SDS-PAGE analysis of protein purified by Anti-His Affinity Resin or Ni-NTA Agarose.
Test result demonstrated that the Anti-His Affinity Resin can purify the His-tagged proteins with high purity from
different samples in which Ni-NTA Agarose had failed.

SDS-PAGE analysis of protein eluted from Anti-His Affinity Resin.
M. Marker
1. His-tagged protein using the acid elution procedure
2. His-tagged protein using the alkaline elution procedure
3. His-tagged protein by competitive elution procedure
Test result demonstrated that the His-tagged protein can be eluted by three different eluents.

Anti-His Affinity Resin

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THETM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.

Cat. No.	L00439
Size	1 ml Resin (Total 2 ml) : Quote 5 ml Resin (Total 10 ml) : Quote Buy in bulk
Quantity
Price	¥57,540.00


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Description

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THE^TM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.

Note

Note:

The product is designed for purification and immunoprecipitation of His-tagged protein, especially for the sample failed by Ni-NTA Agarose Chromatography purification.

Overview

Key Features

Characteristics:

Features	Specifications
Beads structure	4% agarose
Beads size	45-165 μm, averagely 90 μm
Coupled antibody	THE^TM His Tag Antibody, mAb, Mouse (Cat.No A00186)
Binding capacity	More than 0.6 mg of His-tagged protein per mL settled resin
Specificity	N-terminal/C-terminal/internal/4 x His/5 x His/6 x His-tagged protein
Stability	Stable at 2-8 °C for 1 year
Storage	2-8 °C. Avoid freezing the resin
Repeat usage	Generally the resin can be recycled at least 4 times. If maintained properly, the resin may be recycled 10 times with minor binding capability loss
Elution methods	Acid, alkaline and peptide competitive elutions
Reagents compatibility	Compatible with mostly used reagents at certain concentrations
Consistency	Lot-to-lot consistency proved by strict production procedure

Cat. No.	Product Name	Volume supplied	Resin content
L00439-1	Anti-His Affinity Resin 1 ml	2 ml	50% slurry
L00439-5	Anti-His Affinity Resin 5 ml	10 ml	50% slurry

Examples