Eif2b5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif2b5
Entrez Gene ID	192234
Full Name	eukaryotic translation initiation factor 2B subunit epsilon
General protein information	Preferred Names eukaryotic translation initiation factor 2B subunit epsilon Names translation initiation factor eIF-2B subunit epsilon eIF-2B GDP-GTP exchange factor subunit epsilon eukaryotic translation initiation factor 2B subunit 5 epsilon initiation factor eIF-2Be
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	subunit of eukaryotic initiation factor-2B, which plays a role in eukayrotic initiation factor 2 (eIF-2) recycling in translation initiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138866.2	NP_620221.2	translation initiation factor eIF-2B subunit epsilon

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2B5	XP_001139517.1
Gallus gallus (chicken)	EIF2B5	XP_422755.3
Drosophila melanogaster (fruit fly)	eIF2B-epsilon	NP_996329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000082	XP_001687758.1
Arabidopsis thaliana (thale cress)	AT2G34970	NP_181042.1
	EIF2B5	XP_001104122.1
Danio rerio (zebrafish)	eif2b5	NP_001107069.1
Schizosaccharomyces pombe (fission yeast)	tif225	NP_594285.1
Arabidopsis thaliana (thale cress)	AT4G18300	NP_193564.1
Xenopus tropicalis (tropical clawed frog)	eif2b5	XP_002935185.2
Mus musculus (house mouse)	Eif2b5	NP_758469.1
Saccharomyces cerevisiae (baker's yeast)	GCD6	NP_010497.3
Eremothecium gossypii	AGOS_AGL094W	NP_986572.1
Canis lupus familiaris (dog)	EIF2B5	XP_003640192.1
Bos taurus (cattle)	EIF2B5	XP_002684924.1
Rattus norvegicus (Norway rat)	Eif2b5	NP_620221.2
Kluyveromyces lactis	KLLA0A04235g	XP_451187.1
Neurospora crassa	NCU02414	XP_959596.1
Homo sapiens (human)	EIF2B5	NP_003898.2
Magnaporthe oryzae (rice blast fungus)	MGG_03517	XP_003716402.1
Arabidopsis thaliana (thale cress)	AT3G02270	NP_186876.1
Oryza sativa (rice)	Os02g0812400	NP_001048478.1

Gene Ontology
Bibliography

Related articles in PubMed

The mechanism of eukaryotic translation initiation: new insights and challenges.
Hinnebusch AG, Lorsch JR
Cold Spring Harbor perspectives in biology4(10)(2012 Oct)

Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.
Tuckow AP, Vary TC, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism299(2)E241-8(2010 Aug)

A point mutation in translation initiation factor 2B leads to a continuous hyper stress state in oligodendroglial-derived cells.
Kantor L, Pinchasi D, Mintz M, Hathout Y, Vanderver A, Elroy-Stein O
PloS one3(11)e3783(2008)

Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis.
Kubica N, Crispino JL, Gallagher JW, Kimball SR, Jefferson LS
The international journal of biochemistry & cell biology40(11)2522-33(2008)

Reduced eIF2alpha phosphorylation and increased proapoptotic proteins in aging.
Hussain SG, Ramaiah KV
Biochemical and biophysical research communications355(2)365-70(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

modulation of eIF2Bepsilon expression may be sufficient to correct deficits in skeletal muscle protein synthesis associated with sepsis and other muscle-wasting conditions
Title: Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.

oligodendroglial-derived cells expressing a mutated eIF2B5 constantly use their stress response mechanism as an adaptation mean in order to survive
Title: A point mutation in translation initiation factor 2B leads to a continuous hyper stress state in oligodendroglial-derived cells.

Data show that activation of mTORC1 is necessary and sufficient to stimulate eIF2Bepsilon mRNA translation.
Title: Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis.

mTOR signaling and eIF2Bepsilon mRNA translation are linked and could have roles in the stimulation of protein synthesis following acute resistance exercise
Title: Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.

The following Eif2b5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2b5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01807	NM_138866.2 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 2B subunit epsilon (Eif2b5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2b5 ( NM_138866.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620221.2 Rattus norvegicus eukaryotic translation initiation factor 2B subunit epsilon (Eif2b5), mRNA.

Price & Availability↑

CloneID	ORa01807
Clone ID Related Accession (Same CDS sequence)	NM_138866.2
Accession Version	NM_138866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	translation initiation factor eIF-2B subunit epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085698.1. On Nov 15, 2004 this sequence version replaced NM_138866.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085698.1, U19516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCCA CTGCGGCGGT TCCCAGCGCT GTAGGCGGCC GAGCGAACAA ACGCGGCGGC 
GGCTCCGGCG GCGGTGGAAC CCAAGGTGCG GAGGAGGAGC CGCCGCCGCC CCTCCAGGCA 
GTTTTAGTGG CCGATAGCTT CGACCGCCGC TTCTTCCCCA TCTCCAAGGA CCAGCCTCGG 
GTCCTATTGC CCCTGGCTAA TGTCGCGCTG ATCGACTATA CTCTGGAATT CTTGACTGCC 
ACGGGTGTAC AGGAAACATT TGTCTTTTGC TGCTGGAAGG CTGCTCAGAT CAAAGAACAC 
TTACAGAAAT CCAAATGGTG CCATCCTACG TCCCTCAATG TTGTTCGAAT AATCACATCA 
GACCTGTACC GATCACTAGG AGATGTCCTC CGTGATGTCG ATGCCAAGGC CTTGGTGCGC 
TCTGATTTCC TCCTGATATA TGGAGATGTC GTCTCAAATA TCAATATTTC CAAAGCCCTG 
GAGGAACACA GGTTAAGAAG GAAGCTAGAA AAAAATGTGT CTGTCATGAC AATGGTGTTC 
AAAGAGTCCT CACCCAGTCA CCCTACACGC TGCCATGAGG ACAATGTGGT GCTAGCTGTG 
GATAGCACCA CAAACAGGAT TCTTCACTTC CAGAAGACCC AAGGCCTCCG CCATTTCTCC 
TTTCCTTTGG GCCTATTCCA GGGCAGTCTA GACGGAGTGG AGATTCGATA TGACTTGTTG 
GATTGTCACA TCAGCATCTG CTCTCCTCAG GTAGCTCAGC TCTTCACAGA CAACTTTGAC 
TACCAAACTC GAGATGATTT TGTGCGAGGC CTGCTAGTGA ATGAGGAGAT CCTAGGAAAT 
CAGATTCACT TGCATGTGAC AAGTAGGGAA TATGGTGCCC GGGTCTCCAA CCTACACATG 
TACTCAGCTG TCTGTGCTGA CGTCATCCGC CGATGGGTCT ACCCTCTTAC TCCAGAAGTC 
AACTTCACTG ACAGCAGTAC ACAAAGCTAC ACTCATTCCC GACACAACAT CTACCGAGGG 
CCTGAAGTCA GCCTGGGCCA CGGCAGCGTC CTGGAGGAAA ACGTGCTTCT GGGAGCTGGC 
ACTGTCGTTG GCAGCAACTG CTCCATCACC AACAGTGTCA TTGGCCCTAA CTGCCACATT 
GGTGATAATG TGGTACTGGA CCAGGCCTAC CTGTGGCAGG GAGTTCGAGT GGCTGCTGGA 
GCACAAATAC ACCAGTCTCT GCTCTGTGAC AGAGCTGAGG TCAAGGAGCG AGTCATACTG 
AAGCCACACT GTGTCCTCAC TTCCCAGGTG GTAGTGGGCC CAGACATCAT ACTGCCCGAG 
GGCTCGGTGA TCTCTTTGCA CCCTCCAGAT GCAGAGGAAG ATGAAGATGA TGGCCAGTTT 
AGTGATGATT CTGGCGCTGA CCAAGAAAAG GAGAAAGTGA AGCTGAAAGG TTACAATCCG 
GCAGAAGTTG GCCCTGAAGG CCAGGGATAC CTCTGGAAAG CTGAGGATGT GGATGAGAAG 
GAAGATGAAG AGCTGCGGCA GAGTCTCTGG GGACTCATGA TCAATATGGA GGAGGAAAGT 
GAGACGGAAA GTGAGCGGAG CGTGGATCCC GAGGAGCTGG ACAGCCGAGC AGGCTCCCCT 
CAGCTGGATG ACATCAGAGT TTTCCAGAAC GAGGTCTTAG GAACGCTGCA GCGGGGCAGA 
GAGGAGAACA TCTCCTGTGA CAATCTAGTC CTGGAGATCA ACTCCCTCAA GTATGCCTAC 
AACATTAGCC TAAAGGAAGT GATGCAGGTC CTGAGCCATG TGGTCCTGGA GTTCCCCCTG 
CAACAAGTAG ATGGCGTGCT TGACCCAAAC CGCTACTGTG CCCTGCTGCT TCCTCTGCTC 
AAAGCCTGGA GCCCTGTTTT TCGGAACTAC ATAAAGCGCG CAGCTGACCA TTTGGAAGCA 
TTGGCGGCCA TTGAGGACTT CTTCTTGGAA CATGAAACTC TTGTTCCTTC CCTGGCCAAG 
GTCCTGATGG CTTTCTACCA GCTGGAGATC TTGGCTGAGG AAACAATCTT GAGCTGGTTC 
AGCCAAAGAG ACATAACTGA CAAAGGCCAG CAGTTACGTA AGAATCAACA GCTGCAGAGG 
TTCATCCAGT GGCTGAGAGA GGCAGAAGAG GAGTCATCTG ACGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620221.2
CDS	37..2187
Translation	MAATAAVPSAVGGRANKRGGGSGGGGTQGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLQKSKWCHPTSLNVVRIITSDLYRSLGDVLRDVDAKALVRSDFLLIYGDVVSNINISKALEEHRLRRKLEKNVSVMTMVFKESSPSHPTRCHEDNVVLAVDSTTNRILHFQKTQGLRHFSFPLGLFQGSLDGVEIRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHLHVTSREYGARVSNLHMYSAVCADVIRRWVYPLTPEVNFTDSSTQSYTHSRHNIYRGPEVSLGHGSVLEENVLLGAGTVVGSNCSITNSVIGPNCHIGDNVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVILKPHCVLTSQVVVGPDIILPEGSVISLHPPDAEEDEDDGQFSDDSGADQEKEKVKLKGYNPAEVGPEGQGYLWKAEDVDEKEDEELRQSLWGLMINMEEESETESERSVDPEELDSRAGSPQLDDIRVFQNEVLGTLQRGREENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQVDGVLDPNRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHETLVPSLAKVLMAFYQLEILAEETILSWFSQRDITDKGQQLRKNQQLQRFIQWLREAEEESSDDD

Target ORF information:

RefSeq Version	NM_138866.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 2B subunit epsilon (Eif2b5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138866.2

ORF Insert Sequence:

ATGGCAGCCA CTGCGGCGGT TCCCAGCGCT GTAGGCGGCC GAGCGAACAA ACGCGGCGGC 
GGCTCCGGCG GCGGTGGAAC CCAAGGTGCG GAGGAGGAGC CGCCGCCGCC CCTCCAGGCA 
GTTTTAGTGG CCGATAGCTT CGACCGCCGC TTCTTCCCCA TCTCCAAGGA CCAGCCTCGG 
GTCCTATTGC CCCTGGCTAA TGTCGCGCTG ATCGACTATA CTCTGGAATT CTTGACTGCC 
ACGGGTGTAC AGGAAACATT TGTCTTTTGC TGCTGGAAGG CTGCTCAGAT CAAAGAACAC 
TTACAGAAAT CCAAATGGTG CCATCCTACG TCCCTCAATG TTGTTCGAAT AATCACATCA 
GACCTGTACC GATCACTAGG AGATGTCCTC CGTGATGTCG ATGCCAAGGC CTTGGTGCGC 
TCTGATTTCC TCCTGATATA TGGAGATGTC GTCTCAAATA TCAATATTTC CAAAGCCCTG 
GAGGAACACA GGTTAAGAAG GAAGCTAGAA AAAAATGTGT CTGTCATGAC AATGGTGTTC 
AAAGAGTCCT CACCCAGTCA CCCTACACGC TGCCATGAGG ACAATGTGGT GCTAGCTGTG 
GATAGCACCA CAAACAGGAT TCTTCACTTC CAGAAGACCC AAGGCCTCCG CCATTTCTCC 
TTTCCTTTGG GCCTATTCCA GGGCAGTCTA GACGGAGTGG AGATTCGATA TGACTTGTTG 
GATTGTCACA TCAGCATCTG CTCTCCTCAG GTAGCTCAGC TCTTCACAGA CAACTTTGAC 
TACCAAACTC GAGATGATTT TGTGCGAGGC CTGCTAGTGA ATGAGGAGAT CCTAGGAAAT 
CAGATTCACT TGCATGTGAC AAGTAGGGAA TATGGTGCCC GGGTCTCCAA CCTACACATG 
TACTCAGCTG TCTGTGCTGA CGTCATCCGC CGATGGGTCT ACCCTCTTAC TCCAGAAGTC 
AACTTCACTG ACAGCAGTAC ACAAAGCTAC ACTCATTCCC GACACAACAT CTACCGAGGG 
CCTGAAGTCA GCCTGGGCCA CGGCAGCGTC CTGGAGGAAA ACGTGCTTCT GGGAGCTGGC 
ACTGTCGTTG GCAGCAACTG CTCCATCACC AACAGTGTCA TTGGCCCTAA CTGCCACATT 
GGTGATAATG TGGTACTGGA CCAGGCCTAC CTGTGGCAGG GAGTTCGAGT GGCTGCTGGA 
GCACAAATAC ACCAGTCTCT GCTCTGTGAC AGAGCTGAGG TCAAGGAGCG AGTCATACTG 
AAGCCACACT GTGTCCTCAC TTCCCAGGTG GTAGTGGGCC CAGACATCAT ACTGCCCGAG 
GGCTCGGTGA TCTCTTTGCA CCCTCCAGAT GCAGAGGAAG ATGAAGATGA TGGCCAGTTT 
AGTGATGATT CTGGCGCTGA CCAAGAAAAG GAGAAAGTGA AGCTGAAAGG TTACAATCCG 
GCAGAAGTTG GCCCTGAAGG CCAGGGATAC CTCTGGAAAG CTGAGGATGT GGATGAGAAG 
GAAGATGAAG AGCTGCGGCA GAGTCTCTGG GGACTCATGA TCAATATGGA GGAGGAAAGT 
GAGACGGAAA GTGAGCGGAG CGTGGATCCC GAGGAGCTGG ACAGCCGAGC AGGCTCCCCT 
CAGCTGGATG ACATCAGAGT TTTCCAGAAC GAGGTCTTAG GAACGCTGCA GCGGGGCAGA 
GAGGAGAACA TCTCCTGTGA CAATCTAGTC CTGGAGATCA ACTCCCTCAA GTATGCCTAC 
AACATTAGCC TAAAGGAAGT GATGCAGGTC CTGAGCCATG TGGTCCTGGA GTTCCCCCTG 
CAACAAGTAG ATGGCGTGCT TGACCCAAAC CGCTACTGTG CCCTGCTGCT TCCTCTGCTC 
AAAGCCTGGA GCCCTGTTTT TCGGAACTAC ATAAAGCGCG CAGCTGACCA TTTGGAAGCA 
TTGGCGGCCA TTGAGGACTT CTTCTTGGAA CATGAAACTC TTGTTCCTTC CCTGGCCAAG 
GTCCTGATGG CTTTCTACCA GCTGGAGATC TTGGCTGAGG AAACAATCTT GAGCTGGTTC 
AGCCAAAGAG ACATAACTGA CAAAGGCCAG CAGTTACGTA AGAATCAACA GCTGCAGAGG 
TTCATCCAGT GGCTGAGAGA GGCAGAAGAG GAGTCATCTG ACGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mechanism of eukaryotic translation initiation: new insights and challenges.
Cold Spring Harbor perspectives in biology4(10)(2012 Oct)
Hinnebusch AG,Lorsch JR

Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.
American journal of physiology. Endocrinology and metabolism299(2)E241-8(2010 Aug)
Tuckow AP,Vary TC,Kimball SR,Jefferson LS

A point mutation in translation initiation factor 2B leads to a continuous hyper stress state in oligodendroglial-derived cells.
PloS one3(11)e3783(2008)
Kantor L,Pinchasi D,Mintz M,Hathout Y,Vanderver A,Elroy-Stein O

Activation of the mammalian target of rapamycin complex 1 is both necessary and sufficient to stimulate eukaryotic initiation factor 2Bvarepsilon mRNA translation and protein synthesis.
The international journal of biochemistry & cell biology40(11)2522-33(2008)
Kubica N,Crispino JL,Gallagher JW,Kimball SR,Jefferson LS

Reduced eIF2alpha phosphorylation and increased proapoptotic proteins in aging.
Biochemical and biophysical research communications355(2)365-70(2007 Apr)
Hussain SG,Ramaiah KV

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Eif2b5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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