RFC1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFC1
Entrez Gene ID	832261
Full Name	replication factor C1
Synonyms	AtRFC1,replication factor C 1,replication factor C1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001343715.1	NP_001330213.1	replication factor C1
NM_147883.6	NP_680188.1	replication factor C1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	RFC1	NP_680188.1
Oryza sativa (rice)	Os11g0572100	NP_001068123.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RFC1 plays critical roles in abscisic acid-mediated homologous recombination in Arabidopsis.
Title: Abscisic acid suppresses the highly occurred somatic homologous recombination in Arabidopsis rfc1 mutant.

required for double-strand break repair during meiotic homologous recombination
Title: Replication factor C1 (RFC1) is required for double-strand break repair during meiotic homologous recombination in Arabidopsis.

That RFC1 is found in divergent eukaryotes suggests a previously unrecognized and highly conserved role for DNA synthesis in discriminating between recombination pathways.
Title: The DNA replication factor RFC1 is required for interference-sensitive meiotic crossovers in Arabidopsis thaliana.

results suggest that RFC1 helps mediate genomic stability and transcriptional gene silencing in Arabidopsis thaliana
Title: DNA replication factor C1 mediates genomic stability and transcriptional gene silencing in Arabidopsis.

Dada suggest that AtRFC1 plays an important role in embryo development in Arabidopsis thaliana.
Title: Arabidopsis replication factor C subunit 1 plays an important role in embryogenesis.

The following RFC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19206	NM_147883.6 Latest version!	Arabidopsis thaliana replication factor C1 (RFC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAb41093	NM_001343715.1 Latest version!	Arabidopsis thaliana replication factor C1 (RFC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFC1 ( NM_147883.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_680188.1 Arabidopsis thaliana replication factor C1 (RFC1), mRNA.

Price & Availability↑

CloneID	OAb19206
Clone ID Related Accession (Same CDS sequence)	NM_147883.6
Accession Version	NM_147883.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	replication factor C1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_147883.5.

ATGTCGGATA TTAGGAAGTG GTTTATGAAA GCTCATGAAA AAGGGAATGG AAGTGCTCCG 
AAATCGACGT CTTCCAAGGC AGGTCCTGTG AAGAATGCTG CAGAGACTGC ACCAATAAAA 
TCAGAGCAGG CTAGTGAAGA CTTGGAAACT GCTGATAGAA GAAAAACGAG CAAGTATTTT 
GGTAAAGATA AAACAAAAGT TAAAGATGAG AAAGAGGTGG AAGCAATTCC TGCCAAGAGG 
AAACTTAAGA CTGAGTCTGA TGATTTAGTC AAGCCTCGTC CAAGAAAGGT TACAAAGGTT 
GTTGATGATG ATGATGATGA TTTTGATGTA CCTATTTCAA GGAAGACTCG TGATACCACT 
CCCAGCAAAA AACTAAAGTC GGGGTCAGGT AGAGGAATTG CAAGTAAAAC TGTTGATAAC 
GACGATGATG ATGATGGTGA AGATAAGGAG ACTCCTCTAA AATCTGCCGG AAGAGGCCGT 
GGCGGTAGAG CTGCTCCAGG GGCCTCAACT GGTGGAAGAG GTAGAGGTGG AGGGCGAGGT 
GGATTTATGA ATTTTGGTGA AAGGAAAGAC CCACCGCATA AAGGAGAAAA GGAAGTTCCT 
GAAGGCACCC CTGATTGCTT AGCTGGATTG ACATTTGTAA TTAGCGGAAC ACTTGACAGT 
TTAGAAAGAG AAGAAGCAGA AGATCTGATT AAGCGGCATG GTGGTCGTAT TACAGGCTCT 
GTTAGCAAGA AAACGACCTA CCTTTTGTGT GATGAAGACA TTGGCGGAAG GAAATCTGAA 
AAAGCTAAAG AGCTGGGCAC TAAGTTCCTT ACAGAGGATG GCCTATTTGA CATAATCCGG 
TCCTCTAAAC CTGTAAAAAA GTCTCTACCT GAAAGGTCAA ACAAAGGCAC TGAAAAAATC 
TGTGCACCAC CGAAGACAAG TCCTCAGAAA GAAGAAACAA GAGGAAAACC TTTGGCAAAA 
AGTTCGCCCA AAAAGGTCCC CCCGGCGAAA GGAAAAAATA AAATTATCGA GACTTCCTTG 
CCCTGGACGG AAAAATACAG ACCGAAGGTT CCAAATGAGA TTGTTGGGAA TCAGTCGTTG 
GTTACTCAAC TTCACAACTG GCTGTCCCAC TGGCATGATC AATTTGGAGG TACTGGAAGT 
AAAGGAAAGG GGAAGAAATT GAATGATGCT GGTTCCAAGA AAGCTGTACT TTTGAGTGGA 
ACACCTGGTA TTGGAAAAAC AACATCAGCA AAGTTGGTTA GTCAGATGCT TGGTTTCCAG 
GCAGTTGAGG TTAATGCTAG TGACAGTCGT GGAAAGGCGA ACTCGAATAT TGCCAAAGGA 
ATTGGTGGCA GCAATGCAAA TTCTGTAAAA GAACTTGTCA ATAACGAAGC CATGGCTGCC 
AACTTCGATA GGTCAAAGCA CCCGAAGACT GTTCTAATCA TGGATGAAGT AGATGGAATG 
TCTGCAGGCG ACAGGGGAGG TGTTGCAGAT CTTATTGCTA GCATTAAAAT ATCAAAGATT 
CCTATCATCT GCATTTGTAA TGACCGATAT AGTCAAAAAC TGAAGAGTTT AGTAAACTAC 
TGTCTTCCCC TCAATTACCG GAAGCCCACG AAACAGCAGA TGGCAAAAAG GCTGATGCAT 
ATTGCTAAGG CAGAAGGCCT TGAAATTAAT GAAATTGCCC TGGAAGAACT CGCTGAGAGA 
GTGAATGGTG ACATACGATT GGCCGTAAAT CAGCTCCAAT ATATGAGCCT GTCCATGTCA 
GTCATCAAAT ACGATGATAT CAGGCAGCGC CTGCTAAGCA GTGCGAAGGA TGAAGATATT 
TCCCCTTTCA CAGCTGTTGA CAAGTTGTTT GGTTACAATG GTGGGAAGCT GAGAATGGAT 
GAACGGATTG ACCTAAGCAT GAGTGATCCG GATTTGGTTC CCCTACTTAT ACAGGAAAAC 
TATCTCAATT ATAGGCCAAG CGGTAAAGAT GAGGCAAAAC GAATGGATTT ACTCGCTCGT 
GCTGCTGAAT CTATTGCCGA TGGAGATATT ATCAACGTGC AGATTCGAAG ATACAGACAA 
TGGCAGCTCT CTCAATCTTG CTGTGTTGCA TCTTCTATTC TCCCAGCTTC ATTGTTGCAT 
GGATCAAGGG AGGTACTCGA GCAGGGAGAG CGTAATTTCA ATAGATTTGG TGGGTGGCTT 
GGCAAGAATT CTACTGCGGG AAAAAATAGA CGGTTAATGG AGGATCTGCA TGTTCATGTC 
CTTGCTTCCC GTGAATCCAG CGCTGGAAGG GAGACACTTC GTGTTGATTA TCTTCCCCTT 
CTCTTGAGCC GCTTGACTAG CCCACTTCAG ACACTGCCTA AGGATGAAGC AGTTTCAGAA 
GTTGTTGATT TCATGAACTC CTACTCTATA AGCCAGGAGG ACTTTGATAC TATTTTGGAG 
TTGGGAAAAT TTAAGGGACG TGAAAATCCG ATGGAGGGTG TTCCACCTCC AGTTAAAGCA 
GCTTTGACCA AAAAATATAA TGAGATGAAT AAAACCAGAA TGGTACGTGT GGCGGACATG 
GTGCAGCTTC CGGGAGTGAA AAAGGCGCCA AAGAAGCGAA TAGCTGCTAT GCTGGAACCA 
ACTGTTGATA GTTTGAGAGA TGAAGATGGT GAGCCGTTAG CAGATAATGA AGAAGGAAAT 
GGATCAGACG CTGAAGAAGA CTCAGAAGAA GCAACCGATG GAGAAAAGCT GGAGTCCAAT 
CTTAAAAATC TGAATGCTAG AGGGATCCAA GTGGAATTGG ATTTGAAAGG AGCTGGAAGC 
TCAGGTTCAA GAAAGGCGGC TGGTAAAGGC AGAGGCAGAG GGAAAGCAGC TGATACGTCT 
GCTGAGAAGA AAGCTACAGG AAGAGGCTCT GGTGCCAAGA GAAAGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_680188.1
CDS	203..3073
Translation	MSDIRKWFMKAHEKGNGSAPKSTSSKAGPVKNAAETAPIKSEQASEDLETADRRKTSKYFGKDKTKVKDEKEVEAIPAKRKLKTESDDLVKPRPRKVTKVVDDDDDDFDVPISRKTRDTTPSKKLKSGSGRGIASKTVDNDDDDDGEDKETPLKSAGRGRGGRAAPGASTGGRGRGGGRGGFMNFGERKDPPHKGEKEVPEGTPDCLAGLTFVISGTLDSLEREEAEDLIKRHGGRITGSVSKKTTYLLCDEDIGGRKSEKAKELGTKFLTEDGLFDIIRSSKPVKKSLPERSNKGTEKICAPPKTSPQKEETRGKPLAKSSPKKVPPAKGKNKIIETSLPWTEKYRPKVPNEIVGNQSLVTQLHNWLSHWHDQFGGTGSKGKGKKLNDAGSKKAVLLSGTPGIGKTTSAKLVSQMLGFQAVEVNASDSRGKANSNIAKGIGGSNANSVKELVNNEAMAANFDRSKHPKTVLIMDEVDGMSAGDRGGVADLIASIKISKIPIICICNDRYSQKLKSLVNYCLPLNYRKPTKQQMAKRLMHIAKAEGLEINEIALEELAERVNGDIRLAVNQLQYMSLSMSVIKYDDIRQRLLSSAKDEDISPFTAVDKLFGYNGGKLRMDERIDLSMSDPDLVPLLIQENYLNYRPSGKDEAKRMDLLARAAESIADGDIINVQIRRYRQWQLSQSCCVASSILPASLLHGSREVLEQGERNFNRFGGWLGKNSTAGKNRRLMEDLHVHVLASRESSAGRETLRVDYLPLLLSRLTSPLQTLPKDEAVSEVVDFMNSYSISQEDFDTILELGKFKGRENPMEGVPPPVKAALTKKYNEMNKTRMVRVADMVQLPGVKKAPKKRIAAMLEPTVDSLRDEDGEPLADNEEGNGSDAEEDSEEATDGEKLESNLKNLNARGIQVELDLKGAGSSGSRKAAGKGRGRGKAADTSAEKKATGRGSGAKRKR

Target ORF information:

RefSeq Version	NM_147883.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana replication factor C1 (RFC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147883.6

ORF Insert Sequence:

ATGTCGGATA TTAGGAAGTG GTTTATGAAA GCTCATGAAA AAGGGAATGG AAGTGCTCCG 
AAATCGACGT CTTCCAAGGC AGGTCCTGTG AAGAATGCTG CAGAGACTGC ACCAATAAAA 
TCAGAGCAGG CTAGTGAAGA CTTGGAAACT GCTGATAGAA GAAAAACGAG CAAGTATTTT 
GGTAAAGATA AAACAAAAGT TAAAGATGAG AAAGAGGTGG AAGCAATTCC TGCCAAGAGG 
AAACTTAAGA CTGAGTCTGA TGATTTAGTC AAGCCTCGTC CAAGAAAGGT TACAAAGGTT 
GTTGATGATG ATGATGATGA TTTTGATGTA CCTATTTCAA GGAAGACTCG TGATACCACT 
CCCAGCAAAA AACTAAAGTC GGGGTCAGGT AGAGGAATTG CAAGTAAAAC TGTTGATAAC 
GACGATGATG ATGATGGTGA AGATAAGGAG ACTCCTCTAA AATCTGCCGG AAGAGGCCGT 
GGCGGTAGAG CTGCTCCAGG GGCCTCAACT GGTGGAAGAG GTAGAGGTGG AGGGCGAGGT 
GGATTTATGA ATTTTGGTGA AAGGAAAGAC CCACCGCATA AAGGAGAAAA GGAAGTTCCT 
GAAGGCACCC CTGATTGCTT AGCTGGATTG ACATTTGTAA TTAGCGGAAC ACTTGACAGT 
TTAGAAAGAG AAGAAGCAGA AGATCTGATT AAGCGGCATG GTGGTCGTAT TACAGGCTCT 
GTTAGCAAGA AAACGACCTA CCTTTTGTGT GATGAAGACA TTGGCGGAAG GAAATCTGAA 
AAAGCTAAAG AGCTGGGCAC TAAGTTCCTT ACAGAGGATG GCCTATTTGA CATAATCCGG 
TCCTCTAAAC CTGTAAAAAA GTCTCTACCT GAAAGGTCAA ACAAAGGCAC TGAAAAAATC 
TGTGCACCAC CGAAGACAAG TCCTCAGAAA GAAGAAACAA GAGGAAAACC TTTGGCAAAA 
AGTTCGCCCA AAAAGGTCCC CCCGGCGAAA GGAAAAAATA AAATTATCGA GACTTCCTTG 
CCCTGGACGG AAAAATACAG ACCGAAGGTT CCAAATGAGA TTGTTGGGAA TCAGTCGTTG 
GTTACTCAAC TTCACAACTG GCTGTCCCAC TGGCATGATC AATTTGGAGG TACTGGAAGT 
AAAGGAAAGG GGAAGAAATT GAATGATGCT GGTTCCAAGA AAGCTGTACT TTTGAGTGGA 
ACACCTGGTA TTGGAAAAAC AACATCAGCA AAGTTGGTTA GTCAGATGCT TGGTTTCCAG 
GCAGTTGAGG TTAATGCTAG TGACAGTCGT GGAAAGGCGA ACTCGAATAT TGCCAAAGGA 
ATTGGTGGCA GCAATGCAAA TTCTGTAAAA GAACTTGTCA ATAACGAAGC CATGGCTGCC 
AACTTCGATA GGTCAAAGCA CCCGAAGACT GTTCTAATCA TGGATGAAGT AGATGGAATG 
TCTGCAGGCG ACAGGGGAGG TGTTGCAGAT CTTATTGCTA GCATTAAAAT ATCAAAGATT 
CCTATCATCT GCATTTGTAA TGACCGATAT AGTCAAAAAC TGAAGAGTTT AGTAAACTAC 
TGTCTTCCCC TCAATTACCG GAAGCCCACG AAACAGCAGA TGGCAAAAAG GCTGATGCAT 
ATTGCTAAGG CAGAAGGCCT TGAAATTAAT GAAATTGCCC TGGAAGAACT CGCTGAGAGA 
GTGAATGGTG ACATACGATT GGCCGTAAAT CAGCTCCAAT ATATGAGCCT GTCCATGTCA 
GTCATCAAAT ACGATGATAT CAGGCAGCGC CTGCTAAGCA GTGCGAAGGA TGAAGATATT 
TCCCCTTTCA CAGCTGTTGA CAAGTTGTTT GGTTACAATG GTGGGAAGCT GAGAATGGAT 
GAACGGATTG ACCTAAGCAT GAGTGATCCG GATTTGGTTC CCCTACTTAT ACAGGAAAAC 
TATCTCAATT ATAGGCCAAG CGGTAAAGAT GAGGCAAAAC GAATGGATTT ACTCGCTCGT 
GCTGCTGAAT CTATTGCCGA TGGAGATATT ATCAACGTGC AGATTCGAAG ATACAGACAA 
TGGCAGCTCT CTCAATCTTG CTGTGTTGCA TCTTCTATTC TCCCAGCTTC ATTGTTGCAT 
GGATCAAGGG AGGTACTCGA GCAGGGAGAG CGTAATTTCA ATAGATTTGG TGGGTGGCTT 
GGCAAGAATT CTACTGCGGG AAAAAATAGA CGGTTAATGG AGGATCTGCA TGTTCATGTC 
CTTGCTTCCC GTGAATCCAG CGCTGGAAGG GAGACACTTC GTGTTGATTA TCTTCCCCTT 
CTCTTGAGCC GCTTGACTAG CCCACTTCAG ACACTGCCTA AGGATGAAGC AGTTTCAGAA 
GTTGTTGATT TCATGAACTC CTACTCTATA AGCCAGGAGG ACTTTGATAC TATTTTGGAG 
TTGGGAAAAT TTAAGGGACG TGAAAATCCG ATGGAGGGTG TTCCACCTCC AGTTAAAGCA 
GCTTTGACCA AAAAATATAA TGAGATGAAT AAAACCAGAA TGGTACGTGT GGCGGACATG 
GTGCAGCTTC CGGGAGTGAA AAAGGCGCCA AAGAAGCGAA TAGCTGCTAT GCTGGAACCA 
ACTGTTGATA GTTTGAGAGA TGAAGATGGT GAGCCGTTAG CAGATAATGA AGAAGGAAAT 
GGATCAGACG CTGAAGAAGA CTCAGAAGAA GCAACCGATG GAGAAAAGCT GGAGTCCAAT 
CTTAAAAATC TGAATGCTAG AGGGATCCAA GTGGAATTGG ATTTGAAAGG AGCTGGAAGC 
TCAGGTTCAA GAAAGGCGGC TGGTAAAGGC AGAGGCAGAG GGAAAGCAGC TGATACGTCT 
GCTGAGAAGA AAGCTACAGG AAGAGGCTCT GGTGCCAAGA GAAAGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFC1 ( NM_001343715.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330213.1 Arabidopsis thaliana replication factor C1 (RFC1), mRNA.

Price & Availability↑

CloneID	OAb41093
Clone ID Related Accession (Same CDS sequence)	NM_001343715.1
Accession Version	NM_001343715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	replication factor C1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTCGGATA TTAGGAAGTG GTTTATGAAA GCTCATGAAA AAGGGAATGG AAGTGCTCCG 
AAATCGACGT CTTCCAAGGC AGGTCCTGTG AAGAATGCTG CAGAGACTGC ACCAATAAAA 
TCAGAGCAGG CTAGTGAAGA CTTGGAAACT GCTGATAGAA GAAAAACGAG CAAGTATTTT 
GGTAAAGATA AAACAAAAGT TAAAGATGAG AAAGAGGTGG AAGCAATTCC TGCCAAGAGG 
AAACTTAAGA CTGAGTCTGA TGATTTAGTC AAGCCTCGTC CAAGAAAGGT TACAAAGGTT 
GTTGATGATG ATGATGATGA TTTTGATGTA CCTATTTCAA GGAAGACTCG TGATACCACT 
CCCAGCAAAA AACTAAAGTC GGGGTCAGGT AGAGGAATTG CAAGTAAAAC TGTTGATAAC 
GACGATGATG ATGATGGTGA AGATAAGGAG ACTCCTCTAA AATCTGCCGG AAGAGGCCGT 
GGCGGTAGAG CTGCTCCAGG GGCCTCAACT GGTGGAAGAG GTAGAGGTGG AGGGCGAGGT 
GGATTTATGA ATTTTGGTGA AAGGAAAGAC CCACCGCATA AAGGAGAAAA GGAAGTTCCT 
GAAGGCACCC CTGATTGCTT AGCTGGATTG ACATTTGTAA TTAGCGGAAC ACTTGACAGT 
TTAGAAAGAG AAGAAGCAGA AGATCTGATT AAGCGGCATG GTGGTCGTAT TACAGGCTCT 
GTTAGCAAGA AAACGACCTA CCTTTTGTGT GATGAAGACA TTGGCGGAAG GAAATCTGAA 
AAAGCTAAAG AGCTGGGCAC TAAGTTCCTT ACAGAGGATG GCCTATTTGA CATAATCCGG 
TCCTCTAAAC CTGTAAAAAA GTCTCTACCT GAAAGGTCAA ACAAAGGCAC TGAAAAAATC 
TGTGCACCAC CGAAGACAAG TCCTCAGAAA GAAGAAACAA GAGGAAAACC TTTGGCAAAA 
AGTTCGCCCA AAAAGGTCCC CCCGGCGAAA GGAAAAAATA AAATTATCGA GACTTCCTTG 
CCCTGGACGG AAAAATACAG ACCGAAGGTT CCAAATGAGA TTGTTGGGAA TCAGTCGTTG 
GTTACTCAAC TTCACAACTG GCTGTCCCAC TGGCATGATC AATTTGGAGG TACTGGAAGT 
AAAGGAAAGG GGAAGAAATT GAATGATGCT GGTTCCAAGA AAGCTGTACT TTTGAGTGGA 
ACACCTGGTA TTGGAAAAAC AACATCAGCA AAGTTGGTTA GTCAGATGCT TGGTTTCCAG 
GCAGTTGAGG TTAATGCTAG TGACAGTCGT GGAAAGGCGA ACTCGAATAT TGCCAAAGGA 
ATTGGTGGCA GCAATGCAAA TTCTGTAAAA GAACTTGTCA ATAACGAAGC CATGGCTGCC 
AACTTCGATA GGTCAAAGCA CCCGAAGACT GTTCTAATCA TGGATGAAGT AGATGGAATG 
TCTGCAGGCG ACAGGGGAGG TGTTGCAGAT CTTATTGCTA GCATTAAAAT ATCAAAGATT 
CCTATCATCT GCATTTGTAA TGACCGATAT AGTCAAAAAC TGAAGAGTTT AGTAAACTAC 
TGTCTTCCCC TCAATTACCG GAAGCCCACG AAACAGCAGA TGGCAAAAAG GCTGATGCAT 
ATTGCTAAGG CAGAAGGCCT TGAAATTAAT GAAATTGCCC TGGAAGAACT CGCTGAGAGA 
GTGAATGGTG ACATACGATT GGCCGTAAAT CAGCTCCAAT ATATGAGCCT GTCCATGTCA 
GTCATCAAAT ACGATGATAT CAGGCAGCGC CTGCTAAGCA GTGCGAAGGA TGAAGATATT 
TCCCCTTTCA CAGCTGTTGA CAAGTTGTTT GGTTACAATG GTGGGAAGCT GAGAATGGAT 
GAACGGATTG ACCTAAGCAT GAGTGATCCG GATTTGGTTC CCCTACTTAT ACAGGAAAAC 
TATCTCAATT ATAGGCCAAG CGGTAAAGAT GAGGCAAAAC GAATGGATTT ACTCGCTCGT 
GCTGCTGAAT CTATTGCCGA TGGAGATATT ATCAACGTGC AGATTCGAAG ATACAGACAA 
TGGCAGCTCT CTCAATCTTG CTGTGTTGCA TCTTCTATTC TCCCAGCTTC ATTGTTGCAT 
GGATCAAGGG AGGTACTCGA GCAGGGAGAG CGTAATTTCA ATAGATTTGG TGGGTGGCTT 
GGCAAGAATT CTACTGCGGG AAAAAATAGA CGGTTAATGG AGGATCTGCA TGTTCATGTC 
CTTGCTTCCC GTGAATCCAG CGCTGGAAGG GAGACACTTC GTGTTGATTA TCTTCCCCTT 
CTCTTGAGCC GCTTGACTAG CCCACTTCAG ACACTGCCTA AGGATGAAGC AGTTTCAGAA 
GTTGTTGATT TCATGAACTC CTACTCTATA AGCCAGGAGG ACTTTGATAC TATTTTGGAG 
TTGGGAAAAT TTAAGGGACG TGAAAATCCG ATGGAGGGTG TTCCACCTCC AGTTAAAGCA 
GCTTTGACCA AAAAATATAA TGAGATGAAT AAAACCAGAA TGGTACGTGT GGCGGACATG 
GTGCAGCTTC CGGGAGTGAA AAAGGCGCCA AAGAAGCGAA TAGCTGCTAT GCTGGAACCA 
ACTGTTGATA GTTTGAGAGA TGAAGATGGT GAGCCGTTAG CAGATAATGA AGAAGGAAAT 
GGATCAGACG CTGAAGAAGA CTCAGAAGAA GCAACCGATG GAGAAAAGCT GGAGTCCAAT 
CTTAAAAATC TGAATGCTAG AGGTTCGTCT TGTTACTGCT TTAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330213.1
CDS	203..2950
Translation	MSDIRKWFMKAHEKGNGSAPKSTSSKAGPVKNAAETAPIKSEQASEDLETADRRKTSKYFGKDKTKVKDEKEVEAIPAKRKLKTESDDLVKPRPRKVTKVVDDDDDDFDVPISRKTRDTTPSKKLKSGSGRGIASKTVDNDDDDDGEDKETPLKSAGRGRGGRAAPGASTGGRGRGGGRGGFMNFGERKDPPHKGEKEVPEGTPDCLAGLTFVISGTLDSLEREEAEDLIKRHGGRITGSVSKKTTYLLCDEDIGGRKSEKAKELGTKFLTEDGLFDIIRSSKPVKKSLPERSNKGTEKICAPPKTSPQKEETRGKPLAKSSPKKVPPAKGKNKIIETSLPWTEKYRPKVPNEIVGNQSLVTQLHNWLSHWHDQFGGTGSKGKGKKLNDAGSKKAVLLSGTPGIGKTTSAKLVSQMLGFQAVEVNASDSRGKANSNIAKGIGGSNANSVKELVNNEAMAANFDRSKHPKTVLIMDEVDGMSAGDRGGVADLIASIKISKIPIICICNDRYSQKLKSLVNYCLPLNYRKPTKQQMAKRLMHIAKAEGLEINEIALEELAERVNGDIRLAVNQLQYMSLSMSVIKYDDIRQRLLSSAKDEDISPFTAVDKLFGYNGGKLRMDERIDLSMSDPDLVPLLIQENYLNYRPSGKDEAKRMDLLARAAESIADGDIINVQIRRYRQWQLSQSCCVASSILPASLLHGSREVLEQGERNFNRFGGWLGKNSTAGKNRRLMEDLHVHVLASRESSAGRETLRVDYLPLLLSRLTSPLQTLPKDEAVSEVVDFMNSYSISQEDFDTILELGKFKGRENPMEGVPPPVKAALTKKYNEMNKTRMVRVADMVQLPGVKKAPKKRIAAMLEPTVDSLRDEDGEPLADNEEGNGSDAEEDSEEATDGEKLESNLKNLNARGSSCYCFR

Target ORF information:

RefSeq Version	NM_001343715.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana replication factor C1 (RFC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343715.1

ORF Insert Sequence:

ATGTCGGATA TTAGGAAGTG GTTTATGAAA GCTCATGAAA AAGGGAATGG AAGTGCTCCG 
AAATCGACGT CTTCCAAGGC AGGTCCTGTG AAGAATGCTG CAGAGACTGC ACCAATAAAA 
TCAGAGCAGG CTAGTGAAGA CTTGGAAACT GCTGATAGAA GAAAAACGAG CAAGTATTTT 
GGTAAAGATA AAACAAAAGT TAAAGATGAG AAAGAGGTGG AAGCAATTCC TGCCAAGAGG 
AAACTTAAGA CTGAGTCTGA TGATTTAGTC AAGCCTCGTC CAAGAAAGGT TACAAAGGTT 
GTTGATGATG ATGATGATGA TTTTGATGTA CCTATTTCAA GGAAGACTCG TGATACCACT 
CCCAGCAAAA AACTAAAGTC GGGGTCAGGT AGAGGAATTG CAAGTAAAAC TGTTGATAAC 
GACGATGATG ATGATGGTGA AGATAAGGAG ACTCCTCTAA AATCTGCCGG AAGAGGCCGT 
GGCGGTAGAG CTGCTCCAGG GGCCTCAACT GGTGGAAGAG GTAGAGGTGG AGGGCGAGGT 
GGATTTATGA ATTTTGGTGA AAGGAAAGAC CCACCGCATA AAGGAGAAAA GGAAGTTCCT 
GAAGGCACCC CTGATTGCTT AGCTGGATTG ACATTTGTAA TTAGCGGAAC ACTTGACAGT 
TTAGAAAGAG AAGAAGCAGA AGATCTGATT AAGCGGCATG GTGGTCGTAT TACAGGCTCT 
GTTAGCAAGA AAACGACCTA CCTTTTGTGT GATGAAGACA TTGGCGGAAG GAAATCTGAA 
AAAGCTAAAG AGCTGGGCAC TAAGTTCCTT ACAGAGGATG GCCTATTTGA CATAATCCGG 
TCCTCTAAAC CTGTAAAAAA GTCTCTACCT GAAAGGTCAA ACAAAGGCAC TGAAAAAATC 
TGTGCACCAC CGAAGACAAG TCCTCAGAAA GAAGAAACAA GAGGAAAACC TTTGGCAAAA 
AGTTCGCCCA AAAAGGTCCC CCCGGCGAAA GGAAAAAATA AAATTATCGA GACTTCCTTG 
CCCTGGACGG AAAAATACAG ACCGAAGGTT CCAAATGAGA TTGTTGGGAA TCAGTCGTTG 
GTTACTCAAC TTCACAACTG GCTGTCCCAC TGGCATGATC AATTTGGAGG TACTGGAAGT 
AAAGGAAAGG GGAAGAAATT GAATGATGCT GGTTCCAAGA AAGCTGTACT TTTGAGTGGA 
ACACCTGGTA TTGGAAAAAC AACATCAGCA AAGTTGGTTA GTCAGATGCT TGGTTTCCAG 
GCAGTTGAGG TTAATGCTAG TGACAGTCGT GGAAAGGCGA ACTCGAATAT TGCCAAAGGA 
ATTGGTGGCA GCAATGCAAA TTCTGTAAAA GAACTTGTCA ATAACGAAGC CATGGCTGCC 
AACTTCGATA GGTCAAAGCA CCCGAAGACT GTTCTAATCA TGGATGAAGT AGATGGAATG 
TCTGCAGGCG ACAGGGGAGG TGTTGCAGAT CTTATTGCTA GCATTAAAAT ATCAAAGATT 
CCTATCATCT GCATTTGTAA TGACCGATAT AGTCAAAAAC TGAAGAGTTT AGTAAACTAC 
TGTCTTCCCC TCAATTACCG GAAGCCCACG AAACAGCAGA TGGCAAAAAG GCTGATGCAT 
ATTGCTAAGG CAGAAGGCCT TGAAATTAAT GAAATTGCCC TGGAAGAACT CGCTGAGAGA 
GTGAATGGTG ACATACGATT GGCCGTAAAT CAGCTCCAAT ATATGAGCCT GTCCATGTCA 
GTCATCAAAT ACGATGATAT CAGGCAGCGC CTGCTAAGCA GTGCGAAGGA TGAAGATATT 
TCCCCTTTCA CAGCTGTTGA CAAGTTGTTT GGTTACAATG GTGGGAAGCT GAGAATGGAT 
GAACGGATTG ACCTAAGCAT GAGTGATCCG GATTTGGTTC CCCTACTTAT ACAGGAAAAC 
TATCTCAATT ATAGGCCAAG CGGTAAAGAT GAGGCAAAAC GAATGGATTT ACTCGCTCGT 
GCTGCTGAAT CTATTGCCGA TGGAGATATT ATCAACGTGC AGATTCGAAG ATACAGACAA 
TGGCAGCTCT CTCAATCTTG CTGTGTTGCA TCTTCTATTC TCCCAGCTTC ATTGTTGCAT 
GGATCAAGGG AGGTACTCGA GCAGGGAGAG CGTAATTTCA ATAGATTTGG TGGGTGGCTT 
GGCAAGAATT CTACTGCGGG AAAAAATAGA CGGTTAATGG AGGATCTGCA TGTTCATGTC 
CTTGCTTCCC GTGAATCCAG CGCTGGAAGG GAGACACTTC GTGTTGATTA TCTTCCCCTT 
CTCTTGAGCC GCTTGACTAG CCCACTTCAG ACACTGCCTA AGGATGAAGC AGTTTCAGAA 
GTTGTTGATT TCATGAACTC CTACTCTATA AGCCAGGAGG ACTTTGATAC TATTTTGGAG 
TTGGGAAAAT TTAAGGGACG TGAAAATCCG ATGGAGGGTG TTCCACCTCC AGTTAAAGCA 
GCTTTGACCA AAAAATATAA TGAGATGAAT AAAACCAGAA TGGTACGTGT GGCGGACATG 
GTGCAGCTTC CGGGAGTGAA AAAGGCGCCA AAGAAGCGAA TAGCTGCTAT GCTGGAACCA 
ACTGTTGATA GTTTGAGAGA TGAAGATGGT GAGCCGTTAG CAGATAATGA AGAAGGAAAT 
GGATCAGACG CTGAAGAAGA CTCAGAAGAA GCAACCGATG GAGAAAAGCT GGAGTCCAAT 
CTTAAAAATC TGAATGCTAG AGGTTCGTCT TGTTACTGCT TTAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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RFC1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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