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Protein A Resin FF Prepacked Column

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
L00680
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Description

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.

_x000D_ Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

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Cat. No.Product Name_x000D_ Volume supplied_x000D_ Resin content_x000D_
L00680-12Protein A Resin FF (1mlx2)1mlx250% slurry
L00680-51Protein A Resin FF (5mlx1)5mlx150% slurry
L00680-55Protein A Resin FF (5mlx5)5mlx550% slurry
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Characteristics
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LigandRecombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand5
M.W. of ligandApproximately 34 kDa
PI of ligand5.17
Degree of substitutionApproximately 5 mg protein A / ml settled resin
Static binding capacity> 40 mg human IgG/ ml settled resin
Matrix componentAgarose, 4% highly cross-linked
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol
Storage conditions2-8 °C
Shelf life18 months when stored unopened
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_x000D_ Column compatibility_x000D_
_x000D_ The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa). _x000D_

Properties
Storage & Stability Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.