ORF » Species Summary » Schizosaccharomyces pombe » tif225 cDNA ORF clone

tif225 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following tif225 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tif225 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102657 NM_001019708.2
Latest version!		 Schizosaccharomyces pombe putative translation initiation factor eIF2B epsilon subunit tif225 (tif225), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    tif225 ( NM_001019708.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594285.1 Schizosaccharomyces pombe putative translation initiation factor eIF2B epsilon subunit tif225 (tif225), mRNA.
    Price & Availability↑
    CloneID OSc102657
    Clone ID Related Accession (Same CDS sequence) NM_001019708.2
    Accession Version NM_001019708.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative translation initiation factor eIF2B epsilon subunit tif225
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019708.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCTCCAT CCAAAGGGCT GAATGGCAAG CTAGAAAAGC CAAAACACGC TCTGCAAGCG 
    ATTGTTTTGT CGGATTCTTA TAATTATCGG TTTCGCCCTC TTACATTAGA TAAACCTCGC 
    TGTCTTCTTC CTTTAGCAAA TACCCCTTTA ATAGAGTACA CTTTTGAGTT TTTGGCATTG 
    GCCGGGGTGC AAGAAGTTTA TGTATTTTGC TGTGCCCATG CAGGACAAAT TCGAGAATAT 
    ATAGAAAAGT CAAAGTGGAA TCTTCCCTCT TCCCCCTTTA GTGTAAACAC GATTGTGTCA 
    AGAGAATCTC TGTCGGTTGG TGATGCTTTA AGAGAGCTCG ATTCAAAACA GCTAATTACC 
    TCCGATTTCA TACTCGTATC AGGCGATGTG GTATCCAATG TGCCTCTAAA CGAAGTTTTG 
    AAAGAACATC GGAAGCGCCG TGAAGATGAT AAAAATGCTA TTATGACAAT GGTTGTACGA 
    GAAGCAAGTC CATTTCATCG TACACGCGCC CGTACAGAGA GTTCTGTTTT CGTGATAGAT 
    AAAAAGACCA GCCAATGCGT GCATTATCAA GCTAACGAAA GGGGAAAACA TTATGTTTCT 
    ATGGATCCCG AAATCTTTAA TGAGCATGAA GAGTTGGAGG TTCGAAATGA TTTGATTGAC 
    TGTCAAATTG ACATTTGCTC CAATGATGTA CCTGCCCTTT TTACTGAAAA CTTTGATTAC 
    CAAGACATTC GAAAAGACTT CGTATATGGT GTTTTAACCT CTGATCTTTT AGGAAAAAAG 
    ATTCATTGCC ATGTTGCTAA AGAAAATTAT GCAGCTCGTG TTCGTAGTCT TCAAACTTAT 
    GATGCTATTA GCAAAGATGT TCTTTCTCGA TGGGTCTATC CTTTTGTTCC CGATAGCAAT 
    CTTCTTAACC AAACTTTTAG TTATCAAAGA CATCAGATTT ATAAGGAAGA AGACGTTGTT 
    CTTGCTCGTT CTTGCATTAT TAAGGCACGA ACTTTGATCG GTGCCTATAC CAAAGTCGGT 
    GATGCGTCTG TGGTTGCGAA TACTATCATT GGTAGGAATT GTACCATAGG CAGCAATTGT 
    AGTATAGACA GTGCTTTCCT TTGGGAAGAT GTTGTCATTG GAGATAATTG TCGAATTGGC 
    AAAGCCATTC TTGCCAATTC TGTAAAAATT GGAAATAATT GTTCAATTGA AGATGGTGCT 
    ATAGTAGCTG CAGGCGTTGT TATTGGGGAT AATACAATAA TTGAAAAAAA TAAGCGTCTT 
    ACAACTTTCG AGAGTCACTC TCAAGGAACT TTGAACGATC CATCTTTGGT AGGGATTGGT 
    GGACGTGGTC AAGAATATCA TGCCGAAGAG GATTCTGATG ATGAGGGAGA GTTTATGGAG 
    GCTTCAGGGC TTATTGAAAG TACAAATGAG TTACATTTAT CTGATTCAGA ATCTTCAGAG 
    ACAAGTTCGT CTTCTGAAGA AGATATGGAA TTTATTCCCT TTTCAGCTAG ACGCGATTCT 
    GCCAATACAA TTAACTCAGA GGATTTCGAT GAAGGAGATT TCAACAAGGA AGCTCAGCAG 
    AGTCTTGAAC GTGCCTTTGA AGAAAACCAC CAAATTGATA TTGCTGCTCT TGAACTCAAT 
    ACCCTCCGAA TGGCCATGAA TGCCAACTAT CACGAGGTGC GATCCGCAAT TGTTCTTGCT 
    CTTTTAAGGC GTATCATGCA CTTAGATGTA TCCCCCAAGG AGGCACTAGC AAAAGTAATG 
    ACACGTTGGG GTCCATTGCT TGCAAAACTG ACATTTAGCC ATGAAGAGCA AGTTGACAAC 
    GTATTAACGC TGCAGAAGTA CTGTGTCCGT TTGAGTATGA CTCGGCACTT TTTACAACTT 
    TTGGGATATT TCTACCAATT AGAAATTGCT GAAGAGAATG CCATTCAAGA ATGGTATTCT 
    GATCCCCGCT CTAGCGAAGG AGAATTGGCT GCGCTTCGTG ATGCCGGTGG CAAACAATTT 
    GTCGACTGGT TGAATACTGC TGAAAGTGAA AGCGAGAGTG AAGAAGGGTC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594285.1
    CDS16..2052
    Translation

    Target ORF information:

    RefSeq Version NM_001019708.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative translation initiation factor eIF2B epsilon subunit tif225 (tif225), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001019708.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCTCCAT CCAAAGGGCT GAATGGCAAG CTAGAAAAGC CAAAACACGC TCTGCAAGCG 
    ATTGTTTTGT CGGATTCTTA TAATTATCGG TTTCGCCCTC TTACATTAGA TAAACCTCGC 
    TGTCTTCTTC CTTTAGCAAA TACCCCTTTA ATAGAGTACA CTTTTGAGTT TTTGGCATTG 
    GCCGGGGTGC AAGAAGTTTA TGTATTTTGC TGTGCCCATG CAGGACAAAT TCGAGAATAT 
    ATAGAAAAGT CAAAGTGGAA TCTTCCCTCT TCCCCCTTTA GTGTAAACAC GATTGTGTCA 
    AGAGAATCTC TGTCGGTTGG TGATGCTTTA AGAGAGCTCG ATTCAAAACA GCTAATTACC 
    TCCGATTTCA TACTCGTATC AGGCGATGTG GTATCCAATG TGCCTCTAAA CGAAGTTTTG 
    AAAGAACATC GGAAGCGCCG TGAAGATGAT AAAAATGCTA TTATGACAAT GGTTGTACGA 
    GAAGCAAGTC CATTTCATCG TACACGCGCC CGTACAGAGA GTTCTGTTTT CGTGATAGAT 
    AAAAAGACCA GCCAATGCGT GCATTATCAA GCTAACGAAA GGGGAAAACA TTATGTTTCT 
    ATGGATCCCG AAATCTTTAA TGAGCATGAA GAGTTGGAGG TTCGAAATGA TTTGATTGAC 
    TGTCAAATTG ACATTTGCTC CAATGATGTA CCTGCCCTTT TTACTGAAAA CTTTGATTAC 
    CAAGACATTC GAAAAGACTT CGTATATGGT GTTTTAACCT CTGATCTTTT AGGAAAAAAG 
    ATTCATTGCC ATGTTGCTAA AGAAAATTAT GCAGCTCGTG TTCGTAGTCT TCAAACTTAT 
    GATGCTATTA GCAAAGATGT TCTTTCTCGA TGGGTCTATC CTTTTGTTCC CGATAGCAAT 
    CTTCTTAACC AAACTTTTAG TTATCAAAGA CATCAGATTT ATAAGGAAGA AGACGTTGTT 
    CTTGCTCGTT CTTGCATTAT TAAGGCACGA ACTTTGATCG GTGCCTATAC CAAAGTCGGT 
    GATGCGTCTG TGGTTGCGAA TACTATCATT GGTAGGAATT GTACCATAGG CAGCAATTGT 
    AGTATAGACA GTGCTTTCCT TTGGGAAGAT GTTGTCATTG GAGATAATTG TCGAATTGGC 
    AAAGCCATTC TTGCCAATTC TGTAAAAATT GGAAATAATT GTTCAATTGA AGATGGTGCT 
    ATAGTAGCTG CAGGCGTTGT TATTGGGGAT AATACAATAA TTGAAAAAAA TAAGCGTCTT 
    ACAACTTTCG AGAGTCACTC TCAAGGAACT TTGAACGATC CATCTTTGGT AGGGATTGGT 
    GGACGTGGTC AAGAATATCA TGCCGAAGAG GATTCTGATG ATGAGGGAGA GTTTATGGAG 
    GCTTCAGGGC TTATTGAAAG TACAAATGAG TTACATTTAT CTGATTCAGA ATCTTCAGAG 
    ACAAGTTCGT CTTCTGAAGA AGATATGGAA TTTATTCCCT TTTCAGCTAG ACGCGATTCT 
    GCCAATACAA TTAACTCAGA GGATTTCGAT GAAGGAGATT TCAACAAGGA AGCTCAGCAG 
    AGTCTTGAAC GTGCCTTTGA AGAAAACCAC CAAATTGATA TTGCTGCTCT TGAACTCAAT 
    ACCCTCCGAA TGGCCATGAA TGCCAACTAT CACGAGGTGC GATCCGCAAT TGTTCTTGCT 
    CTTTTAAGGC GTATCATGCA CTTAGATGTA TCCCCCAAGG AGGCACTAGC AAAAGTAATG 
    ACACGTTGGG GTCCATTGCT TGCAAAACTG ACATTTAGCC ATGAAGAGCA AGTTGACAAC 
    GTATTAACGC TGCAGAAGTA CTGTGTCCGT TTGAGTATGA CTCGGCACTT TTTACAACTT 
    TTGGGATATT TCTACCAATT AGAAATTGCT GAAGAGAATG CCATTCAAGA ATGGTATTCT 
    GATCCCCGCT CTAGCGAAGG AGAATTGGCT GCGCTTCGTG ATGCCGGTGG CAAACAATTT 
    GTCGACTGGT TGAATACTGC TGAAAGTGAA AGCGAGAGTG AAGAAGGGTC AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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