ORF » Species Summary » Neurospora crassa OR74A » leu-2 cDNA ORF clone

leu-2 cDNA ORF clone, Neurospora crassa OR74A

The following leu-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the leu-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf04501 XM_952369.2
Latest version!		 Neurospora crassa OR74A 3-isopropylmalate dehydratase (leu-2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    leu-2 ( XM_952369.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957462.1 Neurospora crassa OR74A 3-isopropylmalate dehydratase (leu-2), mRNA.
    Price & Availability↑
    CloneID ONf04501
    Clone ID Related Accession (Same CDS sequence) XM_952369.2
    Accession Version XM_952369.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product 3-isopropylmalate dehydratase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_952369.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCGTCAG CAGAGAGTAC CCCCCGTACC CTTTACGACA AGGTCTTCCA GGCCCATATC 
    GTCGACGAGA AGCTTGACGG TACCATTCTC CTCTACATTG ACCGCCACTT GGTCCACGAA 
    GTCACCTCTC CTCAAGCTTT TGAGGGCCTC GAAAACGCCG GACGCCAAGT TCGCAGGCCA 
    GACTGCACCC TTGCTACCAC CGATCACAAT GTCCCCACCA CCTCCAGGAA AGCGCTGAAG 
    GATATCGCCA GCTTCATCAA GGAGGATGAT TCGAGGACGC AGTGCGTCAC CCTCGAGGAG 
    AACGTCAAGA AGTTCGGCAT CACATACTTT GGCCTCAGCG ACAAGAGGCA AGGTATCGTC 
    CACGTCATCG GCCCCGAGCA GGGCTTCACC CTTCCCGGCA CCACGGTCGT CTGCGGCGAC 
    TCCCATACTT CTACCCATGG CGCTTTCGGC GCTCTCGCTT TCGGTATCGG CACCTCCGAG 
    GTCGAGCACG TCCTCGCCAC CCAGTGCCTC ATCACCAAGC GCAGCAAGAA CATGAGGGTT 
    CAGGTCGACG GTGAGCTTGC GCCCGGTGTG AGTTCCAAGG ATGTCATCCT CCACGTTATT 
    GGTCAGATCG GTACTGCCGG CGGTACTGGT GCCGTCATCG AGTTCTGCGG CTCCGTCATC 
    CGCAGCCTCA GCATGGAGGC CCGCATGTCC ATCTGCAACA TGTCGATCGA GGCCGGCGCC 
    AGAGCCGGTA TGGTGGCTCC CGATGAGATC ACCTTTGAGT ACCTCAAGGG CAGGCCCCTT 
    GCCCCCCGCT ACGGATCCGA GGAGTGGAAC AAGGCCGTCA CCTACTGGAA GTCTCTCGCC 
    TCCGACCCCG ATGCCAAGTA CGATATCGAT GTCTTCATCG ACGGCAAGGA TATCATCCCC 
    ACTGTTACCT GGGGTACCTC CCCCGAGGAC GTTGTCCCCA TCACCGGTGT TGTTCCCGAC 
    CCCGAGACCT TCGCTACCGA GGGCAAGAAG GCTGCTGGCC GTCGCATGAT CGAGTACATG 
    GGCCTCGTTC CCGGCACCCC CATGGAGGAC ATTGAGGTCG ACAAGGTCTT CATCGGCTCC 
    TGCACCAACT CCCGTATCGA GGATCTCCGC GCTGCCGCCC AGGTCGTCAA GGGCAAGAAG 
    ATCGCCGCCA ACATCAAGCG CGCCCTCATC GTCCCCGGCT CCGGTCTCGT AAAGGACCAA 
    GCTGAGGCCG AGGGCCTTGA CAAGATCTTC CAGGAGGCCG GTTTCGAGTG GCGCGAGGCT 
    GGTTGCTCCA TGTGCCTTGG TATGAACCCC GACATCCTCT CCCCCAAGGA GCGTTGCGCC 
    TCCACCAGCA ACCGCAATTT CGAGGGTCGC CAGGGTGCCC AGGGCCGCAC TCATTTGATG 
    TCCCCCGTCA TGGCCGCCGC CGCCGCCATC GTCGGCAAGC TCGCCGACGT GCGCAAGCTC 
    ACCGACTACA AGTCCAGCCC GCATGTTGAG GCCGCTGTCA TTCCTGAAAC TACCTCCACC 
    GCCAAGGCCC ACACTGACGA GCGTATCGAG GAGGACGATG TCGAGAAGGA CAAGCTCGCC 
    GACCAGCCCC AGGACTCCTC TCCCCAAGTC AACACCTCGG TCTCCAAGTC CTCTGCTGGC 
    CTCCCCAAGT TCACCAACCT CAAGGGCATC GCTGCGCCCA TGGAGAAGGC CAACATCGAC 
    ACGGACGCCA TCATCCCCAA GCAGTTCCTC AAGACCATCA AGCGCACCGG TCTCGGCTCC 
    GCCCTCTTCT ACGAGATGCG TTACAACCCT GACGGCAGCG AGAACCCCAA CTTTGTCCTC 
    AACCGCGAGC CGTACCGCTC CGCCAAGATC ATCGTCTGCA CAGGCGCCAA CTTTGGCTGC 
    GGCTCCTCGC GCGAGCACGC CCCTTGGGCA CTCAACGACT TCGGCGTCAA GTCCGTCATC 
    GCCCCTTCCT TCGCCGACAT CTTCTTTAAC AACTCCTTCA AGAACGGCAT GCTGCCCATC 
    GCGATCAAGG ACCAAGCCGC TCTCGAGCGC GTGCACGCCG AGGCCGCGGC CGGCCGCGAG 
    ATCGAGATCG ACTTGCCCAA CCAGCTAATC AAGGATGCTG ACGGTAACAC CCTCTGCGAG 
    TTCGAGGTCG AGGAGTTCAG GAAGCACTGT CTCGTTAACG GGCTGGACGA TATCGGTCTG 
    ACAATGCAGA TGGAGGATAA GATTGCCGAG TACGAGGCGA AGATGAGCCA GCAGACGCCC 
    TGGTTGGATG GACGCGCGTA TCTCAAGAGG AAGGGACAGG GTGGCAAGTT GGTGGCTAAG 
    GCGGTCCCAG TACCGAAGAC TAACCGCGGG GAGGAGAAGA AGGAGCCGCT CGAGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_957462.1
    CDS641..2980
    Translation

    Target ORF information:

    RefSeq Version XM_952369.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A 3-isopropylmalate dehydratase (leu-2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_952369.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCCGTCAG CAGAGAGTAC CCCCCGTACC CTTTACGACA AGGTCTTCCA GGCCCATATC 
    GTCGACGAGA AGCTTGACGG TACCATTCTC CTCTACATTG ACCGCCACTT GGTCCACGAA 
    GTCACCTCTC CTCAAGCTTT TGAGGGCCTC GAAAACGCCG GACGCCAAGT TCGCAGGCCA 
    GACTGCACCC TTGCTACCAC CGATCACAAT GTCCCCACCA CCTCCAGGAA AGCGCTGAAG 
    GATATCGCCA GCTTCATCAA GGAGGATGAT TCGAGGACGC AGTGCGTCAC CCTCGAGGAG 
    AACGTCAAGA AGTTCGGCAT CACATACTTT GGCCTCAGCG ACAAGAGGCA AGGTATCGTC 
    CACGTCATCG GCCCCGAGCA GGGCTTCACC CTTCCCGGCA CCACGGTCGT CTGCGGCGAC 
    TCCCATACTT CTACCCATGG CGCTTTCGGC GCTCTCGCTT TCGGTATCGG CACCTCCGAG 
    GTCGAGCACG TCCTCGCCAC CCAGTGCCTC ATCACCAAGC GCAGCAAGAA CATGAGGGTT 
    CAGGTCGACG GTGAGCTTGC GCCCGGTGTG AGTTCCAAGG ATGTCATCCT CCACGTTATT 
    GGTCAGATCG GTACTGCCGG CGGTACTGGT GCCGTCATCG AGTTCTGCGG CTCCGTCATC 
    CGCAGCCTCA GCATGGAGGC CCGCATGTCC ATCTGCAACA TGTCGATCGA GGCCGGCGCC 
    AGAGCCGGTA TGGTGGCTCC CGATGAGATC ACCTTTGAGT ACCTCAAGGG CAGGCCCCTT 
    GCCCCCCGCT ACGGATCCGA GGAGTGGAAC AAGGCCGTCA CCTACTGGAA GTCTCTCGCC 
    TCCGACCCCG ATGCCAAGTA CGATATCGAT GTCTTCATCG ACGGCAAGGA TATCATCCCC 
    ACTGTTACCT GGGGTACCTC CCCCGAGGAC GTTGTCCCCA TCACCGGTGT TGTTCCCGAC 
    CCCGAGACCT TCGCTACCGA GGGCAAGAAG GCTGCTGGCC GTCGCATGAT CGAGTACATG 
    GGCCTCGTTC CCGGCACCCC CATGGAGGAC ATTGAGGTCG ACAAGGTCTT CATCGGCTCC 
    TGCACCAACT CCCGTATCGA GGATCTCCGC GCTGCCGCCC AGGTCGTCAA GGGCAAGAAG 
    ATCGCCGCCA ACATCAAGCG CGCCCTCATC GTCCCCGGCT CCGGTCTCGT AAAGGACCAA 
    GCTGAGGCCG AGGGCCTTGA CAAGATCTTC CAGGAGGCCG GTTTCGAGTG GCGCGAGGCT 
    GGTTGCTCCA TGTGCCTTGG TATGAACCCC GACATCCTCT CCCCCAAGGA GCGTTGCGCC 
    TCCACCAGCA ACCGCAATTT CGAGGGTCGC CAGGGTGCCC AGGGCCGCAC TCATTTGATG 
    TCCCCCGTCA TGGCCGCCGC CGCCGCCATC GTCGGCAAGC TCGCCGACGT GCGCAAGCTC 
    ACCGACTACA AGTCCAGCCC GCATGTTGAG GCCGCTGTCA TTCCTGAAAC TACCTCCACC 
    GCCAAGGCCC ACACTGACGA GCGTATCGAG GAGGACGATG TCGAGAAGGA CAAGCTCGCC 
    GACCAGCCCC AGGACTCCTC TCCCCAAGTC AACACCTCGG TCTCCAAGTC CTCTGCTGGC 
    CTCCCCAAGT TCACCAACCT CAAGGGCATC GCTGCGCCCA TGGAGAAGGC CAACATCGAC 
    ACGGACGCCA TCATCCCCAA GCAGTTCCTC AAGACCATCA AGCGCACCGG TCTCGGCTCC 
    GCCCTCTTCT ACGAGATGCG TTACAACCCT GACGGCAGCG AGAACCCCAA CTTTGTCCTC 
    AACCGCGAGC CGTACCGCTC CGCCAAGATC ATCGTCTGCA CAGGCGCCAA CTTTGGCTGC 
    GGCTCCTCGC GCGAGCACGC CCCTTGGGCA CTCAACGACT TCGGCGTCAA GTCCGTCATC 
    GCCCCTTCCT TCGCCGACAT CTTCTTTAAC AACTCCTTCA AGAACGGCAT GCTGCCCATC 
    GCGATCAAGG ACCAAGCCGC TCTCGAGCGC GTGCACGCCG AGGCCGCGGC CGGCCGCGAG 
    ATCGAGATCG ACTTGCCCAA CCAGCTAATC AAGGATGCTG ACGGTAACAC CCTCTGCGAG 
    TTCGAGGTCG AGGAGTTCAG GAAGCACTGT CTCGTTAACG GGCTGGACGA TATCGGTCTG 
    ACAATGCAGA TGGAGGATAA GATTGCCGAG TACGAGGCGA AGATGAGCCA GCAGACGCCC 
    TGGTTGGATG GACGCGCGTA TCTCAAGAGG AAGGGACAGG GTGGCAAGTT GGTGGCTAAG 
    GCGGTCCCAG TACCGAAGAC TAACCGCGGG GAGGAGAAGA AGGAGCCGCT CGAGTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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