ORF » Species Summary » Arabidopsis thaliana » TWN2 cDNA ORF clone

TWN2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following TWN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TWN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OAb23559 NM_101328.4
Latest version!		 Arabidopsis thaliana valyl-tRNA synthetase / valine-tRNA ligase (VALRS) (TWN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TWN2 ( NM_101328.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_172913.1 Arabidopsis thaliana valyl-tRNA synthetase / valine-tRNA ligase (VALRS) (TWN2), mRNA.
    Price & Availability↑
    CloneID OAb23559
    Clone ID Related Accession (Same CDS sequence) NM_101328.4
    Accession Version NM_101328.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product valyl-tRNA synthetase / valine-tRNA ligase (VALRS)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_101328.3. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACTAC TCTTCCTAAG AAGAGCCAAG CCGTTATTTG TATCTTGTTG TTCAGCTACT 
    CATTCTCGCT CCAGCTTCTT ATCTCCGACG CTTACGAATC AGCTAGTTCG TTCGTTTCAC 
    GGTTCCAGAA CAATGTCGGA ATCAGAGAAG AAGATCCTGA CAGAGGAGGA GCTTGAGCGG 
    AAAAAGAAGA AGGAAGAGAA GGCTAAAGAA AAGGAGTTGA AGAAGCAAAA AGCTTTGGAG 
    AAAGAGAGGC TGGCTGAACT GAAGGCAAAA CAGGCCAAAG ATGGAACTAA TGTCCCTAAA 
    AAGAGCGCAA AGAAGAGTTC CAAACGGGAT GCGTCAGAAG AGAATCCTGA GGATTTTGTG 
    GACCCTGAAA CTCCTCTTGG AGAGAGGAAA AGGCTTTCTT CACAGATGGC CAAACAATAC 
    AGCCCTGCTA CCGTGGAGAA ATCATGGTAT GCTTGGTGGG AGAAATCTGA CCTTTTTAAG 
    GCGGATGCTA AGAGTTCCAA GCCACCTTTT GTAATTGTTC TTCCTCCTCC AAATGTGACT 
    GGAGCCTTGC ATATTGGCCA TGCGCTTACA TCTGCAATTG AGGATACAAT TATTCGTTGG 
    AAGAGAATGT CTGGGTATAA TGCTTTGTGG GTACCCGGGG TTGACCATGC TGGTATAGCT 
    ACACAGGTGG TGGTCGAGAA AAAGATCATG CGTGATAGAG GCATGACTAG GCATGATGTT 
    GGCCGTGAAG AATTCGTAAA GGAAGTGTGG AAGTGGAAGA ACCAGTACGG CGGTACAATT 
    CTAACACAGC TACGGCGACT AGGAGCTTCT CTCGACTGGT CTCGTGAGTG TTTTACAATG 
    GACGAGCAAA GATCAAAAGC TGTCACTGAG GCCTTTGTAC GGTTATACAA AGAAGGGCTT 
    ATATATAGGG ATATTCGGCT TGTTAATTGG GATTGTATTT TGAGAACAGC CATATCTGAT 
    GTTGAGGTTG AGTATATCGA CATTAAAGAA AAAACGCTAC TAAAAGTTCC TGGCTATGAG 
    AAGCCAGTAG AATTTGGTCT TCTTACCTCG TTTGCTTACC CTCTGGAGGG AGGCTTGGGA 
    GAGGTAATTG TTGCCACTAC CAGGGTGGAA ACGATGCTTG GGGATACTGC TATTGCTATT 
    CATCCTGATG ATGCAAGATA CAAGCATCTT CATGGGAAAT TTGCTGTGCA CCCATTTAAT 
    GGGCGGAAGT TACCTATTAT CTGTGATGGA ATACTTGTTG ATCCAAATTT TGGTACTGGT 
    TGTGTTAAGA TTACTCCTGC CCATGATCCC AATGACTGTG AGGTTGGAAA GCGCCACAAG 
    CTGGAATTTA TAAATATATT CACAGATGAT GGAAAAATCA ACACAAATGG TGGGTCTGAC 
    TTCGCAGGGA TGCCACGCTT TGCAGCTCGT GAGGCAGTTG TTGAAGCTCT GCAGAAGCAG 
    GGGCTGTACA GAGGTGCCAA AAACAATGAA ATGAGGCTTG GACTTTGCTC AAGAACTAAT 
    GATGTTATTG AGCCTATGAT AAAGCCTCAA TGGTACGTTA ACTGCAGCAT GATTGGAAAG 
    GAGGCTCTTG ATGTTGCTAT CACTGATGAA AACAAGAAAC TCGAGTTTGT ACCAAAGCAG 
    TACACAGCAG AATGGAGAAG ATGGCTAGAG AACATACGTG ACTGGTGTAT TTCAAGACAG 
    CTTTGGTGGG GACACAGAAT ACCAGCATGG TATGCCACTC TAGAGGAGGA TCAGCTCAAG 
    GAGGTTGGTG CATACAGTGA CCATTGGGTT GTTGCTAGAA CTGAGGACGA TGCTCGAGAA 
    GAGGCTGCTC AGAAATTTTT GGGGAAGAAG TTTGAACTCA CTCGAGATCC TGATGTACTT 
    GATACATGGT TTTCTTCTGG GCTCTTTCCA TTATCTGTTC TGGGATGGCC AGATGTAACC 
    GATGACTTCA AAGCCTTCTA TCCAACTTCT GTTCTTGAAA CTGGACATGA TATCCTCTTT 
    TTTTGGGTTG CTCGTATGGT TATGATGGGA ATGAAACTAG GTGGTGAGGT TCCATTCAGT 
    AAGGTTTATT TCCATCCTAT GATACGTGAT GCACATGGGC GTAAAATGTC AAAATCTCTT 
    GGAAATGTCA TTGATCCACT TGAAGTAATA AACGGAGTAA CTCTTGAAGG TCTTCATAAG 
    AGATTAGAAG AGGGTAACTT GGATCCAAAA GAGGTGATTG TTGCCAAAGA AGGACAAGTG 
    AAGGACTTTC CAAATGGTAT CCCTGAATGT GGCACTGACG CTCTTCGGTT TGCGCTCGTC 
    TCTTACACTG CTCAGTCTGA TAAAATTAAC TTGGACATTC TTCGAGTTGT TGGTTACCGT 
    CAATGGTGTA ATAAACTGTG GAACGCCGTC AGATTTGCAA TGATGAAGCT TGGCGATGGT 
    TATACTCCTC CGCAGACTCT AAGTCCTGAA ACCATGCCTT TCAGCTGCCA ATGGATTCTC 
    TCTGTTCTAA ATAAGGCAAT ATCAAAGACA GTGGTGTCAC TAGATGCATT TGAGTTCTCG 
    GATGCAGCCA ACACAATATA TGCATGGTGG CAATACCAAT TCTGTGATGT GTACATTGAA 
    GCAATTAAGC CTTATTTTGC TGGTGACAAC CCAACGTTTG CTTCAGAGAG GGCTCATGCA 
    CAACATGCTC TCTGGATAAG TCTGGAGACT GGTTTGAGAC TGCTTCACCC ATTTATGCCT 
    TTTGTTACTG AAGAGCTATG GCAACGACTT CCCGCACCCA AAGACACAGA AAGAAAGGCT 
    TCTATCATGA TATGTGACTA CCCATCTGCC ATAGAGAACT GGTCAAATGA GAAAGTGGAG 
    AGCGAAATGG ACACCGTTCT GGCAACTGTG AAATGCATGA GAGCACTCAG GGCAGGGTTG 
    CTGGAGAAAC AGAAAAACGA AAGGTTACCT GCTTTTGCTC TGTGTGAAAA CAACGTAACA 
    TCTGAAATTG TTAAGTCTCA CGAGTTGGAG ATCAGAACCC TTGCAAATCT ATCATCCTTA 
    GAGGTTGTGT CAAAAGGACA ACATGCAGCA CCACCTGGAT CTTCAGTTGA GACGGTGAAC 
    GAGAACCTCA AAGTATATCT TGAAGTGGAC GGAGCCATTA ACACAGAAGC TGAACAAGAA 
    AAAATCAGAA ACAAGATTGG TGAACTACAA AAACAAAAAG AGAAGTTGCA GAAGATGATG 
    AGTGTGTCTA CTTACGAAGA GAAGGTCCCT GCGAATATAA AAGAAGACAA CGCAAACAAG 
    CTTGCTAAGA TACTCCAAGA ATTCGATTTC TTTGAGAAGG AAAGTGCTCG TCTTGCTGCA 
    GAAACAAGCA ATTCAGGAAA TCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_172913.1
    CDS48..3374
    Translation

    Target ORF information:

    RefSeq Version NM_101328.4
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana valyl-tRNA synthetase / valine-tRNA ligase (VALRS) (TWN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_101328.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTCACTAC TCTTCCTAAG AAGAGCCAAG CCGTTATTTG TATCTTGTTG TTCAGCTACT 
    CATTCTCGCT CCAGCTTCTT ATCTCCGACG CTTACGAATC AGCTAGTTCG TTCGTTTCAC 
    GGTTCCAGAA CAATGTCGGA ATCAGAGAAG AAGATCCTGA CAGAGGAGGA GCTTGAGCGG 
    AAAAAGAAGA AGGAAGAGAA GGCTAAAGAA AAGGAGTTGA AGAAGCAAAA AGCTTTGGAG 
    AAAGAGAGGC TGGCTGAACT GAAGGCAAAA CAGGCCAAAG ATGGAACTAA TGTCCCTAAA 
    AAGAGCGCAA AGAAGAGTTC CAAACGGGAT GCGTCAGAAG AGAATCCTGA GGATTTTGTG 
    GACCCTGAAA CTCCTCTTGG AGAGAGGAAA AGGCTTTCTT CACAGATGGC CAAACAATAC 
    AGCCCTGCTA CCGTGGAGAA ATCATGGTAT GCTTGGTGGG AGAAATCTGA CCTTTTTAAG 
    GCGGATGCTA AGAGTTCCAA GCCACCTTTT GTAATTGTTC TTCCTCCTCC AAATGTGACT 
    GGAGCCTTGC ATATTGGCCA TGCGCTTACA TCTGCAATTG AGGATACAAT TATTCGTTGG 
    AAGAGAATGT CTGGGTATAA TGCTTTGTGG GTACCCGGGG TTGACCATGC TGGTATAGCT 
    ACACAGGTGG TGGTCGAGAA AAAGATCATG CGTGATAGAG GCATGACTAG GCATGATGTT 
    GGCCGTGAAG AATTCGTAAA GGAAGTGTGG AAGTGGAAGA ACCAGTACGG CGGTACAATT 
    CTAACACAGC TACGGCGACT AGGAGCTTCT CTCGACTGGT CTCGTGAGTG TTTTACAATG 
    GACGAGCAAA GATCAAAAGC TGTCACTGAG GCCTTTGTAC GGTTATACAA AGAAGGGCTT 
    ATATATAGGG ATATTCGGCT TGTTAATTGG GATTGTATTT TGAGAACAGC CATATCTGAT 
    GTTGAGGTTG AGTATATCGA CATTAAAGAA AAAACGCTAC TAAAAGTTCC TGGCTATGAG 
    AAGCCAGTAG AATTTGGTCT TCTTACCTCG TTTGCTTACC CTCTGGAGGG AGGCTTGGGA 
    GAGGTAATTG TTGCCACTAC CAGGGTGGAA ACGATGCTTG GGGATACTGC TATTGCTATT 
    CATCCTGATG ATGCAAGATA CAAGCATCTT CATGGGAAAT TTGCTGTGCA CCCATTTAAT 
    GGGCGGAAGT TACCTATTAT CTGTGATGGA ATACTTGTTG ATCCAAATTT TGGTACTGGT 
    TGTGTTAAGA TTACTCCTGC CCATGATCCC AATGACTGTG AGGTTGGAAA GCGCCACAAG 
    CTGGAATTTA TAAATATATT CACAGATGAT GGAAAAATCA ACACAAATGG TGGGTCTGAC 
    TTCGCAGGGA TGCCACGCTT TGCAGCTCGT GAGGCAGTTG TTGAAGCTCT GCAGAAGCAG 
    GGGCTGTACA GAGGTGCCAA AAACAATGAA ATGAGGCTTG GACTTTGCTC AAGAACTAAT 
    GATGTTATTG AGCCTATGAT AAAGCCTCAA TGGTACGTTA ACTGCAGCAT GATTGGAAAG 
    GAGGCTCTTG ATGTTGCTAT CACTGATGAA AACAAGAAAC TCGAGTTTGT ACCAAAGCAG 
    TACACAGCAG AATGGAGAAG ATGGCTAGAG AACATACGTG ACTGGTGTAT TTCAAGACAG 
    CTTTGGTGGG GACACAGAAT ACCAGCATGG TATGCCACTC TAGAGGAGGA TCAGCTCAAG 
    GAGGTTGGTG CATACAGTGA CCATTGGGTT GTTGCTAGAA CTGAGGACGA TGCTCGAGAA 
    GAGGCTGCTC AGAAATTTTT GGGGAAGAAG TTTGAACTCA CTCGAGATCC TGATGTACTT 
    GATACATGGT TTTCTTCTGG GCTCTTTCCA TTATCTGTTC TGGGATGGCC AGATGTAACC 
    GATGACTTCA AAGCCTTCTA TCCAACTTCT GTTCTTGAAA CTGGACATGA TATCCTCTTT 
    TTTTGGGTTG CTCGTATGGT TATGATGGGA ATGAAACTAG GTGGTGAGGT TCCATTCAGT 
    AAGGTTTATT TCCATCCTAT GATACGTGAT GCACATGGGC GTAAAATGTC AAAATCTCTT 
    GGAAATGTCA TTGATCCACT TGAAGTAATA AACGGAGTAA CTCTTGAAGG TCTTCATAAG 
    AGATTAGAAG AGGGTAACTT GGATCCAAAA GAGGTGATTG TTGCCAAAGA AGGACAAGTG 
    AAGGACTTTC CAAATGGTAT CCCTGAATGT GGCACTGACG CTCTTCGGTT TGCGCTCGTC 
    TCTTACACTG CTCAGTCTGA TAAAATTAAC TTGGACATTC TTCGAGTTGT TGGTTACCGT 
    CAATGGTGTA ATAAACTGTG GAACGCCGTC AGATTTGCAA TGATGAAGCT TGGCGATGGT 
    TATACTCCTC CGCAGACTCT AAGTCCTGAA ACCATGCCTT TCAGCTGCCA ATGGATTCTC 
    TCTGTTCTAA ATAAGGCAAT ATCAAAGACA GTGGTGTCAC TAGATGCATT TGAGTTCTCG 
    GATGCAGCCA ACACAATATA TGCATGGTGG CAATACCAAT TCTGTGATGT GTACATTGAA 
    GCAATTAAGC CTTATTTTGC TGGTGACAAC CCAACGTTTG CTTCAGAGAG GGCTCATGCA 
    CAACATGCTC TCTGGATAAG TCTGGAGACT GGTTTGAGAC TGCTTCACCC ATTTATGCCT 
    TTTGTTACTG AAGAGCTATG GCAACGACTT CCCGCACCCA AAGACACAGA AAGAAAGGCT 
    TCTATCATGA TATGTGACTA CCCATCTGCC ATAGAGAACT GGTCAAATGA GAAAGTGGAG 
    AGCGAAATGG ACACCGTTCT GGCAACTGTG AAATGCATGA GAGCACTCAG GGCAGGGTTG 
    CTGGAGAAAC AGAAAAACGA AAGGTTACCT GCTTTTGCTC TGTGTGAAAA CAACGTAACA 
    TCTGAAATTG TTAAGTCTCA CGAGTTGGAG ATCAGAACCC TTGCAAATCT ATCATCCTTA 
    GAGGTTGTGT CAAAAGGACA ACATGCAGCA CCACCTGGAT CTTCAGTTGA GACGGTGAAC 
    GAGAACCTCA AAGTATATCT TGAAGTGGAC GGAGCCATTA ACACAGAAGC TGAACAAGAA 
    AAAATCAGAA ACAAGATTGG TGAACTACAA AAACAAAAAG AGAAGTTGCA GAAGATGATG 
    AGTGTGTCTA CTTACGAAGA GAAGGTCCCT GCGAATATAA AAGAAGACAA CGCAAACAAG 
    CTTGCTAAGA TACTCCAAGA ATTCGATTTC TTTGAGAAGG AAAGTGCTCG TCTTGCTGCA 
    GAAACAAGCA ATTCAGGAAA TCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW


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