bpl-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	bpl-1
Entrez Gene ID	174039
Full Name	Biotin Protein Ligase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_062856.7	NP_495257.3	BPL/LPL catalytic domain-containing protein
NM_182120.5	NP_871920.1	BPL/LPL catalytic domain-containing protein
NM_062856.6	NP_495257.3	Biotin Protein Ligase
NM_182121.3	NP_871921.1	Biotin Protein Ligase
NM_182120.4	NP_871920.1	Biotin Protein Ligase
NM_001373729.1	NP_001359904.1	BPL/LPL catalytic domain-containing protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLCS	NP_000402.3
Arabidopsis thaliana (thale cress)	HCS1	NP_565605.1
Xenopus tropicalis (tropical clawed frog)	hlcs	XP_004912175.1
Pan troglodytes (chimpanzee)	HLCS	XP_003319106.2
	HLCS	XP_001084173.2
Bos taurus (cattle)	HLCS	XP_005202184.1
Arabidopsis thaliana (thale cress)	HCS2	NP_850957.1
Mus musculus (house mouse)	Hlcs	NP_631884.1
Gallus gallus (chicken)	HLCS	XP_004934596.1
Canis lupus familiaris (dog)	HLCS	XP_005638959.1
Rattus norvegicus (Norway rat)	Hlcs	XP_006248175.1
Caenorhabditis elegans (roundworm)	bpl-1	NP_871920.1
Danio rerio (zebrafish)	hlcs	XP_002663496.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bpl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bpl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338815	NM_182120.5 Latest version!	Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa338816	NM_001373729.1 Latest version!	Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa338816	NM_182121.3 Latest version!	Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa338817	NM_062856.7 Latest version!	Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa338817	NM_062856.6	Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa338815	NM_182120.4	Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bpl-1 ( NM_182120.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871920.1 Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.

Price & Availability↑

CloneID	OCa338815
Clone ID Related Accession (Same CDS sequence)	NM_182120.5 , NM_182120.4
Accession Version	NM_182120.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BPL/LPL catalytic domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_182120.4.

ATGAACTTTC CTAGAGTTAT TAGCAATGCC CGTCACATTG CTCTACTTTT TACAGGCGAT 
CGAGGAAGAT CTGTCAGTCC TTCATTTGAA TATTATCAAA ATCGATCTCT ACGCGGATTC 
ACTTCTGCAG CCAATCCACA TCGCGTCGCT ACTCGAAATT CCACAGTTCG TCAAGTACCA 
CTTTACCAGT TCCTGACATC AAGAAGTTCA TCATTCAAGG CAATGTGTAA ACCGAACTCT 
GTTCTCGTAT ATACCGGAGG GAATGATAGT CTTTATTCTG ATATCCGTCA TCGATTATCA 
TTTCTTTTAC CTCCAGATGA GATCACTGTT TTTAATGTGT CAATTCAAGC TTTGAAAAAA 
CAGCCATGGG CAGAAAAATC AACGGTTTGT GTAATACTTG CGTCTACGAA CGATTTGGAC 
GATGAAGCAT GGGAGAAAAT TCAAGCATAT TTCAATCAAA ACGGAAAAAT AATTTTTGTC 
TGCCAGAATA AGCTATTGGC AAGTATCACA GGTTGTGATT CGAGTAAAGC GAACGCTTCT 
ATTCTTCGAT TTGCATTTGG GAGTCAAAAT AACAAATTGA AAGATACAAA TAAGGAATTC 
GTGAAATTTC TTGAGAAGAA TATGAAAAAG CTTCCGAAAT CGACAGCAAT TAATGAGACT 
TTTCGCTCAA AAGATGTCAG TGTTGGAGCA AATTTCACAG TTGTACTCAA GAAAGAACCA 
GATGCACCCT TGTTCTTGTA TATGCAAAAT AACGGAAGCC TGCATGCTTC AGCATTGTTC 
TCTGATGCAA CAACTCAACA ATTGATAGCA CCAAATAGTA ATTTACTGAA AGATTCTCTA 
CGAAGCGTAG GAGTAAACGT CTGTGACACG ACAATGCCTC CATTAACCAA AGGAATTCTT 
ATTGCTGAAT ATGACTCAAT TATAGAAAGC ATCGCTGGCC TTCGATTGGG CGAAGAAATT 
GGTTTACAGC CGCGAATTCT GCTCAGGAAA AGTGATGTTG TAGAAGAATT AGGACTTCCT 
GAAGCTACTG AGAAGCTTCT TCCAATCGAG GTGGTTAACC GGGATTCTGA TGCTGGAACC 
AGTAAAAATT TTGACTTCAA TCTTTATTTC GAACAAATTC ATTCAAAAAT CGGTCAAGTC 
GTACTGCTTG TTGATGTAGC AACAACTACA ATGGATATTA TTGAAAGTGT GAATGCCGGA 
ATTCCATCAC TCGAAAGTGT AGTTGTAATT GCAAATCGTC AAATCAGCGG AAGAGGACGA 
GGTGGAAATG AGTTCCTTTG TCCACGTGGG ATGGCAATGT TCAACTTTTC TTTTTCTATC 
TCAAAAAAGA GCAGAATCGC AAAACATCTT CCAATTCTCC AGCACATTTT CTGTGTGGCA 
CTTGTTGAAG CAGCACGGAA TCTATCTGGA TATCCAGAGT TTCCACTGCA TATCAAATGG 
CCAAATGATC TGTATTGTGA ACGATCTCAT AAAGTTGGTG GAATGCTTCT TCAGTGCAGT 
ACTAGGGATG ATTCATTCCG AGTCAGTATC GGATGTGGAA TGAATGTTTC GAACGATAAA 
CCGACGATGT GCTTGAATGA TATGCTGCCT AAAGAAGCCG AAACACGGAT TACAAAAGAA 
CAATTGATTG CGGAAACAAT AAACAAATTC ACTTATTACA TGAAGGACTA TGAGGATAAT 
GGCCCGGAAA CCTTCAAAAA GAAGTATCAC GAGTACTGGC TGCATTCACA ACAAGAAGTT 
CTACTGTCAG ACTTCAATGA ACGAGTAACG ATTCGAGGAA TCGACGATGA CGGATACCTG 
CAAGTACGCA GCAAGTCCAA CCCGGATAAA ATATTCTCAA TCGGTGATGA TGGAAATACC 
TTCGACATGA TGAAGGGTCT AATTCGTCAT AAATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871920.1
CDS	38..1936
Translation	MNFPRVISNARHIALLFTGDRGRSVSPSFEYYQNRSLRGFTSAANPHRVATRNSTVRQVPLYQFLTSRSSSFKAMCKPNSVLVYTGGNDSLYSDIRHRLSFLLPPDEITVFNVSIQALKKQPWAEKSTVCVILASTNDLDDEAWEKIQAYFNQNGKIIFVCQNKLLASITGCDSSKANASILRFAFGSQNNKLKDTNKEFVKFLEKNMKKLPKSTAINETFRSKDVSVGANFTVVLKKEPDAPLFLYMQNNGSLHASALFSDATTQQLIAPNSNLLKDSLRSVGVNVCDTTMPPLTKGILIAEYDSIIESIAGLRLGEEIGLQPRILLRKSDVVEELGLPEATEKLLPIEVVNRDSDAGTSKNFDFNLYFEQIHSKIGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNLSGYPEFPLHIKWPNDLYCERSHKVGGMLLQCSTRDDSFRVSIGCGMNVSNDKPTMCLNDMLPKEAETRITKEQLIAETINKFTYYMKDYEDNGPETFKKKYHEYWLHSQQEVLLSDFNERVTIRGIDDDGYLQVRSKSNPDKIFSIGDDGNTFDMMKGLIRHKY

Target ORF information:

RefSeq Version	NM_182120.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182120.5

ORF Insert Sequence:

ATGAACTTTC CTAGAGTTAT TAGCAATGCC CGTCACATTG CTCTACTTTT TACAGGCGAT 
CGAGGAAGAT CTGTCAGTCC TTCATTTGAA TATTATCAAA ATCGATCTCT ACGCGGATTC 
ACTTCTGCAG CCAATCCACA TCGCGTCGCT ACTCGAAATT CCACAGTTCG TCAAGTACCA 
CTTTACCAGT TCCTGACATC AAGAAGTTCA TCATTCAAGG CAATGTGTAA ACCGAACTCT 
GTTCTCGTAT ATACCGGAGG GAATGATAGT CTTTATTCTG ATATCCGTCA TCGATTATCA 
TTTCTTTTAC CTCCAGATGA GATCACTGTT TTTAATGTGT CAATTCAAGC TTTGAAAAAA 
CAGCCATGGG CAGAAAAATC AACGGTTTGT GTAATACTTG CGTCTACGAA CGATTTGGAC 
GATGAAGCAT GGGAGAAAAT TCAAGCATAT TTCAATCAAA ACGGAAAAAT AATTTTTGTC 
TGCCAGAATA AGCTATTGGC AAGTATCACA GGTTGTGATT CGAGTAAAGC GAACGCTTCT 
ATTCTTCGAT TTGCATTTGG GAGTCAAAAT AACAAATTGA AAGATACAAA TAAGGAATTC 
GTGAAATTTC TTGAGAAGAA TATGAAAAAG CTTCCGAAAT CGACAGCAAT TAATGAGACT 
TTTCGCTCAA AAGATGTCAG TGTTGGAGCA AATTTCACAG TTGTACTCAA GAAAGAACCA 
GATGCACCCT TGTTCTTGTA TATGCAAAAT AACGGAAGCC TGCATGCTTC AGCATTGTTC 
TCTGATGCAA CAACTCAACA ATTGATAGCA CCAAATAGTA ATTTACTGAA AGATTCTCTA 
CGAAGCGTAG GAGTAAACGT CTGTGACACG ACAATGCCTC CATTAACCAA AGGAATTCTT 
ATTGCTGAAT ATGACTCAAT TATAGAAAGC ATCGCTGGCC TTCGATTGGG CGAAGAAATT 
GGTTTACAGC CGCGAATTCT GCTCAGGAAA AGTGATGTTG TAGAAGAATT AGGACTTCCT 
GAAGCTACTG AGAAGCTTCT TCCAATCGAG GTGGTTAACC GGGATTCTGA TGCTGGAACC 
AGTAAAAATT TTGACTTCAA TCTTTATTTC GAACAAATTC ATTCAAAAAT CGGTCAAGTC 
GTACTGCTTG TTGATGTAGC AACAACTACA ATGGATATTA TTGAAAGTGT GAATGCCGGA 
ATTCCATCAC TCGAAAGTGT AGTTGTAATT GCAAATCGTC AAATCAGCGG AAGAGGACGA 
GGTGGAAATG AGTTCCTTTG TCCACGTGGG ATGGCAATGT TCAACTTTTC TTTTTCTATC 
TCAAAAAAGA GCAGAATCGC AAAACATCTT CCAATTCTCC AGCACATTTT CTGTGTGGCA 
CTTGTTGAAG CAGCACGGAA TCTATCTGGA TATCCAGAGT TTCCACTGCA TATCAAATGG 
CCAAATGATC TGTATTGTGA ACGATCTCAT AAAGTTGGTG GAATGCTTCT TCAGTGCAGT 
ACTAGGGATG ATTCATTCCG AGTCAGTATC GGATGTGGAA TGAATGTTTC GAACGATAAA 
CCGACGATGT GCTTGAATGA TATGCTGCCT AAAGAAGCCG AAACACGGAT TACAAAAGAA 
CAATTGATTG CGGAAACAAT AAACAAATTC ACTTATTACA TGAAGGACTA TGAGGATAAT 
GGCCCGGAAA CCTTCAAAAA GAAGTATCAC GAGTACTGGC TGCATTCACA ACAAGAAGTT 
CTACTGTCAG ACTTCAATGA ACGAGTAACG ATTCGAGGAA TCGACGATGA CGGATACCTG 
CAAGTACGCA GCAAGTCCAA CCCGGATAAA ATATTCTCAA TCGGTGATGA TGGAAATACC 
TTCGACATGA TGAAGGGTCT AATTCGTCAT AAATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bpl-1 ( NM_182120.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871920.1 Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.

Price & Availability↑

CloneID	OCa338815
Clone ID Related Accession (Same CDS sequence)	NM_182120.5 , NM_182120.4
Accession Version	NM_182120.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Biotin Protein Ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_182120.3. COMPLETENESS: incomplete on both ends.

ATGAACTTTC CTAGAGTTAT TAGCAATGCC CGTCACATTG CTCTACTTTT TACAGGCGAT 
CGAGGAAGAT CTGTCAGTCC TTCATTTGAA TATTATCAAA ATCGATCTCT ACGCGGATTC 
ACTTCTGCAG CCAATCCACA TCGCGTCGCT ACTCGAAATT CCACAGTTCG TCAAGTACCA 
CTTTACCAGT TCCTGACATC AAGAAGTTCA TCATTCAAGG CAATGTGTAA ACCGAACTCT 
GTTCTCGTAT ATACCGGAGG GAATGATAGT CTTTATTCTG ATATCCGTCA TCGATTATCA 
TTTCTTTTAC CTCCAGATGA GATCACTGTT TTTAATGTGT CAATTCAAGC TTTGAAAAAA 
CAGCCATGGG CAGAAAAATC AACGGTTTGT GTAATACTTG CGTCTACGAA CGATTTGGAC 
GATGAAGCAT GGGAGAAAAT TCAAGCATAT TTCAATCAAA ACGGAAAAAT AATTTTTGTC 
TGCCAGAATA AGCTATTGGC AAGTATCACA GGTTGTGATT CGAGTAAAGC GAACGCTTCT 
ATTCTTCGAT TTGCATTTGG GAGTCAAAAT AACAAATTGA AAGATACAAA TAAGGAATTC 
GTGAAATTTC TTGAGAAGAA TATGAAAAAG CTTCCGAAAT CGACAGCAAT TAATGAGACT 
TTTCGCTCAA AAGATGTCAG TGTTGGAGCA AATTTCACAG TTGTACTCAA GAAAGAACCA 
GATGCACCCT TGTTCTTGTA TATGCAAAAT AACGGAAGCC TGCATGCTTC AGCATTGTTC 
TCTGATGCAA CAACTCAACA ATTGATAGCA CCAAATAGTA ATTTACTGAA AGATTCTCTA 
CGAAGCGTAG GAGTAAACGT CTGTGACACG ACAATGCCTC CATTAACCAA AGGAATTCTT 
ATTGCTGAAT ATGACTCAAT TATAGAAAGC ATCGCTGGCC TTCGATTGGG CGAAGAAATT 
GGTTTACAGC CGCGAATTCT GCTCAGGAAA AGTGATGTTG TAGAAGAATT AGGACTTCCT 
GAAGCTACTG AGAAGCTTCT TCCAATCGAG GTGGTTAACC GGGATTCTGA TGCTGGAACC 
AGTAAAAATT TTGACTTCAA TCTTTATTTC GAACAAATTC ATTCAAAAAT CGGTCAAGTC 
GTACTGCTTG TTGATGTAGC AACAACTACA ATGGATATTA TTGAAAGTGT GAATGCCGGA 
ATTCCATCAC TCGAAAGTGT AGTTGTAATT GCAAATCGTC AAATCAGCGG AAGAGGACGA 
GGTGGAAATG AGTTCCTTTG TCCACGTGGG ATGGCAATGT TCAACTTTTC TTTTTCTATC 
TCAAAAAAGA GCAGAATCGC AAAACATCTT CCAATTCTCC AGCACATTTT CTGTGTGGCA 
CTTGTTGAAG CAGCACGGAA TCTATCTGGA TATCCAGAGT TTCCACTGCA TATCAAATGG 
CCAAATGATC TGTATTGTGA ACGATCTCAT AAAGTTGGTG GAATGCTTCT TCAGTGCAGT 
ACTAGGGATG ATTCATTCCG AGTCAGTATC GGATGTGGAA TGAATGTTTC GAACGATAAA 
CCGACGATGT GCTTGAATGA TATGCTGCCT AAAGAAGCCG AAACACGGAT TACAAAAGAA 
CAATTGATTG CGGAAACAAT AAACAAATTC ACTTATTACA TGAAGGACTA TGAGGATAAT 
GGCCCGGAAA CCTTCAAAAA GAAGTATCAC GAGTACTGGC TGCATTCACA ACAAGAAGTT 
CTACTGTCAG ACTTCAATGA ACGAGTAACG ATTCGAGGAA TCGACGATGA CGGATACCTG 
CAAGTACGCA GCAAGTCCAA CCCGGATAAA ATATTCTCAA TCGGTGATGA TGGAAATACC 
TTCGACATGA TGAAGGGTCT AATTCGTCAT AAATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871920.1
CDS	1..1899
Translation	MNFPRVISNARHIALLFTGDRGRSVSPSFEYYQNRSLRGFTSAANPHRVATRNSTVRQVPLYQFLTSRSSSFKAMCKPNSVLVYTGGNDSLYSDIRHRLSFLLPPDEITVFNVSIQALKKQPWAEKSTVCVILASTNDLDDEAWEKIQAYFNQNGKIIFVCQNKLLASITGCDSSKANASILRFAFGSQNNKLKDTNKEFVKFLEKNMKKLPKSTAINETFRSKDVSVGANFTVVLKKEPDAPLFLYMQNNGSLHASALFSDATTQQLIAPNSNLLKDSLRSVGVNVCDTTMPPLTKGILIAEYDSIIESIAGLRLGEEIGLQPRILLRKSDVVEELGLPEATEKLLPIEVVNRDSDAGTSKNFDFNLYFEQIHSKIGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNLSGYPEFPLHIKWPNDLYCERSHKVGGMLLQCSTRDDSFRVSIGCGMNVSNDKPTMCLNDMLPKEAETRITKEQLIAETINKFTYYMKDYEDNGPETFKKKYHEYWLHSQQEVLLSDFNERVTIRGIDDDGYLQVRSKSNPDKIFSIGDDGNTFDMMKGLIRHKY

Target ORF information:

RefSeq Version	NM_182120.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182120.4

ORF Insert Sequence:

ATGAACTTTC CTAGAGTTAT TAGCAATGCC CGTCACATTG CTCTACTTTT TACAGGCGAT 
CGAGGAAGAT CTGTCAGTCC TTCATTTGAA TATTATCAAA ATCGATCTCT ACGCGGATTC 
ACTTCTGCAG CCAATCCACA TCGCGTCGCT ACTCGAAATT CCACAGTTCG TCAAGTACCA 
CTTTACCAGT TCCTGACATC AAGAAGTTCA TCATTCAAGG CAATGTGTAA ACCGAACTCT 
GTTCTCGTAT ATACCGGAGG GAATGATAGT CTTTATTCTG ATATCCGTCA TCGATTATCA 
TTTCTTTTAC CTCCAGATGA GATCACTGTT TTTAATGTGT CAATTCAAGC TTTGAAAAAA 
CAGCCATGGG CAGAAAAATC AACGGTTTGT GTAATACTTG CGTCTACGAA CGATTTGGAC 
GATGAAGCAT GGGAGAAAAT TCAAGCATAT TTCAATCAAA ACGGAAAAAT AATTTTTGTC 
TGCCAGAATA AGCTATTGGC AAGTATCACA GGTTGTGATT CGAGTAAAGC GAACGCTTCT 
ATTCTTCGAT TTGCATTTGG GAGTCAAAAT AACAAATTGA AAGATACAAA TAAGGAATTC 
GTGAAATTTC TTGAGAAGAA TATGAAAAAG CTTCCGAAAT CGACAGCAAT TAATGAGACT 
TTTCGCTCAA AAGATGTCAG TGTTGGAGCA AATTTCACAG TTGTACTCAA GAAAGAACCA 
GATGCACCCT TGTTCTTGTA TATGCAAAAT AACGGAAGCC TGCATGCTTC AGCATTGTTC 
TCTGATGCAA CAACTCAACA ATTGATAGCA CCAAATAGTA ATTTACTGAA AGATTCTCTA 
CGAAGCGTAG GAGTAAACGT CTGTGACACG ACAATGCCTC CATTAACCAA AGGAATTCTT 
ATTGCTGAAT ATGACTCAAT TATAGAAAGC ATCGCTGGCC TTCGATTGGG CGAAGAAATT 
GGTTTACAGC CGCGAATTCT GCTCAGGAAA AGTGATGTTG TAGAAGAATT AGGACTTCCT 
GAAGCTACTG AGAAGCTTCT TCCAATCGAG GTGGTTAACC GGGATTCTGA TGCTGGAACC 
AGTAAAAATT TTGACTTCAA TCTTTATTTC GAACAAATTC ATTCAAAAAT CGGTCAAGTC 
GTACTGCTTG TTGATGTAGC AACAACTACA ATGGATATTA TTGAAAGTGT GAATGCCGGA 
ATTCCATCAC TCGAAAGTGT AGTTGTAATT GCAAATCGTC AAATCAGCGG AAGAGGACGA 
GGTGGAAATG AGTTCCTTTG TCCACGTGGG ATGGCAATGT TCAACTTTTC TTTTTCTATC 
TCAAAAAAGA GCAGAATCGC AAAACATCTT CCAATTCTCC AGCACATTTT CTGTGTGGCA 
CTTGTTGAAG CAGCACGGAA TCTATCTGGA TATCCAGAGT TTCCACTGCA TATCAAATGG 
CCAAATGATC TGTATTGTGA ACGATCTCAT AAAGTTGGTG GAATGCTTCT TCAGTGCAGT 
ACTAGGGATG ATTCATTCCG AGTCAGTATC GGATGTGGAA TGAATGTTTC GAACGATAAA 
CCGACGATGT GCTTGAATGA TATGCTGCCT AAAGAAGCCG AAACACGGAT TACAAAAGAA 
CAATTGATTG CGGAAACAAT AAACAAATTC ACTTATTACA TGAAGGACTA TGAGGATAAT 
GGCCCGGAAA CCTTCAAAAA GAAGTATCAC GAGTACTGGC TGCATTCACA ACAAGAAGTT 
CTACTGTCAG ACTTCAATGA ACGAGTAACG ATTCGAGGAA TCGACGATGA CGGATACCTG 
CAAGTACGCA GCAAGTCCAA CCCGGATAAA ATATTCTCAA TCGGTGATGA TGGAAATACC 
TTCGACATGA TGAAGGGTCT AATTCGTCAT AAATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bpl-1 ( NM_182121.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871921.1 Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.

Price & Availability↑

CloneID	OCa338816
Clone ID Related Accession (Same CDS sequence)	NM_182121.3 , NM_001373729.1
Accession Version	NM_182121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Biotin Protein Ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_182121.2. COMPLETENESS: incomplete on both ends.

ATGTGTAAAC CGAACTCTGT TCTCGTATAT ACCGGAGGGA ATGATAGTCT TTATTCTGAT 
ATCCGTCATC GATTATCATT TCTTTTACCT CCAGATGAGA TCACTGTTTT TAATGTGTCA 
ATTCAAGCTT TGAAAAAACA GCCATGGGCA GAAAAATCAA CGGTTTGTGT AATACTTGCG 
TCTACGAACG ATTTGGACGA TGAAGCATGG GAGAAAATTC AAGCATATTT CAATCAAAAC 
GGAAAAATAA TTTTTGTCTG CCAGAATAAG CTATTGGCAA GTATCACAGG TTGTGATTCG 
AGTAAAGCGA ACGCTTCTAT TCTTCGATTT GCATTTGGGA GTCAAAATAA CAAATTGAAA 
GATACAAATA AGGAATTCGT GAAATTTCTT GAGAAGAATA TGAAAAAGCT TCCGAAATCG 
ACAGCAATTA ATGAGACTTT TCGCTCAAAA GATGTCAGTG TTGGAGCAAA TTTCACAGTT 
GTACTCAAGA AAGAACCAGA TGCACCCTTG TTCTTGTATA TGCAAAATAA CGGAAGCCTG 
CATGCTTCAG CATTGTTCTC TGATGCAACA ACTCAACAAT TGATAGCACC AAATAGTAAT 
TTACTGAAAG ATTCTCTACG AAGCGTAGGA GTAAACGTCT GTGACACGAC AATGCCTCCA 
TTAACCAAAG GAATTCTTAT TGCTGAATAT GACTCAATTA TAGAAAGCAT CGCTGGCCTT 
CGATTGGGCG AAGAAATTGG TTTACAGCCG CGAATTCTGC TCAGGAAAAG TGATGTTGTA 
GAAGAATTAG GACTTCCTGA AGCTACTGAG AAGCTTCTTC CAATCGAGGT GGTTAACCGG 
GATTCTGATG CTGGAACCAG TAAAAATTTT GACTTCAATC TTTATTTCGA ACAAATTCAT 
TCAAAAATCG GTCAAGTCGT ACTGCTTGTT GATGTAGCAA CAACTACAAT GGATATTATT 
GAAAGTGTGA ATGCCGGAAT TCCATCACTC GAAAGTGTAG TTGTAATTGC AAATCGTCAA 
ATCAGCGGAA GAGGACGAGG TGGAAATGAG TTCCTTTGTC CACGTGGGAT GGCAATGTTC 
AACTTTTCTT TTTCTATCTC AAAAAAGAGC AGAATCGCAA AACATCTTCC AATTCTCCAG 
CACATTTTCT GTGTGGCACT TGTTGAAGCA GCACGGAATC TATCTGGATA TCCAGAGTTT 
CCACTGCATA TCAAATGGCC AAATGATCTG TATTGTGAAC GATCTCATAA AGTTGGTGGA 
ATGCTTCTTC AGTGCAGTAC TAGGGATGAT TCATTCCGAG TCAGTATCGG ATGTGGAATG 
AATGTTTCGA ACGATAAACC GACGATGTGC TTGAATGATA TGCTGCCTAA AGAAGCCGAA 
ACACGGATTA CAAAAGAACA ATTGATTGCG GAAACAATAA ACAAATTCAC TTATTACATG 
AAGGACTATG AGGATAATGG CCCGGAAACC TTCAAAAAGA AGTATCACGA GTACTGGCTG 
CATTCACAAC AAGAAGTTCT ACTGTCAGAC TTCAATGAAC GAGTAACGAT TCGAGGAATC 
GACGATGACG GATACCTGCA AGTACGCAGC AAGTCCAACC CGGATAAAAT ATTCTCAATC 
GGTGATGATG GAAATACCTT CGACATGATG AAGGGTCTAA TTCGTCATAA ATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871921.1
CDS	1..1677
Translation	MCKPNSVLVYTGGNDSLYSDIRHRLSFLLPPDEITVFNVSIQALKKQPWAEKSTVCVILASTNDLDDEAWEKIQAYFNQNGKIIFVCQNKLLASITGCDSSKANASILRFAFGSQNNKLKDTNKEFVKFLEKNMKKLPKSTAINETFRSKDVSVGANFTVVLKKEPDAPLFLYMQNNGSLHASALFSDATTQQLIAPNSNLLKDSLRSVGVNVCDTTMPPLTKGILIAEYDSIIESIAGLRLGEEIGLQPRILLRKSDVVEELGLPEATEKLLPIEVVNRDSDAGTSKNFDFNLYFEQIHSKIGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNLSGYPEFPLHIKWPNDLYCERSHKVGGMLLQCSTRDDSFRVSIGCGMNVSNDKPTMCLNDMLPKEAETRITKEQLIAETINKFTYYMKDYEDNGPETFKKKYHEYWLHSQQEVLLSDFNERVTIRGIDDDGYLQVRSKSNPDKIFSIGDDGNTFDMMKGLIRHKY

Target ORF information:

RefSeq Version	NM_182121.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182121.3

ORF Insert Sequence:

ATGTGTAAAC CGAACTCTGT TCTCGTATAT ACCGGAGGGA ATGATAGTCT TTATTCTGAT 
ATCCGTCATC GATTATCATT TCTTTTACCT CCAGATGAGA TCACTGTTTT TAATGTGTCA 
ATTCAAGCTT TGAAAAAACA GCCATGGGCA GAAAAATCAA CGGTTTGTGT AATACTTGCG 
TCTACGAACG ATTTGGACGA TGAAGCATGG GAGAAAATTC AAGCATATTT CAATCAAAAC 
GGAAAAATAA TTTTTGTCTG CCAGAATAAG CTATTGGCAA GTATCACAGG TTGTGATTCG 
AGTAAAGCGA ACGCTTCTAT TCTTCGATTT GCATTTGGGA GTCAAAATAA CAAATTGAAA 
GATACAAATA AGGAATTCGT GAAATTTCTT GAGAAGAATA TGAAAAAGCT TCCGAAATCG 
ACAGCAATTA ATGAGACTTT TCGCTCAAAA GATGTCAGTG TTGGAGCAAA TTTCACAGTT 
GTACTCAAGA AAGAACCAGA TGCACCCTTG TTCTTGTATA TGCAAAATAA CGGAAGCCTG 
CATGCTTCAG CATTGTTCTC TGATGCAACA ACTCAACAAT TGATAGCACC AAATAGTAAT 
TTACTGAAAG ATTCTCTACG AAGCGTAGGA GTAAACGTCT GTGACACGAC AATGCCTCCA 
TTAACCAAAG GAATTCTTAT TGCTGAATAT GACTCAATTA TAGAAAGCAT CGCTGGCCTT 
CGATTGGGCG AAGAAATTGG TTTACAGCCG CGAATTCTGC TCAGGAAAAG TGATGTTGTA 
GAAGAATTAG GACTTCCTGA AGCTACTGAG AAGCTTCTTC CAATCGAGGT GGTTAACCGG 
GATTCTGATG CTGGAACCAG TAAAAATTTT GACTTCAATC TTTATTTCGA ACAAATTCAT 
TCAAAAATCG GTCAAGTCGT ACTGCTTGTT GATGTAGCAA CAACTACAAT GGATATTATT 
GAAAGTGTGA ATGCCGGAAT TCCATCACTC GAAAGTGTAG TTGTAATTGC AAATCGTCAA 
ATCAGCGGAA GAGGACGAGG TGGAAATGAG TTCCTTTGTC CACGTGGGAT GGCAATGTTC 
AACTTTTCTT TTTCTATCTC AAAAAAGAGC AGAATCGCAA AACATCTTCC AATTCTCCAG 
CACATTTTCT GTGTGGCACT TGTTGAAGCA GCACGGAATC TATCTGGATA TCCAGAGTTT 
CCACTGCATA TCAAATGGCC AAATGATCTG TATTGTGAAC GATCTCATAA AGTTGGTGGA 
ATGCTTCTTC AGTGCAGTAC TAGGGATGAT TCATTCCGAG TCAGTATCGG ATGTGGAATG 
AATGTTTCGA ACGATAAACC GACGATGTGC TTGAATGATA TGCTGCCTAA AGAAGCCGAA 
ACACGGATTA CAAAAGAACA ATTGATTGCG GAAACAATAA ACAAATTCAC TTATTACATG 
AAGGACTATG AGGATAATGG CCCGGAAACC TTCAAAAAGA AGTATCACGA GTACTGGCTG 
CATTCACAAC AAGAAGTTCT ACTGTCAGAC TTCAATGAAC GAGTAACGAT TCGAGGAATC 
GACGATGACG GATACCTGCA AGTACGCAGC AAGTCCAACC CGGATAAAAT ATTCTCAATC 
GGTGATGATG GAAATACCTT CGACATGATG AAGGGTCTAA TTCGTCATAA ATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bpl-1 ( NM_001373729.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001359904.1 Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.

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CloneID	OCa338816
Clone ID Related Accession (Same CDS sequence)	NM_182121.3 , NM_001373729.1
Accession Version	NM_001373729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BPL/LPL catalytic domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280).

ATGTGTAAAC CGAACTCTGT TCTCGTATAT ACCGGAGGGA ATGATAGTCT TTATTCTGAT 
ATCCGTCATC GATTATCATT TCTTTTACCT CCAGATGAGA TCACTGTTTT TAATGTGTCA 
ATTCAAGCTT TGAAAAAACA GCCATGGGCA GAAAAATCAA CGGTTTGTGT AATACTTGCG 
TCTACGAACG ATTTGGACGA TGAAGCATGG GAGAAAATTC AAGCATATTT CAATCAAAAC 
GGAAAAATAA TTTTTGTCTG CCAGAATAAG CTATTGGCAA GTATCACAGG TTGTGATTCG 
AGTAAAGCGA ACGCTTCTAT TCTTCGATTT GCATTTGGGA GTCAAAATAA CAAATTGAAA 
GATACAAATA AGGAATTCGT GAAATTTCTT GAGAAGAATA TGAAAAAGCT TCCGAAATCG 
ACAGCAATTA ATGAGACTTT TCGCTCAAAA GATGTCAGTG TTGGAGCAAA TTTCACAGTT 
GTACTCAAGA AAGAACCAGA TGCACCCTTG TTCTTGTATA TGCAAAATAA CGGAAGCCTG 
CATGCTTCAG CATTGTTCTC TGATGCAACA ACTCAACAAT TGATAGCACC AAATAGTAAT 
TTACTGAAAG ATTCTCTACG AAGCGTAGGA GTAAACGTCT GTGACACGAC AATGCCTCCA 
TTAACCAAAG GAATTCTTAT TGCTGAATAT GACTCAATTA TAGAAAGCAT CGCTGGCCTT 
CGATTGGGCG AAGAAATTGG TTTACAGCCG CGAATTCTGC TCAGGAAAAG TGATGTTGTA 
GAAGAATTAG GACTTCCTGA AGCTACTGAG AAGCTTCTTC CAATCGAGGT GGTTAACCGG 
GATTCTGATG CTGGAACCAG TAAAAATTTT GACTTCAATC TTTATTTCGA ACAAATTCAT 
TCAAAAATCG GTCAAGTCGT ACTGCTTGTT GATGTAGCAA CAACTACAAT GGATATTATT 
GAAAGTGTGA ATGCCGGAAT TCCATCACTC GAAAGTGTAG TTGTAATTGC AAATCGTCAA 
ATCAGCGGAA GAGGACGAGG TGGAAATGAG TTCCTTTGTC CACGTGGGAT GGCAATGTTC 
AACTTTTCTT TTTCTATCTC AAAAAAGAGC AGAATCGCAA AACATCTTCC AATTCTCCAG 
CACATTTTCT GTGTGGCACT TGTTGAAGCA GCACGGAATC TATCTGGATA TCCAGAGTTT 
CCACTGCATA TCAAATGGCC AAATGATCTG TATTGTGAAC GATCTCATAA AGTTGGTGGA 
ATGCTTCTTC AGTGCAGTAC TAGGGATGAT TCATTCCGAG TCAGTATCGG ATGTGGAATG 
AATGTTTCGA ACGATAAACC GACGATGTGC TTGAATGATA TGCTGCCTAA AGAAGCCGAA 
ACACGGATTA CAAAAGAACA ATTGATTGCG GAAACAATAA ACAAATTCAC TTATTACATG 
AAGGACTATG AGGATAATGG CCCGGAAACC TTCAAAAAGA AGTATCACGA GTACTGGCTG 
CATTCACAAC AAGAAGTTCT ACTGTCAGAC TTCAATGAAC GAGTAACGAT TCGAGGAATC 
GACGATGACG GATACCTGCA AGTACGCAGC AAGTCCAACC CGGATAAAAT ATTCTCAATC 
GGTGATGATG GAAATACCTT CGACATGATG AAGGGTCTAA TTCGTCATAA ATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001359904.1
CDS	168..1844
Translation	MCKPNSVLVYTGGNDSLYSDIRHRLSFLLPPDEITVFNVSIQALKKQPWAEKSTVCVILASTNDLDDEAWEKIQAYFNQNGKIIFVCQNKLLASITGCDSSKANASILRFAFGSQNNKLKDTNKEFVKFLEKNMKKLPKSTAINETFRSKDVSVGANFTVVLKKEPDAPLFLYMQNNGSLHASALFSDATTQQLIAPNSNLLKDSLRSVGVNVCDTTMPPLTKGILIAEYDSIIESIAGLRLGEEIGLQPRILLRKSDVVEELGLPEATEKLLPIEVVNRDSDAGTSKNFDFNLYFEQIHSKIGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNLSGYPEFPLHIKWPNDLYCERSHKVGGMLLQCSTRDDSFRVSIGCGMNVSNDKPTMCLNDMLPKEAETRITKEQLIAETINKFTYYMKDYEDNGPETFKKKYHEYWLHSQQEVLLSDFNERVTIRGIDDDGYLQVRSKSNPDKIFSIGDDGNTFDMMKGLIRHKY

Target ORF information:

RefSeq Version	NM_001373729.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373729.1

ORF Insert Sequence:

ATGTGTAAAC CGAACTCTGT TCTCGTATAT ACCGGAGGGA ATGATAGTCT TTATTCTGAT 
ATCCGTCATC GATTATCATT TCTTTTACCT CCAGATGAGA TCACTGTTTT TAATGTGTCA 
ATTCAAGCTT TGAAAAAACA GCCATGGGCA GAAAAATCAA CGGTTTGTGT AATACTTGCG 
TCTACGAACG ATTTGGACGA TGAAGCATGG GAGAAAATTC AAGCATATTT CAATCAAAAC 
GGAAAAATAA TTTTTGTCTG CCAGAATAAG CTATTGGCAA GTATCACAGG TTGTGATTCG 
AGTAAAGCGA ACGCTTCTAT TCTTCGATTT GCATTTGGGA GTCAAAATAA CAAATTGAAA 
GATACAAATA AGGAATTCGT GAAATTTCTT GAGAAGAATA TGAAAAAGCT TCCGAAATCG 
ACAGCAATTA ATGAGACTTT TCGCTCAAAA GATGTCAGTG TTGGAGCAAA TTTCACAGTT 
GTACTCAAGA AAGAACCAGA TGCACCCTTG TTCTTGTATA TGCAAAATAA CGGAAGCCTG 
CATGCTTCAG CATTGTTCTC TGATGCAACA ACTCAACAAT TGATAGCACC AAATAGTAAT 
TTACTGAAAG ATTCTCTACG AAGCGTAGGA GTAAACGTCT GTGACACGAC AATGCCTCCA 
TTAACCAAAG GAATTCTTAT TGCTGAATAT GACTCAATTA TAGAAAGCAT CGCTGGCCTT 
CGATTGGGCG AAGAAATTGG TTTACAGCCG CGAATTCTGC TCAGGAAAAG TGATGTTGTA 
GAAGAATTAG GACTTCCTGA AGCTACTGAG AAGCTTCTTC CAATCGAGGT GGTTAACCGG 
GATTCTGATG CTGGAACCAG TAAAAATTTT GACTTCAATC TTTATTTCGA ACAAATTCAT 
TCAAAAATCG GTCAAGTCGT ACTGCTTGTT GATGTAGCAA CAACTACAAT GGATATTATT 
GAAAGTGTGA ATGCCGGAAT TCCATCACTC GAAAGTGTAG TTGTAATTGC AAATCGTCAA 
ATCAGCGGAA GAGGACGAGG TGGAAATGAG TTCCTTTGTC CACGTGGGAT GGCAATGTTC 
AACTTTTCTT TTTCTATCTC AAAAAAGAGC AGAATCGCAA AACATCTTCC AATTCTCCAG 
CACATTTTCT GTGTGGCACT TGTTGAAGCA GCACGGAATC TATCTGGATA TCCAGAGTTT 
CCACTGCATA TCAAATGGCC AAATGATCTG TATTGTGAAC GATCTCATAA AGTTGGTGGA 
ATGCTTCTTC AGTGCAGTAC TAGGGATGAT TCATTCCGAG TCAGTATCGG ATGTGGAATG 
AATGTTTCGA ACGATAAACC GACGATGTGC TTGAATGATA TGCTGCCTAA AGAAGCCGAA 
ACACGGATTA CAAAAGAACA ATTGATTGCG GAAACAATAA ACAAATTCAC TTATTACATG 
AAGGACTATG AGGATAATGG CCCGGAAACC TTCAAAAAGA AGTATCACGA GTACTGGCTG 
CATTCACAAC AAGAAGTTCT ACTGTCAGAC TTCAATGAAC GAGTAACGAT TCGAGGAATC 
GACGATGACG GATACCTGCA AGTACGCAGC AAGTCCAACC CGGATAAAAT ATTCTCAATC 
GGTGATGATG GAAATACCTT CGACATGATG AAGGGTCTAA TTCGTCATAA ATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bpl-1 ( NM_062856.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495257.3 Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.

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CloneID	OCa338817
Clone ID Related Accession (Same CDS sequence)	NM_062856.7 , NM_062856.6
Accession Version	NM_062856.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BPL/LPL catalytic domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_062856.6.

ATGATACTTT TCGTTTGGTC ACTAGTTTCA TCGGCCATTG AGGCTGCTCG AAAACGTCAT 
ATTGCTAATT TCCTTCGTGC CTTACTTGCT CAACAACGGG TTTCTAGAAA CTATGAAATG 
CTCATATGGA AAACAATTAT TAAATCTTCA CAATATTTAT CAACTCAAAG AGCCAGTTCA 
TCGAGATTGT ACCGACCAAC TAGTGAACCA AAACTCGATT CGACACTTGT CTTGCAGACT 
GATTTAATAC AAGTTTATGC CGAAAAATTC GAAACGCTTC GTGCCAGCGA ATGGAGATTT 
TGTCCGTCTT TATGGCCTTG TGAAGAGGAT TCAATATACT TTGTAGTACA TGATGAATTC 
ATGGAAAAAC GACAAAAAGA GCCAGGAGGA GATGAAAAAT TGGAACCAAA CTATTTTAAA 
CTTCACGAAA AGTGTGATCG ATTGGCAGTT CACAAAACAA TGACCCGATG CTTATATGCT 
TGCTCATTCG ATATGTTCAT AGATGTTGTA GATGCATTTT CTGAAAAGTT TCTCATTGAC 
GAAAACTTTC AAGTTACCAA AATTTTCAGC ATGTTTGCAC CGCCACAAGA ATTAGATCGA 
TCGTCAACGT CATCTCTCCG ACCCGAAATG CGAACTTCAA CGTTATCACT TGCATCGAAT 
TCTCTCCTTC CGAGACCTCT TCCAATTTTA CAGCATTTGT TCTGGGGAGA TCGTACAGGA 
TCAACGAAAT TCTCGACTAA TGATCGATAT GGAAGTGAAG GAAATTTAGA CATGTCCCCG 
AGTCGACGAC ATCCAGGCCG TCCATCATCA TCTGCAACAA TATCCAATTC AGCCAGAAGA 
CGTCGTTATC GTTCTTGTAA TTCACCGGAA CATCTTGAAT CTGAATCCAG TCAATTGACT 
CTTGAAAGCG GAAGTGAAGA ATTCCGAAGT CTTCCTCGAT ATATCAGACA ACTAAATCAA 
CGGGATTTTG GAAGCCCTAG TAATAGTATT ACGAACGGAT ATATTCCTGA TTCCGTCGAC 
TTCAATAGTT TGCCCAGATA TATTCGTCAT CTGGAAAAGT CGGTTTGCGA CTCAACAGAA 
GACGTTACTT CTTTTCGATC GGATAGAGCA CTTCCATCGT CTTCTTCCTG TAGTCATCCT 
CCAACAACTT CTGATTCTTG TACAAGTAGT CTAGCAGAAG AAGCAGCATC GAACAGCGGA 
ATATATAAGA ATCAAAGTTC AGCATCAAGA ACTAATTCAA ACGGATCATC AACTGATCGA 
AGGACACCTG ATTGTCATCC AATAAATTCT GGATTAAAAA GTCATTTCAC AATGGATGAA 
CTACGTATGA TTGATGAAAC CGATTCGGAG ACTGTCAATA CAGCAAGGGA ATTAATTGAA 
GAGGATGCTG AAAACATTTC ATTTGCAAAA CTTGCTTATC AAAATACGAA CGGAAATGCG 
GCAAATAAAC CTGAGAAGGA AAATAGAAAT GGCTCCAAAT TTGCAAATGG AAGTGTTACC 
ATCAAACTGG AAACAAGTGA TAATCTAAAT GACAAGATCA CGAATTCCGA TAGAACACCA 
ACACCGTATC CCCCAGCTGA TTCTGAAAGA CCAATAAGTG AAGCTTCCTC GTGTCATTCT 
GAATCTCCTC CGCGAGACTC GAAGCCTCCA CGAAGTCCAT CTAGAGTTAC GATTCCAACA 
ATCGACTTTT CTCGACTTCC TCCACTTGCT CAACAAACTG CCCGTTCTAA ACGAAGTACC 
TCTGTCGTCG CCAACAATAA CCGGCAAAAT TCCCAGCGTC GTTTCTCAGC TTTCTCAACG 
GAAAGCGATC GAGGAAGATC TGTCAGTCCT TCATTTGAAT ATTATCAAAA TCGATCTCTA 
CGCGGATTCA CTTCTGCAGC CAATCCACAT CGCGTCGCTA CTCGAAATTC CACAGTTCGT 
CAAGTACCAC TTTACCAGTT CCTGACATCA AGAAGTTCAT CATTCAAGGC AATGTGTAAA 
CCGAACTCTG TTCTCGTATA TACCGGAGGG AATGATAGTC TTTATTCTGA TATCCGTCAT 
CGATTATCAT TTCTTTTACC TCCAGATGAG ATCACTGTTT TTAATGTGTC AATTCAAGCT 
TTGAAAAAAC AGCCATGGGC AGAAAAATCA ACGGTTTGTG TAATACTTGC GTCTACGAAC 
GATTTGGACG ATGAAGCATG GGAGAAAATT CAAGCATATT TCAATCAAAA CGGAAAAATA 
ATTTTTGTCT GCCAGAATAA GCTATTGGCA AGTATCACAG GTTGTGATTC GAGTAAAGCG 
AACGCTTCTA TTCTTCGATT TGCATTTGGG AGTCAAAATA ACAAATTGAA AGATACAAAT 
AAGGAATTCG TGAAATTTCT TGAGAAGAAT ATGAAAAAGC TTCCGAAATC GACAGCAATT 
AATGAGACTT TTCGCTCAAA AGATGTCAGT GTTGGAGCAA ATTTCACAGT TGTACTCAAG 
AAAGAACCAG ATGCACCCTT GTTCTTGTAT ATGCAAAATA ACGGAAGCCT GCATGCTTCA 
GCATTGTTCT CTGATGCAAC AACTCAACAA TTGATAGCAC CAAATAGTAA TTTACTGAAA 
GATTCTCTAC GAAGCGTAGG AGTAAACGTC TGTGACACGA CAATGCCTCC ATTAACCAAA 
GGAATTCTTA TTGCTGAATA TGACTCAATT ATAGAAAGCA TCGCTGGCCT TCGATTGGGC 
GAAGAAATTG GTTTACAGCC GCGAATTCTG CTCAGGAAAA GTGATGTTGT AGAAGAATTA 
GGACTTCCTG AAGCTACTGA GAAGCTTCTT CCAATCGAGG TGGTTAACCG GGATTCTGAT 
GCTGGAACCA GTAAAAATTT TGACTTCAAT CTTTATTTCG AACAAATTCA TTCAAAAATC 
GGTCAAGTCG TACTGCTTGT TGATGTAGCA ACAACTACAA TGGATATTAT TGAAAGTGTG 
AATGCCGGAA TTCCATCACT CGAAAGTGTA GTTGTAATTG CAAATCGTCA AATCAGCGGA 
AGAGGACGAG GTGGAAATGA GTTCCTTTGT CCACGTGGGA TGGCAATGTT CAACTTTTCT 
TTTTCTATCT CAAAAAAGAG CAGAATCGCA AAACATCTTC CAATTCTCCA GCACATTTTC 
TGTGTGGCAC TTGTTGAAGC AGCACGGAAT CTATCTGGAT ATCCAGAGTT TCCACTGCAT 
ATCAAATGGC CAAATGATCT GTATTGTGAA CGATCTCATA AAGTTGGTGG AATGCTTCTT 
CAGTGCAGTA CTAGGGATGA TTCATTCCGA GTCAGTATCG GATGTGGAAT GAATGTTTCG 
AACGATAAAC CGACGATGTG CTTGAATGAT ATGCTGCCTA AAGAAGCCGA AACACGGATT 
ACAAAAGAAC AATTGATTGC GGAAACAATA AACAAATTCA CTTATTACAT GAAGGACTAT 
GAGGATAATG GCCCGGAAAC CTTCAAAAAG AAGTATCACG AGTACTGGCT GCATTCACAA 
CAAGAAGTTC TACTGTCAGA CTTCAATGAA CGAGTAACGA TTCGAGGAAT CGACGATGAC 
GGATACCTGC AAGTACGCAG CAAGTCCAAC CCGGATAAAA TATTCTCAAT CGGTGATGAT 
GGAAATACCT TCGACATGAT GAAGGGTCTA ATTCGTCATA AATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495257.3
CDS	151..3798
Translation	MILFVWSLVSSAIEAARKRHIANFLRALLAQQRVSRNYEMLIWKTIIKSSQYLSTQRASSSRLYRPTSEPKLDSTLVLQTDLIQVYAEKFETLRASEWRFCPSLWPCEEDSIYFVVHDEFMEKRQKEPGGDEKLEPNYFKLHEKCDRLAVHKTMTRCLYACSFDMFIDVVDAFSEKFLIDENFQVTKIFSMFAPPQELDRSSTSSLRPEMRTSTLSLASNSLLPRPLPILQHLFWGDRTGSTKFSTNDRYGSEGNLDMSPSRRHPGRPSSSATISNSARRRRYRSCNSPEHLESESSQLTLESGSEEFRSLPRYIRQLNQRDFGSPSNSITNGYIPDSVDFNSLPRYIRHLEKSVCDSTEDVTSFRSDRALPSSSSCSHPPTTSDSCTSSLAEEAASNSGIYKNQSSASRTNSNGSSTDRRTPDCHPINSGLKSHFTMDELRMIDETDSETVNTARELIEEDAENISFAKLAYQNTNGNAANKPEKENRNGSKFANGSVTIKLETSDNLNDKITNSDRTPTPYPPADSERPISEASSCHSESPPRDSKPPRSPSRVTIPTIDFSRLPPLAQQTARSKRSTSVVANNNRQNSQRRFSAFSTESDRGRSVSPSFEYYQNRSLRGFTSAANPHRVATRNSTVRQVPLYQFLTSRSSSFKAMCKPNSVLVYTGGNDSLYSDIRHRLSFLLPPDEITVFNVSIQALKKQPWAEKSTVCVILASTNDLDDEAWEKIQAYFNQNGKIIFVCQNKLLASITGCDSSKANASILRFAFGSQNNKLKDTNKEFVKFLEKNMKKLPKSTAINETFRSKDVSVGANFTVVLKKEPDAPLFLYMQNNGSLHASALFSDATTQQLIAPNSNLLKDSLRSVGVNVCDTTMPPLTKGILIAEYDSIIESIAGLRLGEEIGLQPRILLRKSDVVEELGLPEATEKLLPIEVVNRDSDAGTSKNFDFNLYFEQIHSKIGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNLSGYPEFPLHIKWPNDLYCERSHKVGGMLLQCSTRDDSFRVSIGCGMNVSNDKPTMCLNDMLPKEAETRITKEQLIAETINKFTYYMKDYEDNGPETFKKKYHEYWLHSQQEVLLSDFNERVTIRGIDDDGYLQVRSKSNPDKIFSIGDDGNTFDMMKGLIRHKY

Target ORF information:

RefSeq Version	NM_062856.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BPL/LPL catalytic domain-containing protein (bpl-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062856.7

ORF Insert Sequence:

ATGATACTTT TCGTTTGGTC ACTAGTTTCA TCGGCCATTG AGGCTGCTCG AAAACGTCAT 
ATTGCTAATT TCCTTCGTGC CTTACTTGCT CAACAACGGG TTTCTAGAAA CTATGAAATG 
CTCATATGGA AAACAATTAT TAAATCTTCA CAATATTTAT CAACTCAAAG AGCCAGTTCA 
TCGAGATTGT ACCGACCAAC TAGTGAACCA AAACTCGATT CGACACTTGT CTTGCAGACT 
GATTTAATAC AAGTTTATGC CGAAAAATTC GAAACGCTTC GTGCCAGCGA ATGGAGATTT 
TGTCCGTCTT TATGGCCTTG TGAAGAGGAT TCAATATACT TTGTAGTACA TGATGAATTC 
ATGGAAAAAC GACAAAAAGA GCCAGGAGGA GATGAAAAAT TGGAACCAAA CTATTTTAAA 
CTTCACGAAA AGTGTGATCG ATTGGCAGTT CACAAAACAA TGACCCGATG CTTATATGCT 
TGCTCATTCG ATATGTTCAT AGATGTTGTA GATGCATTTT CTGAAAAGTT TCTCATTGAC 
GAAAACTTTC AAGTTACCAA AATTTTCAGC ATGTTTGCAC CGCCACAAGA ATTAGATCGA 
TCGTCAACGT CATCTCTCCG ACCCGAAATG CGAACTTCAA CGTTATCACT TGCATCGAAT 
TCTCTCCTTC CGAGACCTCT TCCAATTTTA CAGCATTTGT TCTGGGGAGA TCGTACAGGA 
TCAACGAAAT TCTCGACTAA TGATCGATAT GGAAGTGAAG GAAATTTAGA CATGTCCCCG 
AGTCGACGAC ATCCAGGCCG TCCATCATCA TCTGCAACAA TATCCAATTC AGCCAGAAGA 
CGTCGTTATC GTTCTTGTAA TTCACCGGAA CATCTTGAAT CTGAATCCAG TCAATTGACT 
CTTGAAAGCG GAAGTGAAGA ATTCCGAAGT CTTCCTCGAT ATATCAGACA ACTAAATCAA 
CGGGATTTTG GAAGCCCTAG TAATAGTATT ACGAACGGAT ATATTCCTGA TTCCGTCGAC 
TTCAATAGTT TGCCCAGATA TATTCGTCAT CTGGAAAAGT CGGTTTGCGA CTCAACAGAA 
GACGTTACTT CTTTTCGATC GGATAGAGCA CTTCCATCGT CTTCTTCCTG TAGTCATCCT 
CCAACAACTT CTGATTCTTG TACAAGTAGT CTAGCAGAAG AAGCAGCATC GAACAGCGGA 
ATATATAAGA ATCAAAGTTC AGCATCAAGA ACTAATTCAA ACGGATCATC AACTGATCGA 
AGGACACCTG ATTGTCATCC AATAAATTCT GGATTAAAAA GTCATTTCAC AATGGATGAA 
CTACGTATGA TTGATGAAAC CGATTCGGAG ACTGTCAATA CAGCAAGGGA ATTAATTGAA 
GAGGATGCTG AAAACATTTC ATTTGCAAAA CTTGCTTATC AAAATACGAA CGGAAATGCG 
GCAAATAAAC CTGAGAAGGA AAATAGAAAT GGCTCCAAAT TTGCAAATGG AAGTGTTACC 
ATCAAACTGG AAACAAGTGA TAATCTAAAT GACAAGATCA CGAATTCCGA TAGAACACCA 
ACACCGTATC CCCCAGCTGA TTCTGAAAGA CCAATAAGTG AAGCTTCCTC GTGTCATTCT 
GAATCTCCTC CGCGAGACTC GAAGCCTCCA CGAAGTCCAT CTAGAGTTAC GATTCCAACA 
ATCGACTTTT CTCGACTTCC TCCACTTGCT CAACAAACTG CCCGTTCTAA ACGAAGTACC 
TCTGTCGTCG CCAACAATAA CCGGCAAAAT TCCCAGCGTC GTTTCTCAGC TTTCTCAACG 
GAAAGCGATC GAGGAAGATC TGTCAGTCCT TCATTTGAAT ATTATCAAAA TCGATCTCTA 
CGCGGATTCA CTTCTGCAGC CAATCCACAT CGCGTCGCTA CTCGAAATTC CACAGTTCGT 
CAAGTACCAC TTTACCAGTT CCTGACATCA AGAAGTTCAT CATTCAAGGC AATGTGTAAA 
CCGAACTCTG TTCTCGTATA TACCGGAGGG AATGATAGTC TTTATTCTGA TATCCGTCAT 
CGATTATCAT TTCTTTTACC TCCAGATGAG ATCACTGTTT TTAATGTGTC AATTCAAGCT 
TTGAAAAAAC AGCCATGGGC AGAAAAATCA ACGGTTTGTG TAATACTTGC GTCTACGAAC 
GATTTGGACG ATGAAGCATG GGAGAAAATT CAAGCATATT TCAATCAAAA CGGAAAAATA 
ATTTTTGTCT GCCAGAATAA GCTATTGGCA AGTATCACAG GTTGTGATTC GAGTAAAGCG 
AACGCTTCTA TTCTTCGATT TGCATTTGGG AGTCAAAATA ACAAATTGAA AGATACAAAT 
AAGGAATTCG TGAAATTTCT TGAGAAGAAT ATGAAAAAGC TTCCGAAATC GACAGCAATT 
AATGAGACTT TTCGCTCAAA AGATGTCAGT GTTGGAGCAA ATTTCACAGT TGTACTCAAG 
AAAGAACCAG ATGCACCCTT GTTCTTGTAT ATGCAAAATA ACGGAAGCCT GCATGCTTCA 
GCATTGTTCT CTGATGCAAC AACTCAACAA TTGATAGCAC CAAATAGTAA TTTACTGAAA 
GATTCTCTAC GAAGCGTAGG AGTAAACGTC TGTGACACGA CAATGCCTCC ATTAACCAAA 
GGAATTCTTA TTGCTGAATA TGACTCAATT ATAGAAAGCA TCGCTGGCCT TCGATTGGGC 
GAAGAAATTG GTTTACAGCC GCGAATTCTG CTCAGGAAAA GTGATGTTGT AGAAGAATTA 
GGACTTCCTG AAGCTACTGA GAAGCTTCTT CCAATCGAGG TGGTTAACCG GGATTCTGAT 
GCTGGAACCA GTAAAAATTT TGACTTCAAT CTTTATTTCG AACAAATTCA TTCAAAAATC 
GGTCAAGTCG TACTGCTTGT TGATGTAGCA ACAACTACAA TGGATATTAT TGAAAGTGTG 
AATGCCGGAA TTCCATCACT CGAAAGTGTA GTTGTAATTG CAAATCGTCA AATCAGCGGA 
AGAGGACGAG GTGGAAATGA GTTCCTTTGT CCACGTGGGA TGGCAATGTT CAACTTTTCT 
TTTTCTATCT CAAAAAAGAG CAGAATCGCA AAACATCTTC CAATTCTCCA GCACATTTTC 
TGTGTGGCAC TTGTTGAAGC AGCACGGAAT CTATCTGGAT ATCCAGAGTT TCCACTGCAT 
ATCAAATGGC CAAATGATCT GTATTGTGAA CGATCTCATA AAGTTGGTGG AATGCTTCTT 
CAGTGCAGTA CTAGGGATGA TTCATTCCGA GTCAGTATCG GATGTGGAAT GAATGTTTCG 
AACGATAAAC CGACGATGTG CTTGAATGAT ATGCTGCCTA AAGAAGCCGA AACACGGATT 
ACAAAAGAAC AATTGATTGC GGAAACAATA AACAAATTCA CTTATTACAT GAAGGACTAT 
GAGGATAATG GCCCGGAAAC CTTCAAAAAG AAGTATCACG AGTACTGGCT GCATTCACAA 
CAAGAAGTTC TACTGTCAGA CTTCAATGAA CGAGTAACGA TTCGAGGAAT CGACGATGAC 
GGATACCTGC AAGTACGCAG CAAGTCCAAC CCGGATAAAA TATTCTCAAT CGGTGATGAT 
GGAAATACCT TCGACATGAT GAAGGGTCTA ATTCGTCATA AATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bpl-1 ( NM_062856.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495257.3 Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.

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CloneID	OCa338817
Clone ID Related Accession (Same CDS sequence)	NM_062856.7 , NM_062856.6
Accession Version	NM_062856.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Biotin Protein Ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_062856.5. COMPLETENESS: incomplete on both ends.

ATGATACTTT TCGTTTGGTC ACTAGTTTCA TCGGCCATTG AGGCTGCTCG AAAACGTCAT 
ATTGCTAATT TCCTTCGTGC CTTACTTGCT CAACAACGGG TTTCTAGAAA CTATGAAATG 
CTCATATGGA AAACAATTAT TAAATCTTCA CAATATTTAT CAACTCAAAG AGCCAGTTCA 
TCGAGATTGT ACCGACCAAC TAGTGAACCA AAACTCGATT CGACACTTGT CTTGCAGACT 
GATTTAATAC AAGTTTATGC CGAAAAATTC GAAACGCTTC GTGCCAGCGA ATGGAGATTT 
TGTCCGTCTT TATGGCCTTG TGAAGAGGAT TCAATATACT TTGTAGTACA TGATGAATTC 
ATGGAAAAAC GACAAAAAGA GCCAGGAGGA GATGAAAAAT TGGAACCAAA CTATTTTAAA 
CTTCACGAAA AGTGTGATCG ATTGGCAGTT CACAAAACAA TGACCCGATG CTTATATGCT 
TGCTCATTCG ATATGTTCAT AGATGTTGTA GATGCATTTT CTGAAAAGTT TCTCATTGAC 
GAAAACTTTC AAGTTACCAA AATTTTCAGC ATGTTTGCAC CGCCACAAGA ATTAGATCGA 
TCGTCAACGT CATCTCTCCG ACCCGAAATG CGAACTTCAA CGTTATCACT TGCATCGAAT 
TCTCTCCTTC CGAGACCTCT TCCAATTTTA CAGCATTTGT TCTGGGGAGA TCGTACAGGA 
TCAACGAAAT TCTCGACTAA TGATCGATAT GGAAGTGAAG GAAATTTAGA CATGTCCCCG 
AGTCGACGAC ATCCAGGCCG TCCATCATCA TCTGCAACAA TATCCAATTC AGCCAGAAGA 
CGTCGTTATC GTTCTTGTAA TTCACCGGAA CATCTTGAAT CTGAATCCAG TCAATTGACT 
CTTGAAAGCG GAAGTGAAGA ATTCCGAAGT CTTCCTCGAT ATATCAGACA ACTAAATCAA 
CGGGATTTTG GAAGCCCTAG TAATAGTATT ACGAACGGAT ATATTCCTGA TTCCGTCGAC 
TTCAATAGTT TGCCCAGATA TATTCGTCAT CTGGAAAAGT CGGTTTGCGA CTCAACAGAA 
GACGTTACTT CTTTTCGATC GGATAGAGCA CTTCCATCGT CTTCTTCCTG TAGTCATCCT 
CCAACAACTT CTGATTCTTG TACAAGTAGT CTAGCAGAAG AAGCAGCATC GAACAGCGGA 
ATATATAAGA ATCAAAGTTC AGCATCAAGA ACTAATTCAA ACGGATCATC AACTGATCGA 
AGGACACCTG ATTGTCATCC AATAAATTCT GGATTAAAAA GTCATTTCAC AATGGATGAA 
CTACGTATGA TTGATGAAAC CGATTCGGAG ACTGTCAATA CAGCAAGGGA ATTAATTGAA 
GAGGATGCTG AAAACATTTC ATTTGCAAAA CTTGCTTATC AAAATACGAA CGGAAATGCG 
GCAAATAAAC CTGAGAAGGA AAATAGAAAT GGCTCCAAAT TTGCAAATGG AAGTGTTACC 
ATCAAACTGG AAACAAGTGA TAATCTAAAT GACAAGATCA CGAATTCCGA TAGAACACCA 
ACACCGTATC CCCCAGCTGA TTCTGAAAGA CCAATAAGTG AAGCTTCCTC GTGTCATTCT 
GAATCTCCTC CGCGAGACTC GAAGCCTCCA CGAAGTCCAT CTAGAGTTAC GATTCCAACA 
ATCGACTTTT CTCGACTTCC TCCACTTGCT CAACAAACTG CCCGTTCTAA ACGAAGTACC 
TCTGTCGTCG CCAACAATAA CCGGCAAAAT TCCCAGCGTC GTTTCTCAGC TTTCTCAACG 
GAAAGCGATC GAGGAAGATC TGTCAGTCCT TCATTTGAAT ATTATCAAAA TCGATCTCTA 
CGCGGATTCA CTTCTGCAGC CAATCCACAT CGCGTCGCTA CTCGAAATTC CACAGTTCGT 
CAAGTACCAC TTTACCAGTT CCTGACATCA AGAAGTTCAT CATTCAAGGC AATGTGTAAA 
CCGAACTCTG TTCTCGTATA TACCGGAGGG AATGATAGTC TTTATTCTGA TATCCGTCAT 
CGATTATCAT TTCTTTTACC TCCAGATGAG ATCACTGTTT TTAATGTGTC AATTCAAGCT 
TTGAAAAAAC AGCCATGGGC AGAAAAATCA ACGGTTTGTG TAATACTTGC GTCTACGAAC 
GATTTGGACG ATGAAGCATG GGAGAAAATT CAAGCATATT TCAATCAAAA CGGAAAAATA 
ATTTTTGTCT GCCAGAATAA GCTATTGGCA AGTATCACAG GTTGTGATTC GAGTAAAGCG 
AACGCTTCTA TTCTTCGATT TGCATTTGGG AGTCAAAATA ACAAATTGAA AGATACAAAT 
AAGGAATTCG TGAAATTTCT TGAGAAGAAT ATGAAAAAGC TTCCGAAATC GACAGCAATT 
AATGAGACTT TTCGCTCAAA AGATGTCAGT GTTGGAGCAA ATTTCACAGT TGTACTCAAG 
AAAGAACCAG ATGCACCCTT GTTCTTGTAT ATGCAAAATA ACGGAAGCCT GCATGCTTCA 
GCATTGTTCT CTGATGCAAC AACTCAACAA TTGATAGCAC CAAATAGTAA TTTACTGAAA 
GATTCTCTAC GAAGCGTAGG AGTAAACGTC TGTGACACGA CAATGCCTCC ATTAACCAAA 
GGAATTCTTA TTGCTGAATA TGACTCAATT ATAGAAAGCA TCGCTGGCCT TCGATTGGGC 
GAAGAAATTG GTTTACAGCC GCGAATTCTG CTCAGGAAAA GTGATGTTGT AGAAGAATTA 
GGACTTCCTG AAGCTACTGA GAAGCTTCTT CCAATCGAGG TGGTTAACCG GGATTCTGAT 
GCTGGAACCA GTAAAAATTT TGACTTCAAT CTTTATTTCG AACAAATTCA TTCAAAAATC 
GGTCAAGTCG TACTGCTTGT TGATGTAGCA ACAACTACAA TGGATATTAT TGAAAGTGTG 
AATGCCGGAA TTCCATCACT CGAAAGTGTA GTTGTAATTG CAAATCGTCA AATCAGCGGA 
AGAGGACGAG GTGGAAATGA GTTCCTTTGT CCACGTGGGA TGGCAATGTT CAACTTTTCT 
TTTTCTATCT CAAAAAAGAG CAGAATCGCA AAACATCTTC CAATTCTCCA GCACATTTTC 
TGTGTGGCAC TTGTTGAAGC AGCACGGAAT CTATCTGGAT ATCCAGAGTT TCCACTGCAT 
ATCAAATGGC CAAATGATCT GTATTGTGAA CGATCTCATA AAGTTGGTGG AATGCTTCTT 
CAGTGCAGTA CTAGGGATGA TTCATTCCGA GTCAGTATCG GATGTGGAAT GAATGTTTCG 
AACGATAAAC CGACGATGTG CTTGAATGAT ATGCTGCCTA AAGAAGCCGA AACACGGATT 
ACAAAAGAAC AATTGATTGC GGAAACAATA AACAAATTCA CTTATTACAT GAAGGACTAT 
GAGGATAATG GCCCGGAAAC CTTCAAAAAG AAGTATCACG AGTACTGGCT GCATTCACAA 
CAAGAAGTTC TACTGTCAGA CTTCAATGAA CGAGTAACGA TTCGAGGAAT CGACGATGAC 
GGATACCTGC AAGTACGCAG CAAGTCCAAC CCGGATAAAA TATTCTCAAT CGGTGATGAT 
GGAAATACCT TCGACATGAT GAAGGGTCTA ATTCGTCATA AATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495257.3
CDS	1..3648
Translation	MILFVWSLVSSAIEAARKRHIANFLRALLAQQRVSRNYEMLIWKTIIKSSQYLSTQRASSSRLYRPTSEPKLDSTLVLQTDLIQVYAEKFETLRASEWRFCPSLWPCEEDSIYFVVHDEFMEKRQKEPGGDEKLEPNYFKLHEKCDRLAVHKTMTRCLYACSFDMFIDVVDAFSEKFLIDENFQVTKIFSMFAPPQELDRSSTSSLRPEMRTSTLSLASNSLLPRPLPILQHLFWGDRTGSTKFSTNDRYGSEGNLDMSPSRRHPGRPSSSATISNSARRRRYRSCNSPEHLESESSQLTLESGSEEFRSLPRYIRQLNQRDFGSPSNSITNGYIPDSVDFNSLPRYIRHLEKSVCDSTEDVTSFRSDRALPSSSSCSHPPTTSDSCTSSLAEEAASNSGIYKNQSSASRTNSNGSSTDRRTPDCHPINSGLKSHFTMDELRMIDETDSETVNTARELIEEDAENISFAKLAYQNTNGNAANKPEKENRNGSKFANGSVTIKLETSDNLNDKITNSDRTPTPYPPADSERPISEASSCHSESPPRDSKPPRSPSRVTIPTIDFSRLPPLAQQTARSKRSTSVVANNNRQNSQRRFSAFSTESDRGRSVSPSFEYYQNRSLRGFTSAANPHRVATRNSTVRQVPLYQFLTSRSSSFKAMCKPNSVLVYTGGNDSLYSDIRHRLSFLLPPDEITVFNVSIQALKKQPWAEKSTVCVILASTNDLDDEAWEKIQAYFNQNGKIIFVCQNKLLASITGCDSSKANASILRFAFGSQNNKLKDTNKEFVKFLEKNMKKLPKSTAINETFRSKDVSVGANFTVVLKKEPDAPLFLYMQNNGSLHASALFSDATTQQLIAPNSNLLKDSLRSVGVNVCDTTMPPLTKGILIAEYDSIIESIAGLRLGEEIGLQPRILLRKSDVVEELGLPEATEKLLPIEVVNRDSDAGTSKNFDFNLYFEQIHSKIGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNLSGYPEFPLHIKWPNDLYCERSHKVGGMLLQCSTRDDSFRVSIGCGMNVSNDKPTMCLNDMLPKEAETRITKEQLIAETINKFTYYMKDYEDNGPETFKKKYHEYWLHSQQEVLLSDFNERVTIRGIDDDGYLQVRSKSNPDKIFSIGDDGNTFDMMKGLIRHKY

Target ORF information:

RefSeq Version	NM_062856.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Biotin Protein Ligase (bpl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062856.6

ORF Insert Sequence:

ATGATACTTT TCGTTTGGTC ACTAGTTTCA TCGGCCATTG AGGCTGCTCG AAAACGTCAT 
ATTGCTAATT TCCTTCGTGC CTTACTTGCT CAACAACGGG TTTCTAGAAA CTATGAAATG 
CTCATATGGA AAACAATTAT TAAATCTTCA CAATATTTAT CAACTCAAAG AGCCAGTTCA 
TCGAGATTGT ACCGACCAAC TAGTGAACCA AAACTCGATT CGACACTTGT CTTGCAGACT 
GATTTAATAC AAGTTTATGC CGAAAAATTC GAAACGCTTC GTGCCAGCGA ATGGAGATTT 
TGTCCGTCTT TATGGCCTTG TGAAGAGGAT TCAATATACT TTGTAGTACA TGATGAATTC 
ATGGAAAAAC GACAAAAAGA GCCAGGAGGA GATGAAAAAT TGGAACCAAA CTATTTTAAA 
CTTCACGAAA AGTGTGATCG ATTGGCAGTT CACAAAACAA TGACCCGATG CTTATATGCT 
TGCTCATTCG ATATGTTCAT AGATGTTGTA GATGCATTTT CTGAAAAGTT TCTCATTGAC 
GAAAACTTTC AAGTTACCAA AATTTTCAGC ATGTTTGCAC CGCCACAAGA ATTAGATCGA 
TCGTCAACGT CATCTCTCCG ACCCGAAATG CGAACTTCAA CGTTATCACT TGCATCGAAT 
TCTCTCCTTC CGAGACCTCT TCCAATTTTA CAGCATTTGT TCTGGGGAGA TCGTACAGGA 
TCAACGAAAT TCTCGACTAA TGATCGATAT GGAAGTGAAG GAAATTTAGA CATGTCCCCG 
AGTCGACGAC ATCCAGGCCG TCCATCATCA TCTGCAACAA TATCCAATTC AGCCAGAAGA 
CGTCGTTATC GTTCTTGTAA TTCACCGGAA CATCTTGAAT CTGAATCCAG TCAATTGACT 
CTTGAAAGCG GAAGTGAAGA ATTCCGAAGT CTTCCTCGAT ATATCAGACA ACTAAATCAA 
CGGGATTTTG GAAGCCCTAG TAATAGTATT ACGAACGGAT ATATTCCTGA TTCCGTCGAC 
TTCAATAGTT TGCCCAGATA TATTCGTCAT CTGGAAAAGT CGGTTTGCGA CTCAACAGAA 
GACGTTACTT CTTTTCGATC GGATAGAGCA CTTCCATCGT CTTCTTCCTG TAGTCATCCT 
CCAACAACTT CTGATTCTTG TACAAGTAGT CTAGCAGAAG AAGCAGCATC GAACAGCGGA 
ATATATAAGA ATCAAAGTTC AGCATCAAGA ACTAATTCAA ACGGATCATC AACTGATCGA 
AGGACACCTG ATTGTCATCC AATAAATTCT GGATTAAAAA GTCATTTCAC AATGGATGAA 
CTACGTATGA TTGATGAAAC CGATTCGGAG ACTGTCAATA CAGCAAGGGA ATTAATTGAA 
GAGGATGCTG AAAACATTTC ATTTGCAAAA CTTGCTTATC AAAATACGAA CGGAAATGCG 
GCAAATAAAC CTGAGAAGGA AAATAGAAAT GGCTCCAAAT TTGCAAATGG AAGTGTTACC 
ATCAAACTGG AAACAAGTGA TAATCTAAAT GACAAGATCA CGAATTCCGA TAGAACACCA 
ACACCGTATC CCCCAGCTGA TTCTGAAAGA CCAATAAGTG AAGCTTCCTC GTGTCATTCT 
GAATCTCCTC CGCGAGACTC GAAGCCTCCA CGAAGTCCAT CTAGAGTTAC GATTCCAACA 
ATCGACTTTT CTCGACTTCC TCCACTTGCT CAACAAACTG CCCGTTCTAA ACGAAGTACC 
TCTGTCGTCG CCAACAATAA CCGGCAAAAT TCCCAGCGTC GTTTCTCAGC TTTCTCAACG 
GAAAGCGATC GAGGAAGATC TGTCAGTCCT TCATTTGAAT ATTATCAAAA TCGATCTCTA 
CGCGGATTCA CTTCTGCAGC CAATCCACAT CGCGTCGCTA CTCGAAATTC CACAGTTCGT 
CAAGTACCAC TTTACCAGTT CCTGACATCA AGAAGTTCAT CATTCAAGGC AATGTGTAAA 
CCGAACTCTG TTCTCGTATA TACCGGAGGG AATGATAGTC TTTATTCTGA TATCCGTCAT 
CGATTATCAT TTCTTTTACC TCCAGATGAG ATCACTGTTT TTAATGTGTC AATTCAAGCT 
TTGAAAAAAC AGCCATGGGC AGAAAAATCA ACGGTTTGTG TAATACTTGC GTCTACGAAC 
GATTTGGACG ATGAAGCATG GGAGAAAATT CAAGCATATT TCAATCAAAA CGGAAAAATA 
ATTTTTGTCT GCCAGAATAA GCTATTGGCA AGTATCACAG GTTGTGATTC GAGTAAAGCG 
AACGCTTCTA TTCTTCGATT TGCATTTGGG AGTCAAAATA ACAAATTGAA AGATACAAAT 
AAGGAATTCG TGAAATTTCT TGAGAAGAAT ATGAAAAAGC TTCCGAAATC GACAGCAATT 
AATGAGACTT TTCGCTCAAA AGATGTCAGT GTTGGAGCAA ATTTCACAGT TGTACTCAAG 
AAAGAACCAG ATGCACCCTT GTTCTTGTAT ATGCAAAATA ACGGAAGCCT GCATGCTTCA 
GCATTGTTCT CTGATGCAAC AACTCAACAA TTGATAGCAC CAAATAGTAA TTTACTGAAA 
GATTCTCTAC GAAGCGTAGG AGTAAACGTC TGTGACACGA CAATGCCTCC ATTAACCAAA 
GGAATTCTTA TTGCTGAATA TGACTCAATT ATAGAAAGCA TCGCTGGCCT TCGATTGGGC 
GAAGAAATTG GTTTACAGCC GCGAATTCTG CTCAGGAAAA GTGATGTTGT AGAAGAATTA 
GGACTTCCTG AAGCTACTGA GAAGCTTCTT CCAATCGAGG TGGTTAACCG GGATTCTGAT 
GCTGGAACCA GTAAAAATTT TGACTTCAAT CTTTATTTCG AACAAATTCA TTCAAAAATC 
GGTCAAGTCG TACTGCTTGT TGATGTAGCA ACAACTACAA TGGATATTAT TGAAAGTGTG 
AATGCCGGAA TTCCATCACT CGAAAGTGTA GTTGTAATTG CAAATCGTCA AATCAGCGGA 
AGAGGACGAG GTGGAAATGA GTTCCTTTGT CCACGTGGGA TGGCAATGTT CAACTTTTCT 
TTTTCTATCT CAAAAAAGAG CAGAATCGCA AAACATCTTC CAATTCTCCA GCACATTTTC 
TGTGTGGCAC TTGTTGAAGC AGCACGGAAT CTATCTGGAT ATCCAGAGTT TCCACTGCAT 
ATCAAATGGC CAAATGATCT GTATTGTGAA CGATCTCATA AAGTTGGTGG AATGCTTCTT 
CAGTGCAGTA CTAGGGATGA TTCATTCCGA GTCAGTATCG GATGTGGAAT GAATGTTTCG 
AACGATAAAC CGACGATGTG CTTGAATGAT ATGCTGCCTA AAGAAGCCGA AACACGGATT 
ACAAAAGAAC AATTGATTGC GGAAACAATA AACAAATTCA CTTATTACAT GAAGGACTAT 
GAGGATAATG GCCCGGAAAC CTTCAAAAAG AAGTATCACG AGTACTGGCT GCATTCACAA 
CAAGAAGTTC TACTGTCAGA CTTCAATGAA CGAGTAACGA TTCGAGGAAT CGACGATGAC 
GGATACCTGC AAGTACGCAG CAAGTCCAAC CCGGATAAAA TATTCTCAAT CGGTGATGAT 
GGAAATACCT TCGACATGAT GAAGGGTCTA ATTCGTCATA AATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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bpl-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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