Dph1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dph1
Entrez Gene ID	116905
Full Name	diphthamide biosynthesis 1
Synonyms	2310011M22Rik,4930488F09Rik,AW551873,Dph2l1,Ovca1
General protein information	Preferred Names diphthamide biosynthesis 1 Names 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 DPH1 homolog DPH2-like 1 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1 diphthamide biosynthesis protein 1 diphthamide biosynthesis protein 2 homolog-like 1 diphtheria toxin resistance protein 1 diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1 mmDph1 ovarian cancer-associated gene 1 protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 45.76 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_144491.2	NP_652762.2	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DPH1	NP_001070367.1
Mus musculus (house mouse)	Dph1	NP_652762.2
Rattus norvegicus (Norway rat)	Dph1	NP_001099279.1
Danio rerio (zebrafish)	dph1	NP_001017861.1
Drosophila melanogaster (fruit fly)	CG11652	NP_648496.1
Xenopus tropicalis (tropical clawed frog)	dph1	NP_001135627.1
Homo sapiens (human)	DPH1	NP_001374.3
	LOC721275	XP_002808156.1
Gallus gallus (chicken)	DPH1	NP_001025887.1
Kluyveromyces lactis	KLLA0F03971g	XP_455260.1
Schizosaccharomyces pombe (fission yeast)	SPBC3B8.05	NP_596409.1
Saccharomyces cerevisiae (baker's yeast)	DPH1	NP_012163.1
Arabidopsis thaliana (thale cress)	AT5G62030	NP_201009.1
Pan troglodytes (chimpanzee)	DPH1	XP_511255.2
Neurospora crassa	NCU08503	XP_963432.1
Oryza sativa (rice)	Os02g0815600	NP_001048504.1
Caenorhabditis elegans (roundworm)	dph-1	NP_497750.1
Eremothecium gossypii	AGOS_ADL229W	NP_983867.1
Magnaporthe oryzae (rice blast fungus)	MGG_03237	XP_003716739.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of OVCA1/DPH1 in craniofacial abnormalities of Miller-Dieker syndrome.
Yu YR, You LR, Yan YT, Chen CM
Human molecular genetics23(21)5579-96(2014 Nov)

Diphthamide modification on eukaryotic elongation factor 2 is needed to assure fidelity of mRNA translation and mouse development.
Liu S, Bachran C, Gupta P, Miller-Randolph S, Wang H, Crown D, Zhang Y, Wein AN, Singh R, Fattah R, Leppla SH
Proceedings of the National Academy of Sciences of the United States of America109(34)13817-22(2012 Aug)

Ovca1, a candidate gene of the genetic modifier of Tp53, Mop2, affects mouse embryonic lethality.
Liang M, Ayanga B, Du S, Godwin AK, Hartsock JK, Evans SC
Genes, chromosomes & cancer47(4)315-25(2008 Apr)

Gene trap mutagenesis-based forward genetic approach reveals that the tumor suppressor OVCA1 is a component of the biosynthetic pathway of diphthamide on elongation factor 2.
Nobukuni Y, Kohno K, Miyagawa K
The Journal of biological chemistry280(11)10572-7(2005 Mar)

OVCA1: tumor suppressor gene.
Chen CM, Behringer RR
Current opinion in genetics & development15(1)49-54(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that OVCA1 deficiency in the neural crest contributes to the craniofacial abnormalities in patients with MDS.
Title: Role of OVCA1/DPH1 in craniofacial abnormalities of Miller-Dieker syndrome.

Ovca1 is a tumor suppressor that can modify p53-induced tumorigenesis and suggest that it acts as a positive regulator for cell cycle progression.
Title: Ovca1 regulates cell proliferation, embryonic development, and tumorigenesis.

The following Dph1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dph1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20741	NM_144491.2 Latest version!	Mus musculus diphthamide biosynthesis 1 (Dph1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dph1 ( NM_144491.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_652762.2 Mus musculus diphthamide biosynthesis 1 (Dph1), mRNA.

Price & Availability↑

CloneID	OMu20741
Clone ID Related Accession (Same CDS sequence)	NM_144491.2
Accession Version	NM_144491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Mus musculus(house mouse)
Product	2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB651982.1, AY078170.1 and AK082812.1. On Nov 16, 2006 this sequence version replaced NM_144491.1. ##Evidence-Data-START## Transcript exon combination :: AY078170.1, AK082812.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGC TGGTTGTGTC CGAGACTGCG GAGCCAGGAA GCCGAGTCGG CCCTGGCAGA 
GGTCGCATCT CTCGGGGGCG ACTGGCCAAT CAGATCCCCC CTGAGGTCCT GAACAACCCC 
CAGTTACAGG CTGCTGTCCA AGTTCTGCCT TCTAACTACA ACTTTGAGAT CCCCAAAACC 
ATCTGGAGAA TCCAGCAGGC CCAGGCCAAG AAGGTGGCCT TACAAATGCC AGAAGGCCTC 
CTCCTCTTTG CCTGCACTAT TGTGGATATC TTGGAAAGGT TCACAGAGGC TGAGGTGATG 
GTGATGGGTG ATGTCACCTA TGGGGCTTGC TGTGTGGATG ACTTCACTGC AAGGGCCTTG 
GGAGTTGACT TCCTGGTGCA CTATGGTCAC AGCTGTCTAG TCCCCATGGA CACCTCCGTT 
CAAGACTTCC GAGTCTTGTA TGTCTTCGTG GATATCCGGA TAGACACTGC CCACCTTCTG 
GACTCGGTCC GCCTCACCTT TACCCCAGGC AGCTCACTCG CTCTGGTCAG CACCATTCAG 
TTTGTGTCAA CCTTACAGGC AGCTGCACAG GAGCTGAAAG CTGATTATCA CATCAGTGTC 
CCACAGTGCA AGCCCCTGTC CCCTGGGGAG ATCCTAGGCT GCACATCCCC TCGGCTATCC 
AAGGAAGTGG AAGCTGTTGT GTATCTTGGA GATGGCCGCT TTCATCTGGA GTCTGTCATG 
ATTGCCAACC CTAATATACC TGCTTACCGG TATGACCCAT ATGGCAAAGT CCTATCCAGA 
GAATACTATG ACCATCAGCG CATGCAGGCC ACTCGCCAGG AAGCCATTGC TGCTGCACGC 
TCAGCCAAAT CCTGGGGCCT TATTCTGGGA ACCTTGGGCC GCCAGGGCAG TCCCAAGATC 
CTGGAGCACT TGGAATCTCA GCTCAGAAAC TTGGGACTTC CTTTCGTGAG GCTGTTGCTC 
TCTGAGATCT TCCCCAGCAA GCTCAGTCTA CTTCCTGAGG TGGATGTGTG GGTGCAGGTG 
GCATGTCCAC GCCTCTCCAT TGACTGGGGT TCAGCCTTTC CCAAGCCACT GCTGACACCG 
TACGAGGCAG CTGTGGCCCT GAAGGACATT TCTTGGCAGC AACCCTACCC CATGGACTTC 
TACTCTGGCA GCTCCTTAGG GCCATGGACA GTGAACTACG GTCGGGACCG AGCACCTCGG 
GGTCTCTGCC AGCCTGCATC TGACAAGGTG CAGCAAGGGT CCAGAGGCGG CTCTCCAGCC 
CCAGCCTGTG AGAGTTGCAA CTGCGCAGAC CAGAAGGCTA CTTCGCCGGC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652762.2
CDS	25..1341
Translation	MAALVVSETAEPGSRVGPGRGRISRGRLANQIPPEVLNNPQLQAAVQVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGVDFLVHYGHSCLVPMDTSVQDFRVLYVFVDIRIDTAHLLDSVRLTFTPGSSLALVSTIQFVSTLQAAAQELKADYHISVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNIPAYRYDPYGKVLSREYYDHQRMQATRQEAIAAARSAKSWGLILGTLGRQGSPKILEHLESQLRNLGLPFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGSAFPKPLLTPYEAAVALKDISWQQPYPMDFYSGSSLGPWTVNYGRDRAPRGLCQPASDKVQQGSRGGSPAPACESCNCADQKATSPAP

Target ORF information:

RefSeq Version	NM_144491.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diphthamide biosynthesis 1 (Dph1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144491.2

ORF Insert Sequence:

ATGGCGGCGC TGGTTGTGTC CGAGACTGCG GAGCCAGGAA GCCGAGTCGG CCCTGGCAGA 
GGTCGCATCT CTCGGGGGCG ACTGGCCAAT CAGATCCCCC CTGAGGTCCT GAACAACCCC 
CAGTTACAGG CTGCTGTCCA AGTTCTGCCT TCTAACTACA ACTTTGAGAT CCCCAAAACC 
ATCTGGAGAA TCCAGCAGGC CCAGGCCAAG AAGGTGGCCT TACAAATGCC AGAAGGCCTC 
CTCCTCTTTG CCTGCACTAT TGTGGATATC TTGGAAAGGT TCACAGAGGC TGAGGTGATG 
GTGATGGGTG ATGTCACCTA TGGGGCTTGC TGTGTGGATG ACTTCACTGC AAGGGCCTTG 
GGAGTTGACT TCCTGGTGCA CTATGGTCAC AGCTGTCTAG TCCCCATGGA CACCTCCGTT 
CAAGACTTCC GAGTCTTGTA TGTCTTCGTG GATATCCGGA TAGACACTGC CCACCTTCTG 
GACTCGGTCC GCCTCACCTT TACCCCAGGC AGCTCACTCG CTCTGGTCAG CACCATTCAG 
TTTGTGTCAA CCTTACAGGC AGCTGCACAG GAGCTGAAAG CTGATTATCA CATCAGTGTC 
CCACAGTGCA AGCCCCTGTC CCCTGGGGAG ATCCTAGGCT GCACATCCCC TCGGCTATCC 
AAGGAAGTGG AAGCTGTTGT GTATCTTGGA GATGGCCGCT TTCATCTGGA GTCTGTCATG 
ATTGCCAACC CTAATATACC TGCTTACCGG TATGACCCAT ATGGCAAAGT CCTATCCAGA 
GAATACTATG ACCATCAGCG CATGCAGGCC ACTCGCCAGG AAGCCATTGC TGCTGCACGC 
TCAGCCAAAT CCTGGGGCCT TATTCTGGGA ACCTTGGGCC GCCAGGGCAG TCCCAAGATC 
CTGGAGCACT TGGAATCTCA GCTCAGAAAC TTGGGACTTC CTTTCGTGAG GCTGTTGCTC 
TCTGAGATCT TCCCCAGCAA GCTCAGTCTA CTTCCTGAGG TGGATGTGTG GGTGCAGGTG 
GCATGTCCAC GCCTCTCCAT TGACTGGGGT TCAGCCTTTC CCAAGCCACT GCTGACACCG 
TACGAGGCAG CTGTGGCCCT GAAGGACATT TCTTGGCAGC AACCCTACCC CATGGACTTC 
TACTCTGGCA GCTCCTTAGG GCCATGGACA GTGAACTACG GTCGGGACCG AGCACCTCGG 
GGTCTCTGCC AGCCTGCATC TGACAAGGTG CAGCAAGGGT CCAGAGGCGG CTCTCCAGCC 
CCAGCCTGTG AGAGTTGCAA CTGCGCAGAC CAGAAGGCTA CTTCGCCGGC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of OVCA1/DPH1 in craniofacial abnormalities of Miller-Dieker syndrome.
Human molecular genetics23(21)5579-96(2014 Nov)
Yu YR,You LR,Yan YT,Chen CM

Diphthamide modification on eukaryotic elongation factor 2 is needed to assure fidelity of mRNA translation and mouse development.
Proceedings of the National Academy of Sciences of the United States of America109(34)13817-22(2012 Aug)
Liu S,Bachran C,Gupta P,Miller-Randolph S,Wang H,Crown D,Zhang Y,Wein AN,Singh R,Fattah R,Leppla SH

Ovca1, a candidate gene of the genetic modifier of Tp53, Mop2, affects mouse embryonic lethality.
Genes, chromosomes & cancer47(4)315-25(2008 Apr)
Liang M,Ayanga B,Du S,Godwin AK,Hartsock JK,Evans SC

Gene trap mutagenesis-based forward genetic approach reveals that the tumor suppressor OVCA1 is a component of the biosynthetic pathway of diphthamide on elongation factor 2.
The Journal of biological chemistry280(11)10572-7(2005 Mar)
Nobukuni Y,Kohno K,Miyagawa K

OVCA1: tumor suppressor gene.
Current opinion in genetics & development15(1)49-54(2005 Feb)
Chen CM,Behringer RR