usp25 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following usp25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42591 XM_018091320.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa51474 XM_012957169.3
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa51475 XM_031896080.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa23009 NM_001045687.1
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa28753 XM_012957169.2
Latest version!
Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa42591
    Clone ID Related Accession (Same CDS sequence) XM_018091320.1
    Accession Version XM_018091320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCAGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG GTCATGATGA CCCCGAACAT GCAAGGTATA
    ATCATGGCCA TAGGTAAATC CCGGAATGTG TATGACAAGT GTGGCCCTGA AGCAGGGTTC
    TTTAAGGCCA TCAAAACTGA ATACGCTCGG CTAGTGAAAC TAGCGCAAGA AGACCCCCCG
    CCAGAGATAG ATTACCGCTT GCAGCACGCC ATAGTCTACT TCATCCAAAG CCAAGCACCA
    AAGAAAATCA TAGAAAGGAC ATTGCTGGAG CAGTTTGCAG ACCGTAATTT GAGTTTTGAT
    GAACGATGCC GCAATATTAT GAAAGTTGCT CAGGCTAAAC TGGAAATGAT AAAACCAGAT
    GAGGTCAACA TGGAGGAATA CAGGAAGTGG CACAAGGACT ATAGAATGTT CCGAGAGACA
    ACAATGTACA TCTTGATTGG TCTGGAGCTG TTTCAGAACA AGAGCTATGT TGAAGCGCTG
    CTGTACCTAA TTTATGGCTA CCAGTACAAT AAAGAGCTGA TCTCGAAAGG CCTCTACAGA
    GGGCATGATG AGGAGCTCAT ATCCCATTAC AGAAGGGAGT GCTTTCTGAA ACTGAATGAG
    CAGGCAGCAG CCATGTTTGA ATCTGGAGAC GTTGATGAAG TTACTAACGG GCTTACATCC
    ATGAACGAAC TCATCGTCCC ATGTTTGCCC TTGCTGCTGG TCGATGACAT GGAGGAGAAG
    GACATTGTTG CTGTGGAAGA CATGAGAAAC CGTTGGTGTT CCTACCTTGG GCAAGAAATG
    GAGCCAAATC TGCAAGAAAA GCTGACCGAC TTCCTGCCAA AACTGCTTGA TTGTTCAACA
    GAAATCAAAG GTTTAAACGA CCCGCCAAAG CTTCCCAACT TCTCCACGCA CGAACTGTGT
    GAGCGCTTCG CCCGAATCAT GTTGTCACTT AGCCGAATGC CGGCCGACGG GAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946809.1
    CDS375..3611
    Translation

    Target ORF information:

    RefSeq Version XM_018091320.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCAGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG GTCATGATGA CCCCGAACAT GCAAGGTATA
    ATCATGGCCA TAGGTAAATC CCGGAATGTG TATGACAAGT GTGGCCCTGA AGCAGGGTTC
    TTTAAGGCCA TCAAAACTGA ATACGCTCGG CTAGTGAAAC TAGCGCAAGA AGACCCCCCG
    CCAGAGATAG ATTACCGCTT GCAGCACGCC ATAGTCTACT TCATCCAAAG CCAAGCACCA
    AAGAAAATCA TAGAAAGGAC ATTGCTGGAG CAGTTTGCAG ACCGTAATTT GAGTTTTGAT
    GAACGATGCC GCAATATTAT GAAAGTTGCT CAGGCTAAAC TGGAAATGAT AAAACCAGAT
    GAGGTCAACA TGGAGGAATA CAGGAAGTGG CACAAGGACT ATAGAATGTT CCGAGAGACA
    ACAATGTACA TCTTGATTGG TCTGGAGCTG TTTCAGAACA AGAGCTATGT TGAAGCGCTG
    CTGTACCTAA TTTATGGCTA CCAGTACAAT AAAGAGCTGA TCTCGAAAGG CCTCTACAGA
    GGGCATGATG AGGAGCTCAT ATCCCATTAC AGAAGGGAGT GCTTTCTGAA ACTGAATGAG
    CAGGCAGCAG CCATGTTTGA ATCTGGAGAC GTTGATGAAG TTACTAACGG GCTTACATCC
    ATGAACGAAC TCATCGTCCC ATGTTTGCCC TTGCTGCTGG TCGATGACAT GGAGGAGAAG
    GACATTGTTG CTGTGGAAGA CATGAGAAAC CGTTGGTGTT CCTACCTTGG GCAAGAAATG
    GAGCCAAATC TGCAAGAAAA GCTGACCGAC TTCCTGCCAA AACTGCTTGA TTGTTCAACA
    GAAATCAAAG GTTTAAACGA CCCGCCAAAG CTTCCCAACT TCTCCACGCA CGAACTGTGT
    GAGCGCTTCG CCCGAATCAT GTTGTCACTT AGCCGAATGC CGGCCGACGG GAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51474
    Clone ID Related Accession (Same CDS sequence) XM_012957169.3
    Accession Version XM_012957169.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCGGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG CCCACTGATA ACGCCCCAGC TCCAGCAGCT
    CCTAACCAAC GAGTTGTAGA GGTGGCGATC CCTCACGTAG GGACTTTTGT TATTGAGTCA
    GAGGAGGGGG GTTATGACGA TGAGGTCATG ATGACCCCGA ACATGCAAGG TATAATCATG
    GCCATAGGTA AATCCCGGAA TGTGTATGAC AAGTGTGGCC CTGAAGCAGG GTTCTTTAAG
    GCCATCAAAA CTGAATACGC TCGGCTAGTG AAACTAGCGC AAGAAGACCC CCCGCCAGAG
    ATAGATTACC GCTTGCAGCA CGCCATAGTC TACTTCATCC AAAGCCAAGC ACCAAAGAAA
    ATCATAGAAA GGACATTGCT GGAGCAGTTT GCAGACCGTA ATTTGAGTTT TGATGAACGA
    TGCCGCAATA TTATGAAAGT TGCTCAGGCT AAACTGGAAA TGATAAAACC AGATGAGGTC
    AACATGGAGG AATACAGGAA GTGGCACAAG GACTATAGAA TGTTCCGAGA GACAACAATG
    TACATCTTGA TTGGTCTGGA GCTGTTTCAG AACAAGAGCT ATGTTGAAGC GCTGCTGTAC
    CTAATTTATG GCTACCAGTA CAATAAAGAG CTGATCTCGA AAGGCCTCTA CAGAGGGCAT
    GATGAGGAGC TCATATCCCA TTACAGAAGG GAGTGCTTTC TGAAACTGAA TGAGCAGGCA
    GCAGCCATGT TTGAATCTGG AGACGTTGAT GAAGTTACTA ACGGGCTTAC ATCCATGAAC
    GAACTCATCG TCCCATGTTT GCCCTTGCTG CTGGTCGATG ACATGGAGGA GAAGGACATT
    GTTGCTGTGG AAGACATGAG AAACCGTTGG TGTTCCTACC TTGGGCAAGA AATGGAGCCA
    AATCTGCAAG AAAAGCTGAC CGACTTCCTG CCAAAACTGC TTGATTGTTC AACAGAAATC
    AAAGGTTTAA ACGACCCGCC AAAGCTTCCC AACTTCTCCA CGCACGAACT GTGTGAGCGC
    TTCGCCCGAA TCATGTTGTC ACTTAGCCGA ATGCCGGCCG ACGGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812623.1
    CDS370..3720
    Translation

    Target ORF information:

    RefSeq Version XM_012957169.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCGGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG CCCACTGATA ACGCCCCAGC TCCAGCAGCT
    CCTAACCAAC GAGTTGTAGA GGTGGCGATC CCTCACGTAG GGACTTTTGT TATTGAGTCA
    GAGGAGGGGG GTTATGACGA TGAGGTCATG ATGACCCCGA ACATGCAAGG TATAATCATG
    GCCATAGGTA AATCCCGGAA TGTGTATGAC AAGTGTGGCC CTGAAGCAGG GTTCTTTAAG
    GCCATCAAAA CTGAATACGC TCGGCTAGTG AAACTAGCGC AAGAAGACCC CCCGCCAGAG
    ATAGATTACC GCTTGCAGCA CGCCATAGTC TACTTCATCC AAAGCCAAGC ACCAAAGAAA
    ATCATAGAAA GGACATTGCT GGAGCAGTTT GCAGACCGTA ATTTGAGTTT TGATGAACGA
    TGCCGCAATA TTATGAAAGT TGCTCAGGCT AAACTGGAAA TGATAAAACC AGATGAGGTC
    AACATGGAGG AATACAGGAA GTGGCACAAG GACTATAGAA TGTTCCGAGA GACAACAATG
    TACATCTTGA TTGGTCTGGA GCTGTTTCAG AACAAGAGCT ATGTTGAAGC GCTGCTGTAC
    CTAATTTATG GCTACCAGTA CAATAAAGAG CTGATCTCGA AAGGCCTCTA CAGAGGGCAT
    GATGAGGAGC TCATATCCCA TTACAGAAGG GAGTGCTTTC TGAAACTGAA TGAGCAGGCA
    GCAGCCATGT TTGAATCTGG AGACGTTGAT GAAGTTACTA ACGGGCTTAC ATCCATGAAC
    GAACTCATCG TCCCATGTTT GCCCTTGCTG CTGGTCGATG ACATGGAGGA GAAGGACATT
    GTTGCTGTGG AAGACATGAG AAACCGTTGG TGTTCCTACC TTGGGCAAGA AATGGAGCCA
    AATCTGCAAG AAAAGCTGAC CGACTTCCTG CCAAAACTGC TTGATTGTTC AACAGAAATC
    AAAGGTTTAA ACGACCCGCC AAAGCTTCCC AACTTCTCCA CGCACGAACT GTGTGAGCGC
    TTCGCCCGAA TCATGTTGTC ACTTAGCCGA ATGCCGGCCG ACGGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51475
    Clone ID Related Accession (Same CDS sequence) XM_031896080.1
    Accession Version XM_031896080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCGGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG CCCACTGATA ACGCCCCAGC TCCAGCAGCT
    CCTAACCAAC GAGTTGTAGA GGTGGCGATC CCTCACGTAG GGACTTTTGT TATTGAGTCA
    GAGGAGGGGG GTTATGACGA TGAGGCCATC AAAACTGAAT ACGCTCGGCT AGTGAAACTA
    GCGCAAGAAG ACCCCCCGCC AGAGATAGAT TACCGCTTGC AGCACGCCAT AGTCTACTTC
    ATCCAAAGCC AAGCACCAAA GAAAATCATA GAAAGGACAT TGCTGGAGCA GTTTGCAGAC
    CGTAATTTGA GTTTTGATGA ACGATGCCGC AATATTATGA AAGTTGCTCA GGCTAAACTG
    GAAATGATAA AACCAGATGA GGTCAACATG GAGGAATACA GGAAGTGGCA CAAGGACTAT
    AGAATGTTCC GAGAGACAAC AATGTACATC TTGATTGGTC TGGAGCTGTT TCAGAACAAG
    AGCTATGTTG AAGCGCTGCT GTACCTAATT TATGGCTACC AGTACAATAA AGAGCTGATC
    TCGAAAGGCC TCTACAGAGG GCATGATGAG GAGCTCATAT CCCATTACAG AAGGGAGTGC
    TTTCTGAAAC TGAATGAGCA GGCAGCAGCC ATGTTTGAAT CTGGAGACGT TGATGAAGTT
    ACTAACGGGC TTACATCCAT GAACGAACTC ATCGTCCCAT GTTTGCCCTT GCTGCTGGTC
    GATGACATGG AGGAGAAGGA CATTGTTGCT GTGGAAGACA TGAGAAACCG TTGGTGTTCC
    TACCTTGGGC AAGAAATGGA GCCAAATCTG CAAGAAAAGC TGACCGACTT CCTGCCAAAA
    CTGCTTGATT GTTCAACAGA AATCAAAGGT TTAAACGACC CGCCAAAGCT TCCCAACTTC
    TCCACGCACG AACTGTGTGA GCGCTTCGCC CGAATCATGT TGTCACTTAG CCGAATGCCG
    GCCGACGGGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751940.1
    CDS370..3624
    Translation

    Target ORF information:

    RefSeq Version XM_031896080.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCGGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG CCCACTGATA ACGCCCCAGC TCCAGCAGCT
    CCTAACCAAC GAGTTGTAGA GGTGGCGATC CCTCACGTAG GGACTTTTGT TATTGAGTCA
    GAGGAGGGGG GTTATGACGA TGAGGCCATC AAAACTGAAT ACGCTCGGCT AGTGAAACTA
    GCGCAAGAAG ACCCCCCGCC AGAGATAGAT TACCGCTTGC AGCACGCCAT AGTCTACTTC
    ATCCAAAGCC AAGCACCAAA GAAAATCATA GAAAGGACAT TGCTGGAGCA GTTTGCAGAC
    CGTAATTTGA GTTTTGATGA ACGATGCCGC AATATTATGA AAGTTGCTCA GGCTAAACTG
    GAAATGATAA AACCAGATGA GGTCAACATG GAGGAATACA GGAAGTGGCA CAAGGACTAT
    AGAATGTTCC GAGAGACAAC AATGTACATC TTGATTGGTC TGGAGCTGTT TCAGAACAAG
    AGCTATGTTG AAGCGCTGCT GTACCTAATT TATGGCTACC AGTACAATAA AGAGCTGATC
    TCGAAAGGCC TCTACAGAGG GCATGATGAG GAGCTCATAT CCCATTACAG AAGGGAGTGC
    TTTCTGAAAC TGAATGAGCA GGCAGCAGCC ATGTTTGAAT CTGGAGACGT TGATGAAGTT
    ACTAACGGGC TTACATCCAT GAACGAACTC ATCGTCCCAT GTTTGCCCTT GCTGCTGGTC
    GATGACATGG AGGAGAAGGA CATTGTTGCT GTGGAAGACA TGAGAAACCG TTGGTGTTCC
    TACCTTGGGC AAGAAATGGA GCCAAATCTG CAAGAAAAGC TGACCGACTT CCTGCCAAAA
    CTGCTTGATT GTTCAACAGA AATCAAAGGT TTAAACGACC CGCCAAAGCT TCCCAACTTC
    TCCACGCACG AACTGTGTGA GCGCTTCGCC CGAATCATGT TGTCACTTAG CCGAATGCCG
    GCCGACGGGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa23009
    Clone ID Related Accession (Same CDS sequence) NM_001045687.1
    Accession Version NM_001045687.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 25
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR942577.2. ##Evidence-Data-START## Transcript exon combination :: CR942577.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCAGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG GCCATCAAAA CTGAATACGC TCGGCTAGTG
    AAACTAGCGC AAGAAGACCC CCCGCCAGAG ATAGATTACC GCTTGCAGCA CGCCATAGTC
    TACTTCATCC AAAGCCAAGC ACCAAAGAAA ATCATAGAAA GGACATTGCT GGAGCAGTTT
    GCAGACCGTA ATTTGAGTTT TGATGAACGA TGCCGCAATA TTATGAAAGT TGCTCAGGCT
    AAACTGGAAA TGATAAAACC AGATGAGGTC AACATGGAGG AATACAGGAA GTGGCACAAG
    GACTATAGAA TGTTCCGAGA GACAACAATG TACATCTTGA TTGGTCTGGA GCTGTTTCAG
    AACAAGAGCT ATGTTGAAGC GCTGCTGTAC CTAATTTATG GCTACCAGTA CAATAAAGAG
    CTGATCTCGA AAGGCCTCTA CAGAGGGCAT GATGAGGAGC TCATATCCCA TTACAGAAGG
    GAGTGCTTTC TGAAACTGAA TGAGCAGGCA GCAGCCATGT TTGAATCTGG AGACGTTGAT
    GAAGTTACTA ACGGGCTTAC ATCCATGAAC GAACTCATCG TCCCATGTTT GCCCTTGCTG
    CTGGTCGATG ACATGGAGGA GAAGGACATT GTTGCTGTGG AAGACATGAG AAACCGTTGG
    TGTTCCTACC TTGGGCAAGA AATGGAGCCA AATCTGCAAG AAAAGCTGAC CGACTTCCTG
    CCAAAACTGC TTGATTGTTC AACAGAAATC AAAGGTTTAA ACGACCCGCC AAAGCTTCCC
    AACTTCTCCA CGCACGAACT GTGTGAGCGC TTCGCCCGAA TCATGTTGTC ACTTAGCCGA
    ATGCCGGCCG ACGGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001039152.1
    CDS258..3398
    Translation

    Target ORF information:

    RefSeq Version NM_001045687.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045687.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCAGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG GCCATCAAAA CTGAATACGC TCGGCTAGTG
    AAACTAGCGC AAGAAGACCC CCCGCCAGAG ATAGATTACC GCTTGCAGCA CGCCATAGTC
    TACTTCATCC AAAGCCAAGC ACCAAAGAAA ATCATAGAAA GGACATTGCT GGAGCAGTTT
    GCAGACCGTA ATTTGAGTTT TGATGAACGA TGCCGCAATA TTATGAAAGT TGCTCAGGCT
    AAACTGGAAA TGATAAAACC AGATGAGGTC AACATGGAGG AATACAGGAA GTGGCACAAG
    GACTATAGAA TGTTCCGAGA GACAACAATG TACATCTTGA TTGGTCTGGA GCTGTTTCAG
    AACAAGAGCT ATGTTGAAGC GCTGCTGTAC CTAATTTATG GCTACCAGTA CAATAAAGAG
    CTGATCTCGA AAGGCCTCTA CAGAGGGCAT GATGAGGAGC TCATATCCCA TTACAGAAGG
    GAGTGCTTTC TGAAACTGAA TGAGCAGGCA GCAGCCATGT TTGAATCTGG AGACGTTGAT
    GAAGTTACTA ACGGGCTTAC ATCCATGAAC GAACTCATCG TCCCATGTTT GCCCTTGCTG
    CTGGTCGATG ACATGGAGGA GAAGGACATT GTTGCTGTGG AAGACATGAG AAACCGTTGG
    TGTTCCTACC TTGGGCAAGA AATGGAGCCA AATCTGCAAG AAAAGCTGAC CGACTTCCTG
    CCAAAACTGC TTGATTGTTC AACAGAAATC AAAGGTTTAA ACGACCCGCC AAAGCTTCCC
    AACTTCTCCA CGCACGAACT GTGTGAGCGC TTCGCCCGAA TCATGTTGTC ACTTAGCCGA
    ATGCCGGCCG ACGGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28753
    Clone ID Related Accession (Same CDS sequence) XM_012957169.2
    Accession Version XM_012957169.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin carboxyl-terminal hydrolase 25 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCAGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG CCCACTGATA ACGCCCCAGC TCCAGCAGCT
    CCTAACCAAC GAGTTGTAGA GGTGGCGATC CCTCACGTAG GGACTTTTGT TATTGAGTCA
    GAGGAGGGGG GTTATGACGA TGAGGTCATG ATGACCCCGA ACATGCAAGG TATAATCATG
    GCCATAGGTA AATCCCGGAA TGTGTATGAC AAGTGTGGCC CTGAAGCAGG GTTCTTTAAG
    GCCATCAAAA CTGAATACGC TCGGCTAGTG AAACTAGCGC AAGAAGACCC CCCGCCAGAG
    ATAGATTACC GCTTGCAGCA CGCCATAGTC TACTTCATCC AAAGCCAAGC ACCAAAGAAA
    ATCATAGAAA GGACATTGCT GGAGCAGTTT GCAGACCGTA ATTTGAGTTT TGATGAACGA
    TGCCGCAATA TTATGAAAGT TGCTCAGGCT AAACTGGAAA TGATAAAACC AGATGAGGTC
    AACATGGAGG AATACAGGAA GTGGCACAAG GACTATAGAA TGTTCCGAGA GACAACAATG
    TACATCTTGA TTGGTCTGGA GCTGTTTCAG AACAAGAGCT ATGTTGAAGC GCTGCTGTAC
    CTAATTTATG GCTACCAGTA CAATAAAGAG CTGATCTCGA AAGGCCTCTA CAGAGGGCAT
    GATGAGGAGC TCATATCCCA TTACAGAAGG GAGTGCTTTC TGAAACTGAA TGAGCAGGCA
    GCAGCCATGT TTGAATCTGG AGACGTTGAT GAAGTTACTA ACGGGCTTAC ATCCATGAAC
    GAACTCATCG TCCCATGTTT GCCCTTGCTG CTGGTCGATG ACATGGAGGA GAAGGACATT
    GTTGCTGTGG AAGACATGAG AAACCGTTGG TGTTCCTACC TTGGGCAAGA AATGGAGCCA
    AATCTGCAAG AAAAGCTGAC CGACTTCCTG CCAAAACTGC TTGATTGTTC AACAGAAATC
    AAAGGTTTAA ACGACCCGCC AAAGCTTCCC AACTTCTCCA CGCACGAACT GTGTGAGCGC
    TTCGCCCGAA TCATGTTGTC ACTTAGCCGA ATGCCGGCCG ACGGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812623.1
    CDS376..3726
    Translation

    Target ORF information:

    RefSeq Version XM_012957169.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin specific peptidase 25 (usp25), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGG AGCAAAATGT CCTGCAGCAG AACTCCCAGA AGCACCAGCA GACCTTTCTG 
    AATCAGCTGA GGGAGATCAC TGGCATCAAT GACCTCCAAA TACTCCAACA AGCTCTTAAG
    GATAGCAATG GAAATCTGGA GCTAGCTGTT GCCTTTCTCA CTGCAAAGAA TGCCAAGCTT
    CCCCAGCCAG AAGAGGCGAC GTATTATCAG ACTGCACTCC CTAACAATGA CCGATACATC
    AGTGTGGGCA GCCAAGCAGA CACAAATGTT ATTGATCTGA CTGTGGATGA TAAAGATGAT
    CTTCAGAGGG CCATTGCTTT AAGCCTGGAG GAGTCCAACA GGACATTCAG GGAGACCGGA
    ATCACTGATG AGGAGCAAGC CATTAGCAGG GTGTTAGAAG CCAGCATTGC AGAAAACAAA
    GCAAGTCTTA AACGGACACA CACCGAGGTC TGGAGCGACT CTCCCAATCC TTATGATCGC
    AAGCGTCAAG AAAACTGCCC TGTAGGATTA AAGAATGTGG GCAATACGTG CTGGTTCAGC
    GCTGTCATTC AGTCTTTATT TAACCTGCTG GAATTCCGCA GACTGGTTCT CAACTACAGT
    CCCCCTGCAA CTGCTCAGGA TGTACCACGA AACCAAAAGG AACATAGGAA TTTGCCTTTC
    ATGCGGGAGC TGAGATACCT GTTTTCACTA ATGGTCAGCT CCAAAAGGAA GTATGTTGAT
    CCATCCAGAG CTGTTGAGAT TCTTAAGGAC GCTTTCAAAT CAAATGACTC TCAGCAGCAA
    GATGTCAGTG AATTCACTCA CAAACTTTTG GATTGGTTGG AAGATGCTTT CCAGATTAAA
    GCAGAAGAGG AAAGAGATGG AGAAAAGCCA AAGAACCCAA TGGTGGAATT ATTTTATGGA
    AGGTTCCTGG CAGTGGGTGT ACATGAAGGA AAGAAATTTG AGAACACTGA AATGTTTGGC
    CAGTACCCAC TGCAGGTGAA TGGCTTTAAG GACCTGCACG AGTGCCTAGA GGCTGCCATG
    ATTGAAGGGG AAATAGAATC GTTGCACTCA GAAAACTCGG GCAAGTCAGG CCAAGAGCAT
    TGGTTCACGG AGCTCCCGCC TGTGCTAACC TTTGAACTTT CAAGATTCGA ATTCAACCAA
    GCACTGGGAA GGCCTGAGAA AATACACAAC AAACTAGAGT TCCCACCATG TTTGTATATG
    GACAGGTACA TGCACAAGAA CAGGGAAATC ACAAGGCTTA AACGGGATGA AATCAAAAGA
    TTGAAAGAGC ACCTTACAGT GTTACAGCAG AGGCTAGAAC GGTATTTGAG TTATGGTTCT
    GGCCCCAAAC GGTTCCCTCT GGCAGATGTC TTACAATATG CCCTGGAATT TGCATCAAGC
    AAACCTGTTT GCACTTCACC AGTGGAAGAT ATTGATGTAA GTGCGCCACC TAGTGGATGT
    GTTGCAACAC AAACAGTGCA AAGCACAATA GAGCATCAGG GGTCTTCTGC AGCCTCTGAA
    ACGCAGCCTC CTACCCAGCG ATCTGTCATT CACAAACCTT TTACGCAGTC CCGGATACCT
    CCAGATCTAC CTATGCACCC TGCACCCCGA AACATTACAG ACGAGGAACT CTCAGTATTA
    GAGGGGTGTT TGCATCGCTG GAGAACTGAA GTGGAAACTG ATACAAGAGA CCTGCAGGAC
    AGCATAGGAA GAATACACCG GACGATAGAG CTCATGTACT CTGATAAGCA GATGAACCAG
    GTCCCATACA AGCTGCATGC CGTACTTGTC CACGAAGGCC AAGCAAATGC AGGTCACTAC
    TGGGCTTACA TATTTGACCA TCATGAAAAA CGGTGGATGA AATACAATGA CATTTCTGTT
    ACCAAGTCAT CGTGGGAAGA GCTAGAGCGA GATTCCTTCG GGGGGTATAG GAACGCAAGT
    GCTTACTGTC TGATGTATAT CAATGATGAG GAAAAGTATT TAATGCAAGA AGAGTACGAC
    AAGGAGACAG GGCAGGTCCT TACAGGTATG GACACCCTGC CGTTAGATTT AAGGGAGTAT
    GTCAAGGAAG ACAACCGGCA GTTTGAGAAA GAACTTGAAG AGTGGGACAA AGAGCAGCTC
    CGGAAAGCTG AACAGGAGAA GATGATTTTA GCCCAAAAGG TCTTGAAACA GGCTCCATTG
    GTAGTGGCAC CTCCACCAGT CGAGCCGGAA TACATGGAAC AGCCAACAAA AGTAGACACC
    CCTAATGAGA TGAGTGAAGA AACCACTACT GTCATCACTC AAGCAGCCAC TGAATCCGAA
    GATAAAGGGC CCGATGCTGT ATTGCAAATG CCCACTGATA ACGCCCCAGC TCCAGCAGCT
    CCTAACCAAC GAGTTGTAGA GGTGGCGATC CCTCACGTAG GGACTTTTGT TATTGAGTCA
    GAGGAGGGGG GTTATGACGA TGAGGTCATG ATGACCCCGA ACATGCAAGG TATAATCATG
    GCCATAGGTA AATCCCGGAA TGTGTATGAC AAGTGTGGCC CTGAAGCAGG GTTCTTTAAG
    GCCATCAAAA CTGAATACGC TCGGCTAGTG AAACTAGCGC AAGAAGACCC CCCGCCAGAG
    ATAGATTACC GCTTGCAGCA CGCCATAGTC TACTTCATCC AAAGCCAAGC ACCAAAGAAA
    ATCATAGAAA GGACATTGCT GGAGCAGTTT GCAGACCGTA ATTTGAGTTT TGATGAACGA
    TGCCGCAATA TTATGAAAGT TGCTCAGGCT AAACTGGAAA TGATAAAACC AGATGAGGTC
    AACATGGAGG AATACAGGAA GTGGCACAAG GACTATAGAA TGTTCCGAGA GACAACAATG
    TACATCTTGA TTGGTCTGGA GCTGTTTCAG AACAAGAGCT ATGTTGAAGC GCTGCTGTAC
    CTAATTTATG GCTACCAGTA CAATAAAGAG CTGATCTCGA AAGGCCTCTA CAGAGGGCAT
    GATGAGGAGC TCATATCCCA TTACAGAAGG GAGTGCTTTC TGAAACTGAA TGAGCAGGCA
    GCAGCCATGT TTGAATCTGG AGACGTTGAT GAAGTTACTA ACGGGCTTAC ATCCATGAAC
    GAACTCATCG TCCCATGTTT GCCCTTGCTG CTGGTCGATG ACATGGAGGA GAAGGACATT
    GTTGCTGTGG AAGACATGAG AAACCGTTGG TGTTCCTACC TTGGGCAAGA AATGGAGCCA
    AATCTGCAAG AAAAGCTGAC CGACTTCCTG CCAAAACTGC TTGATTGTTC AACAGAAATC
    AAAGGTTTAA ACGACCCGCC AAAGCTTCCC AACTTCTCCA CGCACGAACT GTGTGAGCGC
    TTCGCCCGAA TCATGTTGTC ACTTAGCCGA ATGCCGGCCG ACGGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.