vars1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vars1
Entrez Gene ID	114427
Full Name	valyl-tRNA synthetase
Synonyms	CHUNP6879,im:7137378,vars1,vars2,wu:fb07a11,wu:fb07d10
General protein information	Preferred Names valyl-tRNA synthetase Names valine--tRNA ligase valyl-tRNA synthetase 1 valyl-tRNA synthetase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001311346.1	NP_001298275.1	valine--tRNA ligase

Pathways from BioSystems

Homologies

	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Neurospora crassa	NCU01965	XP_964329.2
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases.
Waldron AL, Cahan SH, Franklyn CS, Ebert AM
PloS one12(9)e0185317(2017)

p53-Dependent DNA damage response sensitive to editing-defective tRNA synthetase in zebrafish.
Song Y, Shi Y, Carland TM, Lian S, Sasaki T, Schork NJ, Head SR, Kishi S, Schimmel P
Proceedings of the National Academy of Sciences of the United States of America113(30)8460-5(2016 07)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa21179	NM_001311346.1 Latest version!	Danio rerio valyl-tRNA synthetase 1 (vars1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vars1 ( NM_001311346.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001298275.1 Danio rerio valyl-tRNA synthetase 1 (vars1), mRNA.

Price & Availability↑

CloneID	ODa21179
Clone ID Related Accession (Same CDS sequence)	NM_001311346.1
Accession Version	NM_001311346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-11
Organism	Danio rerio(zebrafish)
Product	valine--tRNA ligase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU929328.5. On Aug 8, 2015 this sequence version replaced XM_677715.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01010348.1, GFIL01042936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGACACTC TCTACATCTC TTCCCACCCT GATGACTTCA GGAGTTTGTT GCCCTTGATC 
GCTGCTGGAT TCTGTCCGTC TCCTCCACGA ATTCTCCACC AGGACCCTCC GGCAGAGTTG 
GCCAGATCTC GGCCTGCGCT GGTTTTAGCA GGTGCGGGGG GCACTGTGCT CAGCGGCACC 
AGTGCTGTGA GTTGGTATCT GGCAGCAACA GGCAAACGAG CCGGAAGTGA TAAAAAGCAG 
GAGAGTCAGG TGTGGCAGTG GCTGAGCTTT GCTGAGAATG AGCTGACGCC GGTGGCCTGC 
GCTGTGGCCT TCCCTCTGCT TGGAATCATG GGAGTTGATA AGAAGCTTCA GCAGAGTTCC 
AGAGCGGAGC TGCTGCGTGT TCTGAAAGCT CTGGATGGGA CTCTTGCGCT TCGGACATTC 
TTGGTGGGAG AGAGTGTTAC TCTGGCTGAT GCAGCTGTTG CGATGGCGGC TTTGCTGCCA 
TTCAAATATG CACTGGAGCC TGCAGACAGG AAGTCGTTGG TGAATGTGAC CAGGTGGTTT 
AATACCTGTG TGAATCAGCC ACAGTTTCTG AAGGTATTGG GTAAAATCTC ACTATGTGAG 
AAAATGGTGC CTGTGACCCC CAAACCCAAC ACTGCTTCCA ATGTCACAGT AACTGCTGCT 
GCTGCTGCTA AACCAGACAG CGGACCTGCT AATGGTCCTC CTAAGACAGA AGCTCAACTG 
AAGAAAGAGG CCAAGAAGCG AGAAAAGATG GAGAAGTTTC AACAGAAGAA AGACATGGAG 
GAGAAGAAGA AAATGCAGCC TCAGACTGAG AAAAAGGCCA AACCAGAGAA GAAGGAACTG 
GGTGTGATCA CCTACAATGT CTCCACACCA CCTGGAGAGA AAAAAGATGT CCTGAGTCCT 
CTGCCTGATT CATACAGTCC TCAGTATGTG GAAGCGGCCT GGTACTCCTG GTGGGAGAAA 
CAAGGATTTT TCAAGCCTGA ATATGGGAGG AAGAAACTGA GTGAGCCGAA TCCGCGTGGA 
GTGTTTATGA TGTGTATCCC ACCACCCAAT GTGACTGGAT CCCTTCACCT TGGTCATGCC 
TTGACCAATG CTATTCAGGA CTGCTTGACG AGATGGCACA GGATGCGAGG AGAGACAACG 
CTGTGGAATC CCGGCTGTGA TCACGCTGGC ATCGCCACAC AGGTGGTGGT GGAGAAGAAA 
CTCATGAGAG AGCGGAAGAT GACCAGACAC GACCTTGGGC GAGAGAACTT CATCAAGGAG 
GTCTGGAAGT GGAAGAACGA AAAGGGAGAT CGCATCTATC ACCAGCTGAA GAAGCTGGGC 
TCATCTCTGG ACTGGGACAG AGCTTGTTTT ACCATGGATG ATAAACTGTC ATTTGCGGTT 
CAGGAGGCGT TTATACGCAT GCATGAGGAG GGAGTGATCT ACAGGAGCAA GAGACTCGTC 
AACTGGTCCT GCACACTCAA CTCTGCCATC TCTGACATTG AGGTCGATAA AAAGGAGCTG 
ACGGGGCGAA CTCTTCTTCC CGTGCCGGGT TATAAAGACA AGGTCGTGTT TGGAGTGCTG 
GTGTCATTTT CATACAAGAT CGAAGGATCA GATGAGGAGG TGATAGTGGC CACCACTCGT 
ATTGAGACCA TGCTTGGAGA CACAGCAGTG GCCGTTCACC CGGAAGACTC CAGATATAAG 
CATCTCAAAG GGAAGATGGT CATTCACCCA TTCTGTGACA GAAAGATGCC CGTTGTGTTC 
GATGACTTTG TGGATATGAA CTTTGGAACA GGTGCTGTGA AGATAACTCC TGCTCACGAT 
CATAATGATT ATGAGGTGGG AGAAAGACAC AAGCTGCCCT TCATCAACAT CCTGGATGAA 
AACGGCCTCC TCATCAACGT TCCTCCTCCA TTCCTGGGGA TGAAGCGCTT TGAGGCGAGA 
ACAGCTGTGC TTCAGGCTCT GAAAGACAGA GGCCAGTTTA AGGAGGTCAA AGACAATCCC 
ATGGTGGTGC CTGTCTGCAG CCGTTCTAAA GACATCGTAG AGCCTCTGCT GAAGCCGCAG 
TGGTACGTGG ACTGCAGAGA GATGGGAAAA CAGGCTGCAG ACGTGGTCCG AAATGGAGAG 
CTCAAGATCA TCCCAGACCA CCACCTCAAA ACCTGGTTCA ACTGGATGGA CAACATCAGG 
GACTGGTGCA TCTCCCGGCA GCTGTGGTGG GGTCACAGGA TACCAGCGTA TTTTGTGACT 
GTGAACGACC CCGCGGTTAA ACCAGGAGAG GATGTGGATG GACATTATTG GGTGAGCGGG 
AGATCAGAGC AAGAAGCCAG AGAAAAAGCT GCCAAACGCT TCAATGTCAC TGCAGACAAA 
ATCTCTCTGC GCCAGGATGA GGACGTGTTG GACACATGGT TCTCCTCGGG GATCTTCCCC 
TTCTCCATCT TTGGCTGGCC CAATGAGTCT GAGGATCTGA GAGTCTTCTA CCCGGGCACA 
CTGTTAGAGA CGGGTCATGA TATCTTGTTC TTCTGGGTTG CTCGAATGGT CATGATGGGC 
CTCAAACTCA CTGGAAAACT ACCTTTCAAA GAGGTGTACC TGCATGCAGT GGTGCGAGAT 
GCTCATGGCA GGAAAATGAG CAAGTCTCTT GGTAACGTCA TCGACCCTCT GGACGTCATC 
ACCGGCATAT CACTGGAGGG TCTTTATGCT CAGCTTGCAG ACAGTAATCT GGATCCTCTG 
GAGATTGAGA AAGCCAAACA GGGACAGAAA TCCGACTATC CCACTGGAAT TCCAGAATGT 
GGGACTGATG CGCTTAGATT TGCTCTGTGT GCATACACCA GTCAAGGTAG AGACATTAAT 
CTGGATGTTA ACAGGATTCT GGGATACAGG CATTTCTGTA ACAAGCTGTG GAACGCTGTG 
AAGTTCGCCA TGAGGACTCT GGGAGAGGGG TTTGTGCCTT GTGAGAAAGC TCAGCTGTGT 
GGAAGTGAAA GTGTCTCTGA TCGCTGGATT CTGTCTCGTC TGAGCGCAGC AGTAGCGTTG 
TGTGATGGCG GATTTAAAGC CTACGACTTT CCCACAATCA CCACTGCCAT CTACAATTTC 
TGGCTGTATG AGCTCTGTGA CGTCTATTTG GAGTGTGTGA AGCCGGTGTT TAGCAGGACA 
GACTCTAAGG GTCAGAAACA GGCTGACGTC TGCAGACAGA CCCTCTACAC CTGTCTGGAG 
GTGGGACTGC GCCTCCTGTC CCCCATCATG CCTTTTGTTT CTGAAGAGCT CTTCCAGCGT 
TTGCCAAGGA GAAGGCCTCG GGACAGCCCT CCCAGCATCT CTGTCACACC CTATCCTGAC 
ACTGCTGAGT TCTGCTGGCA CAGTGAGGAT GTGGACCGTC AGATGGAGTT TGTCATGTCT 
GTGGTCAGAA CCATTCGCTC ACTCAGGGCT GATTACAACC TGACTAAGAC TCGTGCTGAC 
TGTTTCCTGC AGTGTATCGA CTCCGAAACG GCCGCTCTGG TTCAGAAATA CAGTCTTCAG 
ATCCAGACCC TGTCTTACTC TCAGGCCGTT CACTCTGTGG TCGGAGACGC CGCTATCCCA 
CAAGGCTGTG CGGTGGCCAT CGCTTCAGAT AAATGCACTG TCAACCTCAT GCTGAAGGGT 
TTGATTGACT TGGGAAAGGA GGTCACGAAA CTGACGGCCA AGAAAGGCGA GCTGGAGAAA 
CAGATGGAGA AGATGAAGGA AAAGATGACA AAGAACGACT ACAAGGAGAA AGTTCCCGTG 
AAAGTGCAGG AAGCTGATGC TGAGAAGCTG AGGCAGAGTG AAGCAGAGCT GCAGAAGGTG 
AAGGAAGCCA TAGAGAACTT CAGCAAGATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298275.1
CDS	84..3899
Translation	MDTLYISSHPDDFRSLLPLIAAGFCPSPPRILHQDPPAELARSRPALVLAGAGGTVLSGTSAVSWYLAATGKRAGSDKKQESQVWQWLSFAENELTPVACAVAFPLLGIMGVDKKLQQSSRAELLRVLKALDGTLALRTFLVGESVTLADAAVAMAALLPFKYALEPADRKSLVNVTRWFNTCVNQPQFLKVLGKISLCEKMVPVTPKPNTASNVTVTAAAAAKPDSGPANGPPKTEAQLKKEAKKREKMEKFQQKKDMEEKKKMQPQTEKKAKPEKKELGVITYNVSTPPGEKKDVLSPLPDSYSPQYVEAAWYSWWEKQGFFKPEYGRKKLSEPNPRGVFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRERKMTRHDLGRENFIKEVWKWKNEKGDRIYHQLKKLGSSLDWDRACFTMDDKLSFAVQEAFIRMHEEGVIYRSKRLVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKDKVVFGVLVSFSYKIEGSDEEVIVATTRIETMLGDTAVAVHPEDSRYKHLKGKMVIHPFCDRKMPVVFDDFVDMNFGTGAVKITPAHDHNDYEVGERHKLPFINILDENGLLINVPPPFLGMKRFEARTAVLQALKDRGQFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVDCREMGKQAADVVRNGELKIIPDHHLKTWFNWMDNIRDWCISRQLWWGHRIPAYFVTVNDPAVKPGEDVDGHYWVSGRSEQEAREKAAKRFNVTADKISLRQDEDVLDTWFSSGIFPFSIFGWPNESEDLRVFYPGTLLETGHDILFFWVARMVMMGLKLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVITGISLEGLYAQLADSNLDPLEIEKAKQGQKSDYPTGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKLWNAVKFAMRTLGEGFVPCEKAQLCGSESVSDRWILSRLSAAVALCDGGFKAYDFPTITTAIYNFWLYELCDVYLECVKPVFSRTDSKGQKQADVCRQTLYTCLEVGLRLLSPIMPFVSEELFQRLPRRRPRDSPPSISVTPYPDTAEFCWHSEDVDRQMEFVMSVVRTIRSLRADYNLTKTRADCFLQCIDSETAALVQKYSLQIQTLSYSQAVHSVVGDAAIPQGCAVAIASDKCTVNLMLKGLIDLGKEVTKLTAKKGELEKQMEKMKEKMTKNDYKEKVPVKVQEADAEKLRQSEAELQKVKEAIENFSKMM

Target ORF information:

RefSeq Version	NM_001311346.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio valyl-tRNA synthetase 1 (vars1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311346.1

ORF Insert Sequence:

ATGGACACTC TCTACATCTC TTCCCACCCT GATGACTTCA GGAGTTTGTT GCCCTTGATC 
GCTGCTGGAT TCTGTCCGTC TCCTCCACGA ATTCTCCACC AGGACCCTCC GGCAGAGTTG 
GCCAGATCTC GGCCTGCGCT GGTTTTAGCA GGTGCGGGGG GCACTGTGCT CAGCGGCACC 
AGTGCTGTGA GTTGGTATCT GGCAGCAACA GGCAAACGAG CCGGAAGTGA TAAAAAGCAG 
GAGAGTCAGG TGTGGCAGTG GCTGAGCTTT GCTGAGAATG AGCTGACGCC GGTGGCCTGC 
GCTGTGGCCT TCCCTCTGCT TGGAATCATG GGAGTTGATA AGAAGCTTCA GCAGAGTTCC 
AGAGCGGAGC TGCTGCGTGT TCTGAAAGCT CTGGATGGGA CTCTTGCGCT TCGGACATTC 
TTGGTGGGAG AGAGTGTTAC TCTGGCTGAT GCAGCTGTTG CGATGGCGGC TTTGCTGCCA 
TTCAAATATG CACTGGAGCC TGCAGACAGG AAGTCGTTGG TGAATGTGAC CAGGTGGTTT 
AATACCTGTG TGAATCAGCC ACAGTTTCTG AAGGTATTGG GTAAAATCTC ACTATGTGAG 
AAAATGGTGC CTGTGACCCC CAAACCCAAC ACTGCTTCCA ATGTCACAGT AACTGCTGCT 
GCTGCTGCTA AACCAGACAG CGGACCTGCT AATGGTCCTC CTAAGACAGA AGCTCAACTG 
AAGAAAGAGG CCAAGAAGCG AGAAAAGATG GAGAAGTTTC AACAGAAGAA AGACATGGAG 
GAGAAGAAGA AAATGCAGCC TCAGACTGAG AAAAAGGCCA AACCAGAGAA GAAGGAACTG 
GGTGTGATCA CCTACAATGT CTCCACACCA CCTGGAGAGA AAAAAGATGT CCTGAGTCCT 
CTGCCTGATT CATACAGTCC TCAGTATGTG GAAGCGGCCT GGTACTCCTG GTGGGAGAAA 
CAAGGATTTT TCAAGCCTGA ATATGGGAGG AAGAAACTGA GTGAGCCGAA TCCGCGTGGA 
GTGTTTATGA TGTGTATCCC ACCACCCAAT GTGACTGGAT CCCTTCACCT TGGTCATGCC 
TTGACCAATG CTATTCAGGA CTGCTTGACG AGATGGCACA GGATGCGAGG AGAGACAACG 
CTGTGGAATC CCGGCTGTGA TCACGCTGGC ATCGCCACAC AGGTGGTGGT GGAGAAGAAA 
CTCATGAGAG AGCGGAAGAT GACCAGACAC GACCTTGGGC GAGAGAACTT CATCAAGGAG 
GTCTGGAAGT GGAAGAACGA AAAGGGAGAT CGCATCTATC ACCAGCTGAA GAAGCTGGGC 
TCATCTCTGG ACTGGGACAG AGCTTGTTTT ACCATGGATG ATAAACTGTC ATTTGCGGTT 
CAGGAGGCGT TTATACGCAT GCATGAGGAG GGAGTGATCT ACAGGAGCAA GAGACTCGTC 
AACTGGTCCT GCACACTCAA CTCTGCCATC TCTGACATTG AGGTCGATAA AAAGGAGCTG 
ACGGGGCGAA CTCTTCTTCC CGTGCCGGGT TATAAAGACA AGGTCGTGTT TGGAGTGCTG 
GTGTCATTTT CATACAAGAT CGAAGGATCA GATGAGGAGG TGATAGTGGC CACCACTCGT 
ATTGAGACCA TGCTTGGAGA CACAGCAGTG GCCGTTCACC CGGAAGACTC CAGATATAAG 
CATCTCAAAG GGAAGATGGT CATTCACCCA TTCTGTGACA GAAAGATGCC CGTTGTGTTC 
GATGACTTTG TGGATATGAA CTTTGGAACA GGTGCTGTGA AGATAACTCC TGCTCACGAT 
CATAATGATT ATGAGGTGGG AGAAAGACAC AAGCTGCCCT TCATCAACAT CCTGGATGAA 
AACGGCCTCC TCATCAACGT TCCTCCTCCA TTCCTGGGGA TGAAGCGCTT TGAGGCGAGA 
ACAGCTGTGC TTCAGGCTCT GAAAGACAGA GGCCAGTTTA AGGAGGTCAA AGACAATCCC 
ATGGTGGTGC CTGTCTGCAG CCGTTCTAAA GACATCGTAG AGCCTCTGCT GAAGCCGCAG 
TGGTACGTGG ACTGCAGAGA GATGGGAAAA CAGGCTGCAG ACGTGGTCCG AAATGGAGAG 
CTCAAGATCA TCCCAGACCA CCACCTCAAA ACCTGGTTCA ACTGGATGGA CAACATCAGG 
GACTGGTGCA TCTCCCGGCA GCTGTGGTGG GGTCACAGGA TACCAGCGTA TTTTGTGACT 
GTGAACGACC CCGCGGTTAA ACCAGGAGAG GATGTGGATG GACATTATTG GGTGAGCGGG 
AGATCAGAGC AAGAAGCCAG AGAAAAAGCT GCCAAACGCT TCAATGTCAC TGCAGACAAA 
ATCTCTCTGC GCCAGGATGA GGACGTGTTG GACACATGGT TCTCCTCGGG GATCTTCCCC 
TTCTCCATCT TTGGCTGGCC CAATGAGTCT GAGGATCTGA GAGTCTTCTA CCCGGGCACA 
CTGTTAGAGA CGGGTCATGA TATCTTGTTC TTCTGGGTTG CTCGAATGGT CATGATGGGC 
CTCAAACTCA CTGGAAAACT ACCTTTCAAA GAGGTGTACC TGCATGCAGT GGTGCGAGAT 
GCTCATGGCA GGAAAATGAG CAAGTCTCTT GGTAACGTCA TCGACCCTCT GGACGTCATC 
ACCGGCATAT CACTGGAGGG TCTTTATGCT CAGCTTGCAG ACAGTAATCT GGATCCTCTG 
GAGATTGAGA AAGCCAAACA GGGACAGAAA TCCGACTATC CCACTGGAAT TCCAGAATGT 
GGGACTGATG CGCTTAGATT TGCTCTGTGT GCATACACCA GTCAAGGTAG AGACATTAAT 
CTGGATGTTA ACAGGATTCT GGGATACAGG CATTTCTGTA ACAAGCTGTG GAACGCTGTG 
AAGTTCGCCA TGAGGACTCT GGGAGAGGGG TTTGTGCCTT GTGAGAAAGC TCAGCTGTGT 
GGAAGTGAAA GTGTCTCTGA TCGCTGGATT CTGTCTCGTC TGAGCGCAGC AGTAGCGTTG 
TGTGATGGCG GATTTAAAGC CTACGACTTT CCCACAATCA CCACTGCCAT CTACAATTTC 
TGGCTGTATG AGCTCTGTGA CGTCTATTTG GAGTGTGTGA AGCCGGTGTT TAGCAGGACA 
GACTCTAAGG GTCAGAAACA GGCTGACGTC TGCAGACAGA CCCTCTACAC CTGTCTGGAG 
GTGGGACTGC GCCTCCTGTC CCCCATCATG CCTTTTGTTT CTGAAGAGCT CTTCCAGCGT 
TTGCCAAGGA GAAGGCCTCG GGACAGCCCT CCCAGCATCT CTGTCACACC CTATCCTGAC 
ACTGCTGAGT TCTGCTGGCA CAGTGAGGAT GTGGACCGTC AGATGGAGTT TGTCATGTCT 
GTGGTCAGAA CCATTCGCTC ACTCAGGGCT GATTACAACC TGACTAAGAC TCGTGCTGAC 
TGTTTCCTGC AGTGTATCGA CTCCGAAACG GCCGCTCTGG TTCAGAAATA CAGTCTTCAG 
ATCCAGACCC TGTCTTACTC TCAGGCCGTT CACTCTGTGG TCGGAGACGC CGCTATCCCA 
CAAGGCTGTG CGGTGGCCAT CGCTTCAGAT AAATGCACTG TCAACCTCAT GCTGAAGGGT 
TTGATTGACT TGGGAAAGGA GGTCACGAAA CTGACGGCCA AGAAAGGCGA GCTGGAGAAA 
CAGATGGAGA AGATGAAGGA AAAGATGACA AAGAACGACT ACAAGGAGAA AGTTCCCGTG 
AAAGTGCAGG AAGCTGATGC TGAGAAGCTG AGGCAGAGTG AAGCAGAGCT GCAGAAGGTG 
AAGGAAGCCA TAGAGAACTT CAGCAAGATG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases.
PloS one12(9)e0185317(2017)
Waldron AL,Cahan SH,Franklyn CS,Ebert AM

p53-Dependent DNA damage response sensitive to editing-defective tRNA synthetase in zebrafish.
Proceedings of the National Academy of Sciences of the United States of America113(30)8460-5(2016 07)
Song Y,Shi Y,Carland TM,Lian S,Sasaki T,Schork NJ,Head SR,Kishi S,Schimmel P

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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vars1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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