VARS cDNA ORF clone, Canis lupus familiaris(Dog)

The following VARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe15046 XM_005627162.2
Latest version!
Canis lupus familiaris valyl-tRNA synthetase (VARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe15047 XM_538837.7
Latest version!
Canis lupus familiaris valyl-tRNA synthetase (VARS), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OCe15047 XM_538837.6 Canis lupus familiaris valyl-tRNA synthetase (VARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe15046
    Clone ID Related Accession (Same CDS sequence) XM_005627162.2
    Accession Version XM_005627162.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCGTCC TGTACGTCTC CCCTCATCCC GACGCCTTCC CCAGCCTCCG GGCCCTCATA 
    GCCGCGCGCT ACGGGGAGGC CGGGGAGGGC CCGGGCTGGG GAGGAGCCCA CCCGCGCATC
    TGCCTCCAGC CGCCCCCGAC CAGCAGGACC CCCTGCCCCC CACCGCGTCT GCCCGCCCTG
    GAGCGGGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GTTGCTGTGG
    CCGGCGGGCC TGGGGGGCCC CGGGGGGAGC CGGGCCGCCG TGCTGGTCCA ACAGTGGGTC
    AGCTACGCCG ACACGGAGCT GATACCGGCC GCCTGCGGAG CAACGCTGCC CGCCTTGGGA
    CTCCGGAGCG CTGCCCAGGA CCCCCAGGCT GCCCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACCTGGCTG GGGAGGCCCC CACACTGGCT
    GACCTGGCAG CTGTCACGGC CTTGCTCCTG CCTTTCCGTT ACGTCCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA GCCAGAATTC
    CGGGCAGTGC TGGGAGAGGT GGTTCTGTAC TCAGGGGCCA GACTCCTCTC TCAACAGCCA
    GGCCCCGAGG TCCCTGCACC CCCAAAGACG GCCTCTCAAC TCAAAAAGGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAGCAGAAG CAGAAGACAC AACAGCAGCA GCCACCGCCG
    GCGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGGAAAAC GGGATCCTGG GGTCATTACA
    TATGACCTCC CAACCCCAGC TGGGGAAAAG AAAGATGTCA GTGGCACCAT GCCCGACTCC
    TACAGCCCTC AGTATGTGGA AGCTGCCTGG TACCCATGGT GGGAGCAGCA GGGCTTCTTC
    CAGCCCGAGT ATGGACGTTC CAGCGTGTCA GCGGCAAATC CCCGGGGAGT CTTCATGATG
    TGCATCCCAC CCCCCAATGT GACAGGCTCC CTGCACCTGG GCCACGCACT CACCAATGCC
    ATCCAGGACT CCCTGACCCG ATGGCATCGC ATGCGCGGGG AGACCACCCT GTGGAACCCT
    GGCTGTGACC ACGCAGGCAT TGCCACCCAG GTGGTGGTGG AGAAGAAACT GTGGCGTGAG
    CAGGGCCTGA GCCGGCACCA GCTGGGCCGA GAGGCCTTCC TCCGGGAGGT CTGGAAATGG
    AAAGAGGAGA AAGGGGACCG GATTTATCAC CAGCTGAAGA AGCTTGGGAG CTCCTTGGAC
    TGGAATCGAG CCTGTTTCAC CATGGACCCT AAACTCTCAG CAGCTGTGAC AGAGGCCTTT
    GTCCGGCTCC ATGAGGAGGG AGTCATCTAC CGCAGTACCC GCCTTGTCAA TTGGTCCTGC
    ACCCTCAATT CTGCCATCTC CGACATCGAG GTGGATAAGA AGGAGCTGAC CGGTCGCACT
    CTGCTCTCCG TGCCTGGCTA TAAGGAGAAG GTGGAGTTTG GGGTCCTTGT CTCCTTTGCC
    TATAAGATCC AAGGCTCAGA CGGTGACGAG GAGGTGGTGG TGGCAACAAC TCGGATTGAA
    ACAATGCTGG GAGACGTGGC CGTAGCTGTG CACCCCAAAG ATCCTAGATA CCAGCACCTG
    AAGGGGAGGA GTGTGATCCA CCCATTCTTG TCTCGGAGCT TGCCGATCAT CTTTGACGAT
    TTTGTGGACA TGGAGTTTGG CACAGGTGCG GTGAAGATCA CCCCTGCCCA TGACCAGAAC
    GACTACGAGG TCGGACGGCG GCACAGGCTG GAGGCCATCA CCATCATGGA CTCCCAAGGG
    GCCCTCATCA ATGTGCCCCC GCCTTTCCTG GGCCTACCCA GGTTTGAGGC CAGGAAGGCG
    GTGCTGGCAG CGCTGAAGGA GCAGGGGCTG TTCCGGGGTG TTGAGGACAA CCCCATGGTA
    GTGCCGCTTT GCAACCGGTC CAAGGATGTG GTAGAGCCTC TGCTGCGGCC GCAGTGGTAC
    GTGCGCTGTG GGGAGATGGC CCAGGCCGCC AGTGCCGCTG TGACGCGGGG AGACCTCCGC
    ATCCTGCCCG AGGCCCATCA GCGGACTTGG CACACCTGGA TGGACAACAT CCGGGACTGG
    TGCATCTCCC GGCAGCTGTG GTGGGGTCAC CGCATCCCAG CCTACTTTGT CACAGTCAGC
    GACCCGGCTG TGCCCCCGGG GGAGGACCCC GACGAGCGGT ACTGGGTGAG CGGGCGCAGC
    AAGGCTGAGG CCCGGGAGAA GGCAGCCAAG GAGTTTGGAG TGTCGTCTGA CAAGATCAGT
    CTCCAGCAAG ATGAGGACGT ACTGGACACC TGGTTCTCCT CTGGCCTCTT CCCCTTCTCC
    ATCTTTGGCT GGCCCAACCA ATCAGAAGAT CTGAGTGTGT TCTACCCGGG GACGCTGCTG
    GAGACGGGCC ATGACATCCT TTTCTTCTGG GTGGCCAGGA TGGTCATGCT TGGCCTGAAG
    CTCACCGGCA AGCTGCCCTT CAGAGAGGTC TACCTTCATG CCATCGTGCG AGATGCCCAC
    GGCCGAAAGA TGAGCAAGTC TTTAGGCAAT GTTATCGACC CCCTGGACGT CATCCATGGA
    GTCTCCCTGC AGGGCCTCCA TGACCAGTTA CTGAACAGCA ACCTGGACCC CAGTGAGGTG
    GAGAAGGCTA AAGATGGCCA GAAGGCAGAC TTTCCGGCAG GCATTCCCGA GTGTGGCACC
    GACGCCCTCC GGTTTGGACT CTGTGCCTAC ACGTCCCAGG GTCGTGACAT TAACCTGGAT
    GTGAACCGGA TATTGGGGTA CCGCCACTTC TGCAACAAGC TCTGGAACGC CACCAAGTTT
    GCCCTCCGGG GCCTGGGGAA GGGTTTTGTG CCCTCAGCCA CCTCTGAGCC TGGAGGCCAC
    GAGAGCCTGG TGGACCGCTG GATCCGCAGC CGGCTGACTG AGGCGGTGAG GCTTAGCAAC
    CAAGGCTTCC AGGCCTACGA TTTCCCAGCT GTCACCACTG CTCAGTACAG CTTCTGGCTC
    TATGAGCTCT GTGATGTCTA CCTGGAGTGC CTGAAGCCCG TGCTGAATGG GATGGACCAC
    GTGGTAGCCG AGTGTGCTCG CCAGACCCTC TACACCTGCC TGGATGTTGG CCTGCGGCTG
    CTGTCACCCT TCATGCCCTT TGTGACAGAG GAGCTGTTCC AGAGGCTGCC CCGGCGGACA
    CTACAAGCAC CCCCTAGTCT CTGTGTTACC CCCTATCCGG AGCCCTCAGA GTGCTCCTGG
    AAGGACCCTG AGGCGGAAGC TGCCATGGAA CTGGCCCTAA GTATCACACG AGCCGTGCGC
    TCCCTGCGTG CGGACTACAA CCTGACCCGG ATCAGGCCTG ACTGTTTCCT GGAAGTGGCT
    GATGAAGCCA CAGGCATTCT GGCATCGGCA GTGTCGGCCT ACGTGCAGGC GCTGGCCAGC
    GCGGGTGTGG TGGCTGTCCT GGCCCTGGGG GCTCCTGCAC CACAGGGCTG TGCTGTGGCC
    CTGGCATCTG ACCGTTGCTC CGTCCACCTG CAGCTGCAGG GCCTGGTGGA CCCGGCCCGG
    GAACTGGGCA AGCTGCAGGC CAAGCGCAGC GAGGCACAGC GGCAGGCACA GCGTCTGCGG
    GAGCGCCGCG CTGCCTCAGG CTACCCTGTC AAGGTGCCCC TTGAAGTCCA GGAGGCCGAC
    AGAGCCAAGC TCCAACAGAT AGAAGCAGAG CTCAAGAAGG TGGATGAGGC CATTGCGCTA
    TTCCAGAAGA TGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627219.1
    CDS260..4057
    Translation

    Target ORF information:

    RefSeq Version XM_005627162.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase (VARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627162.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCGTCC TGTACGTCTC CCCTCATCCC GACGCCTTCC CCAGCCTCCG GGCCCTCATA 
    GCCGCGCGCT ACGGGGAGGC CGGGGAGGGC CCGGGCTGGG GAGGAGCCCA CCCGCGCATC
    TGCCTCCAGC CGCCCCCGAC CAGCAGGACC CCCTGCCCCC CACCGCGTCT GCCCGCCCTG
    GAGCGGGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GTTGCTGTGG
    CCGGCGGGCC TGGGGGGCCC CGGGGGGAGC CGGGCCGCCG TGCTGGTCCA ACAGTGGGTC
    AGCTACGCCG ACACGGAGCT GATACCGGCC GCCTGCGGAG CAACGCTGCC CGCCTTGGGA
    CTCCGGAGCG CTGCCCAGGA CCCCCAGGCT GCCCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACCTGGCTG GGGAGGCCCC CACACTGGCT
    GACCTGGCAG CTGTCACGGC CTTGCTCCTG CCTTTCCGTT ACGTCCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA GCCAGAATTC
    CGGGCAGTGC TGGGAGAGGT GGTTCTGTAC TCAGGGGCCA GACTCCTCTC TCAACAGCCA
    GGCCCCGAGG TCCCTGCACC CCCAAAGACG GCCTCTCAAC TCAAAAAGGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAGCAGAAG CAGAAGACAC AACAGCAGCA GCCACCGCCG
    GCGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGGAAAAC GGGATCCTGG GGTCATTACA
    TATGACCTCC CAACCCCAGC TGGGGAAAAG AAAGATGTCA GTGGCACCAT GCCCGACTCC
    TACAGCCCTC AGTATGTGGA AGCTGCCTGG TACCCATGGT GGGAGCAGCA GGGCTTCTTC
    CAGCCCGAGT ATGGACGTTC CAGCGTGTCA GCGGCAAATC CCCGGGGAGT CTTCATGATG
    TGCATCCCAC CCCCCAATGT GACAGGCTCC CTGCACCTGG GCCACGCACT CACCAATGCC
    ATCCAGGACT CCCTGACCCG ATGGCATCGC ATGCGCGGGG AGACCACCCT GTGGAACCCT
    GGCTGTGACC ACGCAGGCAT TGCCACCCAG GTGGTGGTGG AGAAGAAACT GTGGCGTGAG
    CAGGGCCTGA GCCGGCACCA GCTGGGCCGA GAGGCCTTCC TCCGGGAGGT CTGGAAATGG
    AAAGAGGAGA AAGGGGACCG GATTTATCAC CAGCTGAAGA AGCTTGGGAG CTCCTTGGAC
    TGGAATCGAG CCTGTTTCAC CATGGACCCT AAACTCTCAG CAGCTGTGAC AGAGGCCTTT
    GTCCGGCTCC ATGAGGAGGG AGTCATCTAC CGCAGTACCC GCCTTGTCAA TTGGTCCTGC
    ACCCTCAATT CTGCCATCTC CGACATCGAG GTGGATAAGA AGGAGCTGAC CGGTCGCACT
    CTGCTCTCCG TGCCTGGCTA TAAGGAGAAG GTGGAGTTTG GGGTCCTTGT CTCCTTTGCC
    TATAAGATCC AAGGCTCAGA CGGTGACGAG GAGGTGGTGG TGGCAACAAC TCGGATTGAA
    ACAATGCTGG GAGACGTGGC CGTAGCTGTG CACCCCAAAG ATCCTAGATA CCAGCACCTG
    AAGGGGAGGA GTGTGATCCA CCCATTCTTG TCTCGGAGCT TGCCGATCAT CTTTGACGAT
    TTTGTGGACA TGGAGTTTGG CACAGGTGCG GTGAAGATCA CCCCTGCCCA TGACCAGAAC
    GACTACGAGG TCGGACGGCG GCACAGGCTG GAGGCCATCA CCATCATGGA CTCCCAAGGG
    GCCCTCATCA ATGTGCCCCC GCCTTTCCTG GGCCTACCCA GGTTTGAGGC CAGGAAGGCG
    GTGCTGGCAG CGCTGAAGGA GCAGGGGCTG TTCCGGGGTG TTGAGGACAA CCCCATGGTA
    GTGCCGCTTT GCAACCGGTC CAAGGATGTG GTAGAGCCTC TGCTGCGGCC GCAGTGGTAC
    GTGCGCTGTG GGGAGATGGC CCAGGCCGCC AGTGCCGCTG TGACGCGGGG AGACCTCCGC
    ATCCTGCCCG AGGCCCATCA GCGGACTTGG CACACCTGGA TGGACAACAT CCGGGACTGG
    TGCATCTCCC GGCAGCTGTG GTGGGGTCAC CGCATCCCAG CCTACTTTGT CACAGTCAGC
    GACCCGGCTG TGCCCCCGGG GGAGGACCCC GACGAGCGGT ACTGGGTGAG CGGGCGCAGC
    AAGGCTGAGG CCCGGGAGAA GGCAGCCAAG GAGTTTGGAG TGTCGTCTGA CAAGATCAGT
    CTCCAGCAAG ATGAGGACGT ACTGGACACC TGGTTCTCCT CTGGCCTCTT CCCCTTCTCC
    ATCTTTGGCT GGCCCAACCA ATCAGAAGAT CTGAGTGTGT TCTACCCGGG GACGCTGCTG
    GAGACGGGCC ATGACATCCT TTTCTTCTGG GTGGCCAGGA TGGTCATGCT TGGCCTGAAG
    CTCACCGGCA AGCTGCCCTT CAGAGAGGTC TACCTTCATG CCATCGTGCG AGATGCCCAC
    GGCCGAAAGA TGAGCAAGTC TTTAGGCAAT GTTATCGACC CCCTGGACGT CATCCATGGA
    GTCTCCCTGC AGGGCCTCCA TGACCAGTTA CTGAACAGCA ACCTGGACCC CAGTGAGGTG
    GAGAAGGCTA AAGATGGCCA GAAGGCAGAC TTTCCGGCAG GCATTCCCGA GTGTGGCACC
    GACGCCCTCC GGTTTGGACT CTGTGCCTAC ACGTCCCAGG GTCGTGACAT TAACCTGGAT
    GTGAACCGGA TATTGGGGTA CCGCCACTTC TGCAACAAGC TCTGGAACGC CACCAAGTTT
    GCCCTCCGGG GCCTGGGGAA GGGTTTTGTG CCCTCAGCCA CCTCTGAGCC TGGAGGCCAC
    GAGAGCCTGG TGGACCGCTG GATCCGCAGC CGGCTGACTG AGGCGGTGAG GCTTAGCAAC
    CAAGGCTTCC AGGCCTACGA TTTCCCAGCT GTCACCACTG CTCAGTACAG CTTCTGGCTC
    TATGAGCTCT GTGATGTCTA CCTGGAGTGC CTGAAGCCCG TGCTGAATGG GATGGACCAC
    GTGGTAGCCG AGTGTGCTCG CCAGACCCTC TACACCTGCC TGGATGTTGG CCTGCGGCTG
    CTGTCACCCT TCATGCCCTT TGTGACAGAG GAGCTGTTCC AGAGGCTGCC CCGGCGGACA
    CTACAAGCAC CCCCTAGTCT CTGTGTTACC CCCTATCCGG AGCCCTCAGA GTGCTCCTGG
    AAGGACCCTG AGGCGGAAGC TGCCATGGAA CTGGCCCTAA GTATCACACG AGCCGTGCGC
    TCCCTGCGTG CGGACTACAA CCTGACCCGG ATCAGGCCTG ACTGTTTCCT GGAAGTGGCT
    GATGAAGCCA CAGGCATTCT GGCATCGGCA GTGTCGGCCT ACGTGCAGGC GCTGGCCAGC
    GCGGGTGTGG TGGCTGTCCT GGCCCTGGGG GCTCCTGCAC CACAGGGCTG TGCTGTGGCC
    CTGGCATCTG ACCGTTGCTC CGTCCACCTG CAGCTGCAGG GCCTGGTGGA CCCGGCCCGG
    GAACTGGGCA AGCTGCAGGC CAAGCGCAGC GAGGCACAGC GGCAGGCACA GCGTCTGCGG
    GAGCGCCGCG CTGCCTCAGG CTACCCTGTC AAGGTGCCCC TTGAAGTCCA GGAGGCCGAC
    AGAGCCAAGC TCCAACAGAT AGAAGCAGAG CTCAAGAAGG TGGATGAGGC CATTGCGCTA
    TTCCAGAAGA TGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15047
    Clone ID Related Accession (Same CDS sequence) XM_538837.7 , XM_538837.6
    Accession Version XM_538837.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product valine--tRNA ligase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538837.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCGTCC TGTACGTCTC CCCTCATCCC GACGCCTTCC CCAGCCTCCG GGCCCTCATA 
    GCCGCGCGCT ACGGGGAGGC CGGGGAGGGC CCGGGCTGGG GAGGAGCCCA CCCGCGCATC
    TGCCTCCAGC CGCCCCCGAC CAGCAGGACC CCCTGCCCCC CACCGCGTCT GCCCGCCCTG
    GAGCGGGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GTTGCTGTGG
    CCGGCGGGCC TGGGGGGCCC CGGGGGGAGC CGGGCCGCCG TGCTGGTCCA ACAGTGGGTC
    AGCTACGCCG ACACGGAGCT GATACCGGCC GCCTGCGGAG CAACGCTGCC CGCCTTGGGA
    CTCCGGAGCG CTGCCCAGGA CCCCCAGGCT GCCCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACCTGGCTG GGGAGGCCCC CACACTGGCT
    GACCTGGCAG CTGTCACGGC CTTGCTCCTG CCTTTCCGTT ACGTCCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA GCCAGAATTC
    CGGGCAGTGC TGGGAGAGGT GGTTCTGTAC TCAGGGGCCA GACTCCTCTC TCAACAGCCA
    GGCCCCGAGG TCCCTGCACC CCCAAAGACG GCCTCTCAAC TCAAAAAGGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAGCAGAAG CAGAAGACAC AACAGCAGCA GCCACCGCCG
    GCGGAGAAGA AACCAAAACC AGAGAAGAGG GGAAAACGGG ATCCTGGGGT CATTACATAT
    GACCTCCCAA CCCCAGCTGG GGAAAAGAAA GATGTCAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAAGC TGCCTGGTAC CCATGGTGGG AGCAGCAGGG CTTCTTCCAG
    CCCGAGTATG GACGTTCCAG CGTGTCAGCG GCAAATCCCC GGGGAGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ACGCACTCAC CAATGCCATC
    CAGGACTCCC TGACCCGATG GCATCGCATG CGCGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCACG CAGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTGTG GCGTGAGCAG
    GGCCTGAGCC GGCACCAGCT GGGCCGAGAG GCCTTCCTCC GGGAGGTCTG GAAATGGAAA
    GAGGAGAAAG GGGACCGGAT TTATCACCAG CTGAAGAAGC TTGGGAGCTC CTTGGACTGG
    AATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTCCATG AGGAGGGAGT CATCTACCGC AGTACCCGCC TTGTCAATTG GTCCTGCACC
    CTCAATTCTG CCATCTCCGA CATCGAGGTG GATAAGAAGG AGCTGACCGG TCGCACTCTG
    CTCTCCGTGC CTGGCTATAA GGAGAAGGTG GAGTTTGGGG TCCTTGTCTC CTTTGCCTAT
    AAGATCCAAG GCTCAGACGG TGACGAGGAG GTGGTGGTGG CAACAACTCG GATTGAAACA
    ATGCTGGGAG ACGTGGCCGT AGCTGTGCAC CCCAAAGATC CTAGATACCA GCACCTGAAG
    GGGAGGAGTG TGATCCACCC ATTCTTGTCT CGGAGCTTGC CGATCATCTT TGACGATTTT
    GTGGACATGG AGTTTGGCAC AGGTGCGGTG AAGATCACCC CTGCCCATGA CCAGAACGAC
    TACGAGGTCG GACGGCGGCA CAGGCTGGAG GCCATCACCA TCATGGACTC CCAAGGGGCC
    CTCATCAATG TGCCCCCGCC TTTCCTGGGC CTACCCAGGT TTGAGGCCAG GAAGGCGGTG
    CTGGCAGCGC TGAAGGAGCA GGGGCTGTTC CGGGGTGTTG AGGACAACCC CATGGTAGTG
    CCGCTTTGCA ACCGGTCCAA GGATGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTG
    CGCTGTGGGG AGATGGCCCA GGCCGCCAGT GCCGCTGTGA CGCGGGGAGA CCTCCGCATC
    CTGCCCGAGG CCCATCAGCG GACTTGGCAC ACCTGGATGG ACAACATCCG GGACTGGTGC
    ATCTCCCGGC AGCTGTGGTG GGGTCACCGC ATCCCAGCCT ACTTTGTCAC AGTCAGCGAC
    CCGGCTGTGC CCCCGGGGGA GGACCCCGAC GAGCGGTACT GGGTGAGCGG GCGCAGCAAG
    GCTGAGGCCC GGGAGAAGGC AGCCAAGGAG TTTGGAGTGT CGTCTGACAA GATCAGTCTC
    CAGCAAGATG AGGACGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC
    TTTGGCTGGC CCAACCAATC AGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG
    ACGGGCCATG ACATCCTTTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTGAAGCTC
    ACCGGCAAGC TGCCCTTCAG AGAGGTCTAC CTTCATGCCA TCGTGCGAGA TGCCCACGGC
    CGAAAGATGA GCAAGTCTTT AGGCAATGTT ATCGACCCCC TGGACGTCAT CCATGGAGTC
    TCCCTGCAGG GCCTCCATGA CCAGTTACTG AACAGCAACC TGGACCCCAG TGAGGTGGAG
    AAGGCTAAAG ATGGCCAGAA GGCAGACTTT CCGGCAGGCA TTCCCGAGTG TGGCACCGAC
    GCCCTCCGGT TTGGACTCTG TGCCTACACG TCCCAGGGTC GTGACATTAA CCTGGATGTG
    AACCGGATAT TGGGGTACCG CCACTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC
    CTCCGGGGCC TGGGGAAGGG TTTTGTGCCC TCAGCCACCT CTGAGCCTGG AGGCCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACTGAGG CGGTGAGGCT TAGCAACCAA
    GGCTTCCAGG CCTACGATTT CCCAGCTGTC ACCACTGCTC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACCT GGAGTGCCTG AAGCCCGTGC TGAATGGGAT GGACCACGTG
    GTAGCCGAGT GTGCTCGCCA GACCCTCTAC ACCTGCCTGG ATGTTGGCCT GCGGCTGCTG
    TCACCCTTCA TGCCCTTTGT GACAGAGGAG CTGTTCCAGA GGCTGCCCCG GCGGACACTA
    CAAGCACCCC CTAGTCTCTG TGTTACCCCC TATCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAGG CGGAAGCTGC CATGGAACTG GCCCTAAGTA TCACACGAGC CGTGCGCTCC
    CTGCGTGCGG ACTACAACCT GACCCGGATC AGGCCTGACT GTTTCCTGGA AGTGGCTGAT
    GAAGCCACAG GCATTCTGGC ATCGGCAGTG TCGGCCTACG TGCAGGCGCT GGCCAGCGCG
    GGTGTGGTGG CTGTCCTGGC CCTGGGGGCT CCTGCACCAC AGGGCTGTGC TGTGGCCCTG
    GCATCTGACC GTTGCTCCGT CCACCTGCAG CTGCAGGGCC TGGTGGACCC GGCCCGGGAA
    CTGGGCAAGC TGCAGGCCAA GCGCAGCGAG GCACAGCGGC AGGCACAGCG TCTGCGGGAG
    CGCCGCGCTG CCTCAGGCTA CCCTGTCAAG GTGCCCCTTG AAGTCCAGGA GGCCGACAGA
    GCCAAGCTCC AACAGATAGA AGCAGAGCTC AAGAAGGTGG ATGAGGCCAT TGCGCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538837.3
    CDS194..3988
    Translation

    Target ORF information:

    RefSeq Version XM_538837.7
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase (VARS), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538837.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCGTCC TGTACGTCTC CCCTCATCCC GACGCCTTCC CCAGCCTCCG GGCCCTCATA 
    GCCGCGCGCT ACGGGGAGGC CGGGGAGGGC CCGGGCTGGG GAGGAGCCCA CCCGCGCATC
    TGCCTCCAGC CGCCCCCGAC CAGCAGGACC CCCTGCCCCC CACCGCGTCT GCCCGCCCTG
    GAGCGGGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GTTGCTGTGG
    CCGGCGGGCC TGGGGGGCCC CGGGGGGAGC CGGGCCGCCG TGCTGGTCCA ACAGTGGGTC
    AGCTACGCCG ACACGGAGCT GATACCGGCC GCCTGCGGAG CAACGCTGCC CGCCTTGGGA
    CTCCGGAGCG CTGCCCAGGA CCCCCAGGCT GCCCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACCTGGCTG GGGAGGCCCC CACACTGGCT
    GACCTGGCAG CTGTCACGGC CTTGCTCCTG CCTTTCCGTT ACGTCCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA GCCAGAATTC
    CGGGCAGTGC TGGGAGAGGT GGTTCTGTAC TCAGGGGCCA GACTCCTCTC TCAACAGCCA
    GGCCCCGAGG TCCCTGCACC CCCAAAGACG GCCTCTCAAC TCAAAAAGGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAGCAGAAG CAGAAGACAC AACAGCAGCA GCCACCGCCG
    GCGGAGAAGA AACCAAAACC AGAGAAGAGG GGAAAACGGG ATCCTGGGGT CATTACATAT
    GACCTCCCAA CCCCAGCTGG GGAAAAGAAA GATGTCAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAAGC TGCCTGGTAC CCATGGTGGG AGCAGCAGGG CTTCTTCCAG
    CCCGAGTATG GACGTTCCAG CGTGTCAGCG GCAAATCCCC GGGGAGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ACGCACTCAC CAATGCCATC
    CAGGACTCCC TGACCCGATG GCATCGCATG CGCGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCACG CAGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTGTG GCGTGAGCAG
    GGCCTGAGCC GGCACCAGCT GGGCCGAGAG GCCTTCCTCC GGGAGGTCTG GAAATGGAAA
    GAGGAGAAAG GGGACCGGAT TTATCACCAG CTGAAGAAGC TTGGGAGCTC CTTGGACTGG
    AATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTCCATG AGGAGGGAGT CATCTACCGC AGTACCCGCC TTGTCAATTG GTCCTGCACC
    CTCAATTCTG CCATCTCCGA CATCGAGGTG GATAAGAAGG AGCTGACCGG TCGCACTCTG
    CTCTCCGTGC CTGGCTATAA GGAGAAGGTG GAGTTTGGGG TCCTTGTCTC CTTTGCCTAT
    AAGATCCAAG GCTCAGACGG TGACGAGGAG GTGGTGGTGG CAACAACTCG GATTGAAACA
    ATGCTGGGAG ACGTGGCCGT AGCTGTGCAC CCCAAAGATC CTAGATACCA GCACCTGAAG
    GGGAGGAGTG TGATCCACCC ATTCTTGTCT CGGAGCTTGC CGATCATCTT TGACGATTTT
    GTGGACATGG AGTTTGGCAC AGGTGCGGTG AAGATCACCC CTGCCCATGA CCAGAACGAC
    TACGAGGTCG GACGGCGGCA CAGGCTGGAG GCCATCACCA TCATGGACTC CCAAGGGGCC
    CTCATCAATG TGCCCCCGCC TTTCCTGGGC CTACCCAGGT TTGAGGCCAG GAAGGCGGTG
    CTGGCAGCGC TGAAGGAGCA GGGGCTGTTC CGGGGTGTTG AGGACAACCC CATGGTAGTG
    CCGCTTTGCA ACCGGTCCAA GGATGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTG
    CGCTGTGGGG AGATGGCCCA GGCCGCCAGT GCCGCTGTGA CGCGGGGAGA CCTCCGCATC
    CTGCCCGAGG CCCATCAGCG GACTTGGCAC ACCTGGATGG ACAACATCCG GGACTGGTGC
    ATCTCCCGGC AGCTGTGGTG GGGTCACCGC ATCCCAGCCT ACTTTGTCAC AGTCAGCGAC
    CCGGCTGTGC CCCCGGGGGA GGACCCCGAC GAGCGGTACT GGGTGAGCGG GCGCAGCAAG
    GCTGAGGCCC GGGAGAAGGC AGCCAAGGAG TTTGGAGTGT CGTCTGACAA GATCAGTCTC
    CAGCAAGATG AGGACGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC
    TTTGGCTGGC CCAACCAATC AGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG
    ACGGGCCATG ACATCCTTTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTGAAGCTC
    ACCGGCAAGC TGCCCTTCAG AGAGGTCTAC CTTCATGCCA TCGTGCGAGA TGCCCACGGC
    CGAAAGATGA GCAAGTCTTT AGGCAATGTT ATCGACCCCC TGGACGTCAT CCATGGAGTC
    TCCCTGCAGG GCCTCCATGA CCAGTTACTG AACAGCAACC TGGACCCCAG TGAGGTGGAG
    AAGGCTAAAG ATGGCCAGAA GGCAGACTTT CCGGCAGGCA TTCCCGAGTG TGGCACCGAC
    GCCCTCCGGT TTGGACTCTG TGCCTACACG TCCCAGGGTC GTGACATTAA CCTGGATGTG
    AACCGGATAT TGGGGTACCG CCACTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC
    CTCCGGGGCC TGGGGAAGGG TTTTGTGCCC TCAGCCACCT CTGAGCCTGG AGGCCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACTGAGG CGGTGAGGCT TAGCAACCAA
    GGCTTCCAGG CCTACGATTT CCCAGCTGTC ACCACTGCTC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACCT GGAGTGCCTG AAGCCCGTGC TGAATGGGAT GGACCACGTG
    GTAGCCGAGT GTGCTCGCCA GACCCTCTAC ACCTGCCTGG ATGTTGGCCT GCGGCTGCTG
    TCACCCTTCA TGCCCTTTGT GACAGAGGAG CTGTTCCAGA GGCTGCCCCG GCGGACACTA
    CAAGCACCCC CTAGTCTCTG TGTTACCCCC TATCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAGG CGGAAGCTGC CATGGAACTG GCCCTAAGTA TCACACGAGC CGTGCGCTCC
    CTGCGTGCGG ACTACAACCT GACCCGGATC AGGCCTGACT GTTTCCTGGA AGTGGCTGAT
    GAAGCCACAG GCATTCTGGC ATCGGCAGTG TCGGCCTACG TGCAGGCGCT GGCCAGCGCG
    GGTGTGGTGG CTGTCCTGGC CCTGGGGGCT CCTGCACCAC AGGGCTGTGC TGTGGCCCTG
    GCATCTGACC GTTGCTCCGT CCACCTGCAG CTGCAGGGCC TGGTGGACCC GGCCCGGGAA
    CTGGGCAAGC TGCAGGCCAA GCGCAGCGAG GCACAGCGGC AGGCACAGCG TCTGCGGGAG
    CGCCGCGCTG CCTCAGGCTA CCCTGTCAAG GTGCCCCTTG AAGTCCAGGA GGCCGACAGA
    GCCAAGCTCC AACAGATAGA AGCAGAGCTC AAGAAGGTGG ATGAGGCCAT TGCGCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15047
    Clone ID Related Accession (Same CDS sequence) XM_538837.7 , XM_538837.6
    Accession Version XM_538837.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product valine--tRNA ligase isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538837.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCGTCC TGTACGTCTC CCCTCATCCC GACGCCTTCC CCAGCCTCCG GGCCCTCATA 
    GCCGCGCGCT ACGGGGAGGC CGGGGAGGGC CCGGGCTGGG GAGGAGCCCA CCCGCGCATC
    TGCCTCCAGC CGCCCCCGAC CAGCAGGACC CCCTGCCCCC CACCGCGTCT GCCCGCCCTG
    GAGCGGGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GTTGCTGTGG
    CCGGCGGGCC TGGGGGGCCC CGGGGGGAGC CGGGCCGCCG TGCTGGTCCA ACAGTGGGTC
    AGCTACGCCG ACACGGAGCT GATACCGGCC GCCTGCGGAG CAACGCTGCC CGCCTTGGGA
    CTCCGGAGCG CTGCCCAGGA CCCCCAGGCT GCCCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACCTGGCTG GGGAGGCCCC CACACTGGCT
    GACCTGGCAG CTGTCACGGC CTTGCTCCTG CCTTTCCGTT ACGTCCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA GCCAGAATTC
    CGGGCAGTGC TGGGAGAGGT GGTTCTGTAC TCAGGGGCCA GACTCCTCTC TCAACAGCCA
    GGCCCCGAGG TCCCTGCACC CCCAAAGACG GCCTCTCAAC TCAAAAAGGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAGCAGAAG CAGAAGACAC AACAGCAGCA GCCACCGCCG
    GCGGAGAAGA AACCAAAACC AGAGAAGAGG GGAAAACGGG ATCCTGGGGT CATTACATAT
    GACCTCCCAA CCCCAGCTGG GGAAAAGAAA GATGTCAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAAGC TGCCTGGTAC CCATGGTGGG AGCAGCAGGG CTTCTTCCAG
    CCCGAGTATG GACGTTCCAG CGTGTCAGCG GCAAATCCCC GGGGAGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ACGCACTCAC CAATGCCATC
    CAGGACTCCC TGACCCGATG GCATCGCATG CGCGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCACG CAGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTGTG GCGTGAGCAG
    GGCCTGAGCC GGCACCAGCT GGGCCGAGAG GCCTTCCTCC GGGAGGTCTG GAAATGGAAA
    GAGGAGAAAG GGGACCGGAT TTATCACCAG CTGAAGAAGC TTGGGAGCTC CTTGGACTGG
    AATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTCCATG AGGAGGGAGT CATCTACCGC AGTACCCGCC TTGTCAATTG GTCCTGCACC
    CTCAATTCTG CCATCTCCGA CATCGAGGTG GATAAGAAGG AGCTGACCGG TCGCACTCTG
    CTCTCCGTGC CTGGCTATAA GGAGAAGGTG GAGTTTGGGG TCCTTGTCTC CTTTGCCTAT
    AAGATCCAAG GCTCAGACGG TGACGAGGAG GTGGTGGTGG CAACAACTCG GATTGAAACA
    ATGCTGGGAG ACGTGGCCGT AGCTGTGCAC CCCAAAGATC CTAGATACCA GCACCTGAAG
    GGGAGGAGTG TGATCCACCC ATTCTTGTCT CGGAGCTTGC CGATCATCTT TGACGATTTT
    GTGGACATGG AGTTTGGCAC AGGTGCGGTG AAGATCACCC CTGCCCATGA CCAGAACGAC
    TACGAGGTCG GACGGCGGCA CAGGCTGGAG GCCATCACCA TCATGGACTC CCAAGGGGCC
    CTCATCAATG TGCCCCCGCC TTTCCTGGGC CTACCCAGGT TTGAGGCCAG GAAGGCGGTG
    CTGGCAGCGC TGAAGGAGCA GGGGCTGTTC CGGGGTGTTG AGGACAACCC CATGGTAGTG
    CCGCTTTGCA ACCGGTCCAA GGATGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTG
    CGCTGTGGGG AGATGGCCCA GGCCGCCAGT GCCGCTGTGA CGCGGGGAGA CCTCCGCATC
    CTGCCCGAGG CCCATCAGCG GACTTGGCAC ACCTGGATGG ACAACATCCG GGACTGGTGC
    ATCTCCCGGC AGCTGTGGTG GGGTCACCGC ATCCCAGCCT ACTTTGTCAC AGTCAGCGAC
    CCGGCTGTGC CCCCGGGGGA GGACCCCGAC GAGCGGTACT GGGTGAGCGG GCGCAGCAAG
    GCTGAGGCCC GGGAGAAGGC AGCCAAGGAG TTTGGAGTGT CGTCTGACAA GATCAGTCTC
    CAGCAAGATG AGGACGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC
    TTTGGCTGGC CCAACCAATC AGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG
    ACGGGCCATG ACATCCTTTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTGAAGCTC
    ACCGGCAAGC TGCCCTTCAG AGAGGTCTAC CTTCATGCCA TCGTGCGAGA TGCCCACGGC
    CGAAAGATGA GCAAGTCTTT AGGCAATGTT ATCGACCCCC TGGACGTCAT CCATGGAGTC
    TCCCTGCAGG GCCTCCATGA CCAGTTACTG AACAGCAACC TGGACCCCAG TGAGGTGGAG
    AAGGCTAAAG ATGGCCAGAA GGCAGACTTT CCGGCAGGCA TTCCCGAGTG TGGCACCGAC
    GCCCTCCGGT TTGGACTCTG TGCCTACACG TCCCAGGGTC GTGACATTAA CCTGGATGTG
    AACCGGATAT TGGGGTACCG CCACTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC
    CTCCGGGGCC TGGGGAAGGG TTTTGTGCCC TCAGCCACCT CTGAGCCTGG AGGCCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACTGAGG CGGTGAGGCT TAGCAACCAA
    GGCTTCCAGG CCTACGATTT CCCAGCTGTC ACCACTGCTC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACCT GGAGTGCCTG AAGCCCGTGC TGAATGGGAT GGACCACGTG
    GTAGCCGAGT GTGCTCGCCA GACCCTCTAC ACCTGCCTGG ATGTTGGCCT GCGGCTGCTG
    TCACCCTTCA TGCCCTTTGT GACAGAGGAG CTGTTCCAGA GGCTGCCCCG GCGGACACTA
    CAAGCACCCC CTAGTCTCTG TGTTACCCCC TATCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAGG CGGAAGCTGC CATGGAACTG GCCCTAAGTA TCACACGAGC CGTGCGCTCC
    CTGCGTGCGG ACTACAACCT GACCCGGATC AGGCCTGACT GTTTCCTGGA AGTGGCTGAT
    GAAGCCACAG GCATTCTGGC ATCGGCAGTG TCGGCCTACG TGCAGGCGCT GGCCAGCGCG
    GGTGTGGTGG CTGTCCTGGC CCTGGGGGCT CCTGCACCAC AGGGCTGTGC TGTGGCCCTG
    GCATCTGACC GTTGCTCCGT CCACCTGCAG CTGCAGGGCC TGGTGGACCC GGCCCGGGAA
    CTGGGCAAGC TGCAGGCCAA GCGCAGCGAG GCACAGCGGC AGGCACAGCG TCTGCGGGAG
    CGCCGCGCTG CCTCAGGCTA CCCTGTCAAG GTGCCCCTTG AAGTCCAGGA GGCCGACAGA
    GCCAAGCTCC AACAGATAGA AGCAGAGCTC AAGAAGGTGG ATGAGGCCAT TGCGCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538837.3
    CDS260..4054
    Translation

    Target ORF information:

    RefSeq Version XM_538837.6
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris valyl-tRNA synthetase (VARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538837.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCCGTCC TGTACGTCTC CCCTCATCCC GACGCCTTCC CCAGCCTCCG GGCCCTCATA 
    GCCGCGCGCT ACGGGGAGGC CGGGGAGGGC CCGGGCTGGG GAGGAGCCCA CCCGCGCATC
    TGCCTCCAGC CGCCCCCGAC CAGCAGGACC CCCTGCCCCC CACCGCGTCT GCCCGCCCTG
    GAGCGGGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GTTGCTGTGG
    CCGGCGGGCC TGGGGGGCCC CGGGGGGAGC CGGGCCGCCG TGCTGGTCCA ACAGTGGGTC
    AGCTACGCCG ACACGGAGCT GATACCGGCC GCCTGCGGAG CAACGCTGCC CGCCTTGGGA
    CTCCGGAGCG CTGCCCAGGA CCCCCAGGCT GCCCTGGGGG CCCTGGGCAG GGCCCTGAGC
    CCCTTGGAAG AGTGGCTTCG GCTGCACACC TACCTGGCTG GGGAGGCCCC CACACTGGCT
    GACCTGGCAG CTGTCACGGC CTTGCTCCTG CCTTTCCGTT ACGTCCTGGA CCCTTCTGCC
    CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA GCCAGAATTC
    CGGGCAGTGC TGGGAGAGGT GGTTCTGTAC TCAGGGGCCA GACTCCTCTC TCAACAGCCA
    GGCCCCGAGG TCCCTGCACC CCCAAAGACG GCCTCTCAAC TCAAAAAGGA GGCAAAGAAA
    CGGGAGAAGC TAGAGAAATT CCAGCAGAAG CAGAAGACAC AACAGCAGCA GCCACCGCCG
    GCGGAGAAGA AACCAAAACC AGAGAAGAGG GGAAAACGGG ATCCTGGGGT CATTACATAT
    GACCTCCCAA CCCCAGCTGG GGAAAAGAAA GATGTCAGTG GCACCATGCC CGACTCCTAC
    AGCCCTCAGT ATGTGGAAGC TGCCTGGTAC CCATGGTGGG AGCAGCAGGG CTTCTTCCAG
    CCCGAGTATG GACGTTCCAG CGTGTCAGCG GCAAATCCCC GGGGAGTCTT CATGATGTGC
    ATCCCACCCC CCAATGTGAC AGGCTCCCTG CACCTGGGCC ACGCACTCAC CAATGCCATC
    CAGGACTCCC TGACCCGATG GCATCGCATG CGCGGGGAGA CCACCCTGTG GAACCCTGGC
    TGTGACCACG CAGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTGTG GCGTGAGCAG
    GGCCTGAGCC GGCACCAGCT GGGCCGAGAG GCCTTCCTCC GGGAGGTCTG GAAATGGAAA
    GAGGAGAAAG GGGACCGGAT TTATCACCAG CTGAAGAAGC TTGGGAGCTC CTTGGACTGG
    AATCGAGCCT GTTTCACCAT GGACCCTAAA CTCTCAGCAG CTGTGACAGA GGCCTTTGTC
    CGGCTCCATG AGGAGGGAGT CATCTACCGC AGTACCCGCC TTGTCAATTG GTCCTGCACC
    CTCAATTCTG CCATCTCCGA CATCGAGGTG GATAAGAAGG AGCTGACCGG TCGCACTCTG
    CTCTCCGTGC CTGGCTATAA GGAGAAGGTG GAGTTTGGGG TCCTTGTCTC CTTTGCCTAT
    AAGATCCAAG GCTCAGACGG TGACGAGGAG GTGGTGGTGG CAACAACTCG GATTGAAACA
    ATGCTGGGAG ACGTGGCCGT AGCTGTGCAC CCCAAAGATC CTAGATACCA GCACCTGAAG
    GGGAGGAGTG TGATCCACCC ATTCTTGTCT CGGAGCTTGC CGATCATCTT TGACGATTTT
    GTGGACATGG AGTTTGGCAC AGGTGCGGTG AAGATCACCC CTGCCCATGA CCAGAACGAC
    TACGAGGTCG GACGGCGGCA CAGGCTGGAG GCCATCACCA TCATGGACTC CCAAGGGGCC
    CTCATCAATG TGCCCCCGCC TTTCCTGGGC CTACCCAGGT TTGAGGCCAG GAAGGCGGTG
    CTGGCAGCGC TGAAGGAGCA GGGGCTGTTC CGGGGTGTTG AGGACAACCC CATGGTAGTG
    CCGCTTTGCA ACCGGTCCAA GGATGTGGTA GAGCCTCTGC TGCGGCCGCA GTGGTACGTG
    CGCTGTGGGG AGATGGCCCA GGCCGCCAGT GCCGCTGTGA CGCGGGGAGA CCTCCGCATC
    CTGCCCGAGG CCCATCAGCG GACTTGGCAC ACCTGGATGG ACAACATCCG GGACTGGTGC
    ATCTCCCGGC AGCTGTGGTG GGGTCACCGC ATCCCAGCCT ACTTTGTCAC AGTCAGCGAC
    CCGGCTGTGC CCCCGGGGGA GGACCCCGAC GAGCGGTACT GGGTGAGCGG GCGCAGCAAG
    GCTGAGGCCC GGGAGAAGGC AGCCAAGGAG TTTGGAGTGT CGTCTGACAA GATCAGTCTC
    CAGCAAGATG AGGACGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC
    TTTGGCTGGC CCAACCAATC AGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG
    ACGGGCCATG ACATCCTTTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTGAAGCTC
    ACCGGCAAGC TGCCCTTCAG AGAGGTCTAC CTTCATGCCA TCGTGCGAGA TGCCCACGGC
    CGAAAGATGA GCAAGTCTTT AGGCAATGTT ATCGACCCCC TGGACGTCAT CCATGGAGTC
    TCCCTGCAGG GCCTCCATGA CCAGTTACTG AACAGCAACC TGGACCCCAG TGAGGTGGAG
    AAGGCTAAAG ATGGCCAGAA GGCAGACTTT CCGGCAGGCA TTCCCGAGTG TGGCACCGAC
    GCCCTCCGGT TTGGACTCTG TGCCTACACG TCCCAGGGTC GTGACATTAA CCTGGATGTG
    AACCGGATAT TGGGGTACCG CCACTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC
    CTCCGGGGCC TGGGGAAGGG TTTTGTGCCC TCAGCCACCT CTGAGCCTGG AGGCCACGAG
    AGCCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACTGAGG CGGTGAGGCT TAGCAACCAA
    GGCTTCCAGG CCTACGATTT CCCAGCTGTC ACCACTGCTC AGTACAGCTT CTGGCTCTAT
    GAGCTCTGTG ATGTCTACCT GGAGTGCCTG AAGCCCGTGC TGAATGGGAT GGACCACGTG
    GTAGCCGAGT GTGCTCGCCA GACCCTCTAC ACCTGCCTGG ATGTTGGCCT GCGGCTGCTG
    TCACCCTTCA TGCCCTTTGT GACAGAGGAG CTGTTCCAGA GGCTGCCCCG GCGGACACTA
    CAAGCACCCC CTAGTCTCTG TGTTACCCCC TATCCGGAGC CCTCAGAGTG CTCCTGGAAG
    GACCCTGAGG CGGAAGCTGC CATGGAACTG GCCCTAAGTA TCACACGAGC CGTGCGCTCC
    CTGCGTGCGG ACTACAACCT GACCCGGATC AGGCCTGACT GTTTCCTGGA AGTGGCTGAT
    GAAGCCACAG GCATTCTGGC ATCGGCAGTG TCGGCCTACG TGCAGGCGCT GGCCAGCGCG
    GGTGTGGTGG CTGTCCTGGC CCTGGGGGCT CCTGCACCAC AGGGCTGTGC TGTGGCCCTG
    GCATCTGACC GTTGCTCCGT CCACCTGCAG CTGCAGGGCC TGGTGGACCC GGCCCGGGAA
    CTGGGCAAGC TGCAGGCCAA GCGCAGCGAG GCACAGCGGC AGGCACAGCG TCTGCGGGAG
    CGCCGCGCTG CCTCAGGCTA CCCTGTCAAG GTGCCCCTTG AAGTCCAGGA GGCCGACAGA
    GCCAAGCTCC AACAGATAGA AGCAGAGCTC AAGAAGGTGG ATGAGGCCAT TGCGCTATTC
    CAGAAGATGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.