pfas-1 cDNA ORF clone, Caenorhabditis elegans

The following pfas-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfas-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa340009 NM_065541.6
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Caenorhabditis elegans Probable phosphoribosylformylglycinamidine synthase (pfas-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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OCa340009 NM_065541.5 Caenorhabditis elegans Probable phosphoribosylformylglycinamidine synthase (pfas-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa340009
    Clone ID Related Accession (Same CDS sequence) NM_065541.6 , NM_065541.5
    Accession Version NM_065541.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Probable phosphoribosylformylglycinamidine synthase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065541.5.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTAAGT TTCATGTTAA GCTTTACGCG AAAGCGGTTG AGTCAAGAAA ATTGGATCAG 
    ATTCAAAAAG ATTTTGAGAA GAAGTTCAAT CGAAAAATTG ATGTTTCTGT CGAGTATTGC
    TACCATGTTA TTACTCAAGA GCCGGAGCTC ATTTCCTCCA ACTGGGAAAA GCTTGTTACT
    TTATTATCTC ATTCTCCATT TGAAACCTCT GTTTGGAAAG AATCGCAACT ACATCCGGAA
    CACGGAAAAA ATATTGAAAT CGGCCCTCGA ACAGCTGTGA AAACAGCTGC TTGCACAAAC
    ATCCTTAGCA TTTTCGAAAG TTCTGGTATC AAGAATGTGG AGAGAATTGA ACGAGGAATT
    CGGTATTTGG TGGAAGATGA TGTGGATGTT AATGAGTTTT TTGAAATTGC TGCGGACAAA
    ATGACAGAAG CAATTTATGG AAATGATGTG AAGTTTGACG ATGAGAGTCA TCAGATAGAA
    AAAGTATTTC TGATTGATGT CTTGGAATCA AAACAGAACT TGATTAAGGC AAATGAAGAA
    CTGGGGTTGG CTTTGGATCA ATTGGATCTT GATTTTTATT ACGATTTTTT TGTGAACAAA
    GTCAAAAAAA ACCCCACAGA CGTTGAACTT TTTGATTTGG CTCAATCGGA CTCTGAGCAT
    TCTCGTCATT GGTTCTTCCG TGGAGAAATA TGGATTGATG ATCGGAAGAG AGATGGATCT
    TTAATGAAAA CAATCAGAGA GACACTTGAC AGTTCAAATG ATAATAGTTT GATTGCTTTT
    TGCGATAATT CCAGCGCAAT TCGAGGATTC GAGAGTGTCT GCCGTCTTCG TCCAAACGAT
    CCAACTACTG TATCCCCAAT GATTGCCATA TTTCCACCAT CCCATCTCAT CTACTCTGCT
    GAAACTCACA ATTTCCCAAC TGCAGTTTGT CCATTCCAGG GTGCAACAAC TGGAACTGGC
    GGTAGAATTA GAGATATTCA TGCAACTGGA AGAGGGGCAT ATGAGATTGC TGGAACCGTT
    GGGTATTCCT TTGGGAATTT GAATTTACCG GGGCTTCCGT TGCCCTGGGA AGATGAGACA
    TTCGAGTATC CCACATCGAT CAGTGAACCA GCGAAGATAG CCATAGAGGC TTCCAATGGA
    GCATCAGATT ATGGTAACAA GTTCGGTGAG CCCGTGATTT CTGGATTTGC TCGATCATTT
    GGGCAGAGAC TAGAGAACGG AGAAAGATGT GAATACTTGA AGCCAATTAT GTTTTCTGGA
    GGAATTGGTG CAATAGACAA AGATGAAGTA CGAAAAGAGC CATGTGCTCC TCATCAAAAA
    GTCGTCAAAA TCGGCGGCCC CGTATACAGA ATTGGAGTCG GTGGTGGAGC TGCAAGTTCA
    GTTTCTGTTC AAGGAAATCG TGAAAATCAG TTGGACTTTG CTGCCGTGCA ACGTGGAGAT
    GCTGAAATGG GTGGTAAACT TCATCGAGTT GTTAGAGCAT GTGCAGAGAG AATTGGTGGA
    AATCCATTGA TGGCGATACA TGATCAAGGA GCTGGTGGAA ACGGAAATGT GATCAAAGAA
    TTGGTGGAGG GATGTGGTGT CACAGTGAAA AGTGACACAT TCCAGCTCGG TGATGAGAGT
    ATATCGTTGA GAGAACTATG GACAGCTGAG TATCAGGAAA ACGACGCAGC TCTTGTAGAT
    GCATCACTTC TCGATGCTCT CCAAACCATT TCCAAACGCG AAAAATGTCA TGTGTCAGTT
    GTTGGAGAAG TAGAAAAAGA GCAAAGAGTC AAGCTCCTTG GGAAATCTGG TGAAATTGCA
    GTTGACCTAG ATACCCGGCA ACTTGGAGAA CGAGAAAAGA AGGTGTTCAA GTTGAAAAGT
    GCCCCGAGGG TTTTGAAGAA ACTAGAGTTA CCAGAAAATC TGACAGTAAG AAAAGCTCTG
    AAAAGAGTTT TGATGTTGCC TTCAGTTGCT AGCAAAAGAT ATTTGACATG CAAAGTAGAC
    CGATCTGTAA CTGGATTGGT TGCTCAACAG CAATGTGTTG GACCTCTACA CACCCCACTT
    GCTGATGTAG CAGTCGTGGC TCTGTCACAT TTTGATACGG TTGGTGGAGC CGTGTCTCTT
    GGCGAACAAC CCATAAAAAT GCTAATCGAC GCTGAAAAAG GAGCCCGAAT GTGTATTGCG
    GAGACGATAA TGAACCTCAT TTGGGCACCA ATTACCGATT TAAAAGACGT AAAAATGTCG
    GGTAACTGGA TGTGGGCTGC AAAATGTGAC GGAGAAGGGG CTCGTTTAGT TGATGCTGTT
    GGAGCTCTTT GTCGTGGACT TCGTGAGATT GGATGTGCGA TTGACGGAGG AAAAGATTCG
    TTATCAATGG CGGTGACCGC CCATGGAGAA GTGGTCAAGA GTCCCGGTAC TCTTGTACTT
    TCCGCATATG CTCCTTGCAC AAATGTTACA AAAGTTGTGA ATCCATCGCT GAAAGCTGTT
    CCCGGTTCAA AAATCTTGTG GATCAAGATT GGAAGTTCCG AAGAGAAAAT GAGGCTTGGA
    GGAAGTGCAC TGGCACAAGT TTATTCACAG ATTGGAGACG ACTGTCCGGA TATTGAGAAC
    TTCTCGGAAA TTTCTAAAGT GTTCTCAATT GTTCAACAGT TACTGAATAG AGAAGAGCTG
    GCAGGGCCTT TGAGAAAACC AATTATTTTG GCAGGACATG ACATATCGGA TGGAGGACTC
    TTGACTGCTA TTCTGGAAAT GGCATTTGCT GGAAATGTTT CTATTGATAT TGATATCAAG
    CCCCCCAACC AGAATATCAA GCCTATCGAT ATTTTATTCG CTGAAGAATG TGGCATACTT
    TTGGAAGTTT CTAATCCTGA AAATGTTCTG CACATATTCT CTGAAGCTGG AATTAAATGC
    CAAGAAATTG GAAAAGCATC TGCCGTGTTT GGTCCAGATG CCCACGTAAA AATCCATGTG
    AATGGTCATT TGGAAATCAA TGAGAAGCTG GTTGATTTGA GAGAAGAATG GGAATTGGTT
    GGAGATCGAT TGGGAGAGTT TCAGACAAAT CCCAAGAGTT TGAAGGAAGC AAGAGAGGTT
    CGAAGAACAT GTCAGAAGAT CAATTATAAA TGTGATTTTG ATTGGTATTA TAATCCAGCG
    TTTATTCATA ATGAGCAGTA CTTTTCTACA GCCCCACGAG TCGCTATAAT CCGTGAAGAA
    GGTTCGAATG GTGATCGAGA AATGGCTTCT GCATTCACTC TTGCTGGATT CCAAACATTC
    GATGTCACAA TGACTGATAT TTTGGCTGGG CACACTTTGG AAGCTTACAG AGGTGTCGCG
    TTTGTGGGTG GATTTTCATA TGCTGATGTT CTGGGGTCTG CGAAAGGTTG GGCTGCCGGT
    GTTCAATTCA ACGAGTCTGT TTCAAAGCAA TTCGAAGCTT TCCGTTCGAG ACCTGACACA
    TTCTCATATG GAGTTTGTAA TGGATGTCAG TTGATGGCTC AATTGGGATG GATTGGGGAT
    GAAGAACAGA AAGGGCCTAC TGTTTTTTTG GATGAAAACG AATGTGGGAG ATTTGATAGC
    AGTTTTGGGC CAGTTAAAAT CGAAAAAAAC GTCAGCATTA TGCTTTCCGG AATGGAAAAC
    TCCGTTCTCG GCCTCTGGTC TTCTCATGGA GAGGGTCGCT TCACCTACCG TAACCTTCAA
    AACTTCCAAA ATCTGAAGAC AAATGGTCAA GTCTGTATTC GTTTTTGTGA TGATCGAGGA
    ATGACTGGCG CTGATCATGG ATCCGTGAAG CTACCGTACC CATGGAATCC CAATGGATCC
    ATTGATGACG TGGCAGCTAT TTGTAGTCGG GACGGACGTC ATCTTGCTAT GATGCCACAT
    GCTGACAGAT CATTCTTGAC GTGGCAATGG GCGGAGTCAA GTGAGGTTCC ATGGAATGCA
    AGATTCGATC AAAAGACAGT GGCACTTTCC CCATGGATCA AAATGTTTAG AAATGCATAT
    AATTGGTGTC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_497942.2
    CDS29..4003
    Translation

    Target ORF information:

    RefSeq Version NM_065541.6
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Probable phosphoribosylformylglycinamidine synthase (pfas-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_065541.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTAAGT TTCATGTTAA GCTTTACGCG AAAGCGGTTG AGTCAAGAAA ATTGGATCAG 
    ATTCAAAAAG ATTTTGAGAA GAAGTTCAAT CGAAAAATTG ATGTTTCTGT CGAGTATTGC
    TACCATGTTA TTACTCAAGA GCCGGAGCTC ATTTCCTCCA ACTGGGAAAA GCTTGTTACT
    TTATTATCTC ATTCTCCATT TGAAACCTCT GTTTGGAAAG AATCGCAACT ACATCCGGAA
    CACGGAAAAA ATATTGAAAT CGGCCCTCGA ACAGCTGTGA AAACAGCTGC TTGCACAAAC
    ATCCTTAGCA TTTTCGAAAG TTCTGGTATC AAGAATGTGG AGAGAATTGA ACGAGGAATT
    CGGTATTTGG TGGAAGATGA TGTGGATGTT AATGAGTTTT TTGAAATTGC TGCGGACAAA
    ATGACAGAAG CAATTTATGG AAATGATGTG AAGTTTGACG ATGAGAGTCA TCAGATAGAA
    AAAGTATTTC TGATTGATGT CTTGGAATCA AAACAGAACT TGATTAAGGC AAATGAAGAA
    CTGGGGTTGG CTTTGGATCA ATTGGATCTT GATTTTTATT ACGATTTTTT TGTGAACAAA
    GTCAAAAAAA ACCCCACAGA CGTTGAACTT TTTGATTTGG CTCAATCGGA CTCTGAGCAT
    TCTCGTCATT GGTTCTTCCG TGGAGAAATA TGGATTGATG ATCGGAAGAG AGATGGATCT
    TTAATGAAAA CAATCAGAGA GACACTTGAC AGTTCAAATG ATAATAGTTT GATTGCTTTT
    TGCGATAATT CCAGCGCAAT TCGAGGATTC GAGAGTGTCT GCCGTCTTCG TCCAAACGAT
    CCAACTACTG TATCCCCAAT GATTGCCATA TTTCCACCAT CCCATCTCAT CTACTCTGCT
    GAAACTCACA ATTTCCCAAC TGCAGTTTGT CCATTCCAGG GTGCAACAAC TGGAACTGGC
    GGTAGAATTA GAGATATTCA TGCAACTGGA AGAGGGGCAT ATGAGATTGC TGGAACCGTT
    GGGTATTCCT TTGGGAATTT GAATTTACCG GGGCTTCCGT TGCCCTGGGA AGATGAGACA
    TTCGAGTATC CCACATCGAT CAGTGAACCA GCGAAGATAG CCATAGAGGC TTCCAATGGA
    GCATCAGATT ATGGTAACAA GTTCGGTGAG CCCGTGATTT CTGGATTTGC TCGATCATTT
    GGGCAGAGAC TAGAGAACGG AGAAAGATGT GAATACTTGA AGCCAATTAT GTTTTCTGGA
    GGAATTGGTG CAATAGACAA AGATGAAGTA CGAAAAGAGC CATGTGCTCC TCATCAAAAA
    GTCGTCAAAA TCGGCGGCCC CGTATACAGA ATTGGAGTCG GTGGTGGAGC TGCAAGTTCA
    GTTTCTGTTC AAGGAAATCG TGAAAATCAG TTGGACTTTG CTGCCGTGCA ACGTGGAGAT
    GCTGAAATGG GTGGTAAACT TCATCGAGTT GTTAGAGCAT GTGCAGAGAG AATTGGTGGA
    AATCCATTGA TGGCGATACA TGATCAAGGA GCTGGTGGAA ACGGAAATGT GATCAAAGAA
    TTGGTGGAGG GATGTGGTGT CACAGTGAAA AGTGACACAT TCCAGCTCGG TGATGAGAGT
    ATATCGTTGA GAGAACTATG GACAGCTGAG TATCAGGAAA ACGACGCAGC TCTTGTAGAT
    GCATCACTTC TCGATGCTCT CCAAACCATT TCCAAACGCG AAAAATGTCA TGTGTCAGTT
    GTTGGAGAAG TAGAAAAAGA GCAAAGAGTC AAGCTCCTTG GGAAATCTGG TGAAATTGCA
    GTTGACCTAG ATACCCGGCA ACTTGGAGAA CGAGAAAAGA AGGTGTTCAA GTTGAAAAGT
    GCCCCGAGGG TTTTGAAGAA ACTAGAGTTA CCAGAAAATC TGACAGTAAG AAAAGCTCTG
    AAAAGAGTTT TGATGTTGCC TTCAGTTGCT AGCAAAAGAT ATTTGACATG CAAAGTAGAC
    CGATCTGTAA CTGGATTGGT TGCTCAACAG CAATGTGTTG GACCTCTACA CACCCCACTT
    GCTGATGTAG CAGTCGTGGC TCTGTCACAT TTTGATACGG TTGGTGGAGC CGTGTCTCTT
    GGCGAACAAC CCATAAAAAT GCTAATCGAC GCTGAAAAAG GAGCCCGAAT GTGTATTGCG
    GAGACGATAA TGAACCTCAT TTGGGCACCA ATTACCGATT TAAAAGACGT AAAAATGTCG
    GGTAACTGGA TGTGGGCTGC AAAATGTGAC GGAGAAGGGG CTCGTTTAGT TGATGCTGTT
    GGAGCTCTTT GTCGTGGACT TCGTGAGATT GGATGTGCGA TTGACGGAGG AAAAGATTCG
    TTATCAATGG CGGTGACCGC CCATGGAGAA GTGGTCAAGA GTCCCGGTAC TCTTGTACTT
    TCCGCATATG CTCCTTGCAC AAATGTTACA AAAGTTGTGA ATCCATCGCT GAAAGCTGTT
    CCCGGTTCAA AAATCTTGTG GATCAAGATT GGAAGTTCCG AAGAGAAAAT GAGGCTTGGA
    GGAAGTGCAC TGGCACAAGT TTATTCACAG ATTGGAGACG ACTGTCCGGA TATTGAGAAC
    TTCTCGGAAA TTTCTAAAGT GTTCTCAATT GTTCAACAGT TACTGAATAG AGAAGAGCTG
    GCAGGGCCTT TGAGAAAACC AATTATTTTG GCAGGACATG ACATATCGGA TGGAGGACTC
    TTGACTGCTA TTCTGGAAAT GGCATTTGCT GGAAATGTTT CTATTGATAT TGATATCAAG
    CCCCCCAACC AGAATATCAA GCCTATCGAT ATTTTATTCG CTGAAGAATG TGGCATACTT
    TTGGAAGTTT CTAATCCTGA AAATGTTCTG CACATATTCT CTGAAGCTGG AATTAAATGC
    CAAGAAATTG GAAAAGCATC TGCCGTGTTT GGTCCAGATG CCCACGTAAA AATCCATGTG
    AATGGTCATT TGGAAATCAA TGAGAAGCTG GTTGATTTGA GAGAAGAATG GGAATTGGTT
    GGAGATCGAT TGGGAGAGTT TCAGACAAAT CCCAAGAGTT TGAAGGAAGC AAGAGAGGTT
    CGAAGAACAT GTCAGAAGAT CAATTATAAA TGTGATTTTG ATTGGTATTA TAATCCAGCG
    TTTATTCATA ATGAGCAGTA CTTTTCTACA GCCCCACGAG TCGCTATAAT CCGTGAAGAA
    GGTTCGAATG GTGATCGAGA AATGGCTTCT GCATTCACTC TTGCTGGATT CCAAACATTC
    GATGTCACAA TGACTGATAT TTTGGCTGGG CACACTTTGG AAGCTTACAG AGGTGTCGCG
    TTTGTGGGTG GATTTTCATA TGCTGATGTT CTGGGGTCTG CGAAAGGTTG GGCTGCCGGT
    GTTCAATTCA ACGAGTCTGT TTCAAAGCAA TTCGAAGCTT TCCGTTCGAG ACCTGACACA
    TTCTCATATG GAGTTTGTAA TGGATGTCAG TTGATGGCTC AATTGGGATG GATTGGGGAT
    GAAGAACAGA AAGGGCCTAC TGTTTTTTTG GATGAAAACG AATGTGGGAG ATTTGATAGC
    AGTTTTGGGC CAGTTAAAAT CGAAAAAAAC GTCAGCATTA TGCTTTCCGG AATGGAAAAC
    TCCGTTCTCG GCCTCTGGTC TTCTCATGGA GAGGGTCGCT TCACCTACCG TAACCTTCAA
    AACTTCCAAA ATCTGAAGAC AAATGGTCAA GTCTGTATTC GTTTTTGTGA TGATCGAGGA
    ATGACTGGCG CTGATCATGG ATCCGTGAAG CTACCGTACC CATGGAATCC CAATGGATCC
    ATTGATGACG TGGCAGCTAT TTGTAGTCGG GACGGACGTC ATCTTGCTAT GATGCCACAT
    GCTGACAGAT CATTCTTGAC GTGGCAATGG GCGGAGTCAA GTGAGGTTCC ATGGAATGCA
    AGATTCGATC AAAAGACAGT GGCACTTTCC CCATGGATCA AAATGTTTAG AAATGCATAT
    AATTGGTGTC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa340009
    Clone ID Related Accession (Same CDS sequence) NM_065541.6 , NM_065541.5
    Accession Version NM_065541.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Probable phosphoribosylformylglycinamidine synthase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065541.4. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTAAGT TTCATGTTAA GCTTTACGCG AAAGCGGTTG AGTCAAGAAA ATTGGATCAG 
    ATTCAAAAAG ATTTTGAGAA GAAGTTCAAT CGAAAAATTG ATGTTTCTGT CGAGTATTGC
    TACCATGTTA TTACTCAAGA GCCGGAGCTC ATTTCCTCCA ACTGGGAAAA GCTTGTTACT
    TTATTATCTC ATTCTCCATT TGAAACCTCT GTTTGGAAAG AATCGCAACT ACATCCGGAA
    CACGGAAAAA ATATTGAAAT CGGCCCTCGA ACAGCTGTGA AAACAGCTGC TTGCACAAAC
    ATCCTTAGCA TTTTCGAAAG TTCTGGTATC AAGAATGTGG AGAGAATTGA ACGAGGAATT
    CGGTATTTGG TGGAAGATGA TGTGGATGTT AATGAGTTTT TTGAAATTGC TGCGGACAAA
    ATGACAGAAG CAATTTATGG AAATGATGTG AAGTTTGACG ATGAGAGTCA TCAGATAGAA
    AAAGTATTTC TGATTGATGT CTTGGAATCA AAACAGAACT TGATTAAGGC AAATGAAGAA
    CTGGGGTTGG CTTTGGATCA ATTGGATCTT GATTTTTATT ACGATTTTTT TGTGAACAAA
    GTCAAAAAAA ACCCCACAGA CGTTGAACTT TTTGATTTGG CTCAATCGGA CTCTGAGCAT
    TCTCGTCATT GGTTCTTCCG TGGAGAAATA TGGATTGATG ATCGGAAGAG AGATGGATCT
    TTAATGAAAA CAATCAGAGA GACACTTGAC AGTTCAAATG ATAATAGTTT GATTGCTTTT
    TGCGATAATT CCAGCGCAAT TCGAGGATTC GAGAGTGTCT GCCGTCTTCG TCCAAACGAT
    CCAACTACTG TATCCCCAAT GATTGCCATA TTTCCACCAT CCCATCTCAT CTACTCTGCT
    GAAACTCACA ATTTCCCAAC TGCAGTTTGT CCATTCCAGG GTGCAACAAC TGGAACTGGC
    GGTAGAATTA GAGATATTCA TGCAACTGGA AGAGGGGCAT ATGAGATTGC TGGAACCGTT
    GGGTATTCCT TTGGGAATTT GAATTTACCG GGGCTTCCGT TGCCCTGGGA AGATGAGACA
    TTCGAGTATC CCACATCGAT CAGTGAACCA GCGAAGATAG CCATAGAGGC TTCCAATGGA
    GCATCAGATT ATGGTAACAA GTTCGGTGAG CCCGTGATTT CTGGATTTGC TCGATCATTT
    GGGCAGAGAC TAGAGAACGG AGAAAGATGT GAATACTTGA AGCCAATTAT GTTTTCTGGA
    GGAATTGGTG CAATAGACAA AGATGAAGTA CGAAAAGAGC CATGTGCTCC TCATCAAAAA
    GTCGTCAAAA TCGGCGGCCC CGTATACAGA ATTGGAGTCG GTGGTGGAGC TGCAAGTTCA
    GTTTCTGTTC AAGGAAATCG TGAAAATCAG TTGGACTTTG CTGCCGTGCA ACGTGGAGAT
    GCTGAAATGG GTGGTAAACT TCATCGAGTT GTTAGAGCAT GTGCAGAGAG AATTGGTGGA
    AATCCATTGA TGGCGATACA TGATCAAGGA GCTGGTGGAA ACGGAAATGT GATCAAAGAA
    TTGGTGGAGG GATGTGGTGT CACAGTGAAA AGTGACACAT TCCAGCTCGG TGATGAGAGT
    ATATCGTTGA GAGAACTATG GACAGCTGAG TATCAGGAAA ACGACGCAGC TCTTGTAGAT
    GCATCACTTC TCGATGCTCT CCAAACCATT TCCAAACGCG AAAAATGTCA TGTGTCAGTT
    GTTGGAGAAG TAGAAAAAGA GCAAAGAGTC AAGCTCCTTG GGAAATCTGG TGAAATTGCA
    GTTGACCTAG ATACCCGGCA ACTTGGAGAA CGAGAAAAGA AGGTGTTCAA GTTGAAAAGT
    GCCCCGAGGG TTTTGAAGAA ACTAGAGTTA CCAGAAAATC TGACAGTAAG AAAAGCTCTG
    AAAAGAGTTT TGATGTTGCC TTCAGTTGCT AGCAAAAGAT ATTTGACATG CAAAGTAGAC
    CGATCTGTAA CTGGATTGGT TGCTCAACAG CAATGTGTTG GACCTCTACA CACCCCACTT
    GCTGATGTAG CAGTCGTGGC TCTGTCACAT TTTGATACGG TTGGTGGAGC CGTGTCTCTT
    GGCGAACAAC CCATAAAAAT GCTAATCGAC GCTGAAAAAG GAGCCCGAAT GTGTATTGCG
    GAGACGATAA TGAACCTCAT TTGGGCACCA ATTACCGATT TAAAAGACGT AAAAATGTCG
    GGTAACTGGA TGTGGGCTGC AAAATGTGAC GGAGAAGGGG CTCGTTTAGT TGATGCTGTT
    GGAGCTCTTT GTCGTGGACT TCGTGAGATT GGATGTGCGA TTGACGGAGG AAAAGATTCG
    TTATCAATGG CGGTGACCGC CCATGGAGAA GTGGTCAAGA GTCCCGGTAC TCTTGTACTT
    TCCGCATATG CTCCTTGCAC AAATGTTACA AAAGTTGTGA ATCCATCGCT GAAAGCTGTT
    CCCGGTTCAA AAATCTTGTG GATCAAGATT GGAAGTTCCG AAGAGAAAAT GAGGCTTGGA
    GGAAGTGCAC TGGCACAAGT TTATTCACAG ATTGGAGACG ACTGTCCGGA TATTGAGAAC
    TTCTCGGAAA TTTCTAAAGT GTTCTCAATT GTTCAACAGT TACTGAATAG AGAAGAGCTG
    GCAGGGCCTT TGAGAAAACC AATTATTTTG GCAGGACATG ACATATCGGA TGGAGGACTC
    TTGACTGCTA TTCTGGAAAT GGCATTTGCT GGAAATGTTT CTATTGATAT TGATATCAAG
    CCCCCCAACC AGAATATCAA GCCTATCGAT ATTTTATTCG CTGAAGAATG TGGCATACTT
    TTGGAAGTTT CTAATCCTGA AAATGTTCTG CACATATTCT CTGAAGCTGG AATTAAATGC
    CAAGAAATTG GAAAAGCATC TGCCGTGTTT GGTCCAGATG CCCACGTAAA AATCCATGTG
    AATGGTCATT TGGAAATCAA TGAGAAGCTG GTTGATTTGA GAGAAGAATG GGAATTGGTT
    GGAGATCGAT TGGGAGAGTT TCAGACAAAT CCCAAGAGTT TGAAGGAAGC AAGAGAGGTT
    CGAAGAACAT GTCAGAAGAT CAATTATAAA TGTGATTTTG ATTGGTATTA TAATCCAGCG
    TTTATTCATA ATGAGCAGTA CTTTTCTACA GCCCCACGAG TCGCTATAAT CCGTGAAGAA
    GGTTCGAATG GTGATCGAGA AATGGCTTCT GCATTCACTC TTGCTGGATT CCAAACATTC
    GATGTCACAA TGACTGATAT TTTGGCTGGG CACACTTTGG AAGCTTACAG AGGTGTCGCG
    TTTGTGGGTG GATTTTCATA TGCTGATGTT CTGGGGTCTG CGAAAGGTTG GGCTGCCGGT
    GTTCAATTCA ACGAGTCTGT TTCAAAGCAA TTCGAAGCTT TCCGTTCGAG ACCTGACACA
    TTCTCATATG GAGTTTGTAA TGGATGTCAG TTGATGGCTC AATTGGGATG GATTGGGGAT
    GAAGAACAGA AAGGGCCTAC TGTTTTTTTG GATGAAAACG AATGTGGGAG ATTTGATAGC
    AGTTTTGGGC CAGTTAAAAT CGAAAAAAAC GTCAGCATTA TGCTTTCCGG AATGGAAAAC
    TCCGTTCTCG GCCTCTGGTC TTCTCATGGA GAGGGTCGCT TCACCTACCG TAACCTTCAA
    AACTTCCAAA ATCTGAAGAC AAATGGTCAA GTCTGTATTC GTTTTTGTGA TGATCGAGGA
    ATGACTGGCG CTGATCATGG ATCCGTGAAG CTACCGTACC CATGGAATCC CAATGGATCC
    ATTGATGACG TGGCAGCTAT TTGTAGTCGG GACGGACGTC ATCTTGCTAT GATGCCACAT
    GCTGACAGAT CATTCTTGAC GTGGCAATGG GCGGAGTCAA GTGAGGTTCC ATGGAATGCA
    AGATTCGATC AAAAGACAGT GGCACTTTCC CCATGGATCA AAATGTTTAG AAATGCATAT
    AATTGGTGTC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_497942.2
    CDS1..3975
    Translation

    Target ORF information:

    RefSeq Version NM_065541.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Probable phosphoribosylformylglycinamidine synthase (pfas-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_065541.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACTAAGT TTCATGTTAA GCTTTACGCG AAAGCGGTTG AGTCAAGAAA ATTGGATCAG 
    ATTCAAAAAG ATTTTGAGAA GAAGTTCAAT CGAAAAATTG ATGTTTCTGT CGAGTATTGC
    TACCATGTTA TTACTCAAGA GCCGGAGCTC ATTTCCTCCA ACTGGGAAAA GCTTGTTACT
    TTATTATCTC ATTCTCCATT TGAAACCTCT GTTTGGAAAG AATCGCAACT ACATCCGGAA
    CACGGAAAAA ATATTGAAAT CGGCCCTCGA ACAGCTGTGA AAACAGCTGC TTGCACAAAC
    ATCCTTAGCA TTTTCGAAAG TTCTGGTATC AAGAATGTGG AGAGAATTGA ACGAGGAATT
    CGGTATTTGG TGGAAGATGA TGTGGATGTT AATGAGTTTT TTGAAATTGC TGCGGACAAA
    ATGACAGAAG CAATTTATGG AAATGATGTG AAGTTTGACG ATGAGAGTCA TCAGATAGAA
    AAAGTATTTC TGATTGATGT CTTGGAATCA AAACAGAACT TGATTAAGGC AAATGAAGAA
    CTGGGGTTGG CTTTGGATCA ATTGGATCTT GATTTTTATT ACGATTTTTT TGTGAACAAA
    GTCAAAAAAA ACCCCACAGA CGTTGAACTT TTTGATTTGG CTCAATCGGA CTCTGAGCAT
    TCTCGTCATT GGTTCTTCCG TGGAGAAATA TGGATTGATG ATCGGAAGAG AGATGGATCT
    TTAATGAAAA CAATCAGAGA GACACTTGAC AGTTCAAATG ATAATAGTTT GATTGCTTTT
    TGCGATAATT CCAGCGCAAT TCGAGGATTC GAGAGTGTCT GCCGTCTTCG TCCAAACGAT
    CCAACTACTG TATCCCCAAT GATTGCCATA TTTCCACCAT CCCATCTCAT CTACTCTGCT
    GAAACTCACA ATTTCCCAAC TGCAGTTTGT CCATTCCAGG GTGCAACAAC TGGAACTGGC
    GGTAGAATTA GAGATATTCA TGCAACTGGA AGAGGGGCAT ATGAGATTGC TGGAACCGTT
    GGGTATTCCT TTGGGAATTT GAATTTACCG GGGCTTCCGT TGCCCTGGGA AGATGAGACA
    TTCGAGTATC CCACATCGAT CAGTGAACCA GCGAAGATAG CCATAGAGGC TTCCAATGGA
    GCATCAGATT ATGGTAACAA GTTCGGTGAG CCCGTGATTT CTGGATTTGC TCGATCATTT
    GGGCAGAGAC TAGAGAACGG AGAAAGATGT GAATACTTGA AGCCAATTAT GTTTTCTGGA
    GGAATTGGTG CAATAGACAA AGATGAAGTA CGAAAAGAGC CATGTGCTCC TCATCAAAAA
    GTCGTCAAAA TCGGCGGCCC CGTATACAGA ATTGGAGTCG GTGGTGGAGC TGCAAGTTCA
    GTTTCTGTTC AAGGAAATCG TGAAAATCAG TTGGACTTTG CTGCCGTGCA ACGTGGAGAT
    GCTGAAATGG GTGGTAAACT TCATCGAGTT GTTAGAGCAT GTGCAGAGAG AATTGGTGGA
    AATCCATTGA TGGCGATACA TGATCAAGGA GCTGGTGGAA ACGGAAATGT GATCAAAGAA
    TTGGTGGAGG GATGTGGTGT CACAGTGAAA AGTGACACAT TCCAGCTCGG TGATGAGAGT
    ATATCGTTGA GAGAACTATG GACAGCTGAG TATCAGGAAA ACGACGCAGC TCTTGTAGAT
    GCATCACTTC TCGATGCTCT CCAAACCATT TCCAAACGCG AAAAATGTCA TGTGTCAGTT
    GTTGGAGAAG TAGAAAAAGA GCAAAGAGTC AAGCTCCTTG GGAAATCTGG TGAAATTGCA
    GTTGACCTAG ATACCCGGCA ACTTGGAGAA CGAGAAAAGA AGGTGTTCAA GTTGAAAAGT
    GCCCCGAGGG TTTTGAAGAA ACTAGAGTTA CCAGAAAATC TGACAGTAAG AAAAGCTCTG
    AAAAGAGTTT TGATGTTGCC TTCAGTTGCT AGCAAAAGAT ATTTGACATG CAAAGTAGAC
    CGATCTGTAA CTGGATTGGT TGCTCAACAG CAATGTGTTG GACCTCTACA CACCCCACTT
    GCTGATGTAG CAGTCGTGGC TCTGTCACAT TTTGATACGG TTGGTGGAGC CGTGTCTCTT
    GGCGAACAAC CCATAAAAAT GCTAATCGAC GCTGAAAAAG GAGCCCGAAT GTGTATTGCG
    GAGACGATAA TGAACCTCAT TTGGGCACCA ATTACCGATT TAAAAGACGT AAAAATGTCG
    GGTAACTGGA TGTGGGCTGC AAAATGTGAC GGAGAAGGGG CTCGTTTAGT TGATGCTGTT
    GGAGCTCTTT GTCGTGGACT TCGTGAGATT GGATGTGCGA TTGACGGAGG AAAAGATTCG
    TTATCAATGG CGGTGACCGC CCATGGAGAA GTGGTCAAGA GTCCCGGTAC TCTTGTACTT
    TCCGCATATG CTCCTTGCAC AAATGTTACA AAAGTTGTGA ATCCATCGCT GAAAGCTGTT
    CCCGGTTCAA AAATCTTGTG GATCAAGATT GGAAGTTCCG AAGAGAAAAT GAGGCTTGGA
    GGAAGTGCAC TGGCACAAGT TTATTCACAG ATTGGAGACG ACTGTCCGGA TATTGAGAAC
    TTCTCGGAAA TTTCTAAAGT GTTCTCAATT GTTCAACAGT TACTGAATAG AGAAGAGCTG
    GCAGGGCCTT TGAGAAAACC AATTATTTTG GCAGGACATG ACATATCGGA TGGAGGACTC
    TTGACTGCTA TTCTGGAAAT GGCATTTGCT GGAAATGTTT CTATTGATAT TGATATCAAG
    CCCCCCAACC AGAATATCAA GCCTATCGAT ATTTTATTCG CTGAAGAATG TGGCATACTT
    TTGGAAGTTT CTAATCCTGA AAATGTTCTG CACATATTCT CTGAAGCTGG AATTAAATGC
    CAAGAAATTG GAAAAGCATC TGCCGTGTTT GGTCCAGATG CCCACGTAAA AATCCATGTG
    AATGGTCATT TGGAAATCAA TGAGAAGCTG GTTGATTTGA GAGAAGAATG GGAATTGGTT
    GGAGATCGAT TGGGAGAGTT TCAGACAAAT CCCAAGAGTT TGAAGGAAGC AAGAGAGGTT
    CGAAGAACAT GTCAGAAGAT CAATTATAAA TGTGATTTTG ATTGGTATTA TAATCCAGCG
    TTTATTCATA ATGAGCAGTA CTTTTCTACA GCCCCACGAG TCGCTATAAT CCGTGAAGAA
    GGTTCGAATG GTGATCGAGA AATGGCTTCT GCATTCACTC TTGCTGGATT CCAAACATTC
    GATGTCACAA TGACTGATAT TTTGGCTGGG CACACTTTGG AAGCTTACAG AGGTGTCGCG
    TTTGTGGGTG GATTTTCATA TGCTGATGTT CTGGGGTCTG CGAAAGGTTG GGCTGCCGGT
    GTTCAATTCA ACGAGTCTGT TTCAAAGCAA TTCGAAGCTT TCCGTTCGAG ACCTGACACA
    TTCTCATATG GAGTTTGTAA TGGATGTCAG TTGATGGCTC AATTGGGATG GATTGGGGAT
    GAAGAACAGA AAGGGCCTAC TGTTTTTTTG GATGAAAACG AATGTGGGAG ATTTGATAGC
    AGTTTTGGGC CAGTTAAAAT CGAAAAAAAC GTCAGCATTA TGCTTTCCGG AATGGAAAAC
    TCCGTTCTCG GCCTCTGGTC TTCTCATGGA GAGGGTCGCT TCACCTACCG TAACCTTCAA
    AACTTCCAAA ATCTGAAGAC AAATGGTCAA GTCTGTATTC GTTTTTGTGA TGATCGAGGA
    ATGACTGGCG CTGATCATGG ATCCGTGAAG CTACCGTACC CATGGAATCC CAATGGATCC
    ATTGATGACG TGGCAGCTAT TTGTAGTCGG GACGGACGTC ATCTTGCTAT GATGCCACAT
    GCTGACAGAT CATTCTTGAC GTGGCAATGG GCGGAGTCAA GTGAGGTTCC ATGGAATGCA
    AGATTCGATC AAAAGACAGT GGCACTTTCC CCATGGATCA AAATGTTTAG AAATGCATAT
    AATTGGTGTC TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.