USP25 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001283041.1	NP_001269970.1	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25m
NM_013396.4	NP_037528.3	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
XM_017028323.1	XP_016883812.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_017028324.1	XP_016883813.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_006723992.3	XP_006724055.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
XM_024452064.1	XP_024307832.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
XM_011529535.2	XP_011527837.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
NM_001352561.1	NP_001339490.1	ubiquitin carboxyl-terminal hydrolase 25 isoform 5
XM_017028329.1	XP_016883818.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X10
NM_001283042.3	NP_001269971.1	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25b
NM_001352561.2	NP_001339490.1	ubiquitin carboxyl-terminal hydrolase 25 isoform 5
NM_013396.6	NP_037528.3	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
XM_017028327.1	XP_016883816.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
XM_005260949.4	XP_005261006.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
NM_001352560.1	NP_001339489.1	ubiquitin carboxyl-terminal hydrolase 25 isoform 4
NM_013396.5	NP_037528.3	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
XM_011529531.2	XP_011527833.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
XM_017028328.1	XP_016883817.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X9
NM_001352560.2	NP_001339489.1	ubiquitin carboxyl-terminal hydrolase 25 isoform 4
XM_024452063.1	XP_024307831.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_017028323.2	XP_016883812.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
NM_001283042.1	NP_001269971.1	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25b
XM_011529529.1	XP_011527831.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_017028322.1	XP_016883811.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
XM_017028325.1	XP_016883814.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
NM_001283042.2	NP_001269971.1	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25b
XM_017028321.1	XP_016883810.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
XM_011529533.2	XP_011527835.1	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
NM_001283041.2	NP_001269970.1	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25m

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu67365	XM_011529535.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu53233	XM_011529533.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53233	XM_006723992.3 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53234	XM_005260949.4 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53234	XM_017028327.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu67361	XM_011529529.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu67362	XM_017028322.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu67362	XM_017028323.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu67362	XM_024452063.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu67363	XM_024452064.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu67363	XM_011529531.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu93249	NM_001352560.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu93252	NM_001352561.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu93249	XM_017028324.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu93250	XM_017028325.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu93251	XM_017028328.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu93252	XM_017028329.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu93248	XM_017028321.1 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu11796	NM_001283042.3 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu12464	NM_001283041.2 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu12469	NM_013396.6 Latest version!	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
View Old Accession Versions >>
OHu93252	NM_001352561.1	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu12469	NM_013396.4	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu12469	NM_013396.5	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu93249	NM_001352560.1	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu67362	XM_017028323.1	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu11796	NM_001283042.1	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu11796	NM_001283042.2	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu12464	NM_001283041.1	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

USP25 ( XM_011529535.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527837.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu67365
Clone ID Related Accession (Same CDS sequence)	XM_011529535.2
Accession Version	XM_011529535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGGTTGTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527837.1
CDS	448..2151
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRGCF

Target ORF information:

RefSeq Version	XM_011529535.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529535.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGGTTGTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_006723992.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724055.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu53233
Clone ID Related Accession (Same CDS sequence)	XM_006723992.3 , XM_011529533.2
Accession Version	XM_006723992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATT CCTGGCTGTG GGAGTACTTG AAGGTAAAAA AATTTGAAAA CACTGAAATG 
TTTGGTCAGT ACCCACTTCA GGTCAATGGG TTCAAAGATC TGCATGAGTG CCTAGAAGCT 
GCAATGATTG AAGGAGAAAT TGAGTCTTTA CATTCAGAGA ATTCAGGAAA ATCAGGCCAA 
GAGCATTGGT TTACTGAATT ACCACCTGTG TTAACATTTG AATTGTCAAG ATTTGAATTT 
AATCAGGCAT TGGGAAGACC AGAAAAAATT CACAACAAAT TAGAATTTCC CCAAGTTTTA 
TATTTGGACA GATACATGCA CAGAAACAGA GAAATAACAA GAATTAAGAG GGAAGAGATC 
AAGAGACTGA AAGATTACCT CACGGTATTA CAACAAAGGC TAGAAAGATA TTTAAGCTAT 
GGTTCCGGTC CCAAACGATT CCCCTTGGTA GATGTTCTTC AGTATGCATT GGAATTTGCC 
TCAAGTAAAC CTGTTTGCAC TTCTCCTGTT GACGATATTG ACGCTAGTTC CCCACCTAGT 
GGTTCCATAC CATCACAGAC ATTACCAAGC ACAACAGAAC AACAGGGAGC CCTATCTTCA 
GAACTGCCAA GCACATCACC TTCATCAGTT GCTGCCATTT CATCGAGATC AGTAATACAC 
AAACCATTTA CTCAGTCCCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATAACGGAGG AAGAACTTTC TGTGCTGGAA AGTTGTTTAC ATCGCTGGAG GACAGAAATA 
GAAAATGACA CCAGAGATTT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAATTA 
ATGTACTCTG ACAAATCTAT GATACAAGTT CCTTATCGAT TACATGCCGT TTTAGTTCAC 
GAAGGCCAAG CTAATGCTGG GCACTACTGG GCATATATTT TTGATCATCG TGAAAGCAGA 
TGGATGAAGT ACAATGATAT TGCTGTGACA AAATCATCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTTGGTG GTTATAGAAA TGCCAGTGCA TACTGTTTAA TGTACATAAA TGATAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAAACTGGGC AGCCCCTTGT TGGTATAGAA 
ACATTACCAC CGGATTTGAG AGATTTTGTT GAGGAAGACA ACCAACGATT TGAAAAAGAA 
CTAGAAGAAT GGGATGCACA ACTTGCCCAG AAAGCTTTGC AGGAAAAGCT TTTAGCGTCT 
CAGAAATTGA GAGAGTCAGA GACTTCTGTG ACAACAGCAC AAGCAGCAGG AGACCCAGAA 
TATCTAGAGC AGCCATCAAG AAGTGATTTC TCAAAGCACT TGAAAGAAGA AACTATTCAA 
ATAATTACCA AGGCATCACA TGAGCATGAA GATAAAAGTC CTGAAACAGT TTTGCAGTCG 
AAACCTGAAA ATACTACAAG CCAACCACTT TCTAATCAGC GAGTTGTAGA GGTGGCGATC 
CCTCATGTAG GGAAATTTAT GATTGAATCA AAGGAGGGGG GGTATGATGA CGAGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCGATAGGTA AATCCAGGAG TGTATATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATGC AAGGTTGGTT 
AAGTTGGCCC AAGAAGACAC CCCACCAGAA ACCGATTATC GTTTACATCA TGTAGTGGTC 
TACTTTATCC AGAACCAGGC ACCAAAGAAA ATTATTGAGA AAACATTACT AGAACAATTT 
GGAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAACTGGAAA TGATAAAACC TGAAGAAGTA AACTTGGAGG AATATGAGGA GTGGCATCAG 
GATTATAGGA AATTCAGGGA AACAACTATG TATCTCATAA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTGTTC CTCATCTGTG CTTATCAGAA TAACAAAGAA 
CTCTTGTCTA AAGGCTTATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTGC TAAAATTAAA TGAGCAAGCC GCAGAACTCT TCGAATCTGG AGAGGATCGA 
GAAGTAAACA ATGGTTTGAT TATCATGAAT GAGTTTATTG TCCCATTTTT GCCATTATTA 
CTGGTGGATG AAATGGAAGA AAAGGATATA CTAGCTGTAG AAGATATGAG AAATCGATGG 
TGTTCCTACC TTGGTCAAGA AATGGAACCA CACCTCCAAG AAAAGCTGAC AGATTTTTTG 
CCAAAACTGC TTGATTGTTC TATGGAGATT AAAAGTTTCC ATGAGCCACC GAAGTTACCT 
TCATATTCCA CGCATGAACT CTGTGAGCGA TTTGCCCGAA TCATGTTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724055.1
CDS	119..2599
Translation	MADSWLWEYLKVKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_006723992.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723992.3

ORF Insert Sequence:

ATGGCAGATT CCTGGCTGTG GGAGTACTTG AAGGTAAAAA AATTTGAAAA CACTGAAATG 
TTTGGTCAGT ACCCACTTCA GGTCAATGGG TTCAAAGATC TGCATGAGTG CCTAGAAGCT 
GCAATGATTG AAGGAGAAAT TGAGTCTTTA CATTCAGAGA ATTCAGGAAA ATCAGGCCAA 
GAGCATTGGT TTACTGAATT ACCACCTGTG TTAACATTTG AATTGTCAAG ATTTGAATTT 
AATCAGGCAT TGGGAAGACC AGAAAAAATT CACAACAAAT TAGAATTTCC CCAAGTTTTA 
TATTTGGACA GATACATGCA CAGAAACAGA GAAATAACAA GAATTAAGAG GGAAGAGATC 
AAGAGACTGA AAGATTACCT CACGGTATTA CAACAAAGGC TAGAAAGATA TTTAAGCTAT 
GGTTCCGGTC CCAAACGATT CCCCTTGGTA GATGTTCTTC AGTATGCATT GGAATTTGCC 
TCAAGTAAAC CTGTTTGCAC TTCTCCTGTT GACGATATTG ACGCTAGTTC CCCACCTAGT 
GGTTCCATAC CATCACAGAC ATTACCAAGC ACAACAGAAC AACAGGGAGC CCTATCTTCA 
GAACTGCCAA GCACATCACC TTCATCAGTT GCTGCCATTT CATCGAGATC AGTAATACAC 
AAACCATTTA CTCAGTCCCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATAACGGAGG AAGAACTTTC TGTGCTGGAA AGTTGTTTAC ATCGCTGGAG GACAGAAATA 
GAAAATGACA CCAGAGATTT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAATTA 
ATGTACTCTG ACAAATCTAT GATACAAGTT CCTTATCGAT TACATGCCGT TTTAGTTCAC 
GAAGGCCAAG CTAATGCTGG GCACTACTGG GCATATATTT TTGATCATCG TGAAAGCAGA 
TGGATGAAGT ACAATGATAT TGCTGTGACA AAATCATCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTTGGTG GTTATAGAAA TGCCAGTGCA TACTGTTTAA TGTACATAAA TGATAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAAACTGGGC AGCCCCTTGT TGGTATAGAA 
ACATTACCAC CGGATTTGAG AGATTTTGTT GAGGAAGACA ACCAACGATT TGAAAAAGAA 
CTAGAAGAAT GGGATGCACA ACTTGCCCAG AAAGCTTTGC AGGAAAAGCT TTTAGCGTCT 
CAGAAATTGA GAGAGTCAGA GACTTCTGTG ACAACAGCAC AAGCAGCAGG AGACCCAGAA 
TATCTAGAGC AGCCATCAAG AAGTGATTTC TCAAAGCACT TGAAAGAAGA AACTATTCAA 
ATAATTACCA AGGCATCACA TGAGCATGAA GATAAAAGTC CTGAAACAGT TTTGCAGTCG 
AAACCTGAAA ATACTACAAG CCAACCACTT TCTAATCAGC GAGTTGTAGA GGTGGCGATC 
CCTCATGTAG GGAAATTTAT GATTGAATCA AAGGAGGGGG GGTATGATGA CGAGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCGATAGGTA AATCCAGGAG TGTATATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATGC AAGGTTGGTT 
AAGTTGGCCC AAGAAGACAC CCCACCAGAA ACCGATTATC GTTTACATCA TGTAGTGGTC 
TACTTTATCC AGAACCAGGC ACCAAAGAAA ATTATTGAGA AAACATTACT AGAACAATTT 
GGAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAACTGGAAA TGATAAAACC TGAAGAAGTA AACTTGGAGG AATATGAGGA GTGGCATCAG 
GATTATAGGA AATTCAGGGA AACAACTATG TATCTCATAA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTGTTC CTCATCTGTG CTTATCAGAA TAACAAAGAA 
CTCTTGTCTA AAGGCTTATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTGC TAAAATTAAA TGAGCAAGCC GCAGAACTCT TCGAATCTGG AGAGGATCGA 
GAAGTAAACA ATGGTTTGAT TATCATGAAT GAGTTTATTG TCCCATTTTT GCCATTATTA 
CTGGTGGATG AAATGGAAGA AAAGGATATA CTAGCTGTAG AAGATATGAG AAATCGATGG 
TGTTCCTACC TTGGTCAAGA AATGGAACCA CACCTCCAAG AAAAGCTGAC AGATTTTTTG 
CCAAAACTGC TTGATTGTTC TATGGAGATT AAAAGTTTCC ATGAGCCACC GAAGTTACCT 
TCATATTCCA CGCATGAACT CTGTGAGCGA TTTGCCCGAA TCATGTTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_011529533.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527835.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu53233
Clone ID Related Accession (Same CDS sequence)	XM_006723992.3 , XM_011529533.2
Accession Version	XM_011529533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATT CCTGGCTGTG GGAGTACTTG AAGGTAAAAA AATTTGAAAA CACTGAAATG 
TTTGGTCAGT ACCCACTTCA GGTCAATGGG TTCAAAGATC TGCATGAGTG CCTAGAAGCT 
GCAATGATTG AAGGAGAAAT TGAGTCTTTA CATTCAGAGA ATTCAGGAAA ATCAGGCCAA 
GAGCATTGGT TTACTGAATT ACCACCTGTG TTAACATTTG AATTGTCAAG ATTTGAATTT 
AATCAGGCAT TGGGAAGACC AGAAAAAATT CACAACAAAT TAGAATTTCC CCAAGTTTTA 
TATTTGGACA GATACATGCA CAGAAACAGA GAAATAACAA GAATTAAGAG GGAAGAGATC 
AAGAGACTGA AAGATTACCT CACGGTATTA CAACAAAGGC TAGAAAGATA TTTAAGCTAT 
GGTTCCGGTC CCAAACGATT CCCCTTGGTA GATGTTCTTC AGTATGCATT GGAATTTGCC 
TCAAGTAAAC CTGTTTGCAC TTCTCCTGTT GACGATATTG ACGCTAGTTC CCCACCTAGT 
GGTTCCATAC CATCACAGAC ATTACCAAGC ACAACAGAAC AACAGGGAGC CCTATCTTCA 
GAACTGCCAA GCACATCACC TTCATCAGTT GCTGCCATTT CATCGAGATC AGTAATACAC 
AAACCATTTA CTCAGTCCCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATAACGGAGG AAGAACTTTC TGTGCTGGAA AGTTGTTTAC ATCGCTGGAG GACAGAAATA 
GAAAATGACA CCAGAGATTT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAATTA 
ATGTACTCTG ACAAATCTAT GATACAAGTT CCTTATCGAT TACATGCCGT TTTAGTTCAC 
GAAGGCCAAG CTAATGCTGG GCACTACTGG GCATATATTT TTGATCATCG TGAAAGCAGA 
TGGATGAAGT ACAATGATAT TGCTGTGACA AAATCATCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTTGGTG GTTATAGAAA TGCCAGTGCA TACTGTTTAA TGTACATAAA TGATAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAAACTGGGC AGCCCCTTGT TGGTATAGAA 
ACATTACCAC CGGATTTGAG AGATTTTGTT GAGGAAGACA ACCAACGATT TGAAAAAGAA 
CTAGAAGAAT GGGATGCACA ACTTGCCCAG AAAGCTTTGC AGGAAAAGCT TTTAGCGTCT 
CAGAAATTGA GAGAGTCAGA GACTTCTGTG ACAACAGCAC AAGCAGCAGG AGACCCAGAA 
TATCTAGAGC AGCCATCAAG AAGTGATTTC TCAAAGCACT TGAAAGAAGA AACTATTCAA 
ATAATTACCA AGGCATCACA TGAGCATGAA GATAAAAGTC CTGAAACAGT TTTGCAGTCG 
AAACCTGAAA ATACTACAAG CCAACCACTT TCTAATCAGC GAGTTGTAGA GGTGGCGATC 
CCTCATGTAG GGAAATTTAT GATTGAATCA AAGGAGGGGG GGTATGATGA CGAGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCGATAGGTA AATCCAGGAG TGTATATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATGC AAGGTTGGTT 
AAGTTGGCCC AAGAAGACAC CCCACCAGAA ACCGATTATC GTTTACATCA TGTAGTGGTC 
TACTTTATCC AGAACCAGGC ACCAAAGAAA ATTATTGAGA AAACATTACT AGAACAATTT 
GGAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAACTGGAAA TGATAAAACC TGAAGAAGTA AACTTGGAGG AATATGAGGA GTGGCATCAG 
GATTATAGGA AATTCAGGGA AACAACTATG TATCTCATAA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTGTTC CTCATCTGTG CTTATCAGAA TAACAAAGAA 
CTCTTGTCTA AAGGCTTATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTGC TAAAATTAAA TGAGCAAGCC GCAGAACTCT TCGAATCTGG AGAGGATCGA 
GAAGTAAACA ATGGTTTGAT TATCATGAAT GAGTTTATTG TCCCATTTTT GCCATTATTA 
CTGGTGGATG AAATGGAAGA AAAGGATATA CTAGCTGTAG AAGATATGAG AAATCGATGG 
TGTTCCTACC TTGGTCAAGA AATGGAACCA CACCTCCAAG AAAAGCTGAC AGATTTTTTG 
CCAAAACTGC TTGATTGTTC TATGGAGATT AAAAGTTTCC ATGAGCCACC GAAGTTACCT 
TCATATTCCA CGCATGAACT CTGTGAGCGA TTTGCCCGAA TCATGTTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527835.1
CDS	104..2584
Translation	MADSWLWEYLKVKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_011529533.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529533.2

ORF Insert Sequence:

ATGGCAGATT CCTGGCTGTG GGAGTACTTG AAGGTAAAAA AATTTGAAAA CACTGAAATG 
TTTGGTCAGT ACCCACTTCA GGTCAATGGG TTCAAAGATC TGCATGAGTG CCTAGAAGCT 
GCAATGATTG AAGGAGAAAT TGAGTCTTTA CATTCAGAGA ATTCAGGAAA ATCAGGCCAA 
GAGCATTGGT TTACTGAATT ACCACCTGTG TTAACATTTG AATTGTCAAG ATTTGAATTT 
AATCAGGCAT TGGGAAGACC AGAAAAAATT CACAACAAAT TAGAATTTCC CCAAGTTTTA 
TATTTGGACA GATACATGCA CAGAAACAGA GAAATAACAA GAATTAAGAG GGAAGAGATC 
AAGAGACTGA AAGATTACCT CACGGTATTA CAACAAAGGC TAGAAAGATA TTTAAGCTAT 
GGTTCCGGTC CCAAACGATT CCCCTTGGTA GATGTTCTTC AGTATGCATT GGAATTTGCC 
TCAAGTAAAC CTGTTTGCAC TTCTCCTGTT GACGATATTG ACGCTAGTTC CCCACCTAGT 
GGTTCCATAC CATCACAGAC ATTACCAAGC ACAACAGAAC AACAGGGAGC CCTATCTTCA 
GAACTGCCAA GCACATCACC TTCATCAGTT GCTGCCATTT CATCGAGATC AGTAATACAC 
AAACCATTTA CTCAGTCCCG GATACCTCCA GATTTGCCCA TGCATCCGGC ACCAAGGCAC 
ATAACGGAGG AAGAACTTTC TGTGCTGGAA AGTTGTTTAC ATCGCTGGAG GACAGAAATA 
GAAAATGACA CCAGAGATTT GCAGGAAAGC ATATCCAGAA TCCATCGAAC AATTGAATTA 
ATGTACTCTG ACAAATCTAT GATACAAGTT CCTTATCGAT TACATGCCGT TTTAGTTCAC 
GAAGGCCAAG CTAATGCTGG GCACTACTGG GCATATATTT TTGATCATCG TGAAAGCAGA 
TGGATGAAGT ACAATGATAT TGCTGTGACA AAATCATCAT GGGAAGAGCT AGTGAGGGAC 
TCTTTTGGTG GTTATAGAAA TGCCAGTGCA TACTGTTTAA TGTACATAAA TGATAAGGCA 
CAGTTCCTAA TACAAGAGGA GTTTAATAAA GAAACTGGGC AGCCCCTTGT TGGTATAGAA 
ACATTACCAC CGGATTTGAG AGATTTTGTT GAGGAAGACA ACCAACGATT TGAAAAAGAA 
CTAGAAGAAT GGGATGCACA ACTTGCCCAG AAAGCTTTGC AGGAAAAGCT TTTAGCGTCT 
CAGAAATTGA GAGAGTCAGA GACTTCTGTG ACAACAGCAC AAGCAGCAGG AGACCCAGAA 
TATCTAGAGC AGCCATCAAG AAGTGATTTC TCAAAGCACT TGAAAGAAGA AACTATTCAA 
ATAATTACCA AGGCATCACA TGAGCATGAA GATAAAAGTC CTGAAACAGT TTTGCAGTCG 
AAACCTGAAA ATACTACAAG CCAACCACTT TCTAATCAGC GAGTTGTAGA GGTGGCGATC 
CCTCATGTAG GGAAATTTAT GATTGAATCA AAGGAGGGGG GGTATGATGA CGAGATCATG 
ATGACACCGA ACATGCAAGG TATTATCATG GCGATAGGTA AATCCAGGAG TGTATATGAC 
AGGTGTGGCC CTGAAGCAGG GTTCTTTAAG GCAATTAAGT TGGAATATGC AAGGTTGGTT 
AAGTTGGCCC AAGAAGACAC CCCACCAGAA ACCGATTATC GTTTACATCA TGTAGTGGTC 
TACTTTATCC AGAACCAGGC ACCAAAGAAA ATTATTGAGA AAACATTACT AGAACAATTT 
GGAGATAGAA ATTTGAGTTT TGATGAAAGG TGTCACAACA TAATGAAAGT TGCTCAAGCC 
AAACTGGAAA TGATAAAACC TGAAGAAGTA AACTTGGAGG AATATGAGGA GTGGCATCAG 
GATTATAGGA AATTCAGGGA AACAACTATG TATCTCATAA TTGGGCTAGA AAATTTTCAA 
AGAGAAAGTT ATATAGATTC CTTGCTGTTC CTCATCTGTG CTTATCAGAA TAACAAAGAA 
CTCTTGTCTA AAGGCTTATA CAGAGGACAT GATGAAGAAT TGATATCACA TTATAGAAGA 
GAATGTTTGC TAAAATTAAA TGAGCAAGCC GCAGAACTCT TCGAATCTGG AGAGGATCGA 
GAAGTAAACA ATGGTTTGAT TATCATGAAT GAGTTTATTG TCCCATTTTT GCCATTATTA 
CTGGTGGATG AAATGGAAGA AAAGGATATA CTAGCTGTAG AAGATATGAG AAATCGATGG 
TGTTCCTACC TTGGTCAAGA AATGGAACCA CACCTCCAAG AAAAGCTGAC AGATTTTTTG 
CCAAAACTGC TTGATTGTTC TATGGAGATT AAAAGTTTCC ATGAGCCACC GAAGTTACCT 
TCATATTCCA CGCATGAACT CTGTGAGCGA TTTGCCCGAA TCATGTTGTC CCTCAGTCGA 
ACTCCTGCTG ATGGAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028327.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883816.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu53234
Clone ID Related Accession (Same CDS sequence)	XM_017028327.1 , XM_005260949.4
Accession Version	XM_017028327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883816.1
CDS	594..2759
Translation	MHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028327.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028327.1

ORF Insert Sequence:

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_005260949.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261006.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu53234
Clone ID Related Accession (Same CDS sequence)	XM_017028327.1 , XM_005260949.4
Accession Version	XM_005260949.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260949.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261006.1
CDS	131..2296
Translation	MHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_005260949.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260949.4

ORF Insert Sequence:

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_011529529.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527831.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu67361
Clone ID Related Accession (Same CDS sequence)	XM_011529529.1
Accession Version	XM_011529529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGAAA 
CCTGAAAATA CTACAAGCCA ACCACTTTCT AATCAGCGAG TTGTAGAGGT GGCGATCCCT 
CATGTAGGGA AATTTATGAT TGAATCAAAG GAGGGGGGGT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AATATGCAAG GTTGGTTAAG 
TTGGCCCAAG AAGACACCCC ACCAGAAACC GATTATCGTT TACATCATGT AGTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATT ATTGAGAAAA CATTACTAGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TAAAACCTGA AGAAGTAAAC TTGGAGGAAT ATGAGTGCAG GCTGTATGAA 
AATTCCAGCA AGATGTTTTA TGGAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527831.1
CDS	448..3297
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYECRLYENSSKMFYGA

Target ORF information:

RefSeq Version	XM_011529529.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529529.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGAAA 
CCTGAAAATA CTACAAGCCA ACCACTTTCT AATCAGCGAG TTGTAGAGGT GGCGATCCCT 
CATGTAGGGA AATTTATGAT TGAATCAAAG GAGGGGGGGT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AATATGCAAG GTTGGTTAAG 
TTGGCCCAAG AAGACACCCC ACCAGAAACC GATTATCGTT TACATCATGT AGTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATT ATTGAGAAAA CATTACTAGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TAAAACCTGA AGAAGTAAAC TTGGAGGAAT ATGAGTGCAG GCTGTATGAA 
AATTCCAGCA AGATGTTTTA TGGAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028323.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883812.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu67362
Clone ID Related Accession (Same CDS sequence)	XM_017028323.1 , XM_024452063.1 , XM_017028323.2 , XM_017028322.1
Accession Version	XM_017028323.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883812.1
CDS	432..3146
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028323.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028323.1

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_024452063.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307831.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu67362
Clone ID Related Accession (Same CDS sequence)	XM_017028323.1 , XM_024452063.1 , XM_017028323.2 , XM_017028322.1
Accession Version	XM_024452063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307831.1
CDS	885..3599
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_024452063.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452063.1

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028323.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883812.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu67362
Clone ID Related Accession (Same CDS sequence)	XM_017028323.1 , XM_024452063.1 , XM_017028323.2 , XM_017028322.1
Accession Version	XM_017028323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883812.1
CDS	430..3144
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028323.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028323.2

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028322.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883811.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

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CloneID	OHu67362
Clone ID Related Accession (Same CDS sequence)	XM_017028323.1 , XM_024452063.1 , XM_017028323.2 , XM_017028322.1
Accession Version	XM_017028322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883811.1
CDS	987..3701
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028322.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028322.1

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGAAACCT 
GAAAATACTA CAAGCCAACC ACTTTCTAAT CAGCGAGTTG TAGAGGTGGC GATCCCTCAT 
GTAGGGAAAT TTATGATTGA ATCAAAGGAG GGGGGGTATG ATGACGAGAT CATGATGACA 
CCGAACATGC AAGGTATTAT CATGGCGATA GGTAAATCCA GGAGTGTATA TGACAGGTGT 
GGCCCTGAAG CAGGGTTCTT TAAGGCAATT AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG 
GCCCAAGAAG ACACCCCACC AGAAACCGAT TATCGTTTAC ATCATGTAGT GGTCTACTTT 
ATCCAGAACC AGGCACCAAA GAAAATTATT GAGAAAACAT TACTAGAACA ATTTGGAGAT 
AGAAATTTGA GTTTTGATGA AAGGTGTCAC AACATAATGA AAGTTGCTCA AGCCAAACTG 
GAAATGATAA AACCTGAAGA AGTAAACTTG GAGGAATATG AGGAGTGGCA TCAGGATTAT 
AGGAAATTCA GGGAAACAAC TATGTATCTC ATAATTGGGC TAGAAAATTT TCAAAGAGAA 
AGTTATATAG ATTCCTTGCT GTTCCTCATC TGTGCTTATC AGAATAACAA AGAACTCTTG 
TCTAAAGGCT TATACAGAGG ACATGATGAA GAATTGATAT CACATTATAG AAGAGAATGT 
TTGCTAAAAT TAAATGAGCA AGCCGCAGAA CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA 
AACAATGGTT TGATTATCAT GAATGAGTTT ATTGTCCCAT TTTTGCCATT ATTACTGGTG 
GATGAAATGG AAGAAAAGGA TATACTAGCT GTAGAAGATA TGAGAAATCG ATGGTGTTCC 
TACCTTGGTC AAGAAATGGA ACCACACCTC CAAGAAAAGC TGACAGATTT TTTGCCAAAA 
CTGCTTGATT GTTCTATGGA GATTAAAAGT TTCCATGAGC CACCGAAGTT ACCTTCATAT 
TCCACGCATG AACTCTGTGA GCGATTTGCC CGAATCATGT TGTCCCTCAG TCGAACTCCT 
GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_024452064.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307832.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu67363
Clone ID Related Accession (Same CDS sequence)	XM_024452064.1 , XM_011529531.2
Accession Version	XM_024452064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGATCC ATCAAGAGCA GTTGAAATTC TTAAGGATGC TTTCAAATCA AATGACTCAC 
AGCAGGGATG AAGAGAAGCC AAAGAACCCC ATGGTAGAGT TGTTCTATGG CAGATTCCTG 
GCTGTGGGAG TACTTGAAGG TAAAAAATTT GAAAACACTG AAATGTTTGG TCAGTACCCA 
CTTCAGGTCA ATGGGTTCAA AGATCTGCAT GAGTGCCTAG AAGCTGCAAT GATTGAAGGA 
GAAATTGAGT CTTTACATTC AGAGAATTCA GGAAAATCAG GCCAAGAGCA TTGGTTTACT 
GAATTACCAC CTGTGTTAAC ATTTGAATTG TCAAGATTTG AATTTAATCA GGCATTGGGA 
AGACCAGAAA AAATTCACAA CAAATTAGAA TTTCCCCAAG TTTTATATTT GGACAGATAC 
ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307832.1
CDS	627..3212
Translation	MLIHQEQLKFLRMLSNQMTHSRDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_024452064.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452064.1

ORF Insert Sequence:

ATGTTGATCC ATCAAGAGCA GTTGAAATTC TTAAGGATGC TTTCAAATCA AATGACTCAC 
AGCAGGGATG AAGAGAAGCC AAAGAACCCC ATGGTAGAGT TGTTCTATGG CAGATTCCTG 
GCTGTGGGAG TACTTGAAGG TAAAAAATTT GAAAACACTG AAATGTTTGG TCAGTACCCA 
CTTCAGGTCA ATGGGTTCAA AGATCTGCAT GAGTGCCTAG AAGCTGCAAT GATTGAAGGA 
GAAATTGAGT CTTTACATTC AGAGAATTCA GGAAAATCAG GCCAAGAGCA TTGGTTTACT 
GAATTACCAC CTGTGTTAAC ATTTGAATTG TCAAGATTTG AATTTAATCA GGCATTGGGA 
AGACCAGAAA AAATTCACAA CAAATTAGAA TTTCCCCAAG TTTTATATTT GGACAGATAC 
ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_011529531.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527833.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

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CloneID	OHu67363
Clone ID Related Accession (Same CDS sequence)	XM_024452064.1 , XM_011529531.2
Accession Version	XM_011529531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGATCC ATCAAGAGCA GTTGAAATTC TTAAGGATGC TTTCAAATCA AATGACTCAC 
AGCAGGGATG AAGAGAAGCC AAAGAACCCC ATGGTAGAGT TGTTCTATGG CAGATTCCTG 
GCTGTGGGAG TACTTGAAGG TAAAAAATTT GAAAACACTG AAATGTTTGG TCAGTACCCA 
CTTCAGGTCA ATGGGTTCAA AGATCTGCAT GAGTGCCTAG AAGCTGCAAT GATTGAAGGA 
GAAATTGAGT CTTTACATTC AGAGAATTCA GGAAAATCAG GCCAAGAGCA TTGGTTTACT 
GAATTACCAC CTGTGTTAAC ATTTGAATTG TCAAGATTTG AATTTAATCA GGCATTGGGA 
AGACCAGAAA AAATTCACAA CAAATTAGAA TTTCCCCAAG TTTTATATTT GGACAGATAC 
ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527833.1
CDS	937..3522
Translation	MLIHQEQLKFLRMLSNQMTHSRDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_011529531.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529531.2

ORF Insert Sequence:

ATGTTGATCC ATCAAGAGCA GTTGAAATTC TTAAGGATGC TTTCAAATCA AATGACTCAC 
AGCAGGGATG AAGAGAAGCC AAAGAACCCC ATGGTAGAGT TGTTCTATGG CAGATTCCTG 
GCTGTGGGAG TACTTGAAGG TAAAAAATTT GAAAACACTG AAATGTTTGG TCAGTACCCA 
CTTCAGGTCA ATGGGTTCAA AGATCTGCAT GAGTGCCTAG AAGCTGCAAT GATTGAAGGA 
GAAATTGAGT CTTTACATTC AGAGAATTCA GGAAAATCAG GCCAAGAGCA TTGGTTTACT 
GAATTACCAC CTGTGTTAAC ATTTGAATTG TCAAGATTTG AATTTAATCA GGCATTGGGA 
AGACCAGAAA AAATTCACAA CAAATTAGAA TTTCCCCAAG TTTTATATTT GGACAGATAC 
ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGAAACC TGAAAATACT 
ACAAGCCAAC CACTTTCTAA TCAGCGAGTT GTAGAGGTGG CGATCCCTCA TGTAGGGAAA 
TTTATGATTG AATCAAAGGA GGGGGGGTAT GATGACGAGA TCATGATGAC ACCGAACATG 
CAAGGTATTA TCATGGCGAT AGGTAAATCC AGGAGTGTAT ATGACAGGTG TGGCCCTGAA 
GCAGGGTTCT TTAAGGCAAT TAAGTTGGAA TATGCAAGGT TGGTTAAGTT GGCCCAAGAA 
GACACCCCAC CAGAAACCGA TTATCGTTTA CATCATGTAG TGGTCTACTT TATCCAGAAC 
CAGGCACCAA AGAAAATTAT TGAGAAAACA TTACTAGAAC AATTTGGAGA TAGAAATTTG 
AGTTTTGATG AAAGGTGTCA CAACATAATG AAAGTTGCTC AAGCCAAACT GGAAATGATA 
AAACCTGAAG AAGTAAACTT GGAGGAATAT GAGGAGTGGC ATCAGGATTA TAGGAAATTC 
AGGGAAACAA CTATGTATCT CATAATTGGG CTAGAAAATT TTCAAAGAGA AAGTTATATA 
GATTCCTTGC TGTTCCTCAT CTGTGCTTAT CAGAATAACA AAGAACTCTT GTCTAAAGGC 
TTATACAGAG GACATGATGA AGAATTGATA TCACATTATA GAAGAGAATG TTTGCTAAAA 
TTAAATGAGC AAGCCGCAGA ACTCTTCGAA TCTGGAGAGG ATCGAGAAGT AAACAATGGT 
TTGATTATCA TGAATGAGTT TATTGTCCCA TTTTTGCCAT TATTACTGGT GGATGAAATG 
GAAGAAAAGG ATATACTAGC TGTAGAAGAT ATGAGAAATC GATGGTGTTC CTACCTTGGT 
CAAGAAATGG AACCACACCT CCAAGAAAAG CTGACAGATT TTTTGCCAAA ACTGCTTGAT 
TGTTCTATGG AGATTAAAAG TTTCCATGAG CCACCGAAGT TACCTTCATA TTCCACGCAT 
GAACTCTGTG AGCGATTTGC CCGAATCATG TTGTCCCTCA GTCGAACTCC TGCTGATGGA 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001352560.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339489.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 4, mRNA.

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CloneID	OHu93249
Clone ID Related Accession (Same CDS sequence)	XM_017028324.1 , NM_001352560.1 , NM_001352560.2
Accession Version	NM_001352560.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, AJ010598.1 and BM149594.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.213485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG GCCCAAGAAG ACACCCCACC AGAAACCGAT 
TATCGTTTAC ATCATGTAGT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATTATT 
GAGAAAACAT TACTAGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAACTG GAAATGATAA AACCTGAAGA AGTAAACTTG 
GAGGAATATG AGGAGTGGCA TCAGGATTAT AGGAAATTCA GGGAAACAAC TATGTATCTC 
ATAATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT GTTCCTCATC 
TGTGCTTATC AGAATAACAA AGAACTCTTG TCTAAAGGCT TATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTGCTAAAAT TAAATGAGCA AGCCGCAGAA 
CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA AACAATGGTT TGATTATCAT GAATGAGTTT 
ATTGTCCCAT TTTTGCCATT ATTACTGGTG GATGAAATGG AAGAAAAGGA TATACTAGCT 
GTAGAAGATA TGAGAAATCG ATGGTGTTCC TACCTTGGTC AAGAAATGGA ACCACACCTC 
CAAGAAAAGC TGACAGATTT TTTGCCAAAA CTGCTTGATT GTTCTATGGA GATTAAAAGT 
TTCCATGAGC CACCGAAGTT ACCTTCATAT TCCACGCATG AACTCTGTGA GCGATTTGCC 
CGAATCATGT TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339489.1
CDS	885..3389
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001352560.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352560.1

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG GCCCAAGAAG ACACCCCACC AGAAACCGAT 
TATCGTTTAC ATCATGTAGT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATTATT 
GAGAAAACAT TACTAGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAACTG GAAATGATAA AACCTGAAGA AGTAAACTTG 
GAGGAATATG AGGAGTGGCA TCAGGATTAT AGGAAATTCA GGGAAACAAC TATGTATCTC 
ATAATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT GTTCCTCATC 
TGTGCTTATC AGAATAACAA AGAACTCTTG TCTAAAGGCT TATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTGCTAAAAT TAAATGAGCA AGCCGCAGAA 
CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA AACAATGGTT TGATTATCAT GAATGAGTTT 
ATTGTCCCAT TTTTGCCATT ATTACTGGTG GATGAAATGG AAGAAAAGGA TATACTAGCT 
GTAGAAGATA TGAGAAATCG ATGGTGTTCC TACCTTGGTC AAGAAATGGA ACCACACCTC 
CAAGAAAAGC TGACAGATTT TTTGCCAAAA CTGCTTGATT GTTCTATGGA GATTAAAAGT 
TTCCATGAGC CACCGAAGTT ACCTTCATAT TCCACGCATG AACTCTGTGA GCGATTTGCC 
CGAATCATGT TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001352560.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339489.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 4, mRNA.

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CloneID	OHu93249
Clone ID Related Accession (Same CDS sequence)	XM_017028324.1 , NM_001352560.1 , NM_001352560.2
Accession Version	NM_001352560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, AJ010598.1 and BM149594.1. On Jun 2, 2019 this sequence version replaced NM_001352560.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.213485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG GCCCAAGAAG ACACCCCACC AGAAACCGAT 
TATCGTTTAC ATCATGTAGT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATTATT 
GAGAAAACAT TACTAGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAACTG GAAATGATAA AACCTGAAGA AGTAAACTTG 
GAGGAATATG AGGAGTGGCA TCAGGATTAT AGGAAATTCA GGGAAACAAC TATGTATCTC 
ATAATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT GTTCCTCATC 
TGTGCTTATC AGAATAACAA AGAACTCTTG TCTAAAGGCT TATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTGCTAAAAT TAAATGAGCA AGCCGCAGAA 
CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA AACAATGGTT TGATTATCAT GAATGAGTTT 
ATTGTCCCAT TTTTGCCATT ATTACTGGTG GATGAAATGG AAGAAAAGGA TATACTAGCT 
GTAGAAGATA TGAGAAATCG ATGGTGTTCC TACCTTGGTC AAGAAATGGA ACCACACCTC 
CAAGAAAAGC TGACAGATTT TTTGCCAAAA CTGCTTGATT GTTCTATGGA GATTAAAAGT 
TTCCATGAGC CACCGAAGTT ACCTTCATAT TCCACGCATG AACTCTGTGA GCGATTTGCC 
CGAATCATGT TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339489.1
CDS	1326..3830
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001352560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352560.2

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG GCCCAAGAAG ACACCCCACC AGAAACCGAT 
TATCGTTTAC ATCATGTAGT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATTATT 
GAGAAAACAT TACTAGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAACTG GAAATGATAA AACCTGAAGA AGTAAACTTG 
GAGGAATATG AGGAGTGGCA TCAGGATTAT AGGAAATTCA GGGAAACAAC TATGTATCTC 
ATAATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT GTTCCTCATC 
TGTGCTTATC AGAATAACAA AGAACTCTTG TCTAAAGGCT TATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTGCTAAAAT TAAATGAGCA AGCCGCAGAA 
CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA AACAATGGTT TGATTATCAT GAATGAGTTT 
ATTGTCCCAT TTTTGCCATT ATTACTGGTG GATGAAATGG AAGAAAAGGA TATACTAGCT 
GTAGAAGATA TGAGAAATCG ATGGTGTTCC TACCTTGGTC AAGAAATGGA ACCACACCTC 
CAAGAAAAGC TGACAGATTT TTTGCCAAAA CTGCTTGATT GTTCTATGGA GATTAAAAGT 
TTCCATGAGC CACCGAAGTT ACCTTCATAT TCCACGCATG AACTCTGTGA GCGATTTGCC 
CGAATCATGT TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001352561.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339490.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu93252
Clone ID Related Accession (Same CDS sequence)	NM_001352561.1 , XM_017028329.1 , NM_001352561.2
Accession Version	NM_001352561.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, AJ010598.1 and BM149594.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.173771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGGCAAT TAAGTTGGAA 
TATGCAAGGT TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA 
CATCATGTAG TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA 
TTACTAGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT 
GAGGAGTGGC ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT 
CAGAATAACA AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA 
TCTGGAGAGG ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA 
TTTTTGCCAT TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT 
ATGAGAAATC GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG 
CTGACAGATT TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG 
CCACCGAAGT TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG 
TTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339490.1
CDS	1673..3628
Translation	MHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001352561.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352561.1

ORF Insert Sequence:

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGGCAAT TAAGTTGGAA 
TATGCAAGGT TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA 
CATCATGTAG TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA 
TTACTAGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT 
GAGGAGTGGC ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT 
CAGAATAACA AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA 
TCTGGAGAGG ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA 
TTTTTGCCAT TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT 
ATGAGAAATC GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG 
CTGACAGATT TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG 
CCACCGAAGT TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG 
TTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001352561.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339490.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 5, mRNA.

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CloneID	OHu93252
Clone ID Related Accession (Same CDS sequence)	NM_001352561.1 , XM_017028329.1 , NM_001352561.2
Accession Version	NM_001352561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, AJ010598.1 and BM149594.1. On Jun 2, 2019 this sequence version replaced NM_001352561.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.173771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGGCAAT TAAGTTGGAA 
TATGCAAGGT TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA 
CATCATGTAG TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA 
TTACTAGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT 
GAGGAGTGGC ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT 
CAGAATAACA AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA 
TCTGGAGAGG ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA 
TTTTTGCCAT TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT 
ATGAGAAATC GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG 
CTGACAGATT TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG 
CCACCGAAGT TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG 
TTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339490.1
CDS	1625..3580
Translation	MHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001352561.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352561.2

ORF Insert Sequence:

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGGCAAT TAAGTTGGAA 
TATGCAAGGT TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA 
CATCATGTAG TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA 
TTACTAGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT 
GAGGAGTGGC ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT 
CAGAATAACA AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA 
TCTGGAGAGG ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA 
TTTTTGCCAT TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT 
ATGAGAAATC GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG 
CTGACAGATT TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG 
CCACCGAAGT TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG 
TTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028324.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883813.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

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CloneID	OHu93249
Clone ID Related Accession (Same CDS sequence)	XM_017028324.1 , NM_001352560.1 , NM_001352560.2
Accession Version	XM_017028324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG GCCCAAGAAG ACACCCCACC AGAAACCGAT 
TATCGTTTAC ATCATGTAGT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATTATT 
GAGAAAACAT TACTAGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAACTG GAAATGATAA AACCTGAAGA AGTAAACTTG 
GAGGAATATG AGGAGTGGCA TCAGGATTAT AGGAAATTCA GGGAAACAAC TATGTATCTC 
ATAATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT GTTCCTCATC 
TGTGCTTATC AGAATAACAA AGAACTCTTG TCTAAAGGCT TATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTGCTAAAAT TAAATGAGCA AGCCGCAGAA 
CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA AACAATGGTT TGATTATCAT GAATGAGTTT 
ATTGTCCCAT TTTTGCCATT ATTACTGGTG GATGAAATGG AAGAAAAGGA TATACTAGCT 
GTAGAAGATA TGAGAAATCG ATGGTGTTCC TACCTTGGTC AAGAAATGGA ACCACACCTC 
CAAGAAAAGC TGACAGATTT TTTGCCAAAA CTGCTTGATT GTTCTATGGA GATTAAAAGT 
TTCCATGAGC CACCGAAGTT ACCTTCATAT TCCACGCATG AACTCTGTGA GCGATTTGCC 
CGAATCATGT TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883813.1
CDS	885..3389
Translation	MRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028324.1

ORF Insert Sequence:

ATGCGTGAGC TGAGGTATCT ATTTGCACTT CTTGTTGGTA CCAAAAGGAA GTATGTTGAT 
CCATCAAGAG CAGTTGAAAT TCTTAAGGAT GCTTTCAAAT CAAATGACTC ACAGCAGCAA 
GATGTGAGTG AGTTTACACA CAAATTATTA GATTGGTTAG AAGATGCCTT CCAAATGAAA 
GCTGAAGAGG AGACGGATGA AGAGAAGCCA AAGAACCCCA TGGTAGAGTT GTTCTATGGC 
AGATTCCTGG CTGTGGGAGT ACTTGAAGGT AAAAAATTTG AAAACACTGA AATGTTTGGT 
CAGTACCCAC TTCAGGTCAA TGGGTTCAAA GATCTGCATG AGTGCCTAGA AGCTGCAATG 
ATTGAAGGAG AAATTGAGTC TTTACATTCA GAGAATTCAG GAAAATCAGG CCAAGAGCAT 
TGGTTTACTG AATTACCACC TGTGTTAACA TTTGAATTGT CAAGATTTGA ATTTAATCAG 
GCATTGGGAA GACCAGAAAA AATTCACAAC AAATTAGAAT TTCCCCAAGT TTTATATTTG 
GACAGATACA TGCACAGAAA CAGAGAAATA ACAAGAATTA AGAGGGAAGA GATCAAGAGA 
CTGAAAGATT ACCTCACGGT ATTACAACAA AGGCTAGAAA GATATTTAAG CTATGGTTCC 
GGTCCCAAAC GATTCCCCTT GGTAGATGTT CTTCAGTATG CATTGGAATT TGCCTCAAGT 
AAACCTGTTT GCACTTCTCC TGTTGACGAT ATTGACGCTA GTTCCCCACC TAGTGGTTCC 
ATACCATCAC AGACATTACC AAGCACAACA GAACAACAGG GAGCCCTATC TTCAGAACTG 
CCAAGCACAT CACCTTCATC AGTTGCTGCC ATTTCATCGA GATCAGTAAT ACACAAACCA 
TTTACTCAGT CCCGGATACC TCCAGATTTG CCCATGCATC CGGCACCAAG GCACATAACG 
GAGGAAGAAC TTTCTGTGCT GGAAAGTTGT TTACATCGCT GGAGGACAGA AATAGAAAAT 
GACACCAGAG ATTTGCAGGA AAGCATATCC AGAATCCATC GAACAATTGA ATTAATGTAC 
TCTGACAAAT CTATGATACA AGTTCCTTAT CGATTACATG CCGTTTTAGT TCACGAAGGC 
CAAGCTAATG CTGGGCACTA CTGGGCATAT ATTTTTGATC ATCGTGAAAG CAGATGGATG 
AAGTACAATG ATATTGCTGT GACAAAATCA TCATGGGAAG AGCTAGTGAG GGACTCTTTT 
GGTGGTTATA GAAATGCCAG TGCATACTGT TTAATGTACA TAAATGATAA GGCACAGTTC 
CTAATACAAG AGGAGTTTAA TAAAGAAACT GGGCAGCCCC TTGTTGGTAT AGAAACATTA 
CCACCGGATT TGAGAGATTT TGTTGAGGAA GACAACCAAC GATTTGAAAA AGAACTAGAA 
GAATGGGATG CACAACTTGC CCAGAAAGCT TTGCAGGAAA AGCTTTTAGC GTCTCAGAAA 
TTGAGAGAGT CAGAGACTTC TGTGACAACA GCACAAGCAG CAGGAGACCC AGAATATCTA 
GAGCAGCCAT CAAGAAGTGA TTTCTCAAAG CACTTGAAAG AAGAAACTAT TCAAATAATT 
ACCAAGGCAT CACATGAGCA TGAAGATAAA AGTCCTGAAA CAGTTTTGCA GTCGGCAATT 
AAGTTGGAAT ATGCAAGGTT GGTTAAGTTG GCCCAAGAAG ACACCCCACC AGAAACCGAT 
TATCGTTTAC ATCATGTAGT GGTCTACTTT ATCCAGAACC AGGCACCAAA GAAAATTATT 
GAGAAAACAT TACTAGAACA ATTTGGAGAT AGAAATTTGA GTTTTGATGA AAGGTGTCAC 
AACATAATGA AAGTTGCTCA AGCCAAACTG GAAATGATAA AACCTGAAGA AGTAAACTTG 
GAGGAATATG AGGAGTGGCA TCAGGATTAT AGGAAATTCA GGGAAACAAC TATGTATCTC 
ATAATTGGGC TAGAAAATTT TCAAAGAGAA AGTTATATAG ATTCCTTGCT GTTCCTCATC 
TGTGCTTATC AGAATAACAA AGAACTCTTG TCTAAAGGCT TATACAGAGG ACATGATGAA 
GAATTGATAT CACATTATAG AAGAGAATGT TTGCTAAAAT TAAATGAGCA AGCCGCAGAA 
CTCTTCGAAT CTGGAGAGGA TCGAGAAGTA AACAATGGTT TGATTATCAT GAATGAGTTT 
ATTGTCCCAT TTTTGCCATT ATTACTGGTG GATGAAATGG AAGAAAAGGA TATACTAGCT 
GTAGAAGATA TGAGAAATCG ATGGTGTTCC TACCTTGGTC AAGAAATGGA ACCACACCTC 
CAAGAAAAGC TGACAGATTT TTTGCCAAAA CTGCTTGATT GTTCTATGGA GATTAAAAGT 
TTCCATGAGC CACCGAAGTT ACCTTCATAT TCCACGCATG AACTCTGTGA GCGATTTGCC 
CGAATCATGT TGTCCCTCAG TCGAACTCCT GCTGATGGAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028325.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883814.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu93250
Clone ID Related Accession (Same CDS sequence)	XM_017028325.1
Accession Version	XM_017028325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGTGT 
GGTCAAAGAA TATGGCCAAT ATTATTTCAG TCCTCTGGAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883814.1
CDS	448..2832
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSCGQRIWPILFQSSGI

Target ORF information:

RefSeq Version	XM_017028325.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028325.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGTGT 
GGTCAAAGAA TATGGCCAAT ATTATTTCAG TCCTCTGGAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028328.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883817.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu93251
Clone ID Related Accession (Same CDS sequence)	XM_017028328.1
Accession Version	XM_017028328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGTTTTTGA AATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883817.1
CDS	448..2454
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQVFEI

Target ORF information:

RefSeq Version	XM_017028328.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028328.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGTTTTTGA AATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028329.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883818.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu93252
Clone ID Related Accession (Same CDS sequence)	NM_001352561.1 , XM_017028329.1 , NM_001352561.2
Accession Version	XM_017028329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGGCAAT TAAGTTGGAA 
TATGCAAGGT TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA 
CATCATGTAG TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA 
TTACTAGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT 
GAGGAGTGGC ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT 
CAGAATAACA AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA 
TCTGGAGAGG ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA 
TTTTTGCCAT TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT 
ATGAGAAATC GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG 
CTGACAGATT TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG 
CCACCGAAGT TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG 
TTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883818.1
CDS	594..2549
Translation	MHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028329.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028329.1

ORF Insert Sequence:

ATGCACAGAA ACAGAGAAAT AACAAGAATT AAGAGGGAAG AGATCAAGAG ACTGAAAGAT 
TACCTCACGG TATTACAACA AAGGCTAGAA AGATATTTAA GCTATGGTTC CGGTCCCAAA 
CGATTCCCCT TGGTAGATGT TCTTCAGTAT GCATTGGAAT TTGCCTCAAG TAAACCTGTT 
TGCACTTCTC CTGTTGACGA TATTGACGCT AGTTCCCCAC CTAGTGGTTC CATACCATCA 
CAGACATTAC CAAGCACAAC AGAACAACAG GGAGCCCTAT CTTCAGAACT GCCAAGCACA 
TCACCTTCAT CAGTTGCTGC CATTTCATCG AGATCAGTAA TACACAAACC ATTTACTCAG 
TCCCGGATAC CTCCAGATTT GCCCATGCAT CCGGCACCAA GGCACATAAC GGAGGAAGAA 
CTTTCTGTGC TGGAAAGTTG TTTACATCGC TGGAGGACAG AAATAGAAAA TGACACCAGA 
GATTTGCAGG AAAGCATATC CAGAATCCAT CGAACAATTG AATTAATGTA CTCTGACAAA 
TCTATGATAC AAGTTCCTTA TCGATTACAT GCCGTTTTAG TTCACGAAGG CCAAGCTAAT 
GCTGGGCACT ACTGGGCATA TATTTTTGAT CATCGTGAAA GCAGATGGAT GAAGTACAAT 
GATATTGCTG TGACAAAATC ATCATGGGAA GAGCTAGTGA GGGACTCTTT TGGTGGTTAT 
AGAAATGCCA GTGCATACTG TTTAATGTAC ATAAATGATA AGGCACAGTT CCTAATACAA 
GAGGAGTTTA ATAAAGAAAC TGGGCAGCCC CTTGTTGGTA TAGAAACATT ACCACCGGAT 
TTGAGAGATT TTGTTGAGGA AGACAACCAA CGATTTGAAA AAGAACTAGA AGAATGGGAT 
GCACAACTTG CCCAGAAAGC TTTGCAGGAA AAGCTTTTAG CGTCTCAGAA ATTGAGAGAG 
TCAGAGACTT CTGTGACAAC AGCACAAGCA GCAGGAGACC CAGAATATCT AGAGCAGCCA 
TCAAGAAGTG ATTTCTCAAA GCACTTGAAA GAAGAAACTA TTCAAATAAT TACCAAGGCA 
TCACATGAGC ATGAAGATAA AAGTCCTGAA ACAGTTTTGC AGTCGGCAAT TAAGTTGGAA 
TATGCAAGGT TGGTTAAGTT GGCCCAAGAA GACACCCCAC CAGAAACCGA TTATCGTTTA 
CATCATGTAG TGGTCTACTT TATCCAGAAC CAGGCACCAA AGAAAATTAT TGAGAAAACA 
TTACTAGAAC AATTTGGAGA TAGAAATTTG AGTTTTGATG AAAGGTGTCA CAACATAATG 
AAAGTTGCTC AAGCCAAACT GGAAATGATA AAACCTGAAG AAGTAAACTT GGAGGAATAT 
GAGGAGTGGC ATCAGGATTA TAGGAAATTC AGGGAAACAA CTATGTATCT CATAATTGGG 
CTAGAAAATT TTCAAAGAGA AAGTTATATA GATTCCTTGC TGTTCCTCAT CTGTGCTTAT 
CAGAATAACA AAGAACTCTT GTCTAAAGGC TTATACAGAG GACATGATGA AGAATTGATA 
TCACATTATA GAAGAGAATG TTTGCTAAAA TTAAATGAGC AAGCCGCAGA ACTCTTCGAA 
TCTGGAGAGG ATCGAGAAGT AAACAATGGT TTGATTATCA TGAATGAGTT TATTGTCCCA 
TTTTTGCCAT TATTACTGGT GGATGAAATG GAAGAAAAGG ATATACTAGC TGTAGAAGAT 
ATGAGAAATC GATGGTGTTC CTACCTTGGT CAAGAAATGG AACCACACCT CCAAGAAAAG 
CTGACAGATT TTTTGCCAAA ACTGCTTGAT TGTTCTATGG AGATTAAAAG TTTCCATGAG 
CCACCGAAGT TACCTTCATA TTCCACGCAT GAACTCTGTG AGCGATTTGC CCGAATCATG 
TTGTCCCTCA GTCGAACTCC TGCTGATGGA AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( XM_017028321.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883810.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

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CloneID	OHu93248
Clone ID Related Accession (Same CDS sequence)	XM_017028321.1
Accession Version	XM_017028321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATGA CAGATAGCAT TTTTGAAAGT GGTGGTGCCT TTGAAACTGC ATCCTTACTC 
CTTTTGTCAC TGACTGCTAC AACTGGAATT GGGGATAGTA ATGGAAACTT GGAATTAGCA 
GTGGCTTTCC TTACTGCGAA GAATGCTAAG ACCCCTCAGC AGGAGGAGAC AACTTACTAC 
CAAACAGCAC TTCCTGGCAA TGATAGATAC ATCAGTGTGG GAAGCCAAGC AGATACAAAT 
GTGATTGATC TCACTGGAGA TGATAAAGAT GATCTTCAGA GAGCAATTGC CTTGAGTTTG 
GCCGAATCAA ACAGGGCATT CAGGGAGACT GGAATAACTG ATGAGGAACA AGCCATTAGC 
AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT AAAGCATGTT TGAAGAGGAC ACCTACAGAA 
GTTTGGAGGG ATTCTCGAAA CCCTTATGAT AGAAAAAGAC AGGACAAAGC TCCCGTTGGG 
CTAAAGAATG TTGGCAATAC TTGTTGGTTT AGTGCTGTTA TTCAGTCATT ATTTAATCTT 
TTGGAATTTA GAAGATTAGT TCTGAATTAC AAGCCTCCAT CAAATGCTCA AGATTTACCC 
CGAAACCAAA AGGAACATCG GAATTTGCCT TTTATGCGTG AGCTGAGGTA TCTATTTGCA 
CTTCTTGTTG GTACCAAAAG GAAGTATGTT GATCCATCAA GAGCAGTTGA AATTCTTAAG 
GATGCTTTCA AATCAAATGA CTCACAGCAG CAAGATGTGA GTGAGTTTAC ACACAAATTA 
TTAGATTGGT TAGAAGATGC CTTCCAAATG AAAGCTGAAG AGGAGACGGA TGAAGAGAAG 
CCAAAGAACC CCATGGTAGA GTTGTTCTAT GGCAGATTCC TGGCTGTGGG AGTACTTGAA 
GGTAAAAAAT TTGAAAACAC TGAAATGTTT GGTCAGTACC CACTTCAGGT CAATGGGTTC 
AAAGATCTGC ATGAGTGCCT AGAAGCTGCA ATGATTGAAG GAGAAATTGA GTCTTTACAT 
TCAGAGAATT CAGGAAAATC AGGCCAAGAG CATTGGTTTA CTGAATTACC ACCTGTGTTA 
ACATTTGAAT TGTCAAGATT TGAATTTAAT CAGGCATTGG GAAGACCAGA AAAAATTCAC 
AACAAATTAG AATTTCCCCA AGTTTTATAT TTGGACAGAT ACATGCACAG AAACAGAGAA 
ATAACAAGAA TTAAGAGGGA AGAGATCAAG AGACTGAAAG ATTACCTCAC GGTATTACAA 
CAAAGGCTAG AAAGATATTT AAGCTATGGT TCCGGTCCCA AACGATTCCC CTTGGTAGAT 
GTTCTTCAGT ATGCATTGGA ATTTGCCTCA AGTAAACCTG TTTGCACTTC TCCTGTTGAC 
GATATTGACG CTAGTTCCCC ACCTAGTGGT TCCATACCAT CACAGACATT ACCAAGCACA 
ACAGAACAAC AGGGAGCCCT ATCTTCAGAA CTGCCAAGCA CATCACCTTC ATCAGTTGCT 
GCCATTTCAT CGAGATCAGT AATACACAAA CCATTTACTC AGTCCCGGAT ACCTCCAGAT 
TTGCCCATGC ATCCGGCACC AAGGCACATA ACGGAGGAAG AACTTTCTGT GCTGGAAAGT 
TGTTTACATC GCTGGAGGAC AGAAATAGAA AATGACACCA GAGATTTGCA GGAAAGCATA 
TCCAGAATCC ATCGAACAAT TGAATTAATG TACTCTGACA AATCTATGAT ACAAGTTCCT 
TATCGATTAC ATGCCGTTTT AGTTCACGAA GGCCAAGCTA ATGCTGGGCA CTACTGGGCA 
TATATTTTTG ATCATCGTGA AAGCAGATGG ATGAAGTACA ATGATATTGC TGTGACAAAA 
TCATCATGGG AAGAGCTAGT GAGGGACTCT TTTGGTGGTT ATAGAAATGC CAGTGCATAC 
TGTTTAATGT ACATAAATGA TAAGGCACAG TTCCTAATAC AAGAGGAGTT TAATAAAGAA 
ACTGGGCAGC CCCTTGTTGG TATAGAAACA TTACCACCGG ATTTGAGAGA TTTTGTTGAG 
GAAGACAACC AACGATTTGA AAAAGAACTA GAAGAATGGG ATGCACAACT TGCCCAGAAA 
GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG AAATTGAGAG AGTCAGAGAC TTCTGTGACA 
ACAGCACAAG CAGCAGGAGA CCCAGAATAT CTAGAGCAGC CATCAAGAAG TGATTTCTCA 
AAGCACTTGA AAGAAGAAAC TATTCAAATA ATTACCAAGG CATCACATGA GCATGAAGAT 
AAAAGTCCTG AAACAGTTTT GCAGTCGAAA CCTGAAAATA CTACAAGCCA ACCACTTTCT 
AATCAGCGAG TTGTAGAGGT GGCGATCCCT CATGTAGGGA AATTTATGAT TGAATCAAAG 
GAGGGGGGGT ATGATGACGA GATCATGATG ACACCGAACA TGCAAGGTAT TATCATGGCG 
ATAGGTAAAT CCAGGAGTGT ATATGACAGG TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883810.1
CDS	81..3428
Translation	MIMTDSIFESGGAFETASLLLLSLTATTGIGDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	XM_017028321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028321.1

ORF Insert Sequence:

ATGATCATGA CAGATAGCAT TTTTGAAAGT GGTGGTGCCT TTGAAACTGC ATCCTTACTC 
CTTTTGTCAC TGACTGCTAC AACTGGAATT GGGGATAGTA ATGGAAACTT GGAATTAGCA 
GTGGCTTTCC TTACTGCGAA GAATGCTAAG ACCCCTCAGC AGGAGGAGAC AACTTACTAC 
CAAACAGCAC TTCCTGGCAA TGATAGATAC ATCAGTGTGG GAAGCCAAGC AGATACAAAT 
GTGATTGATC TCACTGGAGA TGATAAAGAT GATCTTCAGA GAGCAATTGC CTTGAGTTTG 
GCCGAATCAA ACAGGGCATT CAGGGAGACT GGAATAACTG ATGAGGAACA AGCCATTAGC 
AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT AAAGCATGTT TGAAGAGGAC ACCTACAGAA 
GTTTGGAGGG ATTCTCGAAA CCCTTATGAT AGAAAAAGAC AGGACAAAGC TCCCGTTGGG 
CTAAAGAATG TTGGCAATAC TTGTTGGTTT AGTGCTGTTA TTCAGTCATT ATTTAATCTT 
TTGGAATTTA GAAGATTAGT TCTGAATTAC AAGCCTCCAT CAAATGCTCA AGATTTACCC 
CGAAACCAAA AGGAACATCG GAATTTGCCT TTTATGCGTG AGCTGAGGTA TCTATTTGCA 
CTTCTTGTTG GTACCAAAAG GAAGTATGTT GATCCATCAA GAGCAGTTGA AATTCTTAAG 
GATGCTTTCA AATCAAATGA CTCACAGCAG CAAGATGTGA GTGAGTTTAC ACACAAATTA 
TTAGATTGGT TAGAAGATGC CTTCCAAATG AAAGCTGAAG AGGAGACGGA TGAAGAGAAG 
CCAAAGAACC CCATGGTAGA GTTGTTCTAT GGCAGATTCC TGGCTGTGGG AGTACTTGAA 
GGTAAAAAAT TTGAAAACAC TGAAATGTTT GGTCAGTACC CACTTCAGGT CAATGGGTTC 
AAAGATCTGC ATGAGTGCCT AGAAGCTGCA ATGATTGAAG GAGAAATTGA GTCTTTACAT 
TCAGAGAATT CAGGAAAATC AGGCCAAGAG CATTGGTTTA CTGAATTACC ACCTGTGTTA 
ACATTTGAAT TGTCAAGATT TGAATTTAAT CAGGCATTGG GAAGACCAGA AAAAATTCAC 
AACAAATTAG AATTTCCCCA AGTTTTATAT TTGGACAGAT ACATGCACAG AAACAGAGAA 
ATAACAAGAA TTAAGAGGGA AGAGATCAAG AGACTGAAAG ATTACCTCAC GGTATTACAA 
CAAAGGCTAG AAAGATATTT AAGCTATGGT TCCGGTCCCA AACGATTCCC CTTGGTAGAT 
GTTCTTCAGT ATGCATTGGA ATTTGCCTCA AGTAAACCTG TTTGCACTTC TCCTGTTGAC 
GATATTGACG CTAGTTCCCC ACCTAGTGGT TCCATACCAT CACAGACATT ACCAAGCACA 
ACAGAACAAC AGGGAGCCCT ATCTTCAGAA CTGCCAAGCA CATCACCTTC ATCAGTTGCT 
GCCATTTCAT CGAGATCAGT AATACACAAA CCATTTACTC AGTCCCGGAT ACCTCCAGAT 
TTGCCCATGC ATCCGGCACC AAGGCACATA ACGGAGGAAG AACTTTCTGT GCTGGAAAGT 
TGTTTACATC GCTGGAGGAC AGAAATAGAA AATGACACCA GAGATTTGCA GGAAAGCATA 
TCCAGAATCC ATCGAACAAT TGAATTAATG TACTCTGACA AATCTATGAT ACAAGTTCCT 
TATCGATTAC ATGCCGTTTT AGTTCACGAA GGCCAAGCTA ATGCTGGGCA CTACTGGGCA 
TATATTTTTG ATCATCGTGA AAGCAGATGG ATGAAGTACA ATGATATTGC TGTGACAAAA 
TCATCATGGG AAGAGCTAGT GAGGGACTCT TTTGGTGGTT ATAGAAATGC CAGTGCATAC 
TGTTTAATGT ACATAAATGA TAAGGCACAG TTCCTAATAC AAGAGGAGTT TAATAAAGAA 
ACTGGGCAGC CCCTTGTTGG TATAGAAACA TTACCACCGG ATTTGAGAGA TTTTGTTGAG 
GAAGACAACC AACGATTTGA AAAAGAACTA GAAGAATGGG ATGCACAACT TGCCCAGAAA 
GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG AAATTGAGAG AGTCAGAGAC TTCTGTGACA 
ACAGCACAAG CAGCAGGAGA CCCAGAATAT CTAGAGCAGC CATCAAGAAG TGATTTCTCA 
AAGCACTTGA AAGAAGAAAC TATTCAAATA ATTACCAAGG CATCACATGA GCATGAAGAT 
AAAAGTCCTG AAACAGTTTT GCAGTCGAAA CCTGAAAATA CTACAAGCCA ACCACTTTCT 
AATCAGCGAG TTGTAGAGGT GGCGATCCCT CATGTAGGGA AATTTATGAT TGAATCAAAG 
GAGGGGGGGT ATGATGACGA GATCATGATG ACACCGAACA TGCAAGGTAT TATCATGGCG 
ATAGGTAAAT CCAGGAGTGT ATATGACAGG TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001283042.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269971.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.

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CloneID	OHu11796
Clone ID Related Accession (Same CDS sequence)	NM_001283042.3 , NM_001283042.1 , NM_001283042.2
Accession Version	NM_001283042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, BC075792.1, AF170562.1, AJ010598.1 and BM149594.1. On Jun 2, 2019 this sequence version replaced NM_001283042.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (USP25b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF170562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGATC 
ATGATGACAC CGAACATGCA AGGTATTATC ATGGCGATAG GTAAATCCAG GAGTGTATAT 
GACAGGTGTG GCCCTGAAGC AGGGTTCTTT AAGGCAATTA AGTTGGAATA TGCAAGGTTG 
GTTAAGTTGG CCCAAGAAGA CACCCCACCA GAAACCGATT ATCGTTTACA TCATGTAGTG 
GTCTACTTTA TCCAGAACCA GGCACCAAAG AAAATTATTG AGAAAACATT ACTAGAACAA 
TTTGGAGATA GAAATTTGAG TTTTGATGAA AGGTGTCACA ACATAATGAA AGTTGCTCAA 
GCCAAACTGG AAATGATAAA ACCTGAAGAA GTAAACTTGG AGGAATATGA GGAGTGGCAT 
CAGGATTATA GGAAATTCAG GGAAACAACT ATGTATCTCA TAATTGGGCT AGAAAATTTT 
CAAAGAGAAA GTTATATAGA TTCCTTGCTG TTCCTCATCT GTGCTTATCA GAATAACAAA 
GAACTCTTGT CTAAAGGCTT ATACAGAGGA CATGATGAAG AATTGATATC ACATTATAGA 
AGAGAATGTT TGCTAAAATT AAATGAGCAA GCCGCAGAAC TCTTCGAATC TGGAGAGGAT 
CGAGAAGTAA ACAATGGTTT GATTATCATG AATGAGTTTA TTGTCCCATT TTTGCCATTA 
TTACTGGTGG ATGAAATGGA AGAAAAGGAT ATACTAGCTG TAGAAGATAT GAGAAATCGA 
TGGTGTTCCT ACCTTGGTCA AGAAATGGAA CCACACCTCC AAGAAAAGCT GACAGATTTT 
TTGCCAAAAC TGCTTGATTG TTCTATGGAG ATTAAAAGTT TCCATGAGCC ACCGAAGTTA 
CCTTCATATT CCACGCATGA ACTCTGTGAG CGATTTGCCC GAATCATGTT GTCCCTCAGT 
CGAACTCCTG CTGATGGAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269971.1
CDS	413..3676
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001283042.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283042.3

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGATC 
ATGATGACAC CGAACATGCA AGGTATTATC ATGGCGATAG GTAAATCCAG GAGTGTATAT 
GACAGGTGTG GCCCTGAAGC AGGGTTCTTT AAGGCAATTA AGTTGGAATA TGCAAGGTTG 
GTTAAGTTGG CCCAAGAAGA CACCCCACCA GAAACCGATT ATCGTTTACA TCATGTAGTG 
GTCTACTTTA TCCAGAACCA GGCACCAAAG AAAATTATTG AGAAAACATT ACTAGAACAA 
TTTGGAGATA GAAATTTGAG TTTTGATGAA AGGTGTCACA ACATAATGAA AGTTGCTCAA 
GCCAAACTGG AAATGATAAA ACCTGAAGAA GTAAACTTGG AGGAATATGA GGAGTGGCAT 
CAGGATTATA GGAAATTCAG GGAAACAACT ATGTATCTCA TAATTGGGCT AGAAAATTTT 
CAAAGAGAAA GTTATATAGA TTCCTTGCTG TTCCTCATCT GTGCTTATCA GAATAACAAA 
GAACTCTTGT CTAAAGGCTT ATACAGAGGA CATGATGAAG AATTGATATC ACATTATAGA 
AGAGAATGTT TGCTAAAATT AAATGAGCAA GCCGCAGAAC TCTTCGAATC TGGAGAGGAT 
CGAGAAGTAA ACAATGGTTT GATTATCATG AATGAGTTTA TTGTCCCATT TTTGCCATTA 
TTACTGGTGG ATGAAATGGA AGAAAAGGAT ATACTAGCTG TAGAAGATAT GAGAAATCGA 
TGGTGTTCCT ACCTTGGTCA AGAAATGGAA CCACACCTCC AAGAAAAGCT GACAGATTTT 
TTGCCAAAAC TGCTTGATTG TTCTATGGAG ATTAAAAGTT TCCATGAGCC ACCGAAGTTA 
CCTTCATATT CCACGCATGA ACTCTGTGAG CGATTTGCCC GAATCATGTT GTCCCTCAGT 
CGAACTCCTG CTGATGGAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001283042.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269971.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.

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CloneID	OHu11796
Clone ID Related Accession (Same CDS sequence)	NM_001283042.3 , NM_001283042.1 , NM_001283042.2
Accession Version	NM_001283042.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-06
Organism	Homo sapiens(Human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25b
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075792.1, AF170562.1, AJ010598.1 and BM149594.1. On Sep 25, 2013 this sequence version replaced XM_005260948.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (USP25b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF170562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGATC 
ATGATGACAC CGAACATGCA AGGTATTATC ATGGCGATAG GTAAATCCAG GAGTGTATAT 
GACAGGTGTG GCCCTGAAGC AGGGTTCTTT AAGGCAATTA AGTTGGAATA TGCAAGGTTG 
GTTAAGTTGG CCCAAGAAGA CACCCCACCA GAAACCGATT ATCGTTTACA TCATGTAGTG 
GTCTACTTTA TCCAGAACCA GGCACCAAAG AAAATTATTG AGAAAACATT ACTAGAACAA 
TTTGGAGATA GAAATTTGAG TTTTGATGAA AGGTGTCACA ACATAATGAA AGTTGCTCAA 
GCCAAACTGG AAATGATAAA ACCTGAAGAA GTAAACTTGG AGGAATATGA GGAGTGGCAT 
CAGGATTATA GGAAATTCAG GGAAACAACT ATGTATCTCA TAATTGGGCT AGAAAATTTT 
CAAAGAGAAA GTTATATAGA TTCCTTGCTG TTCCTCATCT GTGCTTATCA GAATAACAAA 
GAACTCTTGT CTAAAGGCTT ATACAGAGGA CATGATGAAG AATTGATATC ACATTATAGA 
AGAGAATGTT TGCTAAAATT AAATGAGCAA GCCGCAGAAC TCTTCGAATC TGGAGAGGAT 
CGAGAAGTAA ACAATGGTTT GATTATCATG AATGAGTTTA TTGTCCCATT TTTGCCATTA 
TTACTGGTGG ATGAAATGGA AGAAAAGGAT ATACTAGCTG TAGAAGATAT GAGAAATCGA 
TGGTGTTCCT ACCTTGGTCA AGAAATGGAA CCACACCTCC AAGAAAAGCT GACAGATTTT 
TTGCCAAAAC TGCTTGATTG TTCTATGGAG ATTAAAAGTT TCCATGAGCC ACCGAAGTTA 
CCTTCATATT CCACGCATGA ACTCTGTGAG CGATTTGCCC GAATCATGTT GTCCCTCAGT 
CGAACTCCTG CTGATGGAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269971.1
CDS	370..3633
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001283042.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283042.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGATC 
ATGATGACAC CGAACATGCA AGGTATTATC ATGGCGATAG GTAAATCCAG GAGTGTATAT 
GACAGGTGTG GCCCTGAAGC AGGGTTCTTT AAGGCAATTA AGTTGGAATA TGCAAGGTTG 
GTTAAGTTGG CCCAAGAAGA CACCCCACCA GAAACCGATT ATCGTTTACA TCATGTAGTG 
GTCTACTTTA TCCAGAACCA GGCACCAAAG AAAATTATTG AGAAAACATT ACTAGAACAA 
TTTGGAGATA GAAATTTGAG TTTTGATGAA AGGTGTCACA ACATAATGAA AGTTGCTCAA 
GCCAAACTGG AAATGATAAA ACCTGAAGAA GTAAACTTGG AGGAATATGA GGAGTGGCAT 
CAGGATTATA GGAAATTCAG GGAAACAACT ATGTATCTCA TAATTGGGCT AGAAAATTTT 
CAAAGAGAAA GTTATATAGA TTCCTTGCTG TTCCTCATCT GTGCTTATCA GAATAACAAA 
GAACTCTTGT CTAAAGGCTT ATACAGAGGA CATGATGAAG AATTGATATC ACATTATAGA 
AGAGAATGTT TGCTAAAATT AAATGAGCAA GCCGCAGAAC TCTTCGAATC TGGAGAGGAT 
CGAGAAGTAA ACAATGGTTT GATTATCATG AATGAGTTTA TTGTCCCATT TTTGCCATTA 
TTACTGGTGG ATGAAATGGA AGAAAAGGAT ATACTAGCTG TAGAAGATAT GAGAAATCGA 
TGGTGTTCCT ACCTTGGTCA AGAAATGGAA CCACACCTCC AAGAAAAGCT GACAGATTTT 
TTGCCAAAAC TGCTTGATTG TTCTATGGAG ATTAAAAGTT TCCATGAGCC ACCGAAGTTA 
CCTTCATATT CCACGCATGA ACTCTGTGAG CGATTTGCCC GAATCATGTT GTCCCTCAGT 
CGAACTCCTG CTGATGGAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001283042.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269971.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11796
Clone ID Related Accession (Same CDS sequence)	NM_001283042.3 , NM_001283042.1 , NM_001283042.2
Accession Version	NM_001283042.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-05
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, BC075792.1, AF170562.1, AJ010598.1 and BM149594.1. On Jun 18, 2017 this sequence version replaced NM_001283042.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (USP25b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF170562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGATC 
ATGATGACAC CGAACATGCA AGGTATTATC ATGGCGATAG GTAAATCCAG GAGTGTATAT 
GACAGGTGTG GCCCTGAAGC AGGGTTCTTT AAGGCAATTA AGTTGGAATA TGCAAGGTTG 
GTTAAGTTGG CCCAAGAAGA CACCCCACCA GAAACCGATT ATCGTTTACA TCATGTAGTG 
GTCTACTTTA TCCAGAACCA GGCACCAAAG AAAATTATTG AGAAAACATT ACTAGAACAA 
TTTGGAGATA GAAATTTGAG TTTTGATGAA AGGTGTCACA ACATAATGAA AGTTGCTCAA 
GCCAAACTGG AAATGATAAA ACCTGAAGAA GTAAACTTGG AGGAATATGA GGAGTGGCAT 
CAGGATTATA GGAAATTCAG GGAAACAACT ATGTATCTCA TAATTGGGCT AGAAAATTTT 
CAAAGAGAAA GTTATATAGA TTCCTTGCTG TTCCTCATCT GTGCTTATCA GAATAACAAA 
GAACTCTTGT CTAAAGGCTT ATACAGAGGA CATGATGAAG AATTGATATC ACATTATAGA 
AGAGAATGTT TGCTAAAATT AAATGAGCAA GCCGCAGAAC TCTTCGAATC TGGAGAGGAT 
CGAGAAGTAA ACAATGGTTT GATTATCATG AATGAGTTTA TTGTCCCATT TTTGCCATTA 
TTACTGGTGG ATGAAATGGA AGAAAAGGAT ATACTAGCTG TAGAAGATAT GAGAAATCGA 
TGGTGTTCCT ACCTTGGTCA AGAAATGGAA CCACACCTCC AAGAAAAGCT GACAGATTTT 
TTGCCAAAAC TGCTTGATTG TTCTATGGAG ATTAAAAGTT TCCATGAGCC ACCGAAGTTA 
CCTTCATATT CCACGCATGA ACTCTGTGAG CGATTTGCCC GAATCATGTT GTCCCTCAGT 
CGAACTCCTG CTGATGGAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269971.1
CDS	461..3724
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001283042.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283042.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGATC 
ATGATGACAC CGAACATGCA AGGTATTATC ATGGCGATAG GTAAATCCAG GAGTGTATAT 
GACAGGTGTG GCCCTGAAGC AGGGTTCTTT AAGGCAATTA AGTTGGAATA TGCAAGGTTG 
GTTAAGTTGG CCCAAGAAGA CACCCCACCA GAAACCGATT ATCGTTTACA TCATGTAGTG 
GTCTACTTTA TCCAGAACCA GGCACCAAAG AAAATTATTG AGAAAACATT ACTAGAACAA 
TTTGGAGATA GAAATTTGAG TTTTGATGAA AGGTGTCACA ACATAATGAA AGTTGCTCAA 
GCCAAACTGG AAATGATAAA ACCTGAAGAA GTAAACTTGG AGGAATATGA GGAGTGGCAT 
CAGGATTATA GGAAATTCAG GGAAACAACT ATGTATCTCA TAATTGGGCT AGAAAATTTT 
CAAAGAGAAA GTTATATAGA TTCCTTGCTG TTCCTCATCT GTGCTTATCA GAATAACAAA 
GAACTCTTGT CTAAAGGCTT ATACAGAGGA CATGATGAAG AATTGATATC ACATTATAGA 
AGAGAATGTT TGCTAAAATT AAATGAGCAA GCCGCAGAAC TCTTCGAATC TGGAGAGGAT 
CGAGAAGTAA ACAATGGTTT GATTATCATG AATGAGTTTA TTGTCCCATT TTTGCCATTA 
TTACTGGTGG ATGAAATGGA AGAAAAGGAT ATACTAGCTG TAGAAGATAT GAGAAATCGA 
TGGTGTTCCT ACCTTGGTCA AGAAATGGAA CCACACCTCC AAGAAAAGCT GACAGATTTT 
TTGCCAAAAC TGCTTGATTG TTCTATGGAG ATTAAAAGTT TCCATGAGCC ACCGAAGTTA 
CCTTCATATT CCACGCATGA ACTCTGTGAG CGATTTGCCC GAATCATGTT GTCCCTCAGT 
CGAACTCCTG CTGATGGAAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001283041.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269970.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12464
Clone ID Related Accession (Same CDS sequence)	NM_001283041.1 , NM_001283041.2
Accession Version	NM_001283041.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-06
Organism	Homo sapiens(Human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25m
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075792.1, FJ763652.1, AJ010598.1 and BM149594.1. On Sep 25, 2013 this sequence version replaced XM_005260946.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (USP25m). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ763652.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGAAA 
CCTGAAAATA CTACAAGCCA ACCACTTTCT AATCAGCGAG TTGTAGAGGT GGCGATCCCT 
CATGTAGGGA AATTTATGAT TGAATCAAAG GAGGGGGGGT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AATATGCAAG GTTGGTTAAG 
TTGGCCCAAG AAGACACCCC ACCAGAAACC GATTATCGTT TACATCATGT AGTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATT ATTGAGAAAA CATTACTAGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TAAAACCTGA AGAAGTAAAC TTGGAGGAAT ATGAGGAGTG GCATCAGGAT 
TATAGGAAAT TCAGGGAAAC AACTATGTAT CTCATAATTG GGCTAGAAAA TTTTCAAAGA 
GAAAGTTATA TAGATTCCTT GCTGTTCCTC ATCTGTGCTT ATCAGAATAA CAAAGAACTC 
TTGTCTAAAG GCTTATACAG AGGACATGAT GAAGAATTGA TATCACATTA TAGAAGAGAA 
TGTTTGCTAA AATTAAATGA GCAAGCCGCA GAACTCTTCG AATCTGGAGA GGATCGAGAA 
GTAAACAATG GTTTGATTAT CATGAATGAG TTTATTGTCC CATTTTTGCC ATTATTACTG 
GTGGATGAAA TGGAAGAAAA GGATATACTA GCTGTAGAAG ATATGAGAAA TCGATGGTGT 
TCCTACCTTG GTCAAGAAAT GGAACCACAC CTCCAAGAAA AGCTGACAGA TTTTTTGCCA 
AAACTGCTTG ATTGTTCTAT GGAGATTAAA AGTTTCCATG AGCCACCGAA GTTACCTTCA 
TATTCCACGC ATGAACTCTG TGAGCGATTT GCCCGAATCA TGTTGTCCCT CAGTCGAACT 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269970.1
CDS	370..3747
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001283041.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283041.1

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGAAA 
CCTGAAAATA CTACAAGCCA ACCACTTTCT AATCAGCGAG TTGTAGAGGT GGCGATCCCT 
CATGTAGGGA AATTTATGAT TGAATCAAAG GAGGGGGGGT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AATATGCAAG GTTGGTTAAG 
TTGGCCCAAG AAGACACCCC ACCAGAAACC GATTATCGTT TACATCATGT AGTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATT ATTGAGAAAA CATTACTAGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TAAAACCTGA AGAAGTAAAC TTGGAGGAAT ATGAGGAGTG GCATCAGGAT 
TATAGGAAAT TCAGGGAAAC AACTATGTAT CTCATAATTG GGCTAGAAAA TTTTCAAAGA 
GAAAGTTATA TAGATTCCTT GCTGTTCCTC ATCTGTGCTT ATCAGAATAA CAAAGAACTC 
TTGTCTAAAG GCTTATACAG AGGACATGAT GAAGAATTGA TATCACATTA TAGAAGAGAA 
TGTTTGCTAA AATTAAATGA GCAAGCCGCA GAACTCTTCG AATCTGGAGA GGATCGAGAA 
GTAAACAATG GTTTGATTAT CATGAATGAG TTTATTGTCC CATTTTTGCC ATTATTACTG 
GTGGATGAAA TGGAAGAAAA GGATATACTA GCTGTAGAAG ATATGAGAAA TCGATGGTGT 
TCCTACCTTG GTCAAGAAAT GGAACCACAC CTCCAAGAAA AGCTGACAGA TTTTTTGCCA 
AAACTGCTTG ATTGTTCTAT GGAGATTAAA AGTTTCCATG AGCCACCGAA GTTACCTTCA 
TATTCCACGC ATGAACTCTG TGAGCGATTT GCCCGAATCA TGTTGTCCCT CAGTCGAACT 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_001283041.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269970.1 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 1, mRNA.

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CloneID	OHu12464
Clone ID Related Accession (Same CDS sequence)	NM_001283041.1 , NM_001283041.2
Accession Version	NM_001283041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25m
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, BC075792.1, FJ763652.1, AJ010598.1 and BM149594.1. On Jun 18, 2017 this sequence version replaced NM_001283041.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (USP25m). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ763652.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGAAA 
CCTGAAAATA CTACAAGCCA ACCACTTTCT AATCAGCGAG TTGTAGAGGT GGCGATCCCT 
CATGTAGGGA AATTTATGAT TGAATCAAAG GAGGGGGGGT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AATATGCAAG GTTGGTTAAG 
TTGGCCCAAG AAGACACCCC ACCAGAAACC GATTATCGTT TACATCATGT AGTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATT ATTGAGAAAA CATTACTAGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TAAAACCTGA AGAAGTAAAC TTGGAGGAAT ATGAGGAGTG GCATCAGGAT 
TATAGGAAAT TCAGGGAAAC AACTATGTAT CTCATAATTG GGCTAGAAAA TTTTCAAAGA 
GAAAGTTATA TAGATTCCTT GCTGTTCCTC ATCTGTGCTT ATCAGAATAA CAAAGAACTC 
TTGTCTAAAG GCTTATACAG AGGACATGAT GAAGAATTGA TATCACATTA TAGAAGAGAA 
TGTTTGCTAA AATTAAATGA GCAAGCCGCA GAACTCTTCG AATCTGGAGA GGATCGAGAA 
GTAAACAATG GTTTGATTAT CATGAATGAG TTTATTGTCC CATTTTTGCC ATTATTACTG 
GTGGATGAAA TGGAAGAAAA GGATATACTA GCTGTAGAAG ATATGAGAAA TCGATGGTGT 
TCCTACCTTG GTCAAGAAAT GGAACCACAC CTCCAAGAAA AGCTGACAGA TTTTTTGCCA 
AAACTGCTTG ATTGTTCTAT GGAGATTAAA AGTTTCCATG AGCCACCGAA GTTACCTTCA 
TATTCCACGC ATGAACTCTG TGAGCGATTT GCCCGAATCA TGTTGTCCCT CAGTCGAACT 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269970.1
CDS	461..3838
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_001283041.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283041.2

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGAAA 
CCTGAAAATA CTACAAGCCA ACCACTTTCT AATCAGCGAG TTGTAGAGGT GGCGATCCCT 
CATGTAGGGA AATTTATGAT TGAATCAAAG GAGGGGGGGT ATGATGACGA GATCATGATG 
ACACCGAACA TGCAAGGTAT TATCATGGCG ATAGGTAAAT CCAGGAGTGT ATATGACAGG 
TGTGGCCCTG AAGCAGGGTT CTTTAAGGCA ATTAAGTTGG AATATGCAAG GTTGGTTAAG 
TTGGCCCAAG AAGACACCCC ACCAGAAACC GATTATCGTT TACATCATGT AGTGGTCTAC 
TTTATCCAGA ACCAGGCACC AAAGAAAATT ATTGAGAAAA CATTACTAGA ACAATTTGGA 
GATAGAAATT TGAGTTTTGA TGAAAGGTGT CACAACATAA TGAAAGTTGC TCAAGCCAAA 
CTGGAAATGA TAAAACCTGA AGAAGTAAAC TTGGAGGAAT ATGAGGAGTG GCATCAGGAT 
TATAGGAAAT TCAGGGAAAC AACTATGTAT CTCATAATTG GGCTAGAAAA TTTTCAAAGA 
GAAAGTTATA TAGATTCCTT GCTGTTCCTC ATCTGTGCTT ATCAGAATAA CAAAGAACTC 
TTGTCTAAAG GCTTATACAG AGGACATGAT GAAGAATTGA TATCACATTA TAGAAGAGAA 
TGTTTGCTAA AATTAAATGA GCAAGCCGCA GAACTCTTCG AATCTGGAGA GGATCGAGAA 
GTAAACAATG GTTTGATTAT CATGAATGAG TTTATTGTCC CATTTTTGCC ATTATTACTG 
GTGGATGAAA TGGAAGAAAA GGATATACTA GCTGTAGAAG ATATGAGAAA TCGATGGTGT 
TCCTACCTTG GTCAAGAAAT GGAACCACAC CTCCAAGAAA AGCTGACAGA TTTTTTGCCA 
AAACTGCTTG ATTGTTCTAT GGAGATTAAA AGTTTCCATG AGCCACCGAA GTTACCTTCA 
TATTCCACGC ATGAACTCTG TGAGCGATTT GCCCGAATCA TGTTGTCCCT CAGTCGAACT 
CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_013396.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037528.3 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.

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CloneID	OHu12469
Clone ID Related Accession (Same CDS sequence)	NM_013396.4 , NM_013396.6 , NM_013396.5
Accession Version	NM_013396.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-06
Organism	Homo sapiens(Human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075792.1, AJ010598.1 and BM149594.1. On Sep 25, 2013 this sequence version replaced NM_013396.3. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (USP25a) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075792.1, AF134213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037528.3
CDS	370..3537
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_013396.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013396.4

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_013396.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_037528.3 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.

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CloneID	OHu12469
Clone ID Related Accession (Same CDS sequence)	NM_013396.4 , NM_013396.6 , NM_013396.5
Accession Version	NM_013396.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, BC075792.1, AJ010598.1 and BM149594.1. On Jun 2, 2019 this sequence version replaced NM_013396.5. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (USP25a) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075792.1, AF134213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037528.3
CDS	413..3580
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_013396.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013396.6

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP25 ( NM_013396.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_037528.3 Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.

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CloneID	OHu12469
Clone ID Related Accession (Same CDS sequence)	NM_013396.4 , NM_013396.6 , NM_013396.5
Accession Version	NM_013396.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 25 isoform USP25a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ010597.1, BC075792.1, AJ010598.1 and BM149594.1. On Jun 18, 2017 this sequence version replaced NM_013396.4. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (USP25a) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075792.1, AF134213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037528.3
CDS	461..3628
Translation	MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Target ORF information:

RefSeq Version	NM_013396.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 25 (USP25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013396.5

ORF Insert Sequence:

ATGACCGTGG AGCAGAACGT GCTGCAGCAG AGCGCGGCGC AGAAGCACCA GCAGACGTTT 
TTGAATCAAC TGAGAGAAAT TACGGGGATT AATGACACCC AGATACTACA GCAAGCCTTG 
AAGGATAGTA ATGGAAACTT GGAATTAGCA GTGGCTTTCC TTACTGCGAA GAATGCTAAG 
ACCCCTCAGC AGGAGGAGAC AACTTACTAC CAAACAGCAC TTCCTGGCAA TGATAGATAC 
ATCAGTGTGG GAAGCCAAGC AGATACAAAT GTGATTGATC TCACTGGAGA TGATAAAGAT 
GATCTTCAGA GAGCAATTGC CTTGAGTTTG GCCGAATCAA ACAGGGCATT CAGGGAGACT 
GGAATAACTG ATGAGGAACA AGCCATTAGC AGAGTTCTTG AAGCCAGCAT AGCAGAGAAT 
AAAGCATGTT TGAAGAGGAC ACCTACAGAA GTTTGGAGGG ATTCTCGAAA CCCTTATGAT 
AGAAAAAGAC AGGACAAAGC TCCCGTTGGG CTAAAGAATG TTGGCAATAC TTGTTGGTTT 
AGTGCTGTTA TTCAGTCATT ATTTAATCTT TTGGAATTTA GAAGATTAGT TCTGAATTAC 
AAGCCTCCAT CAAATGCTCA AGATTTACCC CGAAACCAAA AGGAACATCG GAATTTGCCT 
TTTATGCGTG AGCTGAGGTA TCTATTTGCA CTTCTTGTTG GTACCAAAAG GAAGTATGTT 
GATCCATCAA GAGCAGTTGA AATTCTTAAG GATGCTTTCA AATCAAATGA CTCACAGCAG 
CAAGATGTGA GTGAGTTTAC ACACAAATTA TTAGATTGGT TAGAAGATGC CTTCCAAATG 
AAAGCTGAAG AGGAGACGGA TGAAGAGAAG CCAAAGAACC CCATGGTAGA GTTGTTCTAT 
GGCAGATTCC TGGCTGTGGG AGTACTTGAA GGTAAAAAAT TTGAAAACAC TGAAATGTTT 
GGTCAGTACC CACTTCAGGT CAATGGGTTC AAAGATCTGC ATGAGTGCCT AGAAGCTGCA 
ATGATTGAAG GAGAAATTGA GTCTTTACAT TCAGAGAATT CAGGAAAATC AGGCCAAGAG 
CATTGGTTTA CTGAATTACC ACCTGTGTTA ACATTTGAAT TGTCAAGATT TGAATTTAAT 
CAGGCATTGG GAAGACCAGA AAAAATTCAC AACAAATTAG AATTTCCCCA AGTTTTATAT 
TTGGACAGAT ACATGCACAG AAACAGAGAA ATAACAAGAA TTAAGAGGGA AGAGATCAAG 
AGACTGAAAG ATTACCTCAC GGTATTACAA CAAAGGCTAG AAAGATATTT AAGCTATGGT 
TCCGGTCCCA AACGATTCCC CTTGGTAGAT GTTCTTCAGT ATGCATTGGA ATTTGCCTCA 
AGTAAACCTG TTTGCACTTC TCCTGTTGAC GATATTGACG CTAGTTCCCC ACCTAGTGGT 
TCCATACCAT CACAGACATT ACCAAGCACA ACAGAACAAC AGGGAGCCCT ATCTTCAGAA 
CTGCCAAGCA CATCACCTTC ATCAGTTGCT GCCATTTCAT CGAGATCAGT AATACACAAA 
CCATTTACTC AGTCCCGGAT ACCTCCAGAT TTGCCCATGC ATCCGGCACC AAGGCACATA 
ACGGAGGAAG AACTTTCTGT GCTGGAAAGT TGTTTACATC GCTGGAGGAC AGAAATAGAA 
AATGACACCA GAGATTTGCA GGAAAGCATA TCCAGAATCC ATCGAACAAT TGAATTAATG 
TACTCTGACA AATCTATGAT ACAAGTTCCT TATCGATTAC ATGCCGTTTT AGTTCACGAA 
GGCCAAGCTA ATGCTGGGCA CTACTGGGCA TATATTTTTG ATCATCGTGA AAGCAGATGG 
ATGAAGTACA ATGATATTGC TGTGACAAAA TCATCATGGG AAGAGCTAGT GAGGGACTCT 
TTTGGTGGTT ATAGAAATGC CAGTGCATAC TGTTTAATGT ACATAAATGA TAAGGCACAG 
TTCCTAATAC AAGAGGAGTT TAATAAAGAA ACTGGGCAGC CCCTTGTTGG TATAGAAACA 
TTACCACCGG ATTTGAGAGA TTTTGTTGAG GAAGACAACC AACGATTTGA AAAAGAACTA 
GAAGAATGGG ATGCACAACT TGCCCAGAAA GCTTTGCAGG AAAAGCTTTT AGCGTCTCAG 
AAATTGAGAG AGTCAGAGAC TTCTGTGACA ACAGCACAAG CAGCAGGAGA CCCAGAATAT 
CTAGAGCAGC CATCAAGAAG TGATTTCTCA AAGCACTTGA AAGAAGAAAC TATTCAAATA 
ATTACCAAGG CATCACATGA GCATGAAGAT AAAAGTCCTG AAACAGTTTT GCAGTCGGCA 
ATTAAGTTGG AATATGCAAG GTTGGTTAAG TTGGCCCAAG AAGACACCCC ACCAGAAACC 
GATTATCGTT TACATCATGT AGTGGTCTAC TTTATCCAGA ACCAGGCACC AAAGAAAATT 
ATTGAGAAAA CATTACTAGA ACAATTTGGA GATAGAAATT TGAGTTTTGA TGAAAGGTGT 
CACAACATAA TGAAAGTTGC TCAAGCCAAA CTGGAAATGA TAAAACCTGA AGAAGTAAAC 
TTGGAGGAAT ATGAGGAGTG GCATCAGGAT TATAGGAAAT TCAGGGAAAC AACTATGTAT 
CTCATAATTG GGCTAGAAAA TTTTCAAAGA GAAAGTTATA TAGATTCCTT GCTGTTCCTC 
ATCTGTGCTT ATCAGAATAA CAAAGAACTC TTGTCTAAAG GCTTATACAG AGGACATGAT 
GAAGAATTGA TATCACATTA TAGAAGAGAA TGTTTGCTAA AATTAAATGA GCAAGCCGCA 
GAACTCTTCG AATCTGGAGA GGATCGAGAA GTAAACAATG GTTTGATTAT CATGAATGAG 
TTTATTGTCC CATTTTTGCC ATTATTACTG GTGGATGAAA TGGAAGAAAA GGATATACTA 
GCTGTAGAAG ATATGAGAAA TCGATGGTGT TCCTACCTTG GTCAAGAAAT GGAACCACAC 
CTCCAAGAAA AGCTGACAGA TTTTTTGCCA AAACTGCTTG ATTGTTCTAT GGAGATTAAA 
AGTTTCCATG AGCCACCGAA GTTACCTTCA TATTCCACGC ATGAACTCTG TGAGCGATTT 
GCCCGAATCA TGTTGTCCCT CAGTCGAACT CCTGCTGATG GAAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	USP25
Entrez Gene ID	29761
Full Name	ubiquitin specific peptidase 25
Synonyms	USP21
General protein information	Preferred Names ubiquitin specific peptidase 25 Names ubiquitin carboxyl-terminal hydrolase 25 USP on chromosome 21 deubiquitinating enzyme 25 ubiquitin thioesterase 25 ubiquitin thiolesterase 25 ubiquitin-specific processing protease 25
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q21.1
Summary	Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008].
Disorder MIM:	604736

Mus musculus (house mouse)	Usp25	NP_038946.2
Rattus norvegicus (Norway rat)	Usp25	NP_001100584.1
Homo sapiens (human)	USP25	NP_001269970.1
	USP25	XP_001107973.2
Canis lupus familiaris (dog)	USP25	XP_535562.3
Xenopus tropicalis (tropical clawed frog)	usp25	NP_001039152.1
Bos taurus (cattle)	USP25	XP_005201215.1
Gallus gallus (chicken)	USP25	XP_004938410.1
Pan troglodytes (chimpanzee)	USP25	XP_531393.2
Danio rerio (zebrafish)	usp25	NP_001001886.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

USP25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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