NCU07528 cDNA ORF clone, Neurospora crassa OR74A

The following NCU07528 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07528 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf00984 XM_011395633.1
Latest version!
Neurospora crassa OR74A tRNA pseudouridine synthase, variant (NCU07528), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ONf10917 XM_011395632.1
Latest version!
Neurospora crassa OR74A tRNA pseudouridine synthase (NCU07528), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf00984
    Clone ID Related Accession (Same CDS sequence) XM_011395633.1
    Accession Version XM_011395633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product tRNA pseudouridine synthase, variant
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCGCCG ACGACACACC ACCTGTCCCT GCGTCAGCAG ACGCTGCTGA CCCTGTTGTC 
    GAGACCACAA ATGCCGAATC TAATGACGCG GCGGACAATG GCGACCGAAA CGACAGAGGC
    CAGAAGAGAG GACGAGGCCG GGACAACAGA AACCCAAACC GGGACCGGAA CAACCCCAAC
    AACATCAAGA CGGGCGGCTT TGGGAGCAAC AAAAGAGACT TCCTGAGCAA GCGCGACCAG
    GTTCAAGACA ACCGTGCTGC CAAGCGCCGC AAGGTGATTG ACGGAGCCGC CGACGGCAAC
    AGTTACATGA GCATCCCCTT CTCGACCGAC GAAATCGCCG CCGAGGAACG CCGACCCAAG
    CGCAAGGTTG CCGTCCTGAT CGGCTACGCT GGAACCGGTT ACCACGGCAT CCAGATCAAT
    CACAAGGAAA AGACCATTGA GGGCGATCTG TTCGCTGCCT TCGTCAAGGC CGGCGCCATC
    TCCAAGGCCA ATGCCGACGA CCCCAAGAAG TCCAGTCTCG TTCGATGCGC CCGTACCGAC
    AAGGGTGTTC ACGCCGCCGC CAACGTTTTG TCGCTCAAAC TGATCGTCGA GGACGAGGAC
    ATTGTCGACA AGATCAACAG CCACCTGCCC GACCAGATCA AAGTATGGGG CATCCAGCGT
    GTAACCAACG GTTTCAGCTG CTACCAAGCA TGCGATTCCA GATGGTACGA GTACCTCATG
    CCCAGCTACG CTCTTCTGCC GCCACAGCCA CAAAGCTTCC TCGGCAAGAA GATCATTGAT
    GCGGCCAAGG AGAAGGGTAC ATACGACGAG TACATGTATA GACTGGACGA CGTCAAGAAC
    TTTTGGGAGG AAGTCGAGAA GAACGACATC CAGCCCATTC TCGAGAAAAT GGACCCCGAG
    ATCAGAGCCG AAGTCATTCG CATTCTGCAC GACACCGCCG ACCAAGAACT CACCGACGAC
    GGAGAGCCCT CGAAGCCCAA TACCGATGTA GCCAAGGATG TTGAGATGAA GGATACCCCG
    GCGGAAGAGA CCACGACCAC CGAGCCCTCA AAGGACGGCG AGGTTGTTAC CACTGAGGCC
    ACCACCGAGG CCACCACCAC CGAGACCCAA CCCCCCGCCG CCAAGGTCGA AAGAGAGTTC
    ACTCCCGTCG AGCTCGCCGT CCGCGAAGTC AAAGCCGCCT ACGTCGCCGC CAAGCGTCGC
    TACCGCGTGA CCGCCCCGCG CCTCGAGAAG CTGCAAGAAG CGCTCAACCA ATACCTCGGC
    ACACACAACT TCCACAACTA CACCATTCTC AAGTCGCACA CCGATCCCTC GGCTCGTCGT
    CATATTAAGT CTTTCCAAGT CAACCCGACG CCCATCCGCA TCAACGACAC CGAGTGGCTG
    TCCCTCAAGG TGCACGGCCA GAGCTTCATG ATGCACCAGA TTCGCAAGAT GGTAGCCATG
    GCCGTCTTGA TCGTACGGTG CGGCGCGCCC ATGAGTCTTG TCAATGAGAG CTACGGGCCC
    CGCAGGATTT CTATTCCCAA AGCGCCTGGT CTGGGTCTGA TGCTGGAGAG GCCGGTGTTT
    GATGTGAGCA ACAAGAAGTT CAAGGAGTTG GGTAAGGAGC CGATTGATTT TGGCAAGTGG
    GATAAGGAGA TCCAGGCGTT CAAGGATGAG CATATCTACA AGAGGATGTT TGAGTTGGAG
    GAGAAGGAGA ATTCGTTCCA CCTCTTTTTC AACCAGATCG ATAACTTCCG TACGGATTAT
    TTCCTCTGGG TCACCCCGGG CGGAATCGAG GCGTCGCACG AGCGGTCGGA TCGCACGGCC
    GAGAAGATAC CCAAGGCTTT GCAGGCTGAG CTTGGAGATG AGGCTGATGG TGTTGCGGAG
    GATGGGTCAT CGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393935.1
    CDS65..1942
    Translation

    Target ORF information:

    RefSeq Version XM_011395633.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A tRNA pseudouridine synthase, variant (NCU07528), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011395633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCCGCCG ACGACACACC ACCTGTCCCT GCGTCAGCAG ACGCTGCTGA CCCTGTTGTC 
    GAGACCACAA ATGCCGAATC TAATGACGCG GCGGACAATG GCGACCGAAA CGACAGAGGC
    CAGAAGAGAG GACGAGGCCG GGACAACAGA AACCCAAACC GGGACCGGAA CAACCCCAAC
    AACATCAAGA CGGGCGGCTT TGGGAGCAAC AAAAGAGACT TCCTGAGCAA GCGCGACCAG
    GTTCAAGACA ACCGTGCTGC CAAGCGCCGC AAGGTGATTG ACGGAGCCGC CGACGGCAAC
    AGTTACATGA GCATCCCCTT CTCGACCGAC GAAATCGCCG CCGAGGAACG CCGACCCAAG
    CGCAAGGTTG CCGTCCTGAT CGGCTACGCT GGAACCGGTT ACCACGGCAT CCAGATCAAT
    CACAAGGAAA AGACCATTGA GGGCGATCTG TTCGCTGCCT TCGTCAAGGC CGGCGCCATC
    TCCAAGGCCA ATGCCGACGA CCCCAAGAAG TCCAGTCTCG TTCGATGCGC CCGTACCGAC
    AAGGGTGTTC ACGCCGCCGC CAACGTTTTG TCGCTCAAAC TGATCGTCGA GGACGAGGAC
    ATTGTCGACA AGATCAACAG CCACCTGCCC GACCAGATCA AAGTATGGGG CATCCAGCGT
    GTAACCAACG GTTTCAGCTG CTACCAAGCA TGCGATTCCA GATGGTACGA GTACCTCATG
    CCCAGCTACG CTCTTCTGCC GCCACAGCCA CAAAGCTTCC TCGGCAAGAA GATCATTGAT
    GCGGCCAAGG AGAAGGGTAC ATACGACGAG TACATGTATA GACTGGACGA CGTCAAGAAC
    TTTTGGGAGG AAGTCGAGAA GAACGACATC CAGCCCATTC TCGAGAAAAT GGACCCCGAG
    ATCAGAGCCG AAGTCATTCG CATTCTGCAC GACACCGCCG ACCAAGAACT CACCGACGAC
    GGAGAGCCCT CGAAGCCCAA TACCGATGTA GCCAAGGATG TTGAGATGAA GGATACCCCG
    GCGGAAGAGA CCACGACCAC CGAGCCCTCA AAGGACGGCG AGGTTGTTAC CACTGAGGCC
    ACCACCGAGG CCACCACCAC CGAGACCCAA CCCCCCGCCG CCAAGGTCGA AAGAGAGTTC
    ACTCCCGTCG AGCTCGCCGT CCGCGAAGTC AAAGCCGCCT ACGTCGCCGC CAAGCGTCGC
    TACCGCGTGA CCGCCCCGCG CCTCGAGAAG CTGCAAGAAG CGCTCAACCA ATACCTCGGC
    ACACACAACT TCCACAACTA CACCATTCTC AAGTCGCACA CCGATCCCTC GGCTCGTCGT
    CATATTAAGT CTTTCCAAGT CAACCCGACG CCCATCCGCA TCAACGACAC CGAGTGGCTG
    TCCCTCAAGG TGCACGGCCA GAGCTTCATG ATGCACCAGA TTCGCAAGAT GGTAGCCATG
    GCCGTCTTGA TCGTACGGTG CGGCGCGCCC ATGAGTCTTG TCAATGAGAG CTACGGGCCC
    CGCAGGATTT CTATTCCCAA AGCGCCTGGT CTGGGTCTGA TGCTGGAGAG GCCGGTGTTT
    GATGTGAGCA ACAAGAAGTT CAAGGAGTTG GGTAAGGAGC CGATTGATTT TGGCAAGTGG
    GATAAGGAGA TCCAGGCGTT CAAGGATGAG CATATCTACA AGAGGATGTT TGAGTTGGAG
    GAGAAGGAGA ATTCGTTCCA CCTCTTTTTC AACCAGATCG ATAACTTCCG TACGGATTAT
    TTCCTCTGGG TCACCCCGGG CGGAATCGAG GCGTCGCACG AGCGGTCGGA TCGCACGGCC
    GAGAAGATAC CCAAGGCTTT GCAGGCTGAG CTTGGAGATG AGGCTGATGG TGTTGCGGAG
    GATGGGTCAT CGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ONf10917
    Clone ID Related Accession (Same CDS sequence) XM_011395632.1
    Accession Version XM_011395632.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product tRNA pseudouridine synthase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCTATTCA CAAGTCGATT GCTATCAAGA TGGCCGCCGA CGACACACCA CCTGTCCCTG 
    CGTCAGCAGA CGCTGCTGAC CCTGTTGTCG AGACCACAAA TGCCGAATCT AATGACGCGG
    CGGACAATGG CGACCGAAAC GACAGAGGCC AGAAGAGAGG ACGAGGCCGG GACAACAGAA
    ACCCAAACCG GGACCGGAAC AACCCCAACA ACATCAAGAC GGGCGGCTTT GGGAGCAACA
    AGCTGTAGAA GAGACTTCCT GAGCAAGCGC GACCAGGTTC AAGACAACCG TGCTGCCAAG
    CGCCGCAAGG TGATTGACGG AGCCGCCGAC GGCAACAGTT ACATGAGCAT CCCCTTCTCG
    ACCGACGAAA TCGCCGCCGA GGAACGCCGA CCCAAGCGCA AGGTTGCCGT CCTGATCGGC
    TACGCTGGAA CCGGTTACCA CGGCATCCAG ATCAATCACA AGGAAAAGAC CATTGAGGGC
    GATCTGTTCG CTGCCTTCGT CAAGGCCGGC GCCATCTCCA AGGCCAATGC CGACGACCCC
    AAGAAGTCCA GTCTCGTTCG ATGCGCCCGT ACCGACAAGG GTGTTCACGC CGCCGCCAAC
    GTTTTGTCGC TCAAACTGAT CGTCGAGGAC GAGGACATTG TCGACAAGAT CAACAGCCAC
    CTGCCCGACC AGATCAAAGT ATGGGGCATC CAGCGTGTAA CCAACGGTTT CAGCTGCTAC
    CAAGCATGCG ATTCCAGATG GTACGAGTAC CTCATGCCCA GCTACGCTCT TCTGCCGCCA
    CAGCCACAAA GCTTCCTCGG CAAGAAGATC ATTGATGCGG CCAAGGAGAA GGGTACATAC
    GACGAGTACA TGTATAGACT GGACGACGTC AAGAACTTTT GGGAGGAAGT CGAGAAGAAC
    GACATCCAGC CCATTCTCGA GAAAATGGAC CCCGAGATCA GAGCCGAAGT CATTCGCATT
    CTGCACGACA CCGCCGACCA AGAACTCACC GACGACGGAG AGCCCTCGAA GCCCAATACC
    GATGTAGCCA AGGATGTTGA GATGAAGGAT ACCCCGGCGG AAGAGACCAC GACCACCGAG
    CCCTCAAAGG ACGGCGAGGT TGTTACCACT GAGGCCACCA CCGAGGCCAC CACCACCGAG
    ACCCAACCCC CCGCCGCCAA GGTCGAAAGA GAGTTCACTC CCGTCGAGCT CGCCGTCCGC
    GAAGTCAAAG CCGCCTACGT CGCCGCCAAG CGTCGCTACC GCGTGACCGC CCCGCGCCTC
    GAGAAGCTGC AAGAAGCGCT CAACCAATAC CTCGGCACAC ACAACTTCCA CAACTACACC
    ATTCTCAAGT CGCACACCGA TCCCTCGGCT CGTCGTCATA TTAAGTCTTT CCAAGTCAAC
    CCGACGCCCA TCCGCATCAA CGACACCGAG TGGCTGTCCC TCAAGGTGCA CGGCCAGAGC
    TTCATGATGC ACCAGATTCG CAAGATGGTA GCCATGGCCG TCTTGATCGT ACGGTGCGGC
    GCGCCCATGA GTCTTGTCAA TGAGAGCTAC GGGCCCCGCA GGATTTCTAT TCCCAAAGCG
    CCTGGTCTGG GTCTGATGCT GGAGAGGCCG GTGTTTGATG TGAGCAACAA GAAGTTCAAG
    GAGTTGGGTA AGGAGCCGAT TGATTTTGGC AAGTGGGATA AGGAGATCCA GGCGTTCAAG
    GATGAGCATA TCTACAAGAG GATGTTTGAG TTGGAGGAGA AGGAGAATTC GTTCCACCTC
    TTTTTCAACC AGATCGATAA CTTCCGTACG GATTATTTCC TCTGGGTCAC CCCGGGCGGA
    ATCGAGGCGT CGCACGAGCG GTCGGATCGC ACGGCCGAGA AGATACCCAA GGCTTTGCAG
    GCTGAGCTTG GAGATGAGGC TGATGGTGTT GCGGAGGATG GGTCATCGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393934.1
    CDS36..1949
    Translation

    Target ORF information:

    RefSeq Version XM_011395632.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A tRNA pseudouridine synthase (NCU07528), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011395632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCTATTCA CAAGTCGATT GCTATCAAGA TGGCCGCCGA CGACACACCA CCTGTCCCTG 
    CGTCAGCAGA CGCTGCTGAC CCTGTTGTCG AGACCACAAA TGCCGAATCT AATGACGCGG
    CGGACAATGG CGACCGAAAC GACAGAGGCC AGAAGAGAGG ACGAGGCCGG GACAACAGAA
    ACCCAAACCG GGACCGGAAC AACCCCAACA ACATCAAGAC GGGCGGCTTT GGGAGCAACA
    AGCTGTAGAA GAGACTTCCT GAGCAAGCGC GACCAGGTTC AAGACAACCG TGCTGCCAAG
    CGCCGCAAGG TGATTGACGG AGCCGCCGAC GGCAACAGTT ACATGAGCAT CCCCTTCTCG
    ACCGACGAAA TCGCCGCCGA GGAACGCCGA CCCAAGCGCA AGGTTGCCGT CCTGATCGGC
    TACGCTGGAA CCGGTTACCA CGGCATCCAG ATCAATCACA AGGAAAAGAC CATTGAGGGC
    GATCTGTTCG CTGCCTTCGT CAAGGCCGGC GCCATCTCCA AGGCCAATGC CGACGACCCC
    AAGAAGTCCA GTCTCGTTCG ATGCGCCCGT ACCGACAAGG GTGTTCACGC CGCCGCCAAC
    GTTTTGTCGC TCAAACTGAT CGTCGAGGAC GAGGACATTG TCGACAAGAT CAACAGCCAC
    CTGCCCGACC AGATCAAAGT ATGGGGCATC CAGCGTGTAA CCAACGGTTT CAGCTGCTAC
    CAAGCATGCG ATTCCAGATG GTACGAGTAC CTCATGCCCA GCTACGCTCT TCTGCCGCCA
    CAGCCACAAA GCTTCCTCGG CAAGAAGATC ATTGATGCGG CCAAGGAGAA GGGTACATAC
    GACGAGTACA TGTATAGACT GGACGACGTC AAGAACTTTT GGGAGGAAGT CGAGAAGAAC
    GACATCCAGC CCATTCTCGA GAAAATGGAC CCCGAGATCA GAGCCGAAGT CATTCGCATT
    CTGCACGACA CCGCCGACCA AGAACTCACC GACGACGGAG AGCCCTCGAA GCCCAATACC
    GATGTAGCCA AGGATGTTGA GATGAAGGAT ACCCCGGCGG AAGAGACCAC GACCACCGAG
    CCCTCAAAGG ACGGCGAGGT TGTTACCACT GAGGCCACCA CCGAGGCCAC CACCACCGAG
    ACCCAACCCC CCGCCGCCAA GGTCGAAAGA GAGTTCACTC CCGTCGAGCT CGCCGTCCGC
    GAAGTCAAAG CCGCCTACGT CGCCGCCAAG CGTCGCTACC GCGTGACCGC CCCGCGCCTC
    GAGAAGCTGC AAGAAGCGCT CAACCAATAC CTCGGCACAC ACAACTTCCA CAACTACACC
    ATTCTCAAGT CGCACACCGA TCCCTCGGCT CGTCGTCATA TTAAGTCTTT CCAAGTCAAC
    CCGACGCCCA TCCGCATCAA CGACACCGAG TGGCTGTCCC TCAAGGTGCA CGGCCAGAGC
    TTCATGATGC ACCAGATTCG CAAGATGGTA GCCATGGCCG TCTTGATCGT ACGGTGCGGC
    GCGCCCATGA GTCTTGTCAA TGAGAGCTAC GGGCCCCGCA GGATTTCTAT TCCCAAAGCG
    CCTGGTCTGG GTCTGATGCT GGAGAGGCCG GTGTTTGATG TGAGCAACAA GAAGTTCAAG
    GAGTTGGGTA AGGAGCCGAT TGATTTTGGC AAGTGGGATA AGGAGATCCA GGCGTTCAAG
    GATGAGCATA TCTACAAGAG GATGTTTGAG TTGGAGGAGA AGGAGAATTC GTTCCACCTC
    TTTTTCAACC AGATCGATAA CTTCCGTACG GATTATTTCC TCTGGGTCAC CCCGGGCGGA
    ATCGAGGCGT CGCACGAGCG GTCGGATCGC ACGGCCGAGA AGATACCCAA GGCTTTGCAG
    GCTGAGCTTG GAGATGAGGC TGATGGTGTT GCGGAGGATG GGTCATCGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B