VARS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS
Entrez Gene ID	505556
Full Name	valyl-tRNA synthetase
General protein information	Preferred Names valyl-tRNA synthetase Names valyl-tRNA synthetase valine--tRNA ligase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984080.1	XP_024839848.1	valine--tRNA ligase isoform X1
XM_024984082.1	XP_024839850.1	valine--tRNA ligase isoform X3
XM_002697347.2	XP_002697393.2	valine--tRNA ligase
XM_024984081.1	XP_024839849.1	valine--tRNA ligase isoform X2
XM_015468940.1	XP_015324426.1	valine--tRNA ligase
XM_024984083.1	XP_024839851.1	valine--tRNA ligase isoform X4

Pathways from BioSystems

Homologies

	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Neurospora crassa	NCU01965	XP_964329.2
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1

Gene Ontology

The following VARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa32187	XM_002697347.2 Latest version!	Bos taurus valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa32187	XM_015468940.1 Latest version!	Bos taurus valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa229798	XM_024984080.1 Latest version!	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229799	XM_024984081.1 Latest version!	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229800	XM_024984082.1 Latest version!	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa229801	XM_024984083.1 Latest version!	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS ( XM_002697347.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697393.2 Bos taurus valyl-tRNA synthetase (VARS), mRNA.

Price & Availability↑

CloneID	OBa32187
Clone ID Related Accession (Same CDS sequence)	XM_002697347.2 , XM_015468940.1
Accession Version	XM_002697347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 5, 2013 this sequence version replaced XM_002697347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACT CCTACAGCCC TCAGTACGTG GAGGCTGCCT GGTACCCTTG GTGGGAGCAG 
CAGGGCTTCT TCAGGCCTGA GTATGGGCGT TCCAGCGTGT CAGCACCAAA CCCCCGGGGC 
ATCTTCATGA TGTGCATCCC ACCCCCCAAC GTGACAGGCT CCCTGCACCT GGGCCATGCG 
CTCACCAACG CCATCCAGGA CTCCCTGACC CGCTGGCACC GCATGCGCGG AGAGACCACC 
CTGTGGAACC CTGGCTGTGA CCACGCGGGC ATTGCCACCC AGGTGGTGGT GGAGAAGAAA 
CTCTGGCGCG AGCAGGGGCT GAGTCGGCAC CAGCTGGGGC GAGAGGCTTT CCTGCAGGAG 
GTCTGGAAGT GGAAGGAGGA GAAAGGTGAC CGGATTTACC ACCAGCTGAA GAAGCTTGGC 
AGCTCCTTGG ACTGGAATCG AGCCTGTTTC ACCATGGATC CTAAACTGTC AGCAGCCGTG 
ACGGAGGCCT TTGTACGGCT CCACGAGGAG GGAATCATTT ACCGCAGCAC CCGCCTTGTC 
AACTGGTCCT GCACCCTCAA CTCTGCCATC TCCGACATCG AGGTGGATAA GAAGGAGCTG 
ACAGGTCGCA CACTGCTCTC CGTGCCTGGC TACAAAGAGA AGGTGGAGTT TGGGGTCCTT 
GTCTCCTTTG CTTACAAGGT CCAAGGCTCA GACAGCAACG ACGAGGTGGT AGTGGCAACC 
ACTCGGATCG AGACGATGCT GGGAGATGTG GCCGTAGCTG TGCATCCCAA AGACCCCAGA 
TACCAGCACC TGAAGGGCAA GAGCGTGATC CACCCATTTG TGTCTCGGAG CCTCCCCATC 
GTCTTTGATG ACTTTGTGGA CATGGAGTTC GGCACAGGTG CGGTGAAGAT CACCCCCGCC 
CACGACCAGA ATGACTATGA GGTTGGGCAG CGGCACGGGC TGGAGGCTGT CAGCATTATG 
GACGCCCGCG GGGCCCTCGT CAACGTGCCC CCACCTTTCC TGGGCCTGCC CAGGTTTGAG 
GCCAGAAAGG CGGTGCTGGC AGCGCTCAAG GAGCAGGGGC TGTTCCGCGG CATTGAGGAC 
AACCCCATGG TGGTGCCACT TTGCAACCGC TCCAAGGACG TGGTGGAGCC TCTGCTGCGG 
CCGCAGTGGT ACGTGCGCTG TGGGGAGATG GCTCAGGCCG CCAGCGCTGC CGTGACACGG 
GGTGACCTCC GCATCCTCCC GGAGGCCCAT CAGCGGACGT GGCACGCCTG GATGGACAAC 
ATCCGGGACT GGTGTATCTC CCGGCAGCTG TGGTGGGGTC ATCGCATCCC AGCCTACTTC 
ATCACTGTCA ATGACCCCGC CGTGCCCCCA GGGGAGGACC CTGACGGGCG GTACTGGGTG 
AGCGGGCGCA CTGAGGCCGA GGCCCGGGAG AAGGCAGCCA AGGAGTTCTG CGTGTCCCCT 
GACAAGATCA GTCTCCAGCA AGATGAAGAT GTACTGGACA CCTGGTTCTC CTCTGGCCTC 
TTCCCCTTCT CCATCCTAGG CTGGCCCAAC CAGTCAGAAG ATCTGAGTGT GTTCTACCCG 
GGGACGCTGC TGGAGACAGG CCATGACATC CTCTTCTTCT GGGTGGCCAG GATGGTCATG 
CTTGGCCTCA AGCTCACTGG CAAGCTGCCC TTCAAAGAGG TCTACCTCCA TGCCATCGTG 
CGGGACGCCC ACGGCCGGAA GATGAGCAAG TCTCTAGGCA ATGTCATTGA CCCCCTGGAC 
GTTATCCATG GGGTCACCCT GCAGGGTCTC CACGACCAGT TACTGAATAG CAACCTGGAT 
CCCAGCGAGG TGGAGAAGGC AAAAGAGGGG CAGAAGGCAG ATTTCCCGAC GGGGATCCCC 
GAGTGTGGCA CCGATGCGCT CCGGTTTGGA CTATGCGCCT ACACGTCCCA GGGCCGTGAC 
ATCAACCTGG ATGTGAACCG GATATTGGGG TACCGCCATT TCTGCAACAA GCTCTGGAAC 
GCCACCAAGT TTGCCCTCCG TGGCCTTGGG AAGGGTTTCG TGCCCTCACC CACGTCTGAG 
CCTGGAGGCC GCGAGAGTCT GGTGGACCGC TGGATCCGCA GCCGGCTGAC CGAAGCTGTG 
AGGCTCAGTA ACCAAGGTTT CCAGGCCTAC GACTTCCCGG CTGTCACCAC CGCCCAGTAC 
AGCTTCTGGC TCTATGAGCT CTGTGATGTC TACCTGGAGT GCCTTAAGCC TGTGCTGAAT 
GGGGTGGACC AGGTAGCAGC CGAGTGCGCC CGCCAGACCC TCTACACCTG CCTGGACGTG 
GGCCTGCGGC TACTCTCACC TTTCATGCCC TTCGTGACTG AGGAGCTGTT CCAGCGGCTG 
CCCCGGCGGA CACCACAAGC TCCCCCTAGC CTCTGCGTCA CTGCCTACCC AGAGCCCTCA 
GAGTGCTCCT GGAAGGACCC TGAGGCAGAA GCTGCCTTCG AGCTGGCCCT GAGCATCACT 
CGAGCCGTGC GCTCCCTGCG TGCCGACTAC AACCTTACCC GGATCCGGCC TGACTGTTTC 
CTGGAAGTGG CTGACGAGGC CACAGGTGCC CTGGCATCAG CGGTGTCGGG CTACGTGCAG 
ACACTGGCCA GCGCGGGGAT TGTGGCCGTC CTGGCGCTGG GGGCTTCTGC ACCCCAGGGC 
TGCGCTGTGG CCCTGGCCTC TGACCGCTGC TCCGTCCACC TGCAGCTGCA GGGGCTAGTA 
GACCCAGCCC GGGAGCTGGG CAAACTGCAG ACCAAGCGTG ACGAGGCGCA GCGGCAGGCC 
CAGCGTCTGC GGGAGCGCCG TGCCGCCTCA GGCTACACTG TCAAGGTGCC CCTCAAAGTC 
CAGGAGGCAG ATGAAGCCAA GCTCCAGCAG ACAGAAGCAG AGCTCAGGAA GGTTGATGAG 
GCCATTGCCC TGTTTCAGAA GATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697393.2
CDS	89..2998
Translation	MPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSNDEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAVSIMDARGALVNVPPPFLGLPRFEARKAVLAALKEQGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRTEAEAREKAAKEFCVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVTLQGLHDQLLNSNLDPSEVEKAKEGQKADFPTGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGRESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTAYPEPSECSWKDPEAEAAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRDEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_002697347.2
Organism	Bos taurus(cattle)
Definition	Bos taurus valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697347.2

ORF Insert Sequence:

ATGCCTGACT CCTACAGCCC TCAGTACGTG GAGGCTGCCT GGTACCCTTG GTGGGAGCAG 
CAGGGCTTCT TCAGGCCTGA GTATGGGCGT TCCAGCGTGT CAGCACCAAA CCCCCGGGGC 
ATCTTCATGA TGTGCATCCC ACCCCCCAAC GTGACAGGCT CCCTGCACCT GGGCCATGCG 
CTCACCAACG CCATCCAGGA CTCCCTGACC CGCTGGCACC GCATGCGCGG AGAGACCACC 
CTGTGGAACC CTGGCTGTGA CCACGCGGGC ATTGCCACCC AGGTGGTGGT GGAGAAGAAA 
CTCTGGCGCG AGCAGGGGCT GAGTCGGCAC CAGCTGGGGC GAGAGGCTTT CCTGCAGGAG 
GTCTGGAAGT GGAAGGAGGA GAAAGGTGAC CGGATTTACC ACCAGCTGAA GAAGCTTGGC 
AGCTCCTTGG ACTGGAATCG AGCCTGTTTC ACCATGGATC CTAAACTGTC AGCAGCCGTG 
ACGGAGGCCT TTGTACGGCT CCACGAGGAG GGAATCATTT ACCGCAGCAC CCGCCTTGTC 
AACTGGTCCT GCACCCTCAA CTCTGCCATC TCCGACATCG AGGTGGATAA GAAGGAGCTG 
ACAGGTCGCA CACTGCTCTC CGTGCCTGGC TACAAAGAGA AGGTGGAGTT TGGGGTCCTT 
GTCTCCTTTG CTTACAAGGT CCAAGGCTCA GACAGCAACG ACGAGGTGGT AGTGGCAACC 
ACTCGGATCG AGACGATGCT GGGAGATGTG GCCGTAGCTG TGCATCCCAA AGACCCCAGA 
TACCAGCACC TGAAGGGCAA GAGCGTGATC CACCCATTTG TGTCTCGGAG CCTCCCCATC 
GTCTTTGATG ACTTTGTGGA CATGGAGTTC GGCACAGGTG CGGTGAAGAT CACCCCCGCC 
CACGACCAGA ATGACTATGA GGTTGGGCAG CGGCACGGGC TGGAGGCTGT CAGCATTATG 
GACGCCCGCG GGGCCCTCGT CAACGTGCCC CCACCTTTCC TGGGCCTGCC CAGGTTTGAG 
GCCAGAAAGG CGGTGCTGGC AGCGCTCAAG GAGCAGGGGC TGTTCCGCGG CATTGAGGAC 
AACCCCATGG TGGTGCCACT TTGCAACCGC TCCAAGGACG TGGTGGAGCC TCTGCTGCGG 
CCGCAGTGGT ACGTGCGCTG TGGGGAGATG GCTCAGGCCG CCAGCGCTGC CGTGACACGG 
GGTGACCTCC GCATCCTCCC GGAGGCCCAT CAGCGGACGT GGCACGCCTG GATGGACAAC 
ATCCGGGACT GGTGTATCTC CCGGCAGCTG TGGTGGGGTC ATCGCATCCC AGCCTACTTC 
ATCACTGTCA ATGACCCCGC CGTGCCCCCA GGGGAGGACC CTGACGGGCG GTACTGGGTG 
AGCGGGCGCA CTGAGGCCGA GGCCCGGGAG AAGGCAGCCA AGGAGTTCTG CGTGTCCCCT 
GACAAGATCA GTCTCCAGCA AGATGAAGAT GTACTGGACA CCTGGTTCTC CTCTGGCCTC 
TTCCCCTTCT CCATCCTAGG CTGGCCCAAC CAGTCAGAAG ATCTGAGTGT GTTCTACCCG 
GGGACGCTGC TGGAGACAGG CCATGACATC CTCTTCTTCT GGGTGGCCAG GATGGTCATG 
CTTGGCCTCA AGCTCACTGG CAAGCTGCCC TTCAAAGAGG TCTACCTCCA TGCCATCGTG 
CGGGACGCCC ACGGCCGGAA GATGAGCAAG TCTCTAGGCA ATGTCATTGA CCCCCTGGAC 
GTTATCCATG GGGTCACCCT GCAGGGTCTC CACGACCAGT TACTGAATAG CAACCTGGAT 
CCCAGCGAGG TGGAGAAGGC AAAAGAGGGG CAGAAGGCAG ATTTCCCGAC GGGGATCCCC 
GAGTGTGGCA CCGATGCGCT CCGGTTTGGA CTATGCGCCT ACACGTCCCA GGGCCGTGAC 
ATCAACCTGG ATGTGAACCG GATATTGGGG TACCGCCATT TCTGCAACAA GCTCTGGAAC 
GCCACCAAGT TTGCCCTCCG TGGCCTTGGG AAGGGTTTCG TGCCCTCACC CACGTCTGAG 
CCTGGAGGCC GCGAGAGTCT GGTGGACCGC TGGATCCGCA GCCGGCTGAC CGAAGCTGTG 
AGGCTCAGTA ACCAAGGTTT CCAGGCCTAC GACTTCCCGG CTGTCACCAC CGCCCAGTAC 
AGCTTCTGGC TCTATGAGCT CTGTGATGTC TACCTGGAGT GCCTTAAGCC TGTGCTGAAT 
GGGGTGGACC AGGTAGCAGC CGAGTGCGCC CGCCAGACCC TCTACACCTG CCTGGACGTG 
GGCCTGCGGC TACTCTCACC TTTCATGCCC TTCGTGACTG AGGAGCTGTT CCAGCGGCTG 
CCCCGGCGGA CACCACAAGC TCCCCCTAGC CTCTGCGTCA CTGCCTACCC AGAGCCCTCA 
GAGTGCTCCT GGAAGGACCC TGAGGCAGAA GCTGCCTTCG AGCTGGCCCT GAGCATCACT 
CGAGCCGTGC GCTCCCTGCG TGCCGACTAC AACCTTACCC GGATCCGGCC TGACTGTTTC 
CTGGAAGTGG CTGACGAGGC CACAGGTGCC CTGGCATCAG CGGTGTCGGG CTACGTGCAG 
ACACTGGCCA GCGCGGGGAT TGTGGCCGTC CTGGCGCTGG GGGCTTCTGC ACCCCAGGGC 
TGCGCTGTGG CCCTGGCCTC TGACCGCTGC TCCGTCCACC TGCAGCTGCA GGGGCTAGTA 
GACCCAGCCC GGGAGCTGGG CAAACTGCAG ACCAAGCGTG ACGAGGCGCA GCGGCAGGCC 
CAGCGTCTGC GGGAGCGCCG TGCCGCCTCA GGCTACACTG TCAAGGTGCC CCTCAAAGTC 
CAGGAGGCAG ATGAAGCCAA GCTCCAGCAG ACAGAAGCAG AGCTCAGGAA GGTTGATGAG 
GCCATTGCCC TGTTTCAGAA GATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_015468940.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324426.1 Bos taurus valyl-tRNA synthetase (VARS), mRNA.

Price & Availability↑

CloneID	OBa32187
Clone ID Related Accession (Same CDS sequence)	XM_002697347.2 , XM_015468940.1
Accession Version	XM_015468940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646599.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACT CCTACAGCCC TCAGTACGTG GAGGCTGCCT GGTACCCTTG GTGGGAGCAG 
CAGGGCTTCT TCAGGCCTGA GTATGGGCGT TCCAGCGTGT CAGCACCAAA CCCCCGGGGC 
ATCTTCATGA TGTGCATCCC ACCCCCCAAC GTGACAGGCT CCCTGCACCT GGGCCATGCG 
CTCACCAACG CCATCCAGGA CTCCCTGACC CGCTGGCACC GCATGCGCGG AGAGACCACC 
CTGTGGAACC CTGGCTGTGA CCACGCGGGC ATTGCCACCC AGGTGGTGGT GGAGAAGAAA 
CTCTGGCGCG AGCAGGGGCT GAGTCGGCAC CAGCTGGGGC GAGAGGCTTT CCTGCAGGAG 
GTCTGGAAGT GGAAGGAGGA GAAAGGTGAC CGGATTTACC ACCAGCTGAA GAAGCTTGGC 
AGCTCCTTGG ACTGGAATCG AGCCTGTTTC ACCATGGATC CTAAACTGTC AGCAGCCGTG 
ACGGAGGCCT TTGTACGGCT CCACGAGGAG GGAATCATTT ACCGCAGCAC CCGCCTTGTC 
AACTGGTCCT GCACCCTCAA CTCTGCCATC TCCGACATCG AGGTGGATAA GAAGGAGCTG 
ACAGGTCGCA CACTGCTCTC CGTGCCTGGC TACAAAGAGA AGGTGGAGTT TGGGGTCCTT 
GTCTCCTTTG CTTACAAGGT CCAAGGCTCA GACAGCAACG ACGAGGTGGT AGTGGCAACC 
ACTCGGATCG AGACGATGCT GGGAGATGTG GCCGTAGCTG TGCATCCCAA AGACCCCAGA 
TACCAGCACC TGAAGGGCAA GAGCGTGATC CACCCATTTG TGTCTCGGAG CCTCCCCATC 
GTCTTTGATG ACTTTGTGGA CATGGAGTTC GGCACAGGTG CGGTGAAGAT CACCCCCGCC 
CACGACCAGA ATGACTATGA GGTTGGGCAG CGGCACGGGC TGGAGGCTGT CAGCATTATG 
GACGCCCGCG GGGCCCTCGT CAACGTGCCC CCACCTTTCC TGGGCCTGCC CAGGTTTGAG 
GCCAGAAAGG CGGTGCTGGC AGCGCTCAAG GAGCAGGGGC TGTTCCGCGG CATTGAGGAC 
AACCCCATGG TGGTGCCACT TTGCAACCGC TCCAAGGACG TGGTGGAGCC TCTGCTGCGG 
CCGCAGTGGT ACGTGCGCTG TGGGGAGATG GCTCAGGCCG CCAGCGCTGC CGTGACACGG 
GGTGACCTCC GCATCCTCCC GGAGGCCCAT CAGCGGACGT GGCACGCCTG GATGGACAAC 
ATCCGGGACT GGTGTATCTC CCGGCAGCTG TGGTGGGGTC ATCGCATCCC AGCCTACTTC 
ATCACTGTCA ATGACCCCGC CGTGCCCCCA GGGGAGGACC CTGACGGGCG GTACTGGGTG 
AGCGGGCGCA CTGAGGCCGA GGCCCGGGAG AAGGCAGCCA AGGAGTTCTG CGTGTCCCCT 
GACAAGATCA GTCTCCAGCA AGATGAAGAT GTACTGGACA CCTGGTTCTC CTCTGGCCTC 
TTCCCCTTCT CCATCCTAGG CTGGCCCAAC CAGTCAGAAG ATCTGAGTGT GTTCTACCCG 
GGGACGCTGC TGGAGACAGG CCATGACATC CTCTTCTTCT GGGTGGCCAG GATGGTCATG 
CTTGGCCTCA AGCTCACTGG CAAGCTGCCC TTCAAAGAGG TCTACCTCCA TGCCATCGTG 
CGGGACGCCC ACGGCCGGAA GATGAGCAAG TCTCTAGGCA ATGTCATTGA CCCCCTGGAC 
GTTATCCATG GGGTCACCCT GCAGGGTCTC CACGACCAGT TACTGAATAG CAACCTGGAT 
CCCAGCGAGG TGGAGAAGGC AAAAGAGGGG CAGAAGGCAG ATTTCCCGAC GGGGATCCCC 
GAGTGTGGCA CCGATGCGCT CCGGTTTGGA CTATGCGCCT ACACGTCCCA GGGCCGTGAC 
ATCAACCTGG ATGTGAACCG GATATTGGGG TACCGCCATT TCTGCAACAA GCTCTGGAAC 
GCCACCAAGT TTGCCCTCCG TGGCCTTGGG AAGGGTTTCG TGCCCTCACC CACGTCTGAG 
CCTGGAGGCC GCGAGAGTCT GGTGGACCGC TGGATCCGCA GCCGGCTGAC CGAAGCTGTG 
AGGCTCAGTA ACCAAGGTTT CCAGGCCTAC GACTTCCCGG CTGTCACCAC CGCCCAGTAC 
AGCTTCTGGC TCTATGAGCT CTGTGATGTC TACCTGGAGT GCCTTAAGCC TGTGCTGAAT 
GGGGTGGACC AGGTAGCAGC CGAGTGCGCC CGCCAGACCC TCTACACCTG CCTGGACGTG 
GGCCTGCGGC TACTCTCACC TTTCATGCCC TTCGTGACTG AGGAGCTGTT CCAGCGGCTG 
CCCCGGCGGA CACCACAAGC TCCCCCTAGC CTCTGCGTCA CTGCCTACCC AGAGCCCTCA 
GAGTGCTCCT GGAAGGACCC TGAGGCAGAA GCTGCCTTCG AGCTGGCCCT GAGCATCACT 
CGAGCCGTGC GCTCCCTGCG TGCCGACTAC AACCTTACCC GGATCCGGCC TGACTGTTTC 
CTGGAAGTGG CTGACGAGGC CACAGGTGCC CTGGCATCAG CGGTGTCGGG CTACGTGCAG 
ACACTGGCCA GCGCGGGGAT TGTGGCCGTC CTGGCGCTGG GGGCTTCTGC ACCCCAGGGC 
TGCGCTGTGG CCCTGGCCTC TGACCGCTGC TCCGTCCACC TGCAGCTGCA GGGGCTAGTA 
GACCCAGCCC GGGAGCTGGG CAAACTGCAG ACCAAGCGTG ACGAGGCGCA GCGGCAGGCC 
CAGCGTCTGC GGGAGCGCCG TGCCGCCTCA GGCTACACTG TCAAGGTGCC CCTCAAAGTC 
CAGGAGGCAG ATGAAGCCAA GCTCCAGCAG ACAGAAGCAG AGCTCAGGAA GGTTGATGAG 
GCCATTGCCC TGTTTCAGAA GATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324426.1
CDS	89..2998
Translation	MPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSNDEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAVSIMDARGALVNVPPPFLGLPRFEARKAVLAALKEQGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRTEAEAREKAAKEFCVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVTLQGLHDQLLNSNLDPSEVEKAKEGQKADFPTGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGRESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTAYPEPSECSWKDPEAEAAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRDEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_015468940.1
Organism	Bos taurus(cattle)
Definition	Bos taurus valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468940.1

ORF Insert Sequence:

ATGCCTGACT CCTACAGCCC TCAGTACGTG GAGGCTGCCT GGTACCCTTG GTGGGAGCAG 
CAGGGCTTCT TCAGGCCTGA GTATGGGCGT TCCAGCGTGT CAGCACCAAA CCCCCGGGGC 
ATCTTCATGA TGTGCATCCC ACCCCCCAAC GTGACAGGCT CCCTGCACCT GGGCCATGCG 
CTCACCAACG CCATCCAGGA CTCCCTGACC CGCTGGCACC GCATGCGCGG AGAGACCACC 
CTGTGGAACC CTGGCTGTGA CCACGCGGGC ATTGCCACCC AGGTGGTGGT GGAGAAGAAA 
CTCTGGCGCG AGCAGGGGCT GAGTCGGCAC CAGCTGGGGC GAGAGGCTTT CCTGCAGGAG 
GTCTGGAAGT GGAAGGAGGA GAAAGGTGAC CGGATTTACC ACCAGCTGAA GAAGCTTGGC 
AGCTCCTTGG ACTGGAATCG AGCCTGTTTC ACCATGGATC CTAAACTGTC AGCAGCCGTG 
ACGGAGGCCT TTGTACGGCT CCACGAGGAG GGAATCATTT ACCGCAGCAC CCGCCTTGTC 
AACTGGTCCT GCACCCTCAA CTCTGCCATC TCCGACATCG AGGTGGATAA GAAGGAGCTG 
ACAGGTCGCA CACTGCTCTC CGTGCCTGGC TACAAAGAGA AGGTGGAGTT TGGGGTCCTT 
GTCTCCTTTG CTTACAAGGT CCAAGGCTCA GACAGCAACG ACGAGGTGGT AGTGGCAACC 
ACTCGGATCG AGACGATGCT GGGAGATGTG GCCGTAGCTG TGCATCCCAA AGACCCCAGA 
TACCAGCACC TGAAGGGCAA GAGCGTGATC CACCCATTTG TGTCTCGGAG CCTCCCCATC 
GTCTTTGATG ACTTTGTGGA CATGGAGTTC GGCACAGGTG CGGTGAAGAT CACCCCCGCC 
CACGACCAGA ATGACTATGA GGTTGGGCAG CGGCACGGGC TGGAGGCTGT CAGCATTATG 
GACGCCCGCG GGGCCCTCGT CAACGTGCCC CCACCTTTCC TGGGCCTGCC CAGGTTTGAG 
GCCAGAAAGG CGGTGCTGGC AGCGCTCAAG GAGCAGGGGC TGTTCCGCGG CATTGAGGAC 
AACCCCATGG TGGTGCCACT TTGCAACCGC TCCAAGGACG TGGTGGAGCC TCTGCTGCGG 
CCGCAGTGGT ACGTGCGCTG TGGGGAGATG GCTCAGGCCG CCAGCGCTGC CGTGACACGG 
GGTGACCTCC GCATCCTCCC GGAGGCCCAT CAGCGGACGT GGCACGCCTG GATGGACAAC 
ATCCGGGACT GGTGTATCTC CCGGCAGCTG TGGTGGGGTC ATCGCATCCC AGCCTACTTC 
ATCACTGTCA ATGACCCCGC CGTGCCCCCA GGGGAGGACC CTGACGGGCG GTACTGGGTG 
AGCGGGCGCA CTGAGGCCGA GGCCCGGGAG AAGGCAGCCA AGGAGTTCTG CGTGTCCCCT 
GACAAGATCA GTCTCCAGCA AGATGAAGAT GTACTGGACA CCTGGTTCTC CTCTGGCCTC 
TTCCCCTTCT CCATCCTAGG CTGGCCCAAC CAGTCAGAAG ATCTGAGTGT GTTCTACCCG 
GGGACGCTGC TGGAGACAGG CCATGACATC CTCTTCTTCT GGGTGGCCAG GATGGTCATG 
CTTGGCCTCA AGCTCACTGG CAAGCTGCCC TTCAAAGAGG TCTACCTCCA TGCCATCGTG 
CGGGACGCCC ACGGCCGGAA GATGAGCAAG TCTCTAGGCA ATGTCATTGA CCCCCTGGAC 
GTTATCCATG GGGTCACCCT GCAGGGTCTC CACGACCAGT TACTGAATAG CAACCTGGAT 
CCCAGCGAGG TGGAGAAGGC AAAAGAGGGG CAGAAGGCAG ATTTCCCGAC GGGGATCCCC 
GAGTGTGGCA CCGATGCGCT CCGGTTTGGA CTATGCGCCT ACACGTCCCA GGGCCGTGAC 
ATCAACCTGG ATGTGAACCG GATATTGGGG TACCGCCATT TCTGCAACAA GCTCTGGAAC 
GCCACCAAGT TTGCCCTCCG TGGCCTTGGG AAGGGTTTCG TGCCCTCACC CACGTCTGAG 
CCTGGAGGCC GCGAGAGTCT GGTGGACCGC TGGATCCGCA GCCGGCTGAC CGAAGCTGTG 
AGGCTCAGTA ACCAAGGTTT CCAGGCCTAC GACTTCCCGG CTGTCACCAC CGCCCAGTAC 
AGCTTCTGGC TCTATGAGCT CTGTGATGTC TACCTGGAGT GCCTTAAGCC TGTGCTGAAT 
GGGGTGGACC AGGTAGCAGC CGAGTGCGCC CGCCAGACCC TCTACACCTG CCTGGACGTG 
GGCCTGCGGC TACTCTCACC TTTCATGCCC TTCGTGACTG AGGAGCTGTT CCAGCGGCTG 
CCCCGGCGGA CACCACAAGC TCCCCCTAGC CTCTGCGTCA CTGCCTACCC AGAGCCCTCA 
GAGTGCTCCT GGAAGGACCC TGAGGCAGAA GCTGCCTTCG AGCTGGCCCT GAGCATCACT 
CGAGCCGTGC GCTCCCTGCG TGCCGACTAC AACCTTACCC GGATCCGGCC TGACTGTTTC 
CTGGAAGTGG CTGACGAGGC CACAGGTGCC CTGGCATCAG CGGTGTCGGG CTACGTGCAG 
ACACTGGCCA GCGCGGGGAT TGTGGCCGTC CTGGCGCTGG GGGCTTCTGC ACCCCAGGGC 
TGCGCTGTGG CCCTGGCCTC TGACCGCTGC TCCGTCCACC TGCAGCTGCA GGGGCTAGTA 
GACCCAGCCC GGGAGCTGGG CAAACTGCAG ACCAAGCGTG ACGAGGCGCA GCGGCAGGCC 
CAGCGTCTGC GGGAGCGCCG TGCCGCCTCA GGCTACACTG TCAAGGTGCC CCTCAAAGTC 
CAGGAGGCAG ATGAAGCCAA GCTCCAGCAG ACAGAAGCAG AGCTCAGGAA GGTTGATGAG 
GCCATTGCCC TGTTTCAGAA GATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_024984080.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839848.1 Bos taurus valyl-tRNA synthetase (VARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229798
Clone ID Related Accession (Same CDS sequence)	XM_024984080.1
Accession Version	XM_024984080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	valine--tRNA ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCG GTACCGGGCG CCGTCTGCAT CGGGTAGCGC TCTCCCAGCC GCTTCCCCGG 
CTGTCCGCTC GGCGCCGCCC TCCCTCCCGG CCGAGGACCC GCGCGGGGTG TCCGCGGCGG 
CGCCTGACGG GGTCGCGGTT CCCGGGGGCG TCCTCCTCCC GCCACCCCCG TGCCCACGCC 
CCGCGCCATT CCTCAGGAGC GCGATCTGCA GCGGGCGGCG TGACCGCCCC CAGGCCAGGG 
TGCAGCAGGA CCGCGCCCGC ACACGCAGGC GGGGCACGGT CGCCCGCGCC AGGACCCGCG 
GGCCCGGAAA CGCTCGCAGT CACAAAGGGG GGGACACGTG GGCGCCGACG GCCCGGGCGG 
CTGTCGCAGG GAGCTAGGGT CGCCCAGAGA ACCGCCCGCC GTCCCTGGTC GCCGGATTCC 
TCGGCTGCAG CCGCCAGGCC AGTCCAGCTT CGGCCGGCGC CCGGCTATGA GGTGGATGTC 
CGGCTCAGTG TGACCACCTC CCTCCAGGTT TTTCTACCGC GTTCGGACAT CCCGCGTAAC 
CATGTCCATT CTGCTGCACT GGCGGCCCTG GGCAGAGCCC TGAGTCCCTT GGAAGAATGG 
CTTCGACTGC ATACCTACCT GGCCGGGGAG GCCCCAACTC TGGCTGACCT GGCAGCTGTC 
ACAGCCTTGC TGCTGCCTTT CCGTTATGTC CTGGACCCCT CTGCCCGCCG GATCTGGGGT 
AATGTGACTC GCTGGTTTAT CACATGTGTT CAGCAGCCAG AATTCCGGGC CGTACTGGGA 
GAAGTGGTTC TGTATTCAGG GGCCAGGTCT CTCTCCCAAC AGCCAGGCTC TGAGGTCCCT 
GCACCCCCCA AAACGGCTGC TCAACTCAAG AAAGAGGCAA AGAAACGGGA AAAACTAGAG 
AAATTCCAGC AGAAGCAGAA AGTCCAACAG CAGCAGCCAC CCCCTGGGGA GCAGAAGAAA 
CCAAAACCAG AGAAGAGGGA GAAACGGGAT CCTGGGGTCA TTACCTACGA TCTCCCGACC 
CCACCTGGGG AAAAGAAAGA TGTCAGTGGC ACCATGCCTG ACTCCTACAG CCCTCAGTAC 
GTGGAGGCTG CCTGGTACCC TTGGTGGGAG CAGCAGGGCT TCTTCAGGCC TGAGTATGGG 
CGTTCCAGCG TGTCAGCACC AAACCCCCGG GGCATCTTCA TGATGTGCAT CCCACCCCCC 
AACGTGACAG GCTCCCTGCA CCTGGGCCAT GCGCTCACCA ACGCCATCCA GGACTCCCTG 
ACCCGCTGGC ACCGCATGCG CGGAGAGACC ACCCTGTGGA ACCCTGGCTG TGACCACGCG 
GGCATTGCCA CCCAGGTGGT GGTGGAGAAG AAACTCTGGC GCGAGCAGGG GCTGAGTCGG 
CACCAGCTGG GGCGAGAGGC TTTCCTGCAG GAGGTCTGGA AGTGGAAGGA GGAGAAAGGT 
GACCGGATTT ACCACCAGCT GAAGAAGCTT GGCAGCTCCT TGGACTGGAA TCGAGCCTGT 
TTCACCATGG ATCCTAAACT GTCAGCAGCC GTGACGGAGG CCTTTGTACG GCTCCACGAG 
GAGGGAATCA TTTACCGCAG CACCCGCCTT GTCAACTGGT CCTGCACCCT CAACTCTGCC 
ATCTCCGACA TCGAGGTGGA TAAGAAGGAG CTGACAGGTC GCACACTGCT CTCCGTGCCT 
GGCTACAAAG AGAAGGTGGA GTTTGGGGTC CTTGTCTCCT TTGCTTACAA GGTCCAAGGC 
TCAGACAGCA ACGACGAGGT GGTAGTGGCA ACCACTCGGA TCGAGACGAT GCTGGGAGAT 
GTGGCCGTAG CTGTGCATCC CAAAGACCCC AGATACCAGC ACCTGAAGGG CAAGAGCGTG 
ATCCACCCAT TTGTGTCTCG GAGCCTCCCC ATCGTCTTTG ATGACTTTGT GGACATGGAG 
TTCGGCACAG GTGCGGTGAA GATCACCCCC GCCCACGACC AGAATGACTA TGAGGTTGGG 
CAGCGGCACG GGCTGGAGGC TGTCAGCATT ATGGACGCCC GCGGGGCCCT CGTCAACGTG 
CCCCCACCTT TCCTGGGCCT GCCCAGGTTT GAGGCCAGAA AGGCGGTGCT GGCAGCGCTC 
AAGGAGCAGG GGCTGTTCCG CGGCATTGAG GACAACCCCA TGGTGGTGCC ACTTTGCAAC 
CGCTCCAAGG ACGTGGTGGA GCCTCTGCTG CGGCCGCAGT GGTACGTGCG CTGTGGGGAG 
ATGGCTCAGG CCGCCAGCGC TGCCGTGACA CGGGGTGACC TCCGCATCCT CCCGGAGGCC 
CATCAGCGGA CGTGGCACGC CTGGATGGAC AACATCCGGG ACTGGTGTAT CTCCCGGCAG 
CTGTGGTGGG GTCATCGCAT CCCAGCCTAC TTCATCACTG TCAATGACCC CGCCGTGCCC 
CCAGGGGAGG ACCCTGACGG GCGGTACTGG GTGAGCGGGC GCACTGAGGC CGAGGCCCGG 
GAGAAGGCAG CCAAGGAGTT CTGCGTGTCC CCTGACAAGA TCAGTCTCCA GCAAGATGAA 
GATGTACTGG ACACCTGGTT CTCCTCTGGC CTCTTCCCCT TCTCCATCCT AGGCTGGCCC 
AACCAGTCAG AAGATCTGAG TGTGTTCTAC CCGGGGACGC TGCTGGAGAC AGGCCATGAC 
ATCCTCTTCT TCTGGGTGGC CAGGATGGTC ATGCTTGGCC TCAAGCTCAC TGGCAAGCTG 
CCCTTCAAAG AGGTCTACCT CCATGCCATC GTGCGGGACG CCCACGGCCG GAAGATGAGC 
AAGTCTCTAG GCAATGTCAT TGACCCCCTG GACGTTATCC ATGGGGTCAC CCTGCAGGGT 
CTCCACGACC AGTTACTGAA TAGCAACCTG GATCCCAGCG AGGTGGAGAA GGCAAAAGAG 
GGGCAGAAGG CAGATTTCCC GACGGGGATC CCCGAGTGTG GCACCGATGC GCTCCGGTTT 
GGACTATGCG CCTACACGTC CCAGGGCCGT GACATCAACC TGGATGTGAA CCGGATATTG 
GGGTACCGCC ATTTCTGCAA CAAGCTCTGG AACGCCACCA AGTTTGCCCT CCGTGGCCTT 
GGGAAGGGTT TCGTGCCCTC ACCCACGTCT GAGCCTGGAG GCCGCGAGAG TCTGGTGGAC 
CGCTGGATCC GCAGCCGGCT GACCGAAGCT GTGAGGCTCA GTAACCAAGG TTTCCAGGCC 
TACGACTTCC CGGCTGTCAC CACCGCCCAG TACAGCTTCT GGCTCTATGA GCTCTGTGAT 
GTCTACCTGG AGTGCCTTAA GCCTGTGCTG AATGGGGTGG ACCAGGTAGC AGCCGAGTGC 
GCCCGCCAGA CCCTCTACAC CTGCCTGGAC GTGGGCCTGC GGCTACTCTC ACCTTTCATG 
CCCTTCGTGA CTGAGGAGCT GTTCCAGCGG CTGCCCCGGC GGACACCACA AGCTCCCCCT 
AGCCTCTGCG TCACTGCCTA CCCAGAGCCC TCAGAGTGCT CCTGGAAGGA CCCTGAGGCA 
GAAGCTGCCT TCGAGCTGGC CCTGAGCATC ACTCGAGCCG TGCGCTCCCT GCGTGCCGAC 
TACAACCTTA CCCGGATCCG GCCTGACTGT TTCCTGGAAG TGGCTGACGA GGCCACAGGT 
GCCCTGGCAT CAGCGGTGTC GGGCTACGTG CAGACACTGG CCAGCGCGGG GATTGTGGCC 
GTCCTGGCGC TGGGGGCTTC TGCACCCCAG GGCTGCGCTG TGGCCCTGGC CTCTGACCGC 
TGCTCCGTCC ACCTGCAGCT GCAGGGGCTA GTAGACCCAG CCCGGGAGCT GGGCAAACTG 
CAGACCAAGC GTGACGAGGC GCAGCGGCAG GCCCAGCGTC TGCGGGAGCG CCGTGCCGCC 
TCAGGCTACA CTGTCAAGGT GCCCCTCAAA GTCCAGGAGG CAGATGAAGC CAAGCTCCAG 
CAGACAGAAG CAGAGCTCAG GAAGGTTGAT GAGGCCATTG CCCTGTTTCA GAAGATGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839848.1
CDS	1101..5063
Translation	MDPGTGRRLHRVALSQPLPRLSARRRPPSRPRTRAGCPRRRLTGSRFPGASSSRHPRAHAPRHSSGARSAAGGVTAPRPGCSRTAPAHAGGARSPAPGPAGPETLAVTKGGTRGRRRPGRLSQGARVAQRTARRPWSPDSSAAAARPVQLRPAPGYEVDVRLSVTTSLQVFLPRSDIPRNHVHSAALAALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFITCVQQPEFRAVLGEVVLYSGARSLSQQPGSEVPAPPKTAAQLKKEAKKREKLEKFQQKQKVQQQQPPPGEQKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSNDEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAVSIMDARGALVNVPPPFLGLPRFEARKAVLAALKEQGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRTEAEAREKAAKEFCVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVTLQGLHDQLLNSNLDPSEVEKAKEGQKADFPTGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGRESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTAYPEPSECSWKDPEAEAAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRDEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_024984080.1
Organism	Bos taurus(cattle)
Definition	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984080.1

ORF Insert Sequence:

ATGGACCCCG GTACCGGGCG CCGTCTGCAT CGGGTAGCGC TCTCCCAGCC GCTTCCCCGG 
CTGTCCGCTC GGCGCCGCCC TCCCTCCCGG CCGAGGACCC GCGCGGGGTG TCCGCGGCGG 
CGCCTGACGG GGTCGCGGTT CCCGGGGGCG TCCTCCTCCC GCCACCCCCG TGCCCACGCC 
CCGCGCCATT CCTCAGGAGC GCGATCTGCA GCGGGCGGCG TGACCGCCCC CAGGCCAGGG 
TGCAGCAGGA CCGCGCCCGC ACACGCAGGC GGGGCACGGT CGCCCGCGCC AGGACCCGCG 
GGCCCGGAAA CGCTCGCAGT CACAAAGGGG GGGACACGTG GGCGCCGACG GCCCGGGCGG 
CTGTCGCAGG GAGCTAGGGT CGCCCAGAGA ACCGCCCGCC GTCCCTGGTC GCCGGATTCC 
TCGGCTGCAG CCGCCAGGCC AGTCCAGCTT CGGCCGGCGC CCGGCTATGA GGTGGATGTC 
CGGCTCAGTG TGACCACCTC CCTCCAGGTT TTTCTACCGC GTTCGGACAT CCCGCGTAAC 
CATGTCCATT CTGCTGCACT GGCGGCCCTG GGCAGAGCCC TGAGTCCCTT GGAAGAATGG 
CTTCGACTGC ATACCTACCT GGCCGGGGAG GCCCCAACTC TGGCTGACCT GGCAGCTGTC 
ACAGCCTTGC TGCTGCCTTT CCGTTATGTC CTGGACCCCT CTGCCCGCCG GATCTGGGGT 
AATGTGACTC GCTGGTTTAT CACATGTGTT CAGCAGCCAG AATTCCGGGC CGTACTGGGA 
GAAGTGGTTC TGTATTCAGG GGCCAGGTCT CTCTCCCAAC AGCCAGGCTC TGAGGTCCCT 
GCACCCCCCA AAACGGCTGC TCAACTCAAG AAAGAGGCAA AGAAACGGGA AAAACTAGAG 
AAATTCCAGC AGAAGCAGAA AGTCCAACAG CAGCAGCCAC CCCCTGGGGA GCAGAAGAAA 
CCAAAACCAG AGAAGAGGGA GAAACGGGAT CCTGGGGTCA TTACCTACGA TCTCCCGACC 
CCACCTGGGG AAAAGAAAGA TGTCAGTGGC ACCATGCCTG ACTCCTACAG CCCTCAGTAC 
GTGGAGGCTG CCTGGTACCC TTGGTGGGAG CAGCAGGGCT TCTTCAGGCC TGAGTATGGG 
CGTTCCAGCG TGTCAGCACC AAACCCCCGG GGCATCTTCA TGATGTGCAT CCCACCCCCC 
AACGTGACAG GCTCCCTGCA CCTGGGCCAT GCGCTCACCA ACGCCATCCA GGACTCCCTG 
ACCCGCTGGC ACCGCATGCG CGGAGAGACC ACCCTGTGGA ACCCTGGCTG TGACCACGCG 
GGCATTGCCA CCCAGGTGGT GGTGGAGAAG AAACTCTGGC GCGAGCAGGG GCTGAGTCGG 
CACCAGCTGG GGCGAGAGGC TTTCCTGCAG GAGGTCTGGA AGTGGAAGGA GGAGAAAGGT 
GACCGGATTT ACCACCAGCT GAAGAAGCTT GGCAGCTCCT TGGACTGGAA TCGAGCCTGT 
TTCACCATGG ATCCTAAACT GTCAGCAGCC GTGACGGAGG CCTTTGTACG GCTCCACGAG 
GAGGGAATCA TTTACCGCAG CACCCGCCTT GTCAACTGGT CCTGCACCCT CAACTCTGCC 
ATCTCCGACA TCGAGGTGGA TAAGAAGGAG CTGACAGGTC GCACACTGCT CTCCGTGCCT 
GGCTACAAAG AGAAGGTGGA GTTTGGGGTC CTTGTCTCCT TTGCTTACAA GGTCCAAGGC 
TCAGACAGCA ACGACGAGGT GGTAGTGGCA ACCACTCGGA TCGAGACGAT GCTGGGAGAT 
GTGGCCGTAG CTGTGCATCC CAAAGACCCC AGATACCAGC ACCTGAAGGG CAAGAGCGTG 
ATCCACCCAT TTGTGTCTCG GAGCCTCCCC ATCGTCTTTG ATGACTTTGT GGACATGGAG 
TTCGGCACAG GTGCGGTGAA GATCACCCCC GCCCACGACC AGAATGACTA TGAGGTTGGG 
CAGCGGCACG GGCTGGAGGC TGTCAGCATT ATGGACGCCC GCGGGGCCCT CGTCAACGTG 
CCCCCACCTT TCCTGGGCCT GCCCAGGTTT GAGGCCAGAA AGGCGGTGCT GGCAGCGCTC 
AAGGAGCAGG GGCTGTTCCG CGGCATTGAG GACAACCCCA TGGTGGTGCC ACTTTGCAAC 
CGCTCCAAGG ACGTGGTGGA GCCTCTGCTG CGGCCGCAGT GGTACGTGCG CTGTGGGGAG 
ATGGCTCAGG CCGCCAGCGC TGCCGTGACA CGGGGTGACC TCCGCATCCT CCCGGAGGCC 
CATCAGCGGA CGTGGCACGC CTGGATGGAC AACATCCGGG ACTGGTGTAT CTCCCGGCAG 
CTGTGGTGGG GTCATCGCAT CCCAGCCTAC TTCATCACTG TCAATGACCC CGCCGTGCCC 
CCAGGGGAGG ACCCTGACGG GCGGTACTGG GTGAGCGGGC GCACTGAGGC CGAGGCCCGG 
GAGAAGGCAG CCAAGGAGTT CTGCGTGTCC CCTGACAAGA TCAGTCTCCA GCAAGATGAA 
GATGTACTGG ACACCTGGTT CTCCTCTGGC CTCTTCCCCT TCTCCATCCT AGGCTGGCCC 
AACCAGTCAG AAGATCTGAG TGTGTTCTAC CCGGGGACGC TGCTGGAGAC AGGCCATGAC 
ATCCTCTTCT TCTGGGTGGC CAGGATGGTC ATGCTTGGCC TCAAGCTCAC TGGCAAGCTG 
CCCTTCAAAG AGGTCTACCT CCATGCCATC GTGCGGGACG CCCACGGCCG GAAGATGAGC 
AAGTCTCTAG GCAATGTCAT TGACCCCCTG GACGTTATCC ATGGGGTCAC CCTGCAGGGT 
CTCCACGACC AGTTACTGAA TAGCAACCTG GATCCCAGCG AGGTGGAGAA GGCAAAAGAG 
GGGCAGAAGG CAGATTTCCC GACGGGGATC CCCGAGTGTG GCACCGATGC GCTCCGGTTT 
GGACTATGCG CCTACACGTC CCAGGGCCGT GACATCAACC TGGATGTGAA CCGGATATTG 
GGGTACCGCC ATTTCTGCAA CAAGCTCTGG AACGCCACCA AGTTTGCCCT CCGTGGCCTT 
GGGAAGGGTT TCGTGCCCTC ACCCACGTCT GAGCCTGGAG GCCGCGAGAG TCTGGTGGAC 
CGCTGGATCC GCAGCCGGCT GACCGAAGCT GTGAGGCTCA GTAACCAAGG TTTCCAGGCC 
TACGACTTCC CGGCTGTCAC CACCGCCCAG TACAGCTTCT GGCTCTATGA GCTCTGTGAT 
GTCTACCTGG AGTGCCTTAA GCCTGTGCTG AATGGGGTGG ACCAGGTAGC AGCCGAGTGC 
GCCCGCCAGA CCCTCTACAC CTGCCTGGAC GTGGGCCTGC GGCTACTCTC ACCTTTCATG 
CCCTTCGTGA CTGAGGAGCT GTTCCAGCGG CTGCCCCGGC GGACACCACA AGCTCCCCCT 
AGCCTCTGCG TCACTGCCTA CCCAGAGCCC TCAGAGTGCT CCTGGAAGGA CCCTGAGGCA 
GAAGCTGCCT TCGAGCTGGC CCTGAGCATC ACTCGAGCCG TGCGCTCCCT GCGTGCCGAC 
TACAACCTTA CCCGGATCCG GCCTGACTGT TTCCTGGAAG TGGCTGACGA GGCCACAGGT 
GCCCTGGCAT CAGCGGTGTC GGGCTACGTG CAGACACTGG CCAGCGCGGG GATTGTGGCC 
GTCCTGGCGC TGGGGGCTTC TGCACCCCAG GGCTGCGCTG TGGCCCTGGC CTCTGACCGC 
TGCTCCGTCC ACCTGCAGCT GCAGGGGCTA GTAGACCCAG CCCGGGAGCT GGGCAAACTG 
CAGACCAAGC GTGACGAGGC GCAGCGGCAG GCCCAGCGTC TGCGGGAGCG CCGTGCCGCC 
TCAGGCTACA CTGTCAAGGT GCCCCTCAAA GTCCAGGAGG CAGATGAAGC CAAGCTCCAG 
CAGACAGAAG CAGAGCTCAG GAAGGTTGAT GAGGCCATTG CCCTGTTTCA GAAGATGCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_024984081.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839849.1 Bos taurus valyl-tRNA synthetase (VARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229799
Clone ID Related Accession (Same CDS sequence)	XM_024984081.1
Accession Version	XM_024984081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	valine--tRNA ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCG GTACCGGGCG CCGTCTGCAT CGGGTAGCGC TCTCCCAGCC GCTTCCCCGG 
CTGTCCGCTC GGCGCCGCCC TCCCTCCCGG CCGAGGACCC GCGCGGGGTG TCCGCGGCGG 
CGCCTGACGG GGTCGCGGTT CCCGGGGGCG TCCTCCTCCC GCCACCCCCG TGCCCACGCC 
CCGCGCCATT CCTCAGGAGC GCGATCTGCA GCGGGCGGCG TGACCGCCCC CAGGCCAGGG 
TGCAGCAGGA CCGCGCCCGC ACACGCAGGC GGGGCACGGT CGCCCGCGCC AGGACCCGCG 
GGCCCGGAAA CGCTCGCAGT CACAAAGGGG GGGACACGTG GGCGCCGACG GCCCGGGCGG 
CTGTCGCAGG GAGCTAGGGT CGCCCAGAGA ACCGCCCGCC GTCCCTGGTC GCCGGATTCC 
TCGGCTGCAG CCGCCAGGCC AGTCCAGCTT CGGCCGGCGC CCGGCTATGA GGTGGATGTC 
CGGCTCAGTG TGACCACCTC CCTCCAGGTT TTTCTACCGC GTTCGGACAT CCCGCGTAAC 
CATGTCCATT CTGCTGCACT GGCGGCCCTG GGCAGAGCCC TGAGTCCCTT GGAAGAATGG 
CTTCGACTGC ATACCTACCT GGCCGGGGAG GCCCCAACTC TGGCTGACCT GGCAGCTGTC 
ACAGCCTTGC TGCTGCCTTT CCGTTATGTC CTGGACCCCT CTGCCCGCCG GATCTGGGGT 
AATGTGACTC GCTGGTTTAT CACATGTGTT CAGCAGCCAG AATTCCGGGC CGTACTGGGA 
GAAGTGGTTC TGTATTCAGG GGCCAGGTCT CTCTCCCAAC AGCCAGGCTC TGAGGTCCCT 
GCACCCCCCA AAACGGCTGC TCAACTCAAG AAAGAGGCAA AGAAACGGGA AAAACTAGAG 
AAATTCCAGC AGAAGCAGAA AGTCCAACAG CAGCAGCCAC CCCCTGGGGA GAAGAAACCA 
AAACCAGAGA AGAGGGAGAA ACGGGATCCT GGGGTCATTA CCTACGATCT CCCGACCCCA 
CCTGGGGAAA AGAAAGATGT CAGTGGCACC ATGCCTGACT CCTACAGCCC TCAGTACGTG 
GAGGCTGCCT GGTACCCTTG GTGGGAGCAG CAGGGCTTCT TCAGGCCTGA GTATGGGCGT 
TCCAGCGTGT CAGCACCAAA CCCCCGGGGC ATCTTCATGA TGTGCATCCC ACCCCCCAAC 
GTGACAGGCT CCCTGCACCT GGGCCATGCG CTCACCAACG CCATCCAGGA CTCCCTGACC 
CGCTGGCACC GCATGCGCGG AGAGACCACC CTGTGGAACC CTGGCTGTGA CCACGCGGGC 
ATTGCCACCC AGGTGGTGGT GGAGAAGAAA CTCTGGCGCG AGCAGGGGCT GAGTCGGCAC 
CAGCTGGGGC GAGAGGCTTT CCTGCAGGAG GTCTGGAAGT GGAAGGAGGA GAAAGGTGAC 
CGGATTTACC ACCAGCTGAA GAAGCTTGGC AGCTCCTTGG ACTGGAATCG AGCCTGTTTC 
ACCATGGATC CTAAACTGTC AGCAGCCGTG ACGGAGGCCT TTGTACGGCT CCACGAGGAG 
GGAATCATTT ACCGCAGCAC CCGCCTTGTC AACTGGTCCT GCACCCTCAA CTCTGCCATC 
TCCGACATCG AGGTGGATAA GAAGGAGCTG ACAGGTCGCA CACTGCTCTC CGTGCCTGGC 
TACAAAGAGA AGGTGGAGTT TGGGGTCCTT GTCTCCTTTG CTTACAAGGT CCAAGGCTCA 
GACAGCAACG ACGAGGTGGT AGTGGCAACC ACTCGGATCG AGACGATGCT GGGAGATGTG 
GCCGTAGCTG TGCATCCCAA AGACCCCAGA TACCAGCACC TGAAGGGCAA GAGCGTGATC 
CACCCATTTG TGTCTCGGAG CCTCCCCATC GTCTTTGATG ACTTTGTGGA CATGGAGTTC 
GGCACAGGTG CGGTGAAGAT CACCCCCGCC CACGACCAGA ATGACTATGA GGTTGGGCAG 
CGGCACGGGC TGGAGGCTGT CAGCATTATG GACGCCCGCG GGGCCCTCGT CAACGTGCCC 
CCACCTTTCC TGGGCCTGCC CAGGTTTGAG GCCAGAAAGG CGGTGCTGGC AGCGCTCAAG 
GAGCAGGGGC TGTTCCGCGG CATTGAGGAC AACCCCATGG TGGTGCCACT TTGCAACCGC 
TCCAAGGACG TGGTGGAGCC TCTGCTGCGG CCGCAGTGGT ACGTGCGCTG TGGGGAGATG 
GCTCAGGCCG CCAGCGCTGC CGTGACACGG GGTGACCTCC GCATCCTCCC GGAGGCCCAT 
CAGCGGACGT GGCACGCCTG GATGGACAAC ATCCGGGACT GGTGTATCTC CCGGCAGCTG 
TGGTGGGGTC ATCGCATCCC AGCCTACTTC ATCACTGTCA ATGACCCCGC CGTGCCCCCA 
GGGGAGGACC CTGACGGGCG GTACTGGGTG AGCGGGCGCA CTGAGGCCGA GGCCCGGGAG 
AAGGCAGCCA AGGAGTTCTG CGTGTCCCCT GACAAGATCA GTCTCCAGCA AGATGAAGAT 
GTACTGGACA CCTGGTTCTC CTCTGGCCTC TTCCCCTTCT CCATCCTAGG CTGGCCCAAC 
CAGTCAGAAG ATCTGAGTGT GTTCTACCCG GGGACGCTGC TGGAGACAGG CCATGACATC 
CTCTTCTTCT GGGTGGCCAG GATGGTCATG CTTGGCCTCA AGCTCACTGG CAAGCTGCCC 
TTCAAAGAGG TCTACCTCCA TGCCATCGTG CGGGACGCCC ACGGCCGGAA GATGAGCAAG 
TCTCTAGGCA ATGTCATTGA CCCCCTGGAC GTTATCCATG GGGTCACCCT GCAGGGTCTC 
CACGACCAGT TACTGAATAG CAACCTGGAT CCCAGCGAGG TGGAGAAGGC AAAAGAGGGG 
CAGAAGGCAG ATTTCCCGAC GGGGATCCCC GAGTGTGGCA CCGATGCGCT CCGGTTTGGA 
CTATGCGCCT ACACGTCCCA GGGCCGTGAC ATCAACCTGG ATGTGAACCG GATATTGGGG 
TACCGCCATT TCTGCAACAA GCTCTGGAAC GCCACCAAGT TTGCCCTCCG TGGCCTTGGG 
AAGGGTTTCG TGCCCTCACC CACGTCTGAG CCTGGAGGCC GCGAGAGTCT GGTGGACCGC 
TGGATCCGCA GCCGGCTGAC CGAAGCTGTG AGGCTCAGTA ACCAAGGTTT CCAGGCCTAC 
GACTTCCCGG CTGTCACCAC CGCCCAGTAC AGCTTCTGGC TCTATGAGCT CTGTGATGTC 
TACCTGGAGT GCCTTAAGCC TGTGCTGAAT GGGGTGGACC AGGTAGCAGC CGAGTGCGCC 
CGCCAGACCC TCTACACCTG CCTGGACGTG GGCCTGCGGC TACTCTCACC TTTCATGCCC 
TTCGTGACTG AGGAGCTGTT CCAGCGGCTG CCCCGGCGGA CACCACAAGC TCCCCCTAGC 
CTCTGCGTCA CTGCCTACCC AGAGCCCTCA GAGTGCTCCT GGAAGGACCC TGAGGCAGAA 
GCTGCCTTCG AGCTGGCCCT GAGCATCACT CGAGCCGTGC GCTCCCTGCG TGCCGACTAC 
AACCTTACCC GGATCCGGCC TGACTGTTTC CTGGAAGTGG CTGACGAGGC CACAGGTGCC 
CTGGCATCAG CGGTGTCGGG CTACGTGCAG ACACTGGCCA GCGCGGGGAT TGTGGCCGTC 
CTGGCGCTGG GGGCTTCTGC ACCCCAGGGC TGCGCTGTGG CCCTGGCCTC TGACCGCTGC 
TCCGTCCACC TGCAGCTGCA GGGGCTAGTA GACCCAGCCC GGGAGCTGGG CAAACTGCAG 
ACCAAGCGTG ACGAGGCGCA GCGGCAGGCC CAGCGTCTGC GGGAGCGCCG TGCCGCCTCA 
GGCTACACTG TCAAGGTGCC CCTCAAAGTC CAGGAGGCAG ATGAAGCCAA GCTCCAGCAG 
ACAGAAGCAG AGCTCAGGAA GGTTGATGAG GCCATTGCCC TGTTTCAGAA GATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839849.1
CDS	1101..5060
Translation	MDPGTGRRLHRVALSQPLPRLSARRRPPSRPRTRAGCPRRRLTGSRFPGASSSRHPRAHAPRHSSGARSAAGGVTAPRPGCSRTAPAHAGGARSPAPGPAGPETLAVTKGGTRGRRRPGRLSQGARVAQRTARRPWSPDSSAAAARPVQLRPAPGYEVDVRLSVTTSLQVFLPRSDIPRNHVHSAALAALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFITCVQQPEFRAVLGEVVLYSGARSLSQQPGSEVPAPPKTAAQLKKEAKKREKLEKFQQKQKVQQQQPPPGEKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSNDEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAVSIMDARGALVNVPPPFLGLPRFEARKAVLAALKEQGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRTEAEAREKAAKEFCVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVTLQGLHDQLLNSNLDPSEVEKAKEGQKADFPTGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGRESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTAYPEPSECSWKDPEAEAAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRDEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_024984081.1
Organism	Bos taurus(cattle)
Definition	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984081.1

ORF Insert Sequence:

ATGGACCCCG GTACCGGGCG CCGTCTGCAT CGGGTAGCGC TCTCCCAGCC GCTTCCCCGG 
CTGTCCGCTC GGCGCCGCCC TCCCTCCCGG CCGAGGACCC GCGCGGGGTG TCCGCGGCGG 
CGCCTGACGG GGTCGCGGTT CCCGGGGGCG TCCTCCTCCC GCCACCCCCG TGCCCACGCC 
CCGCGCCATT CCTCAGGAGC GCGATCTGCA GCGGGCGGCG TGACCGCCCC CAGGCCAGGG 
TGCAGCAGGA CCGCGCCCGC ACACGCAGGC GGGGCACGGT CGCCCGCGCC AGGACCCGCG 
GGCCCGGAAA CGCTCGCAGT CACAAAGGGG GGGACACGTG GGCGCCGACG GCCCGGGCGG 
CTGTCGCAGG GAGCTAGGGT CGCCCAGAGA ACCGCCCGCC GTCCCTGGTC GCCGGATTCC 
TCGGCTGCAG CCGCCAGGCC AGTCCAGCTT CGGCCGGCGC CCGGCTATGA GGTGGATGTC 
CGGCTCAGTG TGACCACCTC CCTCCAGGTT TTTCTACCGC GTTCGGACAT CCCGCGTAAC 
CATGTCCATT CTGCTGCACT GGCGGCCCTG GGCAGAGCCC TGAGTCCCTT GGAAGAATGG 
CTTCGACTGC ATACCTACCT GGCCGGGGAG GCCCCAACTC TGGCTGACCT GGCAGCTGTC 
ACAGCCTTGC TGCTGCCTTT CCGTTATGTC CTGGACCCCT CTGCCCGCCG GATCTGGGGT 
AATGTGACTC GCTGGTTTAT CACATGTGTT CAGCAGCCAG AATTCCGGGC CGTACTGGGA 
GAAGTGGTTC TGTATTCAGG GGCCAGGTCT CTCTCCCAAC AGCCAGGCTC TGAGGTCCCT 
GCACCCCCCA AAACGGCTGC TCAACTCAAG AAAGAGGCAA AGAAACGGGA AAAACTAGAG 
AAATTCCAGC AGAAGCAGAA AGTCCAACAG CAGCAGCCAC CCCCTGGGGA GAAGAAACCA 
AAACCAGAGA AGAGGGAGAA ACGGGATCCT GGGGTCATTA CCTACGATCT CCCGACCCCA 
CCTGGGGAAA AGAAAGATGT CAGTGGCACC ATGCCTGACT CCTACAGCCC TCAGTACGTG 
GAGGCTGCCT GGTACCCTTG GTGGGAGCAG CAGGGCTTCT TCAGGCCTGA GTATGGGCGT 
TCCAGCGTGT CAGCACCAAA CCCCCGGGGC ATCTTCATGA TGTGCATCCC ACCCCCCAAC 
GTGACAGGCT CCCTGCACCT GGGCCATGCG CTCACCAACG CCATCCAGGA CTCCCTGACC 
CGCTGGCACC GCATGCGCGG AGAGACCACC CTGTGGAACC CTGGCTGTGA CCACGCGGGC 
ATTGCCACCC AGGTGGTGGT GGAGAAGAAA CTCTGGCGCG AGCAGGGGCT GAGTCGGCAC 
CAGCTGGGGC GAGAGGCTTT CCTGCAGGAG GTCTGGAAGT GGAAGGAGGA GAAAGGTGAC 
CGGATTTACC ACCAGCTGAA GAAGCTTGGC AGCTCCTTGG ACTGGAATCG AGCCTGTTTC 
ACCATGGATC CTAAACTGTC AGCAGCCGTG ACGGAGGCCT TTGTACGGCT CCACGAGGAG 
GGAATCATTT ACCGCAGCAC CCGCCTTGTC AACTGGTCCT GCACCCTCAA CTCTGCCATC 
TCCGACATCG AGGTGGATAA GAAGGAGCTG ACAGGTCGCA CACTGCTCTC CGTGCCTGGC 
TACAAAGAGA AGGTGGAGTT TGGGGTCCTT GTCTCCTTTG CTTACAAGGT CCAAGGCTCA 
GACAGCAACG ACGAGGTGGT AGTGGCAACC ACTCGGATCG AGACGATGCT GGGAGATGTG 
GCCGTAGCTG TGCATCCCAA AGACCCCAGA TACCAGCACC TGAAGGGCAA GAGCGTGATC 
CACCCATTTG TGTCTCGGAG CCTCCCCATC GTCTTTGATG ACTTTGTGGA CATGGAGTTC 
GGCACAGGTG CGGTGAAGAT CACCCCCGCC CACGACCAGA ATGACTATGA GGTTGGGCAG 
CGGCACGGGC TGGAGGCTGT CAGCATTATG GACGCCCGCG GGGCCCTCGT CAACGTGCCC 
CCACCTTTCC TGGGCCTGCC CAGGTTTGAG GCCAGAAAGG CGGTGCTGGC AGCGCTCAAG 
GAGCAGGGGC TGTTCCGCGG CATTGAGGAC AACCCCATGG TGGTGCCACT TTGCAACCGC 
TCCAAGGACG TGGTGGAGCC TCTGCTGCGG CCGCAGTGGT ACGTGCGCTG TGGGGAGATG 
GCTCAGGCCG CCAGCGCTGC CGTGACACGG GGTGACCTCC GCATCCTCCC GGAGGCCCAT 
CAGCGGACGT GGCACGCCTG GATGGACAAC ATCCGGGACT GGTGTATCTC CCGGCAGCTG 
TGGTGGGGTC ATCGCATCCC AGCCTACTTC ATCACTGTCA ATGACCCCGC CGTGCCCCCA 
GGGGAGGACC CTGACGGGCG GTACTGGGTG AGCGGGCGCA CTGAGGCCGA GGCCCGGGAG 
AAGGCAGCCA AGGAGTTCTG CGTGTCCCCT GACAAGATCA GTCTCCAGCA AGATGAAGAT 
GTACTGGACA CCTGGTTCTC CTCTGGCCTC TTCCCCTTCT CCATCCTAGG CTGGCCCAAC 
CAGTCAGAAG ATCTGAGTGT GTTCTACCCG GGGACGCTGC TGGAGACAGG CCATGACATC 
CTCTTCTTCT GGGTGGCCAG GATGGTCATG CTTGGCCTCA AGCTCACTGG CAAGCTGCCC 
TTCAAAGAGG TCTACCTCCA TGCCATCGTG CGGGACGCCC ACGGCCGGAA GATGAGCAAG 
TCTCTAGGCA ATGTCATTGA CCCCCTGGAC GTTATCCATG GGGTCACCCT GCAGGGTCTC 
CACGACCAGT TACTGAATAG CAACCTGGAT CCCAGCGAGG TGGAGAAGGC AAAAGAGGGG 
CAGAAGGCAG ATTTCCCGAC GGGGATCCCC GAGTGTGGCA CCGATGCGCT CCGGTTTGGA 
CTATGCGCCT ACACGTCCCA GGGCCGTGAC ATCAACCTGG ATGTGAACCG GATATTGGGG 
TACCGCCATT TCTGCAACAA GCTCTGGAAC GCCACCAAGT TTGCCCTCCG TGGCCTTGGG 
AAGGGTTTCG TGCCCTCACC CACGTCTGAG CCTGGAGGCC GCGAGAGTCT GGTGGACCGC 
TGGATCCGCA GCCGGCTGAC CGAAGCTGTG AGGCTCAGTA ACCAAGGTTT CCAGGCCTAC 
GACTTCCCGG CTGTCACCAC CGCCCAGTAC AGCTTCTGGC TCTATGAGCT CTGTGATGTC 
TACCTGGAGT GCCTTAAGCC TGTGCTGAAT GGGGTGGACC AGGTAGCAGC CGAGTGCGCC 
CGCCAGACCC TCTACACCTG CCTGGACGTG GGCCTGCGGC TACTCTCACC TTTCATGCCC 
TTCGTGACTG AGGAGCTGTT CCAGCGGCTG CCCCGGCGGA CACCACAAGC TCCCCCTAGC 
CTCTGCGTCA CTGCCTACCC AGAGCCCTCA GAGTGCTCCT GGAAGGACCC TGAGGCAGAA 
GCTGCCTTCG AGCTGGCCCT GAGCATCACT CGAGCCGTGC GCTCCCTGCG TGCCGACTAC 
AACCTTACCC GGATCCGGCC TGACTGTTTC CTGGAAGTGG CTGACGAGGC CACAGGTGCC 
CTGGCATCAG CGGTGTCGGG CTACGTGCAG ACACTGGCCA GCGCGGGGAT TGTGGCCGTC 
CTGGCGCTGG GGGCTTCTGC ACCCCAGGGC TGCGCTGTGG CCCTGGCCTC TGACCGCTGC 
TCCGTCCACC TGCAGCTGCA GGGGCTAGTA GACCCAGCCC GGGAGCTGGG CAAACTGCAG 
ACCAAGCGTG ACGAGGCGCA GCGGCAGGCC CAGCGTCTGC GGGAGCGCCG TGCCGCCTCA 
GGCTACACTG TCAAGGTGCC CCTCAAAGTC CAGGAGGCAG ATGAAGCCAA GCTCCAGCAG 
ACAGAAGCAG AGCTCAGGAA GGTTGATGAG GCCATTGCCC TGTTTCAGAA GATGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_024984082.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839850.1 Bos taurus valyl-tRNA synthetase (VARS), transcript variant X3, mRNA.

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CloneID	OBa229800
Clone ID Related Accession (Same CDS sequence)	XM_024984082.1
Accession Version	XM_024984082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	valine--tRNA ligase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATTC TGTACGTCTC TCCTCACCCC GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCTGCTCGCT ACGGGGAAGC TGGGGAGGGT CCGGGATGGG GAGGAGCCCA CCCCCGCGTC 
TGTCTCCAGC CGCCCCCCGC CAGCAGGACT CCTTTTCCTC CACCCCGTCT GCCAGCCCTG 
GAACAGGGTC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGTCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGTCC CGGGGGTAGT CGGGCAGCCG TCCTGGTCCA ACAGTGGGTC 
AGTTACGCTG ACACCGAGCT AATACCAGCT GCCTGTGGGG CTACGCTGCC GGCCTTGGGA 
CTCCGAAGCT CGGCCCAAGA CCCTCAGGCT GCACTGGCGG CCCTGGGCAG AGCCCTGAGT 
CCCTTGGAAG AATGGCTTCG ACTGCATACC TACCTGGCCG GGGAGGCCCC AACTCTGGCT 
GACCTGGCAG CTGTCACAGC CTTGCTGCTG CCTTTCCGTT ATGTCCTGGA CCCCTCTGCC 
CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTATCACAT GTGTTCAGCA GCCAGAATTC 
CGGGCCGTAC TGGGAGAAGT GGTTCTGTAT TCAGGGGCCA GGTCTCTCTC CCAACAGCCA 
GGCTCTGAGG TCCCTGCACC CCCCAAAACG GCTGCTCAAC TCAAGAAAGA GGCAAAGAAA 
CGGGAAAAAC TAGAGAAATT CCAGCAGAAG CAGAAAGTCC AACAGCAGCA GCCACCCCCT 
GGGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGAGAAAC GGGATCCTGG GGTCATTACC 
TACGATCTCC CGACCCCACC TGGGGAAAAG AAAGATGTCA GTGGCACCAT GCCTGACTCC 
TACAGCCCTC AGTACGTGGA GGCTGCCTGG TACCCTTGGT GGGAGCAGCA GGGCTTCTTC 
AGGCCTGAGT ATGGGCGTTC CAGCGTGTCA GCACCAAACC CCCGGGGCAT CTTCATGATG 
TGCATCCCAC CCCCCAACGT GACAGGCTCC CTGCACCTGG GCCATGCGCT CACCAACGCC 
ATCCAGGACT CCCTGACCCG CTGGCACCGC ATGCGCGGAG AGACCACCCT GTGGAACCCT 
GGCTGTGACC ACGCGGGCAT TGCCACCCAG GTGGTGGTGG AGAAGAAACT CTGGCGCGAG 
CAGGGGCTGA GTCGGCACCA GCTGGGGCGA GAGGCTTTCC TGCAGGAGGT CTGGAAGTGG 
AAGGAGGAGA AAGGTGACCG GATTTACCAC CAGCTGAAGA AGCTTGGCAG CTCCTTGGAC 
TGGAATCGAG CCTGTTTCAC CATGGATCCT AAACTGTCAG CAGCCGTGAC GGAGGCCTTT 
GTACGGCTCC ACGAGGAGGG AATCATTTAC CGCAGCACCC GCCTTGTCAA CTGGTCCTGC 
ACCCTCAACT CTGCCATCTC CGACATCGAG GTGGATAAGA AGGAGCTGAC AGGTCGCACA 
CTGCTCTCCG TGCCTGGCTA CAAAGAGAAG GTGGAGTTTG GGGTCCTTGT CTCCTTTGCT 
TACAAGGTCC AAGGCTCAGA CAGCAACGAC GAGGTGGTAG TGGCAACCAC TCGGATCGAG 
ACGATGCTGG GAGATGTGGC CGTAGCTGTG CATCCCAAAG ACCCCAGATA CCAGCACCTG 
AAGGGCAAGA GCGTGATCCA CCCATTTGTG TCTCGGAGCC TCCCCATCGT CTTTGATGAC 
TTTGTGGACA TGGAGTTCGG CACAGGTGCG GTGAAGATCA CCCCCGCCCA CGACCAGAAT 
GACTATGAGG TTGGGCAGCG GCACGGGCTG GAGGCTGTCA GCATTATGGA CGCCCGCGGG 
GCCCTCGTCA ACGTGCCCCC ACCTTTCCTG GGCCTGCCCA GGTTTGAGGC CAGAAAGGCG 
GTGCTGGCAG CGCTCAAGGA GCAGGGGCTG TTCCGCGGCA TTGAGGACAA CCCCATGGTG 
GTGCCACTTT GCAACCGCTC CAAGGACGTG GTGGAGCCTC TGCTGCGGCC GCAGTGGTAC 
GTGCGCTGTG GGGAGATGGC TCAGGCCGCC AGCGCTGCCG TGACACGGGG TGACCTCCGC 
ATCCTCCCGG AGGCCCATCA GCGGACGTGG CACGCCTGGA TGGACAACAT CCGGGACTGG 
TGTATCTCCC GGCAGCTGTG GTGGGGTCAT CGCATCCCAG CCTACTTCAT CACTGTCAAT 
GACCCCGCCG TGCCCCCAGG GGAGGACCCT GACGGGCGGT ACTGGGTGAG CGGGCGCACT 
GAGGCCGAGG CCCGGGAGAA GGCAGCCAAG GAGTTCTGCG TGTCCCCTGA CAAGATCAGT 
CTCCAGCAAG ATGAAGATGT ACTGGACACC TGGTTCTCCT CTGGCCTCTT CCCCTTCTCC 
ATCCTAGGCT GGCCCAACCA GTCAGAAGAT CTGAGTGTGT TCTACCCGGG GACGCTGCTG 
GAGACAGGCC ATGACATCCT CTTCTTCTGG GTGGCCAGGA TGGTCATGCT TGGCCTCAAG 
CTCACTGGCA AGCTGCCCTT CAAAGAGGTC TACCTCCATG CCATCGTGCG GGACGCCCAC 
GGCCGGAAGA TGAGCAAGTC TCTAGGCAAT GTCATTGACC CCCTGGACGT TATCCATGGG 
GTCACCCTGC AGGGTCTCCA CGACCAGTTA CTGAATAGCA ACCTGGATCC CAGCGAGGTG 
GAGAAGGCAA AAGAGGGGCA GAAGGCAGAT TTCCCGACGG GGATCCCCGA GTGTGGCACC 
GATGCGCTCC GGTTTGGACT ATGCGCCTAC ACGTCCCAGG GCCGTGACAT CAACCTGGAT 
GTGAACCGGA TATTGGGGTA CCGCCATTTC TGCAACAAGC TCTGGAACGC CACCAAGTTT 
GCCCTCCGTG GCCTTGGGAA GGGTTTCGTG CCCTCACCCA CGTCTGAGCC TGGAGGCCGC 
GAGAGTCTGG TGGACCGCTG GATCCGCAGC CGGCTGACCG AAGCTGTGAG GCTCAGTAAC 
CAAGGTTTCC AGGCCTACGA CTTCCCGGCT GTCACCACCG CCCAGTACAG CTTCTGGCTC 
TATGAGCTCT GTGATGTCTA CCTGGAGTGC CTTAAGCCTG TGCTGAATGG GGTGGACCAG 
GTAGCAGCCG AGTGCGCCCG CCAGACCCTC TACACCTGCC TGGACGTGGG CCTGCGGCTA 
CTCTCACCTT TCATGCCCTT CGTGACTGAG GAGCTGTTCC AGCGGCTGCC CCGGCGGACA 
CCACAAGCTC CCCCTAGCCT CTGCGTCACT GCCTACCCAG AGCCCTCAGA GTGCTCCTGG 
AAGGACCCTG AGGCAGAAGC TGCCTTCGAG CTGGCCCTGA GCATCACTCG AGCCGTGCGC 
TCCCTGCGTG CCGACTACAA CCTTACCCGG ATCCGGCCTG ACTGTTTCCT GGAAGTGGCT 
GACGAGGCCA CAGGTGCCCT GGCATCAGCG GTGTCGGGCT ACGTGCAGAC ACTGGCCAGC 
GCGGGGATTG TGGCCGTCCT GGCGCTGGGG GCTTCTGCAC CCCAGGGCTG CGCTGTGGCC 
CTGGCCTCTG ACCGCTGCTC CGTCCACCTG CAGCTGCAGG GGCTAGTAGA CCCAGCCCGG 
GAGCTGGGCA AACTGCAGAC CAAGCGTGAC GAGGCGCAGC GGCAGGCCCA GCGTCTGCGG 
GAGCGCCGTG CCGCCTCAGG CTACACTGTC AAGGTGCCCC TCAAAGTCCA GGAGGCAGAT 
GAAGCCAAGC TCCAGCAGAC AGAAGCAGAG CTCAGGAAGG TTGATGAGGC CATTGCCCTG 
TTTCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839850.1
CDS	218..4015
Translation	MSILYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRVCLQPPPASRTPFPPPRLPALEQGPGGLWVWGATAVSQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAALAALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFITCVQQPEFRAVLGEVVLYSGARSLSQQPGSEVPAPPKTAAQLKKEAKKREKLEKFQQKQKVQQQQPPPGEQKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSNDEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAVSIMDARGALVNVPPPFLGLPRFEARKAVLAALKEQGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRTEAEAREKAAKEFCVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVTLQGLHDQLLNSNLDPSEVEKAKEGQKADFPTGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGRESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTAYPEPSECSWKDPEAEAAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRDEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_024984082.1
Organism	Bos taurus(cattle)
Definition	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984082.1

ORF Insert Sequence:

ATGTCCATTC TGTACGTCTC TCCTCACCCC GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCTGCTCGCT ACGGGGAAGC TGGGGAGGGT CCGGGATGGG GAGGAGCCCA CCCCCGCGTC 
TGTCTCCAGC CGCCCCCCGC CAGCAGGACT CCTTTTCCTC CACCCCGTCT GCCAGCCCTG 
GAACAGGGTC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGTCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGTCC CGGGGGTAGT CGGGCAGCCG TCCTGGTCCA ACAGTGGGTC 
AGTTACGCTG ACACCGAGCT AATACCAGCT GCCTGTGGGG CTACGCTGCC GGCCTTGGGA 
CTCCGAAGCT CGGCCCAAGA CCCTCAGGCT GCACTGGCGG CCCTGGGCAG AGCCCTGAGT 
CCCTTGGAAG AATGGCTTCG ACTGCATACC TACCTGGCCG GGGAGGCCCC AACTCTGGCT 
GACCTGGCAG CTGTCACAGC CTTGCTGCTG CCTTTCCGTT ATGTCCTGGA CCCCTCTGCC 
CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTATCACAT GTGTTCAGCA GCCAGAATTC 
CGGGCCGTAC TGGGAGAAGT GGTTCTGTAT TCAGGGGCCA GGTCTCTCTC CCAACAGCCA 
GGCTCTGAGG TCCCTGCACC CCCCAAAACG GCTGCTCAAC TCAAGAAAGA GGCAAAGAAA 
CGGGAAAAAC TAGAGAAATT CCAGCAGAAG CAGAAAGTCC AACAGCAGCA GCCACCCCCT 
GGGGAGCAGA AGAAACCAAA ACCAGAGAAG AGGGAGAAAC GGGATCCTGG GGTCATTACC 
TACGATCTCC CGACCCCACC TGGGGAAAAG AAAGATGTCA GTGGCACCAT GCCTGACTCC 
TACAGCCCTC AGTACGTGGA GGCTGCCTGG TACCCTTGGT GGGAGCAGCA GGGCTTCTTC 
AGGCCTGAGT ATGGGCGTTC CAGCGTGTCA GCACCAAACC CCCGGGGCAT CTTCATGATG 
TGCATCCCAC CCCCCAACGT GACAGGCTCC CTGCACCTGG GCCATGCGCT CACCAACGCC 
ATCCAGGACT CCCTGACCCG CTGGCACCGC ATGCGCGGAG AGACCACCCT GTGGAACCCT 
GGCTGTGACC ACGCGGGCAT TGCCACCCAG GTGGTGGTGG AGAAGAAACT CTGGCGCGAG 
CAGGGGCTGA GTCGGCACCA GCTGGGGCGA GAGGCTTTCC TGCAGGAGGT CTGGAAGTGG 
AAGGAGGAGA AAGGTGACCG GATTTACCAC CAGCTGAAGA AGCTTGGCAG CTCCTTGGAC 
TGGAATCGAG CCTGTTTCAC CATGGATCCT AAACTGTCAG CAGCCGTGAC GGAGGCCTTT 
GTACGGCTCC ACGAGGAGGG AATCATTTAC CGCAGCACCC GCCTTGTCAA CTGGTCCTGC 
ACCCTCAACT CTGCCATCTC CGACATCGAG GTGGATAAGA AGGAGCTGAC AGGTCGCACA 
CTGCTCTCCG TGCCTGGCTA CAAAGAGAAG GTGGAGTTTG GGGTCCTTGT CTCCTTTGCT 
TACAAGGTCC AAGGCTCAGA CAGCAACGAC GAGGTGGTAG TGGCAACCAC TCGGATCGAG 
ACGATGCTGG GAGATGTGGC CGTAGCTGTG CATCCCAAAG ACCCCAGATA CCAGCACCTG 
AAGGGCAAGA GCGTGATCCA CCCATTTGTG TCTCGGAGCC TCCCCATCGT CTTTGATGAC 
TTTGTGGACA TGGAGTTCGG CACAGGTGCG GTGAAGATCA CCCCCGCCCA CGACCAGAAT 
GACTATGAGG TTGGGCAGCG GCACGGGCTG GAGGCTGTCA GCATTATGGA CGCCCGCGGG 
GCCCTCGTCA ACGTGCCCCC ACCTTTCCTG GGCCTGCCCA GGTTTGAGGC CAGAAAGGCG 
GTGCTGGCAG CGCTCAAGGA GCAGGGGCTG TTCCGCGGCA TTGAGGACAA CCCCATGGTG 
GTGCCACTTT GCAACCGCTC CAAGGACGTG GTGGAGCCTC TGCTGCGGCC GCAGTGGTAC 
GTGCGCTGTG GGGAGATGGC TCAGGCCGCC AGCGCTGCCG TGACACGGGG TGACCTCCGC 
ATCCTCCCGG AGGCCCATCA GCGGACGTGG CACGCCTGGA TGGACAACAT CCGGGACTGG 
TGTATCTCCC GGCAGCTGTG GTGGGGTCAT CGCATCCCAG CCTACTTCAT CACTGTCAAT 
GACCCCGCCG TGCCCCCAGG GGAGGACCCT GACGGGCGGT ACTGGGTGAG CGGGCGCACT 
GAGGCCGAGG CCCGGGAGAA GGCAGCCAAG GAGTTCTGCG TGTCCCCTGA CAAGATCAGT 
CTCCAGCAAG ATGAAGATGT ACTGGACACC TGGTTCTCCT CTGGCCTCTT CCCCTTCTCC 
ATCCTAGGCT GGCCCAACCA GTCAGAAGAT CTGAGTGTGT TCTACCCGGG GACGCTGCTG 
GAGACAGGCC ATGACATCCT CTTCTTCTGG GTGGCCAGGA TGGTCATGCT TGGCCTCAAG 
CTCACTGGCA AGCTGCCCTT CAAAGAGGTC TACCTCCATG CCATCGTGCG GGACGCCCAC 
GGCCGGAAGA TGAGCAAGTC TCTAGGCAAT GTCATTGACC CCCTGGACGT TATCCATGGG 
GTCACCCTGC AGGGTCTCCA CGACCAGTTA CTGAATAGCA ACCTGGATCC CAGCGAGGTG 
GAGAAGGCAA AAGAGGGGCA GAAGGCAGAT TTCCCGACGG GGATCCCCGA GTGTGGCACC 
GATGCGCTCC GGTTTGGACT ATGCGCCTAC ACGTCCCAGG GCCGTGACAT CAACCTGGAT 
GTGAACCGGA TATTGGGGTA CCGCCATTTC TGCAACAAGC TCTGGAACGC CACCAAGTTT 
GCCCTCCGTG GCCTTGGGAA GGGTTTCGTG CCCTCACCCA CGTCTGAGCC TGGAGGCCGC 
GAGAGTCTGG TGGACCGCTG GATCCGCAGC CGGCTGACCG AAGCTGTGAG GCTCAGTAAC 
CAAGGTTTCC AGGCCTACGA CTTCCCGGCT GTCACCACCG CCCAGTACAG CTTCTGGCTC 
TATGAGCTCT GTGATGTCTA CCTGGAGTGC CTTAAGCCTG TGCTGAATGG GGTGGACCAG 
GTAGCAGCCG AGTGCGCCCG CCAGACCCTC TACACCTGCC TGGACGTGGG CCTGCGGCTA 
CTCTCACCTT TCATGCCCTT CGTGACTGAG GAGCTGTTCC AGCGGCTGCC CCGGCGGACA 
CCACAAGCTC CCCCTAGCCT CTGCGTCACT GCCTACCCAG AGCCCTCAGA GTGCTCCTGG 
AAGGACCCTG AGGCAGAAGC TGCCTTCGAG CTGGCCCTGA GCATCACTCG AGCCGTGCGC 
TCCCTGCGTG CCGACTACAA CCTTACCCGG ATCCGGCCTG ACTGTTTCCT GGAAGTGGCT 
GACGAGGCCA CAGGTGCCCT GGCATCAGCG GTGTCGGGCT ACGTGCAGAC ACTGGCCAGC 
GCGGGGATTG TGGCCGTCCT GGCGCTGGGG GCTTCTGCAC CCCAGGGCTG CGCTGTGGCC 
CTGGCCTCTG ACCGCTGCTC CGTCCACCTG CAGCTGCAGG GGCTAGTAGA CCCAGCCCGG 
GAGCTGGGCA AACTGCAGAC CAAGCGTGAC GAGGCGCAGC GGCAGGCCCA GCGTCTGCGG 
GAGCGCCGTG CCGCCTCAGG CTACACTGTC AAGGTGCCCC TCAAAGTCCA GGAGGCAGAT 
GAAGCCAAGC TCCAGCAGAC AGAAGCAGAG CTCAGGAAGG TTGATGAGGC CATTGCCCTG 
TTTCAGAAGA TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS ( XM_024984083.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839851.1 Bos taurus valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.

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CloneID	OBa229801
Clone ID Related Accession (Same CDS sequence)	XM_024984083.1
Accession Version	XM_024984083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	valine--tRNA ligase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATTC TGTACGTCTC TCCTCACCCC GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCTGCTCGCT ACGGGGAAGC TGGGGAGGGT CCGGGATGGG GAGGAGCCCA CCCCCGCGTC 
TGTCTCCAGC CGCCCCCCGC CAGCAGGACT CCTTTTCCTC CACCCCGTCT GCCAGCCCTG 
GAACAGGGTC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGTCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGTCC CGGGGGTAGT CGGGCAGCCG TCCTGGTCCA ACAGTGGGTC 
AGTTACGCTG ACACCGAGCT AATACCAGCT GCCTGTGGGG CTACGCTGCC GGCCTTGGGA 
CTCCGAAGCT CGGCCCAAGA CCCTCAGGCT GCACTGGCGG CCCTGGGCAG AGCCCTGAGT 
CCCTTGGAAG AATGGCTTCG ACTGCATACC TACCTGGCCG GGGAGGCCCC AACTCTGGCT 
GACCTGGCAG CTGTCACAGC CTTGCTGCTG CCTTTCCGTT ATGTCCTGGA CCCCTCTGCC 
CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTATCACAT GTGTTCAGCA GCCAGAATTC 
CGGGCCGTAC TGGGAGAAGT GGTTCTGTAT TCAGGGGCCA GGTCTCTCTC CCAACAGCCA 
GGCTCTGAGG TCCCTGCACC CCCCAAAACG GCTGCTCAAC TCAAGAAAGA GGCAAAGAAA 
CGGGAAAAAC TAGAGAAATT CCAGCAGAAG CAGAAAGTCC AACAGCAGCA GCCACCCCCT 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAC 
GATCTCCCGA CCCCACCTGG GGAAAAGAAA GATGTCAGTG GCACCATGCC TGACTCCTAC 
AGCCCTCAGT ACGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAGG 
CCTGAGTATG GGCGTTCCAG CGTGTCAGCA CCAAACCCCC GGGGCATCTT CATGATGTGC 
ATCCCACCCC CCAACGTGAC AGGCTCCCTG CACCTGGGCC ATGCGCTCAC CAACGCCATC 
CAGGACTCCC TGACCCGCTG GCACCGCATG CGCGGAGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCACG CGGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTCTG GCGCGAGCAG 
GGGCTGAGTC GGCACCAGCT GGGGCGAGAG GCTTTCCTGC AGGAGGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG CTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
AATCGAGCCT GTTTCACCAT GGATCCTAAA CTGTCAGCAG CCGTGACGGA GGCCTTTGTA 
CGGCTCCACG AGGAGGGAAT CATTTACCGC AGCACCCGCC TTGTCAACTG GTCCTGCACC 
CTCAACTCTG CCATCTCCGA CATCGAGGTG GATAAGAAGG AGCTGACAGG TCGCACACTG 
CTCTCCGTGC CTGGCTACAA AGAGAAGGTG GAGTTTGGGG TCCTTGTCTC CTTTGCTTAC 
AAGGTCCAAG GCTCAGACAG CAACGACGAG GTGGTAGTGG CAACCACTCG GATCGAGACG 
ATGCTGGGAG ATGTGGCCGT AGCTGTGCAT CCCAAAGACC CCAGATACCA GCACCTGAAG 
GGCAAGAGCG TGATCCACCC ATTTGTGTCT CGGAGCCTCC CCATCGTCTT TGATGACTTT 
GTGGACATGG AGTTCGGCAC AGGTGCGGTG AAGATCACCC CCGCCCACGA CCAGAATGAC 
TATGAGGTTG GGCAGCGGCA CGGGCTGGAG GCTGTCAGCA TTATGGACGC CCGCGGGGCC 
CTCGTCAACG TGCCCCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG AAAGGCGGTG 
CTGGCAGCGC TCAAGGAGCA GGGGCTGTTC CGCGGCATTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGCTCCAA GGACGTGGTG GAGCCTCTGC TGCGGCCGCA GTGGTACGTG 
CGCTGTGGGG AGATGGCTCA GGCCGCCAGC GCTGCCGTGA CACGGGGTGA CCTCCGCATC 
CTCCCGGAGG CCCATCAGCG GACGTGGCAC GCCTGGATGG ACAACATCCG GGACTGGTGT 
ATCTCCCGGC AGCTGTGGTG GGGTCATCGC ATCCCAGCCT ACTTCATCAC TGTCAATGAC 
CCCGCCGTGC CCCCAGGGGA GGACCCTGAC GGGCGGTACT GGGTGAGCGG GCGCACTGAG 
GCCGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCTGCGTGT CCCCTGACAA GATCAGTCTC 
CAGCAAGATG AAGATGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC 
CTAGGCTGGC CCAACCAGTC AGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG 
ACAGGCCATG ACATCCTCTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTCAAGCTC 
ACTGGCAAGC TGCCCTTCAA AGAGGTCTAC CTCCATGCCA TCGTGCGGGA CGCCCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATTGACCCCC TGGACGTTAT CCATGGGGTC 
ACCCTGCAGG GTCTCCACGA CCAGTTACTG AATAGCAACC TGGATCCCAG CGAGGTGGAG 
AAGGCAAAAG AGGGGCAGAA GGCAGATTTC CCGACGGGGA TCCCCGAGTG TGGCACCGAT 
GCGCTCCGGT TTGGACTATG CGCCTACACG TCCCAGGGCC GTGACATCAA CCTGGATGTG 
AACCGGATAT TGGGGTACCG CCATTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC 
CTCCGTGGCC TTGGGAAGGG TTTCGTGCCC TCACCCACGT CTGAGCCTGG AGGCCGCGAG 
AGTCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACCGAAG CTGTGAGGCT CAGTAACCAA 
GGTTTCCAGG CCTACGACTT CCCGGCTGTC ACCACCGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTACCT GGAGTGCCTT AAGCCTGTGC TGAATGGGGT GGACCAGGTA 
GCAGCCGAGT GCGCCCGCCA GACCCTCTAC ACCTGCCTGG ACGTGGGCCT GCGGCTACTC 
TCACCTTTCA TGCCCTTCGT GACTGAGGAG CTGTTCCAGC GGCTGCCCCG GCGGACACCA 
CAAGCTCCCC CTAGCCTCTG CGTCACTGCC TACCCAGAGC CCTCAGAGTG CTCCTGGAAG 
GACCCTGAGG CAGAAGCTGC CTTCGAGCTG GCCCTGAGCA TCACTCGAGC CGTGCGCTCC 
CTGCGTGCCG ACTACAACCT TACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCTGAC 
GAGGCCACAG GTGCCCTGGC ATCAGCGGTG TCGGGCTACG TGCAGACACT GGCCAGCGCG 
GGGATTGTGG CCGTCCTGGC GCTGGGGGCT TCTGCACCCC AGGGCTGCGC TGTGGCCCTG 
GCCTCTGACC GCTGCTCCGT CCACCTGCAG CTGCAGGGGC TAGTAGACCC AGCCCGGGAG 
CTGGGCAAAC TGCAGACCAA GCGTGACGAG GCGCAGCGGC AGGCCCAGCG TCTGCGGGAG 
CGCCGTGCCG CCTCAGGCTA CACTGTCAAG GTGCCCCTCA AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AGCAGACAGA AGCAGAGCTC AGGAAGGTTG ATGAGGCCAT TGCCCTGTTT 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839851.1
CDS	358..4152
Translation	MSILYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRVCLQPPPASRTPFPPPRLPALEQGPGGLWVWGATAVSQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAALAALGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFITCVQQPEFRAVLGEVVLYSGARSLSQQPGSEVPAPPKTAAQLKKEAKKREKLEKFQQKQKVQQQQPPPGEKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWEQQGFFRPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWNRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSNDEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAVSIMDARGALVNVPPPFLGLPRFEARKAVLAALKEQGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRTEAEAREKAAKEFCVSPDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGKLPFKEVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVTLQGLHDQLLNSNLDPSEVEKAKEGQKADFPTGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGRESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRTPQAPPSLCVTAYPEPSECSWKDPEAEAAFELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQTLASAGIVAVLALGASAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRDEAQRQAQRLRERRAASGYTVKVPLKVQEADEAKLQQTEAELRKVDEAIALFQKML

Target ORF information:

RefSeq Version	XM_024984083.1
Organism	Bos taurus(cattle)
Definition	Bos taurus valyl-tRNA synthetase (VARS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984083.1

ORF Insert Sequence:

ATGTCCATTC TGTACGTCTC TCCTCACCCC GATGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCTGCTCGCT ACGGGGAAGC TGGGGAGGGT CCGGGATGGG GAGGAGCCCA CCCCCGCGTC 
TGTCTCCAGC CGCCCCCCGC CAGCAGGACT CCTTTTCCTC CACCCCGTCT GCCAGCCCTG 
GAACAGGGTC CCGGTGGGCT CTGGGTGTGG GGGGCCACGG CTGTGTCCCA GCTGCTGTGG 
CCAGCAGGCC TGGGGGGTCC CGGGGGTAGT CGGGCAGCCG TCCTGGTCCA ACAGTGGGTC 
AGTTACGCTG ACACCGAGCT AATACCAGCT GCCTGTGGGG CTACGCTGCC GGCCTTGGGA 
CTCCGAAGCT CGGCCCAAGA CCCTCAGGCT GCACTGGCGG CCCTGGGCAG AGCCCTGAGT 
CCCTTGGAAG AATGGCTTCG ACTGCATACC TACCTGGCCG GGGAGGCCCC AACTCTGGCT 
GACCTGGCAG CTGTCACAGC CTTGCTGCTG CCTTTCCGTT ATGTCCTGGA CCCCTCTGCC 
CGCCGGATCT GGGGTAATGT GACTCGCTGG TTTATCACAT GTGTTCAGCA GCCAGAATTC 
CGGGCCGTAC TGGGAGAAGT GGTTCTGTAT TCAGGGGCCA GGTCTCTCTC CCAACAGCCA 
GGCTCTGAGG TCCCTGCACC CCCCAAAACG GCTGCTCAAC TCAAGAAAGA GGCAAAGAAA 
CGGGAAAAAC TAGAGAAATT CCAGCAGAAG CAGAAAGTCC AACAGCAGCA GCCACCCCCT 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAGAAACGGG ATCCTGGGGT CATTACCTAC 
GATCTCCCGA CCCCACCTGG GGAAAAGAAA GATGTCAGTG GCACCATGCC TGACTCCTAC 
AGCCCTCAGT ACGTGGAGGC TGCCTGGTAC CCTTGGTGGG AGCAGCAGGG CTTCTTCAGG 
CCTGAGTATG GGCGTTCCAG CGTGTCAGCA CCAAACCCCC GGGGCATCTT CATGATGTGC 
ATCCCACCCC CCAACGTGAC AGGCTCCCTG CACCTGGGCC ATGCGCTCAC CAACGCCATC 
CAGGACTCCC TGACCCGCTG GCACCGCATG CGCGGAGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCACG CGGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAACTCTG GCGCGAGCAG 
GGGCTGAGTC GGCACCAGCT GGGGCGAGAG GCTTTCCTGC AGGAGGTCTG GAAGTGGAAG 
GAGGAGAAAG GTGACCGGAT TTACCACCAG CTGAAGAAGC TTGGCAGCTC CTTGGACTGG 
AATCGAGCCT GTTTCACCAT GGATCCTAAA CTGTCAGCAG CCGTGACGGA GGCCTTTGTA 
CGGCTCCACG AGGAGGGAAT CATTTACCGC AGCACCCGCC TTGTCAACTG GTCCTGCACC 
CTCAACTCTG CCATCTCCGA CATCGAGGTG GATAAGAAGG AGCTGACAGG TCGCACACTG 
CTCTCCGTGC CTGGCTACAA AGAGAAGGTG GAGTTTGGGG TCCTTGTCTC CTTTGCTTAC 
AAGGTCCAAG GCTCAGACAG CAACGACGAG GTGGTAGTGG CAACCACTCG GATCGAGACG 
ATGCTGGGAG ATGTGGCCGT AGCTGTGCAT CCCAAAGACC CCAGATACCA GCACCTGAAG 
GGCAAGAGCG TGATCCACCC ATTTGTGTCT CGGAGCCTCC CCATCGTCTT TGATGACTTT 
GTGGACATGG AGTTCGGCAC AGGTGCGGTG AAGATCACCC CCGCCCACGA CCAGAATGAC 
TATGAGGTTG GGCAGCGGCA CGGGCTGGAG GCTGTCAGCA TTATGGACGC CCGCGGGGCC 
CTCGTCAACG TGCCCCCACC TTTCCTGGGC CTGCCCAGGT TTGAGGCCAG AAAGGCGGTG 
CTGGCAGCGC TCAAGGAGCA GGGGCTGTTC CGCGGCATTG AGGACAACCC CATGGTGGTG 
CCACTTTGCA ACCGCTCCAA GGACGTGGTG GAGCCTCTGC TGCGGCCGCA GTGGTACGTG 
CGCTGTGGGG AGATGGCTCA GGCCGCCAGC GCTGCCGTGA CACGGGGTGA CCTCCGCATC 
CTCCCGGAGG CCCATCAGCG GACGTGGCAC GCCTGGATGG ACAACATCCG GGACTGGTGT 
ATCTCCCGGC AGCTGTGGTG GGGTCATCGC ATCCCAGCCT ACTTCATCAC TGTCAATGAC 
CCCGCCGTGC CCCCAGGGGA GGACCCTGAC GGGCGGTACT GGGTGAGCGG GCGCACTGAG 
GCCGAGGCCC GGGAGAAGGC AGCCAAGGAG TTCTGCGTGT CCCCTGACAA GATCAGTCTC 
CAGCAAGATG AAGATGTACT GGACACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC 
CTAGGCTGGC CCAACCAGTC AGAAGATCTG AGTGTGTTCT ACCCGGGGAC GCTGCTGGAG 
ACAGGCCATG ACATCCTCTT CTTCTGGGTG GCCAGGATGG TCATGCTTGG CCTCAAGCTC 
ACTGGCAAGC TGCCCTTCAA AGAGGTCTAC CTCCATGCCA TCGTGCGGGA CGCCCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATTGACCCCC TGGACGTTAT CCATGGGGTC 
ACCCTGCAGG GTCTCCACGA CCAGTTACTG AATAGCAACC TGGATCCCAG CGAGGTGGAG 
AAGGCAAAAG AGGGGCAGAA GGCAGATTTC CCGACGGGGA TCCCCGAGTG TGGCACCGAT 
GCGCTCCGGT TTGGACTATG CGCCTACACG TCCCAGGGCC GTGACATCAA CCTGGATGTG 
AACCGGATAT TGGGGTACCG CCATTTCTGC AACAAGCTCT GGAACGCCAC CAAGTTTGCC 
CTCCGTGGCC TTGGGAAGGG TTTCGTGCCC TCACCCACGT CTGAGCCTGG AGGCCGCGAG 
AGTCTGGTGG ACCGCTGGAT CCGCAGCCGG CTGACCGAAG CTGTGAGGCT CAGTAACCAA 
GGTTTCCAGG CCTACGACTT CCCGGCTGTC ACCACCGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTACCT GGAGTGCCTT AAGCCTGTGC TGAATGGGGT GGACCAGGTA 
GCAGCCGAGT GCGCCCGCCA GACCCTCTAC ACCTGCCTGG ACGTGGGCCT GCGGCTACTC 
TCACCTTTCA TGCCCTTCGT GACTGAGGAG CTGTTCCAGC GGCTGCCCCG GCGGACACCA 
CAAGCTCCCC CTAGCCTCTG CGTCACTGCC TACCCAGAGC CCTCAGAGTG CTCCTGGAAG 
GACCCTGAGG CAGAAGCTGC CTTCGAGCTG GCCCTGAGCA TCACTCGAGC CGTGCGCTCC 
CTGCGTGCCG ACTACAACCT TACCCGGATC CGGCCTGACT GTTTCCTGGA AGTGGCTGAC 
GAGGCCACAG GTGCCCTGGC ATCAGCGGTG TCGGGCTACG TGCAGACACT GGCCAGCGCG 
GGGATTGTGG CCGTCCTGGC GCTGGGGGCT TCTGCACCCC AGGGCTGCGC TGTGGCCCTG 
GCCTCTGACC GCTGCTCCGT CCACCTGCAG CTGCAGGGGC TAGTAGACCC AGCCCGGGAG 
CTGGGCAAAC TGCAGACCAA GCGTGACGAG GCGCAGCGGC AGGCCCAGCG TCTGCGGGAG 
CGCCGTGCCG CCTCAGGCTA CACTGTCAAG GTGCCCCTCA AAGTCCAGGA GGCAGATGAA 
GCCAAGCTCC AGCAGACAGA AGCAGAGCTC AGGAAGGTTG ATGAGGCCAT TGCCCTGTTT 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

VARS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報