT GACTCGCAAC GCTGTAATGA ATATTATGTT ATGGGTACCA 
GACTGGGATG GAATACTCCC ACCACCCGTC ATTCTTAAAC CAAAGGTTTT ATGGACAGGA 
AAGCAAATTT TGAGCTTGAT TATACCCAAG GGAATTAACT TAATTAGAGA CGACGATAAG 
CAAAGTCTTT CAAATCCCAC CGACTCAGGA ATGTTGATTG AAAATGGTGA AATTATATAC 
GGCGTGGTTG ACAAGAAGAC TGTTGGTGCT TCTCAAGGTG GTTTAGTTCA TACTATCTGG 
AAAGAGAAAG GTCCTGAGAT TTGTAAAGGT TTCTTCAATG GTATTCAACG AGTTGTAAAC 
TATTGGCTTT TACATAACGG TTTTAGCATT GGTATAGGAG ATACTATTGC CGATGCGGAC 
ACAATGAAAG AGGTTACTAG AACTGTTAAG GAAGCCCGTC GTCAAGTCGC AGAATGTATT 
CAGGATGCTC AGCACAATCG CTTAAAGCCT GAGCCAGGAA TGACCCTTCG AGAATCTTTT 
GAAGCTAAGG TATCAAGGAT TCTCAATCAA GCGAGAGACA ACGCTGGTAG GTCAGCTGAG 
CATAGCTTGA AGGATTCTAA CAATGTCAAA CAAATGGTTG CTGCTGGTTC AAAAGGTTCA 
TTTATTAATA TCTCTCAAAT GTCTGCTTGC GTCGGTCAGC AAATCGTTGA AGGCAAACGT 
ATTCCTTTTG GATTTAAATA TCGTACATTA CCACATTTTC CTAAAGACGA CGACTCTCCA 
GAATCTCGTG GATTTATTGA AAATTCATAC TTAAGGGGTT TAACTCCTCA AGAGTTCTTT 
TTCCACGCAA TGGCTGGACG TGAGGGTTTG ATTGATACTG CGGTCAAAAC TGCTGAAACT 
GGATACATCC AAAGGCGTCT TGTTAAGGCT ATGGAGGATG TAATGGTTCG CTATGACGGT 
ACTGTGCGCA ATGCCATGGG TGACATTATA CAATTTGCCT ATGGTGAAGA TGGCCTGGAT 
GCCACATTAG TAGAGTACCA GGTTTTTGAC TCATTAAGGT TATCCACTAA GCAATTTGAA 
AAGAAGTATC GAATTGATTT AATGGAGGAT AGGAGTTTAT CATTGTATAT GGAGAACTCT 
ATTGAGAACG ATTCTTCAGT ACAAGACTTA TTAGATGAGG AGTATACACA GCTGGTTGCT 
GATCGTGAGT TACTATGCAA ATTTATTTTC CCCAAAGGTG ATGCTAGATG GCCTTTACCT 
GTCAATGTAC AAAGAATCAT CCAAAATGCT TTACAAATAT TCCATTTAGA AGCTAAAAAA 
CCCACCGATC TTTTACCGAG TGATATTATT AACGGGTTAA ATGAACTAAT TGCAAAATTA 
ACAATTTTCC GCGGAAGTGA CCGTATTACT CGTGATGTTC AAAACAACGC TACCTTGTTA 
TTCCAGATTT TATTAAGGTC CAAATTTGCT GTAAAACGGG TAATAATGGA ATACCGACTT 
AACAAAGTCG CATTTGAATG GATTATGGGT GAAGTGGAAG CTCGTTTCCA ACAAGCTGTC 
GTAAGTCCTG GAGAAATGGT GGGTACTCTG GCTGCACAAT CTATTGGAGA ACCAGCAACT 
CAAATGACAC TCAATACATT CCATTACGCT GGTGTTTCTT CTAAGAACGT TACCTTGGGT 
GTTCCTCGTT TGAAAGAAAT TTTGAATGTC GCTAAAAATA TTAAGACCCC TTCTTTAACT 
ATTTATCTTA TGCCCTGGAT AGCAGCTAAT ATGGATCTTG CTAAGAACGT TCAAACCCAA 
ATCGAACATA CAACTTTGAG CACTGTTACC TCTGCAACCG AAATTCATTA CGACCCAGAT 
CCTCAAGACA CAGTGATTGA AGAAGATAAG GATTTTGTTG AAGCTTTCTT TGCTATTCCT 
GATGAAGAAG TTGAAGAGAA CTTGTATAAG CAGTCTCCTT GGTTGCTTCG TCTTGAACTT 
GACCGTGCTA AGATGTTAGA TAAGAAGTTG AGTATGAGTG ATGTTGCTGG TAAAATTGCT 
GAAAGCTTTG AACGTGATCT TTTTACTATT TGGTCTGAGG ATAATGCAGA CAAGCTTATC 
ATTCGTTGTC GTATCATTCG CGATGATGAC CGTAAGGCAG AAGATGACGA TAATATGATT 
GAAGAGGATG TTTTTTTGAA AACTATTGAA GGTCATATGC TTGAGAGTAT TAGTCTTCGT 
GGTGTGCCGA ACATTACTCG TGTTTATATG ATGGAGCACA AGATTGTGCG GCAAATTGAA 
GATGGTACTT TTGAACGTGC TGATGAATGG GTTTTGGAAA CAGACGGCAT AAATCTTACT 
GAAGCAATGA CTGTAGAGGG TGTAGATGCC ACCAGAACTT ACTCCAATTC TTTCGTGGAA 
ATTTTGCAAA TTCTTGGTAT TGAAGCTACG AGATCTGCTT TACTTAAAGA ATTAAGAAAT 
GTTATCGAAT TCGATGGTTC TTACGTTAAT TATCGCCATC TGGCCCTTCT TTGCGATGTT 
ATGACATCTA GGGGCCATTT AATGGCTATT ACCCGTCATG GCATTAACAG AGCTGAAACC 
GGTGCTCTAA TGAGGTGCTC TTTTGAAGAA ACTGTAGAAA TCCTTATGGA TGCTGCTGCG 
AGTGGAGAAA AGGATGATTG CAAGGGAATA TCTGAAAACA TAATGCTAGG ACAATTAGCC 
CCAATGGGAA CTGGCGCATT TGATATTTAC CTTGATCAAG ATATGTTGAT GAATTACAGT 
CTTGGTACCG CCGTCCCTAC GCTCGCTGGG TCAGGAATGG GTACTTCCCA ATTACCAGAA 
GGAGCCGGTA CGCCATATGA ACGCTCACCA ATGGTTGATT CTGGATTTGT TGGATCTCCT 
GACGCCGCAG CATTTTCCCC TCTAGTACAA GGTGGATCCG AAGGTCGTGA AGGGTTTGGC 
GATTATGGAT TGTTGGGGGC TGCTAGTCCT TATAAAGGGG TACAATCCCC TGGTTATACT 
AGTCCATTTT CGTCTGCTAT GAGTCCTGGG TATGGACTTA CTTCACCAAG CTATAGTCCA 
TCATCTCCGG GATATTCCAC GTCACCTGCT TATATGCCAT CGAGTCCTTC CTATTCTCCA 
ACTAGTCCTT CTTATTCCCC TACTAGTCCT TCTTATTCCC CTACTAGTCC TTCCTATTCT 
CCAACAAGTC CTTCATACTC AGCGACAAGT CCATCCTACT CTCCAACTAG TCCCTCCTAT 
TCTCCTACTA GTCCTTCTTA TTCGCCTACA AGCCCATCAT ATTCTCCTAC TAGTCCCTCT 
TATTCACCGA CTAGTCCTTC TTATTCTCCC ACAAGCCCAT CATATTCTCC TACTAGTCCC 
TCTTATTCAC CGACTAGTCC TTCTTATTCT CCCACAAGTC CTTCTTATTC TCCTACGAGC 
CCATCGTATT CGCCTACTAG TCCTTCCTAT TCTCCTACGA GCCCGTCGTA TTCACCGACT 
AGTCCCTCTT ATTCACCGAC TAGTCCTTCT TACTCTCCAA CTAGTCCCTC TTATTCCCCT 
ACTAGTCCCT CTTATTCTCC TACTAGTCCT TCATATTCTC CTACGAGCCC TTCTTACTCT 
CCCACGAGTC CCTCGTATTC CCCTACTAGC CCATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595673.1
CDS	35..5293
Translation	MSGIQFSPSSVPLRRVEEVQFGILSPEEIRSMSVAKIEFPETMDESGQRPRVGGLLDPRLGTIDRQFKCQTCGETMADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCGKLKIDSSNPKFNDTQRYRDPKNRLNAVWNVCKTKMVCDTGLSAGSDNFDLSNPSANMGHGGCGAAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSPLEVHTIFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVSEYEQLLQFHVATYMDNEIAGQPQALQKSGRPLKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVTPYNIYQLQELVRNGPDEHPGAKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNRQPSLHKMSMMGHRIRVMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPVMGIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVILKPKVLWTGKQILSLIIPKGINLIRDDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHTIWKEKGPEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVKEARRQVAECIQDAQHNRLKPEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMVAAGSKGSFINISQMSACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKAMEDVMVRYDGTVRNAMGDIIQFAYGEDGLDATLVEYQVFDSLRLSTKQFEKKYRIDLMEDRSLSLYMENSIENDSSVQDLLDEEYTQLVADRELLCKFIFPKGDARWPLPVNVQRIIQNALQIFHLEAKKPTDLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQILLRSKFAVKRVIMEYRLNKVAFEWIMGEVEARFQQAVVSPGEMVGTLAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPWIAANMDLAKNVQTQIEHTTLSTVTSATEIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEENLYKQSPWLLRLELDRAKMLDKKLSMSDVAGKIAESFERDLFTIWSEDNADKLIIRCRIIRDDDRKAEDDDNMIEEDVFLKTIEGHMLESISLRGVPNITRVYMMEHKIVRQIEDGTFERADEWVLETDGINLTEAMTVEGVDATRTYSNSFVEILQILGIEATRSALLKELRNVIEFDGSYVNYRHLALLCDVMTSRGHLMAITRHGINRAETGALMRCSFEETVEILMDAAASGEKDDCKGISENIMLGQLAPMGTGAFDIYLDQDMLMNYSLGTAVPTLAGSGMGTSQLPEGAGTPYERSPMVDSGFVGSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS

Target ORF information:

RefSeq Version	NM_001021568.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RNA polymerase II large subunit Rpb1 (rpb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021568.2

ORF Insert Sequence:

ATGAGCGGGA TTCAGTTTTC ACCTTCTTCT GTCCCCTTAC GCCGGGTGGA GGAGGTTCAA 
TTTGGAATCT TGTCCCCGGA GGAAATTCGT TCAATGAGCG TTGCGAAGAT TGAGTTTCCT 
GAGACCATGG ATGAAAGTGG ACAACGCCCC CGTGTTGGTG GGCTCCTGGA CCCTCGATTG 
GGGACTATTG ATCGACAATT CAAGTGTCAA ACCTGTGGTG AAACTATGGC GGATTGTCCT 
GGTCATTTTG GTCATATTGA ACTTGCAAAG CCAGTTTTCC ACATCGGTTT CCTAAGCAAA 
ATAAAAAAAA TATTAGAATG CGTTTGTTGG AATTGCGGCA AACTTAAGAT TGATTCTTCA 
AATCCAAAAT TTAATGACAC ACAACGTTAT CGTGATCCCA AAAATCGATT AAATGCAGTT 
TGGAATGTCT GCAAGACAAA AATGGTTTGC GATACTGGCT TATCTGCAGG CTCAGATAAT 
TTCGACCTTA GTAATCCCTC CGCCAATATG GGACACGGTG GTTGTGGGGC TGCCCAACCT 
ACAATACGTA AAGATGGCCT GAGATTATGG GGTTCTTGGA AACGCGGTAA AGATGAATCA 
GATTTACCAG AAAAACGCCT TCTGTCGCCG TTGGAGGTGC ACACAATATT CACACATATT 
TCTTCAGAAG ATTTAGCTCA CTTAGGTCTT AATGAGCAGT ATGCTAGACC TGACTGGATG 
ATTATTACAG TCTTACCTGT TCCTCCTCCG AGTGTCCGTC CTAGTATTTC GGTGGATGGA 
ACCAGTCGTG GCGAAGATGA TTTAACGCAC AAACTTTCCG ATATCATAAA AGCAAATGCC 
AACGTTCGAC GGTGTGAACA AGAAGGAGCT CCTGCGCATA TTGTCTCTGA ATATGAACAG 
TTACTTCAGT TCCATGTTGC CACGTATATG GACAATGAAA TAGCGGGACA GCCTCAGGCT 
TTACAAAAAT CTGGCAGACC TCTTAAAAGT ATCCGTGCAC GGCTCAAGGG AAAAGAGGGT 
CGATTACGTG GCAACCTGAT GGGTAAACGG GTTGATTTTT CAGCTCGTAC TGTGATTACC 
GGTGATCCAA ATTTGTCGTT AGATGAACTC GGTGTTCCAC GTAGTATTGC TAAGACACTT 
ACTTATCCTG AAACTGTTAC ACCTTATAAT ATTTATCAAT TGCAAGAATT AGTACGAAAT 
GGTCCTGATG AGCATCCCGG TGCTAAATAT ATCATTCGTG ATACAGGAGA GCGTATTGAC 
TTGCGTTATC ATAAACGTGC TGGTGATATT CCTCTTAGGT ACGGTTGGCG AGTTGAGCGG 
CATATTCGTG ATGGCGATGT CGTTATTTTT AACAGACAGC CATCCCTTCA CAAAATGAGT 
ATGATGGGTC ATAGGATTCG CGTAATGCCG TACTCGACAT TCCGATTAAA CTTGTCAGTT 
ACGTCTCCTT ATAATGCTGA CTTTGATGGT GATGAAATGA ATATGCATGT CCCTCAGTCA 
GAAGAAACTC GTGCAGAAAT TCAGGAAATT ACTATGGTAC CTAAGCAGAT TGTTTCTCCT 
CAATCAAACA AGCCTGTCAT GGGTATTGTT CAAGATACTT TAGCAGGTGT TCGTAAGTTT 
TCGTTACGTG ATAACTTTTT GACTCGCAAC GCTGTAATGA ATATTATGTT ATGGGTACCA 
GACTGGGATG GAATACTCCC ACCACCCGTC ATTCTTAAAC CAAAGGTTTT ATGGACAGGA 
AAGCAAATTT TGAGCTTGAT TATACCCAAG GGAATTAACT TAATTAGAGA CGACGATAAG 
CAAAGTCTTT CAAATCCCAC CGACTCAGGA ATGTTGATTG AAAATGGTGA AATTATATAC 
GGCGTGGTTG ACAAGAAGAC TGTTGGTGCT TCTCAAGGTG GTTTAGTTCA TACTATCTGG 
AAAGAGAAAG GTCCTGAGAT TTGTAAAGGT TTCTTCAATG GTATTCAACG AGTTGTAAAC 
TATTGGCTTT TACATAACGG TTTTAGCATT GGTATAGGAG ATACTATTGC CGATGCGGAC 
ACAATGAAAG AGGTTACTAG AACTGTTAAG GAAGCCCGTC GTCAAGTCGC AGAATGTATT 
CAGGATGCTC AGCACAATCG CTTAAAGCCT GAGCCAGGAA TGACCCTTCG AGAATCTTTT 
GAAGCTAAGG TATCAAGGAT TCTCAATCAA GCGAGAGACA ACGCTGGTAG GTCAGCTGAG 
CATAGCTTGA AGGATTCTAA CAATGTCAAA CAAATGGTTG CTGCTGGTTC AAAAGGTTCA 
TTTATTAATA TCTCTCAAAT GTCTGCTTGC GTCGGTCAGC AAATCGTTGA AGGCAAACGT 
ATTCCTTTTG GATTTAAATA TCGTACATTA CCACATTTTC CTAAAGACGA CGACTCTCCA 
GAATCTCGTG GATTTATTGA AAATTCATAC TTAAGGGGTT TAACTCCTCA AGAGTTCTTT 
TTCCACGCAA TGGCTGGACG TGAGGGTTTG ATTGATACTG CGGTCAAAAC TGCTGAAACT 
GGATACATCC AAAGGCGTCT TGTTAAGGCT ATGGAGGATG TAATGGTTCG CTATGACGGT 
ACTGTGCGCA ATGCCATGGG TGACATTATA CAATTTGCCT ATGGTGAAGA TGGCCTGGAT 
GCCACATTAG TAGAGTACCA GGTTTTTGAC TCATTAAGGT TATCCACTAA GCAATTTGAA 
AAGAAGTATC GAATTGATTT AATGGAGGAT AGGAGTTTAT CATTGTATAT GGAGAACTCT 
ATTGAGAACG ATTCTTCAGT ACAAGACTTA TTAGATGAGG AGTATACACA GCTGGTTGCT 
GATCGTGAGT TACTATGCAA ATTTATTTTC CCCAAAGGTG ATGCTAGATG GCCTTTACCT 
GTCAATGTAC AAAGAATCAT CCAAAATGCT TTACAAATAT TCCATTTAGA AGCTAAAAAA 
CCCACCGATC TTTTACCGAG TGATATTATT AACGGGTTAA ATGAACTAAT TGCAAAATTA 
ACAATTTTCC GCGGAAGTGA CCGTATTACT CGTGATGTTC AAAACAACGC TACCTTGTTA 
TTCCAGATTT TATTAAGGTC CAAATTTGCT GTAAAACGGG TAATAATGGA ATACCGACTT 
AACAAAGTCG CATTTGAATG GATTATGGGT GAAGTGGAAG CTCGTTTCCA ACAAGCTGTC 
GTAAGTCCTG GAGAAATGGT GGGTACTCTG GCTGCACAAT CTATTGGAGA ACCAGCAACT 
CAAATGACAC TCAATACATT CCATTACGCT GGTGTTTCTT CTAAGAACGT TACCTTGGGT 
GTTCCTCGTT TGAAAGAAAT TTTGAATGTC GCTAAAAATA TTAAGACCCC TTCTTTAACT 
ATTTATCTTA TGCCCTGGAT AGCAGCTAAT ATGGATCTTG CTAAGAACGT TCAAACCCAA 
ATCGAACATA CAACTTTGAG CACTGTTACC TCTGCAACCG AAATTCATTA CGACCCAGAT 
CCTCAAGACA CAGTGATTGA AGAAGATAAG GATTTTGTTG AAGCTTTCTT TGCTATTCCT 
GATGAAGAAG TTGAAGAGAA CTTGTATAAG CAGTCTCCTT GGTTGCTTCG TCTTGAACTT 
GACCGTGCTA AGATGTTAGA TAAGAAGTTG AGTATGAGTG ATGTTGCTGG TAAAATTGCT 
GAAAGCTTTG AACGTGATCT TTTTACTATT TGGTCTGAGG ATAATGCAGA CAAGCTTATC 
ATTCGTTGTC GTATCATTCG CGATGATGAC CGTAAGGCAG AAGATGACGA TAATATGATT 
GAAGAGGATG TTTTTTTGAA AACTATTGAA GGTCATATGC TTGAGAGTAT TAGTCTTCGT 
GGTGTGCCGA ACATTACTCG TGTTTATATG ATGGAGCACA AGATTGTGCG GCAAATTGAA 
GATGGTACTT TTGAACGTGC TGATGAATGG GTTTTGGAAA CAGACGGCAT AAATCTTACT 
GAAGCAATGA CTGTAGAGGG TGTAGATGCC ACCAGAACTT ACTCCAATTC TTTCGTGGAA 
ATTTTGCAAA TTCTTGGTAT TGAAGCTACG AGATCTGCTT TACTTAAAGA ATTAAGAAAT 
GTTATCGAAT TCGATGGTTC TTACGTTAAT TATCGCCATC TGGCCCTTCT TTGCGATGTT 
ATGACATCTA GGGGCCATTT AATGGCTATT ACCCGTCATG GCATTAACAG AGCTGAAACC 
GGTGCTCTAA TGAGGTGCTC TTTTGAAGAA ACTGTAGAAA TCCTTATGGA TGCTGCTGCG 
AGTGGAGAAA AGGATGATTG CAAGGGAATA TCTGAAAACA TAATGCTAGG ACAATTAGCC 
CCAATGGGAA CTGGCGCATT TGATATTTAC CTTGATCAAG ATATGTTGAT GAATTACAGT 
CTTGGTACCG CCGTCCCTAC GCTCGCTGGG TCAGGAATGG GTACTTCCCA ATTACCAGAA 
GGAGCCGGTA CGCCATATGA ACGCTCACCA ATGGTTGATT CTGGATTTGT TGGATCTCCT 
GACGCCGCAG CATTTTCCCC TCTAGTACAA GGTGGATCCG AAGGTCGTGA AGGGTTTGGC 
GATTATGGAT TGTTGGGGGC TGCTAGTCCT TATAAAGGGG TACAATCCCC TGGTTATACT 
AGTCCATTTT CGTCTGCTAT GAGTCCTGGG TATGGACTTA CTTCACCAAG CTATAGTCCA 
TCATCTCCGG GATATTCCAC GTCACCTGCT TATATGCCAT CGAGTCCTTC CTATTCTCCA 
ACTAGTCCTT CTTATTCCCC TACTAGTCCT TCTTATTCCC CTACTAGTCC TTCCTATTCT 
CCAACAAGTC CTTCATACTC AGCGACAAGT CCATCCTACT CTCCAACTAG TCCCTCCTAT 
TCTCCTACTA GTCCTTCTTA TTCGCCTACA AGCCCATCAT ATTCTCCTAC TAGTCCCTCT 
TATTCACCGA CTAGTCCTTC TTATTCTCCC ACAAGCCCAT CATATTCTCC TACTAGTCCC 
TCTTATTCAC CGACTAGTCC TTCTTATTCT CCCACAAGTC CTTCTTATTC TCCTACGAGC 
CCATCGTATT CGCCTACTAG TCCTTCCTAT TCTCCTACGA GCCCGTCGTA TTCACCGACT 
AGTCCCTCTT ATTCACCGAC TAGTCCTTCT TACTCTCCAA CTAGTCCCTC TTATTCCCCT 
ACTAGTCCCT CTTATTCTCC TACTAGTCCT TCATATTCTC CTACGAGCCC TTCTTACTCT 
CCCACGAGTC CCTCGTATTC CCCTACTAGC CCATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rpb1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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