Eif2b5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif2b5
Entrez Gene ID	224045
Full Name	eukaryotic translation initiation factor 2B, subunit 5 epsilon
Synonyms	C81315
General protein information	Preferred Names eukaryotic translation initiation factor 2B, subunit 5 epsilon Names translation initiation factor eIF-2B subunit epsilon eIF-2B GDP-GTP exchange factor subunit epsilon
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_172265.2	NP_758469.1	translation initiation factor eIF-2B subunit epsilon

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2B5	XP_001139517.1
Gallus gallus (chicken)	EIF2B5	XP_422755.3
Drosophila melanogaster (fruit fly)	eIF2B-epsilon	NP_996329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000082	XP_001687758.1
Arabidopsis thaliana (thale cress)	AT2G34970	NP_181042.1
	EIF2B5	XP_001104122.1
Danio rerio (zebrafish)	eif2b5	NP_001107069.1
Schizosaccharomyces pombe (fission yeast)	tif225	NP_594285.1
Arabidopsis thaliana (thale cress)	AT4G18300	NP_193564.1
Xenopus tropicalis (tropical clawed frog)	eif2b5	XP_002935185.2
Mus musculus (house mouse)	Eif2b5	NP_758469.1
Saccharomyces cerevisiae (baker's yeast)	GCD6	NP_010497.3
Eremothecium gossypii	AGOS_AGL094W	NP_986572.1
Canis lupus familiaris (dog)	EIF2B5	XP_003640192.1
Bos taurus (cattle)	EIF2B5	XP_002684924.1
Rattus norvegicus (Norway rat)	Eif2b5	NP_620221.2
Kluyveromyces lactis	KLLA0A04235g	XP_451187.1
Neurospora crassa	NCU02414	XP_959596.1
Homo sapiens (human)	EIF2B5	NP_003898.2
Magnaporthe oryzae (rice blast fungus)	MGG_03517	XP_003716402.1
Arabidopsis thaliana (thale cress)	AT3G02270	NP_186876.1
Oryza sativa (rice)	Os02g0812400	NP_001048478.1

Gene Ontology
Bibliography

Related articles in PubMed

Affected astrocytes in the spinal cord of the leukodystrophy vanishing white matter.
Leferink PS, Breeuwsma N, Bugiani M, van der Knaap MS, Heine VM
Glia(2017 Dec)

Mutant eIF2B leads to impaired mitochondrial oxidative phosphorylation in vanishing white matter disease.
Raini G, Sharet R, Herrero M, Atzmon A, Shenoy A, Geiger T, Elroy-Stein O
Journal of neurochemistry141(5)694-707(2017 Jun)

Astrocytes are central in the pathomechanisms of vanishing white matter.
Dooves S, Bugiani M, Postma NL, Polder E, Land N, Horan ST, van Deijk AL, van de Kreeke A, Jacobs G, Vuong C, Klooster J, Kamermans M, Wortel J, Loos M, Wisse LE, Scheper GC, Abbink TE, Heine VM, van der Knaap MS
The Journal of clinical investigation126(4)1512-24(2016 Apr)

Eukaryotic initiation factor 2B epsilon induces cap-dependent translation and skeletal muscle hypertrophy.
Mayhew DL, Hornberger TA, Lincoln HC, Bamman MM
The Journal of physiology589(Pt 12)3023-37(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal a key role of the AKT-GSK3beta-eIF2Bepsilon signaling module in regulating axon regeneration in the adult mammalian central nervous system.
Title: GSK3? regulates AKT-induced central nervous system axon regeneration via an eIF2B?-dependent, mTORC1-independent pathway.

analysis of eukaryotic initiation factor 2B epsilon phosphorylation sites and ubiquitin-modified lysine residues
Title: Identification of ubiquitin-modified lysine residues and novel phosphorylation sites on eukaryotic initiation factor 2B epsilon.

Data show that toll-like receptors (TLRs)-TRIF signalling activates eIF2B GEF through PP2A-mediated serine dephosphorylation of the eIF2B varepsilon-subunit.
Title: Toll-like receptor activation suppresses ER stress factor CHOP and translation inhibition through activation of eIF2B.

mouse model of EIF2B-related leukodystrophy; findings provide evidence that hypomorphic Eif2b5 alleles lead to delayed and abnormal development and maintenance of CNS white matter
Title: A mouse model for eukaryotic translation initiation factor 2B-leucodystrophy reveals abnormal development of brain white matter.

inactivation of glycogen synthase kinase 3beta (GSK3beta) by high glucose and high insulin induces increase in synthesis of laminin beta1 via activation of eIF2Bepsilon.
Title: Glycogen synthase kinase 3beta is a novel regulator of high glucose- and high insulin-induced extracellular matrix protein synthesis in renal proximal tubular epithelial cells.

The following Eif2b5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2b5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03261	NM_172265.2 Latest version!	Mus musculus eukaryotic translation initiation factor 2B, subunit 5 epsilon (Eif2b5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2b5 ( NM_172265.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_758469.1 Mus musculus eukaryotic translation initiation factor 2B, subunit 5 epsilon (Eif2b5), mRNA.

Price & Availability↑

CloneID	OMu03261
Clone ID Related Accession (Same CDS sequence)	NM_172265.2
Accession Version	NM_172265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	translation initiation factor eIF-2B subunit epsilon
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161756.3 and AK160985.1. On Aug 25, 2009 this sequence version replaced NM_172265.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145801.1, BC038620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCCA CTGCGGCGGT GCCTGGCGCT GCGGCCGGCC GAGCGAGCAA ACGCGGCGGC 
GGCGGCTCGG GCGGCGGGGG AACCCAAGGT GCGGAGGAGG AGCCGCCGCC GCCCCTCCAG 
GCAGTTCTAG TGGCCGATAG CTTCAACCGC CGCTTCTTCC CCATCTCCAA GGACCAGCCT 
CGGGTCCTCT TGCCCCTGGC CAACGTTGCG CTAATTGACT ATACTCTGGA GTTCCTGACT 
GCCACGGGTG TACAGGAAAC ATTTGTCTTT TGCTGCTGGA AAGCTGCTCA GATCAAAGAA 
CACTTACAGA AATCCAAGTG GTGCCATCCT ACGTCCCCCA ACGTTGTTCG GATAATCACA 
TCAGAGCTAT ACCGATCACT AGGAGACGTC CTCCGTGACG TCGATGCCAA GGCCTTGGTG 
CGCTCTGACT TTCTCTTGAT ATATGGAGAT GTCATCTCAA ATATCAATAT CTGCAGAGCC 
CTGGAGGAAC ACAGGTTAAG AAGGAAGCTA GAAAAAAATG TCTCTGTGAT GACAATGGTC 
TTCAAAGAGT CGTCACCCAG CCACCCTACA CGCTGCCATG AGGACAACGT GGTGATGGCT 
GTGGACAGCG CCACCAACAG GGTTCTTCAC TTCCAGAAGA CCCAAGGCCT CCGGCGCTTT 
TCCTTTCCAT TGAGCCTGTT CCAGGGCAGT GGAGACGGAG TGGAGATTCG ATATGATTTG 
TTGGACTGTC ACATCAGCAT CTGCTCTCCT CAGGTAGCTC AGCTCTTCAC AGACAATTTT 
GACTACCAAA CTCGAGATGA TTTTGTACGA GGCATATTAA TGAATGAGGA GGTCCTAGGA 
AACCAGATTC ACTTGCATGT GACAACTAGG GAATATGGTG CCCGGGTCTC CAACCTACAC 
ATGTACTCAG CTGTTTGTGC TGACGTCATC CGCCGATGGG TCTACCCTCT CACTCCAGAA 
GTAAACTTCA CTGACAGCAC CACCCAGAGC TACACTCATT CCCGACACAA CATCTACCGA 
GGGCCTGAAG TCAGCCTGGG CCATGGCAGC GTCCTGGAGG AAAATGTGCT TTTGGGAGCT 
GGCACTGTCA TCGGCAGCAA CTGCTCCATC ACCAACAGTG TCATCGGCCC TAACTGCCAT 
ATTGGTGATA ATGTGGTACT GGACCAGGCC TACCTGTGGC AGGGAGTTCG AGTGGCTGCT 
GGAGCACAAA TACACCAATC TCTGCTCTGT GACAGAGCTG AGGTCAAGGA GCGAGTCAAA 
CTGAAGCCAT ACTGTGTTCT CACTTCCCAG GTGGTAGTGG GCCCAGACAT CACGCTGCCG 
GAGGGCTCGG TGATCTCTTT GCATCCTCCA GATGCAGAGG AAGATGAAGA TGATGGCCAG 
TTTAGTGATG ATTCTGGAGC TGACCAAGAA AAGGAGAAAG TGAAGCTGAA AGGTTACAAT 
CCAGCAGAAG TTGGCCTTGA AGGCCAGGGA TACCTCTGGA AAGCTGAGGG TGTGAACTCG 
AAAGAAGATG AGGAGCTACG GCAGAGTCTC TGGGGACTCA TGATCAAGAC GGAGGAGGAA 
AGTGAGACTG AAAGTGAGGG AAGTGTGGAT CCTGAGGAGC TGGACAGCCG AGCAGGCTCC 
CCTCAGCTGG ATGACATCAG AGTTTTCCAG AACGAGGTCC TGGGAACGCT GCAGCGGGGC 
AGAGAGGAGA ACATCTCCTG TGAAAATCTA GTCCTGGAGA TCAACTCTCT CAAGCATGCC 
TACAACATTA GCCTAAAGGA AGTGATGCAG GTCCTGACCC TTGTAGTCCT GGAGTTCCCC 
CTGCAACAAG TCGATGGCCT GCTTGACCCA AACCGCTACT GTGCCTTGCT GCTTCCTCTG 
CTCAAAGCCT GGAGCCCTGT TTTAAGGAAC TACATAAAGC GTGCAGCTGA CCACTTGGAA 
GCATTGGCAG CCATTGAGGA CTTCTTCTTG GAACATGAAA CTCTCGTTAC TTCCATGGCC 
AAGGTCCTGA TGGCTTTCTA CCAGCTGGAG ATCTTGGCTG AGGAAACAAT CTTGAGCTGG 
TTCAGCCAAA GAGACACAAC TGACGAAGGC CAGCAGTTAC GGAAGAATCA ACAGCTGCAG 
AGGTTCATCC AGTGGCTGAG AGAGGCAGAA GAAGAGTCAT CCGAAGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_758469.1
CDS	98..2251
Translation	MAATAAVPGAAAGRASKRGGGGSGGGGTQGAEEEPPPPLQAVLVADSFNRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLQKSKWCHPTSPNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLIYGDVISNINICRALEEHRLRRKLEKNVSVMTMVFKESSPSHPTRCHEDNVVMAVDSATNRVLHFQKTQGLRRFSFPLSLFQGSGDGVEIRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGILMNEEVLGNQIHLHVTTREYGARVSNLHMYSAVCADVIRRWVYPLTPEVNFTDSTTQSYTHSRHNIYRGPEVSLGHGSVLEENVLLGAGTVIGSNCSITNSVIGPNCHIGDNVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVKLKPYCVLTSQVVVGPDITLPEGSVISLHPPDAEEDEDDGQFSDDSGADQEKEKVKLKGYNPAEVGLEGQGYLWKAEGVNSKEDEELRQSLWGLMIKTEEESETESEGSVDPEELDSRAGSPQLDDIRVFQNEVLGTLQRGREENISCENLVLEINSLKHAYNISLKEVMQVLTLVVLEFPLQQVDGLLDPNRYCALLLPLLKAWSPVLRNYIKRAADHLEALAAIEDFFLEHETLVTSMAKVLMAFYQLEILAEETILSWFSQRDTTDEGQQLRKNQQLQRFIQWLREAEEESSEDD

Target ORF information:

RefSeq Version	NM_172265.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 2B, subunit 5 epsilon (Eif2b5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172265.2

ORF Insert Sequence:

ATGGCAGCCA CTGCGGCGGT GCCTGGCGCT GCGGCCGGCC GAGCGAGCAA ACGCGGCGGC 
GGCGGCTCGG GCGGCGGGGG AACCCAAGGT GCGGAGGAGG AGCCGCCGCC GCCCCTCCAG 
GCAGTTCTAG TGGCCGATAG CTTCAACCGC CGCTTCTTCC CCATCTCCAA GGACCAGCCT 
CGGGTCCTCT TGCCCCTGGC CAACGTTGCG CTAATTGACT ATACTCTGGA GTTCCTGACT 
GCCACGGGTG TACAGGAAAC ATTTGTCTTT TGCTGCTGGA AAGCTGCTCA GATCAAAGAA 
CACTTACAGA AATCCAAGTG GTGCCATCCT ACGTCCCCCA ACGTTGTTCG GATAATCACA 
TCAGAGCTAT ACCGATCACT AGGAGACGTC CTCCGTGACG TCGATGCCAA GGCCTTGGTG 
CGCTCTGACT TTCTCTTGAT ATATGGAGAT GTCATCTCAA ATATCAATAT CTGCAGAGCC 
CTGGAGGAAC ACAGGTTAAG AAGGAAGCTA GAAAAAAATG TCTCTGTGAT GACAATGGTC 
TTCAAAGAGT CGTCACCCAG CCACCCTACA CGCTGCCATG AGGACAACGT GGTGATGGCT 
GTGGACAGCG CCACCAACAG GGTTCTTCAC TTCCAGAAGA CCCAAGGCCT CCGGCGCTTT 
TCCTTTCCAT TGAGCCTGTT CCAGGGCAGT GGAGACGGAG TGGAGATTCG ATATGATTTG 
TTGGACTGTC ACATCAGCAT CTGCTCTCCT CAGGTAGCTC AGCTCTTCAC AGACAATTTT 
GACTACCAAA CTCGAGATGA TTTTGTACGA GGCATATTAA TGAATGAGGA GGTCCTAGGA 
AACCAGATTC ACTTGCATGT GACAACTAGG GAATATGGTG CCCGGGTCTC CAACCTACAC 
ATGTACTCAG CTGTTTGTGC TGACGTCATC CGCCGATGGG TCTACCCTCT CACTCCAGAA 
GTAAACTTCA CTGACAGCAC CACCCAGAGC TACACTCATT CCCGACACAA CATCTACCGA 
GGGCCTGAAG TCAGCCTGGG CCATGGCAGC GTCCTGGAGG AAAATGTGCT TTTGGGAGCT 
GGCACTGTCA TCGGCAGCAA CTGCTCCATC ACCAACAGTG TCATCGGCCC TAACTGCCAT 
ATTGGTGATA ATGTGGTACT GGACCAGGCC TACCTGTGGC AGGGAGTTCG AGTGGCTGCT 
GGAGCACAAA TACACCAATC TCTGCTCTGT GACAGAGCTG AGGTCAAGGA GCGAGTCAAA 
CTGAAGCCAT ACTGTGTTCT CACTTCCCAG GTGGTAGTGG GCCCAGACAT CACGCTGCCG 
GAGGGCTCGG TGATCTCTTT GCATCCTCCA GATGCAGAGG AAGATGAAGA TGATGGCCAG 
TTTAGTGATG ATTCTGGAGC TGACCAAGAA AAGGAGAAAG TGAAGCTGAA AGGTTACAAT 
CCAGCAGAAG TTGGCCTTGA AGGCCAGGGA TACCTCTGGA AAGCTGAGGG TGTGAACTCG 
AAAGAAGATG AGGAGCTACG GCAGAGTCTC TGGGGACTCA TGATCAAGAC GGAGGAGGAA 
AGTGAGACTG AAAGTGAGGG AAGTGTGGAT CCTGAGGAGC TGGACAGCCG AGCAGGCTCC 
CCTCAGCTGG ATGACATCAG AGTTTTCCAG AACGAGGTCC TGGGAACGCT GCAGCGGGGC 
AGAGAGGAGA ACATCTCCTG TGAAAATCTA GTCCTGGAGA TCAACTCTCT CAAGCATGCC 
TACAACATTA GCCTAAAGGA AGTGATGCAG GTCCTGACCC TTGTAGTCCT GGAGTTCCCC 
CTGCAACAAG TCGATGGCCT GCTTGACCCA AACCGCTACT GTGCCTTGCT GCTTCCTCTG 
CTCAAAGCCT GGAGCCCTGT TTTAAGGAAC TACATAAAGC GTGCAGCTGA CCACTTGGAA 
GCATTGGCAG CCATTGAGGA CTTCTTCTTG GAACATGAAA CTCTCGTTAC TTCCATGGCC 
AAGGTCCTGA TGGCTTTCTA CCAGCTGGAG ATCTTGGCTG AGGAAACAAT CTTGAGCTGG 
TTCAGCCAAA GAGACACAAC TGACGAAGGC CAGCAGTTAC GGAAGAATCA ACAGCTGCAG 
AGGTTCATCC AGTGGCTGAG AGAGGCAGAA GAAGAGTCAT CCGAAGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Affected astrocytes in the spinal cord of the leukodystrophy vanishing white matter.
Glia(2017 Dec)
Leferink PS,Breeuwsma N,Bugiani M,van der Knaap MS,Heine VM

Mutant eIF2B leads to impaired mitochondrial oxidative phosphorylation in vanishing white matter disease.
Journal of neurochemistry141(5)694-707(2017 Jun)
Raini G,Sharet R,Herrero M,Atzmon A,Shenoy A,Geiger T,Elroy-Stein O

Astrocytes are central in the pathomechanisms of vanishing white matter.
The Journal of clinical investigation126(4)1512-24(2016 Apr)
Dooves S,Bugiani M,Postma NL,Polder E,Land N,Horan ST,van Deijk AL,van de Kreeke A,Jacobs G,Vuong C,Klooster J,Kamermans M,Wortel J,Loos M,Wisse LE,Scheper GC,Abbink TE,Heine VM,van der Knaap MS

Eukaryotic initiation factor 2B epsilon induces cap-dependent translation and skeletal muscle hypertrophy.
The Journal of physiology589(Pt 12)3023-37(2011 Jun)
Mayhew DL,Hornberger TA,Lincoln HC,Bamman MM

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Eif2b5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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