USP25 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005201159.3	XP_005201216.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_015468160.1	XP_015323646.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_010800946.3	XP_010799248.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_010824433.2	XP_010822735.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_010800945.2	XP_010799247.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_010800947.2	XP_010799249.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_015468160.2	XP_015323646.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_005201158.3	XP_005201215.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_010824431.2	XP_010822733.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_015461928.1	XP_015317414.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_602857.8	XP_602857.5	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
XM_005201159.4	XP_005201216.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_010800946.2	XP_010799248.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_002684646.5	XP_002684692.2	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
XM_005195959.3	XP_005196016.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_002684646.6	XP_002684692.2	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
XM_003581669.4	XP_003581717.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_010824432.2	XP_010822734.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_010800945.3	XP_010799247.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_005201158.4	XP_005201215.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_010800947.3	XP_010799249.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa87634	XM_015468160.2 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa87634	XM_015461928.1 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa02437	XM_005201158.4 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02437	XM_003581669.4 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02438	XM_005195959.3 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02438	XM_005201159.4 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02439	XM_602857.8 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02439	XM_002684646.6 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50891	XM_010824431.2 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50891	XM_010800945.3 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50892	XM_010800946.3 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50892	XM_010824432.2 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50893	XM_010800947.3 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50893	XM_010824433.2 Latest version!	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa87634	XM_015468160.1	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa02438	XM_005201159.3	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50892	XM_010800946.2	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02439	XM_002684646.5	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50891	XM_010800945.2	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02437	XM_005201158.3	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa50893	XM_010800947.2	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

USP25 ( XM_015468160.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323646.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa87634
Clone ID Related Accession (Same CDS sequence)	XM_015468160.1 , XM_015468160.2 , XM_015461928.1
Accession Version	XM_015468160.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCGCTT CCTGGCAATG 
ACAGATACAT CAGTGTGGGC AGCCAAGCGG ACACAATTTT GCTTTTCAGA TGTGATTGAT 
CTTACTGGAG ATGATAAAGA TGATCTTCAG AGAGCAATTG CCTTGAGTTT GGCGGAATCA 
AACAGGGCAT TCAGGGAGAC TGGAATAACT GATGAGGAGC AAGCCATTAG CAGAGTTCTT 
GAGGCCAGCA TAGCAGAAAA TAAAGCATGT TTGAAGAGGA CACCTACTGA AGTTTGGAGA 
GATTCTCGAA ACCCTTACGA TAGAAAAAGG CAGGACAAAG CTCCTGTTGG ACTAAAGAAT 
GTTGGCAATA CTTGCTGGTT TAGTGCTGTT ATTCAGTCAT TATTCAATCT TTTGGAATTC 
AGAAGATTAG TTCTGAATTA CAAGCCTCCA TCAAATGTTC AAGATTTACC CCGAAACCAA 
AAGGAGCATC GGAATTTGCC TTTTATGCGT GAGCTTCGAT ATCTATTTGC ACTTCTTGTT 
GGTACTAAAA GGAAATATGT TGATCCATCA AGAGCAGTTG AGATTCTTAA AGATGCTTTC 
AAATCAAATG ACTCCCAGCA GCAAGACGTG AGTGAGTTTA CACACAAATT ATTAGATTGG 
TTAGAAGATG CCTTCCAAAT GAAAGCTGAA GAAGAGACGG ATGAAGACAA GCCAAAGAAC 
CCCATGGTAG AGTTGTTCTA TGGAAGATTC CTAGCTGTGG GAGTGCTTGA AGGTAAAAAA 
TTTGAAAATA CTGAAATGTT TGGTCAGTAC CCACTTCAGG TCAATGGATT TAAAGATCTA 
CATGAATGCC TAGAAGCTGC TATGATTGAA GGAGAAATTG AGTCTTTACA TTCAGAAAAT 
TCAGGAAAAT CTGGTCAAGA GCATTGGTTT ACTGAACTCC CACCTGTGTT AACATTTGAA 
TTGTCAAGAT TTGAATTTAA TCAGGCACTG GGAAGACCAG AAAAAATTCA CAATAAATTA 
GAATTTCCTC AAGTTTTATA TCTGGACAGA TACATGCATA GAAACAGAGA AATAACAAGA 
ATTAAGAGGG AAGAGATCAA GAGACTAAAA GATTACCTCA CAGTATTACA ACAAAGACTT 
GAAAGGTATT TAAGCTATGG TTCGGGTCCC AAACGGTTTC CCTTGGTGGA TGTTCTGCAG 
TACGCCTTAG AATTTGCCTC AAGTAAACCT GTTTGCACTT CTCCTGTTGA TGATATTGAT 
GCTAGTTCCC CATCTAGTGG TTCCGTACCA TCACAGACAT TACCAAGCAC AACAGACCAA 
CAGGGAGCTC CTTCTTCAGA ACTGCCAAGC ACGTCACCCG CGTCAATAGC TGCCATTTCA 
TCAAGATCAG TAATACACAA ACCATTCACT CAGTCTCGGA TACCTCCAGA CTTGCCCATG 
CATCCGGCAC CAAGGCACAT AACAGAAGAA GAACTCTCTG TGCTAGAAAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTAC AGGAAAGCAT ATCTAGAATC 
CATCGCACAA TTGAGCTAAT GTACTCTGAC AAATCTATGA TCCAAGTTCC TTATCGTTTA 
CATGCTGTTT TAGTTCATGA AGGTCAAGCT AATGCCGGGC ACTACTGGGC ATACATTTTT 
GATCATCGTG AAAACAGGTG GATGAAGTAC AATGATATTG CTGTAACAAA ATCATCATGG 
GAAGAGCTAG TGAGGGATTC TTTTGGTGGT TACAGAAATG CCAGTGCATA CTGTTTAATG 
TACATAAATG ACAAACCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGGCAG 
GCCCTTGTTG GAATAGAAAC ACTACCCCCT GACTTAAGAG ATTTTGTTGA GGAAGACAAC 
CAGCGATTTG AGAAAGAACT AGAAGAATGG GATGCACAAC TTGCTCAGAA AGCCTTGCAG 
GAAAAACTTC TAGCATCTCA GAAATTGAGA GAGTCCGAGT CTTCTGTGAC AACAGCACAA 
GCAGGAGAAC CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA 
GAAGAAACCA TTCGAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTGC AGTCGAAACC TGAAAATACC ACAAGCCAGC CACCTTCTAA CCAGCAAGTT 
GTAGAGGTGG AGATTCCTCA TGTAGGGACA TTTATGGTTG AGTCAAAGGA GGGGGGTTAT 
GATGATGAGA TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCCAT AGGTAAATCC 
CGGAGTGTGT ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA 
TATTCAAGGT TGGTTAAATT GGCCCAAGAA GATACCCCAC CAGAAACAGA CTATCGTTTA 
CATCATGTAA TAGTCTACTT TATCCAGAAT CAGGCTCCAA AGAAAATCAT TGAGAAAACA 
TTGCTAGAAC AATTTGCAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAAAT TGAAATGATA AAACCTGAAG AAGTAAACAT GGAGGAGTAT 
GAGGAGTGGC ATCAGGATTA TAGAAAATTC AGGGAAACAA CCATGTATCT CATTATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TCTTCCTTAT CTGTGCTTAT 
CAGAATAACA AAGAACTGTT GTCCAGAGGC CCATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTACTAAAA TTAAATGAGC AAGCTGCAGA ACTGTTTGAA 
TCTGGAGAGG ATAGAGAAGT AAACAATGGT CTGATTATCA TGAATGAATT TATTGTCCCA 
TTTTTGCCCT TGTTATTGGT GGATGAAATG GAAGAGAAAG ACATACTTGC TGTGGAAGAT 
ATGAGAAATC GATGGTGTTC CTATCTTGGA CAAGAAATGG AATCAAACCT CCAAGAGAAG 
CTGACAGATT TTCTGCCAAA GCTGCTTGAT TGTTCTACGG AGATTAAAGG TTTCCATGAG 
CCGCCTAAGT TACCTCCGTA CTCCACACAC GAACTCTGTG AGCGATTTAC CCGAATCATG 
CTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323646.1
CDS	128..3343
Translation	MPRPLSRRRQLTTKQRFLAMTDTSVWAAKRTQFCFSDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_015468160.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468160.1

ORF Insert Sequence:

ATGCCAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCGCTT CCTGGCAATG 
ACAGATACAT CAGTGTGGGC AGCCAAGCGG ACACAATTTT GCTTTTCAGA TGTGATTGAT 
CTTACTGGAG ATGATAAAGA TGATCTTCAG AGAGCAATTG CCTTGAGTTT GGCGGAATCA 
AACAGGGCAT TCAGGGAGAC TGGAATAACT GATGAGGAGC AAGCCATTAG CAGAGTTCTT 
GAGGCCAGCA TAGCAGAAAA TAAAGCATGT TTGAAGAGGA CACCTACTGA AGTTTGGAGA 
GATTCTCGAA ACCCTTACGA TAGAAAAAGG CAGGACAAAG CTCCTGTTGG ACTAAAGAAT 
GTTGGCAATA CTTGCTGGTT TAGTGCTGTT ATTCAGTCAT TATTCAATCT TTTGGAATTC 
AGAAGATTAG TTCTGAATTA CAAGCCTCCA TCAAATGTTC AAGATTTACC CCGAAACCAA 
AAGGAGCATC GGAATTTGCC TTTTATGCGT GAGCTTCGAT ATCTATTTGC ACTTCTTGTT 
GGTACTAAAA GGAAATATGT TGATCCATCA AGAGCAGTTG AGATTCTTAA AGATGCTTTC 
AAATCAAATG ACTCCCAGCA GCAAGACGTG AGTGAGTTTA CACACAAATT ATTAGATTGG 
TTAGAAGATG CCTTCCAAAT GAAAGCTGAA GAAGAGACGG ATGAAGACAA GCCAAAGAAC 
CCCATGGTAG AGTTGTTCTA TGGAAGATTC CTAGCTGTGG GAGTGCTTGA AGGTAAAAAA 
TTTGAAAATA CTGAAATGTT TGGTCAGTAC CCACTTCAGG TCAATGGATT TAAAGATCTA 
CATGAATGCC TAGAAGCTGC TATGATTGAA GGAGAAATTG AGTCTTTACA TTCAGAAAAT 
TCAGGAAAAT CTGGTCAAGA GCATTGGTTT ACTGAACTCC CACCTGTGTT AACATTTGAA 
TTGTCAAGAT TTGAATTTAA TCAGGCACTG GGAAGACCAG AAAAAATTCA CAATAAATTA 
GAATTTCCTC AAGTTTTATA TCTGGACAGA TACATGCATA GAAACAGAGA AATAACAAGA 
ATTAAGAGGG AAGAGATCAA GAGACTAAAA GATTACCTCA CAGTATTACA ACAAAGACTT 
GAAAGGTATT TAAGCTATGG TTCGGGTCCC AAACGGTTTC CCTTGGTGGA TGTTCTGCAG 
TACGCCTTAG AATTTGCCTC AAGTAAACCT GTTTGCACTT CTCCTGTTGA TGATATTGAT 
GCTAGTTCCC CATCTAGTGG TTCCGTACCA TCACAGACAT TACCAAGCAC AACAGACCAA 
CAGGGAGCTC CTTCTTCAGA ACTGCCAAGC ACGTCACCCG CGTCAATAGC TGCCATTTCA 
TCAAGATCAG TAATACACAA ACCATTCACT CAGTCTCGGA TACCTCCAGA CTTGCCCATG 
CATCCGGCAC CAAGGCACAT AACAGAAGAA GAACTCTCTG TGCTAGAAAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTAC AGGAAAGCAT ATCTAGAATC 
CATCGCACAA TTGAGCTAAT GTACTCTGAC AAATCTATGA TCCAAGTTCC TTATCGTTTA 
CATGCTGTTT TAGTTCATGA AGGTCAAGCT AATGCCGGGC ACTACTGGGC ATACATTTTT 
GATCATCGTG AAAACAGGTG GATGAAGTAC AATGATATTG CTGTAACAAA ATCATCATGG 
GAAGAGCTAG TGAGGGATTC TTTTGGTGGT TACAGAAATG CCAGTGCATA CTGTTTAATG 
TACATAAATG ACAAACCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGGCAG 
GCCCTTGTTG GAATAGAAAC ACTACCCCCT GACTTAAGAG ATTTTGTTGA GGAAGACAAC 
CAGCGATTTG AGAAAGAACT AGAAGAATGG GATGCACAAC TTGCTCAGAA AGCCTTGCAG 
GAAAAACTTC TAGCATCTCA GAAATTGAGA GAGTCCGAGT CTTCTGTGAC AACAGCACAA 
GCAGGAGAAC CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA 
GAAGAAACCA TTCGAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTGC AGTCGAAACC TGAAAATACC ACAAGCCAGC CACCTTCTAA CCAGCAAGTT 
GTAGAGGTGG AGATTCCTCA TGTAGGGACA TTTATGGTTG AGTCAAAGGA GGGGGGTTAT 
GATGATGAGA TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCCAT AGGTAAATCC 
CGGAGTGTGT ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA 
TATTCAAGGT TGGTTAAATT GGCCCAAGAA GATACCCCAC CAGAAACAGA CTATCGTTTA 
CATCATGTAA TAGTCTACTT TATCCAGAAT CAGGCTCCAA AGAAAATCAT TGAGAAAACA 
TTGCTAGAAC AATTTGCAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAAAT TGAAATGATA AAACCTGAAG AAGTAAACAT GGAGGAGTAT 
GAGGAGTGGC ATCAGGATTA TAGAAAATTC AGGGAAACAA CCATGTATCT CATTATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TCTTCCTTAT CTGTGCTTAT 
CAGAATAACA AAGAACTGTT GTCCAGAGGC CCATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTACTAAAA TTAAATGAGC AAGCTGCAGA ACTGTTTGAA 
TCTGGAGAGG ATAGAGAAGT AAACAATGGT CTGATTATCA TGAATGAATT TATTGTCCCA 
TTTTTGCCCT TGTTATTGGT GGATGAAATG GAAGAGAAAG ACATACTTGC TGTGGAAGAT 
ATGAGAAATC GATGGTGTTC CTATCTTGGA CAAGAAATGG AATCAAACCT CCAAGAGAAG 
CTGACAGATT TTCTGCCAAA GCTGCTTGAT TGTTCTACGG AGATTAAAGG TTTCCATGAG 
CCGCCTAAGT TACCTCCGTA CTCCACACAC GAACTCTGTG AGCGATTTAC CCGAATCATG 
CTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_015468160.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323646.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa87634
Clone ID Related Accession (Same CDS sequence)	XM_015468160.1 , XM_015468160.2 , XM_015461928.1
Accession Version	XM_015468160.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015468160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCGCTT CCTGGCAATG 
ACAGATACAT CAGTGTGGGC AGCCAAGCGG ACACAATTTT GCTTTTCAGA TGTGATTGAT 
CTTACTGGAG ATGATAAAGA TGATCTTCAG AGAGCAATTG CCTTGAGTTT GGCGGAATCA 
AACAGGGCAT TCAGGGAGAC TGGAATAACT GATGAGGAGC AAGCCATTAG CAGAGTTCTT 
GAGGCCAGCA TAGCAGAAAA TAAAGCATGT TTGAAGAGGA CACCTACTGA AGTTTGGAGA 
GATTCTCGAA ACCCTTACGA TAGAAAAAGG CAGGACAAAG CTCCTGTTGG ACTAAAGAAT 
GTTGGCAATA CTTGCTGGTT TAGTGCTGTT ATTCAGTCAT TATTCAATCT TTTGGAATTC 
AGAAGATTAG TTCTGAATTA CAAGCCTCCA TCAAATGTTC AAGATTTACC CCGAAACCAA 
AAGGAGCATC GGAATTTGCC TTTTATGCGT GAGCTTCGAT ATCTATTTGC ACTTCTTGTT 
GGTACTAAAA GGAAATATGT TGATCCATCA AGAGCAGTTG AGATTCTTAA AGATGCTTTC 
AAATCAAATG ACTCCCAGCA GCAAGACGTG AGTGAGTTTA CACACAAATT ATTAGATTGG 
TTAGAAGATG CCTTCCAAAT GAAAGCTGAA GAAGAGACGG ATGAAGACAA GCCAAAGAAC 
CCCATGGTAG AGTTGTTCTA TGGAAGATTC CTAGCTGTGG GAGTGCTTGA AGGTAAAAAA 
TTTGAAAATA CTGAAATGTT TGGTCAGTAC CCACTTCAGG TCAATGGATT TAAAGATCTA 
CATGAATGCC TAGAAGCTGC TATGATTGAA GGAGAAATTG AGTCTTTACA TTCAGAAAAT 
TCAGGAAAAT CTGGTCAAGA GCATTGGTTT ACTGAACTCC CACCTGTGTT AACATTTGAA 
TTGTCAAGAT TTGAATTTAA TCAGGCACTG GGAAGACCAG AAAAAATTCA CAATAAATTA 
GAATTTCCTC AAGTTTTATA TCTGGACAGA TACATGCATA GAAACAGAGA AATAACAAGA 
ATTAAGAGGG AAGAGATCAA GAGACTAAAA GATTACCTCA CAGTATTACA ACAAAGACTT 
GAAAGGTATT TAAGCTATGG TTCGGGTCCC AAACGGTTTC CCTTGGTGGA TGTTCTGCAG 
TACGCCTTAG AATTTGCCTC AAGTAAACCT GTTTGCACTT CTCCTGTTGA TGATATTGAT 
GCTAGTTCCC CATCTAGTGG TTCCGTACCA TCACAGACAT TACCAAGCAC AACAGACCAA 
CAGGGAGCTC CTTCTTCAGA ACTGCCAAGC ACGTCACCCG CGTCAATAGC TGCCATTTCA 
TCAAGATCAG TAATACACAA ACCATTCACT CAGTCTCGGA TACCTCCAGA CTTGCCCATG 
CATCCGGCAC CAAGGCACAT AACAGAAGAA GAACTCTCTG TGCTAGAAAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTAC AGGAAAGCAT ATCTAGAATC 
CATCGCACAA TTGAGCTAAT GTACTCTGAC AAATCTATGA TCCAAGTTCC TTATCGTTTA 
CATGCTGTTT TAGTTCATGA AGGTCAAGCT AATGCCGGGC ACTACTGGGC ATACATTTTT 
GATCATCGTG AAAACAGGTG GATGAAGTAC AATGATATTG CTGTAACAAA ATCATCATGG 
GAAGAGCTAG TGAGGGATTC TTTTGGTGGT TACAGAAATG CCAGTGCATA CTGTTTAATG 
TACATAAATG ACAAACCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGGCAG 
GCCCTTGTTG GAATAGAAAC ACTACCCCCT GACTTAAGAG ATTTTGTTGA GGAAGACAAC 
CAGCGATTTG AGAAAGAACT AGAAGAATGG GATGCACAAC TTGCTCAGAA AGCCTTGCAG 
GAAAAACTTC TAGCATCTCA GAAATTGAGA GAGTCCGAGT CTTCTGTGAC AACAGCACAA 
GCAGGAGAAC CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA 
GAAGAAACCA TTCGAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTGC AGTCGAAACC TGAAAATACC ACAAGCCAGC CACCTTCTAA CCAGCAAGTT 
GTAGAGGTGG AGATTCCTCA TGTAGGGACA TTTATGGTTG AGTCAAAGGA GGGGGGTTAT 
GATGATGAGA TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCCAT AGGTAAATCC 
CGGAGTGTGT ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA 
TATTCAAGGT TGGTTAAATT GGCCCAAGAA GATACCCCAC CAGAAACAGA CTATCGTTTA 
CATCATGTAA TAGTCTACTT TATCCAGAAT CAGGCTCCAA AGAAAATCAT TGAGAAAACA 
TTGCTAGAAC AATTTGCAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAAAT TGAAATGATA AAACCTGAAG AAGTAAACAT GGAGGAGTAT 
GAGGAGTGGC ATCAGGATTA TAGAAAATTC AGGGAAACAA CCATGTATCT CATTATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TCTTCCTTAT CTGTGCTTAT 
CAGAATAACA AAGAACTGTT GTCCAGAGGC CCATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTACTAAAA TTAAATGAGC AAGCTGCAGA ACTGTTTGAA 
TCTGGAGAGG ATAGAGAAGT AAACAATGGT CTGATTATCA TGAATGAATT TATTGTCCCA 
TTTTTGCCCT TGTTATTGGT GGATGAAATG GAAGAGAAAG ACATACTTGC TGTGGAAGAT 
ATGAGAAATC GATGGTGTTC CTATCTTGGA CAAGAAATGG AATCAAACCT CCAAGAGAAG 
CTGACAGATT TTCTGCCAAA GCTGCTTGAT TGTTCTACGG AGATTAAAGG TTTCCATGAG 
CCGCCTAAGT TACCTCCGTA CTCCACACAC GAACTCTGTG AGCGATTTAC CCGAATCATG 
CTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323646.1
CDS	133..3348
Translation	MPRPLSRRRQLTTKQRFLAMTDTSVWAAKRTQFCFSDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_015468160.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468160.2

ORF Insert Sequence:

ATGCCAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCGCTT CCTGGCAATG 
ACAGATACAT CAGTGTGGGC AGCCAAGCGG ACACAATTTT GCTTTTCAGA TGTGATTGAT 
CTTACTGGAG ATGATAAAGA TGATCTTCAG AGAGCAATTG CCTTGAGTTT GGCGGAATCA 
AACAGGGCAT TCAGGGAGAC TGGAATAACT GATGAGGAGC AAGCCATTAG CAGAGTTCTT 
GAGGCCAGCA TAGCAGAAAA TAAAGCATGT TTGAAGAGGA CACCTACTGA AGTTTGGAGA 
GATTCTCGAA ACCCTTACGA TAGAAAAAGG CAGGACAAAG CTCCTGTTGG ACTAAAGAAT 
GTTGGCAATA CTTGCTGGTT TAGTGCTGTT ATTCAGTCAT TATTCAATCT TTTGGAATTC 
AGAAGATTAG TTCTGAATTA CAAGCCTCCA TCAAATGTTC AAGATTTACC CCGAAACCAA 
AAGGAGCATC GGAATTTGCC TTTTATGCGT GAGCTTCGAT ATCTATTTGC ACTTCTTGTT 
GGTACTAAAA GGAAATATGT TGATCCATCA AGAGCAGTTG AGATTCTTAA AGATGCTTTC 
AAATCAAATG ACTCCCAGCA GCAAGACGTG AGTGAGTTTA CACACAAATT ATTAGATTGG 
TTAGAAGATG CCTTCCAAAT GAAAGCTGAA GAAGAGACGG ATGAAGACAA GCCAAAGAAC 
CCCATGGTAG AGTTGTTCTA TGGAAGATTC CTAGCTGTGG GAGTGCTTGA AGGTAAAAAA 
TTTGAAAATA CTGAAATGTT TGGTCAGTAC CCACTTCAGG TCAATGGATT TAAAGATCTA 
CATGAATGCC TAGAAGCTGC TATGATTGAA GGAGAAATTG AGTCTTTACA TTCAGAAAAT 
TCAGGAAAAT CTGGTCAAGA GCATTGGTTT ACTGAACTCC CACCTGTGTT AACATTTGAA 
TTGTCAAGAT TTGAATTTAA TCAGGCACTG GGAAGACCAG AAAAAATTCA CAATAAATTA 
GAATTTCCTC AAGTTTTATA TCTGGACAGA TACATGCATA GAAACAGAGA AATAACAAGA 
ATTAAGAGGG AAGAGATCAA GAGACTAAAA GATTACCTCA CAGTATTACA ACAAAGACTT 
GAAAGGTATT TAAGCTATGG TTCGGGTCCC AAACGGTTTC CCTTGGTGGA TGTTCTGCAG 
TACGCCTTAG AATTTGCCTC AAGTAAACCT GTTTGCACTT CTCCTGTTGA TGATATTGAT 
GCTAGTTCCC CATCTAGTGG TTCCGTACCA TCACAGACAT TACCAAGCAC AACAGACCAA 
CAGGGAGCTC CTTCTTCAGA ACTGCCAAGC ACGTCACCCG CGTCAATAGC TGCCATTTCA 
TCAAGATCAG TAATACACAA ACCATTCACT CAGTCTCGGA TACCTCCAGA CTTGCCCATG 
CATCCGGCAC CAAGGCACAT AACAGAAGAA GAACTCTCTG TGCTAGAAAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTAC AGGAAAGCAT ATCTAGAATC 
CATCGCACAA TTGAGCTAAT GTACTCTGAC AAATCTATGA TCCAAGTTCC TTATCGTTTA 
CATGCTGTTT TAGTTCATGA AGGTCAAGCT AATGCCGGGC ACTACTGGGC ATACATTTTT 
GATCATCGTG AAAACAGGTG GATGAAGTAC AATGATATTG CTGTAACAAA ATCATCATGG 
GAAGAGCTAG TGAGGGATTC TTTTGGTGGT TACAGAAATG CCAGTGCATA CTGTTTAATG 
TACATAAATG ACAAACCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGGCAG 
GCCCTTGTTG GAATAGAAAC ACTACCCCCT GACTTAAGAG ATTTTGTTGA GGAAGACAAC 
CAGCGATTTG AGAAAGAACT AGAAGAATGG GATGCACAAC TTGCTCAGAA AGCCTTGCAG 
GAAAAACTTC TAGCATCTCA GAAATTGAGA GAGTCCGAGT CTTCTGTGAC AACAGCACAA 
GCAGGAGAAC CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA 
GAAGAAACCA TTCGAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTGC AGTCGAAACC TGAAAATACC ACAAGCCAGC CACCTTCTAA CCAGCAAGTT 
GTAGAGGTGG AGATTCCTCA TGTAGGGACA TTTATGGTTG AGTCAAAGGA GGGGGGTTAT 
GATGATGAGA TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCCAT AGGTAAATCC 
CGGAGTGTGT ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA 
TATTCAAGGT TGGTTAAATT GGCCCAAGAA GATACCCCAC CAGAAACAGA CTATCGTTTA 
CATCATGTAA TAGTCTACTT TATCCAGAAT CAGGCTCCAA AGAAAATCAT TGAGAAAACA 
TTGCTAGAAC AATTTGCAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAAAT TGAAATGATA AAACCTGAAG AAGTAAACAT GGAGGAGTAT 
GAGGAGTGGC ATCAGGATTA TAGAAAATTC AGGGAAACAA CCATGTATCT CATTATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TCTTCCTTAT CTGTGCTTAT 
CAGAATAACA AAGAACTGTT GTCCAGAGGC CCATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTACTAAAA TTAAATGAGC AAGCTGCAGA ACTGTTTGAA 
TCTGGAGAGG ATAGAGAAGT AAACAATGGT CTGATTATCA TGAATGAATT TATTGTCCCA 
TTTTTGCCCT TGTTATTGGT GGATGAAATG GAAGAGAAAG ACATACTTGC TGTGGAAGAT 
ATGAGAAATC GATGGTGTTC CTATCTTGGA CAAGAAATGG AATCAAACCT CCAAGAGAAG 
CTGACAGATT TTCTGCCAAA GCTGCTTGAT TGTTCTACGG AGATTAAAGG TTTCCATGAG 
CCGCCTAAGT TACCTCCGTA CTCCACACAC GAACTCTGTG AGCGATTTAC CCGAATCATG 
CTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_015461928.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317414.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa87634
Clone ID Related Accession (Same CDS sequence)	XM_015468160.1 , XM_015468160.2 , XM_015461928.1
Accession Version	XM_015461928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCGCTT CCTGGCAATG 
ACAGATACAT CAGTGTGGGC AGCCAAGCGG ACACAATTTT GCTTTTCAGA TGTGATTGAT 
CTTACTGGAG ATGATAAAGA TGATCTTCAG AGAGCAATTG CCTTGAGTTT GGCGGAATCA 
AACAGGGCAT TCAGGGAGAC TGGAATAACT GATGAGGAGC AAGCCATTAG CAGAGTTCTT 
GAGGCCAGCA TAGCAGAAAA TAAAGCATGT TTGAAGAGGA CACCTACTGA AGTTTGGAGA 
GATTCTCGAA ACCCTTACGA TAGAAAAAGG CAGGACAAAG CTCCTGTTGG ACTAAAGAAT 
GTTGGCAATA CTTGCTGGTT TAGTGCTGTT ATTCAGTCAT TATTCAATCT TTTGGAATTC 
AGAAGATTAG TTCTGAATTA CAAGCCTCCA TCAAATGTTC AAGATTTACC CCGAAACCAA 
AAGGAGCATC GGAATTTGCC TTTTATGCGT GAGCTTCGAT ATCTATTTGC ACTTCTTGTT 
GGTACTAAAA GGAAATATGT TGATCCATCA AGAGCAGTTG AGATTCTTAA AGATGCTTTC 
AAATCAAATG ACTCCCAGCA GCAAGACGTG AGTGAGTTTA CACACAAATT ATTAGATTGG 
TTAGAAGATG CCTTCCAAAT GAAAGCTGAA GAAGAGACGG ATGAAGACAA GCCAAAGAAC 
CCCATGGTAG AGTTGTTCTA TGGAAGATTC CTAGCTGTGG GAGTGCTTGA AGGTAAAAAA 
TTTGAAAATA CTGAAATGTT TGGTCAGTAC CCACTTCAGG TCAATGGATT TAAAGATCTA 
CATGAATGCC TAGAAGCTGC TATGATTGAA GGAGAAATTG AGTCTTTACA TTCAGAAAAT 
TCAGGAAAAT CTGGTCAAGA GCATTGGTTT ACTGAACTCC CACCTGTGTT AACATTTGAA 
TTGTCAAGAT TTGAATTTAA TCAGGCACTG GGAAGACCAG AAAAAATTCA CAATAAATTA 
GAATTTCCTC AAGTTTTATA TCTGGACAGA TACATGCATA GAAACAGAGA AATAACAAGA 
ATTAAGAGGG AAGAGATCAA GAGACTAAAA GATTACCTCA CAGTATTACA ACAAAGACTT 
GAAAGGTATT TAAGCTATGG TTCGGGTCCC AAACGGTTTC CCTTGGTGGA TGTTCTGCAG 
TACGCCTTAG AATTTGCCTC AAGTAAACCT GTTTGCACTT CTCCTGTTGA TGATATTGAT 
GCTAGTTCCC CATCTAGTGG TTCCGTACCA TCACAGACAT TACCAAGCAC AACAGACCAA 
CAGGGAGCTC CTTCTTCAGA ACTGCCAAGC ACGTCACCCG CGTCAATAGC TGCCATTTCA 
TCAAGATCAG TAATACACAA ACCATTCACT CAGTCTCGGA TACCTCCAGA CTTGCCCATG 
CATCCGGCAC CAAGGCACAT AACAGAAGAA GAACTCTCTG TGCTAGAAAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTAC AGGAAAGCAT ATCTAGAATC 
CATCGCACAA TTGAGCTAAT GTACTCTGAC AAATCTATGA TCCAAGTTCC TTATCGTTTA 
CATGCTGTTT TAGTTCATGA AGGTCAAGCT AATGCCGGGC ACTACTGGGC ATACATTTTT 
GATCATCGTG AAAACAGGTG GATGAAGTAC AATGATATTG CTGTAACAAA ATCATCATGG 
GAAGAGCTAG TGAGGGATTC TTTTGGTGGT TACAGAAATG CCAGTGCATA CTGTTTAATG 
TACATAAATG ACAAACCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGGCAG 
GCCCTTGTTG GAATAGAAAC ACTACCCCCT GACTTAAGAG ATTTTGTTGA GGAAGACAAC 
CAGCGATTTG AGAAAGAACT AGAAGAATGG GATGCACAAC TTGCTCAGAA AGCCTTGCAG 
GAAAAACTTC TAGCATCTCA GAAATTGAGA GAGTCCGAGT CTTCTGTGAC AACAGCACAA 
GCAGGAGAAC CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA 
GAAGAAACCA TTCGAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTGC AGTCGAAACC TGAAAATACC ACAAGCCAGC CACCTTCTAA CCAGCAAGTT 
GTAGAGGTGG AGATTCCTCA TGTAGGGACA TTTATGGTTG AGTCAAAGGA GGGGGGTTAT 
GATGATGAGA TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCCAT AGGTAAATCC 
CGGAGTGTGT ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA 
TATTCAAGGT TGGTTAAATT GGCCCAAGAA GATACCCCAC CAGAAACAGA CTATCGTTTA 
CATCATGTAA TAGTCTACTT TATCCAGAAT CAGGCTCCAA AGAAAATCAT TGAGAAAACA 
TTGCTAGAAC AATTTGCAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAAAT TGAAATGATA AAACCTGAAG AAGTAAACAT GGAGGAGTAT 
GAGGAGTGGC ATCAGGATTA TAGAAAATTC AGGGAAACAA CCATGTATCT CATTATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TCTTCCTTAT CTGTGCTTAT 
CAGAATAACA AAGAACTGTT GTCCAGAGGC CCATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTACTAAAA TTAAATGAGC AAGCTGCAGA ACTGTTTGAA 
TCTGGAGAGG ATAGAGAAGT AAACAATGGT CTGATTATCA TGAATGAATT TATTGTCCCA 
TTTTTGCCCT TGTTATTGGT GGATGAAATG GAAGAGAAAG ACATACTTGC TGTGGAAGAT 
ATGAGAAATC GATGGTGTTC CTATCTTGGA CAAGAAATGG AATCAAACCT CCAAGAGAAG 
CTGACAGATT TTCTGCCAAA GCTGCTTGAT TGTTCTACGG AGATTAAAGG TTTCCATGAG 
CCGCCTAAGT TACCTCCGTA CTCCACACAC GAACTCTGTG AGCGATTTAC CCGAATCATG 
CTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317414.1
CDS	128..3343
Translation	MPRPLSRRRQLTTKQRFLAMTDTSVWAAKRTQFCFSDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_015461928.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461928.1

ORF Insert Sequence:

ATGCCAAGAC CCCTCAGCAG GAGGAGACAA CTTACTACCA AACAGCGCTT CCTGGCAATG 
ACAGATACAT CAGTGTGGGC AGCCAAGCGG ACACAATTTT GCTTTTCAGA TGTGATTGAT 
CTTACTGGAG ATGATAAAGA TGATCTTCAG AGAGCAATTG CCTTGAGTTT GGCGGAATCA 
AACAGGGCAT TCAGGGAGAC TGGAATAACT GATGAGGAGC AAGCCATTAG CAGAGTTCTT 
GAGGCCAGCA TAGCAGAAAA TAAAGCATGT TTGAAGAGGA CACCTACTGA AGTTTGGAGA 
GATTCTCGAA ACCCTTACGA TAGAAAAAGG CAGGACAAAG CTCCTGTTGG ACTAAAGAAT 
GTTGGCAATA CTTGCTGGTT TAGTGCTGTT ATTCAGTCAT TATTCAATCT TTTGGAATTC 
AGAAGATTAG TTCTGAATTA CAAGCCTCCA TCAAATGTTC AAGATTTACC CCGAAACCAA 
AAGGAGCATC GGAATTTGCC TTTTATGCGT GAGCTTCGAT ATCTATTTGC ACTTCTTGTT 
GGTACTAAAA GGAAATATGT TGATCCATCA AGAGCAGTTG AGATTCTTAA AGATGCTTTC 
AAATCAAATG ACTCCCAGCA GCAAGACGTG AGTGAGTTTA CACACAAATT ATTAGATTGG 
TTAGAAGATG CCTTCCAAAT GAAAGCTGAA GAAGAGACGG ATGAAGACAA GCCAAAGAAC 
CCCATGGTAG AGTTGTTCTA TGGAAGATTC CTAGCTGTGG GAGTGCTTGA AGGTAAAAAA 
TTTGAAAATA CTGAAATGTT TGGTCAGTAC CCACTTCAGG TCAATGGATT TAAAGATCTA 
CATGAATGCC TAGAAGCTGC TATGATTGAA GGAGAAATTG AGTCTTTACA TTCAGAAAAT 
TCAGGAAAAT CTGGTCAAGA GCATTGGTTT ACTGAACTCC CACCTGTGTT AACATTTGAA 
TTGTCAAGAT TTGAATTTAA TCAGGCACTG GGAAGACCAG AAAAAATTCA CAATAAATTA 
GAATTTCCTC AAGTTTTATA TCTGGACAGA TACATGCATA GAAACAGAGA AATAACAAGA 
ATTAAGAGGG AAGAGATCAA GAGACTAAAA GATTACCTCA CAGTATTACA ACAAAGACTT 
GAAAGGTATT TAAGCTATGG TTCGGGTCCC AAACGGTTTC CCTTGGTGGA TGTTCTGCAG 
TACGCCTTAG AATTTGCCTC AAGTAAACCT GTTTGCACTT CTCCTGTTGA TGATATTGAT 
GCTAGTTCCC CATCTAGTGG TTCCGTACCA TCACAGACAT TACCAAGCAC AACAGACCAA 
CAGGGAGCTC CTTCTTCAGA ACTGCCAAGC ACGTCACCCG CGTCAATAGC TGCCATTTCA 
TCAAGATCAG TAATACACAA ACCATTCACT CAGTCTCGGA TACCTCCAGA CTTGCCCATG 
CATCCGGCAC CAAGGCACAT AACAGAAGAA GAACTCTCTG TGCTAGAAAG CTGTTTACAT 
CGCTGGAGGA CAGAAATAGA AAATGACACC AGAGATTTAC AGGAAAGCAT ATCTAGAATC 
CATCGCACAA TTGAGCTAAT GTACTCTGAC AAATCTATGA TCCAAGTTCC TTATCGTTTA 
CATGCTGTTT TAGTTCATGA AGGTCAAGCT AATGCCGGGC ACTACTGGGC ATACATTTTT 
GATCATCGTG AAAACAGGTG GATGAAGTAC AATGATATTG CTGTAACAAA ATCATCATGG 
GAAGAGCTAG TGAGGGATTC TTTTGGTGGT TACAGAAATG CCAGTGCATA CTGTTTAATG 
TACATAAATG ACAAACCACA ATTCTTAATA CAAGAGGAGT TTAATAAAGA AACTGGGCAG 
GCCCTTGTTG GAATAGAAAC ACTACCCCCT GACTTAAGAG ATTTTGTTGA GGAAGACAAC 
CAGCGATTTG AGAAAGAACT AGAAGAATGG GATGCACAAC TTGCTCAGAA AGCCTTGCAG 
GAAAAACTTC TAGCATCTCA GAAATTGAGA GAGTCCGAGT CTTCTGTGAC AACAGCACAA 
GCAGGAGAAC CAGAATATCT AGAGCAGCCA TCAAGAAGTG ATTTCTCAAA GCACTTGAAA 
GAAGAAACCA TTCGAATAAT TACCAAGGCA TCACATGAGC ATGAAGATAA AAGTCCTGAA 
ACAGTTTTGC AGTCGAAACC TGAAAATACC ACAAGCCAGC CACCTTCTAA CCAGCAAGTT 
GTAGAGGTGG AGATTCCTCA TGTAGGGACA TTTATGGTTG AGTCAAAGGA GGGGGGTTAT 
GATGATGAGA TCATGATGAC ACCGAACATG CAAGGTATTA TCATGGCCAT AGGTAAATCC 
CGGAGTGTGT ATGACAGGTG TGGCCCTGAA GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA 
TATTCAAGGT TGGTTAAATT GGCCCAAGAA GATACCCCAC CAGAAACAGA CTATCGTTTA 
CATCATGTAA TAGTCTACTT TATCCAGAAT CAGGCTCCAA AGAAAATCAT TGAGAAAACA 
TTGCTAGAAC AATTTGCAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAAAT TGAAATGATA AAACCTGAAG AAGTAAACAT GGAGGAGTAT 
GAGGAGTGGC ATCAGGATTA TAGAAAATTC AGGGAAACAA CCATGTATCT CATTATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TCTTCCTTAT CTGTGCTTAT 
CAGAATAACA AAGAACTGTT GTCCAGAGGC CCATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTACTAAAA TTAAATGAGC AAGCTGCAGA ACTGTTTGAA 
TCTGGAGAGG ATAGAGAAGT AAACAATGGT CTGATTATCA TGAATGAATT TATTGTCCCA 
TTTTTGCCCT TGTTATTGGT GGATGAAATG GAAGAGAAAG ACATACTTGC TGTGGAAGAT 
ATGAGAAATC GATGGTGTTC CTATCTTGGA CAAGAAATGG AATCAAACCT CCAAGAGAAG 
CTGACAGATT TTCTGCCAAA GCTGCTTGAT TGTTCTACGG AGATTAAAGG TTTCCATGAG 
CCGCCTAAGT TACCTCCGTA CTCCACACAC GAACTCTGTG AGCGATTTAC CCGAATCATG 
CTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_005201158.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201215.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa02437
Clone ID Related Accession (Same CDS sequence)	XM_005201158.3 , XM_003581669.4 , XM_005201158.4
Accession Version	XM_005201158.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201158.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACCA CAAGCCAGCC ACCTTCTAAC CAGCAAGTTG TAGAGGTGGA GATTCCTCAT 
GTAGGGACAT TTATGGTTGA GTCAAAGGAG GGGGGTTATG ATGATGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCCATA GGTAAATCCC GGAGTGTGTA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATTCAAGGTT GGTTAAATTG 
GCCCAAGAAG ATACCCCACC AGAAACAGAC TATCGTTTAC ATCATGTAAT AGTCTACTTT 
ATCCAGAATC AGGCTCCAAA GAAAATCATT GAGAAAACAT TGCTAGAACA ATTTGCAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAAATT 
GAAATGATAA AACCTGAAGA AGTAAACATG GAGGAGTATG AGGAGTGGCA TCAGGATTAT 
AGAAAATTCA GGGAAACAAC CATGTATCTC ATTATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT CTTCCTTATC TGTGCTTATC AGAATAACAA AGAACTGTTG 
TCCAGAGGCC CATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTACTAAAAT TAAATGAGCA AGCTGCAGAA CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA 
AACAATGGTC TGATTATCAT GAATGAATTT ATTGTCCCAT TTTTGCCCTT GTTATTGGTG 
GATGAAATGG AAGAGAAAGA CATACTTGCT GTGGAAGATA TGAGAAATCG ATGGTGTTCC 
TATCTTGGAC AAGAAATGGA ATCAAACCTC CAAGAGAAGC TGACAGATTT TCTGCCAAAG 
CTGCTTGATT GTTCTACGGA GATTAAAGGT TTCCATGAGC CGCCTAAGTT ACCTCCGTAC 
TCCACACACG AACTCTGTGA GCGATTTACC CGAATCATGC TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201215.1
CDS	128..3502
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_005201158.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201158.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACCA CAAGCCAGCC ACCTTCTAAC CAGCAAGTTG TAGAGGTGGA GATTCCTCAT 
GTAGGGACAT TTATGGTTGA GTCAAAGGAG GGGGGTTATG ATGATGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCCATA GGTAAATCCC GGAGTGTGTA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATTCAAGGTT GGTTAAATTG 
GCCCAAGAAG ATACCCCACC AGAAACAGAC TATCGTTTAC ATCATGTAAT AGTCTACTTT 
ATCCAGAATC AGGCTCCAAA GAAAATCATT GAGAAAACAT TGCTAGAACA ATTTGCAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAAATT 
GAAATGATAA AACCTGAAGA AGTAAACATG GAGGAGTATG AGGAGTGGCA TCAGGATTAT 
AGAAAATTCA GGGAAACAAC CATGTATCTC ATTATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT CTTCCTTATC TGTGCTTATC AGAATAACAA AGAACTGTTG 
TCCAGAGGCC CATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTACTAAAAT TAAATGAGCA AGCTGCAGAA CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA 
AACAATGGTC TGATTATCAT GAATGAATTT ATTGTCCCAT TTTTGCCCTT GTTATTGGTG 
GATGAAATGG AAGAGAAAGA CATACTTGCT GTGGAAGATA TGAGAAATCG ATGGTGTTCC 
TATCTTGGAC AAGAAATGGA ATCAAACCTC CAAGAGAAGC TGACAGATTT TCTGCCAAAG 
CTGCTTGATT GTTCTACGGA GATTAAAGGT TTCCATGAGC CGCCTAAGTT ACCTCCGTAC 
TCCACACACG AACTCTGTGA GCGATTTACC CGAATCATGC TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_003581669.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003581717.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

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CloneID	OBa02437
Clone ID Related Accession (Same CDS sequence)	XM_005201158.3 , XM_003581669.4 , XM_005201158.4
Accession Version	XM_003581669.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003581669.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACCA CAAGCCAGCC ACCTTCTAAC CAGCAAGTTG TAGAGGTGGA GATTCCTCAT 
GTAGGGACAT TTATGGTTGA GTCAAAGGAG GGGGGTTATG ATGATGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCCATA GGTAAATCCC GGAGTGTGTA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATTCAAGGTT GGTTAAATTG 
GCCCAAGAAG ATACCCCACC AGAAACAGAC TATCGTTTAC ATCATGTAAT AGTCTACTTT 
ATCCAGAATC AGGCTCCAAA GAAAATCATT GAGAAAACAT TGCTAGAACA ATTTGCAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAAATT 
GAAATGATAA AACCTGAAGA AGTAAACATG GAGGAGTATG AGGAGTGGCA TCAGGATTAT 
AGAAAATTCA GGGAAACAAC CATGTATCTC ATTATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT CTTCCTTATC TGTGCTTATC AGAATAACAA AGAACTGTTG 
TCCAGAGGCC CATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTACTAAAAT TAAATGAGCA AGCTGCAGAA CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA 
AACAATGGTC TGATTATCAT GAATGAATTT ATTGTCCCAT TTTTGCCCTT GTTATTGGTG 
GATGAAATGG AAGAGAAAGA CATACTTGCT GTGGAAGATA TGAGAAATCG ATGGTGTTCC 
TATCTTGGAC AAGAAATGGA ATCAAACCTC CAAGAGAAGC TGACAGATTT TCTGCCAAAG 
CTGCTTGATT GTTCTACGGA GATTAAAGGT TTCCATGAGC CGCCTAAGTT ACCTCCGTAC 
TCCACACACG AACTCTGTGA GCGATTTACC CGAATCATGC TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003581717.1
CDS	403..3777
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_003581669.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003581669.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACCA CAAGCCAGCC ACCTTCTAAC CAGCAAGTTG TAGAGGTGGA GATTCCTCAT 
GTAGGGACAT TTATGGTTGA GTCAAAGGAG GGGGGTTATG ATGATGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCCATA GGTAAATCCC GGAGTGTGTA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATTCAAGGTT GGTTAAATTG 
GCCCAAGAAG ATACCCCACC AGAAACAGAC TATCGTTTAC ATCATGTAAT AGTCTACTTT 
ATCCAGAATC AGGCTCCAAA GAAAATCATT GAGAAAACAT TGCTAGAACA ATTTGCAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAAATT 
GAAATGATAA AACCTGAAGA AGTAAACATG GAGGAGTATG AGGAGTGGCA TCAGGATTAT 
AGAAAATTCA GGGAAACAAC CATGTATCTC ATTATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT CTTCCTTATC TGTGCTTATC AGAATAACAA AGAACTGTTG 
TCCAGAGGCC CATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTACTAAAAT TAAATGAGCA AGCTGCAGAA CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA 
AACAATGGTC TGATTATCAT GAATGAATTT ATTGTCCCAT TTTTGCCCTT GTTATTGGTG 
GATGAAATGG AAGAGAAAGA CATACTTGCT GTGGAAGATA TGAGAAATCG ATGGTGTTCC 
TATCTTGGAC AAGAAATGGA ATCAAACCTC CAAGAGAAGC TGACAGATTT TCTGCCAAAG 
CTGCTTGATT GTTCTACGGA GATTAAAGGT TTCCATGAGC CGCCTAAGTT ACCTCCGTAC 
TCCACACACG AACTCTGTGA GCGATTTACC CGAATCATGC TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_005201158.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201215.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

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CloneID	OBa02437
Clone ID Related Accession (Same CDS sequence)	XM_005201158.3 , XM_003581669.4 , XM_005201158.4
Accession Version	XM_005201158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201158.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACCA CAAGCCAGCC ACCTTCTAAC CAGCAAGTTG TAGAGGTGGA GATTCCTCAT 
GTAGGGACAT TTATGGTTGA GTCAAAGGAG GGGGGTTATG ATGATGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCCATA GGTAAATCCC GGAGTGTGTA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATTCAAGGTT GGTTAAATTG 
GCCCAAGAAG ATACCCCACC AGAAACAGAC TATCGTTTAC ATCATGTAAT AGTCTACTTT 
ATCCAGAATC AGGCTCCAAA GAAAATCATT GAGAAAACAT TGCTAGAACA ATTTGCAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAAATT 
GAAATGATAA AACCTGAAGA AGTAAACATG GAGGAGTATG AGGAGTGGCA TCAGGATTAT 
AGAAAATTCA GGGAAACAAC CATGTATCTC ATTATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT CTTCCTTATC TGTGCTTATC AGAATAACAA AGAACTGTTG 
TCCAGAGGCC CATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTACTAAAAT TAAATGAGCA AGCTGCAGAA CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA 
AACAATGGTC TGATTATCAT GAATGAATTT ATTGTCCCAT TTTTGCCCTT GTTATTGGTG 
GATGAAATGG AAGAGAAAGA CATACTTGCT GTGGAAGATA TGAGAAATCG ATGGTGTTCC 
TATCTTGGAC AAGAAATGGA ATCAAACCTC CAAGAGAAGC TGACAGATTT TCTGCCAAAG 
CTGCTTGATT GTTCTACGGA GATTAAAGGT TTCCATGAGC CGCCTAAGTT ACCTCCGTAC 
TCCACACACG AACTCTGTGA GCGATTTACC CGAATCATGC TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201215.1
CDS	409..3783
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_005201158.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201158.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACCA CAAGCCAGCC ACCTTCTAAC CAGCAAGTTG TAGAGGTGGA GATTCCTCAT 
GTAGGGACAT TTATGGTTGA GTCAAAGGAG GGGGGTTATG ATGATGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCCATA GGTAAATCCC GGAGTGTGTA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATTCAAGGTT GGTTAAATTG 
GCCCAAGAAG ATACCCCACC AGAAACAGAC TATCGTTTAC ATCATGTAAT AGTCTACTTT 
ATCCAGAATC AGGCTCCAAA GAAAATCATT GAGAAAACAT TGCTAGAACA ATTTGCAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAAATT 
GAAATGATAA AACCTGAAGA AGTAAACATG GAGGAGTATG AGGAGTGGCA TCAGGATTAT 
AGAAAATTCA GGGAAACAAC CATGTATCTC ATTATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT CTTCCTTATC TGTGCTTATC AGAATAACAA AGAACTGTTG 
TCCAGAGGCC CATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTACTAAAAT TAAATGAGCA AGCTGCAGAA CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA 
AACAATGGTC TGATTATCAT GAATGAATTT ATTGTCCCAT TTTTGCCCTT GTTATTGGTG 
GATGAAATGG AAGAGAAAGA CATACTTGCT GTGGAAGATA TGAGAAATCG ATGGTGTTCC 
TATCTTGGAC AAGAAATGGA ATCAAACCTC CAAGAGAAGC TGACAGATTT TCTGCCAAAG 
CTGCTTGATT GTTCTACGGA GATTAAAGGT TTCCATGAGC CGCCTAAGTT ACCTCCGTAC 
TCCACACACG AACTCTGTGA GCGATTTACC CGAATCATGC TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_005201159.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201216.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa02438
Clone ID Related Accession (Same CDS sequence)	XM_005201159.3 , XM_005201159.4 , XM_005195959.3
Accession Version	XM_005201159.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCCATAGGTA AATCCCGGAG TGTGTATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATTC AAGGTTGGTT 
AAATTGGCCC AAGAAGATAC CCCACCAGAA ACAGACTATC GTTTACATCA TGTAATAGTC 
TACTTTATCC AGAATCAGGC TCCAAAGAAA ATCATTGAGA AAACATTGCT AGAACAATTT 
GCAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAAATTGAAA TGATAAAACC TGAAGAAGTA AACATGGAGG AGTATGAGGA GTGGCATCAG 
GATTATAGAA AATTCAGGGA AACAACCATG TATCTCATTA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTCTTC CTTATCTGTG CTTATCAGAA TAACAAAGAA 
CTGTTGTCCA GAGGCCCATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCAAGCT GCAGAACTGT TTGAATCTGG AGAGGATAGA 
GAAGTAAACA ATGGTCTGAT TATCATGAAT GAATTTATTG TCCCATTTTT GCCCTTGTTA 
TTGGTGGATG AAATGGAAGA GAAAGACATA CTTGCTGTGG AAGATATGAG AAATCGATGG 
TGTTCCTATC TTGGACAAGA AATGGAATCA AACCTCCAAG AGAAGCTGAC AGATTTTCTG 
CCAAAGCTGC TTGATTGTTC TACGGAGATT AAAGGTTTCC ATGAGCCGCC TAAGTTACCT 
CCGTACTCCA CACACGAACT CTGTGAGCGA TTTACCCGAA TCATGCTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201216.1
CDS	128..3388
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_005201159.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201159.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCCATAGGTA AATCCCGGAG TGTGTATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATTC AAGGTTGGTT 
AAATTGGCCC AAGAAGATAC CCCACCAGAA ACAGACTATC GTTTACATCA TGTAATAGTC 
TACTTTATCC AGAATCAGGC TCCAAAGAAA ATCATTGAGA AAACATTGCT AGAACAATTT 
GCAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAAATTGAAA TGATAAAACC TGAAGAAGTA AACATGGAGG AGTATGAGGA GTGGCATCAG 
GATTATAGAA AATTCAGGGA AACAACCATG TATCTCATTA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTCTTC CTTATCTGTG CTTATCAGAA TAACAAAGAA 
CTGTTGTCCA GAGGCCCATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCAAGCT GCAGAACTGT TTGAATCTGG AGAGGATAGA 
GAAGTAAACA ATGGTCTGAT TATCATGAAT GAATTTATTG TCCCATTTTT GCCCTTGTTA 
TTGGTGGATG AAATGGAAGA GAAAGACATA CTTGCTGTGG AAGATATGAG AAATCGATGG 
TGTTCCTATC TTGGACAAGA AATGGAATCA AACCTCCAAG AGAAGCTGAC AGATTTTCTG 
CCAAAGCTGC TTGATTGTTC TACGGAGATT AAAGGTTTCC ATGAGCCGCC TAAGTTACCT 
CCGTACTCCA CACACGAACT CTGTGAGCGA TTTACCCGAA TCATGCTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_005201159.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005201216.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa02438
Clone ID Related Accession (Same CDS sequence)	XM_005201159.3 , XM_005201159.4 , XM_005195959.3
Accession Version	XM_005201159.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201159.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCCATAGGTA AATCCCGGAG TGTGTATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATTC AAGGTTGGTT 
AAATTGGCCC AAGAAGATAC CCCACCAGAA ACAGACTATC GTTTACATCA TGTAATAGTC 
TACTTTATCC AGAATCAGGC TCCAAAGAAA ATCATTGAGA AAACATTGCT AGAACAATTT 
GCAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAAATTGAAA TGATAAAACC TGAAGAAGTA AACATGGAGG AGTATGAGGA GTGGCATCAG 
GATTATAGAA AATTCAGGGA AACAACCATG TATCTCATTA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTCTTC CTTATCTGTG CTTATCAGAA TAACAAAGAA 
CTGTTGTCCA GAGGCCCATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCAAGCT GCAGAACTGT TTGAATCTGG AGAGGATAGA 
GAAGTAAACA ATGGTCTGAT TATCATGAAT GAATTTATTG TCCCATTTTT GCCCTTGTTA 
TTGGTGGATG AAATGGAAGA GAAAGACATA CTTGCTGTGG AAGATATGAG AAATCGATGG 
TGTTCCTATC TTGGACAAGA AATGGAATCA AACCTCCAAG AGAAGCTGAC AGATTTTCTG 
CCAAAGCTGC TTGATTGTTC TACGGAGATT AAAGGTTTCC ATGAGCCGCC TAAGTTACCT 
CCGTACTCCA CACACGAACT CTGTGAGCGA TTTACCCGAA TCATGCTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005201216.1
CDS	408..3668
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_005201159.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005201159.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCCATAGGTA AATCCCGGAG TGTGTATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATTC AAGGTTGGTT 
AAATTGGCCC AAGAAGATAC CCCACCAGAA ACAGACTATC GTTTACATCA TGTAATAGTC 
TACTTTATCC AGAATCAGGC TCCAAAGAAA ATCATTGAGA AAACATTGCT AGAACAATTT 
GCAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAAATTGAAA TGATAAAACC TGAAGAAGTA AACATGGAGG AGTATGAGGA GTGGCATCAG 
GATTATAGAA AATTCAGGGA AACAACCATG TATCTCATTA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTCTTC CTTATCTGTG CTTATCAGAA TAACAAAGAA 
CTGTTGTCCA GAGGCCCATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCAAGCT GCAGAACTGT TTGAATCTGG AGAGGATAGA 
GAAGTAAACA ATGGTCTGAT TATCATGAAT GAATTTATTG TCCCATTTTT GCCCTTGTTA 
TTGGTGGATG AAATGGAAGA GAAAGACATA CTTGCTGTGG AAGATATGAG AAATCGATGG 
TGTTCCTATC TTGGACAAGA AATGGAATCA AACCTCCAAG AGAAGCTGAC AGATTTTCTG 
CCAAAGCTGC TTGATTGTTC TACGGAGATT AAAGGTTTCC ATGAGCCGCC TAAGTTACCT 
CCGTACTCCA CACACGAACT CTGTGAGCGA TTTACCCGAA TCATGCTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_005195959.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005196016.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa02438
Clone ID Related Accession (Same CDS sequence)	XM_005201159.3 , XM_005201159.4 , XM_005195959.3
Accession Version	XM_005195959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195959.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCCATAGGTA AATCCCGGAG TGTGTATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATTC AAGGTTGGTT 
AAATTGGCCC AAGAAGATAC CCCACCAGAA ACAGACTATC GTTTACATCA TGTAATAGTC 
TACTTTATCC AGAATCAGGC TCCAAAGAAA ATCATTGAGA AAACATTGCT AGAACAATTT 
GCAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAAATTGAAA TGATAAAACC TGAAGAAGTA AACATGGAGG AGTATGAGGA GTGGCATCAG 
GATTATAGAA AATTCAGGGA AACAACCATG TATCTCATTA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTCTTC CTTATCTGTG CTTATCAGAA TAACAAAGAA 
CTGTTGTCCA GAGGCCCATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCAAGCT GCAGAACTGT TTGAATCTGG AGAGGATAGA 
GAAGTAAACA ATGGTCTGAT TATCATGAAT GAATTTATTG TCCCATTTTT GCCCTTGTTA 
TTGGTGGATG AAATGGAAGA GAAAGACATA CTTGCTGTGG AAGATATGAG AAATCGATGG 
TGTTCCTATC TTGGACAAGA AATGGAATCA AACCTCCAAG AGAAGCTGAC AGATTTTCTG 
CCAAAGCTGC TTGATTGTTC TACGGAGATT AAAGGTTTCC ATGAGCCGCC TAAGTTACCT 
CCGTACTCCA CACACGAACT CTGTGAGCGA TTTACCCGAA TCATGCTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005196016.1
CDS	403..3663
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_005195959.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195959.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCCATAGGTA AATCCCGGAG TGTGTATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATTC AAGGTTGGTT 
AAATTGGCCC AAGAAGATAC CCCACCAGAA ACAGACTATC GTTTACATCA TGTAATAGTC 
TACTTTATCC AGAATCAGGC TCCAAAGAAA ATCATTGAGA AAACATTGCT AGAACAATTT 
GCAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAAATTGAAA TGATAAAACC TGAAGAAGTA AACATGGAGG AGTATGAGGA GTGGCATCAG 
GATTATAGAA AATTCAGGGA AACAACCATG TATCTCATTA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTCTTC CTTATCTGTG CTTATCAGAA TAACAAAGAA 
CTGTTGTCCA GAGGCCCATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTAC TAAAATTAAA TGAGCAAGCT GCAGAACTGT TTGAATCTGG AGAGGATAGA 
GAAGTAAACA ATGGTCTGAT TATCATGAAT GAATTTATTG TCCCATTTTT GCCCTTGTTA 
TTGGTGGATG AAATGGAAGA GAAAGACATA CTTGCTGTGG AAGATATGAG AAATCGATGG 
TGTTCCTATC TTGGACAAGA AATGGAATCA AACCTCCAAG AGAAGCTGAC AGATTTTCTG 
CCAAAGCTGC TTGATTGTTC TACGGAGATT AAAGGTTTCC ATGAGCCGCC TAAGTTACCT 
CCGTACTCCA CACACGAACT CTGTGAGCGA TTTACCCGAA TCATGCTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_602857.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_602857.5 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

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CloneID	OBa02439
Clone ID Related Accession (Same CDS sequence)	XM_602857.8 , XM_002684646.5 , XM_002684646.6
Accession Version	XM_602857.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_602857.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_602857.5
CDS	403..3567
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_602857.8
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_602857.8

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_002684646.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684692.2 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa02439
Clone ID Related Accession (Same CDS sequence)	XM_602857.8 , XM_002684646.5 , XM_002684646.6
Accession Version	XM_002684646.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002684646.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684692.2
CDS	128..3292
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_002684646.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684646.5

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_002684646.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684692.2 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa02439
Clone ID Related Accession (Same CDS sequence)	XM_602857.8 , XM_002684646.5 , XM_002684646.6
Accession Version	XM_002684646.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002684646.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684692.2
CDS	407..3571
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_002684646.6
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684646.6

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAAT GTGATTGATC TTACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCGGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAGCA AGCCATTAGC AGAGTTCTTG AGGCCAGCAT AGCAGAAAAT 
AAAGCATGTT TGAAGAGGAC ACCTACTGAA GTTTGGAGAG ATTCTCGAAA CCCTTACGAT 
AGAAAAAGGC AGGACAAAGC TCCTGTTGGA CTAAAGAATG TTGGCAATAC TTGCTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTCAATCTT TTGGAATTCA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGTTCA AGATTTACCC CGAAACCAAA AGGAGCATCG GAATTTGCCT 
TTTATGCGTG AGCTTCGATA TCTATTTGCA CTTCTTGTTG GTACTAAAAG GAAATATGTT 
GATCCATCAA GAGCAGTTGA GATTCTTAAA GATGCTTTCA AATCAAATGA CTCCCAGCAG 
CAAGACGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AAGAGACGGA TGAAGACAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGAAGATTCC TAGCTGTGGG AGTGCTTGAA GGTAAAAAAT TTGAAAATAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGATTT AAAGATCTAC ATGAATGCCT AGAAGCTGCT 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAAAATT CAGGAAAATC TGGTCAAGAG 
CATTGGTTTA CTGAACTCCC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCACTGG GAAGACCAGA AAAAATTCAC AATAAATTAG AATTTCCTCA AGTTTTATAT 
CTGGACAGAT ACATGCATAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTAAAAG ATTACCTCAC AGTATTACAA CAAAGACTTG AAAGGTATTT AAGCTATGGT 
TCGGGTCCCA AACGGTTTCC CTTGGTGGAT GTTCTGCAGT ACGCCTTAGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAT GATATTGATG CTAGTTCCCC ATCTAGTGGT 
TCCGTACCAT CACAGACATT ACCAAGCACA ACAGACCAAC AGGGAGCTCC TTCTTCAGAA 
CTGCCAAGCA CGTCACCCGC GTCAATAGCT GCCATTTCAT CAAGATCAGT AATACACAAA 
CCATTCACTC AGTCTCGGAT ACCTCCAGAC TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACAGAAGAAG AACTCTCTGT GCTAGAAAGC TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTACA GGAAAGCATA TCTAGAATCC ATCGCACAAT TGAGCTAATG 
TACTCTGACA AATCTATGAT CCAAGTTCCT TATCGTTTAC ATGCTGTTTT AGTTCATGAA 
GGTCAAGCTA ATGCCGGGCA CTACTGGGCA TACATTTTTG ATCATCGTGA AAACAGGTGG 
ATGAAGTACA ATGATATTGC TGTAACAAAA TCATCATGGG AAGAGCTAGT GAGGGATTCT 
TTTGGTGGTT ACAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA CAAACCACAA 
TTCTTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGG CCCTTGTTGG AATAGAAACA 
CTACCCCCTG ACTTAAGAGA TTTTGTTGAG GAAGACAACC AGCGATTTGA GAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCTCAGAAA GCCTTGCAGG AAAAACTTCT AGCATCTCAG 
AAATTGAGAG AGTCCGAGTC TTCTGTGACA ACAGCACAAG CAGGAGAACC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACCAT TCGAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010800945.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799247.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

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CloneID	OBa50891
Clone ID Related Accession (Same CDS sequence)	XM_010800945.2 , XM_010824431.2 , XM_010800945.3
Accession Version	XM_010800945.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAA ACCTGAAAAT ACCACAAGCC AGCCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGAGATTCC TCATGTAGGG ACATTTATGG TTGAGTCAAA GGAGGGGGGT 
TATGATGATG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC CATAGGTAAA 
TCCCGGAGTG TGTATGACAG GTGTGGCCCT GAAGCAGGGT TCTTTAAGGC AATTAAGTTG 
GAATATTCAA GGTTGGTTAA ATTGGCCCAA GAAGATACCC CACCAGAAAC AGACTATCGT 
TTACATCATG TAATAGTCTA CTTTATCCAG AATCAGGCTC CAAAGAAAAT CATTGAGAAA 
ACATTGCTAG AACAATTTGC AGATAGAAAT TTGAGTTTTG ATGAAAGGTG TCACAACATA 
ATGAAAGTTG CTCAAGCCAA AATTGAAATG ATAAAACCTG AAGAAGTAAA CATGGAGGAG 
TATGAGGAGT GGCATCAGGA TTATAGAAAA TTCAGGGAAA CAACCATGTA TCTCATTATT 
GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT ATAGATTCCT TGCTCTTCCT TATCTGTGCT 
TATCAGAATA ACAAAGAACT GTTGTCCAGA GGCCCATACA GAGGACATGA TGAAGAATTG 
ATATCACATT ATAGAAGAGA ATGTTTACTA AAATTAAATG AGCAAGCTGC AGAACTGTTT 
GAATCTGGAG AGGATAGAGA AGTAAACAAT GGTCTGATTA TCATGAATGA ATTTATTGTC 
CCATTTTTGC CCTTGTTATT GGTGGATGAA ATGGAAGAGA AAGACATACT TGCTGTGGAA 
GATATGAGAA ATCGATGGTG TTCCTATCTT GGACAAGAAA TGGAATCAAA CCTCCAAGAG 
AAGCTGACAG ATTTTCTGCC AAAGCTGCTT GATTGTTCTA CGGAGATTAA AGGTTTCCAT 
GAGCCGCCTA AGTTACCTCC GTACTCCACA CACGAACTCT GTGAGCGATT TACCCGAATC 
ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799247.1
CDS	128..3586
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010800945.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800945.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAA ACCTGAAAAT ACCACAAGCC AGCCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGAGATTCC TCATGTAGGG ACATTTATGG TTGAGTCAAA GGAGGGGGGT 
TATGATGATG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC CATAGGTAAA 
TCCCGGAGTG TGTATGACAG GTGTGGCCCT GAAGCAGGGT TCTTTAAGGC AATTAAGTTG 
GAATATTCAA GGTTGGTTAA ATTGGCCCAA GAAGATACCC CACCAGAAAC AGACTATCGT 
TTACATCATG TAATAGTCTA CTTTATCCAG AATCAGGCTC CAAAGAAAAT CATTGAGAAA 
ACATTGCTAG AACAATTTGC AGATAGAAAT TTGAGTTTTG ATGAAAGGTG TCACAACATA 
ATGAAAGTTG CTCAAGCCAA AATTGAAATG ATAAAACCTG AAGAAGTAAA CATGGAGGAG 
TATGAGGAGT GGCATCAGGA TTATAGAAAA TTCAGGGAAA CAACCATGTA TCTCATTATT 
GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT ATAGATTCCT TGCTCTTCCT TATCTGTGCT 
TATCAGAATA ACAAAGAACT GTTGTCCAGA GGCCCATACA GAGGACATGA TGAAGAATTG 
ATATCACATT ATAGAAGAGA ATGTTTACTA AAATTAAATG AGCAAGCTGC AGAACTGTTT 
GAATCTGGAG AGGATAGAGA AGTAAACAAT GGTCTGATTA TCATGAATGA ATTTATTGTC 
CCATTTTTGC CCTTGTTATT GGTGGATGAA ATGGAAGAGA AAGACATACT TGCTGTGGAA 
GATATGAGAA ATCGATGGTG TTCCTATCTT GGACAAGAAA TGGAATCAAA CCTCCAAGAG 
AAGCTGACAG ATTTTCTGCC AAAGCTGCTT GATTGTTCTA CGGAGATTAA AGGTTTCCAT 
GAGCCGCCTA AGTTACCTCC GTACTCCACA CACGAACTCT GTGAGCGATT TACCCGAATC 
ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010824431.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822733.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa50891
Clone ID Related Accession (Same CDS sequence)	XM_010800945.2 , XM_010824431.2 , XM_010800945.3
Accession Version	XM_010824431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAA ACCTGAAAAT ACCACAAGCC AGCCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGAGATTCC TCATGTAGGG ACATTTATGG TTGAGTCAAA GGAGGGGGGT 
TATGATGATG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC CATAGGTAAA 
TCCCGGAGTG TGTATGACAG GTGTGGCCCT GAAGCAGGGT TCTTTAAGGC AATTAAGTTG 
GAATATTCAA GGTTGGTTAA ATTGGCCCAA GAAGATACCC CACCAGAAAC AGACTATCGT 
TTACATCATG TAATAGTCTA CTTTATCCAG AATCAGGCTC CAAAGAAAAT CATTGAGAAA 
ACATTGCTAG AACAATTTGC AGATAGAAAT TTGAGTTTTG ATGAAAGGTG TCACAACATA 
ATGAAAGTTG CTCAAGCCAA AATTGAAATG ATAAAACCTG AAGAAGTAAA CATGGAGGAG 
TATGAGGAGT GGCATCAGGA TTATAGAAAA TTCAGGGAAA CAACCATGTA TCTCATTATT 
GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT ATAGATTCCT TGCTCTTCCT TATCTGTGCT 
TATCAGAATA ACAAAGAACT GTTGTCCAGA GGCCCATACA GAGGACATGA TGAAGAATTG 
ATATCACATT ATAGAAGAGA ATGTTTACTA AAATTAAATG AGCAAGCTGC AGAACTGTTT 
GAATCTGGAG AGGATAGAGA AGTAAACAAT GGTCTGATTA TCATGAATGA ATTTATTGTC 
CCATTTTTGC CCTTGTTATT GGTGGATGAA ATGGAAGAGA AAGACATACT TGCTGTGGAA 
GATATGAGAA ATCGATGGTG TTCCTATCTT GGACAAGAAA TGGAATCAAA CCTCCAAGAG 
AAGCTGACAG ATTTTCTGCC AAAGCTGCTT GATTGTTCTA CGGAGATTAA AGGTTTCCAT 
GAGCCGCCTA AGTTACCTCC GTACTCCACA CACGAACTCT GTGAGCGATT TACCCGAATC 
ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822733.1
CDS	403..3861
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010824431.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824431.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAA ACCTGAAAAT ACCACAAGCC AGCCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGAGATTCC TCATGTAGGG ACATTTATGG TTGAGTCAAA GGAGGGGGGT 
TATGATGATG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC CATAGGTAAA 
TCCCGGAGTG TGTATGACAG GTGTGGCCCT GAAGCAGGGT TCTTTAAGGC AATTAAGTTG 
GAATATTCAA GGTTGGTTAA ATTGGCCCAA GAAGATACCC CACCAGAAAC AGACTATCGT 
TTACATCATG TAATAGTCTA CTTTATCCAG AATCAGGCTC CAAAGAAAAT CATTGAGAAA 
ACATTGCTAG AACAATTTGC AGATAGAAAT TTGAGTTTTG ATGAAAGGTG TCACAACATA 
ATGAAAGTTG CTCAAGCCAA AATTGAAATG ATAAAACCTG AAGAAGTAAA CATGGAGGAG 
TATGAGGAGT GGCATCAGGA TTATAGAAAA TTCAGGGAAA CAACCATGTA TCTCATTATT 
GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT ATAGATTCCT TGCTCTTCCT TATCTGTGCT 
TATCAGAATA ACAAAGAACT GTTGTCCAGA GGCCCATACA GAGGACATGA TGAAGAATTG 
ATATCACATT ATAGAAGAGA ATGTTTACTA AAATTAAATG AGCAAGCTGC AGAACTGTTT 
GAATCTGGAG AGGATAGAGA AGTAAACAAT GGTCTGATTA TCATGAATGA ATTTATTGTC 
CCATTTTTGC CCTTGTTATT GGTGGATGAA ATGGAAGAGA AAGACATACT TGCTGTGGAA 
GATATGAGAA ATCGATGGTG TTCCTATCTT GGACAAGAAA TGGAATCAAA CCTCCAAGAG 
AAGCTGACAG ATTTTCTGCC AAAGCTGCTT GATTGTTCTA CGGAGATTAA AGGTTTCCAT 
GAGCCGCCTA AGTTACCTCC GTACTCCACA CACGAACTCT GTGAGCGATT TACCCGAATC 
ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010800945.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799247.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

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CloneID	OBa50891
Clone ID Related Accession (Same CDS sequence)	XM_010800945.2 , XM_010824431.2 , XM_010800945.3
Accession Version	XM_010800945.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAA ACCTGAAAAT ACCACAAGCC AGCCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGAGATTCC TCATGTAGGG ACATTTATGG TTGAGTCAAA GGAGGGGGGT 
TATGATGATG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC CATAGGTAAA 
TCCCGGAGTG TGTATGACAG GTGTGGCCCT GAAGCAGGGT TCTTTAAGGC AATTAAGTTG 
GAATATTCAA GGTTGGTTAA ATTGGCCCAA GAAGATACCC CACCAGAAAC AGACTATCGT 
TTACATCATG TAATAGTCTA CTTTATCCAG AATCAGGCTC CAAAGAAAAT CATTGAGAAA 
ACATTGCTAG AACAATTTGC AGATAGAAAT TTGAGTTTTG ATGAAAGGTG TCACAACATA 
ATGAAAGTTG CTCAAGCCAA AATTGAAATG ATAAAACCTG AAGAAGTAAA CATGGAGGAG 
TATGAGGAGT GGCATCAGGA TTATAGAAAA TTCAGGGAAA CAACCATGTA TCTCATTATT 
GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT ATAGATTCCT TGCTCTTCCT TATCTGTGCT 
TATCAGAATA ACAAAGAACT GTTGTCCAGA GGCCCATACA GAGGACATGA TGAAGAATTG 
ATATCACATT ATAGAAGAGA ATGTTTACTA AAATTAAATG AGCAAGCTGC AGAACTGTTT 
GAATCTGGAG AGGATAGAGA AGTAAACAAT GGTCTGATTA TCATGAATGA ATTTATTGTC 
CCATTTTTGC CCTTGTTATT GGTGGATGAA ATGGAAGAGA AAGACATACT TGCTGTGGAA 
GATATGAGAA ATCGATGGTG TTCCTATCTT GGACAAGAAA TGGAATCAAA CCTCCAAGAG 
AAGCTGACAG ATTTTCTGCC AAAGCTGCTT GATTGTTCTA CGGAGATTAA AGGTTTCCAT 
GAGCCGCCTA AGTTACCTCC GTACTCCACA CACGAACTCT GTGAGCGATT TACCCGAATC 
ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799247.1
CDS	409..3867
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSKPENTTSQPPSNQQVVEVEIPHVGTFMVESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010800945.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800945.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAA ACCTGAAAAT ACCACAAGCC AGCCACCTTC TAACCAGCAA 
GTTGTAGAGG TGGAGATTCC TCATGTAGGG ACATTTATGG TTGAGTCAAA GGAGGGGGGT 
TATGATGATG AGATCATGAT GACACCGAAC ATGCAAGGTA TTATCATGGC CATAGGTAAA 
TCCCGGAGTG TGTATGACAG GTGTGGCCCT GAAGCAGGGT TCTTTAAGGC AATTAAGTTG 
GAATATTCAA GGTTGGTTAA ATTGGCCCAA GAAGATACCC CACCAGAAAC AGACTATCGT 
TTACATCATG TAATAGTCTA CTTTATCCAG AATCAGGCTC CAAAGAAAAT CATTGAGAAA 
ACATTGCTAG AACAATTTGC AGATAGAAAT TTGAGTTTTG ATGAAAGGTG TCACAACATA 
ATGAAAGTTG CTCAAGCCAA AATTGAAATG ATAAAACCTG AAGAAGTAAA CATGGAGGAG 
TATGAGGAGT GGCATCAGGA TTATAGAAAA TTCAGGGAAA CAACCATGTA TCTCATTATT 
GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT ATAGATTCCT TGCTCTTCCT TATCTGTGCT 
TATCAGAATA ACAAAGAACT GTTGTCCAGA GGCCCATACA GAGGACATGA TGAAGAATTG 
ATATCACATT ATAGAAGAGA ATGTTTACTA AAATTAAATG AGCAAGCTGC AGAACTGTTT 
GAATCTGGAG AGGATAGAGA AGTAAACAAT GGTCTGATTA TCATGAATGA ATTTATTGTC 
CCATTTTTGC CCTTGTTATT GGTGGATGAA ATGGAAGAGA AAGACATACT TGCTGTGGAA 
GATATGAGAA ATCGATGGTG TTCCTATCTT GGACAAGAAA TGGAATCAAA CCTCCAAGAG 
AAGCTGACAG ATTTTCTGCC AAAGCTGCTT GATTGTTCTA CGGAGATTAA AGGTTTCCAT 
GAGCCGCCTA AGTTACCTCC GTACTCCACA CACGAACTCT GTGAGCGATT TACCCGAATC 
ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010800946.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799248.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

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CloneID	OBa50892
Clone ID Related Accession (Same CDS sequence)	XM_010800946.3 , XM_010800946.2 , XM_010824432.2
Accession Version	XM_010800946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAT CATGATGACA CCGAACATGC AAGGTATTAT CATGGCCATA 
GGTAAATCCC GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799248.1
CDS	408..3752
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010800946.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800946.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAT CATGATGACA CCGAACATGC AAGGTATTAT CATGGCCATA 
GGTAAATCCC GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010800946.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799248.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

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CloneID	OBa50892
Clone ID Related Accession (Same CDS sequence)	XM_010800946.3 , XM_010800946.2 , XM_010824432.2
Accession Version	XM_010800946.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAT CATGATGACA CCGAACATGC AAGGTATTAT CATGGCCATA 
GGTAAATCCC GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799248.1
CDS	128..3472
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010800946.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800946.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAT CATGATGACA CCGAACATGC AAGGTATTAT CATGGCCATA 
GGTAAATCCC GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010824432.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822734.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

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CloneID	OBa50892
Clone ID Related Accession (Same CDS sequence)	XM_010800946.3 , XM_010800946.2 , XM_010824432.2
Accession Version	XM_010824432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAT CATGATGACA CCGAACATGC AAGGTATTAT CATGGCCATA 
GGTAAATCCC GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822734.1
CDS	403..3747
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010824432.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824432.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGAT CATGATGACA CCGAACATGC AAGGTATTAT CATGGCCATA 
GGTAAATCCC GGAGTGTGTA TGACAGGTGT GGCCCTGAAG CAGGGTTCTT TAAGGCAATT 
AAGTTGGAAT ATTCAAGGTT GGTTAAATTG GCCCAAGAAG ATACCCCACC AGAAACAGAC 
TATCGTTTAC ATCATGTAAT AGTCTACTTT ATCCAGAATC AGGCTCCAAA GAAAATCATT 
GAGAAAACAT TGCTAGAACA ATTTGCAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAAATT GAAATGATAA AACCTGAAGA AGTAAACATG 
GAGGAGTATG AGGAGTGGCA TCAGGATTAT AGAAAATTCA GGGAAACAAC CATGTATCTC 
ATTATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT CTTCCTTATC 
TGTGCTTATC AGAATAACAA AGAACTGTTG TCCAGAGGCC CATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTACTAAAAT TAAATGAGCA AGCTGCAGAA 
CTGTTTGAAT CTGGAGAGGA TAGAGAAGTA AACAATGGTC TGATTATCAT GAATGAATTT 
ATTGTCCCAT TTTTGCCCTT GTTATTGGTG GATGAAATGG AAGAGAAAGA CATACTTGCT 
GTGGAAGATA TGAGAAATCG ATGGTGTTCC TATCTTGGAC AAGAAATGGA ATCAAACCTC 
CAAGAGAAGC TGACAGATTT TCTGCCAAAG CTGCTTGATT GTTCTACGGA GATTAAAGGT 
TTCCATGAGC CGCCTAAGTT ACCTCCGTAC TCCACACACG AACTCTGTGA GCGATTTACC 
CGAATCATGC TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010824433.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822735.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa50893
Clone ID Related Accession (Same CDS sequence)	XM_010824433.2 , XM_010800947.2 , XM_010800947.3
Accession Version	XM_010824433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGGC AATTAAGTTG GAATATTCAA GGTTGGTTAA ATTGGCCCAA 
GAAGATACCC CACCAGAAAC AGACTATCGT TTACATCATG TAATAGTCTA CTTTATCCAG 
AATCAGGCTC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGC AGATAGAAAT 
TTGAGTTTTG ATGAAAGGTG TCACAACATA ATGAAAGTTG CTCAAGCCAA AATTGAAATG 
ATAAAACCTG AAGAAGTAAA CATGGAGGAG TATGAGGAGT GGCATCAGGA TTATAGAAAA 
TTCAGGGAAA CAACCATGTA TCTCATTATT GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT 
ATAGATTCCT TGCTCTTCCT TATCTGTGCT TATCAGAATA ACAAAGAACT GTTGTCCAGA 
GGCCCATACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA 
AAATTAAATG AGCAAGCTGC AGAACTGTTT GAATCTGGAG AGGATAGAGA AGTAAACAAT 
GGTCTGATTA TCATGAATGA ATTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA 
ATGGAAGAGA AAGACATACT TGCTGTGGAA GATATGAGAA ATCGATGGTG TTCCTATCTT 
GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAGCTGCTT 
GATTGTTCTA CGGAGATTAA AGGTTTCCAT GAGCCGCCTA AGTTACCTCC GTACTCCACA 
CACGAACTCT GTGAGCGATT TACCCGAATC ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822735.1
CDS	403..3651
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010824433.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824433.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGGC AATTAAGTTG GAATATTCAA GGTTGGTTAA ATTGGCCCAA 
GAAGATACCC CACCAGAAAC AGACTATCGT TTACATCATG TAATAGTCTA CTTTATCCAG 
AATCAGGCTC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGC AGATAGAAAT 
TTGAGTTTTG ATGAAAGGTG TCACAACATA ATGAAAGTTG CTCAAGCCAA AATTGAAATG 
ATAAAACCTG AAGAAGTAAA CATGGAGGAG TATGAGGAGT GGCATCAGGA TTATAGAAAA 
TTCAGGGAAA CAACCATGTA TCTCATTATT GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT 
ATAGATTCCT TGCTCTTCCT TATCTGTGCT TATCAGAATA ACAAAGAACT GTTGTCCAGA 
GGCCCATACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA 
AAATTAAATG AGCAAGCTGC AGAACTGTTT GAATCTGGAG AGGATAGAGA AGTAAACAAT 
GGTCTGATTA TCATGAATGA ATTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA 
ATGGAAGAGA AAGACATACT TGCTGTGGAA GATATGAGAA ATCGATGGTG TTCCTATCTT 
GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAGCTGCTT 
GATTGTTCTA CGGAGATTAA AGGTTTCCAT GAGCCGCCTA AGTTACCTCC GTACTCCACA 
CACGAACTCT GTGAGCGATT TACCCGAATC ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010800947.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799249.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa50893
Clone ID Related Accession (Same CDS sequence)	XM_010824433.2 , XM_010800947.2 , XM_010800947.3
Accession Version	XM_010800947.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGGC AATTAAGTTG GAATATTCAA GGTTGGTTAA ATTGGCCCAA 
GAAGATACCC CACCAGAAAC AGACTATCGT TTACATCATG TAATAGTCTA CTTTATCCAG 
AATCAGGCTC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGC AGATAGAAAT 
TTGAGTTTTG ATGAAAGGTG TCACAACATA ATGAAAGTTG CTCAAGCCAA AATTGAAATG 
ATAAAACCTG AAGAAGTAAA CATGGAGGAG TATGAGGAGT GGCATCAGGA TTATAGAAAA 
TTCAGGGAAA CAACCATGTA TCTCATTATT GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT 
ATAGATTCCT TGCTCTTCCT TATCTGTGCT TATCAGAATA ACAAAGAACT GTTGTCCAGA 
GGCCCATACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA 
AAATTAAATG AGCAAGCTGC AGAACTGTTT GAATCTGGAG AGGATAGAGA AGTAAACAAT 
GGTCTGATTA TCATGAATGA ATTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA 
ATGGAAGAGA AAGACATACT TGCTGTGGAA GATATGAGAA ATCGATGGTG TTCCTATCTT 
GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAGCTGCTT 
GATTGTTCTA CGGAGATTAA AGGTTTCCAT GAGCCGCCTA AGTTACCTCC GTACTCCACA 
CACGAACTCT GTGAGCGATT TACCCGAATC ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799249.1
CDS	128..3376
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010800947.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800947.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGGC AATTAAGTTG GAATATTCAA GGTTGGTTAA ATTGGCCCAA 
GAAGATACCC CACCAGAAAC AGACTATCGT TTACATCATG TAATAGTCTA CTTTATCCAG 
AATCAGGCTC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGC AGATAGAAAT 
TTGAGTTTTG ATGAAAGGTG TCACAACATA ATGAAAGTTG CTCAAGCCAA AATTGAAATG 
ATAAAACCTG AAGAAGTAAA CATGGAGGAG TATGAGGAGT GGCATCAGGA TTATAGAAAA 
TTCAGGGAAA CAACCATGTA TCTCATTATT GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT 
ATAGATTCCT TGCTCTTCCT TATCTGTGCT TATCAGAATA ACAAAGAACT GTTGTCCAGA 
GGCCCATACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA 
AAATTAAATG AGCAAGCTGC AGAACTGTTT GAATCTGGAG AGGATAGAGA AGTAAACAAT 
GGTCTGATTA TCATGAATGA ATTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA 
ATGGAAGAGA AAGACATACT TGCTGTGGAA GATATGAGAA ATCGATGGTG TTCCTATCTT 
GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAGCTGCTT 
GATTGTTCTA CGGAGATTAA AGGTTTCCAT GAGCCGCCTA AGTTACCTCC GTACTCCACA 
CACGAACTCT GTGAGCGATT TACCCGAATC ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_010800947.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799249.1 Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa50893
Clone ID Related Accession (Same CDS sequence)	XM_010824433.2 , XM_010800947.2 , XM_010800947.3
Accession Version	XM_010800947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGGC AATTAAGTTG GAATATTCAA GGTTGGTTAA ATTGGCCCAA 
GAAGATACCC CACCAGAAAC AGACTATCGT TTACATCATG TAATAGTCTA CTTTATCCAG 
AATCAGGCTC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGC AGATAGAAAT 
TTGAGTTTTG ATGAAAGGTG TCACAACATA ATGAAAGTTG CTCAAGCCAA AATTGAAATG 
ATAAAACCTG AAGAAGTAAA CATGGAGGAG TATGAGGAGT GGCATCAGGA TTATAGAAAA 
TTCAGGGAAA CAACCATGTA TCTCATTATT GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT 
ATAGATTCCT TGCTCTTCCT TATCTGTGCT TATCAGAATA ACAAAGAACT GTTGTCCAGA 
GGCCCATACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA 
AAATTAAATG AGCAAGCTGC AGAACTGTTT GAATCTGGAG AGGATAGAGA AGTAAACAAT 
GGTCTGATTA TCATGAATGA ATTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA 
ATGGAAGAGA AAGACATACT TGCTGTGGAA GATATGAGAA ATCGATGGTG TTCCTATCTT 
GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAGCTGCTT 
GATTGTTCTA CGGAGATTAA AGGTTTCCAT GAGCCGCCTA AGTTACCTCC GTACTCCACA 
CACGAACTCT GTGAGCGATT TACCCGAATC ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799249.1
CDS	407..3655
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDAQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTRKKQYMPTRTRCATIEQSKYGSKDKRDLDVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNVQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEDKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPSSGSVPSQTLPSTTDQQGAPSSELPSTSPASIAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKPQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESESSVTTAQAGEPEYLEQPSRSDFSKHLKEETIRIITKASHEHEDKSPETVLQSAIKLEYSRLVKLAQEDTPPETDYRLHHVIVYFIQNQAPKKIIEKTLLEQFADRNLSFDERCHNIMKVAQAKIEMIKPEEVNMEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSRGPYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPPYSTHELCERFTRIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_010800947.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin specific peptidase 25 (USP25), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800947.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
CTGAATCAAC TACGAGAAAT TACGGGGATT AATGATGCCC AGATTCTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAGCTAGCA GTGGCTTTCC TTACTGCAAA GAATGCCAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCGC TTCCTGGCAA TGACAGATAC 
ATCAGTGTGG GCAGCCAAGC GGACACAAGA AAAAAACAGT ATATGCCCAC CAGAACAAGA 
TGTGCTACAA TTGAGCAATC AAAGTATGGA TCAAAAGATA AGAGGGATTT GGATGTGATT 
GATCTTACTG GAGATGATAA AGATGATCTT CAGAGAGCAA TTGCCTTGAG TTTGGCGGAA 
TCAAACAGGG CATTCAGGGA GACTGGAATA ACTGATGAGG AGCAAGCCAT TAGCAGAGTT 
CTTGAGGCCA GCATAGCAGA AAATAAAGCA TGTTTGAAGA GGACACCTAC TGAAGTTTGG 
AGAGATTCTC GAAACCCTTA CGATAGAAAA AGGCAGGACA AAGCTCCTGT TGGACTAAAG 
AATGTTGGCA ATACTTGCTG GTTTAGTGCT GTTATTCAGT CATTATTCAA TCTTTTGGAA 
TTCAGAAGAT TAGTTCTGAA TTACAAGCCT CCATCAAATG TTCAAGATTT ACCCCGAAAC 
CAAAAGGAGC ATCGGAATTT GCCTTTTATG CGTGAGCTTC GATATCTATT TGCACTTCTT 
GTTGGTACTA AAAGGAAATA TGTTGATCCA TCAAGAGCAG TTGAGATTCT TAAAGATGCT 
TTCAAATCAA ATGACTCCCA GCAGCAAGAC GTGAGTGAGT TTACACACAA ATTATTAGAT 
TGGTTAGAAG ATGCCTTCCA AATGAAAGCT GAAGAAGAGA CGGATGAAGA CAAGCCAAAG 
AACCCCATGG TAGAGTTGTT CTATGGAAGA TTCCTAGCTG TGGGAGTGCT TGAAGGTAAA 
AAATTTGAAA ATACTGAAAT GTTTGGTCAG TACCCACTTC AGGTCAATGG ATTTAAAGAT 
CTACATGAAT GCCTAGAAGC TGCTATGATT GAAGGAGAAA TTGAGTCTTT ACATTCAGAA 
AATTCAGGAA AATCTGGTCA AGAGCATTGG TTTACTGAAC TCCCACCTGT GTTAACATTT 
GAATTGTCAA GATTTGAATT TAATCAGGCA CTGGGAAGAC CAGAAAAAAT TCACAATAAA 
TTAGAATTTC CTCAAGTTTT ATATCTGGAC AGATACATGC ATAGAAACAG AGAAATAACA 
AGAATTAAGA GGGAAGAGAT CAAGAGACTA AAAGATTACC TCACAGTATT ACAACAAAGA 
CTTGAAAGGT ATTTAAGCTA TGGTTCGGGT CCCAAACGGT TTCCCTTGGT GGATGTTCTG 
CAGTACGCCT TAGAATTTGC CTCAAGTAAA CCTGTTTGCA CTTCTCCTGT TGATGATATT 
GATGCTAGTT CCCCATCTAG TGGTTCCGTA CCATCACAGA CATTACCAAG CACAACAGAC 
CAACAGGGAG CTCCTTCTTC AGAACTGCCA AGCACGTCAC CCGCGTCAAT AGCTGCCATT 
TCATCAAGAT CAGTAATACA CAAACCATTC ACTCAGTCTC GGATACCTCC AGACTTGCCC 
ATGCATCCGG CACCAAGGCA CATAACAGAA GAAGAACTCT CTGTGCTAGA AAGCTGTTTA 
CATCGCTGGA GGACAGAAAT AGAAAATGAC ACCAGAGATT TACAGGAAAG CATATCTAGA 
ATCCATCGCA CAATTGAGCT AATGTACTCT GACAAATCTA TGATCCAAGT TCCTTATCGT 
TTACATGCTG TTTTAGTTCA TGAAGGTCAA GCTAATGCCG GGCACTACTG GGCATACATT 
TTTGATCATC GTGAAAACAG GTGGATGAAG TACAATGATA TTGCTGTAAC AAAATCATCA 
TGGGAAGAGC TAGTGAGGGA TTCTTTTGGT GGTTACAGAA ATGCCAGTGC ATACTGTTTA 
ATGTACATAA ATGACAAACC ACAATTCTTA ATACAAGAGG AGTTTAATAA AGAAACTGGG 
CAGGCCCTTG TTGGAATAGA AACACTACCC CCTGACTTAA GAGATTTTGT TGAGGAAGAC 
AACCAGCGAT TTGAGAAAGA ACTAGAAGAA TGGGATGCAC AACTTGCTCA GAAAGCCTTG 
CAGGAAAAAC TTCTAGCATC TCAGAAATTG AGAGAGTCCG AGTCTTCTGT GACAACAGCA 
CAAGCAGGAG AACCAGAATA TCTAGAGCAG CCATCAAGAA GTGATTTCTC AAAGCACTTG 
AAAGAAGAAA CCATTCGAAT AATTACCAAG GCATCACATG AGCATGAAGA TAAAAGTCCT 
GAAACAGTTT TGCAGTCGGC AATTAAGTTG GAATATTCAA GGTTGGTTAA ATTGGCCCAA 
GAAGATACCC CACCAGAAAC AGACTATCGT TTACATCATG TAATAGTCTA CTTTATCCAG 
AATCAGGCTC CAAAGAAAAT CATTGAGAAA ACATTGCTAG AACAATTTGC AGATAGAAAT 
TTGAGTTTTG ATGAAAGGTG TCACAACATA ATGAAAGTTG CTCAAGCCAA AATTGAAATG 
ATAAAACCTG AAGAAGTAAA CATGGAGGAG TATGAGGAGT GGCATCAGGA TTATAGAAAA 
TTCAGGGAAA CAACCATGTA TCTCATTATT GGGCTAGAAA ATTTTCAAAG AGAAAGTTAT 
ATAGATTCCT TGCTCTTCCT TATCTGTGCT TATCAGAATA ACAAAGAACT GTTGTCCAGA 
GGCCCATACA GAGGACATGA TGAAGAATTG ATATCACATT ATAGAAGAGA ATGTTTACTA 
AAATTAAATG AGCAAGCTGC AGAACTGTTT GAATCTGGAG AGGATAGAGA AGTAAACAAT 
GGTCTGATTA TCATGAATGA ATTTATTGTC CCATTTTTGC CCTTGTTATT GGTGGATGAA 
ATGGAAGAGA AAGACATACT TGCTGTGGAA GATATGAGAA ATCGATGGTG TTCCTATCTT 
GGACAAGAAA TGGAATCAAA CCTCCAAGAG AAGCTGACAG ATTTTCTGCC AAAGCTGCTT 
GATTGTTCTA CGGAGATTAA AGGTTTCCAT GAGCCGCCTA AGTTACCTCC GTACTCCACA 
CACGAACTCT GTGAGCGATT TACCCGAATC ATGCTGTCCC TCAGTCGAAC TCCTGCTGAT 
GGAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	USP25
Entrez Gene ID	524531
Full Name	ubiquitin specific peptidase 25
General protein information	Preferred Names ubiquitin specific peptidase 25 Names ubiquitin carboxyl-terminal hydrolase 25
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Usp25	NP_038946.2
Rattus norvegicus (Norway rat)	Usp25	NP_001100584.1
Homo sapiens (human)	USP25	NP_001269970.1
	USP25	XP_001107973.2
Canis lupus familiaris (dog)	USP25	XP_535562.3
Xenopus tropicalis (tropical clawed frog)	usp25	NP_001039152.1
Bos taurus (cattle)	USP25	XP_005201215.1
Gallus gallus (chicken)	USP25	XP_004938410.1
Pan troglodytes (chimpanzee)	USP25	XP_531393.2
Danio rerio (zebrafish)	usp25	NP_001001886.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

USP25 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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