ValRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ValRS
Entrez Gene ID	45783
Full Name	Valyl-tRNA synthetase
Synonyms	Aats-val,BEST:LD45671,CG4062,Dmel\CG4062,VRS,aats-val,l(2)03531,l(2)rI255,ld45671
General protein information	Preferred Names Valyl-tRNA synthetase Names CG4062 gene product from transcript CG4062-RC CG4062-PA CG4062-PB CG4062-PC ValRS-PA ValRS-PB ValRS-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F7-49F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_165970.2	NP_725259.1	Valyl-tRNA synthetase, isoform B
NM_080099.3	NP_524838.1	Valyl-tRNA synthetase, isoform A
NM_001274017.1	NP_001260946.1	Valyl-tRNA synthetase, isoform C

Pathways from BioSystems

Homologies

	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Neurospora crassa	NCU01965	XP_964329.2
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ValRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ValRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11418	NM_001274017.1 Latest version!	Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12713	NM_080099.3 Latest version!	Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12713	NM_165970.2 Latest version!	Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ValRS ( NM_001274017.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260946.1 Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11418
Clone ID Related Accession (Same CDS sequence)	NM_001274017.1
Accession Version	NM_001274017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Valyl-tRNA synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAGG CTGAACAGCA GAACGATGCC AACGCACCAG CCGGAGCCGG GGGTGATCCT 
CCCAAGACGG CCAAGCAACT GGAAAAGGAG CGTCTGAAGG CGGAGAAGCT GGCCAAGCTG 
CAGGCGAAGC TGGACAAGAA GGCTGCGGCT GCTCCAGCAG CGGGTGAGAA GAAGGAGAAG 
CCCGAGAAGC GCACCAAGGA GGTGAAAGAG GCTGCCGTGT ACACGGCCCA AACCGCGCCC 
GGTGAGAAGA AGGATCTGAG TGGAGCCTTG CCGGATGCCT ACAGTCCACG GTATGTGGAA 
GCACAGTGGT ACAGCTGGTG GGAGAAGGAG GGCTTCTTCA CGCCCGAGTA CGGACGCGCA 
TCCATAGACG CCCCCAATCC CAATGGCAAG TTTGTGATGA TCATTCCACC ACCAAACGTG 
ACGGGCTCTC TGCATTTGGG TCACGCCCTG ACCAACGCCA TCGAGGACGC CATCACGCGC 
TACCACCGCA TGAAGGGACG AACCACTCTA TGGGTGCCTG GTTGCGACCA CGCCGGCATC 
GCCACCCAGG TGGTGGTAGA GAAGCTATTG TGGCGCGACG AGAAGCTATC GCGGCACGAC 
CTAGGTCGCG AAAAGTTCAT TGAGCGCATC TGGGACTGGC GCCGGGAAAA GGGCGGTCGC 
ATCTATGAGC AGCTGAAGTC CCTGGGCTCC TCCTATGACT GGACCCGAGT GGCCTTCACC 
ATGGACCCCA AGCTCTGTCG CGCCGTTACC GAGGCTTTCG TCCGATTGCA CGAGGAGGGC 
AGCATCTACC GTAGCTCCCG ACTGGTCAAT TGGTCGTGCA CCCTACGATC GGCTATTTCG 
GACATTGAAG TGGACAAGGT GGAGATTCCC GGTCGCACAT TCCTCTCCAT TCCCGGATAC 
GAGGACAAAG TGGAATTTGG TGTTCTAATA AAGTTCGCCT ACAAGGTGGA AGGCAGTGAC 
GAAGAGATCA TTGTAGCCAC CACCCGTATC GAGACTATGC TGGGCGATAC TGCTGTGGCT 
GTGCATCCCC AAGACGATCG GTACAAGCAC CTGCATGGCA AGTTCGTAGT CCATCCGTTC 
TCCACGCGCC GCCTGCCAAT CGTCTGTGAC GAGTTCGTGG ACATGGCTTT CGGTACGGGT 
GCTGTGAAGA TCACCCCAGC CCACGATCCC AACGATTACG AGGTGGGCAA GCGCTGCAAT 
TTGCCCTTCA TTACAATCTT CAACGACGAC GGCTACATAA TCGGCGACTA CGGAGAGTTT 
ACTGGAATGA AGCGGTTCGA GTGCCGCAAG AAAATCCTGG AGAAGCTTAA GGCCCTGAAT 
CTATACCGCG AGACATTGAA CAACCCGATG GTGGTGCCCA TCTGCAGTCG CTCCAAGGAC 
GTGGTGGAGC CGCTGATTAA GCCGCAGTGG TATGTTAGTT GCTCGGACAT GGCAGCCTCT 
GCCACTGAGG CAGTGCGCTC CGGAGAACTA AAGATCATTC CGGAGCATCA CACCAAGACG 
TGGTACCACT GGATGGACGG TATTCGCGAC TGGTGCGTGT CGAGGCAGCT GTGGTGGGGT 
CACCGGATTC CGGCATACCA TGTCAGCTTC ACCGACCCTT CTCTTCAAAC TGGATCGTCT 
GCTCATGGAA GTGTGAACGA CGACGAGCAG TACTGGATTG TAGCACGCAG CGAAGCGGAG 
GCTCTGACCA AGGCAGCCGA ACGTTTTGGA GTGGACGCCA GCAAGATAGT ACTGAAACAA 
GACGAAGATG TCCTGGACAC ATGGTTCAGT TCGGGAATCT TTCCCTTCTC CGTTTTTGGC 
TGGCCAGACC AAACTAAGGA TCTGCAAACG TTCTATCCCA CATCGCTGTT GGAGACGGGT 
CACGACATTC TCTTCTTCTG GGTGGCTCGC ATGGTATTCT TTGGACAGAA GCTGCTGGGC 
AAACTGCCGT TTAAGGAGGT CTATCTTCAC CCCATGGTGA GGGATGCGCA CGGACGCAAA 
ATGTCCAAGT CCCTGGGAAA TGTTATCGAT CCTATGGATG TTATTCGTGG CATTACGCTC 
GAGGGACTGC ATGCTCAACT TGTGGGCTCC AACCTGGATC CTCGGGAGAT CGAAAAGGCT 
AAGGCTGGAC AGAAACAGGA TTATCCGCAG GGAATTCCCG AGTGCGGCTC GGATGCACTT 
CGATTCGCGC TGTGCGCCTA TATCACGCAA GCGCGCGACA TCAACCTGGA CATCAATCGC 
GTTCTGGGAT ATCGTTTCTT CTGCAACAAG CTGTGGAACG CCACCAAGTT CGCCCTGCTG 
TACTTCACCG GCTCTGAGAA GTTTGACACG GAGCTAAGTG CGTCTGCAGC GATCAACCAG 
ATGGACGCCT GGATCCTGTC ACGCTTGGCA GCTGCCATAG AAGCTTGCAA CACCGGCTTT 
GAGTCGTACG ACTTTGCTGC AGCGACCAGC GCCTGCTACG CATTCTGGCT CTACGATCTG 
TGCGACGTGT ATTTGGAGTG TCTGAAGCCG ATCTTCCAGA GCGGCAGCGA GGAGCAGCAG 
ACCGCCGCAA GGCGCACGCT CTACGTATGT CTAGATTATG GACTGCGCTT ACTCTCCCCG 
TTCATGCCTT TCATCACGGA GGAACTTTAC CAGCGGCTTC CGAGGGCAAA TCCCGCTCCT 
AGCATTTGCG TGGCTAGCTA TCCCAGCAAT ACATCCTGGC GCAGCACAAA GATAGAATCG 
GACGTTGAAT TTGTGCAGAA GGCGGCGCGA ATCATCCGAT CTGCCCGTTC CGACTATAAT 
CTGCCCAACA AGGTTAAAAC CGAGGTGTAT ATAGTATGCA CGGATTCGGT GCCCAGTGAA 
ATACTCAAAC GCTACGCCAG CGATCTGGCC ACAATCTCCT ACTGCTCCAA TGTGGTCTTT 
GACAGTCCCG CACCGCAGGG CTGTGCGATC CTCACCGTGA CGGGCCAGTG CGAGGTGCAC 
CTGCTGCTCA AGGGACTCGT GGAGGCGGAC AAGGAGATAG CCAAGCTGCA GAAGAAGAGC 
GACCAGCTGG TGCAGACGGT GGGCAAGCTA ACTCAGGCCA TCCAGGCCGC CGACTACGCA 
ACTAAGGTTC CCGCCGAGGT GCAGGAGGCC AACGAGACAA AACTCTGCGA GTCGCGGGCA 
GAGATCGAAC GCATCGCTGC CGCCATCGAG ACTCTGAAAC TAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260946.1
CDS	109..3276
Translation	MPEAEQQNDANAPAGAGGDPPKTAKQLEKERLKAEKLAKLQAKLDKKAAAAPAAGEKKEKPEKRTKEVKEAAVYTAQTAPGEKKDLSGALPDAYSPRYVEAQWYSWWEKEGFFTPEYGRASIDAPNPNGKFVMIIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLLWRDEKLSRHDLGREKFIERIWDWRREKGGRIYEQLKSLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLSIPGYEDKVEFGVLIKFAYKVEGSDEEIIVATTRIETMLGDTAVAVHPQDDRYKHLHGKFVVHPFSTRRLPIVCDEFVDMAFGTGAVKITPAHDPNDYEVGKRCNLPFITIFNDDGYIIGDYGEFTGMKRFECRKKILEKLKALNLYRETLNNPMVVPICSRSKDVVEPLIKPQWYVSCSDMAASATEAVRSGELKIIPEHHTKTWYHWMDGIRDWCVSRQLWWGHRIPAYHVSFTDPSLQTGSSAHGSVNDDEQYWIVARSEAEALTKAAERFGVDASKIVLKQDEDVLDTWFSSGIFPFSVFGWPDQTKDLQTFYPTSLLETGHDILFFWVARMVFFGQKLLGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPMDVIRGITLEGLHAQLVGSNLDPREIEKAKAGQKQDYPQGIPECGSDALRFALCAYITQARDINLDINRVLGYRFFCNKLWNATKFALLYFTGSEKFDTELSASAAINQMDAWILSRLAAAIEACNTGFESYDFAAATSACYAFWLYDLCDVYLECLKPIFQSGSEEQQTAARRTLYVCLDYGLRLLSPFMPFITEELYQRLPRANPAPSICVASYPSNTSWRSTKIESDVEFVQKAARIIRSARSDYNLPNKVKTEVYIVCTDSVPSEILKRYASDLATISYCSNVVFDSPAPQGCAILTVTGQCEVHLLLKGLVEADKEIAKLQKKSDQLVQTVGKLTQAIQAADYATKVPAEVQEANETKLCESRAEIERIAAAIETLKLM

Target ORF information:

RefSeq Version	NM_001274017.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274017.1

ORF Insert Sequence:

ATGCCCGAGG CTGAACAGCA GAACGATGCC AACGCACCAG CCGGAGCCGG GGGTGATCCT 
CCCAAGACGG CCAAGCAACT GGAAAAGGAG CGTCTGAAGG CGGAGAAGCT GGCCAAGCTG 
CAGGCGAAGC TGGACAAGAA GGCTGCGGCT GCTCCAGCAG CGGGTGAGAA GAAGGAGAAG 
CCCGAGAAGC GCACCAAGGA GGTGAAAGAG GCTGCCGTGT ACACGGCCCA AACCGCGCCC 
GGTGAGAAGA AGGATCTGAG TGGAGCCTTG CCGGATGCCT ACAGTCCACG GTATGTGGAA 
GCACAGTGGT ACAGCTGGTG GGAGAAGGAG GGCTTCTTCA CGCCCGAGTA CGGACGCGCA 
TCCATAGACG CCCCCAATCC CAATGGCAAG TTTGTGATGA TCATTCCACC ACCAAACGTG 
ACGGGCTCTC TGCATTTGGG TCACGCCCTG ACCAACGCCA TCGAGGACGC CATCACGCGC 
TACCACCGCA TGAAGGGACG AACCACTCTA TGGGTGCCTG GTTGCGACCA CGCCGGCATC 
GCCACCCAGG TGGTGGTAGA GAAGCTATTG TGGCGCGACG AGAAGCTATC GCGGCACGAC 
CTAGGTCGCG AAAAGTTCAT TGAGCGCATC TGGGACTGGC GCCGGGAAAA GGGCGGTCGC 
ATCTATGAGC AGCTGAAGTC CCTGGGCTCC TCCTATGACT GGACCCGAGT GGCCTTCACC 
ATGGACCCCA AGCTCTGTCG CGCCGTTACC GAGGCTTTCG TCCGATTGCA CGAGGAGGGC 
AGCATCTACC GTAGCTCCCG ACTGGTCAAT TGGTCGTGCA CCCTACGATC GGCTATTTCG 
GACATTGAAG TGGACAAGGT GGAGATTCCC GGTCGCACAT TCCTCTCCAT TCCCGGATAC 
GAGGACAAAG TGGAATTTGG TGTTCTAATA AAGTTCGCCT ACAAGGTGGA AGGCAGTGAC 
GAAGAGATCA TTGTAGCCAC CACCCGTATC GAGACTATGC TGGGCGATAC TGCTGTGGCT 
GTGCATCCCC AAGACGATCG GTACAAGCAC CTGCATGGCA AGTTCGTAGT CCATCCGTTC 
TCCACGCGCC GCCTGCCAAT CGTCTGTGAC GAGTTCGTGG ACATGGCTTT CGGTACGGGT 
GCTGTGAAGA TCACCCCAGC CCACGATCCC AACGATTACG AGGTGGGCAA GCGCTGCAAT 
TTGCCCTTCA TTACAATCTT CAACGACGAC GGCTACATAA TCGGCGACTA CGGAGAGTTT 
ACTGGAATGA AGCGGTTCGA GTGCCGCAAG AAAATCCTGG AGAAGCTTAA GGCCCTGAAT 
CTATACCGCG AGACATTGAA CAACCCGATG GTGGTGCCCA TCTGCAGTCG CTCCAAGGAC 
GTGGTGGAGC CGCTGATTAA GCCGCAGTGG TATGTTAGTT GCTCGGACAT GGCAGCCTCT 
GCCACTGAGG CAGTGCGCTC CGGAGAACTA AAGATCATTC CGGAGCATCA CACCAAGACG 
TGGTACCACT GGATGGACGG TATTCGCGAC TGGTGCGTGT CGAGGCAGCT GTGGTGGGGT 
CACCGGATTC CGGCATACCA TGTCAGCTTC ACCGACCCTT CTCTTCAAAC TGGATCGTCT 
GCTCATGGAA GTGTGAACGA CGACGAGCAG TACTGGATTG TAGCACGCAG CGAAGCGGAG 
GCTCTGACCA AGGCAGCCGA ACGTTTTGGA GTGGACGCCA GCAAGATAGT ACTGAAACAA 
GACGAAGATG TCCTGGACAC ATGGTTCAGT TCGGGAATCT TTCCCTTCTC CGTTTTTGGC 
TGGCCAGACC AAACTAAGGA TCTGCAAACG TTCTATCCCA CATCGCTGTT GGAGACGGGT 
CACGACATTC TCTTCTTCTG GGTGGCTCGC ATGGTATTCT TTGGACAGAA GCTGCTGGGC 
AAACTGCCGT TTAAGGAGGT CTATCTTCAC CCCATGGTGA GGGATGCGCA CGGACGCAAA 
ATGTCCAAGT CCCTGGGAAA TGTTATCGAT CCTATGGATG TTATTCGTGG CATTACGCTC 
GAGGGACTGC ATGCTCAACT TGTGGGCTCC AACCTGGATC CTCGGGAGAT CGAAAAGGCT 
AAGGCTGGAC AGAAACAGGA TTATCCGCAG GGAATTCCCG AGTGCGGCTC GGATGCACTT 
CGATTCGCGC TGTGCGCCTA TATCACGCAA GCGCGCGACA TCAACCTGGA CATCAATCGC 
GTTCTGGGAT ATCGTTTCTT CTGCAACAAG CTGTGGAACG CCACCAAGTT CGCCCTGCTG 
TACTTCACCG GCTCTGAGAA GTTTGACACG GAGCTAAGTG CGTCTGCAGC GATCAACCAG 
ATGGACGCCT GGATCCTGTC ACGCTTGGCA GCTGCCATAG AAGCTTGCAA CACCGGCTTT 
GAGTCGTACG ACTTTGCTGC AGCGACCAGC GCCTGCTACG CATTCTGGCT CTACGATCTG 
TGCGACGTGT ATTTGGAGTG TCTGAAGCCG ATCTTCCAGA GCGGCAGCGA GGAGCAGCAG 
ACCGCCGCAA GGCGCACGCT CTACGTATGT CTAGATTATG GACTGCGCTT ACTCTCCCCG 
TTCATGCCTT TCATCACGGA GGAACTTTAC CAGCGGCTTC CGAGGGCAAA TCCCGCTCCT 
AGCATTTGCG TGGCTAGCTA TCCCAGCAAT ACATCCTGGC GCAGCACAAA GATAGAATCG 
GACGTTGAAT TTGTGCAGAA GGCGGCGCGA ATCATCCGAT CTGCCCGTTC CGACTATAAT 
CTGCCCAACA AGGTTAAAAC CGAGGTGTAT ATAGTATGCA CGGATTCGGT GCCCAGTGAA 
ATACTCAAAC GCTACGCCAG CGATCTGGCC ACAATCTCCT ACTGCTCCAA TGTGGTCTTT 
GACAGTCCCG CACCGCAGGG CTGTGCGATC CTCACCGTGA CGGGCCAGTG CGAGGTGCAC 
CTGCTGCTCA AGGGACTCGT GGAGGCGGAC AAGGAGATAG CCAAGCTGCA GAAGAAGAGC 
GACCAGCTGG TGCAGACGGT GGGCAAGCTA ACTCAGGCCA TCCAGGCCGC CGACTACGCA 
ACTAAGGTTC CCGCCGAGGT GCAGGAGGCC AACGAGACAA AACTCTGCGA GTCGCGGGCA 
GAGATCGAAC GCATCGCTGC CGCCATCGAG ACTCTGAAAC TAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ValRS ( NM_165970.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725259.1 Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12713
Clone ID Related Accession (Same CDS sequence)	NM_165970.2 , NM_080099.3
Accession Version	NM_165970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Valyl-tRNA synthetase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165970.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAGG CTGAACAGCA GAACGATGCC AACGCACCAG CCGGAGCCGG GGGTGATCCT 
CCCAAGACGG CCAAGCAACT GGAAAAGGAG CGTCTGAAGG CGGAGAAGCT GGCCAAGCTG 
CAGGCGAAGC TGGACAAGAA GGCTGCGGCT GCTCCAGCAG CGGGTGAGAA GAAGGAGAAG 
CCCGAGAAGC GCACCAAGGA GGTGAAAGAG GCTGCCGTGT ACACGGCCCA AACCGCGCCC 
GGTGAGAAGA AGGATCTGAG TGGAGCCTTG CCGGATGCCT ACAGTCCACG GTATGTGGAA 
GCACAGTGGT ACAGCTGGTG GGAGAAGGAG GGCTTCTTCA CGCCCGAGTA CGGACGCGCA 
TCCATAGACG CCCCCAATCC CAATGGCAAG TTTGTGATGA TCATTCCACC ACCAAACGTG 
ACGGGCTCTC TGCATTTGGG TCACGCCCTG ACCAACGCCA TCGAGGACGC CATCACGCGC 
TACCACCGCA TGAAGGGACG AACCACTCTA TGGGTGCCTG GTTGCGACCA CGCCGGCATC 
GCCACCCAGG TGGTGGTAGA GAAGCTATTG TGGCGCGACG AGAAGCTATC GCGGCACGAC 
CTAGGTCGCG AAAAGTTCAT TGAGCGCATC TGGGACTGGC GCCGGGAAAA GGGCGGTCGC 
ATCTATGAGC AGCTGAAGTC CCTGGGCTCC TCCTATGACT GGACCCGAGT GGCCTTCACC 
ATGGACCCCA AGCTCTGTCG CGCCGTTACC GAGGCTTTCG TCCGATTGCA CGAGGAGGGC 
AGCATCTACC GTAGCTCCCG ACTGGTCAAT TGGTCGTGCA CCCTACGATC GGCTATTTCG 
GACATTGAAG TGGACAAGGT GGAGATTCCC GGTCGCACAT TCCTCTCCAT TCCCGGATAC 
GAGGACAAAG TGGAATTTGG TGTTCTAATA AAGTTCGCCT ACAAGGTGGA AGGCAGTGAC 
GAAGAGATCA TTGTAGCCAC CACCCGTATC GAGACTATGC TGGGCGATAC TGCTGTGGCT 
GTGCATCCCC AAGACGATCG GTACAAGCAC CTGCATGGCA AGTTCGTAGT CCATCCGTTC 
TCCACGCGCC GCCTGCCAAT CGTCTGTGAC GAGTTCGTGG ACATGGCTTT CGGTACGGGT 
GCTGTGAAGA TCACCCCAGC CCACGATCCC AACGATTACG AGGTGGGCAA GCGCTGCAAT 
TTGCCCTTCA TTACAATCTT CAACGACGAC GGCTACATAA TCGGCGACTA CGGAGAGTTT 
ACTGGAATGA AGCGGTTCGA GTGCCGCAAG AAAATCCTGG AGAAGCTTAA GGCCCTGAAT 
CTATACCGCG AGACATTGAA CAACCCGATG GTGGTGCCCA TCTGCAGTCG CTCCAAGGAC 
GTGGTGGAGC CGCTGATTAA GCCGCAGTGG TATGTTAGTT GCTCGGACAT GGCAGCCTCT 
GCCACTGAGG CAGTGCGCTC CGGAGAACTA AAGATCATTC CGGAGCATCA CACCAAGACG 
TGGTACCACT GGATGGACGG TATTCGCGAC TGGTGCGTGT CGAGGCAGCT GTGGTGGGGT 
CACCGGATTC CGGCATACCA TGTCAGCTTC ACCGACCCTT CTCTTCAAAC TGGATCGAAC 
GACGACGAGC AGTACTGGAT TGTAGCACGC AGCGAAGCGG AGGCTCTGAC CAAGGCAGCC 
GAACGTTTTG GAGTGGACGC CAGCAAGATA GTACTGAAAC AAGACGAAGA TGTCCTGGAC 
ACATGGTTCA GTTCGGGAAT CTTTCCCTTC TCCGTTTTTG GCTGGCCAGA CCAAACTAAG 
GATCTGCAAA CGTTCTATCC CACATCGCTG TTGGAGACGG GTCACGACAT TCTCTTCTTC 
TGGGTGGCTC GCATGGTATT CTTTGGACAG AAGCTGCTGG GCAAACTGCC GTTTAAGGAG 
GTCTATCTTC ACCCCATGGT GAGGGATGCG CACGGACGCA AAATGTCCAA GTCCCTGGGA 
AATGTTATCG ATCCTATGGA TGTTATTCGT GGCATTACGC TCGAGGGACT GCATGCTCAA 
CTTGTGGGCT CCAACCTGGA TCCTCGGGAG ATCGAAAAGG CTAAGGCTGG ACAGAAACAG 
GATTATCCGC AGGGAATTCC CGAGTGCGGC TCGGATGCAC TTCGATTCGC GCTGTGCGCC 
TATATCACGC AAGCGCGCGA CATCAACCTG GACATCAATC GCGTTCTGGG ATATCGTTTC 
TTCTGCAACA AGCTGTGGAA CGCCACCAAG TTCGCCCTGC TGTACTTCAC CGGCTCTGAG 
AAGTTTGACA CGGAGCTAAG TGCGTCTGCA GCGATCAACC AGATGGACGC CTGGATCCTG 
TCACGCTTGG CAGCTGCCAT AGAAGCTTGC AACACCGGCT TTGAGTCGTA CGACTTTGCT 
GCAGCGACCA GCGCCTGCTA CGCATTCTGG CTCTACGATC TGTGCGACGT GTATTTGGAG 
TGTCTGAAGC CGATCTTCCA GAGCGGCAGC GAGGAGCAGC AGACCGCCGC AAGGCGCACG 
CTCTACGTAT GTCTAGATTA TGGACTGCGC TTACTCTCCC CGTTCATGCC TTTCATCACG 
GAGGAACTTT ACCAGCGGCT TCCGAGGGCA AATCCCGCTC CTAGCATTTG CGTGGCTAGC 
TATCCCAGCA ATACATCCTG GCGCAGCACA AAGATAGAAT CGGACGTTGA ATTTGTGCAG 
AAGGCGGCGC GAATCATCCG ATCTGCCCGT TCCGACTATA ATCTGCCCAA CAAGGTTAAA 
ACCGAGGTGT ATATAGTATG CACGGATTCG GTGCCCAGTG AAATACTCAA ACGCTACGCC 
AGCGATCTGG CCACAATCTC CTACTGCTCC AATGTGGTCT TTGACAGTCC CGCACCGCAG 
GGCTGTGCGA TCCTCACCGT GACGGGCCAG TGCGAGGTGC ACCTGCTGCT CAAGGGACTC 
GTGGAGGCGG ACAAGGAGAT AGCCAAGCTG CAGAAGAAGA GCGACCAGCT GGTGCAGACG 
GTGGGCAAGC TAACTCAGGC CATCCAGGCC GCCGACTACG CAACTAAGGT TCCCGCCGAG 
GTGCAGGAGG CCAACGAGAC AAAACTCTGC GAGTCGCGGG CAGAGATCGA ACGCATCGCT 
GCCGCCATCG AGACTCTGAA ACTAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725259.1
CDS	96..3245
Translation	MPEAEQQNDANAPAGAGGDPPKTAKQLEKERLKAEKLAKLQAKLDKKAAAAPAAGEKKEKPEKRTKEVKEAAVYTAQTAPGEKKDLSGALPDAYSPRYVEAQWYSWWEKEGFFTPEYGRASIDAPNPNGKFVMIIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLLWRDEKLSRHDLGREKFIERIWDWRREKGGRIYEQLKSLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLSIPGYEDKVEFGVLIKFAYKVEGSDEEIIVATTRIETMLGDTAVAVHPQDDRYKHLHGKFVVHPFSTRRLPIVCDEFVDMAFGTGAVKITPAHDPNDYEVGKRCNLPFITIFNDDGYIIGDYGEFTGMKRFECRKKILEKLKALNLYRETLNNPMVVPICSRSKDVVEPLIKPQWYVSCSDMAASATEAVRSGELKIIPEHHTKTWYHWMDGIRDWCVSRQLWWGHRIPAYHVSFTDPSLQTGSNDDEQYWIVARSEAEALTKAAERFGVDASKIVLKQDEDVLDTWFSSGIFPFSVFGWPDQTKDLQTFYPTSLLETGHDILFFWVARMVFFGQKLLGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPMDVIRGITLEGLHAQLVGSNLDPREIEKAKAGQKQDYPQGIPECGSDALRFALCAYITQARDINLDINRVLGYRFFCNKLWNATKFALLYFTGSEKFDTELSASAAINQMDAWILSRLAAAIEACNTGFESYDFAAATSACYAFWLYDLCDVYLECLKPIFQSGSEEQQTAARRTLYVCLDYGLRLLSPFMPFITEELYQRLPRANPAPSICVASYPSNTSWRSTKIESDVEFVQKAARIIRSARSDYNLPNKVKTEVYIVCTDSVPSEILKRYASDLATISYCSNVVFDSPAPQGCAILTVTGQCEVHLLLKGLVEADKEIAKLQKKSDQLVQTVGKLTQAIQAADYATKVPAEVQEANETKLCESRAEIERIAAAIETLKLM

Target ORF information:

RefSeq Version	NM_165970.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165970.2

ORF Insert Sequence:

ATGCCCGAGG CTGAACAGCA GAACGATGCC AACGCACCAG CCGGAGCCGG GGGTGATCCT 
CCCAAGACGG CCAAGCAACT GGAAAAGGAG CGTCTGAAGG CGGAGAAGCT GGCCAAGCTG 
CAGGCGAAGC TGGACAAGAA GGCTGCGGCT GCTCCAGCAG CGGGTGAGAA GAAGGAGAAG 
CCCGAGAAGC GCACCAAGGA GGTGAAAGAG GCTGCCGTGT ACACGGCCCA AACCGCGCCC 
GGTGAGAAGA AGGATCTGAG TGGAGCCTTG CCGGATGCCT ACAGTCCACG GTATGTGGAA 
GCACAGTGGT ACAGCTGGTG GGAGAAGGAG GGCTTCTTCA CGCCCGAGTA CGGACGCGCA 
TCCATAGACG CCCCCAATCC CAATGGCAAG TTTGTGATGA TCATTCCACC ACCAAACGTG 
ACGGGCTCTC TGCATTTGGG TCACGCCCTG ACCAACGCCA TCGAGGACGC CATCACGCGC 
TACCACCGCA TGAAGGGACG AACCACTCTA TGGGTGCCTG GTTGCGACCA CGCCGGCATC 
GCCACCCAGG TGGTGGTAGA GAAGCTATTG TGGCGCGACG AGAAGCTATC GCGGCACGAC 
CTAGGTCGCG AAAAGTTCAT TGAGCGCATC TGGGACTGGC GCCGGGAAAA GGGCGGTCGC 
ATCTATGAGC AGCTGAAGTC CCTGGGCTCC TCCTATGACT GGACCCGAGT GGCCTTCACC 
ATGGACCCCA AGCTCTGTCG CGCCGTTACC GAGGCTTTCG TCCGATTGCA CGAGGAGGGC 
AGCATCTACC GTAGCTCCCG ACTGGTCAAT TGGTCGTGCA CCCTACGATC GGCTATTTCG 
GACATTGAAG TGGACAAGGT GGAGATTCCC GGTCGCACAT TCCTCTCCAT TCCCGGATAC 
GAGGACAAAG TGGAATTTGG TGTTCTAATA AAGTTCGCCT ACAAGGTGGA AGGCAGTGAC 
GAAGAGATCA TTGTAGCCAC CACCCGTATC GAGACTATGC TGGGCGATAC TGCTGTGGCT 
GTGCATCCCC AAGACGATCG GTACAAGCAC CTGCATGGCA AGTTCGTAGT CCATCCGTTC 
TCCACGCGCC GCCTGCCAAT CGTCTGTGAC GAGTTCGTGG ACATGGCTTT CGGTACGGGT 
GCTGTGAAGA TCACCCCAGC CCACGATCCC AACGATTACG AGGTGGGCAA GCGCTGCAAT 
TTGCCCTTCA TTACAATCTT CAACGACGAC GGCTACATAA TCGGCGACTA CGGAGAGTTT 
ACTGGAATGA AGCGGTTCGA GTGCCGCAAG AAAATCCTGG AGAAGCTTAA GGCCCTGAAT 
CTATACCGCG AGACATTGAA CAACCCGATG GTGGTGCCCA TCTGCAGTCG CTCCAAGGAC 
GTGGTGGAGC CGCTGATTAA GCCGCAGTGG TATGTTAGTT GCTCGGACAT GGCAGCCTCT 
GCCACTGAGG CAGTGCGCTC CGGAGAACTA AAGATCATTC CGGAGCATCA CACCAAGACG 
TGGTACCACT GGATGGACGG TATTCGCGAC TGGTGCGTGT CGAGGCAGCT GTGGTGGGGT 
CACCGGATTC CGGCATACCA TGTCAGCTTC ACCGACCCTT CTCTTCAAAC TGGATCGAAC 
GACGACGAGC AGTACTGGAT TGTAGCACGC AGCGAAGCGG AGGCTCTGAC CAAGGCAGCC 
GAACGTTTTG GAGTGGACGC CAGCAAGATA GTACTGAAAC AAGACGAAGA TGTCCTGGAC 
ACATGGTTCA GTTCGGGAAT CTTTCCCTTC TCCGTTTTTG GCTGGCCAGA CCAAACTAAG 
GATCTGCAAA CGTTCTATCC CACATCGCTG TTGGAGACGG GTCACGACAT TCTCTTCTTC 
TGGGTGGCTC GCATGGTATT CTTTGGACAG AAGCTGCTGG GCAAACTGCC GTTTAAGGAG 
GTCTATCTTC ACCCCATGGT GAGGGATGCG CACGGACGCA AAATGTCCAA GTCCCTGGGA 
AATGTTATCG ATCCTATGGA TGTTATTCGT GGCATTACGC TCGAGGGACT GCATGCTCAA 
CTTGTGGGCT CCAACCTGGA TCCTCGGGAG ATCGAAAAGG CTAAGGCTGG ACAGAAACAG 
GATTATCCGC AGGGAATTCC CGAGTGCGGC TCGGATGCAC TTCGATTCGC GCTGTGCGCC 
TATATCACGC AAGCGCGCGA CATCAACCTG GACATCAATC GCGTTCTGGG ATATCGTTTC 
TTCTGCAACA AGCTGTGGAA CGCCACCAAG TTCGCCCTGC TGTACTTCAC CGGCTCTGAG 
AAGTTTGACA CGGAGCTAAG TGCGTCTGCA GCGATCAACC AGATGGACGC CTGGATCCTG 
TCACGCTTGG CAGCTGCCAT AGAAGCTTGC AACACCGGCT TTGAGTCGTA CGACTTTGCT 
GCAGCGACCA GCGCCTGCTA CGCATTCTGG CTCTACGATC TGTGCGACGT GTATTTGGAG 
TGTCTGAAGC CGATCTTCCA GAGCGGCAGC GAGGAGCAGC AGACCGCCGC AAGGCGCACG 
CTCTACGTAT GTCTAGATTA TGGACTGCGC TTACTCTCCC CGTTCATGCC TTTCATCACG 
GAGGAACTTT ACCAGCGGCT TCCGAGGGCA AATCCCGCTC CTAGCATTTG CGTGGCTAGC 
TATCCCAGCA ATACATCCTG GCGCAGCACA AAGATAGAAT CGGACGTTGA ATTTGTGCAG 
AAGGCGGCGC GAATCATCCG ATCTGCCCGT TCCGACTATA ATCTGCCCAA CAAGGTTAAA 
ACCGAGGTGT ATATAGTATG CACGGATTCG GTGCCCAGTG AAATACTCAA ACGCTACGCC 
AGCGATCTGG CCACAATCTC CTACTGCTCC AATGTGGTCT TTGACAGTCC CGCACCGCAG 
GGCTGTGCGA TCCTCACCGT GACGGGCCAG TGCGAGGTGC ACCTGCTGCT CAAGGGACTC 
GTGGAGGCGG ACAAGGAGAT AGCCAAGCTG CAGAAGAAGA GCGACCAGCT GGTGCAGACG 
GTGGGCAAGC TAACTCAGGC CATCCAGGCC GCCGACTACG CAACTAAGGT TCCCGCCGAG 
GTGCAGGAGG CCAACGAGAC AAAACTCTGC GAGTCGCGGG CAGAGATCGA ACGCATCGCT 
GCCGCCATCG AGACTCTGAA ACTAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ValRS ( NM_080099.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524838.1 Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant A, mRNA.

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CloneID	OFa12713
Clone ID Related Accession (Same CDS sequence)	NM_165970.2 , NM_080099.3
Accession Version	NM_080099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Valyl-tRNA synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_080099.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAGG CTGAACAGCA GAACGATGCC AACGCACCAG CCGGAGCCGG GGGTGATCCT 
CCCAAGACGG CCAAGCAACT GGAAAAGGAG CGTCTGAAGG CGGAGAAGCT GGCCAAGCTG 
CAGGCGAAGC TGGACAAGAA GGCTGCGGCT GCTCCAGCAG CGGGTGAGAA GAAGGAGAAG 
CCCGAGAAGC GCACCAAGGA GGTGAAAGAG GCTGCCGTGT ACACGGCCCA AACCGCGCCC 
GGTGAGAAGA AGGATCTGAG TGGAGCCTTG CCGGATGCCT ACAGTCCACG GTATGTGGAA 
GCACAGTGGT ACAGCTGGTG GGAGAAGGAG GGCTTCTTCA CGCCCGAGTA CGGACGCGCA 
TCCATAGACG CCCCCAATCC CAATGGCAAG TTTGTGATGA TCATTCCACC ACCAAACGTG 
ACGGGCTCTC TGCATTTGGG TCACGCCCTG ACCAACGCCA TCGAGGACGC CATCACGCGC 
TACCACCGCA TGAAGGGACG AACCACTCTA TGGGTGCCTG GTTGCGACCA CGCCGGCATC 
GCCACCCAGG TGGTGGTAGA GAAGCTATTG TGGCGCGACG AGAAGCTATC GCGGCACGAC 
CTAGGTCGCG AAAAGTTCAT TGAGCGCATC TGGGACTGGC GCCGGGAAAA GGGCGGTCGC 
ATCTATGAGC AGCTGAAGTC CCTGGGCTCC TCCTATGACT GGACCCGAGT GGCCTTCACC 
ATGGACCCCA AGCTCTGTCG CGCCGTTACC GAGGCTTTCG TCCGATTGCA CGAGGAGGGC 
AGCATCTACC GTAGCTCCCG ACTGGTCAAT TGGTCGTGCA CCCTACGATC GGCTATTTCG 
GACATTGAAG TGGACAAGGT GGAGATTCCC GGTCGCACAT TCCTCTCCAT TCCCGGATAC 
GAGGACAAAG TGGAATTTGG TGTTCTAATA AAGTTCGCCT ACAAGGTGGA AGGCAGTGAC 
GAAGAGATCA TTGTAGCCAC CACCCGTATC GAGACTATGC TGGGCGATAC TGCTGTGGCT 
GTGCATCCCC AAGACGATCG GTACAAGCAC CTGCATGGCA AGTTCGTAGT CCATCCGTTC 
TCCACGCGCC GCCTGCCAAT CGTCTGTGAC GAGTTCGTGG ACATGGCTTT CGGTACGGGT 
GCTGTGAAGA TCACCCCAGC CCACGATCCC AACGATTACG AGGTGGGCAA GCGCTGCAAT 
TTGCCCTTCA TTACAATCTT CAACGACGAC GGCTACATAA TCGGCGACTA CGGAGAGTTT 
ACTGGAATGA AGCGGTTCGA GTGCCGCAAG AAAATCCTGG AGAAGCTTAA GGCCCTGAAT 
CTATACCGCG AGACATTGAA CAACCCGATG GTGGTGCCCA TCTGCAGTCG CTCCAAGGAC 
GTGGTGGAGC CGCTGATTAA GCCGCAGTGG TATGTTAGTT GCTCGGACAT GGCAGCCTCT 
GCCACTGAGG CAGTGCGCTC CGGAGAACTA AAGATCATTC CGGAGCATCA CACCAAGACG 
TGGTACCACT GGATGGACGG TATTCGCGAC TGGTGCGTGT CGAGGCAGCT GTGGTGGGGT 
CACCGGATTC CGGCATACCA TGTCAGCTTC ACCGACCCTT CTCTTCAAAC TGGATCGAAC 
GACGACGAGC AGTACTGGAT TGTAGCACGC AGCGAAGCGG AGGCTCTGAC CAAGGCAGCC 
GAACGTTTTG GAGTGGACGC CAGCAAGATA GTACTGAAAC AAGACGAAGA TGTCCTGGAC 
ACATGGTTCA GTTCGGGAAT CTTTCCCTTC TCCGTTTTTG GCTGGCCAGA CCAAACTAAG 
GATCTGCAAA CGTTCTATCC CACATCGCTG TTGGAGACGG GTCACGACAT TCTCTTCTTC 
TGGGTGGCTC GCATGGTATT CTTTGGACAG AAGCTGCTGG GCAAACTGCC GTTTAAGGAG 
GTCTATCTTC ACCCCATGGT GAGGGATGCG CACGGACGCA AAATGTCCAA GTCCCTGGGA 
AATGTTATCG ATCCTATGGA TGTTATTCGT GGCATTACGC TCGAGGGACT GCATGCTCAA 
CTTGTGGGCT CCAACCTGGA TCCTCGGGAG ATCGAAAAGG CTAAGGCTGG ACAGAAACAG 
GATTATCCGC AGGGAATTCC CGAGTGCGGC TCGGATGCAC TTCGATTCGC GCTGTGCGCC 
TATATCACGC AAGCGCGCGA CATCAACCTG GACATCAATC GCGTTCTGGG ATATCGTTTC 
TTCTGCAACA AGCTGTGGAA CGCCACCAAG TTCGCCCTGC TGTACTTCAC CGGCTCTGAG 
AAGTTTGACA CGGAGCTAAG TGCGTCTGCA GCGATCAACC AGATGGACGC CTGGATCCTG 
TCACGCTTGG CAGCTGCCAT AGAAGCTTGC AACACCGGCT TTGAGTCGTA CGACTTTGCT 
GCAGCGACCA GCGCCTGCTA CGCATTCTGG CTCTACGATC TGTGCGACGT GTATTTGGAG 
TGTCTGAAGC CGATCTTCCA GAGCGGCAGC GAGGAGCAGC AGACCGCCGC AAGGCGCACG 
CTCTACGTAT GTCTAGATTA TGGACTGCGC TTACTCTCCC CGTTCATGCC TTTCATCACG 
GAGGAACTTT ACCAGCGGCT TCCGAGGGCA AATCCCGCTC CTAGCATTTG CGTGGCTAGC 
TATCCCAGCA ATACATCCTG GCGCAGCACA AAGATAGAAT CGGACGTTGA ATTTGTGCAG 
AAGGCGGCGC GAATCATCCG ATCTGCCCGT TCCGACTATA ATCTGCCCAA CAAGGTTAAA 
ACCGAGGTGT ATATAGTATG CACGGATTCG GTGCCCAGTG AAATACTCAA ACGCTACGCC 
AGCGATCTGG CCACAATCTC CTACTGCTCC AATGTGGTCT TTGACAGTCC CGCACCGCAG 
GGCTGTGCGA TCCTCACCGT GACGGGCCAG TGCGAGGTGC ACCTGCTGCT CAAGGGACTC 
GTGGAGGCGG ACAAGGAGAT AGCCAAGCTG CAGAAGAAGA GCGACCAGCT GGTGCAGACG 
GTGGGCAAGC TAACTCAGGC CATCCAGGCC GCCGACTACG CAACTAAGGT TCCCGCCGAG 
GTGCAGGAGG CCAACGAGAC AAAACTCTGC GAGTCGCGGG CAGAGATCGA ACGCATCGCT 
GCCGCCATCG AGACTCTGAA ACTAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524838.1
CDS	60..3209
Translation	MPEAEQQNDANAPAGAGGDPPKTAKQLEKERLKAEKLAKLQAKLDKKAAAAPAAGEKKEKPEKRTKEVKEAAVYTAQTAPGEKKDLSGALPDAYSPRYVEAQWYSWWEKEGFFTPEYGRASIDAPNPNGKFVMIIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLLWRDEKLSRHDLGREKFIERIWDWRREKGGRIYEQLKSLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLSIPGYEDKVEFGVLIKFAYKVEGSDEEIIVATTRIETMLGDTAVAVHPQDDRYKHLHGKFVVHPFSTRRLPIVCDEFVDMAFGTGAVKITPAHDPNDYEVGKRCNLPFITIFNDDGYIIGDYGEFTGMKRFECRKKILEKLKALNLYRETLNNPMVVPICSRSKDVVEPLIKPQWYVSCSDMAASATEAVRSGELKIIPEHHTKTWYHWMDGIRDWCVSRQLWWGHRIPAYHVSFTDPSLQTGSNDDEQYWIVARSEAEALTKAAERFGVDASKIVLKQDEDVLDTWFSSGIFPFSVFGWPDQTKDLQTFYPTSLLETGHDILFFWVARMVFFGQKLLGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPMDVIRGITLEGLHAQLVGSNLDPREIEKAKAGQKQDYPQGIPECGSDALRFALCAYITQARDINLDINRVLGYRFFCNKLWNATKFALLYFTGSEKFDTELSASAAINQMDAWILSRLAAAIEACNTGFESYDFAAATSACYAFWLYDLCDVYLECLKPIFQSGSEEQQTAARRTLYVCLDYGLRLLSPFMPFITEELYQRLPRANPAPSICVASYPSNTSWRSTKIESDVEFVQKAARIIRSARSDYNLPNKVKTEVYIVCTDSVPSEILKRYASDLATISYCSNVVFDSPAPQGCAILTVTGQCEVHLLLKGLVEADKEIAKLQKKSDQLVQTVGKLTQAIQAADYATKVPAEVQEANETKLCESRAEIERIAAAIETLKLM

Target ORF information:

RefSeq Version	NM_080099.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Valyl-tRNA synthetase (ValRS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080099.3

ORF Insert Sequence:

ATGCCCGAGG CTGAACAGCA GAACGATGCC AACGCACCAG CCGGAGCCGG GGGTGATCCT 
CCCAAGACGG CCAAGCAACT GGAAAAGGAG CGTCTGAAGG CGGAGAAGCT GGCCAAGCTG 
CAGGCGAAGC TGGACAAGAA GGCTGCGGCT GCTCCAGCAG CGGGTGAGAA GAAGGAGAAG 
CCCGAGAAGC GCACCAAGGA GGTGAAAGAG GCTGCCGTGT ACACGGCCCA AACCGCGCCC 
GGTGAGAAGA AGGATCTGAG TGGAGCCTTG CCGGATGCCT ACAGTCCACG GTATGTGGAA 
GCACAGTGGT ACAGCTGGTG GGAGAAGGAG GGCTTCTTCA CGCCCGAGTA CGGACGCGCA 
TCCATAGACG CCCCCAATCC CAATGGCAAG TTTGTGATGA TCATTCCACC ACCAAACGTG 
ACGGGCTCTC TGCATTTGGG TCACGCCCTG ACCAACGCCA TCGAGGACGC CATCACGCGC 
TACCACCGCA TGAAGGGACG AACCACTCTA TGGGTGCCTG GTTGCGACCA CGCCGGCATC 
GCCACCCAGG TGGTGGTAGA GAAGCTATTG TGGCGCGACG AGAAGCTATC GCGGCACGAC 
CTAGGTCGCG AAAAGTTCAT TGAGCGCATC TGGGACTGGC GCCGGGAAAA GGGCGGTCGC 
ATCTATGAGC AGCTGAAGTC CCTGGGCTCC TCCTATGACT GGACCCGAGT GGCCTTCACC 
ATGGACCCCA AGCTCTGTCG CGCCGTTACC GAGGCTTTCG TCCGATTGCA CGAGGAGGGC 
AGCATCTACC GTAGCTCCCG ACTGGTCAAT TGGTCGTGCA CCCTACGATC GGCTATTTCG 
GACATTGAAG TGGACAAGGT GGAGATTCCC GGTCGCACAT TCCTCTCCAT TCCCGGATAC 
GAGGACAAAG TGGAATTTGG TGTTCTAATA AAGTTCGCCT ACAAGGTGGA AGGCAGTGAC 
GAAGAGATCA TTGTAGCCAC CACCCGTATC GAGACTATGC TGGGCGATAC TGCTGTGGCT 
GTGCATCCCC AAGACGATCG GTACAAGCAC CTGCATGGCA AGTTCGTAGT CCATCCGTTC 
TCCACGCGCC GCCTGCCAAT CGTCTGTGAC GAGTTCGTGG ACATGGCTTT CGGTACGGGT 
GCTGTGAAGA TCACCCCAGC CCACGATCCC AACGATTACG AGGTGGGCAA GCGCTGCAAT 
TTGCCCTTCA TTACAATCTT CAACGACGAC GGCTACATAA TCGGCGACTA CGGAGAGTTT 
ACTGGAATGA AGCGGTTCGA GTGCCGCAAG AAAATCCTGG AGAAGCTTAA GGCCCTGAAT 
CTATACCGCG AGACATTGAA CAACCCGATG GTGGTGCCCA TCTGCAGTCG CTCCAAGGAC 
GTGGTGGAGC CGCTGATTAA GCCGCAGTGG TATGTTAGTT GCTCGGACAT GGCAGCCTCT 
GCCACTGAGG CAGTGCGCTC CGGAGAACTA AAGATCATTC CGGAGCATCA CACCAAGACG 
TGGTACCACT GGATGGACGG TATTCGCGAC TGGTGCGTGT CGAGGCAGCT GTGGTGGGGT 
CACCGGATTC CGGCATACCA TGTCAGCTTC ACCGACCCTT CTCTTCAAAC TGGATCGAAC 
GACGACGAGC AGTACTGGAT TGTAGCACGC AGCGAAGCGG AGGCTCTGAC CAAGGCAGCC 
GAACGTTTTG GAGTGGACGC CAGCAAGATA GTACTGAAAC AAGACGAAGA TGTCCTGGAC 
ACATGGTTCA GTTCGGGAAT CTTTCCCTTC TCCGTTTTTG GCTGGCCAGA CCAAACTAAG 
GATCTGCAAA CGTTCTATCC CACATCGCTG TTGGAGACGG GTCACGACAT TCTCTTCTTC 
TGGGTGGCTC GCATGGTATT CTTTGGACAG AAGCTGCTGG GCAAACTGCC GTTTAAGGAG 
GTCTATCTTC ACCCCATGGT GAGGGATGCG CACGGACGCA AAATGTCCAA GTCCCTGGGA 
AATGTTATCG ATCCTATGGA TGTTATTCGT GGCATTACGC TCGAGGGACT GCATGCTCAA 
CTTGTGGGCT CCAACCTGGA TCCTCGGGAG ATCGAAAAGG CTAAGGCTGG ACAGAAACAG 
GATTATCCGC AGGGAATTCC CGAGTGCGGC TCGGATGCAC TTCGATTCGC GCTGTGCGCC 
TATATCACGC AAGCGCGCGA CATCAACCTG GACATCAATC GCGTTCTGGG ATATCGTTTC 
TTCTGCAACA AGCTGTGGAA CGCCACCAAG TTCGCCCTGC TGTACTTCAC CGGCTCTGAG 
AAGTTTGACA CGGAGCTAAG TGCGTCTGCA GCGATCAACC AGATGGACGC CTGGATCCTG 
TCACGCTTGG CAGCTGCCAT AGAAGCTTGC AACACCGGCT TTGAGTCGTA CGACTTTGCT 
GCAGCGACCA GCGCCTGCTA CGCATTCTGG CTCTACGATC TGTGCGACGT GTATTTGGAG 
TGTCTGAAGC CGATCTTCCA GAGCGGCAGC GAGGAGCAGC AGACCGCCGC AAGGCGCACG 
CTCTACGTAT GTCTAGATTA TGGACTGCGC TTACTCTCCC CGTTCATGCC TTTCATCACG 
GAGGAACTTT ACCAGCGGCT TCCGAGGGCA AATCCCGCTC CTAGCATTTG CGTGGCTAGC 
TATCCCAGCA ATACATCCTG GCGCAGCACA AAGATAGAAT CGGACGTTGA ATTTGTGCAG 
AAGGCGGCGC GAATCATCCG ATCTGCCCGT TCCGACTATA ATCTGCCCAA CAAGGTTAAA 
ACCGAGGTGT ATATAGTATG CACGGATTCG GTGCCCAGTG AAATACTCAA ACGCTACGCC 
AGCGATCTGG CCACAATCTC CTACTGCTCC AATGTGGTCT TTGACAGTCC CGCACCGCAG 
GGCTGTGCGA TCCTCACCGT GACGGGCCAG TGCGAGGTGC ACCTGCTGCT CAAGGGACTC 
GTGGAGGCGG ACAAGGAGAT AGCCAAGCTG CAGAAGAAGA GCGACCAGCT GGTGCAGACG 
GTGGGCAAGC TAACTCAGGC CATCCAGGCC GCCGACTACG CAACTAAGGT TCCCGCCGAG 
GTGCAGGAGG CCAACGAGAC AAAACTCTGC GAGTCGCGGG CAGAGATCGA ACGCATCGCT 
GCCGCCATCG AGACTCTGAA ACTAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ValRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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