pola-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	pola-1
Entrez Gene ID	176544
Full Name	DNA polymerase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001268180.1	NP_001255109.1	DNA polymerase
NM_001268180.2	NP_001255109.1	DNA polymerase
NM_001268179.1	NP_001255108.1	DNA polymerase
NM_001268179.2	NP_001255108.1	DNA polymerase

Pathways from BioSystems

Homologies

	POLA1	XP_001091195.1
Gallus gallus (chicken)	POLA1	XP_416792.3
Eremothecium gossypii	AGOS_AER381C	NP_985237.1
Homo sapiens (human)	POLA1	NP_058633.2
Rattus norvegicus (Norway rat)	Pola1	NP_445931.1
Schizosaccharomyces pombe (fission yeast)	pol1	NP_594186.1
Pan troglodytes (chimpanzee)	POLA1	XP_003317445.2
Mus musculus (house mouse)	Pola1	NP_032918.1
Danio rerio (zebrafish)	pola1	XP_005162809.1
Drosophila melanogaster (fruit fly)	DNApol-alpha180	NP_536736.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001929	XP_321134.5
Caenorhabditis elegans (roundworm)	Y47D3A.29	NP_001255109.1
Magnaporthe oryzae (rice blast fungus)	MGG_06397	XP_003717174.1
Neurospora crassa	NCU07870	XP_963131.2
Arabidopsis thaliana (thale cress)	ICU2	NP_201511.2
Xenopus tropicalis (tropical clawed frog)	pola1	XP_004911812.1
Bos taurus (cattle)	POLA1	NP_001192994.1
Saccharomyces cerevisiae (baker's yeast)	POL1	NP_014297.3
Kluyveromyces lactis	KLLA0C13255g	XP_452791.1
Canis lupus familiaris (dog)	POLA1	XP_005641271.1
Oryza sativa (rice)	Os01g0868300	NP_001044920.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pola-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340183	NM_001268180.2 Latest version!	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa340420	NM_001268179.2 Latest version!	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OCa340183	NM_001268180.1	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa340420	NM_001268179.1	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pola-1 ( NM_001268180.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255109.1 Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

Price & Availability↑

CloneID	OCa340183
Clone ID Related Accession (Same CDS sequence)	NM_001268180.1 , NM_001268180.2
Accession Version	NM_001268180.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAAA 
GCCGCCAAAG TCCGTGTCGA ATATAATGGG TGCAAAATCG ATTCAATGAT GTTGGCTGAT 
CGAATCAATG AGAAATATTT GGAGAAAAAT GCCTATAATC GAGTGGATCT TTCATATATT 
TTTGCGCCAA TGATGAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255109.1
CDS	1..4287
Translation	MSDSENHAESDVRRSSRRCGVSQKKSKELSAIEQLKLARSTGKAYRPELDVEDVYDTVDDDEYEKIVAKRQKDNFIVDDDGMGYVDNGVDFEDDYEDDYAPSSSKNSNSSKKGEKKPKKDKKKGGLHAFLSTTSTKKPIDESVVKAEFDDDEEVKNMLEQLGSSDEEAEEKPKATPANPFKRGRKFDSPSGSDSLAPRPIAKKAKREATGLAEHKFQRHVKVDEDEDYGIPDDYPVASPVTSPVKTEKMAEVEAPTAPAIQKIEETVKMETEHLEEVEDSLAKFRVIEDWTGGSSSQEDEDVKVEVKTGSEAFYTMGGEDNTTKAIRMYWIDAYEDVHKANGTVYLFGKVKTSGNAWETCSIVVKNICRKVYFMPRTKNLSTGEESSLAELHKEIGDVLRNKFNTHEFKCKMVEKELIRDESFGYGCGTKSPLMEVLYPAEKGKLPSDLKGDTFSNTFNTSVTPLERLLIEKKFMGPGWIELYNYSDPKAKTSNCKYEFEVDMEKMRNIKYLESSDAPPPTIKLLALNVVTTLNDRKDNEICMISMLFNPKCDLAHPSGDSKDLKPKCLVTKPPGGSLPYDIQKRLETEKLSKFVKSVANEKALLTLFLAMINEDEPDMIVGHDLSATIALLVSRLEKLKLPNWSRISRLKRSINIGKIGHSKSGQWELTAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLYVDSKDLISLINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDSDEQLSVPQSTDIEGVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEKMGYSVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVNKCHRLLELDLDGVFKRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKILDASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSGKFNLRHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPIEETDAVRIAENLGLDSTNYRRAAAAQASQRAAEEDCAWHQENYDLCEGIHVICPLDGCGHSNVIRETFDCVTDASSPRLLFSGCAQCQGSWDLQEHQAVIGNQVDRQMSEFVARHHLAAFKCDEPTCEFKTRVQTMKWCREGLECIRCSTGVLRREYTSKQLFDQQMFFRTILDVDAAVRKLSDAQKKAAKVRVEYNGCKIDSMMLADRINEKYLEKNAYNRVDLSYIFAPMMKIQ

Target ORF information:

RefSeq Version	NM_001268180.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268180.1

ORF Insert Sequence:

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAAA 
GCCGCCAAAG TCCGTGTCGA ATATAATGGG TGCAAAATCG ATTCAATGAT GTTGGCTGAT 
CGAATCAATG AGAAATATTT GGAGAAAAAT GCCTATAATC GAGTGGATCT TTCATATATT 
TTTGCGCCAA TGATGAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola-1 ( NM_001268180.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255109.1 Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

Price & Availability↑

CloneID	OCa340183
Clone ID Related Accession (Same CDS sequence)	NM_001268180.1 , NM_001268180.2
Accession Version	NM_001268180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268180.1. COMPLETENESS: incomplete on the 5' end.

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAAA 
GCCGCCAAAG TCCGTGTCGA ATATAATGGG TGCAAAATCG ATTCAATGAT GTTGGCTGAT 
CGAATCAATG AGAAATATTT GGAGAAAAAT GCCTATAATC GAGTGGATCT TTCATATATT 
TTTGCGCCAA TGATGAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255109.1
CDS	1..4287
Translation	MSDSENHAESDVRRSSRRCGVSQKKSKELSAIEQLKLARSTGKAYRPELDVEDVYDTVDDDEYEKIVAKRQKDNFIVDDDGMGYVDNGVDFEDDYEDDYAPSSSKNSNSSKKGEKKPKKDKKKGGLHAFLSTTSTKKPIDESVVKAEFDDDEEVKNMLEQLGSSDEEAEEKPKATPANPFKRGRKFDSPSGSDSLAPRPIAKKAKREATGLAEHKFQRHVKVDEDEDYGIPDDYPVASPVTSPVKTEKMAEVEAPTAPAIQKIEETVKMETEHLEEVEDSLAKFRVIEDWTGGSSSQEDEDVKVEVKTGSEAFYTMGGEDNTTKAIRMYWIDAYEDVHKANGTVYLFGKVKTSGNAWETCSIVVKNICRKVYFMPRTKNLSTGEESSLAELHKEIGDVLRNKFNTHEFKCKMVEKELIRDESFGYGCGTKSPLMEVLYPAEKGKLPSDLKGDTFSNTFNTSVTPLERLLIEKKFMGPGWIELYNYSDPKAKTSNCKYEFEVDMEKMRNIKYLESSDAPPPTIKLLALNVVTTLNDRKDNEICMISMLFNPKCDLAHPSGDSKDLKPKCLVTKPPGGSLPYDIQKRLETEKLSKFVKSVANEKALLTLFLAMINEDEPDMIVGHDLSATIALLVSRLEKLKLPNWSRISRLKRSINIGKIGHSKSGQWELTAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLYVDSKDLISLINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDSDEQLSVPQSTDIEGVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEKMGYSVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVNKCHRLLELDLDGVFKRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKILDASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSGKFNLRHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPIEETDAVRIAENLGLDSTNYRRAAAAQASQRAAEEDCAWHQENYDLCEGIHVICPLDGCGHSNVIRETFDCVTDASSPRLLFSGCAQCQGSWDLQEHQAVIGNQVDRQMSEFVARHHLAAFKCDEPTCEFKTRVQTMKWCREGLECIRCSTGVLRREYTSKQLFDQQMFFRTILDVDAAVRKLSDAQKKAAKVRVEYNGCKIDSMMLADRINEKYLEKNAYNRVDLSYIFAPMMKIQ

Target ORF information:

RefSeq Version	NM_001268180.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268180.2

ORF Insert Sequence:

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAAA 
GCCGCCAAAG TCCGTGTCGA ATATAATGGG TGCAAAATCG ATTCAATGAT GTTGGCTGAT 
CGAATCAATG AGAAATATTT GGAGAAAAAT GCCTATAATC GAGTGGATCT TTCATATATT 
TTTGCGCCAA TGATGAAAAT TCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola-1 ( NM_001268179.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255108.1 Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

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CloneID	OCa340420
Clone ID Related Accession (Same CDS sequence)	NM_001268179.1 , NM_001268179.2
Accession Version	NM_001268179.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). COMPLETENESS: incomplete on both ends.

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAGT 
TCGTGTTTGC GAAATTTTAT TGGAAAAAAA TGGGAAAAAT CCCTAGAAAA TGGGAAATTT 
CGGCGAAATT CAGCCAAGAA TCAAGCCGCC AAAGTCCGTG TCGAATATAA TGGGTGCAAA 
ATCGATTCAA TGATGTTGGC TGATCGAATC AATGAGAAAT ATTTGGAGAA AAATGCCTAT 
AATCGAGTGG ATCTTTCATA TATTTTTGCG CCAATGATGA AAATTCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255108.1
CDS	1..4371
Translation	MSDSENHAESDVRRSSRRCGVSQKKSKELSAIEQLKLARSTGKAYRPELDVEDVYDTVDDDEYEKIVAKRQKDNFIVDDDGMGYVDNGVDFEDDYEDDYAPSSSKNSNSSKKGEKKPKKDKKKGGLHAFLSTTSTKKPIDESVVKAEFDDDEEVKNMLEQLGSSDEEAEEKPKATPANPFKRGRKFDSPSGSDSLAPRPIAKKAKREATGLAEHKFQRHVKVDEDEDYGIPDDYPVASPVTSPVKTEKMAEVEAPTAPAIQKIEETVKMETEHLEEVEDSLAKFRVIEDWTGGSSSQEDEDVKVEVKTGSEAFYTMGGEDNTTKAIRMYWIDAYEDVHKANGTVYLFGKVKTSGNAWETCSIVVKNICRKVYFMPRTKNLSTGEESSLAELHKEIGDVLRNKFNTHEFKCKMVEKELIRDESFGYGCGTKSPLMEVLYPAEKGKLPSDLKGDTFSNTFNTSVTPLERLLIEKKFMGPGWIELYNYSDPKAKTSNCKYEFEVDMEKMRNIKYLESSDAPPPTIKLLALNVVTTLNDRKDNEICMISMLFNPKCDLAHPSGDSKDLKPKCLVTKPPGGSLPYDIQKRLETEKLSKFVKSVANEKALLTLFLAMINEDEPDMIVGHDLSATIALLVSRLEKLKLPNWSRISRLKRSINIGKIGHSKSGQWELTAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLYVDSKDLISLINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDSDEQLSVPQSTDIEGVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEKMGYSVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVNKCHRLLELDLDGVFKRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKILDASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSGKFNLRHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPIEETDAVRIAENLGLDSTNYRRAAAAQASQRAAEEDCAWHQENYDLCEGIHVICPLDGCGHSNVIRETFDCVTDASSPRLLFSGCAQCQGSWDLQEHQAVIGNQVDRQMSEFVARHHLAAFKCDEPTCEFKTRVQTMKWCREGLECIRCSTGVLRREYTSKQLFDQQMFFRTILDVDAAVRKLSDAQKSSCLRNFIGKKWEKSLENGKFRRNSAKNQAAKVRVEYNGCKIDSMMLADRINEKYLEKNAYNRVDLSYIFAPMMKIQ

Target ORF information:

RefSeq Version	NM_001268179.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268179.1

ORF Insert Sequence:

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAGT 
TCGTGTTTGC GAAATTTTAT TGGAAAAAAA TGGGAAAAAT CCCTAGAAAA TGGGAAATTT 
CGGCGAAATT CAGCCAAGAA TCAAGCCGCC AAAGTCCGTG TCGAATATAA TGGGTGCAAA 
ATCGATTCAA TGATGTTGGC TGATCGAATC AATGAGAAAT ATTTGGAGAA AAATGCCTAT 
AATCGAGTGG ATCTTTCATA TATTTTTGCG CCAATGATGA AAATTCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pola-1 ( NM_001268179.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001255108.1 Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

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CloneID	OCa340420
Clone ID Related Accession (Same CDS sequence)	NM_001268179.1 , NM_001268179.2
Accession Version	NM_001268179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_001268179.1. COMPLETENESS: incomplete on the 5' end.

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAGT 
TCGTGTTTGC GAAATTTTAT TGGAAAAAAA TGGGAAAAAT CCCTAGAAAA TGGGAAATTT 
CGGCGAAATT CAGCCAAGAA TCAAGCCGCC AAAGTCCGTG TCGAATATAA TGGGTGCAAA 
ATCGATTCAA TGATGTTGGC TGATCGAATC AATGAGAAAT ATTTGGAGAA AAATGCCTAT 
AATCGAGTGG ATCTTTCATA TATTTTTGCG CCAATGATGA AAATTCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001255108.1
CDS	1..4371
Translation	MSDSENHAESDVRRSSRRCGVSQKKSKELSAIEQLKLARSTGKAYRPELDVEDVYDTVDDDEYEKIVAKRQKDNFIVDDDGMGYVDNGVDFEDDYEDDYAPSSSKNSNSSKKGEKKPKKDKKKGGLHAFLSTTSTKKPIDESVVKAEFDDDEEVKNMLEQLGSSDEEAEEKPKATPANPFKRGRKFDSPSGSDSLAPRPIAKKAKREATGLAEHKFQRHVKVDEDEDYGIPDDYPVASPVTSPVKTEKMAEVEAPTAPAIQKIEETVKMETEHLEEVEDSLAKFRVIEDWTGGSSSQEDEDVKVEVKTGSEAFYTMGGEDNTTKAIRMYWIDAYEDVHKANGTVYLFGKVKTSGNAWETCSIVVKNICRKVYFMPRTKNLSTGEESSLAELHKEIGDVLRNKFNTHEFKCKMVEKELIRDESFGYGCGTKSPLMEVLYPAEKGKLPSDLKGDTFSNTFNTSVTPLERLLIEKKFMGPGWIELYNYSDPKAKTSNCKYEFEVDMEKMRNIKYLESSDAPPPTIKLLALNVVTTLNDRKDNEICMISMLFNPKCDLAHPSGDSKDLKPKCLVTKPPGGSLPYDIQKRLETEKLSKFVKSVANEKALLTLFLAMINEDEPDMIVGHDLSATIALLVSRLEKLKLPNWSRISRLKRSINIGKIGHSKSGQWELTAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLYVDSKDLISLINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDSDEQLSVPQSTDIEGVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEKMGYSVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVNKCHRLLELDLDGVFKRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKILDASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSGKFNLRHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPIEETDAVRIAENLGLDSTNYRRAAAAQASQRAAEEDCAWHQENYDLCEGIHVICPLDGCGHSNVIRETFDCVTDASSPRLLFSGCAQCQGSWDLQEHQAVIGNQVDRQMSEFVARHHLAAFKCDEPTCEFKTRVQTMKWCREGLECIRCSTGVLRREYTSKQLFDQQMFFRTILDVDAAVRKLSDAQKSSCLRNFIGKKWEKSLENGKFRRNSAKNQAAKVRVEYNGCKIDSMMLADRINEKYLEKNAYNRVDLSYIFAPMMKIQ

Target ORF information:

RefSeq Version	NM_001268179.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase (pola-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001268179.2

ORF Insert Sequence:

ATGTCCGACT CGGAAAACCA TGCAGAATCG GATGTTAGAC GCTCGTCTCG CCGCTGTGGA 
GTCAGTCAAA AGAAGTCGAA GGAGCTGTCT GCAATTGAGC AGCTGAAATT GGCGCGATCA 
ACAGGAAAAG CCTATCGCCC AGAGCTCGAC GTGGAGGACG TCTACGACAC GGTTGATGAT 
GATGAGTACG AGAAAATCGT TGCAAAACGA CAAAAGGACA ATTTTATTGT CGATGATGAT 
GGAATGGGCT ACGTAGACAA CGGTGTAGAC TTTGAAGATG ACTACGAAGA CGATTATGCG 
CCCAGTTCTT CGAAAAATTC AAATTCCTCG AAAAAAGGCG AGAAAAAGCC GAAAAAAGAC 
AAGAAAAAGG GAGGACTTCA TGCATTCCTC TCCACAACCT CCACCAAGAA GCCGATCGAC 
GAGTCTGTGG TGAAAGCCGA GTTTGACGAC GATGAGGAGG TGAAAAATAT GCTGGAGCAG 
CTGGGAAGCA GCGATGAGGA GGCCGAGGAG AAGCCGAAGG CGACCCCAGC GAATCCGTTC 
AAGCGGGGAC GAAAATTCGA CTCACCGAGC GGCTCGGATT CACTGGCTCC ACGGCCGATT 
GCTAAGAAGG CCAAGAGAGA GGCGACCGGG CTCGCCGAGC ACAAATTCCA GCGGCATGTG 
AAGGTTGATG AGGATGAGGA TTATGGGATT CCAGATGATT ATCCAGTGGC TTCTCCGGTG 
ACTTCTCCAG TGAAAACGGA GAAAATGGCA GAAGTCGAAG CTCCAACAGC TCCAGCGATC 
CAGAAAATCG AAGAAACAGT GAAAATGGAG ACCGAGCATC TGGAAGAAGT CGAGGATAGT 
CTGGCGAAGT TCCGTGTCAT TGAAGACTGG ACAGGCGGGA GCAGCAGTCA GGAGGACGAA 
GATGTGAAGG TCGAGGTGAA AACCGGATCC GAAGCCTTCT ACACGATGGG CGGCGAGGAC 
AACACCACCA AAGCGATCCG TATGTACTGG ATCGATGCCT ACGAGGATGT TCACAAGGCC 
AACGGCACCG TCTATCTCTT CGGAAAGGTC AAAACCAGCG GTAACGCCTG GGAGACCTGC 
TCAATCGTTG TGAAGAACAT CTGCAGGAAG GTCTACTTTA TGCCTCGCAC CAAGAATCTC 
AGCACCGGAG AGGAGTCGAG CCTTGCCGAG CTTCACAAGG AGATCGGAGA TGTTCTGCGG 
AACAAGTTCA ACACACACGA GTTCAAGTGT AAGATGGTGG AGAAGGAGCT GATCCGTGAC 
GAGAGCTTCG GCTACGGATG CGGCACGAAG AGCCCGTTGA TGGAAGTGCT CTATCCGGCG 
GAGAAGGGGA AGCTTCCCAG TGATCTTAAG GGTGACACAT TCTCCAACAC CTTCAACACA 
TCAGTCACTC CGTTGGAACG GTTGCTTATT GAAAAGAAAT TTATGGGTCC TGGATGGATT 
GAGCTGTATA ACTATTCGGA TCCCAAAGCG AAGACCTCGA ATTGCAAGTA TGAGTTTGAG 
GTTGATATGG AGAAGATGCG GAATATCAAG TATCTGGAAT CCTCTGACGC ACCACCGCCG 
ACGATCAAGC TTCTCGCCCT GAACGTTGTC ACAACGCTCA ACGATCGGAA GGACAATGAG 
ATTTGCATGA TTTCGATGCT TTTCAATCCG AAATGCGACT TGGCTCATCC GAGTGGTGAT 
AGTAAGGATT TGAAGCCGAA ATGCCTCGTC ACAAAGCCTC CCGGTGGATC TCTCCCCTAT 
GACATCCAAA AGCGCTTGGA AACTGAGAAA CTCTCGAAAT TCGTGAAAAG TGTTGCTAAT 
GAGAAGGCAC TGCTCACCCT GTTCCTGGCG ATGATCAATG AAGATGAACC TGATATGATT 
GTGGGACACG ACTTGTCGGC GACAATTGCC TTGTTGGTTT CAAGACTTGA AAAGCTCAAG 
CTGCCGAATT GGTCTAGAAT TTCTCGTTTA AAACGGTCAA TTAATATTGG AAAAATCGGA 
CATTCAAAAT CAGGCCAATG GGAGCTCACA GCGGGGAGAA TGATGTTGGA CTCGAAGCTT 
GCCGCGATGG AACTTGTTAA ATCTCGAAGC TTTGATCTCA CAGAGCTCTC TCAACAGATC 
CTTGGAGCCG AGCGCCGTGA GATTTACGCC AATGAGATTC CGCAGTTGTA CGTGGATTCC 
AAAGATCTGA TCAGTCTGAT CAATTGGTCT TGGAACGATC TTCTACTATC ACTTCGGATC 
GTTGTTCGGC TTAACGCTCT GCCACTCTAC ATTCAAATCT CGCAGATTGT CGGTGGAATC 
GCTTCGAGAA CGATGATGGG AGGACGTGCC GAGAGGAATG AGTATCTGTT GCTTCACGCG 
TTCGAAAAAG CGGATCTCAT CGCACCGGAT AAGTATAATA GCTCGGAGAG CAAGAAAAAG 
AAGGAGGAGA GCCAAGAAGG AGACGAGAAG AAGAGTGGAA AAGCACAATA TTCGGGTGGT 
CTGGTGTTGG AGCCGAAGAA GGGACTCTAT GAGACATTGA TCTTGCTGCT CGATTTTAAT 
TCATTGTATC CAAGCATTAT TCAAGAGTAT AATATTTGTT ATACGACGTT GGAGTACTCG 
AAGGACAGCG ACGAGCAGCT CTCAGTACCA CAGAGCACAG ATATCGAAGG AGTTCTACCA 
CGTGAAATTC GAAAGCTTGT AGAATGCCGT CGAGACGTGA AATCTCTTAT GAAAAGCGAA 
CGAAATGAGG CAAAAAAGAA GCAAATGGAT ATTCGTCAAA TGGCTTTAAA ACTCACTGCC 
AACTCAATGT ACGGATGTCT TGGATTTCAG TATTCAAGGT TTTACGCGAA GCCTCTAGCG 
GCACTGGTAA CTGCAAAAGG ACGAGAAATT CTGATGCATT CAAAGGATCT TGTAGAGAAA 
ATGGGTTATT CTGTAGTCTA TGGAGATACG GATTCAATTA TGATTAATAC GAATTCAGTG 
GATTTGGTGG CGGCAAAGAA GCTTGGAAAT GAGATTAAAA AGGCTGTTAA CAAGTGTCAT 
CGGCTCTTGG AGCTTGATCT TGATGGAGTT TTTAAGCGAA TGTTGCTCTT GAAAAAGAAG 
AAGTACGCCG CGTTGACGAT TAATCCGGAT ACGAAGGTTG AGGCGAAAGA ATTGAAAGGA 
TTGGATATTG TTAGAAGAGA TTGGTCACAG TTGGCTAAGG AGACTGGAAC AGCCGTCGTC 
GACAAGATCC TTGACGCTTC ACTGACCCGC GACGAGATGG TCTCATCGAT CGATGATCTA 
CTGCGTGAAA TTCGTGCAAA ACTGGATTCT GGCACGGTTC CACTGGAAAT GTTCCAAATT 
AGCAAGCAGC TAACTCGAAA TCCTGAACAA TATGCCGATG TGAAGGCTCA ATGCCATGCG 
GCAGTTGCTC AGCGGCTTAA TAAAAGTGGA AAATTCAATT TGAGGCATAA TGATATTGTG 
GAATATGTCA TTTGTGAAGA TGGAACTGAT AATCCGGCAA CACAACGAGC CTATCATCGG 
ACTGAAATGG CTGAGAATTC GGCACTTAAA ATTGATCTCC TCTACTATCT GGCTCAACAA 
ATTCATCCAG TCGTATCGCG GCTCGTCGAG CCCATCGAGG AAACCGACGC AGTCCGAATT 
GCCGAAAATC TCGGTCTGGA TTCGACCAAT TATCGACGAG CTGCGGCGGC TCAAGCTTCA 
CAGCGAGCAG CAGAAGAAGA TTGCGCGTGG CACCAAGAGA ATTATGATTT GTGCGAAGGA 
ATTCATGTGA TTTGTCCACT CGATGGGTGC GGTCATTCAA ATGTGATTCG AGAGACATTT 
GATTGTGTGA CTGATGCCTC GTCTCCCCGC CTTCTATTCT CCGGATGTGC TCAATGTCAA 
GGATCATGGG ATCTTCAGGA GCATCAAGCA GTGATTGGGA ATCAAGTTGA CCGACAAATG 
TCGGAATTCG TCGCCAGACA TCATCTCGCA GCGTTTAAAT GCGATGAGCC AACGTGTGAA 
TTCAAAACTC GCGTACAAAC GATGAAATGG TGTCGAGAAG GACTCGAATG TATTCGATGC 
TCAACTGGTG TGCTCCGTCG TGAATACACT TCAAAACAGC TTTTCGATCA GCAAATGTTC 
TTTCGAACAA TTCTCGATGT CGACGCGGCT GTACGAAAGT TGAGCGACGC CCAGAAAAGT 
TCGTGTTTGC GAAATTTTAT TGGAAAAAAA TGGGAAAAAT CCCTAGAAAA TGGGAAATTT 
CGGCGAAATT CAGCCAAGAA TCAAGCCGCC AAAGTCCGTG TCGAATATAA TGGGTGCAAA 
ATCGATTCAA TGATGTTGGC TGATCGAATC AATGAGAAAT ATTTGGAGAA AAATGCCTAT 
AATCGAGTGG ATCTTTCATA TATTTTTGCG CCAATGATGA AAATTCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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pola-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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