LOC103966454 cDNA ORF clone, Pyrus x bretschneideri(Chinese white pear)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103966454
Entrez Gene ID	103966454
Full Name	phospholipase D beta 1-like
Gene Type	protein-coding
Organism	Pyrus x bretschneideri(Chinese white pear)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009379621.2	XP_009377896.1	phospholipase D beta 1-like isoform X1
XM_009379622.2	XP_009377897.1	phospholipase D beta 1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC103966454 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103966454 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPy82311	XM_009379621.2 Latest version!	Pyrus x bretschneideri phospholipase D beta 1-like (LOC103966454), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPy82312	XM_009379622.2 Latest version!	Pyrus x bretschneideri phospholipase D beta 1-like (LOC103966454), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103966454 ( XM_009379621.2 ) cDNA ORF clone, Pyrus x bretschneideri(Chinese white pear) -> XP_009377896.1 Pyrus x bretschneideri phospholipase D beta 1-like (LOC103966454), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPy82311
Clone ID Related Accession (Same CDS sequence)	XM_009379621.2
Accession Version	XM_009379621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-11
Organism	Pyrus x bretschneideri(Chinese white pear)
Product	phospholipase D beta 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008988353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 12, 2016 this sequence version replaced XM_009379621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pyrus x bretschneideri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTATC CCGCGTACAC CTCGCTATCG TTTGGAGGTT CAGAGCATGG GCAGGGCCAA 
CAAGTTTTGC CATTCAAGAC TGCCAAGGGG TCTCTCAAGG TCTGTCTTTT ACATGGCAAC 
TTAGTTATAT GGGTAATGGA GGCCAGAAAC CTTCCTAACA TGGACATGGT TCATAGGACT 
CTCGGCGACA TGTTTGCTAA ATTTCCAGGG AAGTTTGGCA GCAAGACCGA AGGGCAAGCG 
CCGCAGAAGA TAACCAGTGA TCCCTATGTG ACAATTTCTG TGTCAGGTGC TGTGGTTGGG 
AGGACGTTTG TGATTAGTAA TAGCGAAAAC CCGGTTTGGG GGCAGCATTT TGATGTTCCG 
GTCGCGCATT ATGCTTCGGA GGTGCAGTTT GTTGTGAAAG ATAGTGATGT TGTGGGATCA 
CAGATCATGG GTGCTGTGGG AATCCCAGTT GAGCAATTAC TTTCTGGCAT GAAAGTAGAG 
GGAACATTTC CGATACTTAA TTCGAGCGGG AAAGCGTGTA AGCCTGGAGC CGTTTTGAGC 
TTGTCAATTC AGTATATCCC AATTGAAAAA CTGACATTTT ATGATCAGGG AGTAGGTTCG 
GATCCTGATC ATCAAGGCGT CCCCGGCACT TACTTTCCTC TTAGGAGAGG CGGTAAGGTG 
ACTCTGTATC AAGATGCACA TGTTCATGAT GGTCGCCTTC CGAATTTGGA GCTTGACGGC 
GGTGTTCAGT ATGAGCAAGG GAATTGTTGG CAAGACATGT TCGATGCAAT AAGTCAGGCC 
CGTCGCTTGA TATACATTGC AGGGTGGTCT GTGTACCACA GAGTTAGATT GGTTCGGGAT 
AAGAATGATG CCGCGGATTA CATGTTAGGT GATCTTCTTA AGGCCAAATC ACAGGAAGGT 
GTGAGAGTGC TGCTTCTTGT TTGGGATGAT CCAACTTCCC GGAGAATTTT GGGATATAAA 
ATGGAAGGGG TCATGCAAAC CTCTGACGAG GAAACTCGGC GTTTCTTCAA GCACTCTTCG 
GTCCAAGTGC TGCTTTGCCC TCGATCTGTT GGGAAGGGTA GCTGGGTCAA AAAGCAGGAA 
GCCGAAACTA TCTATACCCA TCATCAGAAA ACAGTGCTTG TGGATGCTGA TGCAGGCAAT 
TCTAAAAGAA AGATCATTGC ATTTGTTGGA GGTCTTGATC TATGCCTGGG GCGCTATGAC 
ACTCCAGATC ATCCAATCTT TAAGAGTTTA CAGACAGTGC ACAAAGATGA CTGTCGTAAC 
CCTACTTTCT CGGAGCGCTC TGTTGGTTGT CCAAGGCAGC CATGGCATGA TTTGCACAGT 
AAAATTGATG GTCCAGCAGC ATATGACATC CTTGCCAATT TTGAGGAGCG TTGGTTGAAA 
GCTTCAAAAC CCCGCGGGCT TCAAAAAATT AAAACACCGC ATGATGATGC ACTACTAAGG 
ATTGAGAGAA TTCCTGAAAT TATTGGGATG GCGGAAGTTC CTTGCCTGAG TGGCGATGAT 
CCAGAAGCTT GGGATGTTCA GGTCTTCCGT TCAATTGATT CCAATTCAGT GAAAGGATTC 
CCGAAGGACT CAAAAGATGC TACAAGCAAG AACCTGGTTT GTGGGAAGAA TGTGCTGATA 
GACATGAGTA TACATGCAGC GTATGTCAAG GCAATCCGTG CGGCTCAACA TTTCATTTAC 
ATAGAAAACC AGTACTTCCT TGGGTCATCA TATAATTGGA ACTTATACAG TGATTTAGGT 
GCAAACAATT TAATACCAAT GGAAATTGCT CTTAAAATCG TAAACAAAAT CAAAGCAAAT 
GAGAGGTTTT CTGTATATAT TGTTATCCCC ATGTGGCCAG AAGGTGTTCC TTCAAGCACT 
CCTATTCAAA GAATTCTCTT TTGGCAGAAT AAAACAATGC AACTGATGTA CGAAATGATT 
TACAAGGCTC TAAAAGAGGC TGGACTTGAA AACAAGTATT GTCCACAAGA CTATTTGAAT 
TTCTTTTGCC TCGGGAATCG CGAAATACCG AATGGTGAAG AAACTTGTGC TGCAAACAAA 
AAAGAAGCAA ATACTCCCCT GGCGCTCACT CAGAAGAATC GGCGTTTTAT GATTTATGTC 
CATTCTAAAG GAATGATTGT GGATGATGAA TATGTTATAA TAGGATCTGC AAATATCAAT 
CAGCGATCCT TGGAAGGTAC CCGGGATACC GAAATAGCAA TGGGAGCATA CCAGCCTCAT 
CACACCTGGG CTAGAAAACT CTCTAGTCCA CATGGAAAGA TATATGGATA TAGAATGTCA 
CTTTGGGCAG AGCATACAGG ATGTCTTGAA GAATGTTTCA AGAAGCCGGC GAGTCTTGAA 
TGCGTGAGAC GGGTGCGATC CTTGAGCGAG CAGAACTGGA GGCAATACGT TGCGGATGAG 
ATTACAGAAA TGAAGGCTCA CCTCCTGAAA TATCCGGTTG AAGTCGATCA AATGGGCAAG 
GTGAAGGCTA TTCCGGGCTG CGAAACTTTT CCAGACGTCG GCGGCAACAT ACTGGGGGCA 
TTCAAAGCCA TCCAAGAAAA TCTCACCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009377896.1
CDS	464..3016
Translation	MAYPAYTSLSFGGSEHGQGQQVLPFKTAKGSLKVCLLHGNLVIWVMEARNLPNMDMVHRTLGDMFAKFPGKFGSKTEGQAPQKITSDPYVTISVSGAVVGRTFVISNSENPVWGQHFDVPVAHYASEVQFVVKDSDVVGSQIMGAVGIPVEQLLSGMKVEGTFPILNSSGKACKPGAVLSLSIQYIPIEKLTFYDQGVGSDPDHQGVPGTYFPLRRGGKVTLYQDAHVHDGRLPNLELDGGVQYEQGNCWQDMFDAISQARRLIYIAGWSVYHRVRLVRDKNDAADYMLGDLLKAKSQEGVRVLLLVWDDPTSRRILGYKMEGVMQTSDEETRRFFKHSSVQVLLCPRSVGKGSWVKKQEAETIYTHHQKTVLVDADAGNSKRKIIAFVGGLDLCLGRYDTPDHPIFKSLQTVHKDDCRNPTFSERSVGCPRQPWHDLHSKIDGPAAYDILANFEERWLKASKPRGLQKIKTPHDDALLRIERIPEIIGMAEVPCLSGDDPEAWDVQVFRSIDSNSVKGFPKDSKDATSKNLVCGKNVLIDMSIHAAYVKAIRAAQHFIYIENQYFLGSSYNWNLYSDLGANNLIPMEIALKIVNKIKANERFSVYIVIPMWPEGVPSSTPIQRILFWQNKTMQLMYEMIYKALKEAGLENKYCPQDYLNFFCLGNREIPNGEETCAANKKEANTPLALTQKNRRFMIYVHSKGMIVDDEYVIIGSANINQRSLEGTRDTEIAMGAYQPHHTWARKLSSPHGKIYGYRMSLWAEHTGCLEECFKKPASLECVRRVRSLSEQNWRQYVADEITEMKAHLLKYPVEVDQMGKVKAIPGCETFPDVGGNILGAFKAIQENLTI

Target ORF information:

RefSeq Version	XM_009379621.2
Organism	Pyrus x bretschneideri(Chinese white pear)
Definition	Pyrus x bretschneideri phospholipase D beta 1-like (LOC103966454), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009379621.2

ORF Insert Sequence:

ATGGCGTATC CCGCGTACAC CTCGCTATCG TTTGGAGGTT CAGAGCATGG GCAGGGCCAA 
CAAGTTTTGC CATTCAAGAC TGCCAAGGGG TCTCTCAAGG TCTGTCTTTT ACATGGCAAC 
TTAGTTATAT GGGTAATGGA GGCCAGAAAC CTTCCTAACA TGGACATGGT TCATAGGACT 
CTCGGCGACA TGTTTGCTAA ATTTCCAGGG AAGTTTGGCA GCAAGACCGA AGGGCAAGCG 
CCGCAGAAGA TAACCAGTGA TCCCTATGTG ACAATTTCTG TGTCAGGTGC TGTGGTTGGG 
AGGACGTTTG TGATTAGTAA TAGCGAAAAC CCGGTTTGGG GGCAGCATTT TGATGTTCCG 
GTCGCGCATT ATGCTTCGGA GGTGCAGTTT GTTGTGAAAG ATAGTGATGT TGTGGGATCA 
CAGATCATGG GTGCTGTGGG AATCCCAGTT GAGCAATTAC TTTCTGGCAT GAAAGTAGAG 
GGAACATTTC CGATACTTAA TTCGAGCGGG AAAGCGTGTA AGCCTGGAGC CGTTTTGAGC 
TTGTCAATTC AGTATATCCC AATTGAAAAA CTGACATTTT ATGATCAGGG AGTAGGTTCG 
GATCCTGATC ATCAAGGCGT CCCCGGCACT TACTTTCCTC TTAGGAGAGG CGGTAAGGTG 
ACTCTGTATC AAGATGCACA TGTTCATGAT GGTCGCCTTC CGAATTTGGA GCTTGACGGC 
GGTGTTCAGT ATGAGCAAGG GAATTGTTGG CAAGACATGT TCGATGCAAT AAGTCAGGCC 
CGTCGCTTGA TATACATTGC AGGGTGGTCT GTGTACCACA GAGTTAGATT GGTTCGGGAT 
AAGAATGATG CCGCGGATTA CATGTTAGGT GATCTTCTTA AGGCCAAATC ACAGGAAGGT 
GTGAGAGTGC TGCTTCTTGT TTGGGATGAT CCAACTTCCC GGAGAATTTT GGGATATAAA 
ATGGAAGGGG TCATGCAAAC CTCTGACGAG GAAACTCGGC GTTTCTTCAA GCACTCTTCG 
GTCCAAGTGC TGCTTTGCCC TCGATCTGTT GGGAAGGGTA GCTGGGTCAA AAAGCAGGAA 
GCCGAAACTA TCTATACCCA TCATCAGAAA ACAGTGCTTG TGGATGCTGA TGCAGGCAAT 
TCTAAAAGAA AGATCATTGC ATTTGTTGGA GGTCTTGATC TATGCCTGGG GCGCTATGAC 
ACTCCAGATC ATCCAATCTT TAAGAGTTTA CAGACAGTGC ACAAAGATGA CTGTCGTAAC 
CCTACTTTCT CGGAGCGCTC TGTTGGTTGT CCAAGGCAGC CATGGCATGA TTTGCACAGT 
AAAATTGATG GTCCAGCAGC ATATGACATC CTTGCCAATT TTGAGGAGCG TTGGTTGAAA 
GCTTCAAAAC CCCGCGGGCT TCAAAAAATT AAAACACCGC ATGATGATGC ACTACTAAGG 
ATTGAGAGAA TTCCTGAAAT TATTGGGATG GCGGAAGTTC CTTGCCTGAG TGGCGATGAT 
CCAGAAGCTT GGGATGTTCA GGTCTTCCGT TCAATTGATT CCAATTCAGT GAAAGGATTC 
CCGAAGGACT CAAAAGATGC TACAAGCAAG AACCTGGTTT GTGGGAAGAA TGTGCTGATA 
GACATGAGTA TACATGCAGC GTATGTCAAG GCAATCCGTG CGGCTCAACA TTTCATTTAC 
ATAGAAAACC AGTACTTCCT TGGGTCATCA TATAATTGGA ACTTATACAG TGATTTAGGT 
GCAAACAATT TAATACCAAT GGAAATTGCT CTTAAAATCG TAAACAAAAT CAAAGCAAAT 
GAGAGGTTTT CTGTATATAT TGTTATCCCC ATGTGGCCAG AAGGTGTTCC TTCAAGCACT 
CCTATTCAAA GAATTCTCTT TTGGCAGAAT AAAACAATGC AACTGATGTA CGAAATGATT 
TACAAGGCTC TAAAAGAGGC TGGACTTGAA AACAAGTATT GTCCACAAGA CTATTTGAAT 
TTCTTTTGCC TCGGGAATCG CGAAATACCG AATGGTGAAG AAACTTGTGC TGCAAACAAA 
AAAGAAGCAA ATACTCCCCT GGCGCTCACT CAGAAGAATC GGCGTTTTAT GATTTATGTC 
CATTCTAAAG GAATGATTGT GGATGATGAA TATGTTATAA TAGGATCTGC AAATATCAAT 
CAGCGATCCT TGGAAGGTAC CCGGGATACC GAAATAGCAA TGGGAGCATA CCAGCCTCAT 
CACACCTGGG CTAGAAAACT CTCTAGTCCA CATGGAAAGA TATATGGATA TAGAATGTCA 
CTTTGGGCAG AGCATACAGG ATGTCTTGAA GAATGTTTCA AGAAGCCGGC GAGTCTTGAA 
TGCGTGAGAC GGGTGCGATC CTTGAGCGAG CAGAACTGGA GGCAATACGT TGCGGATGAG 
ATTACAGAAA TGAAGGCTCA CCTCCTGAAA TATCCGGTTG AAGTCGATCA AATGGGCAAG 
GTGAAGGCTA TTCCGGGCTG CGAAACTTTT CCAGACGTCG GCGGCAACAT ACTGGGGGCA 
TTCAAAGCCA TCCAAGAAAA TCTCACCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103966454 ( XM_009379622.2 ) cDNA ORF clone, Pyrus x bretschneideri(Chinese white pear) -> XP_009377897.1 Pyrus x bretschneideri phospholipase D beta 1-like (LOC103966454), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPy82312
Clone ID Related Accession (Same CDS sequence)	XM_009379622.2
Accession Version	XM_009379622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-11
Organism	Pyrus x bretschneideri(Chinese white pear)
Product	phospholipase D beta 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008988353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 12, 2016 this sequence version replaced XM_009379622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pyrus x bretschneideri Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGGG CCAACAAGTT TTGCCATTCA AGACTGCCAA GGGGTCTCTC AAGGACTCTC 
GGCGACATGT TTGCTAAATT TCCAGGGAAG TTTGGCAGCA AGACCGAAGG GCAAGCGCCG 
CAGAAGATAA CCAGTGATCC CTATGTGACA ATTTCTGTGT CAGGTGCTGT GGTTGGGAGG 
ACGTTTGTGA TTAGTAATAG CGAAAACCCG GTTTGGGGGC AGCATTTTGA TGTTCCGGTC 
GCGCATTATG CTTCGGAGGT GCAGTTTGTT GTGAAAGATA GTGATGTTGT GGGATCACAG 
ATCATGGGTG CTGTGGGAAT CCCAGTTGAG CAATTACTTT CTGGCATGAA AGTAGAGGGA 
ACATTTCCGA TACTTAATTC GAGCGGGAAA GCGTGTAAGC CTGGAGCCGT TTTGAGCTTG 
TCAATTCAGT ATATCCCAAT TGAAAAACTG ACATTTTATG ATCAGGGAGT AGGTTCGGAT 
CCTGATCATC AAGGCGTCCC CGGCACTTAC TTTCCTCTTA GGAGAGGCGG TAAGGTGACT 
CTGTATCAAG ATGCACATGT TCATGATGGT CGCCTTCCGA ATTTGGAGCT TGACGGCGGT 
GTTCAGTATG AGCAAGGGAA TTGTTGGCAA GACATGTTCG ATGCAATAAG TCAGGCCCGT 
CGCTTGATAT ACATTGCAGG GTGGTCTGTG TACCACAGAG TTAGATTGGT TCGGGATAAG 
AATGATGCCG CGGATTACAT GTTAGGTGAT CTTCTTAAGG CCAAATCACA GGAAGGTGTG 
AGAGTGCTGC TTCTTGTTTG GGATGATCCA ACTTCCCGGA GAATTTTGGG ATATAAAATG 
GAAGGGGTCA TGCAAACCTC TGACGAGGAA ACTCGGCGTT TCTTCAAGCA CTCTTCGGTC 
CAAGTGCTGC TTTGCCCTCG ATCTGTTGGG AAGGGTAGCT GGGTCAAAAA GCAGGAAGCC 
GAAACTATCT ATACCCATCA TCAGAAAACA GTGCTTGTGG ATGCTGATGC AGGCAATTCT 
AAAAGAAAGA TCATTGCATT TGTTGGAGGT CTTGATCTAT GCCTGGGGCG CTATGACACT 
CCAGATCATC CAATCTTTAA GAGTTTACAG ACAGTGCACA AAGATGACTG TCGTAACCCT 
ACTTTCTCGG AGCGCTCTGT TGGTTGTCCA AGGCAGCCAT GGCATGATTT GCACAGTAAA 
ATTGATGGTC CAGCAGCATA TGACATCCTT GCCAATTTTG AGGAGCGTTG GTTGAAAGCT 
TCAAAACCCC GCGGGCTTCA AAAAATTAAA ACACCGCATG ATGATGCACT ACTAAGGATT 
GAGAGAATTC CTGAAATTAT TGGGATGGCG GAAGTTCCTT GCCTGAGTGG CGATGATCCA 
GAAGCTTGGG ATGTTCAGGT CTTCCGTTCA ATTGATTCCA ATTCAGTGAA AGGATTCCCG 
AAGGACTCAA AAGATGCTAC AAGCAAGAAC CTGGTTTGTG GGAAGAATGT GCTGATAGAC 
ATGAGTATAC ATGCAGCGTA TGTCAAGGCA ATCCGTGCGG CTCAACATTT CATTTACATA 
GAAAACCAGT ACTTCCTTGG GTCATCATAT AATTGGAACT TATACAGTGA TTTAGGTGCA 
AACAATTTAA TACCAATGGA AATTGCTCTT AAAATCGTAA ACAAAATCAA AGCAAATGAG 
AGGTTTTCTG TATATATTGT TATCCCCATG TGGCCAGAAG GTGTTCCTTC AAGCACTCCT 
ATTCAAAGAA TTCTCTTTTG GCAGAATAAA ACAATGCAAC TGATGTACGA AATGATTTAC 
AAGGCTCTAA AAGAGGCTGG ACTTGAAAAC AAGTATTGTC CACAAGACTA TTTGAATTTC 
TTTTGCCTCG GGAATCGCGA AATACCGAAT GGTGAAGAAA CTTGTGCTGC AAACAAAAAA 
GAAGCAAATA CTCCCCTGGC GCTCACTCAG AAGAATCGGC GTTTTATGAT TTATGTCCAT 
TCTAAAGGAA TGATTGTGGA TGATGAATAT GTTATAATAG GATCTGCAAA TATCAATCAG 
CGATCCTTGG AAGGTACCCG GGATACCGAA ATAGCAATGG GAGCATACCA GCCTCATCAC 
ACCTGGGCTA GAAAACTCTC TAGTCCACAT GGAAAGATAT ATGGATATAG AATGTCACTT 
TGGGCAGAGC ATACAGGATG TCTTGAAGAA TGTTTCAAGA AGCCGGCGAG TCTTGAATGC 
GTGAGACGGG TGCGATCCTT GAGCGAGCAG AACTGGAGGC AATACGTTGC GGATGAGATT 
ACAGAAATGA AGGCTCACCT CCTGAAATAT CCGGTTGAAG TCGATCAAAT GGGCAAGGTG 
AAGGCTATTC CGGGCTGCGA AACTTTTCCA GACGTCGGCG GCAACATACT GGGGGCATTC 
AAAGCCATCC AAGAAAATCT CACCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009377897.1
CDS	509..2938
Translation	MGRANKFCHSRLPRGLSRTLGDMFAKFPGKFGSKTEGQAPQKITSDPYVTISVSGAVVGRTFVISNSENPVWGQHFDVPVAHYASEVQFVVKDSDVVGSQIMGAVGIPVEQLLSGMKVEGTFPILNSSGKACKPGAVLSLSIQYIPIEKLTFYDQGVGSDPDHQGVPGTYFPLRRGGKVTLYQDAHVHDGRLPNLELDGGVQYEQGNCWQDMFDAISQARRLIYIAGWSVYHRVRLVRDKNDAADYMLGDLLKAKSQEGVRVLLLVWDDPTSRRILGYKMEGVMQTSDEETRRFFKHSSVQVLLCPRSVGKGSWVKKQEAETIYTHHQKTVLVDADAGNSKRKIIAFVGGLDLCLGRYDTPDHPIFKSLQTVHKDDCRNPTFSERSVGCPRQPWHDLHSKIDGPAAYDILANFEERWLKASKPRGLQKIKTPHDDALLRIERIPEIIGMAEVPCLSGDDPEAWDVQVFRSIDSNSVKGFPKDSKDATSKNLVCGKNVLIDMSIHAAYVKAIRAAQHFIYIENQYFLGSSYNWNLYSDLGANNLIPMEIALKIVNKIKANERFSVYIVIPMWPEGVPSSTPIQRILFWQNKTMQLMYEMIYKALKEAGLENKYCPQDYLNFFCLGNREIPNGEETCAANKKEANTPLALTQKNRRFMIYVHSKGMIVDDEYVIIGSANINQRSLEGTRDTEIAMGAYQPHHTWARKLSSPHGKIYGYRMSLWAEHTGCLEECFKKPASLECVRRVRSLSEQNWRQYVADEITEMKAHLLKYPVEVDQMGKVKAIPGCETFPDVGGNILGAFKAIQENLTI

Target ORF information:

RefSeq Version	XM_009379622.2
Organism	Pyrus x bretschneideri(Chinese white pear)
Definition	Pyrus x bretschneideri phospholipase D beta 1-like (LOC103966454), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009379622.2

ORF Insert Sequence:

ATGGGCAGGG CCAACAAGTT TTGCCATTCA AGACTGCCAA GGGGTCTCTC AAGGACTCTC 
GGCGACATGT TTGCTAAATT TCCAGGGAAG TTTGGCAGCA AGACCGAAGG GCAAGCGCCG 
CAGAAGATAA CCAGTGATCC CTATGTGACA ATTTCTGTGT CAGGTGCTGT GGTTGGGAGG 
ACGTTTGTGA TTAGTAATAG CGAAAACCCG GTTTGGGGGC AGCATTTTGA TGTTCCGGTC 
GCGCATTATG CTTCGGAGGT GCAGTTTGTT GTGAAAGATA GTGATGTTGT GGGATCACAG 
ATCATGGGTG CTGTGGGAAT CCCAGTTGAG CAATTACTTT CTGGCATGAA AGTAGAGGGA 
ACATTTCCGA TACTTAATTC GAGCGGGAAA GCGTGTAAGC CTGGAGCCGT TTTGAGCTTG 
TCAATTCAGT ATATCCCAAT TGAAAAACTG ACATTTTATG ATCAGGGAGT AGGTTCGGAT 
CCTGATCATC AAGGCGTCCC CGGCACTTAC TTTCCTCTTA GGAGAGGCGG TAAGGTGACT 
CTGTATCAAG ATGCACATGT TCATGATGGT CGCCTTCCGA ATTTGGAGCT TGACGGCGGT 
GTTCAGTATG AGCAAGGGAA TTGTTGGCAA GACATGTTCG ATGCAATAAG TCAGGCCCGT 
CGCTTGATAT ACATTGCAGG GTGGTCTGTG TACCACAGAG TTAGATTGGT TCGGGATAAG 
AATGATGCCG CGGATTACAT GTTAGGTGAT CTTCTTAAGG CCAAATCACA GGAAGGTGTG 
AGAGTGCTGC TTCTTGTTTG GGATGATCCA ACTTCCCGGA GAATTTTGGG ATATAAAATG 
GAAGGGGTCA TGCAAACCTC TGACGAGGAA ACTCGGCGTT TCTTCAAGCA CTCTTCGGTC 
CAAGTGCTGC TTTGCCCTCG ATCTGTTGGG AAGGGTAGCT GGGTCAAAAA GCAGGAAGCC 
GAAACTATCT ATACCCATCA TCAGAAAACA GTGCTTGTGG ATGCTGATGC AGGCAATTCT 
AAAAGAAAGA TCATTGCATT TGTTGGAGGT CTTGATCTAT GCCTGGGGCG CTATGACACT 
CCAGATCATC CAATCTTTAA GAGTTTACAG ACAGTGCACA AAGATGACTG TCGTAACCCT 
ACTTTCTCGG AGCGCTCTGT TGGTTGTCCA AGGCAGCCAT GGCATGATTT GCACAGTAAA 
ATTGATGGTC CAGCAGCATA TGACATCCTT GCCAATTTTG AGGAGCGTTG GTTGAAAGCT 
TCAAAACCCC GCGGGCTTCA AAAAATTAAA ACACCGCATG ATGATGCACT ACTAAGGATT 
GAGAGAATTC CTGAAATTAT TGGGATGGCG GAAGTTCCTT GCCTGAGTGG CGATGATCCA 
GAAGCTTGGG ATGTTCAGGT CTTCCGTTCA ATTGATTCCA ATTCAGTGAA AGGATTCCCG 
AAGGACTCAA AAGATGCTAC AAGCAAGAAC CTGGTTTGTG GGAAGAATGT GCTGATAGAC 
ATGAGTATAC ATGCAGCGTA TGTCAAGGCA ATCCGTGCGG CTCAACATTT CATTTACATA 
GAAAACCAGT ACTTCCTTGG GTCATCATAT AATTGGAACT TATACAGTGA TTTAGGTGCA 
AACAATTTAA TACCAATGGA AATTGCTCTT AAAATCGTAA ACAAAATCAA AGCAAATGAG 
AGGTTTTCTG TATATATTGT TATCCCCATG TGGCCAGAAG GTGTTCCTTC AAGCACTCCT 
ATTCAAAGAA TTCTCTTTTG GCAGAATAAA ACAATGCAAC TGATGTACGA AATGATTTAC 
AAGGCTCTAA AAGAGGCTGG ACTTGAAAAC AAGTATTGTC CACAAGACTA TTTGAATTTC 
TTTTGCCTCG GGAATCGCGA AATACCGAAT GGTGAAGAAA CTTGTGCTGC AAACAAAAAA 
GAAGCAAATA CTCCCCTGGC GCTCACTCAG AAGAATCGGC GTTTTATGAT TTATGTCCAT 
TCTAAAGGAA TGATTGTGGA TGATGAATAT GTTATAATAG GATCTGCAAA TATCAATCAG 
CGATCCTTGG AAGGTACCCG GGATACCGAA ATAGCAATGG GAGCATACCA GCCTCATCAC 
ACCTGGGCTA GAAAACTCTC TAGTCCACAT GGAAAGATAT ATGGATATAG AATGTCACTT 
TGGGCAGAGC ATACAGGATG TCTTGAAGAA TGTTTCAAGA AGCCGGCGAG TCTTGAATGC 
GTGAGACGGG TGCGATCCTT GAGCGAGCAG AACTGGAGGC AATACGTTGC GGATGAGATT 
ACAGAAATGA AGGCTCACCT CCTGAAATAT CCGGTTGAAG TCGATCAAAT GGGCAAGGTG 
AAGGCTATTC CGGGCTGCGA AACTTTTCCA GACGTCGGCG GCAACATACT GGGGGCATTC 
AAAGCCATCC AAGAAAATCT CACCATCTGA