SPBC3B8.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC3B8.05 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC3B8.05 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100494 NM_001022328.2
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Schizosaccharomyces pombe putative diphthamide biosynthesis protein Dph1 (SPBC3B8.05), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100494
    Clone ID Related Accession (Same CDS sequence) NM_001022328.2
    Accession Version NM_001022328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative diphthamide biosynthesis protein Dph1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022328.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGGAAG TGAAAAAATC AATTCCAAAA CGTCGTTTTG TTGGCAAAAA AAATAGGAAG 
    GAAAATAATT TGGATGGTTC TAATAGAGAT GTTGAAAATG CTGCTCTCGT CACTATTAAC
    TCCAAGAGGA GCGCTGGACG AGTAGCTACT CAAATCCCAG AGGATATATT AAACGATAAA
    GCAATTAATG AAGCTATAAA ATTGTTACCG CAAAATTACA ATTTTGAAAT TCACAAAACC
    ATTTGGCATA TTCGCTTACG AAAAGCAAAA CGTGTTGCCT TGCAACTTCC CGAGGGGCTA
    TTGATGTTTG GTTGTATATT GTCTGATATT TTTGAACAAT TTTGCCAGGT CGAGACAATT
    GTGATGGGCG ACGTTACATA CGGTGCGTGT TGTATTGACG ATTTTACAGC TAGGGCACTC
    GATTGCGATT TTTTAGTACA CTATGGTCAT TCTTGTCTTA TACCTGTTGA TCAAACGCCC
    ATAAAGGTGC TATATGTTTT CGTGGATATA AAGATAGATT TGCAACATGT CGTATCATCT
    TTAAAACATA ATCTACCCTC CAATTCTAGG TTAGCTTTGG TTGGAACTAT CCAATTTGTT
    GGATCTCTCA ACTCTATAAA AGACGCTTTG CAAATACAGG ATGAAGACGG AAAGGGAGGT
    TTTTACGTTG TTATTCCACA AGCAAAGCCA TTATCTCCTG GAGAGGCGTT GGGATGTACA
    TCTCCCTATA TTGAAAAAGG ATCTGTAGAT GCATTAATTT ACATTGGTGA CGGAAGATTC
    CATTTGGAAT CTGTCATGAT TGCTAATCCT GATTTGCCCG CATACCGCTA TGATCCTTAC
    TCACATAAGC TCTCTATTGA ATCTTATGCT CATGAAGAAA TGAAATCTAT TCGGTATTCC
    GCCGTTGAGA AAGCTCGTAC CGCTAAAAAG TTCGGTCTAA TTCAAGGTAC ACTGGGTCGA
    CAGGGGTCTC CTAAGGTACT CGAAAATTTA AAGAATACTT TAAGGAAAAA TAATAAAGAT
    TTCGTTTGCG TTTTAATGTC AGAAATTTTT CCTAGTAGAT TGGGTCAATT CTCTGATATT
    GACGCGTGGA TTCAGGTCGC TTGTCCAAGG CTTTCCATAG ATTGGGGCTA TGCATTTCCC
    GCTCCATTGT TGACACCATA TGAAGCATCT GCTGCTTTTA ATGTTGTTCC TTGGAAGGAA
    GTTTATCCTA TGGACTTTTA CGCTACCAAT TCATTAGGTA ATTGGACTCC AAATAATCCC
    GAAAATCGAC CTCTGCCAAA TCGCAAAAAA ACCGGACCCG TTTCTTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596409.1
    CDS142..1452
    Translation

    Target ORF information:

    RefSeq Version NM_001022328.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative diphthamide biosynthesis protein Dph1 (SPBC3B8.05), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCGGAAG TGAAAAAATC AATTCCAAAA CGTCGTTTTG TTGGCAAAAA AAATAGGAAG 
    GAAAATAATT TGGATGGTTC TAATAGAGAT GTTGAAAATG CTGCTCTCGT CACTATTAAC
    TCCAAGAGGA GCGCTGGACG AGTAGCTACT CAAATCCCAG AGGATATATT AAACGATAAA
    GCAATTAATG AAGCTATAAA ATTGTTACCG CAAAATTACA ATTTTGAAAT TCACAAAACC
    ATTTGGCATA TTCGCTTACG AAAAGCAAAA CGTGTTGCCT TGCAACTTCC CGAGGGGCTA
    TTGATGTTTG GTTGTATATT GTCTGATATT TTTGAACAAT TTTGCCAGGT CGAGACAATT
    GTGATGGGCG ACGTTACATA CGGTGCGTGT TGTATTGACG ATTTTACAGC TAGGGCACTC
    GATTGCGATT TTTTAGTACA CTATGGTCAT TCTTGTCTTA TACCTGTTGA TCAAACGCCC
    ATAAAGGTGC TATATGTTTT CGTGGATATA AAGATAGATT TGCAACATGT CGTATCATCT
    TTAAAACATA ATCTACCCTC CAATTCTAGG TTAGCTTTGG TTGGAACTAT CCAATTTGTT
    GGATCTCTCA ACTCTATAAA AGACGCTTTG CAAATACAGG ATGAAGACGG AAAGGGAGGT
    TTTTACGTTG TTATTCCACA AGCAAAGCCA TTATCTCCTG GAGAGGCGTT GGGATGTACA
    TCTCCCTATA TTGAAAAAGG ATCTGTAGAT GCATTAATTT ACATTGGTGA CGGAAGATTC
    CATTTGGAAT CTGTCATGAT TGCTAATCCT GATTTGCCCG CATACCGCTA TGATCCTTAC
    TCACATAAGC TCTCTATTGA ATCTTATGCT CATGAAGAAA TGAAATCTAT TCGGTATTCC
    GCCGTTGAGA AAGCTCGTAC CGCTAAAAAG TTCGGTCTAA TTCAAGGTAC ACTGGGTCGA
    CAGGGGTCTC CTAAGGTACT CGAAAATTTA AAGAATACTT TAAGGAAAAA TAATAAAGAT
    TTCGTTTGCG TTTTAATGTC AGAAATTTTT CCTAGTAGAT TGGGTCAATT CTCTGATATT
    GACGCGTGGA TTCAGGTCGC TTGTCCAAGG CTTTCCATAG ATTGGGGCTA TGCATTTCCC
    GCTCCATTGT TGACACCATA TGAAGCATCT GCTGCTTTTA ATGTTGTTCC TTGGAAGGAA
    GTTTATCCTA TGGACTTTTA CGCTACCAAT TCATTAGGTA ATTGGACTCC AAATAATCCC
    GAAAATCGAC CTCTGCCAAA TCGCAAAAAA ACCGGACCCG TTTCTTCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L