eIF2Bepsilon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF2Bepsilon
Entrez Gene ID	31156
Full Name	CG3806 gene product from transcript CG3806-RB
Synonyms	CG3806,Dmel\CG3806,EG:86E4.1,eIF-2bepsilon,eIF2Bepsilon,elf2B-epsilon
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B16-2B16

mRNA and Protein(s)

mRNA	Protein	Name
NM_206606.3	NP_996329.1	eukaryotic translation initiation factor 2B subunit epsilon, isoform B
NM_130605.5	NP_569961.2	eukaryotic translation initiation factor 2B subunit epsilon, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2B5	XP_001139517.1
Gallus gallus (chicken)	EIF2B5	XP_422755.3
Drosophila melanogaster (fruit fly)	eIF2B-epsilon	NP_996329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000082	XP_001687758.1
Arabidopsis thaliana (thale cress)	AT2G34970	NP_181042.1
	EIF2B5	XP_001104122.1
Danio rerio (zebrafish)	eif2b5	NP_001107069.1
Schizosaccharomyces pombe (fission yeast)	tif225	NP_594285.1
Arabidopsis thaliana (thale cress)	AT4G18300	NP_193564.1
Xenopus tropicalis (tropical clawed frog)	eif2b5	XP_002935185.2
Mus musculus (house mouse)	Eif2b5	NP_758469.1
Saccharomyces cerevisiae (baker's yeast)	GCD6	NP_010497.3
Eremothecium gossypii	AGOS_AGL094W	NP_986572.1
Canis lupus familiaris (dog)	EIF2B5	XP_003640192.1
Bos taurus (cattle)	EIF2B5	XP_002684924.1
Rattus norvegicus (Norway rat)	Eif2b5	NP_620221.2
Kluyveromyces lactis	KLLA0A04235g	XP_451187.1
Neurospora crassa	NCU02414	XP_959596.1
Homo sapiens (human)	EIF2B5	NP_003898.2
Magnaporthe oryzae (rice blast fungus)	MGG_03517	XP_003716402.1
Arabidopsis thaliana (thale cress)	AT3G02270	NP_186876.1
Oryza sativa (rice)	Os02g0812400	NP_001048478.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eIF2Bepsilon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF2Bepsilon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00533	NM_206606.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00533	NM_130605.5 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF2Bepsilon ( NM_206606.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996329.1 Drosophila melanogaster eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00533
Clone ID Related Accession (Same CDS sequence)	NM_206606.3 , NM_130605.5
Accession Version	NM_206606.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2B subunit epsilon, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206606.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCAAT TCGAGAAGGA GATCGTTCAG GCGGTGCTGA TCGCCGACAA CAATGTGTGG 
AACTTCAAGC CCCTCTCCGA CGAGGGCTCT ACGGCCCTGC TGCCGCTGGT CAACGTGCGG 
ATGCTTGACT ACGCTCTCAT GGCGCTGAAC CGCAGCGGCG TGGAGGAGGT GTTCGTGTAC 
ACCAGCCTCT ATCGCTCGAG CATCCGGGAG CACATCAGGG CCGGCATTGC CACGGACGCA 
GCCTGGTCAT TCAAGATGAC CGTCCATGTG ATTGGCGGCG AGGGTTGTCG CTGCTTCGGC 
GATGCCATGC GTGATTTGGA CAATAAGGCG CTGATCCGTG GCCACTTCAT TCTGCTCGGC 
GCCGATACGG TGACCAATGC CGATCTGCGC CCAGTGCTGG AGCAGCACAA GCGGCAGGCC 
AAGTTCGACA AGGGCACGGC CGCCACGCTG GTGTTCAAGG AGTGTGCCAA CAATGTGCGC 
ACTGGCAACG AGGTGCTGAT CGCCGTGGAC CGTCGCAACG ACCGGCTGCA CTACCACCAG 
CGGCTGCGGA TGCACCACAA GGAGAATCGC TACCAACTAC CACTAGATGT CTTTCTGGGC 
AACTCGTGTG TGGCGCTGCA CCACAACCTC CTAGATCCGC AGATTGCCAT TGGCTCGCCC 
TCAATGCTGT CCCTGTTCAA CGACAACTTT GACTTTCAGA CGCGTGATGA CTTCGTCAGG 
GGTTTGTTGA TAAACGAGGA ACTGCTGGAC AGCAGGATAT ATGTGGCACT GCTCCCGGCA 
GCCCAGTACG CGCACAAGGT GAACAACTGG CCGGCCTACC AGCTGGTTAG TCGGGACATT 
ATCAACCGGT GGGCCTACCC CTTCGTTCCG GACATAGGCC TTTACAAGCT GCAGCAGGAG 
TACGTCTTCC ACAAGGACAA CATCTACAAG AGCCCCGAGG CGCATGTCTC GAAAGTGGCT 
TTGCTCCAGA ACGTGGTCAT CGAAGCGGGC AGTCACGTAG ACAGTGGCTC AGTGATTAGT 
GACAGCGTGA TTGGCGCCAA TTGCCGGATC GGCAAGAACT GTCGACTGAC CAATGCGTTC 
CTCATGGCCG ACGTCACCGT AATGGACAAC TGCAGACTGG AGCACTGCGT GGTGGGCGAA 
GGAGCCATCA TCAACGAGGA TTGCGACGTG AGCGCGGGCT GTGTGCTCGG TGCTAAAAGC 
GTGCTGCCTG CCAAGAAAAC GCTGGCGAAG ACCCTTATCA CCAGAACGCC CAGCAGACGG 
CGTAGCGATG ATGAGGTGGT AGTGGAGCTG AAGTCCATCG GTCCGCATGC CTATATTGTC 
AGCGATTTGA CGACGGGTGA TCCCGAGGAC TCGGAGTGCG AGGACTTGTT GCCACAGCAG 
CCGCTCAGTA TACCCAAAAT GGGAGACTTG CTGGCGCCGC TGGACGAGAT CAGTTACTGC 
AGCGATTTTA GCGAGGACGA CGAGGATGCC TCGCGAGCGG TAACGCCACT GCCCGATGAC 
ACAAACATTT TCCTGGGCGA AGTCATCGAT TCCTTGACAC GGGGCTTCCG GGAGAAGTCC 
AACCCTGACT TCCTTGTCCT AGAAATCAAC TCCTCGCGCT ATGCGTACAA CATGTCCCTC 
AAAGAAGTCA ACTTCAACGT GGTGAAGGCC GTTTTTGGCA TGCAGAGCAT CGTGGAACCG 
GCCAACGACA ACGTACTAGT GGCCATAAAC GCAGCCTTTA AACAACTGGG CCCTGTGGTA 
TCCAACTACA TTAAGAGCGA GGATTCCATG ATGGATTGTC TCAAGGCGCT GGAGGATGTG 
TACGAGGAGA ATCATCTAGT GCGGGGGAAG ATCTCGCAGA TCGTGCACTA TCTGTACGAC 
AAGGACTTTG TGTCCGAGAG TGCCATTCAG GCGTGGTACG CGCAGTTGGA CGAGGAGGAG 
CATGCACACC TGCGCCAAAG CCTCGCCAAG CTCGTGGCTT GGTTGGATCA ATCCAGCGAA 
GAAGATGATG ACGAGGAGGA CGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996329.1
CDS	52..2061
Translation	MAQFEKEIVQAVLIADNNVWNFKPLSDEGSTALLPLVNVRMLDYALMALNRSGVEEVFVYTSLYRSSIREHIRAGIATDAAWSFKMTVHVIGGEGCRCFGDAMRDLDNKALIRGHFILLGADTVTNADLRPVLEQHKRQAKFDKGTAATLVFKECANNVRTGNEVLIAVDRRNDRLHYHQRLRMHHKENRYQLPLDVFLGNSCVALHHNLLDPQIAIGSPSMLSLFNDNFDFQTRDDFVRGLLINEELLDSRIYVALLPAAQYAHKVNNWPAYQLVSRDIINRWAYPFVPDIGLYKLQQEYVFHKDNIYKSPEAHVSKVALLQNVVIEAGSHVDSGSVISDSVIGANCRIGKNCRLTNAFLMADVTVMDNCRLEHCVVGEGAIINEDCDVSAGCVLGAKSVLPAKKTLAKTLITRTPSRRRSDDEVVVELKSIGPHAYIVSDLTTGDPEDSECEDLLPQQPLSIPKMGDLLAPLDEISYCSDFSEDDEDASRAVTPLPDDTNIFLGEVIDSLTRGFREKSNPDFLVLEINSSRYAYNMSLKEVNFNVVKAVFGMQSIVEPANDNVLVAINAAFKQLGPVVSNYIKSEDSMMDCLKALEDVYEENHLVRGKISQIVHYLYDKDFVSESAIQAWYAQLDEEEHAHLRQSLAKLVAWLDQSSEEDDDEEDDD

Target ORF information:

RefSeq Version	NM_206606.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206606.3

ORF Insert Sequence:

ATGGCCCAAT TCGAGAAGGA GATCGTTCAG GCGGTGCTGA TCGCCGACAA CAATGTGTGG 
AACTTCAAGC CCCTCTCCGA CGAGGGCTCT ACGGCCCTGC TGCCGCTGGT CAACGTGCGG 
ATGCTTGACT ACGCTCTCAT GGCGCTGAAC CGCAGCGGCG TGGAGGAGGT GTTCGTGTAC 
ACCAGCCTCT ATCGCTCGAG CATCCGGGAG CACATCAGGG CCGGCATTGC CACGGACGCA 
GCCTGGTCAT TCAAGATGAC CGTCCATGTG ATTGGCGGCG AGGGTTGTCG CTGCTTCGGC 
GATGCCATGC GTGATTTGGA CAATAAGGCG CTGATCCGTG GCCACTTCAT TCTGCTCGGC 
GCCGATACGG TGACCAATGC CGATCTGCGC CCAGTGCTGG AGCAGCACAA GCGGCAGGCC 
AAGTTCGACA AGGGCACGGC CGCCACGCTG GTGTTCAAGG AGTGTGCCAA CAATGTGCGC 
ACTGGCAACG AGGTGCTGAT CGCCGTGGAC CGTCGCAACG ACCGGCTGCA CTACCACCAG 
CGGCTGCGGA TGCACCACAA GGAGAATCGC TACCAACTAC CACTAGATGT CTTTCTGGGC 
AACTCGTGTG TGGCGCTGCA CCACAACCTC CTAGATCCGC AGATTGCCAT TGGCTCGCCC 
TCAATGCTGT CCCTGTTCAA CGACAACTTT GACTTTCAGA CGCGTGATGA CTTCGTCAGG 
GGTTTGTTGA TAAACGAGGA ACTGCTGGAC AGCAGGATAT ATGTGGCACT GCTCCCGGCA 
GCCCAGTACG CGCACAAGGT GAACAACTGG CCGGCCTACC AGCTGGTTAG TCGGGACATT 
ATCAACCGGT GGGCCTACCC CTTCGTTCCG GACATAGGCC TTTACAAGCT GCAGCAGGAG 
TACGTCTTCC ACAAGGACAA CATCTACAAG AGCCCCGAGG CGCATGTCTC GAAAGTGGCT 
TTGCTCCAGA ACGTGGTCAT CGAAGCGGGC AGTCACGTAG ACAGTGGCTC AGTGATTAGT 
GACAGCGTGA TTGGCGCCAA TTGCCGGATC GGCAAGAACT GTCGACTGAC CAATGCGTTC 
CTCATGGCCG ACGTCACCGT AATGGACAAC TGCAGACTGG AGCACTGCGT GGTGGGCGAA 
GGAGCCATCA TCAACGAGGA TTGCGACGTG AGCGCGGGCT GTGTGCTCGG TGCTAAAAGC 
GTGCTGCCTG CCAAGAAAAC GCTGGCGAAG ACCCTTATCA CCAGAACGCC CAGCAGACGG 
CGTAGCGATG ATGAGGTGGT AGTGGAGCTG AAGTCCATCG GTCCGCATGC CTATATTGTC 
AGCGATTTGA CGACGGGTGA TCCCGAGGAC TCGGAGTGCG AGGACTTGTT GCCACAGCAG 
CCGCTCAGTA TACCCAAAAT GGGAGACTTG CTGGCGCCGC TGGACGAGAT CAGTTACTGC 
AGCGATTTTA GCGAGGACGA CGAGGATGCC TCGCGAGCGG TAACGCCACT GCCCGATGAC 
ACAAACATTT TCCTGGGCGA AGTCATCGAT TCCTTGACAC GGGGCTTCCG GGAGAAGTCC 
AACCCTGACT TCCTTGTCCT AGAAATCAAC TCCTCGCGCT ATGCGTACAA CATGTCCCTC 
AAAGAAGTCA ACTTCAACGT GGTGAAGGCC GTTTTTGGCA TGCAGAGCAT CGTGGAACCG 
GCCAACGACA ACGTACTAGT GGCCATAAAC GCAGCCTTTA AACAACTGGG CCCTGTGGTA 
TCCAACTACA TTAAGAGCGA GGATTCCATG ATGGATTGTC TCAAGGCGCT GGAGGATGTG 
TACGAGGAGA ATCATCTAGT GCGGGGGAAG ATCTCGCAGA TCGTGCACTA TCTGTACGAC 
AAGGACTTTG TGTCCGAGAG TGCCATTCAG GCGTGGTACG CGCAGTTGGA CGAGGAGGAG 
CATGCACACC TGCGCCAAAG CCTCGCCAAG CTCGTGGCTT GGTTGGATCA ATCCAGCGAA 
GAAGATGATG ACGAGGAGGA CGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF2Bepsilon ( NM_130605.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569961.2 Drosophila melanogaster eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00533
Clone ID Related Accession (Same CDS sequence)	NM_206606.3 , NM_130605.5
Accession Version	NM_130605.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2B subunit epsilon, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130605.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCAAT TCGAGAAGGA GATCGTTCAG GCGGTGCTGA TCGCCGACAA CAATGTGTGG 
AACTTCAAGC CCCTCTCCGA CGAGGGCTCT ACGGCCCTGC TGCCGCTGGT CAACGTGCGG 
ATGCTTGACT ACGCTCTCAT GGCGCTGAAC CGCAGCGGCG TGGAGGAGGT GTTCGTGTAC 
ACCAGCCTCT ATCGCTCGAG CATCCGGGAG CACATCAGGG CCGGCATTGC CACGGACGCA 
GCCTGGTCAT TCAAGATGAC CGTCCATGTG ATTGGCGGCG AGGGTTGTCG CTGCTTCGGC 
GATGCCATGC GTGATTTGGA CAATAAGGCG CTGATCCGTG GCCACTTCAT TCTGCTCGGC 
GCCGATACGG TGACCAATGC CGATCTGCGC CCAGTGCTGG AGCAGCACAA GCGGCAGGCC 
AAGTTCGACA AGGGCACGGC CGCCACGCTG GTGTTCAAGG AGTGTGCCAA CAATGTGCGC 
ACTGGCAACG AGGTGCTGAT CGCCGTGGAC CGTCGCAACG ACCGGCTGCA CTACCACCAG 
CGGCTGCGGA TGCACCACAA GGAGAATCGC TACCAACTAC CACTAGATGT CTTTCTGGGC 
AACTCGTGTG TGGCGCTGCA CCACAACCTC CTAGATCCGC AGATTGCCAT TGGCTCGCCC 
TCAATGCTGT CCCTGTTCAA CGACAACTTT GACTTTCAGA CGCGTGATGA CTTCGTCAGG 
GGTTTGTTGA TAAACGAGGA ACTGCTGGAC AGCAGGATAT ATGTGGCACT GCTCCCGGCA 
GCCCAGTACG CGCACAAGGT GAACAACTGG CCGGCCTACC AGCTGGTTAG TCGGGACATT 
ATCAACCGGT GGGCCTACCC CTTCGTTCCG GACATAGGCC TTTACAAGCT GCAGCAGGAG 
TACGTCTTCC ACAAGGACAA CATCTACAAG AGCCCCGAGG CGCATGTCTC GAAAGTGGCT 
TTGCTCCAGA ACGTGGTCAT CGAAGCGGGC AGTCACGTAG ACAGTGGCTC AGTGATTAGT 
GACAGCGTGA TTGGCGCCAA TTGCCGGATC GGCAAGAACT GTCGACTGAC CAATGCGTTC 
CTCATGGCCG ACGTCACCGT AATGGACAAC TGCAGACTGG AGCACTGCGT GGTGGGCGAA 
GGAGCCATCA TCAACGAGGA TTGCGACGTG AGCGCGGGCT GTGTGCTCGG TGCTAAAAGC 
GTGCTGCCTG CCAAGAAAAC GCTGGCGAAG ACCCTTATCA CCAGAACGCC CAGCAGACGG 
CGTAGCGATG ATGAGGTGGT AGTGGAGCTG AAGTCCATCG GTCCGCATGC CTATATTGTC 
AGCGATTTGA CGACGGGTGA TCCCGAGGAC TCGGAGTGCG AGGACTTGTT GCCACAGCAG 
CCGCTCAGTA TACCCAAAAT GGGAGACTTG CTGGCGCCGC TGGACGAGAT CAGTTACTGC 
AGCGATTTTA GCGAGGACGA CGAGGATGCC TCGCGAGCGG TAACGCCACT GCCCGATGAC 
ACAAACATTT TCCTGGGCGA AGTCATCGAT TCCTTGACAC GGGGCTTCCG GGAGAAGTCC 
AACCCTGACT TCCTTGTCCT AGAAATCAAC TCCTCGCGCT ATGCGTACAA CATGTCCCTC 
AAAGAAGTCA ACTTCAACGT GGTGAAGGCC GTTTTTGGCA TGCAGAGCAT CGTGGAACCG 
GCCAACGACA ACGTACTAGT GGCCATAAAC GCAGCCTTTA AACAACTGGG CCCTGTGGTA 
TCCAACTACA TTAAGAGCGA GGATTCCATG ATGGATTGTC TCAAGGCGCT GGAGGATGTG 
TACGAGGAGA ATCATCTAGT GCGGGGGAAG ATCTCGCAGA TCGTGCACTA TCTGTACGAC 
AAGGACTTTG TGTCCGAGAG TGCCATTCAG GCGTGGTACG CGCAGTTGGA CGAGGAGGAG 
CATGCACACC TGCGCCAAAG CCTCGCCAAG CTCGTGGCTT GGTTGGATCA ATCCAGCGAA 
GAAGATGATG ACGAGGAGGA CGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569961.2
CDS	52..2061
Translation	MAQFEKEIVQAVLIADNNVWNFKPLSDEGSTALLPLVNVRMLDYALMALNRSGVEEVFVYTSLYRSSIREHIRAGIATDAAWSFKMTVHVIGGEGCRCFGDAMRDLDNKALIRGHFILLGADTVTNADLRPVLEQHKRQAKFDKGTAATLVFKECANNVRTGNEVLIAVDRRNDRLHYHQRLRMHHKENRYQLPLDVFLGNSCVALHHNLLDPQIAIGSPSMLSLFNDNFDFQTRDDFVRGLLINEELLDSRIYVALLPAAQYAHKVNNWPAYQLVSRDIINRWAYPFVPDIGLYKLQQEYVFHKDNIYKSPEAHVSKVALLQNVVIEAGSHVDSGSVISDSVIGANCRIGKNCRLTNAFLMADVTVMDNCRLEHCVVGEGAIINEDCDVSAGCVLGAKSVLPAKKTLAKTLITRTPSRRRSDDEVVVELKSIGPHAYIVSDLTTGDPEDSECEDLLPQQPLSIPKMGDLLAPLDEISYCSDFSEDDEDASRAVTPLPDDTNIFLGEVIDSLTRGFREKSNPDFLVLEINSSRYAYNMSLKEVNFNVVKAVFGMQSIVEPANDNVLVAINAAFKQLGPVVSNYIKSEDSMMDCLKALEDVYEENHLVRGKISQIVHYLYDKDFVSESAIQAWYAQLDEEEHAHLRQSLAKLVAWLDQSSEEDDDEEDDD

Target ORF information:

RefSeq Version	NM_130605.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2B subunit epsilon (eIF2Bepsilon), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130605.5

ORF Insert Sequence:

ATGGCCCAAT TCGAGAAGGA GATCGTTCAG GCGGTGCTGA TCGCCGACAA CAATGTGTGG 
AACTTCAAGC CCCTCTCCGA CGAGGGCTCT ACGGCCCTGC TGCCGCTGGT CAACGTGCGG 
ATGCTTGACT ACGCTCTCAT GGCGCTGAAC CGCAGCGGCG TGGAGGAGGT GTTCGTGTAC 
ACCAGCCTCT ATCGCTCGAG CATCCGGGAG CACATCAGGG CCGGCATTGC CACGGACGCA 
GCCTGGTCAT TCAAGATGAC CGTCCATGTG ATTGGCGGCG AGGGTTGTCG CTGCTTCGGC 
GATGCCATGC GTGATTTGGA CAATAAGGCG CTGATCCGTG GCCACTTCAT TCTGCTCGGC 
GCCGATACGG TGACCAATGC CGATCTGCGC CCAGTGCTGG AGCAGCACAA GCGGCAGGCC 
AAGTTCGACA AGGGCACGGC CGCCACGCTG GTGTTCAAGG AGTGTGCCAA CAATGTGCGC 
ACTGGCAACG AGGTGCTGAT CGCCGTGGAC CGTCGCAACG ACCGGCTGCA CTACCACCAG 
CGGCTGCGGA TGCACCACAA GGAGAATCGC TACCAACTAC CACTAGATGT CTTTCTGGGC 
AACTCGTGTG TGGCGCTGCA CCACAACCTC CTAGATCCGC AGATTGCCAT TGGCTCGCCC 
TCAATGCTGT CCCTGTTCAA CGACAACTTT GACTTTCAGA CGCGTGATGA CTTCGTCAGG 
GGTTTGTTGA TAAACGAGGA ACTGCTGGAC AGCAGGATAT ATGTGGCACT GCTCCCGGCA 
GCCCAGTACG CGCACAAGGT GAACAACTGG CCGGCCTACC AGCTGGTTAG TCGGGACATT 
ATCAACCGGT GGGCCTACCC CTTCGTTCCG GACATAGGCC TTTACAAGCT GCAGCAGGAG 
TACGTCTTCC ACAAGGACAA CATCTACAAG AGCCCCGAGG CGCATGTCTC GAAAGTGGCT 
TTGCTCCAGA ACGTGGTCAT CGAAGCGGGC AGTCACGTAG ACAGTGGCTC AGTGATTAGT 
GACAGCGTGA TTGGCGCCAA TTGCCGGATC GGCAAGAACT GTCGACTGAC CAATGCGTTC 
CTCATGGCCG ACGTCACCGT AATGGACAAC TGCAGACTGG AGCACTGCGT GGTGGGCGAA 
GGAGCCATCA TCAACGAGGA TTGCGACGTG AGCGCGGGCT GTGTGCTCGG TGCTAAAAGC 
GTGCTGCCTG CCAAGAAAAC GCTGGCGAAG ACCCTTATCA CCAGAACGCC CAGCAGACGG 
CGTAGCGATG ATGAGGTGGT AGTGGAGCTG AAGTCCATCG GTCCGCATGC CTATATTGTC 
AGCGATTTGA CGACGGGTGA TCCCGAGGAC TCGGAGTGCG AGGACTTGTT GCCACAGCAG 
CCGCTCAGTA TACCCAAAAT GGGAGACTTG CTGGCGCCGC TGGACGAGAT CAGTTACTGC 
AGCGATTTTA GCGAGGACGA CGAGGATGCC TCGCGAGCGG TAACGCCACT GCCCGATGAC 
ACAAACATTT TCCTGGGCGA AGTCATCGAT TCCTTGACAC GGGGCTTCCG GGAGAAGTCC 
AACCCTGACT TCCTTGTCCT AGAAATCAAC TCCTCGCGCT ATGCGTACAA CATGTCCCTC 
AAAGAAGTCA ACTTCAACGT GGTGAAGGCC GTTTTTGGCA TGCAGAGCAT CGTGGAACCG 
GCCAACGACA ACGTACTAGT GGCCATAAAC GCAGCCTTTA AACAACTGGG CCCTGTGGTA 
TCCAACTACA TTAAGAGCGA GGATTCCATG ATGGATTGTC TCAAGGCGCT GGAGGATGTG 
TACGAGGAGA ATCATCTAGT GCGGGGGAAG ATCTCGCAGA TCGTGCACTA TCTGTACGAC 
AAGGACTTTG TGTCCGAGAG TGCCATTCAG GCGTGGTACG CGCAGTTGGA CGAGGAGGAG 
CATGCACACC TGCGCCAAAG CCTCGCCAAG CTCGTGGCTT GGTTGGATCA ATCCAGCGAA 
GAAGATGATG ACGAGGAGGA CGATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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eIF2Bepsilon cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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