gRNAs
The following POLR2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa225988 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024979630.1 , NM_001206313.2 | |
| Accession Version | NM_001206313.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-27 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA-directed RNA polymerase II subunit RPB1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On Jun 28, 2018 this sequence version replaced NM_001206313.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.44616.1, SRR5571261.2719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001193242.1 |
| CDS | 1..5913 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001206313.2 |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa225987 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024979629.1 | |
| Accession Version | XM_024979629.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA-directed RNA polymerase II subunit RPB1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024835397.1 |
| CDS | 422..6346 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024979629.1 |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa225988 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024979630.1 , NM_001206313.2 | |
| Accession Version | XM_024979630.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA-directed RNA polymerase II subunit RPB1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024835398.1 |
| CDS | 422..6334 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024979630.1 |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa01603 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001206313.1 | |
| Accession Version | NM_001206313.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2011-10-22 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA-directed RNA polymerase II subunit RPB1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03100677.1. On Apr 20, 2011 this sequence version replaced XM_876181.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. | |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001193242.1 |
| CDS | 1..5913 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001206313.1 |
1 | ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
