ORF » Species Summary » Schizosaccharomyces pombe » leu2 cDNA ORF clone

leu2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following leu2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the leu2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102689 NM_001020005.2
Latest version!		 Schizosaccharomyces pombe putative 3-isopropylmalate dehydratase Leu2 (leu2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    leu2 ( NM_001020005.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594576.1 Schizosaccharomyces pombe putative 3-isopropylmalate dehydratase Leu2 (leu2), mRNA.
    Price & Availability↑
    CloneID OSc102689
    Clone ID Related Accession (Same CDS sequence) NM_001020005.2
    Accession Version NM_001020005.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative 3-isopropylmalate dehydratase Leu2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020005.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTCCCT CAGTTGCTTC TCCTAAAACT TTGTACGACA AGGTTTGGGA CAGCCACGTT 
    GTCGACCTTC AAGAGGATGG TACATGTTTG TTGTATATTG ATCGACACTT GATTCACGAA 
    GTCACTTCTC CTCAAGCCTT TGAGGGCCTT CGTACTGCTG GTAGAAAAGT TCGTCATCCC 
    GAACTTGCTC TTGCAACTGT CGATCACAAT ATTCCTACCG ATCCTCGTAA GGACATGAAG 
    GATATCGCTT CCTTCATTCA TCAACCTGAC TCACGCACTC AAGTCTTGGC TTTGGAAAAT 
    AACATTAAGG AGTTTGGTCT TACCTACTAT GGTATGAATG ACAGACGTCA AGGTATCGTT 
    CACGTTATTG GTCCTGAACA GGGCTTCACT CTCCCTGGTA CGACCCTCGT TTGTGGTGAT 
    TCTCACACCT CTACCCACGG CGCTTTTGGT GCTTTGGCTT TTGGTATCGG TACTAGTGAG 
    GTCGAACATG TCTTGGCCAC TCAAACCATT TTGCAACGCA AGAGTAAAAA CATGCGCATT 
    CGTGTAAACG GTAAATTGCC TGAGGGTATT GCTTCTAAGG ACTTGATCCT TCATATTATT 
    GGAGTTATTG GTACTGCTGG TGGTACTGGT TCCGTTATTG AGTTTTGCGG TGAAGCTATT 
    GAAGGTTTAA GTATGGAAGC TCGTATGTCC ATGTGTAACA TGTCCATTGA GGCTGGTGCT 
    CGCGCCGGTA TGATTGCTCC TGATGCAACG ACTTTTGAAT ATGTTAAAAA CCGCCCTCTT 
    GCTCCCAAGG GCGATGATTG GGAACAGGCC GTGGCTTATT GGAAAACCCT CCGCTCCGAT 
    GAGAATGCTA AGTACGATAT TGAAGTTGAA ATCAATGCTG CTGATGTTTT ACCCACTGTA 
    ACATGGGGTA CTTCTCCTCA GGACGTTATC CCCATTAATG GTAATATCCC TGATCCCGCA 
    CACGTTAAAG ACAACGTACG TGCTGCGTCT ATTCAACGCT CTCTAGAATA TATGGGACTC 
    AAACCTAATA CCTCTATTGT GTCCTATCCC ATCGACAAAG TCTTTATTGG CTCTTGTACC 
    AATTCTCGTA TTGAGGATTT GCGTCTTGCT GCTGCCGTTG TCAAGGGCCG TAAGGTCGCT 
    GCTAACGTAA AAGATGCTAT GATTGTTCCA GGCTCTGGTC TCGTTAAAAA AATGGCCGAA 
    GCTGAAGGAT TGGACCAAAT ATTCATTGAA GCTGGATTTG ATTGGCGTGA AGCTGGCTGT 
    TCAATGTGTT TGGGTATGAA TCCCGATCAA CTCAAGCCAT ATGAACGTTG TGCCTCAACC 
    TCAAATCGTA ACTTTGAAGG TCGCCAAGGT GCTAAGGGTA GAACGCATCT TGTTAGTCCC 
    GCTATGGCTG CCGCTGCCGC TATTAAAGGA CATCTCTGTA ATGTTCGTGA ATTTTTTGGA 
    GATGTTTCCA ACGGATCTCC TTCTATTATT ACTAACAAGA ACTATGATCC CAGCCATGAT 
    GTAGAGGGTG ATATAGGCCT TTCTGTTGAC GATGCTACTG ATGCAGTTAC TGATGCTGAT 
    GGCATTGCCA CTAATGTTGC TGGTTCTGTA TCTTCTGGAT CTGCCGGTAT TCCCAAATTC 
    ACCGTCGTTG AAGGCATTGC TGCTCCTTTG CCTATGGCTA ATGTCGACAC TGACAAAATT 
    ATTCCCAAGC AATTTTTGAA GACCATTAAA CGTACCGGTC TTGGTCAATT TGCTTTCTAC 
    GAAATCCGTT ACGATGCGGA CGGAAAGGAG ATACCCGACT TTGTCCTCAA TCGCGAACCT 
    TACCGACATG CAACTGTTTT GGTTGCTCAC GACAACTTCG GTTGTGGTAG CTCTCGTGAG 
    CATGCTCCAT GGGCATTAAA CGATTTTGGC ATTCGTGTAA TTATTGCACC GTCTTTTGCT 
    GACATTTTCT TCAATAACTG CTTCAAGAAT GGTATGCTTC CTATTCCTAC TCCAATTGAG 
    CAAGTTAATG ATATGATGAA AGCCGCTGAA AATCAAGTCA AATTCTCTGT CGATCTTGTT 
    AATCAAACTA TAACTTATGG TGATAAACAA GTCAAATTTG ATGTTGAGCC TTTCCGTAAA 
    CATTGTTTGG TTAATGGTTT GGATGACATC GGACTGACTT TGCAAAAAGA AACTATGATT 
    GACGCTTTTG AGGCTGCTAG AGAAGAAAAC TTCCCTTGGA TGAATATCAA GCGCAGCCGG 
    GCTCGCCTTT CACCTGTTAA AAGCAATAAG CAATCTTCTA GCAGGAACGA TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594576.1
    CDS260..2536
    Translation

    Target ORF information:

    RefSeq Version NM_001020005.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative 3-isopropylmalate dehydratase Leu2 (leu2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001020005.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTCCCT CAGTTGCTTC TCCTAAAACT TTGTACGACA AGGTTTGGGA CAGCCACGTT 
    GTCGACCTTC AAGAGGATGG TACATGTTTG TTGTATATTG ATCGACACTT GATTCACGAA 
    GTCACTTCTC CTCAAGCCTT TGAGGGCCTT CGTACTGCTG GTAGAAAAGT TCGTCATCCC 
    GAACTTGCTC TTGCAACTGT CGATCACAAT ATTCCTACCG ATCCTCGTAA GGACATGAAG 
    GATATCGCTT CCTTCATTCA TCAACCTGAC TCACGCACTC AAGTCTTGGC TTTGGAAAAT 
    AACATTAAGG AGTTTGGTCT TACCTACTAT GGTATGAATG ACAGACGTCA AGGTATCGTT 
    CACGTTATTG GTCCTGAACA GGGCTTCACT CTCCCTGGTA CGACCCTCGT TTGTGGTGAT 
    TCTCACACCT CTACCCACGG CGCTTTTGGT GCTTTGGCTT TTGGTATCGG TACTAGTGAG 
    GTCGAACATG TCTTGGCCAC TCAAACCATT TTGCAACGCA AGAGTAAAAA CATGCGCATT 
    CGTGTAAACG GTAAATTGCC TGAGGGTATT GCTTCTAAGG ACTTGATCCT TCATATTATT 
    GGAGTTATTG GTACTGCTGG TGGTACTGGT TCCGTTATTG AGTTTTGCGG TGAAGCTATT 
    GAAGGTTTAA GTATGGAAGC TCGTATGTCC ATGTGTAACA TGTCCATTGA GGCTGGTGCT 
    CGCGCCGGTA TGATTGCTCC TGATGCAACG ACTTTTGAAT ATGTTAAAAA CCGCCCTCTT 
    GCTCCCAAGG GCGATGATTG GGAACAGGCC GTGGCTTATT GGAAAACCCT CCGCTCCGAT 
    GAGAATGCTA AGTACGATAT TGAAGTTGAA ATCAATGCTG CTGATGTTTT ACCCACTGTA 
    ACATGGGGTA CTTCTCCTCA GGACGTTATC CCCATTAATG GTAATATCCC TGATCCCGCA 
    CACGTTAAAG ACAACGTACG TGCTGCGTCT ATTCAACGCT CTCTAGAATA TATGGGACTC 
    AAACCTAATA CCTCTATTGT GTCCTATCCC ATCGACAAAG TCTTTATTGG CTCTTGTACC 
    AATTCTCGTA TTGAGGATTT GCGTCTTGCT GCTGCCGTTG TCAAGGGCCG TAAGGTCGCT 
    GCTAACGTAA AAGATGCTAT GATTGTTCCA GGCTCTGGTC TCGTTAAAAA AATGGCCGAA 
    GCTGAAGGAT TGGACCAAAT ATTCATTGAA GCTGGATTTG ATTGGCGTGA AGCTGGCTGT 
    TCAATGTGTT TGGGTATGAA TCCCGATCAA CTCAAGCCAT ATGAACGTTG TGCCTCAACC 
    TCAAATCGTA ACTTTGAAGG TCGCCAAGGT GCTAAGGGTA GAACGCATCT TGTTAGTCCC 
    GCTATGGCTG CCGCTGCCGC TATTAAAGGA CATCTCTGTA ATGTTCGTGA ATTTTTTGGA 
    GATGTTTCCA ACGGATCTCC TTCTATTATT ACTAACAAGA ACTATGATCC CAGCCATGAT 
    GTAGAGGGTG ATATAGGCCT TTCTGTTGAC GATGCTACTG ATGCAGTTAC TGATGCTGAT 
    GGCATTGCCA CTAATGTTGC TGGTTCTGTA TCTTCTGGAT CTGCCGGTAT TCCCAAATTC 
    ACCGTCGTTG AAGGCATTGC TGCTCCTTTG CCTATGGCTA ATGTCGACAC TGACAAAATT 
    ATTCCCAAGC AATTTTTGAA GACCATTAAA CGTACCGGTC TTGGTCAATT TGCTTTCTAC 
    GAAATCCGTT ACGATGCGGA CGGAAAGGAG ATACCCGACT TTGTCCTCAA TCGCGAACCT 
    TACCGACATG CAACTGTTTT GGTTGCTCAC GACAACTTCG GTTGTGGTAG CTCTCGTGAG 
    CATGCTCCAT GGGCATTAAA CGATTTTGGC ATTCGTGTAA TTATTGCACC GTCTTTTGCT 
    GACATTTTCT TCAATAACTG CTTCAAGAAT GGTATGCTTC CTATTCCTAC TCCAATTGAG 
    CAAGTTAATG ATATGATGAA AGCCGCTGAA AATCAAGTCA AATTCTCTGT CGATCTTGTT 
    AATCAAACTA TAACTTATGG TGATAAACAA GTCAAATTTG ATGTTGAGCC TTTCCGTAAA 
    CATTGTTTGG TTAATGGTTT GGATGACATC GGACTGACTT TGCAAAAAGA AACTATGATT 
    GACGCTTTTG AGGCTGCTAG AGAAGAAAAC TTCCCTTGGA TGAATATCAA GCGCAGCCGG 
    GCTCGCCTTT CACCTGTTAA AAGCAATAAG CAATCTTCTA GCAGGAACGA TTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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