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Protein A Resin

Protein A Resin

GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein A Resin.
L00210
¥15,092.00

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Description

GenScript Protein A Resin is an affinity_x000D_ chromatography medium designed for easy, one-step purification of classes, subclasses_x000D_ and fragments of immunoglobulins from biological fluids and from cell culture_x000D_ media. Protein A Resin can also be used for immunoprecipitation of_x000D_ proteins, protein complexes or antigens. The recombinant protein A ligand is_x000D_ coupled to 4%_x000D_ agarose. The coupling is optimized to give high binding capacity for_x000D_ immunoglobulins. The static binding_x000D_ capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin._x000D_ The binding capacity will_x000D_ vary depending on several factors such as target antibody, flow rate etc. Table_x000D_ 1 lists the characteristics of Protein A Resin.

_x000D_ _x000D_ Protein A, a bacterial cell wall protein isolated_x000D_ from Staphylococcus_x000D_ aureus,_x000D_ binds to mammalian IgGs mainly through Fc regions. Native_x000D_ protein A has five IgG binding domains and many other domains with unknown_x000D_ functions. Recombinant protein A contains five high_x000D_ affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region_x000D_ is involved in binding, the Fab region is available for binding antigens.
Note

High temperature heating is not recommended. The agarose melts above 65°C.

Overview
Product Information Characteristics of Protein A Resin
Resin Volume5ml settled resin (10 ml 50% slurry)
LigandRecombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand5
M.W. of ligandApproximately 34 kDa
PI of ligand5.17
Degree of substitutionApproximately 2 mg protein A/ml settled resin
Static binding capacity
> 20 mg human IgG/ml settled resin
Matrix sphericalAgarose, 4% 
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol 
Storage conditions2-8 °C;  Do not freeze

Properties
Storage & Stability Store regenerated Protein A Resin in 20% ethanol at 2°C to 8°C. Do not freeze.

Examples
  • Protein A Resin
    • Protein A Resin