Protein Isolation and Analysis » タンパク質単離・精製 » 精製用レジン » High Affinity Ni-Charged Resin FF Prepacked Column

High Affinity Ni-Charged Resin FF Prepacked Column

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins.
L00683
¥9,660.00

Ask us a question
Description

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.
_x000D_

_x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Cat. No.Product Name_x000D_ Volume supplied_x000D_ Resin content_x000D_
L00683-12High Affinity Ni-charged Resin FF prepacked column (1mlx2)1mlx2prepacked column
L00683-51High Affinity Ni-charged Resin FF prepacked column (5mlx1)5mlx1
L00683-55High Affinity Ni-charged Resin FF prepacked column (5mlx5)5mlx5
_x000D_
_x000D_ _x000D_ Storage_x000D_ Store in 20% ethanol at 2°C to 8°C. Do not freeze._x000D_
_x000D_
_x000D_ Characteristics_x000D_
_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Binding capacity>40 mg of histidine-tagged protein /ml settled resin
Matrix spherical6% highly cross-linked agarose
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol
Storage and Stability18 months when stored unopened at 2-8 °C
_x000D_
_x000D_
_x000D_ Column compatibility_x000D_
_x000D_ The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa). _x000D_ _x000D_
_x000D_
_x000D_ _x000D_ Reagents Compatible_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
DenaturantsDetergentsReducing agentsSalts Others
6 M Gu·HCl2% Triton X-100 20 mM β-ME4 M MgCl250% glycerol
8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl220% ethanol
1% CHAPS2 M NaCl1 mM EDTA

Properties
Storage & Stability Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.