col5a3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col5a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col5a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa57306 XM_031901022.1
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57307 XM_004913713.3
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57308 XM_031901023.1
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57309 XM_004913714.4
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57310 XM_031901024.1
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57311 XM_031901025.1
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57312 XM_031901026.1
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa09043 XM_004913713.2
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa09044 XM_004913714.3
Latest version!
Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa57306
    Clone ID Related Accession (Same CDS sequence) XM_031901022.1
    Accession Version XM_031901022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5625bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGTAA AGGCCAATGT GGTTGACTAC
    AATCAATACA GTGAAGGAGA CTATAGTTAT CAGACAGATA CTCCTGCCCT TACTACAGGA
    CCCAATGAGC AAAAACCAGA GGAGGAGTCT TTGGTGGAGG AATCCATTGT GGAGGAATCC
    ATTGTGGAGG ACCCATTTGT GGAGGAGTAC GTAACAGGAG AGGACTATGA ATCAGAGAAA
    AAGCTGGTTG AGGAAACAGA GTATGGGGTA AAAACTGTTA GCCCCACAGA GTCTGAGCCA
    GTAGTAGGAG AAGGTTTAGG AGAATATGAT ATTTATGACT ATAAGGAGTA TGAAGAGAAG
    CTCCCCAACC CTACCACACA AGAGGTCGGC CCTGGTGTGC CTGCTGAAAC TGATATCTCT
    GAAACCAGTG TGACCAATTA TGGTGGGTAT GGCGAGAAAG GCCAGAAAGG AGAGCCAGCA
    GTTATTGAGC CGGGAATGCT GATTGAAGGC CCCCCTGGTT TGGAGGGACC TGCTGGTCTT
    TCTGGTCCAC CTGGTTTGCA AGGACCCCCT GGTATTGCTG GAGATCCTGG TGAGAGAGGT
    CCACCTGGTC GCGCAGGGTT ACCGGGTGCT GATGGGTTAC CAGGGCCTCC AGGCACCATG
    CTGATGTTAC CGTTCCGATA TGGTGGTGAT GGAGAAAAAG GACCAACAAT CTCAGCCCAA
    GAAGCACAGG CTCAGGCCAT TCTTCAACAA GCTCGGATTG CCATGCGTGG ACCACCAGGC
    CCAATGGGAC TTACTGGAAG ATCTGGCCCT GTGGGAGCAC CTGGAGCTGC AGGAATTAAG
    GGAGACCCAG GAGACCCAGG CCCTCAGGGT CCTAGAGGAA TTCAGGGAAC ACTTGGATCC
    ATGGGAAAAT CTGGAAAAAG GGGACGCTCT GGAGCTGATG GAGCCCGTGG AATGCCAGGA
    GAGCCTGGGT CCAAGGGTGA CAGAGGTTTC GATGGTCTTC CAGGTCTCCC CGGGGAGAAG
    GGACACAGGG GTGACAAAGG TCCTCATGGG CCACCAGGGC CTCCTGGTGA AGATGGAGAG
    AGGGGAGAAG ATGGAGAAGT TGGACCAAGA GGGCTTCCCG GTGAACCTGG TGAAAGAGGA
    CTATTAGGAC CAAGGGGACC ACCAGGACCT TCAGGACAGC CTGGTGTTGC TGGTGTGGAT
    GGACCTCAAG GCCCCAAAGG AAACATGGGC CCTCAAGGAG AGCCTGGCCC TGCAGGTCAA
    CAAGGAATTC CAGGCCCCCA AGGTCTCCCT GGTCCACAAG GCTCCATCGG TCCACCTGGT
    GAGAAGGGTC CACAAGGAAA GCCTGGTTTG CCTGGATTAG CTGGAGCTGA CGGACCCCCT
    GGACATCCTG GTAAAGAAGG CCCCCCAGGA GAGAAGGGAG CATTGGGTCC CACTGGTCCC
    CAAGGTCCGA TTGGTTACCC TGGACCTCGC GGTGTTAAGG GAGCAGATGG TGTCCGAGGA
    TTGAAAGGAG GCAAAGGAGA AAAGGGTGAA GATGGATTTC CAGGATTTAA GGGTGACATG
    GGTCTTAAAG GAGACAGAGG AGAAGTTGGT CCTCTAGGTC CTCGGGGAGA AGATGGCCCA
    GAAGGTCCAA AGGGAAGATC TGGTCCATCA GGAGAATCTG GCCCAACTGG TCTTGCTGGT
    GAAAAGGGTA AACTTGGAGT CCCAGGATTG CCCGGATACC CAGGTCGACA GGGTCCCAAG
    GGTTCTACAG GTTTCCCTGG ATTTGCAGGA GCCAACGGCG AGAAAGGAGC TCGGGGTGTA
    GCTGGCAAAC CAGGTCCAAG GGGACAACGA GGTCCAACGG GTCCACGTGG TGCTAGAGGT
    CCAAGAGGTC CTACAGGAAA ACCAGGTCCT AAGGGCACAG CAGGCAATGA TGGTCCACCT
    GGCCCACCTG GTGAAAGAGG ACCTCAAGGA CCACAGGGCC CAGTTGGGTT CCCAGGACCG
    AAGGGCCCCC CTGGACCTCC TGGAAAGGAT GGATTGCCTG GCCACCCTGG ACAGCGAGGA
    GAAACAGGTT TCCAAGGAAA AACTGGGCCT CCTGGTCCTG GAGGTGTAGT AGGTCCACAG
    GGTCCCACCG GAGAAACTGG TCCCATTGGT GAAAGAGGGC ATCCTGGCCC ACCAGGTCCC
    CCTGGAGAGC AAGGTTTACC TGGATCAGCC GGAAAAGAGG GTGCTAAGGG AGATCCTGGT
    CCTTCAGGTG TGACTGGAAA AGATGGTCCC TCAGGTCTTC GTGGCTTCCC TGGTGAGAGA
    GGCCTACCTG GAGCTTCGGG TGCTGCAGGT TTGAAGGGTG GAGAAGGACC CCAGGGCCCA
    CCTGGTCCAC TTGGATCACC AGGTGAGAGA GGAGCTGCCG GCGCTGCTGG ACCAATTGGT
    CTGCCAGGAA GACCAGGACC TCAAGGCCCA CCAGGACCAG CAGGAGAGAA GGGGGCTCCA
    GGAGAGAAAG GACCTCAGGG ACCAGCAGGA CGTGATGGAA TACAAGGCCC AGTGGGACTT
    CCAGGACCTG CAGGTCCCCA AGGACCACCT GGGGAAGATG GTGACAAGGG TGAAATTGGA
    GAACCAGGAC AGAAAGGAGG AAAGGGTGAC AAAGGAGAAC AGGGTCCTCC TGGACCAGGA
    GGTCTACAAG GTCCTGTTGG AGCACCAGGT CCAGCGGGTT CTGATGGAGA GCCAGGTCCT
    AGAGGTCAAC AGGGAATGTT TGGGCAAAAG GGAGATGAAG GTTCTCGTGG ATTCCCAGGT
    CCACCTGGCC CAATTGGTCT CCAAGGACTT CCTGGACCCC CAGGTGAAAA GGGTGAAAAT
    GGAGATGTTG GTCCTATGGG TCCACCCGGT CCCCCTGGTC CTAGAGGTCC TCAAGGCCCT
    AATGGAGCTG ATGGTCCTCA AGGTCCTCCT GGATCTATTG GATCTGTTGG TCCAGTTGGT
    GAAAAGGGTG AACCAGGAGA AGCTGGAAAC CCAGGTCCCC CAGGAGAAGC TGGTGTTGGC
    GGTCCTAAAG GTGAAAGGGG AGAGAAAGGA GAGGCTGGAC CCCCAGGAGC TGCAGGACCT
    GCTGGTCCCA AGGGACCTCC AGGAGATGAT GGTCCTAAGG GAAATCCCGG TCCTGTTGGA
    TTCCCAGGAG ATCCTGGTCC ACCTGGAGAA CCTGGTCCAA GTGGACAAGA TGGTGTTTCT
    GGTGATAAAG GAGAGGATGG AGAATCAGGA CAGCCTGGTC CACCTGGTCC CTCTGGAGAA
    GCTGGACCAC CAGGACCTCC AGGAAAGAGG GGTCCTCCTG GTGCTAATGG TGCAGAAGGA
    AGACAAGGAG AGAAAGGTGC AAAGGGTGAG GCTGGAGCAG AAGGAGCACC TGGTAAGACT
    GGTCCTGTTG GACCACAAGG ACCTGCTGGG AAACCAGGAC CTGAAGGACT TAGAGGAATT
    CCAGGGCCTG TGGGAGAACA AGGACTTCCA GGTGCCCCTG GGCAGGATGG ACCTCCAGGT
    CCAATGGGTC CTCCCGGTTT ACCAGGTCTA AAAGGAGATC CCGGTACTAA GGGTGAGAAG
    GGACATCCAG GTCTAATTGG TCTGATTGGG CCACCAGGAG AGCAGGGTGA AAAGGGTGAT
    AGAGGACTTC CCGGTTTACA AGGTTCTCAC GGAACCAAAG GAGATATTGG TATATCAGGC
    CCTGCTGGTC CCACAGGTCC ACCTGGACCC CCTGGTTTAC CCGGTCCTCA AGGTCCGAAG
    GGTTCAAAGG GTTCATCTGG TCCAGCTGGT CAGAAGGGAG ATGGTGGTCT TCCTGGACCT
    CCTGGTCCAC CTGGCCCTCC TGGGGAAGTG ATCCAACCAT TACCTATCCA GTCACCAAAG
    AAGACCCGCA GATCTTCAGA TGATATGATG GCAGATGCTG GAGACAACAA CTTGGAGTAT
    GCAGATGGAA TGGAGGAGAT TTTTGGATCC CTCACTTCAT TAAAGCAAGA TATCGAGAGC
    ATGAAATTCC CCATAGGAAG TCAGAGCAAC CCAGCAAGGA CATGCAAAGA CCTGCAGCTC
    TGTCACCCAG AACTCCCTGA TGGTGAATAC TGGATTGATC CAAACCAGGG CTGCTCTGGT
    GATTCATTCA AGGTCTACTG CAATTTCACA GCTGGAGGAG AGACTTGTAT AAATCCAGAC
    AAGAAATCAG AAGGAGTGCG CATATCATCC TGGCCCAAAG AAAACCCTGG CTCATGGTTC
    AGTGAGTTCA AGAGAGGAAA GCTGCTTTCT TATGTGGACA TTCAGGGTAA CGCTATTAAC
    CAAGTACAAA TGACCTTCCT AAAACTCCTC AGTCAATCTG CCCGGCAAAA CTTCACCTAT
    ACTTGTCAAC AATCTGTTGC TTGGTATGAC AGTGTGGGCA AGCACTATGA CAAGGCCCTA
    AGGTTCCTTG GATCCAACGA CGAGGAAATT TCTTATGACA ACAGCCCATA CATTAGACCT
    TTGCATGATG GCTGTGCGCT CAAGAAAGGA TTTGCACGAA CAGTCCTTGA AATTAATACC
    CTGAAGATAG ATCAGCTGCC CATTGCAGAT GTAATGATCA GTGACTTTGG GGATCAGAAC
    CAGAAATTTG GATTTGAGGT CGGACCGGTC TGCTTCTTAG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756882.1
    CDS300..5924
    Translation

    Target ORF information:

    RefSeq Version XM_031901022.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGTAA AGGCCAATGT GGTTGACTAC
    AATCAATACA GTGAAGGAGA CTATAGTTAT CAGACAGATA CTCCTGCCCT TACTACAGGA
    CCCAATGAGC AAAAACCAGA GGAGGAGTCT TTGGTGGAGG AATCCATTGT GGAGGAATCC
    ATTGTGGAGG ACCCATTTGT GGAGGAGTAC GTAACAGGAG AGGACTATGA ATCAGAGAAA
    AAGCTGGTTG AGGAAACAGA GTATGGGGTA AAAACTGTTA GCCCCACAGA GTCTGAGCCA
    GTAGTAGGAG AAGGTTTAGG AGAATATGAT ATTTATGACT ATAAGGAGTA TGAAGAGAAG
    CTCCCCAACC CTACCACACA AGAGGTCGGC CCTGGTGTGC CTGCTGAAAC TGATATCTCT
    GAAACCAGTG TGACCAATTA TGGTGGGTAT GGCGAGAAAG GCCAGAAAGG AGAGCCAGCA
    GTTATTGAGC CGGGAATGCT GATTGAAGGC CCCCCTGGTT TGGAGGGACC TGCTGGTCTT
    TCTGGTCCAC CTGGTTTGCA AGGACCCCCT GGTATTGCTG GAGATCCTGG TGAGAGAGGT
    CCACCTGGTC GCGCAGGGTT ACCGGGTGCT GATGGGTTAC CAGGGCCTCC AGGCACCATG
    CTGATGTTAC CGTTCCGATA TGGTGGTGAT GGAGAAAAAG GACCAACAAT CTCAGCCCAA
    GAAGCACAGG CTCAGGCCAT TCTTCAACAA GCTCGGATTG CCATGCGTGG ACCACCAGGC
    CCAATGGGAC TTACTGGAAG ATCTGGCCCT GTGGGAGCAC CTGGAGCTGC AGGAATTAAG
    GGAGACCCAG GAGACCCAGG CCCTCAGGGT CCTAGAGGAA TTCAGGGAAC ACTTGGATCC
    ATGGGAAAAT CTGGAAAAAG GGGACGCTCT GGAGCTGATG GAGCCCGTGG AATGCCAGGA
    GAGCCTGGGT CCAAGGGTGA CAGAGGTTTC GATGGTCTTC CAGGTCTCCC CGGGGAGAAG
    GGACACAGGG GTGACAAAGG TCCTCATGGG CCACCAGGGC CTCCTGGTGA AGATGGAGAG
    AGGGGAGAAG ATGGAGAAGT TGGACCAAGA GGGCTTCCCG GTGAACCTGG TGAAAGAGGA
    CTATTAGGAC CAAGGGGACC ACCAGGACCT TCAGGACAGC CTGGTGTTGC TGGTGTGGAT
    GGACCTCAAG GCCCCAAAGG AAACATGGGC CCTCAAGGAG AGCCTGGCCC TGCAGGTCAA
    CAAGGAATTC CAGGCCCCCA AGGTCTCCCT GGTCCACAAG GCTCCATCGG TCCACCTGGT
    GAGAAGGGTC CACAAGGAAA GCCTGGTTTG CCTGGATTAG CTGGAGCTGA CGGACCCCCT
    GGACATCCTG GTAAAGAAGG CCCCCCAGGA GAGAAGGGAG CATTGGGTCC CACTGGTCCC
    CAAGGTCCGA TTGGTTACCC TGGACCTCGC GGTGTTAAGG GAGCAGATGG TGTCCGAGGA
    TTGAAAGGAG GCAAAGGAGA AAAGGGTGAA GATGGATTTC CAGGATTTAA GGGTGACATG
    GGTCTTAAAG GAGACAGAGG AGAAGTTGGT CCTCTAGGTC CTCGGGGAGA AGATGGCCCA
    GAAGGTCCAA AGGGAAGATC TGGTCCATCA GGAGAATCTG GCCCAACTGG TCTTGCTGGT
    GAAAAGGGTA AACTTGGAGT CCCAGGATTG CCCGGATACC CAGGTCGACA GGGTCCCAAG
    GGTTCTACAG GTTTCCCTGG ATTTGCAGGA GCCAACGGCG AGAAAGGAGC TCGGGGTGTA
    GCTGGCAAAC CAGGTCCAAG GGGACAACGA GGTCCAACGG GTCCACGTGG TGCTAGAGGT
    CCAAGAGGTC CTACAGGAAA ACCAGGTCCT AAGGGCACAG CAGGCAATGA TGGTCCACCT
    GGCCCACCTG GTGAAAGAGG ACCTCAAGGA CCACAGGGCC CAGTTGGGTT CCCAGGACCG
    AAGGGCCCCC CTGGACCTCC TGGAAAGGAT GGATTGCCTG GCCACCCTGG ACAGCGAGGA
    GAAACAGGTT TCCAAGGAAA AACTGGGCCT CCTGGTCCTG GAGGTGTAGT AGGTCCACAG
    GGTCCCACCG GAGAAACTGG TCCCATTGGT GAAAGAGGGC ATCCTGGCCC ACCAGGTCCC
    CCTGGAGAGC AAGGTTTACC TGGATCAGCC GGAAAAGAGG GTGCTAAGGG AGATCCTGGT
    CCTTCAGGTG TGACTGGAAA AGATGGTCCC TCAGGTCTTC GTGGCTTCCC TGGTGAGAGA
    GGCCTACCTG GAGCTTCGGG TGCTGCAGGT TTGAAGGGTG GAGAAGGACC CCAGGGCCCA
    CCTGGTCCAC TTGGATCACC AGGTGAGAGA GGAGCTGCCG GCGCTGCTGG ACCAATTGGT
    CTGCCAGGAA GACCAGGACC TCAAGGCCCA CCAGGACCAG CAGGAGAGAA GGGGGCTCCA
    GGAGAGAAAG GACCTCAGGG ACCAGCAGGA CGTGATGGAA TACAAGGCCC AGTGGGACTT
    CCAGGACCTG CAGGTCCCCA AGGACCACCT GGGGAAGATG GTGACAAGGG TGAAATTGGA
    GAACCAGGAC AGAAAGGAGG AAAGGGTGAC AAAGGAGAAC AGGGTCCTCC TGGACCAGGA
    GGTCTACAAG GTCCTGTTGG AGCACCAGGT CCAGCGGGTT CTGATGGAGA GCCAGGTCCT
    AGAGGTCAAC AGGGAATGTT TGGGCAAAAG GGAGATGAAG GTTCTCGTGG ATTCCCAGGT
    CCACCTGGCC CAATTGGTCT CCAAGGACTT CCTGGACCCC CAGGTGAAAA GGGTGAAAAT
    GGAGATGTTG GTCCTATGGG TCCACCCGGT CCCCCTGGTC CTAGAGGTCC TCAAGGCCCT
    AATGGAGCTG ATGGTCCTCA AGGTCCTCCT GGATCTATTG GATCTGTTGG TCCAGTTGGT
    GAAAAGGGTG AACCAGGAGA AGCTGGAAAC CCAGGTCCCC CAGGAGAAGC TGGTGTTGGC
    GGTCCTAAAG GTGAAAGGGG AGAGAAAGGA GAGGCTGGAC CCCCAGGAGC TGCAGGACCT
    GCTGGTCCCA AGGGACCTCC AGGAGATGAT GGTCCTAAGG GAAATCCCGG TCCTGTTGGA
    TTCCCAGGAG ATCCTGGTCC ACCTGGAGAA CCTGGTCCAA GTGGACAAGA TGGTGTTTCT
    GGTGATAAAG GAGAGGATGG AGAATCAGGA CAGCCTGGTC CACCTGGTCC CTCTGGAGAA
    GCTGGACCAC CAGGACCTCC AGGAAAGAGG GGTCCTCCTG GTGCTAATGG TGCAGAAGGA
    AGACAAGGAG AGAAAGGTGC AAAGGGTGAG GCTGGAGCAG AAGGAGCACC TGGTAAGACT
    GGTCCTGTTG GACCACAAGG ACCTGCTGGG AAACCAGGAC CTGAAGGACT TAGAGGAATT
    CCAGGGCCTG TGGGAGAACA AGGACTTCCA GGTGCCCCTG GGCAGGATGG ACCTCCAGGT
    CCAATGGGTC CTCCCGGTTT ACCAGGTCTA AAAGGAGATC CCGGTACTAA GGGTGAGAAG
    GGACATCCAG GTCTAATTGG TCTGATTGGG CCACCAGGAG AGCAGGGTGA AAAGGGTGAT
    AGAGGACTTC CCGGTTTACA AGGTTCTCAC GGAACCAAAG GAGATATTGG TATATCAGGC
    CCTGCTGGTC CCACAGGTCC ACCTGGACCC CCTGGTTTAC CCGGTCCTCA AGGTCCGAAG
    GGTTCAAAGG GTTCATCTGG TCCAGCTGGT CAGAAGGGAG ATGGTGGTCT TCCTGGACCT
    CCTGGTCCAC CTGGCCCTCC TGGGGAAGTG ATCCAACCAT TACCTATCCA GTCACCAAAG
    AAGACCCGCA GATCTTCAGA TGATATGATG GCAGATGCTG GAGACAACAA CTTGGAGTAT
    GCAGATGGAA TGGAGGAGAT TTTTGGATCC CTCACTTCAT TAAAGCAAGA TATCGAGAGC
    ATGAAATTCC CCATAGGAAG TCAGAGCAAC CCAGCAAGGA CATGCAAAGA CCTGCAGCTC
    TGTCACCCAG AACTCCCTGA TGGTGAATAC TGGATTGATC CAAACCAGGG CTGCTCTGGT
    GATTCATTCA AGGTCTACTG CAATTTCACA GCTGGAGGAG AGACTTGTAT AAATCCAGAC
    AAGAAATCAG AAGGAGTGCG CATATCATCC TGGCCCAAAG AAAACCCTGG CTCATGGTTC
    AGTGAGTTCA AGAGAGGAAA GCTGCTTTCT TATGTGGACA TTCAGGGTAA CGCTATTAAC
    CAAGTACAAA TGACCTTCCT AAAACTCCTC AGTCAATCTG CCCGGCAAAA CTTCACCTAT
    ACTTGTCAAC AATCTGTTGC TTGGTATGAC AGTGTGGGCA AGCACTATGA CAAGGCCCTA
    AGGTTCCTTG GATCCAACGA CGAGGAAATT TCTTATGACA ACAGCCCATA CATTAGACCT
    TTGCATGATG GCTGTGCGCT CAAGAAAGGA TTTGCACGAA CAGTCCTTGA AATTAATACC
    CTGAAGATAG ATCAGCTGCC CATTGCAGAT GTAATGATCA GTGACTTTGG GGATCAGAAC
    CAGAAATTTG GATTTGAGGT CGGACCGGTC TGCTTCTTAG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57307
    Clone ID Related Accession (Same CDS sequence) XM_004913713.3
    Accession Version XM_004913713.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGCCA ATGTGGTTGA CTACAATCAA
    TACAGTGAAG GAGACTATAG TTATCAGACA GATACTCCTG CCCTTACTAC AGGACCCAAT
    GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG GAGGAATCCA TTGTGGAGGA ATCCATTGTG
    GAGGACCCAT TTGTGGAGGA GTACGTAACA GGAGAGGACT ATGAATCAGA GAAAAAGCTG
    GTTGAGGAAA CAGAGTATGG GGTAAAAACT GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA
    GGAGAAGGTT TAGGAGAATA TGATATTTAT GACTATAAGG AGTATGAAGA GAAGCTCCCC
    AACCCTACCA CACAAGAGGT CGGCCCTGGT GTGCCTGCTG AAACTGATAT CTCTGAAACC
    AGTGTGACCA ATTATGGTGG GTATGGCGAG AAAGGCCAGA AAGGAGAGCC AGCAGTTATT
    GAGCCGGGAA TGCTGATTGA AGGCCCCCCT GGTTTGGAGG GACCTGCTGG TCTTTCTGGT
    CCACCTGGTT TGCAAGGACC CCCTGGTATT GCTGGAGATC CTGGTGAGAG AGGTCCACCT
    GGTCGCGCAG GGTTACCGGG TGCTGATGGG TTACCAGGGC CTCCAGGCAC CATGCTGATG
    TTACCGTTCC GATATGGTGG TGATGGAGAA AAAGGACCAA CAATCTCAGC CCAAGAAGCA
    CAGGCTCAGG CCATTCTTCA ACAAGCTCGG ATTGCCATGC GTGGACCACC AGGCCCAATG
    GGACTTACTG GAAGATCTGG CCCTGTGGGA GCACCTGGAG CTGCAGGAAT TAAGGGAGAC
    CCAGGAGACC CAGGCCCTCA GGGTCCTAGA GGAATTCAGG GAACACTTGG ATCCATGGGA
    AAATCTGGAA AAAGGGGACG CTCTGGAGCT GATGGAGCCC GTGGAATGCC AGGAGAGCCT
    GGGTCCAAGG GTGACAGAGG TTTCGATGGT CTTCCAGGTC TCCCCGGGGA GAAGGGACAC
    AGGGGTGACA AAGGTCCTCA TGGGCCACCA GGGCCTCCTG GTGAAGATGG AGAGAGGGGA
    GAAGATGGAG AAGTTGGACC AAGAGGGCTT CCCGGTGAAC CTGGTGAAAG AGGACTATTA
    GGACCAAGGG GACCACCAGG ACCTTCAGGA CAGCCTGGTG TTGCTGGTGT GGATGGACCT
    CAAGGCCCCA AAGGAAACAT GGGCCCTCAA GGAGAGCCTG GCCCTGCAGG TCAACAAGGA
    ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA CAAGGCTCCA TCGGTCCACC TGGTGAGAAG
    GGTCCACAAG GAAAGCCTGG TTTGCCTGGA TTAGCTGGAG CTGACGGACC CCCTGGACAT
    CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG GGAGCATTGG GTCCCACTGG TCCCCAAGGT
    CCGATTGGTT ACCCTGGACC TCGCGGTGTT AAGGGAGCAG ATGGTGTCCG AGGATTGAAA
    GGAGGCAAAG GAGAAAAGGG TGAAGATGGA TTTCCAGGAT TTAAGGGTGA CATGGGTCTT
    AAAGGAGACA GAGGAGAAGT TGGTCCTCTA GGTCCTCGGG GAGAAGATGG CCCAGAAGGT
    CCAAAGGGAA GATCTGGTCC ATCAGGAGAA TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG
    GGTAAACTTG GAGTCCCAGG ATTGCCCGGA TACCCAGGTC GACAGGGTCC CAAGGGTTCT
    ACAGGTTTCC CTGGATTTGC AGGAGCCAAC GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC
    AAACCAGGTC CAAGGGGACA ACGAGGTCCA ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA
    GGTCCTACAG GAAAACCAGG TCCTAAGGGC ACAGCAGGCA ATGATGGTCC ACCTGGCCCA
    CCTGGTGAAA GAGGACCTCA AGGACCACAG GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC
    CCCCCTGGAC CTCCTGGAAA GGATGGATTG CCTGGCCACC CTGGACAGCG AGGAGAAACA
    GGTTTCCAAG GAAAAACTGG GCCTCCTGGT CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC
    ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA GGGCATCCTG GCCCACCAGG TCCCCCTGGA
    GAGCAAGGTT TACCTGGATC AGCCGGAAAA GAGGGTGCTA AGGGAGATCC TGGTCCTTCA
    GGTGTGACTG GAAAAGATGG TCCCTCAGGT CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA
    CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG GGTGGAGAAG GACCCCAGGG CCCACCTGGT
    CCACTTGGAT CACCAGGTGA GAGAGGAGCT GCCGGCGCTG CTGGACCAAT TGGTCTGCCA
    GGAAGACCAG GACCTCAAGG CCCACCAGGA CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG
    AAAGGACCTC AGGGACCAGC AGGACGTGAT GGAATACAAG GCCCAGTGGG ACTTCCAGGA
    CCTGCAGGTC CCCAAGGACC ACCTGGGGAA GATGGTGACA AGGGTGAAAT TGGAGAACCA
    GGACAGAAAG GAGGAAAGGG TGACAAAGGA GAACAGGGTC CTCCTGGACC AGGAGGTCTA
    CAAGGTCCTG TTGGAGCACC AGGTCCAGCG GGTTCTGATG GAGAGCCAGG TCCTAGAGGT
    CAACAGGGAA TGTTTGGGCA AAAGGGAGAT GAAGGTTCTC GTGGATTCCC AGGTCCACCT
    GGCCCAATTG GTCTCCAAGG ACTTCCTGGA CCCCCAGGTG AAAAGGGTGA AAATGGAGAT
    GTTGGTCCTA TGGGTCCACC CGGTCCCCCT GGTCCTAGAG GTCCTCAAGG CCCTAATGGA
    GCTGATGGTC CTCAAGGTCC TCCTGGATCT ATTGGATCTG TTGGTCCAGT TGGTGAAAAG
    GGTGAACCAG GAGAAGCTGG AAACCCAGGT CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT
    AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT GGACCCCCAG GAGCTGCAGG ACCTGCTGGT
    CCCAAGGGAC CTCCAGGAGA TGATGGTCCT AAGGGAAATC CCGGTCCTGT TGGATTCCCA
    GGAGATCCTG GTCCACCTGG AGAACCTGGT CCAAGTGGAC AAGATGGTGT TTCTGGTGAT
    AAAGGAGAGG ATGGAGAATC AGGACAGCCT GGTCCACCTG GTCCCTCTGG AGAAGCTGGA
    CCACCAGGAC CTCCAGGAAA GAGGGGTCCT CCTGGTGCTA ATGGTGCAGA AGGAAGACAA
    GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA GCAGAAGGAG CACCTGGTAA GACTGGTCCT
    GTTGGACCAC AAGGACCTGC TGGGAAACCA GGACCTGAAG GACTTAGAGG AATTCCAGGG
    CCTGTGGGAG AACAAGGACT TCCAGGTGCC CCTGGGCAGG ATGGACCTCC AGGTCCAATG
    GGTCCTCCCG GTTTACCAGG TCTAAAAGGA GATCCCGGTA CTAAGGGTGA GAAGGGACAT
    CCAGGTCTAA TTGGTCTGAT TGGGCCACCA GGAGAGCAGG GTGAAAAGGG TGATAGAGGA
    CTTCCCGGTT TACAAGGTTC TCACGGAACC AAAGGAGATA TTGGTATATC AGGCCCTGCT
    GGTCCCACAG GTCCACCTGG ACCCCCTGGT TTACCCGGTC CTCAAGGTCC GAAGGGTTCA
    AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG GGAGATGGTG GTCTTCCTGG ACCTCCTGGT
    CCACCTGGCC CTCCTGGGGA AGTGATCCAA CCATTACCTA TCCAGTCACC AAAGAAGACC
    CGCAGATCTT CAGATGATAT GATGGCAGAT GCTGGAGACA ACAACTTGGA GTATGCAGAT
    GGAATGGAGG AGATTTTTGG ATCCCTCACT TCATTAAAGC AAGATATCGA GAGCATGAAA
    TTCCCCATAG GAAGTCAGAG CAACCCAGCA AGGACATGCA AAGACCTGCA GCTCTGTCAC
    CCAGAACTCC CTGATGGTGA ATACTGGATT GATCCAAACC AGGGCTGCTC TGGTGATTCA
    TTCAAGGTCT ACTGCAATTT CACAGCTGGA GGAGAGACTT GTATAAATCC AGACAAGAAA
    TCAGAAGGAG TGCGCATATC ATCCTGGCCC AAAGAAAACC CTGGCTCATG GTTCAGTGAG
    TTCAAGAGAG GAAAGCTGCT TTCTTATGTG GACATTCAGG GTAACGCTAT TAACCAAGTA
    CAAATGACCT TCCTAAAACT CCTCAGTCAA TCTGCCCGGC AAAACTTCAC CTATACTTGT
    CAACAATCTG TTGCTTGGTA TGACAGTGTG GGCAAGCACT ATGACAAGGC CCTAAGGTTC
    CTTGGATCCA ACGACGAGGA AATTTCTTAT GACAACAGCC CATACATTAG ACCTTTGCAT
    GATGGCTGTG CGCTCAAGAA AGGATTTGCA CGAACAGTCC TTGAAATTAA TACCCTGAAG
    ATAGATCAGC TGCCCATTGC AGATGTAATG ATCAGTGACT TTGGGGATCA GAACCAGAAA
    TTTGGATTTG AGGTCGGACC GGTCTGCTTC TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913770.1
    CDS300..5918
    Translation

    Target ORF information:

    RefSeq Version XM_004913713.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGCCA ATGTGGTTGA CTACAATCAA
    TACAGTGAAG GAGACTATAG TTATCAGACA GATACTCCTG CCCTTACTAC AGGACCCAAT
    GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG GAGGAATCCA TTGTGGAGGA ATCCATTGTG
    GAGGACCCAT TTGTGGAGGA GTACGTAACA GGAGAGGACT ATGAATCAGA GAAAAAGCTG
    GTTGAGGAAA CAGAGTATGG GGTAAAAACT GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA
    GGAGAAGGTT TAGGAGAATA TGATATTTAT GACTATAAGG AGTATGAAGA GAAGCTCCCC
    AACCCTACCA CACAAGAGGT CGGCCCTGGT GTGCCTGCTG AAACTGATAT CTCTGAAACC
    AGTGTGACCA ATTATGGTGG GTATGGCGAG AAAGGCCAGA AAGGAGAGCC AGCAGTTATT
    GAGCCGGGAA TGCTGATTGA AGGCCCCCCT GGTTTGGAGG GACCTGCTGG TCTTTCTGGT
    CCACCTGGTT TGCAAGGACC CCCTGGTATT GCTGGAGATC CTGGTGAGAG AGGTCCACCT
    GGTCGCGCAG GGTTACCGGG TGCTGATGGG TTACCAGGGC CTCCAGGCAC CATGCTGATG
    TTACCGTTCC GATATGGTGG TGATGGAGAA AAAGGACCAA CAATCTCAGC CCAAGAAGCA
    CAGGCTCAGG CCATTCTTCA ACAAGCTCGG ATTGCCATGC GTGGACCACC AGGCCCAATG
    GGACTTACTG GAAGATCTGG CCCTGTGGGA GCACCTGGAG CTGCAGGAAT TAAGGGAGAC
    CCAGGAGACC CAGGCCCTCA GGGTCCTAGA GGAATTCAGG GAACACTTGG ATCCATGGGA
    AAATCTGGAA AAAGGGGACG CTCTGGAGCT GATGGAGCCC GTGGAATGCC AGGAGAGCCT
    GGGTCCAAGG GTGACAGAGG TTTCGATGGT CTTCCAGGTC TCCCCGGGGA GAAGGGACAC
    AGGGGTGACA AAGGTCCTCA TGGGCCACCA GGGCCTCCTG GTGAAGATGG AGAGAGGGGA
    GAAGATGGAG AAGTTGGACC AAGAGGGCTT CCCGGTGAAC CTGGTGAAAG AGGACTATTA
    GGACCAAGGG GACCACCAGG ACCTTCAGGA CAGCCTGGTG TTGCTGGTGT GGATGGACCT
    CAAGGCCCCA AAGGAAACAT GGGCCCTCAA GGAGAGCCTG GCCCTGCAGG TCAACAAGGA
    ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA CAAGGCTCCA TCGGTCCACC TGGTGAGAAG
    GGTCCACAAG GAAAGCCTGG TTTGCCTGGA TTAGCTGGAG CTGACGGACC CCCTGGACAT
    CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG GGAGCATTGG GTCCCACTGG TCCCCAAGGT
    CCGATTGGTT ACCCTGGACC TCGCGGTGTT AAGGGAGCAG ATGGTGTCCG AGGATTGAAA
    GGAGGCAAAG GAGAAAAGGG TGAAGATGGA TTTCCAGGAT TTAAGGGTGA CATGGGTCTT
    AAAGGAGACA GAGGAGAAGT TGGTCCTCTA GGTCCTCGGG GAGAAGATGG CCCAGAAGGT
    CCAAAGGGAA GATCTGGTCC ATCAGGAGAA TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG
    GGTAAACTTG GAGTCCCAGG ATTGCCCGGA TACCCAGGTC GACAGGGTCC CAAGGGTTCT
    ACAGGTTTCC CTGGATTTGC AGGAGCCAAC GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC
    AAACCAGGTC CAAGGGGACA ACGAGGTCCA ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA
    GGTCCTACAG GAAAACCAGG TCCTAAGGGC ACAGCAGGCA ATGATGGTCC ACCTGGCCCA
    CCTGGTGAAA GAGGACCTCA AGGACCACAG GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC
    CCCCCTGGAC CTCCTGGAAA GGATGGATTG CCTGGCCACC CTGGACAGCG AGGAGAAACA
    GGTTTCCAAG GAAAAACTGG GCCTCCTGGT CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC
    ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA GGGCATCCTG GCCCACCAGG TCCCCCTGGA
    GAGCAAGGTT TACCTGGATC AGCCGGAAAA GAGGGTGCTA AGGGAGATCC TGGTCCTTCA
    GGTGTGACTG GAAAAGATGG TCCCTCAGGT CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA
    CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG GGTGGAGAAG GACCCCAGGG CCCACCTGGT
    CCACTTGGAT CACCAGGTGA GAGAGGAGCT GCCGGCGCTG CTGGACCAAT TGGTCTGCCA
    GGAAGACCAG GACCTCAAGG CCCACCAGGA CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG
    AAAGGACCTC AGGGACCAGC AGGACGTGAT GGAATACAAG GCCCAGTGGG ACTTCCAGGA
    CCTGCAGGTC CCCAAGGACC ACCTGGGGAA GATGGTGACA AGGGTGAAAT TGGAGAACCA
    GGACAGAAAG GAGGAAAGGG TGACAAAGGA GAACAGGGTC CTCCTGGACC AGGAGGTCTA
    CAAGGTCCTG TTGGAGCACC AGGTCCAGCG GGTTCTGATG GAGAGCCAGG TCCTAGAGGT
    CAACAGGGAA TGTTTGGGCA AAAGGGAGAT GAAGGTTCTC GTGGATTCCC AGGTCCACCT
    GGCCCAATTG GTCTCCAAGG ACTTCCTGGA CCCCCAGGTG AAAAGGGTGA AAATGGAGAT
    GTTGGTCCTA TGGGTCCACC CGGTCCCCCT GGTCCTAGAG GTCCTCAAGG CCCTAATGGA
    GCTGATGGTC CTCAAGGTCC TCCTGGATCT ATTGGATCTG TTGGTCCAGT TGGTGAAAAG
    GGTGAACCAG GAGAAGCTGG AAACCCAGGT CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT
    AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT GGACCCCCAG GAGCTGCAGG ACCTGCTGGT
    CCCAAGGGAC CTCCAGGAGA TGATGGTCCT AAGGGAAATC CCGGTCCTGT TGGATTCCCA
    GGAGATCCTG GTCCACCTGG AGAACCTGGT CCAAGTGGAC AAGATGGTGT TTCTGGTGAT
    AAAGGAGAGG ATGGAGAATC AGGACAGCCT GGTCCACCTG GTCCCTCTGG AGAAGCTGGA
    CCACCAGGAC CTCCAGGAAA GAGGGGTCCT CCTGGTGCTA ATGGTGCAGA AGGAAGACAA
    GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA GCAGAAGGAG CACCTGGTAA GACTGGTCCT
    GTTGGACCAC AAGGACCTGC TGGGAAACCA GGACCTGAAG GACTTAGAGG AATTCCAGGG
    CCTGTGGGAG AACAAGGACT TCCAGGTGCC CCTGGGCAGG ATGGACCTCC AGGTCCAATG
    GGTCCTCCCG GTTTACCAGG TCTAAAAGGA GATCCCGGTA CTAAGGGTGA GAAGGGACAT
    CCAGGTCTAA TTGGTCTGAT TGGGCCACCA GGAGAGCAGG GTGAAAAGGG TGATAGAGGA
    CTTCCCGGTT TACAAGGTTC TCACGGAACC AAAGGAGATA TTGGTATATC AGGCCCTGCT
    GGTCCCACAG GTCCACCTGG ACCCCCTGGT TTACCCGGTC CTCAAGGTCC GAAGGGTTCA
    AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG GGAGATGGTG GTCTTCCTGG ACCTCCTGGT
    CCACCTGGCC CTCCTGGGGA AGTGATCCAA CCATTACCTA TCCAGTCACC AAAGAAGACC
    CGCAGATCTT CAGATGATAT GATGGCAGAT GCTGGAGACA ACAACTTGGA GTATGCAGAT
    GGAATGGAGG AGATTTTTGG ATCCCTCACT TCATTAAAGC AAGATATCGA GAGCATGAAA
    TTCCCCATAG GAAGTCAGAG CAACCCAGCA AGGACATGCA AAGACCTGCA GCTCTGTCAC
    CCAGAACTCC CTGATGGTGA ATACTGGATT GATCCAAACC AGGGCTGCTC TGGTGATTCA
    TTCAAGGTCT ACTGCAATTT CACAGCTGGA GGAGAGACTT GTATAAATCC AGACAAGAAA
    TCAGAAGGAG TGCGCATATC ATCCTGGCCC AAAGAAAACC CTGGCTCATG GTTCAGTGAG
    TTCAAGAGAG GAAAGCTGCT TTCTTATGTG GACATTCAGG GTAACGCTAT TAACCAAGTA
    CAAATGACCT TCCTAAAACT CCTCAGTCAA TCTGCCCGGC AAAACTTCAC CTATACTTGT
    CAACAATCTG TTGCTTGGTA TGACAGTGTG GGCAAGCACT ATGACAAGGC CCTAAGGTTC
    CTTGGATCCA ACGACGAGGA AATTTCTTAT GACAACAGCC CATACATTAG ACCTTTGCAT
    GATGGCTGTG CGCTCAAGAA AGGATTTGCA CGAACAGTCC TTGAAATTAA TACCCTGAAG
    ATAGATCAGC TGCCCATTGC AGATGTAATG ATCAGTGACT TTGGGGATCA GAACCAGAAA
    TTTGGATTTG AGGTCGGACC GGTCTGCTTC TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57308
    Clone ID Related Accession (Same CDS sequence) XM_031901023.1
    Accession Version XM_031901023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGA AGAAGAAAGC CAGTTCCAAA TCGAAGAAGA AGCCAGTGCC CCCTAAATCA
    AAGGACAAAC CCCAAAAGGT CACGCCCAAA AACTCATTTG CAGCTAAGAA GAAGAAGGGC
    AAGACCGATT ACCAGGCGTC AGCAAGAAAA AGAATAGGGG TAAAGGCCAA TGTGGTTGAC
    TACAATCAAT ACAGTGAAGG AGACTATAGT TATCAGACAG ATACTCCTGC CCTTACTACA
    GGACCCAATG AGCAAAAACC AGAGGAGGAG TCTTTGGTGG AGGAATCCAT TGTGGAGGAA
    TCCATTGTGG AGGACCCATT TGTGGAGGAG TACGTAACAG GAGAGGACTA TGAATCAGAG
    AAAAAGCTGG TTGAGGAAAC AGAGTATGGG GTAAAAACTG TTAGCCCCAC AGAGTCTGAG
    CCAGTAGTAG GAGAAGGTTT AGGAGAATAT GATATTTATG ACTATAAGGA GTATGAAGAG
    AAGCTCCCCA ACCCTACCAC ACAAGAGGTC GGCCCTGGTG TGCCTGCTGA AACTGATATC
    TCTGAAACCA GTGTGACCAA TTATGGTGGG TATGGCGAGA AAGGCCAGAA AGGAGAGCCA
    GCAGTTATTG AGCCGGGAAT GCTGATTGAA GGCCCCCCTG GTTTGGAGGG ACCTGCTGGT
    CTTTCTGGTC CACCTGGTTT GCAAGGACCC CCTGGTATTG CTGGAGATCC TGGTGAGAGA
    GGTCCACCTG GTCGCGCAGG GTTACCGGGT GCTGATGGGT TACCAGGGCC TCCAGGCACC
    ATGCTGATGT TACCGTTCCG ATATGGTGGT GATGGAGAAA AAGGACCAAC AATCTCAGCC
    CAAGAAGCAC AGGCTCAGGC CATTCTTCAA CAAGCTCGGA TTGCCATGCG TGGACCACCA
    GGCCCAATGG GACTTACTGG AAGATCTGGC CCTGTGGGAG CACCTGGAGC TGCAGGAATT
    AAGGGAGACC CAGGAGACCC AGGCCCTCAG GGTCCTAGAG GAATTCAGGG AACACTTGGA
    TCCATGGGAA AATCTGGAAA AAGGGGACGC TCTGGAGCTG ATGGAGCCCG TGGAATGCCA
    GGAGAGCCTG GGTCCAAGGG TGACAGAGGT TTCGATGGTC TTCCAGGTCT CCCCGGGGAG
    AAGGGACACA GGGGTGACAA AGGTCCTCAT GGGCCACCAG GGCCTCCTGG TGAAGATGGA
    GAGAGGGGAG AAGATGGAGA AGTTGGACCA AGAGGGCTTC CCGGTGAACC TGGTGAAAGA
    GGACTATTAG GACCAAGGGG ACCACCAGGA CCTTCAGGAC AGCCTGGTGT TGCTGGTGTG
    GATGGACCTC AAGGCCCCAA AGGAAACATG GGCCCTCAAG GAGAGCCTGG CCCTGCAGGT
    CAACAAGGAA TTCCAGGCCC CCAAGGTCTC CCTGGTCCAC AAGGCTCCAT CGGTCCACCT
    GGTGAGAAGG GTCCACAAGG AAAGCCTGGT TTGCCTGGAT TAGCTGGAGC TGACGGACCC
    CCTGGACATC CTGGTAAAGA AGGCCCCCCA GGAGAGAAGG GAGCATTGGG TCCCACTGGT
    CCCCAAGGTC CGATTGGTTA CCCTGGACCT CGCGGTGTTA AGGGAGCAGA TGGTGTCCGA
    GGATTGAAAG GAGGCAAAGG AGAAAAGGGT GAAGATGGAT TTCCAGGATT TAAGGGTGAC
    ATGGGTCTTA AAGGAGACAG AGGAGAAGTT GGTCCTCTAG GTCCTCGGGG AGAAGATGGC
    CCAGAAGGTC CAAAGGGAAG ATCTGGTCCA TCAGGAGAAT CTGGCCCAAC TGGTCTTGCT
    GGTGAAAAGG GTAAACTTGG AGTCCCAGGA TTGCCCGGAT ACCCAGGTCG ACAGGGTCCC
    AAGGGTTCTA CAGGTTTCCC TGGATTTGCA GGAGCCAACG GCGAGAAAGG AGCTCGGGGT
    GTAGCTGGCA AACCAGGTCC AAGGGGACAA CGAGGTCCAA CGGGTCCACG TGGTGCTAGA
    GGTCCAAGAG GTCCTACAGG AAAACCAGGT CCTAAGGGCA CAGCAGGCAA TGATGGTCCA
    CCTGGCCCAC CTGGTGAAAG AGGACCTCAA GGACCACAGG GCCCAGTTGG GTTCCCAGGA
    CCGAAGGGCC CCCCTGGACC TCCTGGAAAG GATGGATTGC CTGGCCACCC TGGACAGCGA
    GGAGAAACAG GTTTCCAAGG AAAAACTGGG CCTCCTGGTC CTGGAGGTGT AGTAGGTCCA
    CAGGGTCCCA CCGGAGAAAC TGGTCCCATT GGTGAAAGAG GGCATCCTGG CCCACCAGGT
    CCCCCTGGAG AGCAAGGTTT ACCTGGATCA GCCGGAAAAG AGGGTGCTAA GGGAGATCCT
    GGTCCTTCAG GTGTGACTGG AAAAGATGGT CCCTCAGGTC TTCGTGGCTT CCCTGGTGAG
    AGAGGCCTAC CTGGAGCTTC GGGTGCTGCA GGTTTGAAGG GTGGAGAAGG ACCCCAGGGC
    CCACCTGGTC CACTTGGATC ACCAGGTGAG AGAGGAGCTG CCGGCGCTGC TGGACCAATT
    GGTCTGCCAG GAAGACCAGG ACCTCAAGGC CCACCAGGAC CAGCAGGAGA GAAGGGGGCT
    CCAGGAGAGA AAGGACCTCA GGGACCAGCA GGACGTGATG GAATACAAGG CCCAGTGGGA
    CTTCCAGGAC CTGCAGGTCC CCAAGGACCA CCTGGGGAAG ATGGTGACAA GGGTGAAATT
    GGAGAACCAG GACAGAAAGG AGGAAAGGGT GACAAAGGAG AACAGGGTCC TCCTGGACCA
    GGAGGTCTAC AAGGTCCTGT TGGAGCACCA GGTCCAGCGG GTTCTGATGG AGAGCCAGGT
    CCTAGAGGTC AACAGGGAAT GTTTGGGCAA AAGGGAGATG AAGGTTCTCG TGGATTCCCA
    GGTCCACCTG GCCCAATTGG TCTCCAAGGA CTTCCTGGAC CCCCAGGTGA AAAGGGTGAA
    AATGGAGATG TTGGTCCTAT GGGTCCACCC GGTCCCCCTG GTCCTAGAGG TCCTCAAGGC
    CCTAATGGAG CTGATGGTCC TCAAGGTCCT CCTGGATCTA TTGGATCTGT TGGTCCAGTT
    GGTGAAAAGG GTGAACCAGG AGAAGCTGGA AACCCAGGTC CCCCAGGAGA AGCTGGTGTT
    GGCGGTCCTA AAGGTGAAAG GGGAGAGAAA GGAGAGGCTG GACCCCCAGG AGCTGCAGGA
    CCTGCTGGTC CCAAGGGACC TCCAGGAGAT GATGGTCCTA AGGGAAATCC CGGTCCTGTT
    GGATTCCCAG GAGATCCTGG TCCACCTGGA GAACCTGGTC CAAGTGGACA AGATGGTGTT
    TCTGGTGATA AAGGAGAGGA TGGAGAATCA GGACAGCCTG GTCCACCTGG TCCCTCTGGA
    GAAGCTGGAC CACCAGGACC TCCAGGAAAG AGGGGTCCTC CTGGTGCTAA TGGTGCAGAA
    GGAAGACAAG GAGAGAAAGG TGCAAAGGGT GAGGCTGGAG CAGAAGGAGC ACCTGGTAAG
    ACTGGTCCTG TTGGACCACA AGGACCTGCT GGGAAACCAG GACCTGAAGG ACTTAGAGGA
    ATTCCAGGGC CTGTGGGAGA ACAAGGACTT CCAGGTGCCC CTGGGCAGGA TGGACCTCCA
    GGTCCAATGG GTCCTCCCGG TTTACCAGGT CTAAAAGGAG ATCCCGGTAC TAAGGGTGAG
    AAGGGACATC CAGGTCTAAT TGGTCTGATT GGGCCACCAG GAGAGCAGGG TGAAAAGGGT
    GATAGAGGAC TTCCCGGTTT ACAAGGTTCT CACGGAACCA AAGGAGATAT TGGTATATCA
    GGCCCTGCTG GTCCCACAGG TCCACCTGGA CCCCCTGGTT TACCCGGTCC TCAAGGTCCG
    AAGGGTTCAA AGGGTTCATC TGGTCCAGCT GGTCAGAAGG GAGATGGTGG TCTTCCTGGA
    CCTCCTGGTC CACCTGGCCC TCCTGGGGAA GTGATCCAAC CATTACCTAT CCAGTCACCA
    AAGAAGACCC GCAGATCTTC AGATGATATG ATGGCAGATG CTGGAGACAA CAACTTGGAG
    TATGCAGATG GAATGGAGGA GATTTTTGGA TCCCTCACTT CATTAAAGCA AGATATCGAG
    AGCATGAAAT TCCCCATAGG AAGTCAGAGC AACCCAGCAA GGACATGCAA AGACCTGCAG
    CTCTGTCACC CAGAACTCCC TGATGGTGAA TACTGGATTG ATCCAAACCA GGGCTGCTCT
    GGTGATTCAT TCAAGGTCTA CTGCAATTTC ACAGCTGGAG GAGAGACTTG TATAAATCCA
    GACAAGAAAT CAGAAGGAGT GCGCATATCA TCCTGGCCCA AAGAAAACCC TGGCTCATGG
    TTCAGTGAGT TCAAGAGAGG AAAGCTGCTT TCTTATGTGG ACATTCAGGG TAACGCTATT
    AACCAAGTAC AAATGACCTT CCTAAAACTC CTCAGTCAAT CTGCCCGGCA AAACTTCACC
    TATACTTGTC AACAATCTGT TGCTTGGTAT GACAGTGTGG GCAAGCACTA TGACAAGGCC
    CTAAGGTTCC TTGGATCCAA CGACGAGGAA ATTTCTTATG ACAACAGCCC ATACATTAGA
    CCTTTGCATG ATGGCTGTGC GCTCAAGAAA GGATTTGCAC GAACAGTCCT TGAAATTAAT
    ACCCTGAAGA TAGATCAGCT GCCCATTGCA GATGTAATGA TCAGTGACTT TGGGGATCAG
    AACCAGAAAT TTGGATTTGA GGTCGGACCG GTCTGCTTCT TAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756883.1
    CDS300..5807
    Translation

    Target ORF information:

    RefSeq Version XM_031901023.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGA AGAAGAAAGC CAGTTCCAAA TCGAAGAAGA AGCCAGTGCC CCCTAAATCA
    AAGGACAAAC CCCAAAAGGT CACGCCCAAA AACTCATTTG CAGCTAAGAA GAAGAAGGGC
    AAGACCGATT ACCAGGCGTC AGCAAGAAAA AGAATAGGGG TAAAGGCCAA TGTGGTTGAC
    TACAATCAAT ACAGTGAAGG AGACTATAGT TATCAGACAG ATACTCCTGC CCTTACTACA
    GGACCCAATG AGCAAAAACC AGAGGAGGAG TCTTTGGTGG AGGAATCCAT TGTGGAGGAA
    TCCATTGTGG AGGACCCATT TGTGGAGGAG TACGTAACAG GAGAGGACTA TGAATCAGAG
    AAAAAGCTGG TTGAGGAAAC AGAGTATGGG GTAAAAACTG TTAGCCCCAC AGAGTCTGAG
    CCAGTAGTAG GAGAAGGTTT AGGAGAATAT GATATTTATG ACTATAAGGA GTATGAAGAG
    AAGCTCCCCA ACCCTACCAC ACAAGAGGTC GGCCCTGGTG TGCCTGCTGA AACTGATATC
    TCTGAAACCA GTGTGACCAA TTATGGTGGG TATGGCGAGA AAGGCCAGAA AGGAGAGCCA
    GCAGTTATTG AGCCGGGAAT GCTGATTGAA GGCCCCCCTG GTTTGGAGGG ACCTGCTGGT
    CTTTCTGGTC CACCTGGTTT GCAAGGACCC CCTGGTATTG CTGGAGATCC TGGTGAGAGA
    GGTCCACCTG GTCGCGCAGG GTTACCGGGT GCTGATGGGT TACCAGGGCC TCCAGGCACC
    ATGCTGATGT TACCGTTCCG ATATGGTGGT GATGGAGAAA AAGGACCAAC AATCTCAGCC
    CAAGAAGCAC AGGCTCAGGC CATTCTTCAA CAAGCTCGGA TTGCCATGCG TGGACCACCA
    GGCCCAATGG GACTTACTGG AAGATCTGGC CCTGTGGGAG CACCTGGAGC TGCAGGAATT
    AAGGGAGACC CAGGAGACCC AGGCCCTCAG GGTCCTAGAG GAATTCAGGG AACACTTGGA
    TCCATGGGAA AATCTGGAAA AAGGGGACGC TCTGGAGCTG ATGGAGCCCG TGGAATGCCA
    GGAGAGCCTG GGTCCAAGGG TGACAGAGGT TTCGATGGTC TTCCAGGTCT CCCCGGGGAG
    AAGGGACACA GGGGTGACAA AGGTCCTCAT GGGCCACCAG GGCCTCCTGG TGAAGATGGA
    GAGAGGGGAG AAGATGGAGA AGTTGGACCA AGAGGGCTTC CCGGTGAACC TGGTGAAAGA
    GGACTATTAG GACCAAGGGG ACCACCAGGA CCTTCAGGAC AGCCTGGTGT TGCTGGTGTG
    GATGGACCTC AAGGCCCCAA AGGAAACATG GGCCCTCAAG GAGAGCCTGG CCCTGCAGGT
    CAACAAGGAA TTCCAGGCCC CCAAGGTCTC CCTGGTCCAC AAGGCTCCAT CGGTCCACCT
    GGTGAGAAGG GTCCACAAGG AAAGCCTGGT TTGCCTGGAT TAGCTGGAGC TGACGGACCC
    CCTGGACATC CTGGTAAAGA AGGCCCCCCA GGAGAGAAGG GAGCATTGGG TCCCACTGGT
    CCCCAAGGTC CGATTGGTTA CCCTGGACCT CGCGGTGTTA AGGGAGCAGA TGGTGTCCGA
    GGATTGAAAG GAGGCAAAGG AGAAAAGGGT GAAGATGGAT TTCCAGGATT TAAGGGTGAC
    ATGGGTCTTA AAGGAGACAG AGGAGAAGTT GGTCCTCTAG GTCCTCGGGG AGAAGATGGC
    CCAGAAGGTC CAAAGGGAAG ATCTGGTCCA TCAGGAGAAT CTGGCCCAAC TGGTCTTGCT
    GGTGAAAAGG GTAAACTTGG AGTCCCAGGA TTGCCCGGAT ACCCAGGTCG ACAGGGTCCC
    AAGGGTTCTA CAGGTTTCCC TGGATTTGCA GGAGCCAACG GCGAGAAAGG AGCTCGGGGT
    GTAGCTGGCA AACCAGGTCC AAGGGGACAA CGAGGTCCAA CGGGTCCACG TGGTGCTAGA
    GGTCCAAGAG GTCCTACAGG AAAACCAGGT CCTAAGGGCA CAGCAGGCAA TGATGGTCCA
    CCTGGCCCAC CTGGTGAAAG AGGACCTCAA GGACCACAGG GCCCAGTTGG GTTCCCAGGA
    CCGAAGGGCC CCCCTGGACC TCCTGGAAAG GATGGATTGC CTGGCCACCC TGGACAGCGA
    GGAGAAACAG GTTTCCAAGG AAAAACTGGG CCTCCTGGTC CTGGAGGTGT AGTAGGTCCA
    CAGGGTCCCA CCGGAGAAAC TGGTCCCATT GGTGAAAGAG GGCATCCTGG CCCACCAGGT
    CCCCCTGGAG AGCAAGGTTT ACCTGGATCA GCCGGAAAAG AGGGTGCTAA GGGAGATCCT
    GGTCCTTCAG GTGTGACTGG AAAAGATGGT CCCTCAGGTC TTCGTGGCTT CCCTGGTGAG
    AGAGGCCTAC CTGGAGCTTC GGGTGCTGCA GGTTTGAAGG GTGGAGAAGG ACCCCAGGGC
    CCACCTGGTC CACTTGGATC ACCAGGTGAG AGAGGAGCTG CCGGCGCTGC TGGACCAATT
    GGTCTGCCAG GAAGACCAGG ACCTCAAGGC CCACCAGGAC CAGCAGGAGA GAAGGGGGCT
    CCAGGAGAGA AAGGACCTCA GGGACCAGCA GGACGTGATG GAATACAAGG CCCAGTGGGA
    CTTCCAGGAC CTGCAGGTCC CCAAGGACCA CCTGGGGAAG ATGGTGACAA GGGTGAAATT
    GGAGAACCAG GACAGAAAGG AGGAAAGGGT GACAAAGGAG AACAGGGTCC TCCTGGACCA
    GGAGGTCTAC AAGGTCCTGT TGGAGCACCA GGTCCAGCGG GTTCTGATGG AGAGCCAGGT
    CCTAGAGGTC AACAGGGAAT GTTTGGGCAA AAGGGAGATG AAGGTTCTCG TGGATTCCCA
    GGTCCACCTG GCCCAATTGG TCTCCAAGGA CTTCCTGGAC CCCCAGGTGA AAAGGGTGAA
    AATGGAGATG TTGGTCCTAT GGGTCCACCC GGTCCCCCTG GTCCTAGAGG TCCTCAAGGC
    CCTAATGGAG CTGATGGTCC TCAAGGTCCT CCTGGATCTA TTGGATCTGT TGGTCCAGTT
    GGTGAAAAGG GTGAACCAGG AGAAGCTGGA AACCCAGGTC CCCCAGGAGA AGCTGGTGTT
    GGCGGTCCTA AAGGTGAAAG GGGAGAGAAA GGAGAGGCTG GACCCCCAGG AGCTGCAGGA
    CCTGCTGGTC CCAAGGGACC TCCAGGAGAT GATGGTCCTA AGGGAAATCC CGGTCCTGTT
    GGATTCCCAG GAGATCCTGG TCCACCTGGA GAACCTGGTC CAAGTGGACA AGATGGTGTT
    TCTGGTGATA AAGGAGAGGA TGGAGAATCA GGACAGCCTG GTCCACCTGG TCCCTCTGGA
    GAAGCTGGAC CACCAGGACC TCCAGGAAAG AGGGGTCCTC CTGGTGCTAA TGGTGCAGAA
    GGAAGACAAG GAGAGAAAGG TGCAAAGGGT GAGGCTGGAG CAGAAGGAGC ACCTGGTAAG
    ACTGGTCCTG TTGGACCACA AGGACCTGCT GGGAAACCAG GACCTGAAGG ACTTAGAGGA
    ATTCCAGGGC CTGTGGGAGA ACAAGGACTT CCAGGTGCCC CTGGGCAGGA TGGACCTCCA
    GGTCCAATGG GTCCTCCCGG TTTACCAGGT CTAAAAGGAG ATCCCGGTAC TAAGGGTGAG
    AAGGGACATC CAGGTCTAAT TGGTCTGATT GGGCCACCAG GAGAGCAGGG TGAAAAGGGT
    GATAGAGGAC TTCCCGGTTT ACAAGGTTCT CACGGAACCA AAGGAGATAT TGGTATATCA
    GGCCCTGCTG GTCCCACAGG TCCACCTGGA CCCCCTGGTT TACCCGGTCC TCAAGGTCCG
    AAGGGTTCAA AGGGTTCATC TGGTCCAGCT GGTCAGAAGG GAGATGGTGG TCTTCCTGGA
    CCTCCTGGTC CACCTGGCCC TCCTGGGGAA GTGATCCAAC CATTACCTAT CCAGTCACCA
    AAGAAGACCC GCAGATCTTC AGATGATATG ATGGCAGATG CTGGAGACAA CAACTTGGAG
    TATGCAGATG GAATGGAGGA GATTTTTGGA TCCCTCACTT CATTAAAGCA AGATATCGAG
    AGCATGAAAT TCCCCATAGG AAGTCAGAGC AACCCAGCAA GGACATGCAA AGACCTGCAG
    CTCTGTCACC CAGAACTCCC TGATGGTGAA TACTGGATTG ATCCAAACCA GGGCTGCTCT
    GGTGATTCAT TCAAGGTCTA CTGCAATTTC ACAGCTGGAG GAGAGACTTG TATAAATCCA
    GACAAGAAAT CAGAAGGAGT GCGCATATCA TCCTGGCCCA AAGAAAACCC TGGCTCATGG
    TTCAGTGAGT TCAAGAGAGG AAAGCTGCTT TCTTATGTGG ACATTCAGGG TAACGCTATT
    AACCAAGTAC AAATGACCTT CCTAAAACTC CTCAGTCAAT CTGCCCGGCA AAACTTCACC
    TATACTTGTC AACAATCTGT TGCTTGGTAT GACAGTGTGG GCAAGCACTA TGACAAGGCC
    CTAAGGTTCC TTGGATCCAA CGACGAGGAA ATTTCTTATG ACAACAGCCC ATACATTAGA
    CCTTTGCATG ATGGCTGTGC GCTCAAGAAA GGATTTGCAC GAACAGTCCT TGAAATTAAT
    ACCCTGAAGA TAGATCAGCT GCCCATTGCA GATGTAATGA TCAGTGACTT TGGGGATCAG
    AACCAGAAAT TTGGATTTGA GGTCGGACCG GTCTGCTTCT TAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57309
    Clone ID Related Accession (Same CDS sequence) XM_004913714.4
    Accession Version XM_004913714.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913714.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGGCCA ATGTGGTTGA CTACAATCAA TACAGTGAAG GAGACTATAG TTATCAGACA
    GATACTCCTG CCCTTACTAC AGGACCCAAT GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG
    GAGGAATCCA TTGTGGAGGA ATCCATTGTG GAGGACCCAT TTGTGGAGGA GTACGTAACA
    GGAGAGGACT ATGAATCAGA GAAAAAGCTG GTTGAGGAAA CAGAGTATGG GGTAAAAACT
    GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA GGAGAAGGTT TAGGAGAATA TGATATTTAT
    GACTATAAGG AGTATGAAGA GAAGCTCCCC AACCCTACCA CACAAGAGGT CGGCCCTGGT
    GTGCCTGCTG AAACTGATAT CTCTGAAACC AGTGTGACCA ATTATGGTGG GTATGGCGAG
    AAAGGCCAGA AAGGAGAGCC AGCAGTTATT GAGCCGGGAA TGCTGATTGA AGGCCCCCCT
    GGTTTGGAGG GACCTGCTGG TCTTTCTGGT CCACCTGGTT TGCAAGGACC CCCTGGTATT
    GCTGGAGATC CTGGTGAGAG AGGTCCACCT GGTCGCGCAG GGTTACCGGG TGCTGATGGG
    TTACCAGGGC CTCCAGGCAC CATGCTGATG TTACCGTTCC GATATGGTGG TGATGGAGAA
    AAAGGACCAA CAATCTCAGC CCAAGAAGCA CAGGCTCAGG CCATTCTTCA ACAAGCTCGG
    ATTGCCATGC GTGGACCACC AGGCCCAATG GGACTTACTG GAAGATCTGG CCCTGTGGGA
    GCACCTGGAG CTGCAGGAAT TAAGGGAGAC CCAGGAGACC CAGGCCCTCA GGGTCCTAGA
    GGAATTCAGG GAACACTTGG ATCCATGGGA AAATCTGGAA AAAGGGGACG CTCTGGAGCT
    GATGGAGCCC GTGGAATGCC AGGAGAGCCT GGGTCCAAGG GTGACAGAGG TTTCGATGGT
    CTTCCAGGTC TCCCCGGGGA GAAGGGACAC AGGGGTGACA AAGGTCCTCA TGGGCCACCA
    GGGCCTCCTG GTGAAGATGG AGAGAGGGGA GAAGATGGAG AAGTTGGACC AAGAGGGCTT
    CCCGGTGAAC CTGGTGAAAG AGGACTATTA GGACCAAGGG GACCACCAGG ACCTTCAGGA
    CAGCCTGGTG TTGCTGGTGT GGATGGACCT CAAGGCCCCA AAGGAAACAT GGGCCCTCAA
    GGAGAGCCTG GCCCTGCAGG TCAACAAGGA ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA
    CAAGGCTCCA TCGGTCCACC TGGTGAGAAG GGTCCACAAG GAAAGCCTGG TTTGCCTGGA
    TTAGCTGGAG CTGACGGACC CCCTGGACAT CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG
    GGAGCATTGG GTCCCACTGG TCCCCAAGGT CCGATTGGTT ACCCTGGACC TCGCGGTGTT
    AAGGGAGCAG ATGGTGTCCG AGGATTGAAA GGAGGCAAAG GAGAAAAGGG TGAAGATGGA
    TTTCCAGGAT TTAAGGGTGA CATGGGTCTT AAAGGAGACA GAGGAGAAGT TGGTCCTCTA
    GGTCCTCGGG GAGAAGATGG CCCAGAAGGT CCAAAGGGAA GATCTGGTCC ATCAGGAGAA
    TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG GGTAAACTTG GAGTCCCAGG ATTGCCCGGA
    TACCCAGGTC GACAGGGTCC CAAGGGTTCT ACAGGTTTCC CTGGATTTGC AGGAGCCAAC
    GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC AAACCAGGTC CAAGGGGACA ACGAGGTCCA
    ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA GGTCCTACAG GAAAACCAGG TCCTAAGGGC
    ACAGCAGGCA ATGATGGTCC ACCTGGCCCA CCTGGTGAAA GAGGACCTCA AGGACCACAG
    GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC CCCCCTGGAC CTCCTGGAAA GGATGGATTG
    CCTGGCCACC CTGGACAGCG AGGAGAAACA GGTTTCCAAG GAAAAACTGG GCCTCCTGGT
    CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA
    GGGCATCCTG GCCCACCAGG TCCCCCTGGA GAGCAAGGTT TACCTGGATC AGCCGGAAAA
    GAGGGTGCTA AGGGAGATCC TGGTCCTTCA GGTGTGACTG GAAAAGATGG TCCCTCAGGT
    CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG
    GGTGGAGAAG GACCCCAGGG CCCACCTGGT CCACTTGGAT CACCAGGTGA GAGAGGAGCT
    GCCGGCGCTG CTGGACCAAT TGGTCTGCCA GGAAGACCAG GACCTCAAGG CCCACCAGGA
    CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG AAAGGACCTC AGGGACCAGC AGGACGTGAT
    GGAATACAAG GCCCAGTGGG ACTTCCAGGA CCTGCAGGTC CCCAAGGACC ACCTGGGGAA
    GATGGTGACA AGGGTGAAAT TGGAGAACCA GGACAGAAAG GAGGAAAGGG TGACAAAGGA
    GAACAGGGTC CTCCTGGACC AGGAGGTCTA CAAGGTCCTG TTGGAGCACC AGGTCCAGCG
    GGTTCTGATG GAGAGCCAGG TCCTAGAGGT CAACAGGGAA TGTTTGGGCA AAAGGGAGAT
    GAAGGTTCTC GTGGATTCCC AGGTCCACCT GGCCCAATTG GTCTCCAAGG ACTTCCTGGA
    CCCCCAGGTG AAAAGGGTGA AAATGGAGAT GTTGGTCCTA TGGGTCCACC CGGTCCCCCT
    GGTCCTAGAG GTCCTCAAGG CCCTAATGGA GCTGATGGTC CTCAAGGTCC TCCTGGATCT
    ATTGGATCTG TTGGTCCAGT TGGTGAAAAG GGTGAACCAG GAGAAGCTGG AAACCCAGGT
    CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT
    GGACCCCCAG GAGCTGCAGG ACCTGCTGGT CCCAAGGGAC CTCCAGGAGA TGATGGTCCT
    AAGGGAAATC CCGGTCCTGT TGGATTCCCA GGAGATCCTG GTCCACCTGG AGAACCTGGT
    CCAAGTGGAC AAGATGGTGT TTCTGGTGAT AAAGGAGAGG ATGGAGAATC AGGACAGCCT
    GGTCCACCTG GTCCCTCTGG AGAAGCTGGA CCACCAGGAC CTCCAGGAAA GAGGGGTCCT
    CCTGGTGCTA ATGGTGCAGA AGGAAGACAA GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA
    GCAGAAGGAG CACCTGGTAA GACTGGTCCT GTTGGACCAC AAGGACCTGC TGGGAAACCA
    GGACCTGAAG GACTTAGAGG AATTCCAGGG CCTGTGGGAG AACAAGGACT TCCAGGTGCC
    CCTGGGCAGG ATGGACCTCC AGGTCCAATG GGTCCTCCCG GTTTACCAGG TCTAAAAGGA
    GATCCCGGTA CTAAGGGTGA GAAGGGACAT CCAGGTCTAA TTGGTCTGAT TGGGCCACCA
    GGAGAGCAGG GTGAAAAGGG TGATAGAGGA CTTCCCGGTT TACAAGGTTC TCACGGAACC
    AAAGGAGATA TTGGTATATC AGGCCCTGCT GGTCCCACAG GTCCACCTGG ACCCCCTGGT
    TTACCCGGTC CTCAAGGTCC GAAGGGTTCA AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG
    GGAGATGGTG GTCTTCCTGG ACCTCCTGGT CCACCTGGCC CTCCTGGGGA AGTGATCCAA
    CCATTACCTA TCCAGTCACC AAAGAAGACC CGCAGATCTT CAGATGATAT GATGGCAGAT
    GCTGGAGACA ACAACTTGGA GTATGCAGAT GGAATGGAGG AGATTTTTGG ATCCCTCACT
    TCATTAAAGC AAGATATCGA GAGCATGAAA TTCCCCATAG GAAGTCAGAG CAACCCAGCA
    AGGACATGCA AAGACCTGCA GCTCTGTCAC CCAGAACTCC CTGATGGTGA ATACTGGATT
    GATCCAAACC AGGGCTGCTC TGGTGATTCA TTCAAGGTCT ACTGCAATTT CACAGCTGGA
    GGAGAGACTT GTATAAATCC AGACAAGAAA TCAGAAGGAG TGCGCATATC ATCCTGGCCC
    AAAGAAAACC CTGGCTCATG GTTCAGTGAG TTCAAGAGAG GAAAGCTGCT TTCTTATGTG
    GACATTCAGG GTAACGCTAT TAACCAAGTA CAAATGACCT TCCTAAAACT CCTCAGTCAA
    TCTGCCCGGC AAAACTTCAC CTATACTTGT CAACAATCTG TTGCTTGGTA TGACAGTGTG
    GGCAAGCACT ATGACAAGGC CCTAAGGTTC CTTGGATCCA ACGACGAGGA AATTTCTTAT
    GACAACAGCC CATACATTAG ACCTTTGCAT GATGGCTGTG CGCTCAAGAA AGGATTTGCA
    CGAACAGTCC TTGAAATTAA TACCCTGAAG ATAGATCAGC TGCCCATTGC AGATGTAATG
    ATCAGTGACT TTGGGGATCA GAACCAGAAA TTTGGATTTG AGGTCGGACC GGTCTGCTTC
    TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913771.1
    CDS298..5766
    Translation

    Target ORF information:

    RefSeq Version XM_004913714.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913714.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGGCCA ATGTGGTTGA CTACAATCAA TACAGTGAAG GAGACTATAG TTATCAGACA
    GATACTCCTG CCCTTACTAC AGGACCCAAT GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG
    GAGGAATCCA TTGTGGAGGA ATCCATTGTG GAGGACCCAT TTGTGGAGGA GTACGTAACA
    GGAGAGGACT ATGAATCAGA GAAAAAGCTG GTTGAGGAAA CAGAGTATGG GGTAAAAACT
    GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA GGAGAAGGTT TAGGAGAATA TGATATTTAT
    GACTATAAGG AGTATGAAGA GAAGCTCCCC AACCCTACCA CACAAGAGGT CGGCCCTGGT
    GTGCCTGCTG AAACTGATAT CTCTGAAACC AGTGTGACCA ATTATGGTGG GTATGGCGAG
    AAAGGCCAGA AAGGAGAGCC AGCAGTTATT GAGCCGGGAA TGCTGATTGA AGGCCCCCCT
    GGTTTGGAGG GACCTGCTGG TCTTTCTGGT CCACCTGGTT TGCAAGGACC CCCTGGTATT
    GCTGGAGATC CTGGTGAGAG AGGTCCACCT GGTCGCGCAG GGTTACCGGG TGCTGATGGG
    TTACCAGGGC CTCCAGGCAC CATGCTGATG TTACCGTTCC GATATGGTGG TGATGGAGAA
    AAAGGACCAA CAATCTCAGC CCAAGAAGCA CAGGCTCAGG CCATTCTTCA ACAAGCTCGG
    ATTGCCATGC GTGGACCACC AGGCCCAATG GGACTTACTG GAAGATCTGG CCCTGTGGGA
    GCACCTGGAG CTGCAGGAAT TAAGGGAGAC CCAGGAGACC CAGGCCCTCA GGGTCCTAGA
    GGAATTCAGG GAACACTTGG ATCCATGGGA AAATCTGGAA AAAGGGGACG CTCTGGAGCT
    GATGGAGCCC GTGGAATGCC AGGAGAGCCT GGGTCCAAGG GTGACAGAGG TTTCGATGGT
    CTTCCAGGTC TCCCCGGGGA GAAGGGACAC AGGGGTGACA AAGGTCCTCA TGGGCCACCA
    GGGCCTCCTG GTGAAGATGG AGAGAGGGGA GAAGATGGAG AAGTTGGACC AAGAGGGCTT
    CCCGGTGAAC CTGGTGAAAG AGGACTATTA GGACCAAGGG GACCACCAGG ACCTTCAGGA
    CAGCCTGGTG TTGCTGGTGT GGATGGACCT CAAGGCCCCA AAGGAAACAT GGGCCCTCAA
    GGAGAGCCTG GCCCTGCAGG TCAACAAGGA ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA
    CAAGGCTCCA TCGGTCCACC TGGTGAGAAG GGTCCACAAG GAAAGCCTGG TTTGCCTGGA
    TTAGCTGGAG CTGACGGACC CCCTGGACAT CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG
    GGAGCATTGG GTCCCACTGG TCCCCAAGGT CCGATTGGTT ACCCTGGACC TCGCGGTGTT
    AAGGGAGCAG ATGGTGTCCG AGGATTGAAA GGAGGCAAAG GAGAAAAGGG TGAAGATGGA
    TTTCCAGGAT TTAAGGGTGA CATGGGTCTT AAAGGAGACA GAGGAGAAGT TGGTCCTCTA
    GGTCCTCGGG GAGAAGATGG CCCAGAAGGT CCAAAGGGAA GATCTGGTCC ATCAGGAGAA
    TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG GGTAAACTTG GAGTCCCAGG ATTGCCCGGA
    TACCCAGGTC GACAGGGTCC CAAGGGTTCT ACAGGTTTCC CTGGATTTGC AGGAGCCAAC
    GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC AAACCAGGTC CAAGGGGACA ACGAGGTCCA
    ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA GGTCCTACAG GAAAACCAGG TCCTAAGGGC
    ACAGCAGGCA ATGATGGTCC ACCTGGCCCA CCTGGTGAAA GAGGACCTCA AGGACCACAG
    GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC CCCCCTGGAC CTCCTGGAAA GGATGGATTG
    CCTGGCCACC CTGGACAGCG AGGAGAAACA GGTTTCCAAG GAAAAACTGG GCCTCCTGGT
    CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA
    GGGCATCCTG GCCCACCAGG TCCCCCTGGA GAGCAAGGTT TACCTGGATC AGCCGGAAAA
    GAGGGTGCTA AGGGAGATCC TGGTCCTTCA GGTGTGACTG GAAAAGATGG TCCCTCAGGT
    CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG
    GGTGGAGAAG GACCCCAGGG CCCACCTGGT CCACTTGGAT CACCAGGTGA GAGAGGAGCT
    GCCGGCGCTG CTGGACCAAT TGGTCTGCCA GGAAGACCAG GACCTCAAGG CCCACCAGGA
    CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG AAAGGACCTC AGGGACCAGC AGGACGTGAT
    GGAATACAAG GCCCAGTGGG ACTTCCAGGA CCTGCAGGTC CCCAAGGACC ACCTGGGGAA
    GATGGTGACA AGGGTGAAAT TGGAGAACCA GGACAGAAAG GAGGAAAGGG TGACAAAGGA
    GAACAGGGTC CTCCTGGACC AGGAGGTCTA CAAGGTCCTG TTGGAGCACC AGGTCCAGCG
    GGTTCTGATG GAGAGCCAGG TCCTAGAGGT CAACAGGGAA TGTTTGGGCA AAAGGGAGAT
    GAAGGTTCTC GTGGATTCCC AGGTCCACCT GGCCCAATTG GTCTCCAAGG ACTTCCTGGA
    CCCCCAGGTG AAAAGGGTGA AAATGGAGAT GTTGGTCCTA TGGGTCCACC CGGTCCCCCT
    GGTCCTAGAG GTCCTCAAGG CCCTAATGGA GCTGATGGTC CTCAAGGTCC TCCTGGATCT
    ATTGGATCTG TTGGTCCAGT TGGTGAAAAG GGTGAACCAG GAGAAGCTGG AAACCCAGGT
    CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT
    GGACCCCCAG GAGCTGCAGG ACCTGCTGGT CCCAAGGGAC CTCCAGGAGA TGATGGTCCT
    AAGGGAAATC CCGGTCCTGT TGGATTCCCA GGAGATCCTG GTCCACCTGG AGAACCTGGT
    CCAAGTGGAC AAGATGGTGT TTCTGGTGAT AAAGGAGAGG ATGGAGAATC AGGACAGCCT
    GGTCCACCTG GTCCCTCTGG AGAAGCTGGA CCACCAGGAC CTCCAGGAAA GAGGGGTCCT
    CCTGGTGCTA ATGGTGCAGA AGGAAGACAA GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA
    GCAGAAGGAG CACCTGGTAA GACTGGTCCT GTTGGACCAC AAGGACCTGC TGGGAAACCA
    GGACCTGAAG GACTTAGAGG AATTCCAGGG CCTGTGGGAG AACAAGGACT TCCAGGTGCC
    CCTGGGCAGG ATGGACCTCC AGGTCCAATG GGTCCTCCCG GTTTACCAGG TCTAAAAGGA
    GATCCCGGTA CTAAGGGTGA GAAGGGACAT CCAGGTCTAA TTGGTCTGAT TGGGCCACCA
    GGAGAGCAGG GTGAAAAGGG TGATAGAGGA CTTCCCGGTT TACAAGGTTC TCACGGAACC
    AAAGGAGATA TTGGTATATC AGGCCCTGCT GGTCCCACAG GTCCACCTGG ACCCCCTGGT
    TTACCCGGTC CTCAAGGTCC GAAGGGTTCA AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG
    GGAGATGGTG GTCTTCCTGG ACCTCCTGGT CCACCTGGCC CTCCTGGGGA AGTGATCCAA
    CCATTACCTA TCCAGTCACC AAAGAAGACC CGCAGATCTT CAGATGATAT GATGGCAGAT
    GCTGGAGACA ACAACTTGGA GTATGCAGAT GGAATGGAGG AGATTTTTGG ATCCCTCACT
    TCATTAAAGC AAGATATCGA GAGCATGAAA TTCCCCATAG GAAGTCAGAG CAACCCAGCA
    AGGACATGCA AAGACCTGCA GCTCTGTCAC CCAGAACTCC CTGATGGTGA ATACTGGATT
    GATCCAAACC AGGGCTGCTC TGGTGATTCA TTCAAGGTCT ACTGCAATTT CACAGCTGGA
    GGAGAGACTT GTATAAATCC AGACAAGAAA TCAGAAGGAG TGCGCATATC ATCCTGGCCC
    AAAGAAAACC CTGGCTCATG GTTCAGTGAG TTCAAGAGAG GAAAGCTGCT TTCTTATGTG
    GACATTCAGG GTAACGCTAT TAACCAAGTA CAAATGACCT TCCTAAAACT CCTCAGTCAA
    TCTGCCCGGC AAAACTTCAC CTATACTTGT CAACAATCTG TTGCTTGGTA TGACAGTGTG
    GGCAAGCACT ATGACAAGGC CCTAAGGTTC CTTGGATCCA ACGACGAGGA AATTTCTTAT
    GACAACAGCC CATACATTAG ACCTTTGCAT GATGGCTGTG CGCTCAAGAA AGGATTTGCA
    CGAACAGTCC TTGAAATTAA TACCCTGAAG ATAGATCAGC TGCCCATTGC AGATGTAATG
    ATCAGTGACT TTGGGGATCA GAACCAGAAA TTTGGATTTG AGGTCGGACC GGTCTGCTTC
    TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57310
    Clone ID Related Accession (Same CDS sequence) XM_031901024.1
    Accession Version XM_031901024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGG CCAATGTGGT TGACTACAAT CAATACAGTG AAGGAGACTA TAGTTATCAG
    ACAGATACTC CTGCCCTTAC TACAGGACCC AATGAGCAAA AACCAGAGGA GGAGTCTTTG
    GTGGAGGAAT CCATTGTGGA GGAATCCATT GTGGAGGACC CATTTGTGGA GGAGTACGTA
    ACAGGAGAGG ACTATGAATC AGAGAAAAAG CTGGTTGAGG AAACAGAGTA TGGGGTAAAA
    ACTGTTAGCC CCACAGAGTC TGAGCCAGTA GTAGGAGAAG GTTTAGGAGA ATATGATATT
    TATGACTATA AGGAGTATGA AGAGAAGCTC CCCAACCCTA CCACACAAGA GGTCGGCCCT
    GGTGTGCCTG CTGAAACTGA TATCTCTGAA ACCAGTGTGA CCAATTATGG TGGGTATGGC
    GAGAAAGGCC AGAAAGGAGA GCCAGCAGTT ATTGAGCCGG GAATGCTGAT TGAAGGCCCC
    CCTGGTTTGG AGGGACCTGC TGGTCTTTCT GGTCCACCTG GTTTGCAAGG ACCCCCTGGT
    ATTGCTGGAG ATCCTGGTGA GAGAGGTCCA CCTGGTCGCG CAGGGTTACC GGGTGCTGAT
    GGGTTACCAG GGCCTCCAGG CACCATGCTG ATGTTACCGT TCCGATATGG TGGTGATGGA
    GAAAAAGGAC CAACAATCTC AGCCCAAGAA GCACAGGCTC AGGCCATTCT TCAACAAGCT
    CGGATTGCCA TGCGTGGACC ACCAGGCCCA ATGGGACTTA CTGGAAGATC TGGCCCTGTG
    GGAGCACCTG GAGCTGCAGG AATTAAGGGA GACCCAGGAG ACCCAGGCCC TCAGGGTCCT
    AGAGGAATTC AGGGAACACT TGGATCCATG GGAAAATCTG GAAAAAGGGG ACGCTCTGGA
    GCTGATGGAG CCCGTGGAAT GCCAGGAGAG CCTGGGTCCA AGGGTGACAG AGGTTTCGAT
    GGTCTTCCAG GTCTCCCCGG GGAGAAGGGA CACAGGGGTG ACAAAGGTCC TCATGGGCCA
    CCAGGGCCTC CTGGTGAAGA TGGAGAGAGG GGAGAAGATG GAGAAGTTGG ACCAAGAGGG
    CTTCCCGGTG AACCTGGTGA AAGAGGACTA TTAGGACCAA GGGGACCACC AGGACCTTCA
    GGACAGCCTG GTGTTGCTGG TGTGGATGGA CCTCAAGGCC CCAAAGGAAA CATGGGCCCT
    CAAGGAGAGC CTGGCCCTGC AGGTCAACAA GGAATTCCAG GCCCCCAAGG TCTCCCTGGT
    CCACAAGGCT CCATCGGTCC ACCTGGTGAG AAGGGTCCAC AAGGAAAGCC TGGTTTGCCT
    GGATTAGCTG GAGCTGACGG ACCCCCTGGA CATCCTGGTA AAGAAGGCCC CCCAGGAGAG
    AAGGGAGCAT TGGGTCCCAC TGGTCCCCAA GGTCCGATTG GTTACCCTGG ACCTCGCGGT
    GTTAAGGGAG CAGATGGTGT CCGAGGATTG AAAGGAGGCA AAGGAGAAAA GGGTGAAGAT
    GGATTTCCAG GATTTAAGGG TGACATGGGT CTTAAAGGAG ACAGAGGAGA AGTTGGTCCT
    CTAGGTCCTC GGGGAGAAGA TGGCCCAGAA GGTCCAAAGG GAAGATCTGG TCCATCAGGA
    GAATCTGGCC CAACTGGTCT TGCTGGTGAA AAGGGTAAAC TTGGAGTCCC AGGATTGCCC
    GGATACCCAG GTCGACAGGG TCCCAAGGGT TCTACAGGTT TCCCTGGATT TGCAGGAGCC
    AACGGCGAGA AAGGAGCTCG GGGTGTAGCT GGCAAACCAG GTCCAAGGGG ACAACGAGGT
    CCAACGGGTC CACGTGGTGC TAGAGGTCCA AGAGGTCCTA CAGGAAAACC AGGTCCTAAG
    GGCACAGCAG GCAATGATGG TCCACCTGGC CCACCTGGTG AAAGAGGACC TCAAGGACCA
    CAGGGCCCAG TTGGGTTCCC AGGACCGAAG GGCCCCCCTG GACCTCCTGG AAAGGATGGA
    TTGCCTGGCC ACCCTGGACA GCGAGGAGAA ACAGGTTTCC AAGGAAAAAC TGGGCCTCCT
    GGTCCTGGAG GTGTAGTAGG TCCACAGGGT CCCACCGGAG AAACTGGTCC CATTGGTGAA
    AGAGGGCATC CTGGCCCACC AGGTCCCCCT GGAGAGCAAG GTTTACCTGG ATCAGCCGGA
    AAAGAGGGTG CTAAGGGAGA TCCTGGTCCT TCAGGTGTGA CTGGAAAAGA TGGTCCCTCA
    GGTCTTCGTG GCTTCCCTGG TGAGAGAGGC CTACCTGGAG CTTCGGGTGC TGCAGGTTTG
    AAGGGTGGAG AAGGACCCCA GGGCCCACCT GGTCCACTTG GATCACCAGG TGAGAGAGGA
    GCTGCCGGCG CTGCTGGACC AATTGGTCTG CCAGGAAGAC CAGGACCTCA AGGCCCACCA
    GGACCAGCAG GAGAGAAGGG GGCTCCAGGA GAGAAAGGAC CTCAGGGACC AGCAGGACGT
    GATGGAATAC AAGGCCCAGT GGGACTTCCA GGACCTGCAG GTCCCCAAGG ACCACCTGGG
    GAAGATGGTG ACAAGGGTGA AATTGGAGAA CCAGGACAGA AAGGAGGAAA GGGTGACAAA
    GGAGAACAGG GTCCTCCTGG ACCAGGAGGT CTACAAGGTC CTGTTGGAGC ACCAGGTCCA
    GCGGGTTCTG ATGGAGAGCC AGGTCCTAGA GGTCAACAGG GAATGTTTGG GCAAAAGGGA
    GATGAAGGTT CTCGTGGATT CCCAGGTCCA CCTGGCCCAA TTGGTCTCCA AGGACTTCCT
    GGACCCCCAG GTGAAAAGGG TGAAAATGGA GATGTTGGTC CTATGGGTCC ACCCGGTCCC
    CCTGGTCCTA GAGGTCCTCA AGGCCCTAAT GGAGCTGATG GTCCTCAAGG TCCTCCTGGA
    TCTATTGGAT CTGTTGGTCC AGTTGGTGAA AAGGGTGAAC CAGGAGAAGC TGGAAACCCA
    GGTCCCCCAG GAGAAGCTGG TGTTGGCGGT CCTAAAGGTG AAAGGGGAGA GAAAGGAGAG
    GCTGGACCCC CAGGAGCTGC AGGACCTGCT GGTCCCAAGG GACCTCCAGG AGATGATGGT
    CCTAAGGGAA ATCCCGGTCC TGTTGGATTC CCAGGAGATC CTGGTCCACC TGGAGAACCT
    GGTCCAAGTG GACAAGATGG TGTTTCTGGT GATAAAGGAG AGGATGGAGA ATCAGGACAG
    CCTGGTCCAC CTGGTCCCTC TGGAGAAGCT GGACCACCAG GACCTCCAGG AAAGAGGGGT
    CCTCCTGGTG CTAATGGTGC AGAAGGAAGA CAAGGAGAGA AAGGTGCAAA GGGTGAGGCT
    GGAGCAGAAG GAGCACCTGG TAAGACTGGT CCTGTTGGAC CACAAGGACC TGCTGGGAAA
    CCAGGACCTG AAGGACTTAG AGGAATTCCA GGGCCTGTGG GAGAACAAGG ACTTCCAGGT
    GCCCCTGGGC AGGATGGACC TCCAGGTCCA ATGGGTCCTC CCGGTTTACC AGGTCTAAAA
    GGAGATCCCG GTACTAAGGG TGAGAAGGGA CATCCAGGTC TAATTGGTCT GATTGGGCCA
    CCAGGAGAGC AGGGTGAAAA GGGTGATAGA GGACTTCCCG GTTTACAAGG TTCTCACGGA
    ACCAAAGGAG ATATTGGTAT ATCAGGCCCT GCTGGTCCCA CAGGTCCACC TGGACCCCCT
    GGTTTACCCG GTCCTCAAGG TCCGAAGGGT TCAAAGGGTT CATCTGGTCC AGCTGGTCAG
    AAGGGAGATG GTGGTCTTCC TGGACCTCCT GGTCCACCTG GCCCTCCTGG GGAAGTGATC
    CAACCATTAC CTATCCAGTC ACCAAAGAAG ACCCGCAGAT CTTCAGATGA TATGATGGCA
    GATGCTGGAG ACAACAACTT GGAGTATGCA GATGGAATGG AGGAGATTTT TGGATCCCTC
    ACTTCATTAA AGCAAGATAT CGAGAGCATG AAATTCCCCA TAGGAAGTCA GAGCAACCCA
    GCAAGGACAT GCAAAGACCT GCAGCTCTGT CACCCAGAAC TCCCTGATGG TGAATACTGG
    ATTGATCCAA ACCAGGGCTG CTCTGGTGAT TCATTCAAGG TCTACTGCAA TTTCACAGCT
    GGAGGAGAGA CTTGTATAAA TCCAGACAAG AAATCAGAAG GAGTGCGCAT ATCATCCTGG
    CCCAAAGAAA ACCCTGGCTC ATGGTTCAGT GAGTTCAAGA GAGGAAAGCT GCTTTCTTAT
    GTGGACATTC AGGGTAACGC TATTAACCAA GTACAAATGA CCTTCCTAAA ACTCCTCAGT
    CAATCTGCCC GGCAAAACTT CACCTATACT TGTCAACAAT CTGTTGCTTG GTATGACAGT
    GTGGGCAAGC ACTATGACAA GGCCCTAAGG TTCCTTGGAT CCAACGACGA GGAAATTTCT
    TATGACAACA GCCCATACAT TAGACCTTTG CATGATGGCT GTGCGCTCAA GAAAGGATTT
    GCACGAACAG TCCTTGAAAT TAATACCCTG AAGATAGATC AGCTGCCCAT TGCAGATGTA
    ATGATCAGTG ACTTTGGGGA TCAGAACCAG AAATTTGGAT TTGAGGTCGG ACCGGTCTGC
    TTCTTAGGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756884.1
    CDS299..5650
    Translation

    Target ORF information:

    RefSeq Version XM_031901024.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGG CCAATGTGGT TGACTACAAT CAATACAGTG AAGGAGACTA TAGTTATCAG
    ACAGATACTC CTGCCCTTAC TACAGGACCC AATGAGCAAA AACCAGAGGA GGAGTCTTTG
    GTGGAGGAAT CCATTGTGGA GGAATCCATT GTGGAGGACC CATTTGTGGA GGAGTACGTA
    ACAGGAGAGG ACTATGAATC AGAGAAAAAG CTGGTTGAGG AAACAGAGTA TGGGGTAAAA
    ACTGTTAGCC CCACAGAGTC TGAGCCAGTA GTAGGAGAAG GTTTAGGAGA ATATGATATT
    TATGACTATA AGGAGTATGA AGAGAAGCTC CCCAACCCTA CCACACAAGA GGTCGGCCCT
    GGTGTGCCTG CTGAAACTGA TATCTCTGAA ACCAGTGTGA CCAATTATGG TGGGTATGGC
    GAGAAAGGCC AGAAAGGAGA GCCAGCAGTT ATTGAGCCGG GAATGCTGAT TGAAGGCCCC
    CCTGGTTTGG AGGGACCTGC TGGTCTTTCT GGTCCACCTG GTTTGCAAGG ACCCCCTGGT
    ATTGCTGGAG ATCCTGGTGA GAGAGGTCCA CCTGGTCGCG CAGGGTTACC GGGTGCTGAT
    GGGTTACCAG GGCCTCCAGG CACCATGCTG ATGTTACCGT TCCGATATGG TGGTGATGGA
    GAAAAAGGAC CAACAATCTC AGCCCAAGAA GCACAGGCTC AGGCCATTCT TCAACAAGCT
    CGGATTGCCA TGCGTGGACC ACCAGGCCCA ATGGGACTTA CTGGAAGATC TGGCCCTGTG
    GGAGCACCTG GAGCTGCAGG AATTAAGGGA GACCCAGGAG ACCCAGGCCC TCAGGGTCCT
    AGAGGAATTC AGGGAACACT TGGATCCATG GGAAAATCTG GAAAAAGGGG ACGCTCTGGA
    GCTGATGGAG CCCGTGGAAT GCCAGGAGAG CCTGGGTCCA AGGGTGACAG AGGTTTCGAT
    GGTCTTCCAG GTCTCCCCGG GGAGAAGGGA CACAGGGGTG ACAAAGGTCC TCATGGGCCA
    CCAGGGCCTC CTGGTGAAGA TGGAGAGAGG GGAGAAGATG GAGAAGTTGG ACCAAGAGGG
    CTTCCCGGTG AACCTGGTGA AAGAGGACTA TTAGGACCAA GGGGACCACC AGGACCTTCA
    GGACAGCCTG GTGTTGCTGG TGTGGATGGA CCTCAAGGCC CCAAAGGAAA CATGGGCCCT
    CAAGGAGAGC CTGGCCCTGC AGGTCAACAA GGAATTCCAG GCCCCCAAGG TCTCCCTGGT
    CCACAAGGCT CCATCGGTCC ACCTGGTGAG AAGGGTCCAC AAGGAAAGCC TGGTTTGCCT
    GGATTAGCTG GAGCTGACGG ACCCCCTGGA CATCCTGGTA AAGAAGGCCC CCCAGGAGAG
    AAGGGAGCAT TGGGTCCCAC TGGTCCCCAA GGTCCGATTG GTTACCCTGG ACCTCGCGGT
    GTTAAGGGAG CAGATGGTGT CCGAGGATTG AAAGGAGGCA AAGGAGAAAA GGGTGAAGAT
    GGATTTCCAG GATTTAAGGG TGACATGGGT CTTAAAGGAG ACAGAGGAGA AGTTGGTCCT
    CTAGGTCCTC GGGGAGAAGA TGGCCCAGAA GGTCCAAAGG GAAGATCTGG TCCATCAGGA
    GAATCTGGCC CAACTGGTCT TGCTGGTGAA AAGGGTAAAC TTGGAGTCCC AGGATTGCCC
    GGATACCCAG GTCGACAGGG TCCCAAGGGT TCTACAGGTT TCCCTGGATT TGCAGGAGCC
    AACGGCGAGA AAGGAGCTCG GGGTGTAGCT GGCAAACCAG GTCCAAGGGG ACAACGAGGT
    CCAACGGGTC CACGTGGTGC TAGAGGTCCA AGAGGTCCTA CAGGAAAACC AGGTCCTAAG
    GGCACAGCAG GCAATGATGG TCCACCTGGC CCACCTGGTG AAAGAGGACC TCAAGGACCA
    CAGGGCCCAG TTGGGTTCCC AGGACCGAAG GGCCCCCCTG GACCTCCTGG AAAGGATGGA
    TTGCCTGGCC ACCCTGGACA GCGAGGAGAA ACAGGTTTCC AAGGAAAAAC TGGGCCTCCT
    GGTCCTGGAG GTGTAGTAGG TCCACAGGGT CCCACCGGAG AAACTGGTCC CATTGGTGAA
    AGAGGGCATC CTGGCCCACC AGGTCCCCCT GGAGAGCAAG GTTTACCTGG ATCAGCCGGA
    AAAGAGGGTG CTAAGGGAGA TCCTGGTCCT TCAGGTGTGA CTGGAAAAGA TGGTCCCTCA
    GGTCTTCGTG GCTTCCCTGG TGAGAGAGGC CTACCTGGAG CTTCGGGTGC TGCAGGTTTG
    AAGGGTGGAG AAGGACCCCA GGGCCCACCT GGTCCACTTG GATCACCAGG TGAGAGAGGA
    GCTGCCGGCG CTGCTGGACC AATTGGTCTG CCAGGAAGAC CAGGACCTCA AGGCCCACCA
    GGACCAGCAG GAGAGAAGGG GGCTCCAGGA GAGAAAGGAC CTCAGGGACC AGCAGGACGT
    GATGGAATAC AAGGCCCAGT GGGACTTCCA GGACCTGCAG GTCCCCAAGG ACCACCTGGG
    GAAGATGGTG ACAAGGGTGA AATTGGAGAA CCAGGACAGA AAGGAGGAAA GGGTGACAAA
    GGAGAACAGG GTCCTCCTGG ACCAGGAGGT CTACAAGGTC CTGTTGGAGC ACCAGGTCCA
    GCGGGTTCTG ATGGAGAGCC AGGTCCTAGA GGTCAACAGG GAATGTTTGG GCAAAAGGGA
    GATGAAGGTT CTCGTGGATT CCCAGGTCCA CCTGGCCCAA TTGGTCTCCA AGGACTTCCT
    GGACCCCCAG GTGAAAAGGG TGAAAATGGA GATGTTGGTC CTATGGGTCC ACCCGGTCCC
    CCTGGTCCTA GAGGTCCTCA AGGCCCTAAT GGAGCTGATG GTCCTCAAGG TCCTCCTGGA
    TCTATTGGAT CTGTTGGTCC AGTTGGTGAA AAGGGTGAAC CAGGAGAAGC TGGAAACCCA
    GGTCCCCCAG GAGAAGCTGG TGTTGGCGGT CCTAAAGGTG AAAGGGGAGA GAAAGGAGAG
    GCTGGACCCC CAGGAGCTGC AGGACCTGCT GGTCCCAAGG GACCTCCAGG AGATGATGGT
    CCTAAGGGAA ATCCCGGTCC TGTTGGATTC CCAGGAGATC CTGGTCCACC TGGAGAACCT
    GGTCCAAGTG GACAAGATGG TGTTTCTGGT GATAAAGGAG AGGATGGAGA ATCAGGACAG
    CCTGGTCCAC CTGGTCCCTC TGGAGAAGCT GGACCACCAG GACCTCCAGG AAAGAGGGGT
    CCTCCTGGTG CTAATGGTGC AGAAGGAAGA CAAGGAGAGA AAGGTGCAAA GGGTGAGGCT
    GGAGCAGAAG GAGCACCTGG TAAGACTGGT CCTGTTGGAC CACAAGGACC TGCTGGGAAA
    CCAGGACCTG AAGGACTTAG AGGAATTCCA GGGCCTGTGG GAGAACAAGG ACTTCCAGGT
    GCCCCTGGGC AGGATGGACC TCCAGGTCCA ATGGGTCCTC CCGGTTTACC AGGTCTAAAA
    GGAGATCCCG GTACTAAGGG TGAGAAGGGA CATCCAGGTC TAATTGGTCT GATTGGGCCA
    CCAGGAGAGC AGGGTGAAAA GGGTGATAGA GGACTTCCCG GTTTACAAGG TTCTCACGGA
    ACCAAAGGAG ATATTGGTAT ATCAGGCCCT GCTGGTCCCA CAGGTCCACC TGGACCCCCT
    GGTTTACCCG GTCCTCAAGG TCCGAAGGGT TCAAAGGGTT CATCTGGTCC AGCTGGTCAG
    AAGGGAGATG GTGGTCTTCC TGGACCTCCT GGTCCACCTG GCCCTCCTGG GGAAGTGATC
    CAACCATTAC CTATCCAGTC ACCAAAGAAG ACCCGCAGAT CTTCAGATGA TATGATGGCA
    GATGCTGGAG ACAACAACTT GGAGTATGCA GATGGAATGG AGGAGATTTT TGGATCCCTC
    ACTTCATTAA AGCAAGATAT CGAGAGCATG AAATTCCCCA TAGGAAGTCA GAGCAACCCA
    GCAAGGACAT GCAAAGACCT GCAGCTCTGT CACCCAGAAC TCCCTGATGG TGAATACTGG
    ATTGATCCAA ACCAGGGCTG CTCTGGTGAT TCATTCAAGG TCTACTGCAA TTTCACAGCT
    GGAGGAGAGA CTTGTATAAA TCCAGACAAG AAATCAGAAG GAGTGCGCAT ATCATCCTGG
    CCCAAAGAAA ACCCTGGCTC ATGGTTCAGT GAGTTCAAGA GAGGAAAGCT GCTTTCTTAT
    GTGGACATTC AGGGTAACGC TATTAACCAA GTACAAATGA CCTTCCTAAA ACTCCTCAGT
    CAATCTGCCC GGCAAAACTT CACCTATACT TGTCAACAAT CTGTTGCTTG GTATGACAGT
    GTGGGCAAGC ACTATGACAA GGCCCTAAGG TTCCTTGGAT CCAACGACGA GGAAATTTCT
    TATGACAACA GCCCATACAT TAGACCTTTG CATGATGGCT GTGCGCTCAA GAAAGGATTT
    GCACGAACAG TCCTTGAAAT TAATACCCTG AAGATAGATC AGCTGCCCAT TGCAGATGTA
    ATGATCAGTG ACTTTGGGGA TCAGAACCAG AAATTTGGAT TTGAGGTCGG ACCGGTCTGC
    TTCTTAGGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57311
    Clone ID Related Accession (Same CDS sequence) XM_031901025.1
    Accession Version XM_031901025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGTAA AGGCCAATGT GGTTGACTAC
    AATCAATACA GTGAAGGAGA CTATAGTTAT CAGACAGATA CTCCTGCCCT TACTACAGGA
    CCCAATGAGG TGACCAATTA TGGTGGGTAT GGCGAGAAAG GCCAGAAAGG AGAGCCAGCA
    GTTATTGAGC CGGGAATGCT GATTGAAGGC CCCCCTGGTT TGGAGGGACC TGCTGGTCTT
    TCTGGTCCAC CTGGTTTGCA AGGACCCCCT GGTATTGCTG GAGATCCTGG TGAGAGAGGT
    CCACCTGGTC GCGCAGGGTT ACCGGGTGCT GATGGGTTAC CAGGGCCTCC AGGCACCATG
    CTGATGTTAC CGTTCCGATA TGGTGGTGAT GGAGAAAAAG GACCAACAAT CTCAGCCCAA
    GAAGCACAGG CTCAGGCCAT TCTTCAACAA GCTCGGATTG CCATGCGTGG ACCACCAGGC
    CCAATGGGAC TTACTGGAAG ATCTGGCCCT GTGGGAGCAC CTGGAGCTGC AGGAATTAAG
    GGAGACCCAG GAGACCCAGG CCCTCAGGGT CCTAGAGGAA TTCAGGGAAC ACTTGGATCC
    ATGGGAAAAT CTGGAAAAAG GGGACGCTCT GGAGCTGATG GAGCCCGTGG AATGCCAGGA
    GAGCCTGGGT CCAAGGGTGA CAGAGGTTTC GATGGTCTTC CAGGTCTCCC CGGGGAGAAG
    GGACACAGGG GTGACAAAGG TCCTCATGGG CCACCAGGGC CTCCTGGTGA AGATGGAGAG
    AGGGGAGAAG ATGGAGAAGT TGGACCAAGA GGGCTTCCCG GTGAACCTGG TGAAAGAGGA
    CTATTAGGAC CAAGGGGACC ACCAGGACCT TCAGGACAGC CTGGTGTTGC TGGTGTGGAT
    GGACCTCAAG GCCCCAAAGG AAACATGGGC CCTCAAGGAG AGCCTGGCCC TGCAGGTCAA
    CAAGGAATTC CAGGCCCCCA AGGTCTCCCT GGTCCACAAG GCTCCATCGG TCCACCTGGT
    GAGAAGGGTC CACAAGGAAA GCCTGGTTTG CCTGGATTAG CTGGAGCTGA CGGACCCCCT
    GGACATCCTG GTAAAGAAGG CCCCCCAGGA GAGAAGGGAG CATTGGGTCC CACTGGTCCC
    CAAGGTCCGA TTGGTTACCC TGGACCTCGC GGTGTTAAGG GAGCAGATGG TGTCCGAGGA
    TTGAAAGGAG GCAAAGGAGA AAAGGGTGAA GATGGATTTC CAGGATTTAA GGGTGACATG
    GGTCTTAAAG GAGACAGAGG AGAAGTTGGT CCTCTAGGTC CTCGGGGAGA AGATGGCCCA
    GAAGGTCCAA AGGGAAGATC TGGTCCATCA GGAGAATCTG GCCCAACTGG TCTTGCTGGT
    GAAAAGGGTA AACTTGGAGT CCCAGGATTG CCCGGATACC CAGGTCGACA GGGTCCCAAG
    GGTTCTACAG GTTTCCCTGG ATTTGCAGGA GCCAACGGCG AGAAAGGAGC TCGGGGTGTA
    GCTGGCAAAC CAGGTCCAAG GGGACAACGA GGTCCAACGG GTCCACGTGG TGCTAGAGGT
    CCAAGAGGTC CTACAGGAAA ACCAGGTCCT AAGGGCACAG CAGGCAATGA TGGTCCACCT
    GGCCCACCTG GTGAAAGAGG ACCTCAAGGA CCACAGGGCC CAGTTGGGTT CCCAGGACCG
    AAGGGCCCCC CTGGACCTCC TGGAAAGGAT GGATTGCCTG GCCACCCTGG ACAGCGAGGA
    GAAACAGGTT TCCAAGGAAA AACTGGGCCT CCTGGTCCTG GAGGTGTAGT AGGTCCACAG
    GGTCCCACCG GAGAAACTGG TCCCATTGGT GAAAGAGGGC ATCCTGGCCC ACCAGGTCCC
    CCTGGAGAGC AAGGTTTACC TGGATCAGCC GGAAAAGAGG GTGCTAAGGG AGATCCTGGT
    CCTTCAGGTG TGACTGGAAA AGATGGTCCC TCAGGTCTTC GTGGCTTCCC TGGTGAGAGA
    GGCCTACCTG GAGCTTCGGG TGCTGCAGGT TTGAAGGGTG GAGAAGGACC CCAGGGCCCA
    CCTGGTCCAC TTGGATCACC AGGTGAGAGA GGAGCTGCCG GCGCTGCTGG ACCAATTGGT
    CTGCCAGGAA GACCAGGACC TCAAGGCCCA CCAGGACCAG CAGGAGAGAA GGGGGCTCCA
    GGAGAGAAAG GACCTCAGGG ACCAGCAGGA CGTGATGGAA TACAAGGCCC AGTGGGACTT
    CCAGGACCTG CAGGTCCCCA AGGACCACCT GGGGAAGATG GTGACAAGGG TGAAATTGGA
    GAACCAGGAC AGAAAGGAGG AAAGGGTGAC AAAGGAGAAC AGGGTCCTCC TGGACCAGGA
    GGTCTACAAG GTCCTGTTGG AGCACCAGGT CCAGCGGGTT CTGATGGAGA GCCAGGTCCT
    AGAGGTCAAC AGGGAATGTT TGGGCAAAAG GGAGATGAAG GTTCTCGTGG ATTCCCAGGT
    CCACCTGGCC CAATTGGTCT CCAAGGACTT CCTGGACCCC CAGGTGAAAA GGGTGAAAAT
    GGAGATGTTG GTCCTATGGG TCCACCCGGT CCCCCTGGTC CTAGAGGTCC TCAAGGCCCT
    AATGGAGCTG ATGGTCCTCA AGGTCCTCCT GGATCTATTG GATCTGTTGG TCCAGTTGGT
    GAAAAGGGTG AACCAGGAGA AGCTGGAAAC CCAGGTCCCC CAGGAGAAGC TGGTGTTGGC
    GGTCCTAAAG GTGAAAGGGG AGAGAAAGGA GAGGCTGGAC CCCCAGGAGC TGCAGGACCT
    GCTGGTCCCA AGGGACCTCC AGGAGATGAT GGTCCTAAGG GAAATCCCGG TCCTGTTGGA
    TTCCCAGGAG ATCCTGGTCC ACCTGGAGAA CCTGGTCCAA GTGGACAAGA TGGTGTTTCT
    GGTGATAAAG GAGAGGATGG AGAATCAGGA CAGCCTGGTC CACCTGGTCC CTCTGGAGAA
    GCTGGACCAC CAGGACCTCC AGGAAAGAGG GGTCCTCCTG GTGCTAATGG TGCAGAAGGA
    AGACAAGGAG AGAAAGGTGC AAAGGGTGAG GCTGGAGCAG AAGGAGCACC TGGTAAGACT
    GGTCCTGTTG GACCACAAGG ACCTGCTGGG AAACCAGGAC CTGAAGGACT TAGAGGAATT
    CCAGGGCCTG TGGGAGAACA AGGACTTCCA GGTGCCCCTG GGCAGGATGG ACCTCCAGGT
    CCAATGGGTC CTCCCGGTTT ACCAGGTCTA AAAGGAGATC CCGGTACTAA GGGTGAGAAG
    GGACATCCAG GTCTAATTGG TCTGATTGGG CCACCAGGAG AGCAGGGTGA AAAGGGTGAT
    AGAGGACTTC CCGGTTTACA AGGTTCTCAC GGAACCAAAG GAGATATTGG TATATCAGGC
    CCTGCTGGTC CCACAGGTCC ACCTGGACCC CCTGGTTTAC CCGGTCCTCA AGGTCCGAAG
    GGTTCAAAGG GTTCATCTGG TCCAGCTGGT CAGAAGGGAG ATGGTGGTCT TCCTGGACCT
    CCTGGTCCAC CTGGCCCTCC TGGGGAAGTG ATCCAACCAT TACCTATCCA GTCACCAAAG
    AAGACCCGCA GATCTTCAGA TGATATGATG GCAGATGCTG GAGACAACAA CTTGGAGTAT
    GCAGATGGAA TGGAGGAGAT TTTTGGATCC CTCACTTCAT TAAAGCAAGA TATCGAGAGC
    ATGAAATTCC CCATAGGAAG TCAGAGCAAC CCAGCAAGGA CATGCAAAGA CCTGCAGCTC
    TGTCACCCAG AACTCCCTGA TGGTGAATAC TGGATTGATC CAAACCAGGG CTGCTCTGGT
    GATTCATTCA AGGTCTACTG CAATTTCACA GCTGGAGGAG AGACTTGTAT AAATCCAGAC
    AAGAAATCAG AAGGAGTGCG CATATCATCC TGGCCCAAAG AAAACCCTGG CTCATGGTTC
    AGTGAGTTCA AGAGAGGAAA GCTGCTTTCT TATGTGGACA TTCAGGGTAA CGCTATTAAC
    CAAGTACAAA TGACCTTCCT AAAACTCCTC AGTCAATCTG CCCGGCAAAA CTTCACCTAT
    ACTTGTCAAC AATCTGTTGC TTGGTATGAC AGTGTGGGCA AGCACTATGA CAAGGCCCTA
    AGGTTCCTTG GATCCAACGA CGAGGAAATT TCTTATGACA ACAGCCCATA CATTAGACCT
    TTGCATGATG GCTGTGCGCT CAAGAAAGGA TTTGCACGAA CAGTCCTTGA AATTAATACC
    CTGAAGATAG ATCAGCTGCC CATTGCAGAT GTAATGATCA GTGACTTTGG GGATCAGAAC
    CAGAAATTTG GATTTGAGGT CGGACCGGTC TGCTTCTTAG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756885.1
    CDS300..5624
    Translation

    Target ORF information:

    RefSeq Version XM_031901025.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGTAA AGGCCAATGT GGTTGACTAC
    AATCAATACA GTGAAGGAGA CTATAGTTAT CAGACAGATA CTCCTGCCCT TACTACAGGA
    CCCAATGAGG TGACCAATTA TGGTGGGTAT GGCGAGAAAG GCCAGAAAGG AGAGCCAGCA
    GTTATTGAGC CGGGAATGCT GATTGAAGGC CCCCCTGGTT TGGAGGGACC TGCTGGTCTT
    TCTGGTCCAC CTGGTTTGCA AGGACCCCCT GGTATTGCTG GAGATCCTGG TGAGAGAGGT
    CCACCTGGTC GCGCAGGGTT ACCGGGTGCT GATGGGTTAC CAGGGCCTCC AGGCACCATG
    CTGATGTTAC CGTTCCGATA TGGTGGTGAT GGAGAAAAAG GACCAACAAT CTCAGCCCAA
    GAAGCACAGG CTCAGGCCAT TCTTCAACAA GCTCGGATTG CCATGCGTGG ACCACCAGGC
    CCAATGGGAC TTACTGGAAG ATCTGGCCCT GTGGGAGCAC CTGGAGCTGC AGGAATTAAG
    GGAGACCCAG GAGACCCAGG CCCTCAGGGT CCTAGAGGAA TTCAGGGAAC ACTTGGATCC
    ATGGGAAAAT CTGGAAAAAG GGGACGCTCT GGAGCTGATG GAGCCCGTGG AATGCCAGGA
    GAGCCTGGGT CCAAGGGTGA CAGAGGTTTC GATGGTCTTC CAGGTCTCCC CGGGGAGAAG
    GGACACAGGG GTGACAAAGG TCCTCATGGG CCACCAGGGC CTCCTGGTGA AGATGGAGAG
    AGGGGAGAAG ATGGAGAAGT TGGACCAAGA GGGCTTCCCG GTGAACCTGG TGAAAGAGGA
    CTATTAGGAC CAAGGGGACC ACCAGGACCT TCAGGACAGC CTGGTGTTGC TGGTGTGGAT
    GGACCTCAAG GCCCCAAAGG AAACATGGGC CCTCAAGGAG AGCCTGGCCC TGCAGGTCAA
    CAAGGAATTC CAGGCCCCCA AGGTCTCCCT GGTCCACAAG GCTCCATCGG TCCACCTGGT
    GAGAAGGGTC CACAAGGAAA GCCTGGTTTG CCTGGATTAG CTGGAGCTGA CGGACCCCCT
    GGACATCCTG GTAAAGAAGG CCCCCCAGGA GAGAAGGGAG CATTGGGTCC CACTGGTCCC
    CAAGGTCCGA TTGGTTACCC TGGACCTCGC GGTGTTAAGG GAGCAGATGG TGTCCGAGGA
    TTGAAAGGAG GCAAAGGAGA AAAGGGTGAA GATGGATTTC CAGGATTTAA GGGTGACATG
    GGTCTTAAAG GAGACAGAGG AGAAGTTGGT CCTCTAGGTC CTCGGGGAGA AGATGGCCCA
    GAAGGTCCAA AGGGAAGATC TGGTCCATCA GGAGAATCTG GCCCAACTGG TCTTGCTGGT
    GAAAAGGGTA AACTTGGAGT CCCAGGATTG CCCGGATACC CAGGTCGACA GGGTCCCAAG
    GGTTCTACAG GTTTCCCTGG ATTTGCAGGA GCCAACGGCG AGAAAGGAGC TCGGGGTGTA
    GCTGGCAAAC CAGGTCCAAG GGGACAACGA GGTCCAACGG GTCCACGTGG TGCTAGAGGT
    CCAAGAGGTC CTACAGGAAA ACCAGGTCCT AAGGGCACAG CAGGCAATGA TGGTCCACCT
    GGCCCACCTG GTGAAAGAGG ACCTCAAGGA CCACAGGGCC CAGTTGGGTT CCCAGGACCG
    AAGGGCCCCC CTGGACCTCC TGGAAAGGAT GGATTGCCTG GCCACCCTGG ACAGCGAGGA
    GAAACAGGTT TCCAAGGAAA AACTGGGCCT CCTGGTCCTG GAGGTGTAGT AGGTCCACAG
    GGTCCCACCG GAGAAACTGG TCCCATTGGT GAAAGAGGGC ATCCTGGCCC ACCAGGTCCC
    CCTGGAGAGC AAGGTTTACC TGGATCAGCC GGAAAAGAGG GTGCTAAGGG AGATCCTGGT
    CCTTCAGGTG TGACTGGAAA AGATGGTCCC TCAGGTCTTC GTGGCTTCCC TGGTGAGAGA
    GGCCTACCTG GAGCTTCGGG TGCTGCAGGT TTGAAGGGTG GAGAAGGACC CCAGGGCCCA
    CCTGGTCCAC TTGGATCACC AGGTGAGAGA GGAGCTGCCG GCGCTGCTGG ACCAATTGGT
    CTGCCAGGAA GACCAGGACC TCAAGGCCCA CCAGGACCAG CAGGAGAGAA GGGGGCTCCA
    GGAGAGAAAG GACCTCAGGG ACCAGCAGGA CGTGATGGAA TACAAGGCCC AGTGGGACTT
    CCAGGACCTG CAGGTCCCCA AGGACCACCT GGGGAAGATG GTGACAAGGG TGAAATTGGA
    GAACCAGGAC AGAAAGGAGG AAAGGGTGAC AAAGGAGAAC AGGGTCCTCC TGGACCAGGA
    GGTCTACAAG GTCCTGTTGG AGCACCAGGT CCAGCGGGTT CTGATGGAGA GCCAGGTCCT
    AGAGGTCAAC AGGGAATGTT TGGGCAAAAG GGAGATGAAG GTTCTCGTGG ATTCCCAGGT
    CCACCTGGCC CAATTGGTCT CCAAGGACTT CCTGGACCCC CAGGTGAAAA GGGTGAAAAT
    GGAGATGTTG GTCCTATGGG TCCACCCGGT CCCCCTGGTC CTAGAGGTCC TCAAGGCCCT
    AATGGAGCTG ATGGTCCTCA AGGTCCTCCT GGATCTATTG GATCTGTTGG TCCAGTTGGT
    GAAAAGGGTG AACCAGGAGA AGCTGGAAAC CCAGGTCCCC CAGGAGAAGC TGGTGTTGGC
    GGTCCTAAAG GTGAAAGGGG AGAGAAAGGA GAGGCTGGAC CCCCAGGAGC TGCAGGACCT
    GCTGGTCCCA AGGGACCTCC AGGAGATGAT GGTCCTAAGG GAAATCCCGG TCCTGTTGGA
    TTCCCAGGAG ATCCTGGTCC ACCTGGAGAA CCTGGTCCAA GTGGACAAGA TGGTGTTTCT
    GGTGATAAAG GAGAGGATGG AGAATCAGGA CAGCCTGGTC CACCTGGTCC CTCTGGAGAA
    GCTGGACCAC CAGGACCTCC AGGAAAGAGG GGTCCTCCTG GTGCTAATGG TGCAGAAGGA
    AGACAAGGAG AGAAAGGTGC AAAGGGTGAG GCTGGAGCAG AAGGAGCACC TGGTAAGACT
    GGTCCTGTTG GACCACAAGG ACCTGCTGGG AAACCAGGAC CTGAAGGACT TAGAGGAATT
    CCAGGGCCTG TGGGAGAACA AGGACTTCCA GGTGCCCCTG GGCAGGATGG ACCTCCAGGT
    CCAATGGGTC CTCCCGGTTT ACCAGGTCTA AAAGGAGATC CCGGTACTAA GGGTGAGAAG
    GGACATCCAG GTCTAATTGG TCTGATTGGG CCACCAGGAG AGCAGGGTGA AAAGGGTGAT
    AGAGGACTTC CCGGTTTACA AGGTTCTCAC GGAACCAAAG GAGATATTGG TATATCAGGC
    CCTGCTGGTC CCACAGGTCC ACCTGGACCC CCTGGTTTAC CCGGTCCTCA AGGTCCGAAG
    GGTTCAAAGG GTTCATCTGG TCCAGCTGGT CAGAAGGGAG ATGGTGGTCT TCCTGGACCT
    CCTGGTCCAC CTGGCCCTCC TGGGGAAGTG ATCCAACCAT TACCTATCCA GTCACCAAAG
    AAGACCCGCA GATCTTCAGA TGATATGATG GCAGATGCTG GAGACAACAA CTTGGAGTAT
    GCAGATGGAA TGGAGGAGAT TTTTGGATCC CTCACTTCAT TAAAGCAAGA TATCGAGAGC
    ATGAAATTCC CCATAGGAAG TCAGAGCAAC CCAGCAAGGA CATGCAAAGA CCTGCAGCTC
    TGTCACCCAG AACTCCCTGA TGGTGAATAC TGGATTGATC CAAACCAGGG CTGCTCTGGT
    GATTCATTCA AGGTCTACTG CAATTTCACA GCTGGAGGAG AGACTTGTAT AAATCCAGAC
    AAGAAATCAG AAGGAGTGCG CATATCATCC TGGCCCAAAG AAAACCCTGG CTCATGGTTC
    AGTGAGTTCA AGAGAGGAAA GCTGCTTTCT TATGTGGACA TTCAGGGTAA CGCTATTAAC
    CAAGTACAAA TGACCTTCCT AAAACTCCTC AGTCAATCTG CCCGGCAAAA CTTCACCTAT
    ACTTGTCAAC AATCTGTTGC TTGGTATGAC AGTGTGGGCA AGCACTATGA CAAGGCCCTA
    AGGTTCCTTG GATCCAACGA CGAGGAAATT TCTTATGACA ACAGCCCATA CATTAGACCT
    TTGCATGATG GCTGTGCGCT CAAGAAAGGA TTTGCACGAA CAGTCCTTGA AATTAATACC
    CTGAAGATAG ATCAGCTGCC CATTGCAGAT GTAATGATCA GTGACTTTGG GGATCAGAAC
    CAGAAATTTG GATTTGAGGT CGGACCGGTC TGCTTCTTAG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57312
    Clone ID Related Accession (Same CDS sequence) XM_031901026.1
    Accession Version XM_031901026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGG TGACCAATTA TGGTGGGTAT GGCGAGAAAG GCCAGAAAGG AGAGCCAGCA
    GTTATTGAGC CGGGAATGCT GATTGAAGGC CCCCCTGGTT TGGAGGGACC TGCTGGTCTT
    TCTGGTCCAC CTGGTTTGCA AGGACCCCCT GGTATTGCTG GAGATCCTGG TGAGAGAGGT
    CCACCTGGTC GCGCAGGGTT ACCGGGTGCT GATGGGTTAC CAGGGCCTCC AGGCACCATG
    CTGATGTTAC CGTTCCGATA TGGTGGTGAT GGAGAAAAAG GACCAACAAT CTCAGCCCAA
    GAAGCACAGG CTCAGGCCAT TCTTCAACAA GCTCGGATTG CCATGCGTGG ACCACCAGGC
    CCAATGGGAC TTACTGGAAG ATCTGGCCCT GTGGGAGCAC CTGGAGCTGC AGGAATTAAG
    GGAGACCCAG GAGACCCAGG CCCTCAGGGT CCTAGAGGAA TTCAGGGAAC ACTTGGATCC
    ATGGGAAAAT CTGGAAAAAG GGGACGCTCT GGAGCTGATG GAGCCCGTGG AATGCCAGGA
    GAGCCTGGGT CCAAGGGTGA CAGAGGTTTC GATGGTCTTC CAGGTCTCCC CGGGGAGAAG
    GGACACAGGG GTGACAAAGG TCCTCATGGG CCACCAGGGC CTCCTGGTGA AGATGGAGAG
    AGGGGAGAAG ATGGAGAAGT TGGACCAAGA GGGCTTCCCG GTGAACCTGG TGAAAGAGGA
    CTATTAGGAC CAAGGGGACC ACCAGGACCT TCAGGACAGC CTGGTGTTGC TGGTGTGGAT
    GGACCTCAAG GCCCCAAAGG AAACATGGGC CCTCAAGGAG AGCCTGGCCC TGCAGGTCAA
    CAAGGAATTC CAGGCCCCCA AGGTCTCCCT GGTCCACAAG GCTCCATCGG TCCACCTGGT
    GAGAAGGGTC CACAAGGAAA GCCTGGTTTG CCTGGATTAG CTGGAGCTGA CGGACCCCCT
    GGACATCCTG GTAAAGAAGG CCCCCCAGGA GAGAAGGGAG CATTGGGTCC CACTGGTCCC
    CAAGGTCCGA TTGGTTACCC TGGACCTCGC GGTGTTAAGG GAGCAGATGG TGTCCGAGGA
    TTGAAAGGAG GCAAAGGAGA AAAGGGTGAA GATGGATTTC CAGGATTTAA GGGTGACATG
    GGTCTTAAAG GAGACAGAGG AGAAGTTGGT CCTCTAGGTC CTCGGGGAGA AGATGGCCCA
    GAAGGTCCAA AGGGAAGATC TGGTCCATCA GGAGAATCTG GCCCAACTGG TCTTGCTGGT
    GAAAAGGGTA AACTTGGAGT CCCAGGATTG CCCGGATACC CAGGTCGACA GGGTCCCAAG
    GGTTCTACAG GTTTCCCTGG ATTTGCAGGA GCCAACGGCG AGAAAGGAGC TCGGGGTGTA
    GCTGGCAAAC CAGGTCCAAG GGGACAACGA GGTCCAACGG GTCCACGTGG TGCTAGAGGT
    CCAAGAGGTC CTACAGGAAA ACCAGGTCCT AAGGGCACAG CAGGCAATGA TGGTCCACCT
    GGCCCACCTG GTGAAAGAGG ACCTCAAGGA CCACAGGGCC CAGTTGGGTT CCCAGGACCG
    AAGGGCCCCC CTGGACCTCC TGGAAAGGAT GGATTGCCTG GCCACCCTGG ACAGCGAGGA
    GAAACAGGTT TCCAAGGAAA AACTGGGCCT CCTGGTCCTG GAGGTGTAGT AGGTCCACAG
    GGTCCCACCG GAGAAACTGG TCCCATTGGT GAAAGAGGGC ATCCTGGCCC ACCAGGTCCC
    CCTGGAGAGC AAGGTTTACC TGGATCAGCC GGAAAAGAGG GTGCTAAGGG AGATCCTGGT
    CCTTCAGGTG TGACTGGAAA AGATGGTCCC TCAGGTCTTC GTGGCTTCCC TGGTGAGAGA
    GGCCTACCTG GAGCTTCGGG TGCTGCAGGT TTGAAGGGTG GAGAAGGACC CCAGGGCCCA
    CCTGGTCCAC TTGGATCACC AGGTGAGAGA GGAGCTGCCG GCGCTGCTGG ACCAATTGGT
    CTGCCAGGAA GACCAGGACC TCAAGGCCCA CCAGGACCAG CAGGAGAGAA GGGGGCTCCA
    GGAGAGAAAG GACCTCAGGG ACCAGCAGGA CGTGATGGAA TACAAGGCCC AGTGGGACTT
    CCAGGACCTG CAGGTCCCCA AGGACCACCT GGGGAAGATG GTGACAAGGG TGAAATTGGA
    GAACCAGGAC AGAAAGGAGG AAAGGGTGAC AAAGGAGAAC AGGGTCCTCC TGGACCAGGA
    GGTCTACAAG GTCCTGTTGG AGCACCAGGT CCAGCGGGTT CTGATGGAGA GCCAGGTCCT
    AGAGGTCAAC AGGGAATGTT TGGGCAAAAG GGAGATGAAG GTTCTCGTGG ATTCCCAGGT
    CCACCTGGCC CAATTGGTCT CCAAGGACTT CCTGGACCCC CAGGTGAAAA GGGTGAAAAT
    GGAGATGTTG GTCCTATGGG TCCACCCGGT CCCCCTGGTC CTAGAGGTCC TCAAGGCCCT
    AATGGAGCTG ATGGTCCTCA AGGTCCTCCT GGATCTATTG GATCTGTTGG TCCAGTTGGT
    GAAAAGGGTG AACCAGGAGA AGCTGGAAAC CCAGGTCCCC CAGGAGAAGC TGGTGTTGGC
    GGTCCTAAAG GTGAAAGGGG AGAGAAAGGA GAGGCTGGAC CCCCAGGAGC TGCAGGACCT
    GCTGGTCCCA AGGGACCTCC AGGAGATGAT GGTCCTAAGG GAAATCCCGG TCCTGTTGGA
    TTCCCAGGAG ATCCTGGTCC ACCTGGAGAA CCTGGTCCAA GTGGACAAGA TGGTGTTTCT
    GGTGATAAAG GAGAGGATGG AGAATCAGGA CAGCCTGGTC CACCTGGTCC CTCTGGAGAA
    GCTGGACCAC CAGGACCTCC AGGAAAGAGG GGTCCTCCTG GTGCTAATGG TGCAGAAGGA
    AGACAAGGAG AGAAAGGTGC AAAGGGTGAG GCTGGAGCAG AAGGAGCACC TGGTAAGACT
    GGTCCTGTTG GACCACAAGG ACCTGCTGGG AAACCAGGAC CTGAAGGACT TAGAGGAATT
    CCAGGGCCTG TGGGAGAACA AGGACTTCCA GGTGCCCCTG GGCAGGATGG ACCTCCAGGT
    CCAATGGGTC CTCCCGGTTT ACCAGGTCTA AAAGGAGATC CCGGTACTAA GGGTGAGAAG
    GGACATCCAG GTCTAATTGG TCTGATTGGG CCACCAGGAG AGCAGGGTGA AAAGGGTGAT
    AGAGGACTTC CCGGTTTACA AGGTTCTCAC GGAACCAAAG GAGATATTGG TATATCAGGC
    CCTGCTGGTC CCACAGGTCC ACCTGGACCC CCTGGTTTAC CCGGTCCTCA AGGTCCGAAG
    GGTTCAAAGG GTTCATCTGG TCCAGCTGGT CAGAAGGGAG ATGGTGGTCT TCCTGGACCT
    CCTGGTCCAC CTGGCCCTCC TGGGGAAGTG ATCCAACCAT TACCTATCCA GTCACCAAAG
    AAGACCCGCA GATCTTCAGA TGATATGATG GCAGATGCTG GAGACAACAA CTTGGAGTAT
    GCAGATGGAA TGGAGGAGAT TTTTGGATCC CTCACTTCAT TAAAGCAAGA TATCGAGAGC
    ATGAAATTCC CCATAGGAAG TCAGAGCAAC CCAGCAAGGA CATGCAAAGA CCTGCAGCTC
    TGTCACCCAG AACTCCCTGA TGGTGAATAC TGGATTGATC CAAACCAGGG CTGCTCTGGT
    GATTCATTCA AGGTCTACTG CAATTTCACA GCTGGAGGAG AGACTTGTAT AAATCCAGAC
    AAGAAATCAG AAGGAGTGCG CATATCATCC TGGCCCAAAG AAAACCCTGG CTCATGGTTC
    AGTGAGTTCA AGAGAGGAAA GCTGCTTTCT TATGTGGACA TTCAGGGTAA CGCTATTAAC
    CAAGTACAAA TGACCTTCCT AAAACTCCTC AGTCAATCTG CCCGGCAAAA CTTCACCTAT
    ACTTGTCAAC AATCTGTTGC TTGGTATGAC AGTGTGGGCA AGCACTATGA CAAGGCCCTA
    AGGTTCCTTG GATCCAACGA CGAGGAAATT TCTTATGACA ACAGCCCATA CATTAGACCT
    TTGCATGATG GCTGTGCGCT CAAGAAAGGA TTTGCACGAA CAGTCCTTGA AATTAATACC
    CTGAAGATAG ATCAGCTGCC CATTGCAGAT GTAATGATCA GTGACTTTGG GGATCAGAAC
    CAGAAATTTG GATTTGAGGT CGGACCGGTC TGCTTCTTAG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031756886.1
    CDS298..5262
    Translation

    Target ORF information:

    RefSeq Version XM_031901026.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGG TGACCAATTA TGGTGGGTAT GGCGAGAAAG GCCAGAAAGG AGAGCCAGCA
    GTTATTGAGC CGGGAATGCT GATTGAAGGC CCCCCTGGTT TGGAGGGACC TGCTGGTCTT
    TCTGGTCCAC CTGGTTTGCA AGGACCCCCT GGTATTGCTG GAGATCCTGG TGAGAGAGGT
    CCACCTGGTC GCGCAGGGTT ACCGGGTGCT GATGGGTTAC CAGGGCCTCC AGGCACCATG
    CTGATGTTAC CGTTCCGATA TGGTGGTGAT GGAGAAAAAG GACCAACAAT CTCAGCCCAA
    GAAGCACAGG CTCAGGCCAT TCTTCAACAA GCTCGGATTG CCATGCGTGG ACCACCAGGC
    CCAATGGGAC TTACTGGAAG ATCTGGCCCT GTGGGAGCAC CTGGAGCTGC AGGAATTAAG
    GGAGACCCAG GAGACCCAGG CCCTCAGGGT CCTAGAGGAA TTCAGGGAAC ACTTGGATCC
    ATGGGAAAAT CTGGAAAAAG GGGACGCTCT GGAGCTGATG GAGCCCGTGG AATGCCAGGA
    GAGCCTGGGT CCAAGGGTGA CAGAGGTTTC GATGGTCTTC CAGGTCTCCC CGGGGAGAAG
    GGACACAGGG GTGACAAAGG TCCTCATGGG CCACCAGGGC CTCCTGGTGA AGATGGAGAG
    AGGGGAGAAG ATGGAGAAGT TGGACCAAGA GGGCTTCCCG GTGAACCTGG TGAAAGAGGA
    CTATTAGGAC CAAGGGGACC ACCAGGACCT TCAGGACAGC CTGGTGTTGC TGGTGTGGAT
    GGACCTCAAG GCCCCAAAGG AAACATGGGC CCTCAAGGAG AGCCTGGCCC TGCAGGTCAA
    CAAGGAATTC CAGGCCCCCA AGGTCTCCCT GGTCCACAAG GCTCCATCGG TCCACCTGGT
    GAGAAGGGTC CACAAGGAAA GCCTGGTTTG CCTGGATTAG CTGGAGCTGA CGGACCCCCT
    GGACATCCTG GTAAAGAAGG CCCCCCAGGA GAGAAGGGAG CATTGGGTCC CACTGGTCCC
    CAAGGTCCGA TTGGTTACCC TGGACCTCGC GGTGTTAAGG GAGCAGATGG TGTCCGAGGA
    TTGAAAGGAG GCAAAGGAGA AAAGGGTGAA GATGGATTTC CAGGATTTAA GGGTGACATG
    GGTCTTAAAG GAGACAGAGG AGAAGTTGGT CCTCTAGGTC CTCGGGGAGA AGATGGCCCA
    GAAGGTCCAA AGGGAAGATC TGGTCCATCA GGAGAATCTG GCCCAACTGG TCTTGCTGGT
    GAAAAGGGTA AACTTGGAGT CCCAGGATTG CCCGGATACC CAGGTCGACA GGGTCCCAAG
    GGTTCTACAG GTTTCCCTGG ATTTGCAGGA GCCAACGGCG AGAAAGGAGC TCGGGGTGTA
    GCTGGCAAAC CAGGTCCAAG GGGACAACGA GGTCCAACGG GTCCACGTGG TGCTAGAGGT
    CCAAGAGGTC CTACAGGAAA ACCAGGTCCT AAGGGCACAG CAGGCAATGA TGGTCCACCT
    GGCCCACCTG GTGAAAGAGG ACCTCAAGGA CCACAGGGCC CAGTTGGGTT CCCAGGACCG
    AAGGGCCCCC CTGGACCTCC TGGAAAGGAT GGATTGCCTG GCCACCCTGG ACAGCGAGGA
    GAAACAGGTT TCCAAGGAAA AACTGGGCCT CCTGGTCCTG GAGGTGTAGT AGGTCCACAG
    GGTCCCACCG GAGAAACTGG TCCCATTGGT GAAAGAGGGC ATCCTGGCCC ACCAGGTCCC
    CCTGGAGAGC AAGGTTTACC TGGATCAGCC GGAAAAGAGG GTGCTAAGGG AGATCCTGGT
    CCTTCAGGTG TGACTGGAAA AGATGGTCCC TCAGGTCTTC GTGGCTTCCC TGGTGAGAGA
    GGCCTACCTG GAGCTTCGGG TGCTGCAGGT TTGAAGGGTG GAGAAGGACC CCAGGGCCCA
    CCTGGTCCAC TTGGATCACC AGGTGAGAGA GGAGCTGCCG GCGCTGCTGG ACCAATTGGT
    CTGCCAGGAA GACCAGGACC TCAAGGCCCA CCAGGACCAG CAGGAGAGAA GGGGGCTCCA
    GGAGAGAAAG GACCTCAGGG ACCAGCAGGA CGTGATGGAA TACAAGGCCC AGTGGGACTT
    CCAGGACCTG CAGGTCCCCA AGGACCACCT GGGGAAGATG GTGACAAGGG TGAAATTGGA
    GAACCAGGAC AGAAAGGAGG AAAGGGTGAC AAAGGAGAAC AGGGTCCTCC TGGACCAGGA
    GGTCTACAAG GTCCTGTTGG AGCACCAGGT CCAGCGGGTT CTGATGGAGA GCCAGGTCCT
    AGAGGTCAAC AGGGAATGTT TGGGCAAAAG GGAGATGAAG GTTCTCGTGG ATTCCCAGGT
    CCACCTGGCC CAATTGGTCT CCAAGGACTT CCTGGACCCC CAGGTGAAAA GGGTGAAAAT
    GGAGATGTTG GTCCTATGGG TCCACCCGGT CCCCCTGGTC CTAGAGGTCC TCAAGGCCCT
    AATGGAGCTG ATGGTCCTCA AGGTCCTCCT GGATCTATTG GATCTGTTGG TCCAGTTGGT
    GAAAAGGGTG AACCAGGAGA AGCTGGAAAC CCAGGTCCCC CAGGAGAAGC TGGTGTTGGC
    GGTCCTAAAG GTGAAAGGGG AGAGAAAGGA GAGGCTGGAC CCCCAGGAGC TGCAGGACCT
    GCTGGTCCCA AGGGACCTCC AGGAGATGAT GGTCCTAAGG GAAATCCCGG TCCTGTTGGA
    TTCCCAGGAG ATCCTGGTCC ACCTGGAGAA CCTGGTCCAA GTGGACAAGA TGGTGTTTCT
    GGTGATAAAG GAGAGGATGG AGAATCAGGA CAGCCTGGTC CACCTGGTCC CTCTGGAGAA
    GCTGGACCAC CAGGACCTCC AGGAAAGAGG GGTCCTCCTG GTGCTAATGG TGCAGAAGGA
    AGACAAGGAG AGAAAGGTGC AAAGGGTGAG GCTGGAGCAG AAGGAGCACC TGGTAAGACT
    GGTCCTGTTG GACCACAAGG ACCTGCTGGG AAACCAGGAC CTGAAGGACT TAGAGGAATT
    CCAGGGCCTG TGGGAGAACA AGGACTTCCA GGTGCCCCTG GGCAGGATGG ACCTCCAGGT
    CCAATGGGTC CTCCCGGTTT ACCAGGTCTA AAAGGAGATC CCGGTACTAA GGGTGAGAAG
    GGACATCCAG GTCTAATTGG TCTGATTGGG CCACCAGGAG AGCAGGGTGA AAAGGGTGAT
    AGAGGACTTC CCGGTTTACA AGGTTCTCAC GGAACCAAAG GAGATATTGG TATATCAGGC
    CCTGCTGGTC CCACAGGTCC ACCTGGACCC CCTGGTTTAC CCGGTCCTCA AGGTCCGAAG
    GGTTCAAAGG GTTCATCTGG TCCAGCTGGT CAGAAGGGAG ATGGTGGTCT TCCTGGACCT
    CCTGGTCCAC CTGGCCCTCC TGGGGAAGTG ATCCAACCAT TACCTATCCA GTCACCAAAG
    AAGACCCGCA GATCTTCAGA TGATATGATG GCAGATGCTG GAGACAACAA CTTGGAGTAT
    GCAGATGGAA TGGAGGAGAT TTTTGGATCC CTCACTTCAT TAAAGCAAGA TATCGAGAGC
    ATGAAATTCC CCATAGGAAG TCAGAGCAAC CCAGCAAGGA CATGCAAAGA CCTGCAGCTC
    TGTCACCCAG AACTCCCTGA TGGTGAATAC TGGATTGATC CAAACCAGGG CTGCTCTGGT
    GATTCATTCA AGGTCTACTG CAATTTCACA GCTGGAGGAG AGACTTGTAT AAATCCAGAC
    AAGAAATCAG AAGGAGTGCG CATATCATCC TGGCCCAAAG AAAACCCTGG CTCATGGTTC
    AGTGAGTTCA AGAGAGGAAA GCTGCTTTCT TATGTGGACA TTCAGGGTAA CGCTATTAAC
    CAAGTACAAA TGACCTTCCT AAAACTCCTC AGTCAATCTG CCCGGCAAAA CTTCACCTAT
    ACTTGTCAAC AATCTGTTGC TTGGTATGAC AGTGTGGGCA AGCACTATGA CAAGGCCCTA
    AGGTTCCTTG GATCCAACGA CGAGGAAATT TCTTATGACA ACAGCCCATA CATTAGACCT
    TTGCATGATG GCTGTGCGCT CAAGAAAGGA TTTGCACGAA CAGTCCTTGA AATTAATACC
    CTGAAGATAG ATCAGCTGCC CATTGCAGAT GTAATGATCA GTGACTTTGG GGATCAGAAC
    CAGAAATTTG GATTTGAGGT CGGACCGGTC TGCTTCTTAG GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa09043
    Clone ID Related Accession (Same CDS sequence) XM_004913713.2
    Accession Version XM_004913713.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGCCA ATGTGGTTGA CTACAATCAA
    TACAGTGAAG GAGACTATAG TTATCAGACA GATACTCCTG CCCTTACTAC AGGACCCAAT
    GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG GAGGAATCCA TTGTGGAGGA ATCCATTGTG
    GAGGACCCAT TTGTGGAGGA GTACGTAACA GGAGAGGACT ATGAATCAGA GAAAAAGCTG
    GTTGAGGAAA CAGAGTATGG GGTAAAAACT GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA
    GGAGAAGGTT TAGGAGAATA TGATATTTAT GACTATAAGG AGTATGAAGA GAAGCTCCCC
    AACCCTACCA CACAAGAGGT CGGCCCTGGT GTGCCTGCTG AAACTGATAT CTCTGAAACC
    AGTGTGACCA ATTATGGTGG GTATGGCGAG AAAGGCCAGA AAGGAGAGCC AGCAGTTATT
    GAGCCGGGAA TGCTGATTGA AGGCCCCCCT GGTTTGGAGG GACCTGCTGG TCTTTCTGGT
    CCACCTGGTT TGCAAGGACC CCCTGGTATT GCTGGAGATC CTGGTGAGAG AGGTCCACCT
    GGTCGCGCAG GGTTACCGGG TGCTGATGGG TTACCAGGGC CTCCAGGCAC CATGCTGATG
    TTACCGTTCC GATATGGTGG TGATGGAGAA AAAGGACCAA CAATCTCAGC CCAAGAAGCA
    CAGGCTCAGG CCATTCTTCA ACAAGCTCGG ATTGCCATGC GTGGACCACC AGGCCCAATG
    GGACTTACTG GAAGATCTGG CCCTGTGGGA GCACCTGGAG CTGCAGGAAT TAAGGGAGAC
    CCAGGAGACC CAGGCCCTCA GGGTCCTAGA GGAATTCAGG GAACACTTGG ATCCATGGGA
    AAATCTGGAA AAAGGGGACG CTCTGGAGCT GATGGAGCCC GTGGAATGCC AGGAGAGCCT
    GGGTCCAAGG GTGACAGAGG TTTCGATGGT CTTCCAGGTC TCCCCGGGGA GAAGGGACAC
    AGGGGTGACA AAGGTCCTCA TGGGCCACCA GGGCCTCCTG GTGAAGATGG AGAGAGGGGA
    GAAGATGGAG AAGTTGGACC AAGAGGGCTT CCCGGTGAAC CTGGTGAAAG AGGACTATTA
    GGACCAAGGG GACCACCAGG ACCTTCAGGA CAGCCTGGTG TTGCTGGTGT GGATGGACCT
    CAAGGCCCCA AAGGAAACAT GGGCCCTCAA GGAGAGCCTG GCCCTGCAGG TCAACAAGGA
    ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA CAAGGCTCCA TCGGTCCACC TGGTGAGAAG
    GGTCCACAAG GAAAGCCTGG TTTGCCTGGA TTAGCTGGAG CTGATGGACC CCCTGGACAT
    CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG GGAGCATTGG GTCCCACTGG TCCCCAAGGT
    CCGATTGGTT ACCCTGGACC TCGCGGTGTT AAGGGAGCAG ATGGTGTCCG AGGATTGAAA
    GGAGGCAAAG GAGAAAAGGG TGAAGATGGA TTTCCAGGAT TTAAGGGTGA CATGGGTCTT
    AAAGGAGACA GAGGAGAAGT TGGTCCTCTA GGTCCTCGGG GAGAAGATGG CCCAGAAGGT
    CCAAAGGGAA GATCTGGTCC ATCAGGAGAA TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG
    GGTAAACTTG GAGTCCCAGG ATTGCCCGGA TACCCAGGTC GACAGGGTCC CAAGGGTTCT
    ACAGGTTTCC CTGGATTTGC AGGAGCCAAC GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC
    AAACCAGGTC CAAGGGGACA ACGAGGTCCA ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA
    GGTCCTACAG GAAAACCAGG TCCTAAGGGC ACAGCAGGCA ATGATGGTCC ACCTGGCCCA
    CCTGGTGAAA GAGGACCTCA AGGACCACAG GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC
    CCCCCTGGAC CTCCTGGAAA GGATGGATTG CCTGGCCACC CTGGACAGCG AGGAGAAACA
    GGTTTCCAAG GAAAAACTGG GCCTCCTGGT CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC
    ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA GGGCATCCTG GCCCACCAGG TCCCCCTGGA
    GAGCAAGGTT TACCTGGATC AGCCGGAAAA GAGGGTGCTA AGGGAGATCC TGGTCCTTCA
    GGTGTGACTG GAAAAGATGG TCCCTCAGGT CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA
    CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG GGTGGAGAAG GACCCCAGGG CCCACCTGGT
    CCACTTGGAT CACCAGGTGA GAGAGGAGCT GCCGGCGCTG CTGGACCAAT TGGTCTGCCA
    GGAAGACCAG GACCTCAAGG CCCACCAGGA CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG
    AAAGGACCTC AGGGACCAGC AGGACGTGAT GGAATACAAG GCCCAGTGGG ACTTCCAGGA
    CCTGCAGGTC CCCAAGGACC ACCTGGGGAA GATGGTGACA AGGGTGAAAT TGGAGAACCA
    GGACAGAAAG GAGGAAAGGG TGACAAAGGA GAACAGGGTC CTCCTGGACC AGGAGGTCTA
    CAAGGTCCTG TTGGAGCACC AGGTCCAGCG GGTTCTGATG GAGAGCCAGG TCCTAGAGGT
    CAACAGGGAA TGTTTGGGCA AAAGGGAGAT GAAGGTTCTC GTGGATTCCC AGGTCCACCT
    GGCCCAATTG GTCTCCAAGG ACTTCCTGGA CCCCCAGGTG AAAAGGGTGA AAATGGAGAT
    GTTGGTCCTA TGGGTCCACC CGGTCCCCCT GGTCCTAGAG GTCCTCAAGG CCCTAATGGA
    GCTGATGGTC CTCAAGGTCC TCCTGGATCT ATTGGATCTG TTGGTCCAGT TGGTGAAAAG
    GGTGAACCAG GAGAAGCTGG AAACCCAGGT CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT
    AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT GGACCCCCAG GAGCTGCAGG ACCTGCTGGT
    CCCAAGGGAC CTCCAGGAGA TGATGGTCCT AAGGGAAATC CCGGTCCTGT TGGATTCCCA
    GGAGATCCTG GTCCACCTGG AGAACCTGGT CCAAGTGGAC AAGATGGTGT TTCTGGTGAT
    AAAGGAGAGG ATGGAGAATC AGGACAGCCT GGTCCACCTG GTCCCTCTGG AGAAGCTGGA
    CCACCAGGAC CTCCAGGAAA GAGGGGTCCT CCTGGTGCTA ATGGTGCAGA AGGAAGACAA
    GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA GCAGAAGGAG CACCTGGTAA GACTGGTCCT
    GTTGGACCAC AAGGACCTGC TGGGAAACCA GGACCTGAAG GACTTAGAGG AATTCCAGGG
    CCTGTGGGAG AACAAGGACT TCCAGGTGCC CCTGGGCAGG ATGGACCTCC AGGTCCAATG
    GGTCCTCCCG GTTTACCAGG TCTAAAAGGA GATCCCGGTA CTAAGGGTGA GAAGGGACAT
    CCAGGTCTAA TTGGTCTGAT TGGGCCACCA GGAGAGCAGG GTGAAAAGGG TGATAGAGGA
    CTTCCCGGTT TACAAGGTTC TCACGGAACC AAAGGAGATA TTGGTATATC AGGCCCTGCT
    GGTCCCACAG GTCCACCTGG ACCCCCTGGT TTACCCGGTC CTCAAGGTCC GAAGGGTTCA
    AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG GGAGATGGTG GTCTTCCTGG ACCTCCTGGT
    CCACCTGGCC CTCCTGGGGA AGTGATCCAA CCATTACCTA TCCAGTCACC AAAGAAGACC
    CGCAGATCTT CAGATGATAT GATGGCAGAT GCTGGAGACA ACAACTTGGA GTATGCAGAT
    GGAATGGAGG AGATTTTTGG ATCCCTCACT TCATTAAAGC AAGATATCGA GAGCATGAAA
    TTCCCCATAG GAAGTCAGAG CAACCCAGCA AGGACATGCA AAGACCTGCA GCTCTGTCAC
    CCAGAACTCC CTGATGGTGA ATACTGGATT GATCCAAACC AGGGCTGCTC TGGTGATTCA
    TTCAAGGTCT ACTGCAATTT CACAGCTGGA GGAGAGACTT GTATAAATCC AGACAAGAAA
    TCAGAAGGAG TGCGCATATC ATCCTGGCCC AAAGAAAACC CTGGCTCATG GTTCAGTGAG
    TTCAAGAGAG GAAAGCTGCT TTCTTATGTG GACATTCAGG GTAACGCTAT TAACCAAGTA
    CAAATGACCT TCCTAAAACT CCTCAGTCAA TCTGCCCGGC AAAACTTCAC CTATACTTGT
    CAACAATCTG TTGCTTGGTA TGACAGTGTG GGCAAGCACT ATGACAAGGC CCTAAGGTTC
    CTTGGATCCA ACGACGAGGA AATTTCTTAT GACAACAGCC CATACATTAG ACCTTTGCAT
    GATGGCTGTG CGCTCAAGAA AGGATTTGCA CGAACAGTCC TTGAAATTAA TACCCTGAAG
    ATAGATCAGC TGCCCATTGC AGATGTAATG ATCAGTGACT TTGGGGATCA GAACCAGAAA
    TTTGGATTTG AGGTCGGACC GGTCTGCTTC TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913770.1
    CDS333..5951
    Translation

    Target ORF information:

    RefSeq Version XM_004913713.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913713.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGAAGA AGAAAGCCAG TTCCAAATCG AAGAAGAAGC CAGTGCCCCC TAAATCAAAG
    GACAAACCCC AAAAGGTCAC GCCCAAAAAC TCATTTGCAG CTAAGAAGAA GAAGGGCAAG
    ACCGATTACC AGGCGTCAGC AAGAAAAAGA ATAGGGGCCA ATGTGGTTGA CTACAATCAA
    TACAGTGAAG GAGACTATAG TTATCAGACA GATACTCCTG CCCTTACTAC AGGACCCAAT
    GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG GAGGAATCCA TTGTGGAGGA ATCCATTGTG
    GAGGACCCAT TTGTGGAGGA GTACGTAACA GGAGAGGACT ATGAATCAGA GAAAAAGCTG
    GTTGAGGAAA CAGAGTATGG GGTAAAAACT GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA
    GGAGAAGGTT TAGGAGAATA TGATATTTAT GACTATAAGG AGTATGAAGA GAAGCTCCCC
    AACCCTACCA CACAAGAGGT CGGCCCTGGT GTGCCTGCTG AAACTGATAT CTCTGAAACC
    AGTGTGACCA ATTATGGTGG GTATGGCGAG AAAGGCCAGA AAGGAGAGCC AGCAGTTATT
    GAGCCGGGAA TGCTGATTGA AGGCCCCCCT GGTTTGGAGG GACCTGCTGG TCTTTCTGGT
    CCACCTGGTT TGCAAGGACC CCCTGGTATT GCTGGAGATC CTGGTGAGAG AGGTCCACCT
    GGTCGCGCAG GGTTACCGGG TGCTGATGGG TTACCAGGGC CTCCAGGCAC CATGCTGATG
    TTACCGTTCC GATATGGTGG TGATGGAGAA AAAGGACCAA CAATCTCAGC CCAAGAAGCA
    CAGGCTCAGG CCATTCTTCA ACAAGCTCGG ATTGCCATGC GTGGACCACC AGGCCCAATG
    GGACTTACTG GAAGATCTGG CCCTGTGGGA GCACCTGGAG CTGCAGGAAT TAAGGGAGAC
    CCAGGAGACC CAGGCCCTCA GGGTCCTAGA GGAATTCAGG GAACACTTGG ATCCATGGGA
    AAATCTGGAA AAAGGGGACG CTCTGGAGCT GATGGAGCCC GTGGAATGCC AGGAGAGCCT
    GGGTCCAAGG GTGACAGAGG TTTCGATGGT CTTCCAGGTC TCCCCGGGGA GAAGGGACAC
    AGGGGTGACA AAGGTCCTCA TGGGCCACCA GGGCCTCCTG GTGAAGATGG AGAGAGGGGA
    GAAGATGGAG AAGTTGGACC AAGAGGGCTT CCCGGTGAAC CTGGTGAAAG AGGACTATTA
    GGACCAAGGG GACCACCAGG ACCTTCAGGA CAGCCTGGTG TTGCTGGTGT GGATGGACCT
    CAAGGCCCCA AAGGAAACAT GGGCCCTCAA GGAGAGCCTG GCCCTGCAGG TCAACAAGGA
    ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA CAAGGCTCCA TCGGTCCACC TGGTGAGAAG
    GGTCCACAAG GAAAGCCTGG TTTGCCTGGA TTAGCTGGAG CTGATGGACC CCCTGGACAT
    CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG GGAGCATTGG GTCCCACTGG TCCCCAAGGT
    CCGATTGGTT ACCCTGGACC TCGCGGTGTT AAGGGAGCAG ATGGTGTCCG AGGATTGAAA
    GGAGGCAAAG GAGAAAAGGG TGAAGATGGA TTTCCAGGAT TTAAGGGTGA CATGGGTCTT
    AAAGGAGACA GAGGAGAAGT TGGTCCTCTA GGTCCTCGGG GAGAAGATGG CCCAGAAGGT
    CCAAAGGGAA GATCTGGTCC ATCAGGAGAA TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG
    GGTAAACTTG GAGTCCCAGG ATTGCCCGGA TACCCAGGTC GACAGGGTCC CAAGGGTTCT
    ACAGGTTTCC CTGGATTTGC AGGAGCCAAC GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC
    AAACCAGGTC CAAGGGGACA ACGAGGTCCA ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA
    GGTCCTACAG GAAAACCAGG TCCTAAGGGC ACAGCAGGCA ATGATGGTCC ACCTGGCCCA
    CCTGGTGAAA GAGGACCTCA AGGACCACAG GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC
    CCCCCTGGAC CTCCTGGAAA GGATGGATTG CCTGGCCACC CTGGACAGCG AGGAGAAACA
    GGTTTCCAAG GAAAAACTGG GCCTCCTGGT CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC
    ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA GGGCATCCTG GCCCACCAGG TCCCCCTGGA
    GAGCAAGGTT TACCTGGATC AGCCGGAAAA GAGGGTGCTA AGGGAGATCC TGGTCCTTCA
    GGTGTGACTG GAAAAGATGG TCCCTCAGGT CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA
    CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG GGTGGAGAAG GACCCCAGGG CCCACCTGGT
    CCACTTGGAT CACCAGGTGA GAGAGGAGCT GCCGGCGCTG CTGGACCAAT TGGTCTGCCA
    GGAAGACCAG GACCTCAAGG CCCACCAGGA CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG
    AAAGGACCTC AGGGACCAGC AGGACGTGAT GGAATACAAG GCCCAGTGGG ACTTCCAGGA
    CCTGCAGGTC CCCAAGGACC ACCTGGGGAA GATGGTGACA AGGGTGAAAT TGGAGAACCA
    GGACAGAAAG GAGGAAAGGG TGACAAAGGA GAACAGGGTC CTCCTGGACC AGGAGGTCTA
    CAAGGTCCTG TTGGAGCACC AGGTCCAGCG GGTTCTGATG GAGAGCCAGG TCCTAGAGGT
    CAACAGGGAA TGTTTGGGCA AAAGGGAGAT GAAGGTTCTC GTGGATTCCC AGGTCCACCT
    GGCCCAATTG GTCTCCAAGG ACTTCCTGGA CCCCCAGGTG AAAAGGGTGA AAATGGAGAT
    GTTGGTCCTA TGGGTCCACC CGGTCCCCCT GGTCCTAGAG GTCCTCAAGG CCCTAATGGA
    GCTGATGGTC CTCAAGGTCC TCCTGGATCT ATTGGATCTG TTGGTCCAGT TGGTGAAAAG
    GGTGAACCAG GAGAAGCTGG AAACCCAGGT CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT
    AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT GGACCCCCAG GAGCTGCAGG ACCTGCTGGT
    CCCAAGGGAC CTCCAGGAGA TGATGGTCCT AAGGGAAATC CCGGTCCTGT TGGATTCCCA
    GGAGATCCTG GTCCACCTGG AGAACCTGGT CCAAGTGGAC AAGATGGTGT TTCTGGTGAT
    AAAGGAGAGG ATGGAGAATC AGGACAGCCT GGTCCACCTG GTCCCTCTGG AGAAGCTGGA
    CCACCAGGAC CTCCAGGAAA GAGGGGTCCT CCTGGTGCTA ATGGTGCAGA AGGAAGACAA
    GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA GCAGAAGGAG CACCTGGTAA GACTGGTCCT
    GTTGGACCAC AAGGACCTGC TGGGAAACCA GGACCTGAAG GACTTAGAGG AATTCCAGGG
    CCTGTGGGAG AACAAGGACT TCCAGGTGCC CCTGGGCAGG ATGGACCTCC AGGTCCAATG
    GGTCCTCCCG GTTTACCAGG TCTAAAAGGA GATCCCGGTA CTAAGGGTGA GAAGGGACAT
    CCAGGTCTAA TTGGTCTGAT TGGGCCACCA GGAGAGCAGG GTGAAAAGGG TGATAGAGGA
    CTTCCCGGTT TACAAGGTTC TCACGGAACC AAAGGAGATA TTGGTATATC AGGCCCTGCT
    GGTCCCACAG GTCCACCTGG ACCCCCTGGT TTACCCGGTC CTCAAGGTCC GAAGGGTTCA
    AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG GGAGATGGTG GTCTTCCTGG ACCTCCTGGT
    CCACCTGGCC CTCCTGGGGA AGTGATCCAA CCATTACCTA TCCAGTCACC AAAGAAGACC
    CGCAGATCTT CAGATGATAT GATGGCAGAT GCTGGAGACA ACAACTTGGA GTATGCAGAT
    GGAATGGAGG AGATTTTTGG ATCCCTCACT TCATTAAAGC AAGATATCGA GAGCATGAAA
    TTCCCCATAG GAAGTCAGAG CAACCCAGCA AGGACATGCA AAGACCTGCA GCTCTGTCAC
    CCAGAACTCC CTGATGGTGA ATACTGGATT GATCCAAACC AGGGCTGCTC TGGTGATTCA
    TTCAAGGTCT ACTGCAATTT CACAGCTGGA GGAGAGACTT GTATAAATCC AGACAAGAAA
    TCAGAAGGAG TGCGCATATC ATCCTGGCCC AAAGAAAACC CTGGCTCATG GTTCAGTGAG
    TTCAAGAGAG GAAAGCTGCT TTCTTATGTG GACATTCAGG GTAACGCTAT TAACCAAGTA
    CAAATGACCT TCCTAAAACT CCTCAGTCAA TCTGCCCGGC AAAACTTCAC CTATACTTGT
    CAACAATCTG TTGCTTGGTA TGACAGTGTG GGCAAGCACT ATGACAAGGC CCTAAGGTTC
    CTTGGATCCA ACGACGAGGA AATTTCTTAT GACAACAGCC CATACATTAG ACCTTTGCAT
    GATGGCTGTG CGCTCAAGAA AGGATTTGCA CGAACAGTCC TTGAAATTAA TACCCTGAAG
    ATAGATCAGC TGCCCATTGC AGATGTAATG ATCAGTGACT TTGGGGATCA GAACCAGAAA
    TTTGGATTTG AGGTCGGACC GGTCTGCTTC TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa09044
    Clone ID Related Accession (Same CDS sequence) XM_004913714.3
    Accession Version XM_004913714.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XI) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGGCCA ATGTGGTTGA CTACAATCAA TACAGTGAAG GAGACTATAG TTATCAGACA
    GATACTCCTG CCCTTACTAC AGGACCCAAT GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG
    GAGGAATCCA TTGTGGAGGA ATCCATTGTG GAGGACCCAT TTGTGGAGGA GTACGTAACA
    GGAGAGGACT ATGAATCAGA GAAAAAGCTG GTTGAGGAAA CAGAGTATGG GGTAAAAACT
    GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA GGAGAAGGTT TAGGAGAATA TGATATTTAT
    GACTATAAGG AGTATGAAGA GAAGCTCCCC AACCCTACCA CACAAGAGGT CGGCCCTGGT
    GTGCCTGCTG AAACTGATAT CTCTGAAACC AGTGTGACCA ATTATGGTGG GTATGGCGAG
    AAAGGCCAGA AAGGAGAGCC AGCAGTTATT GAGCCGGGAA TGCTGATTGA AGGCCCCCCT
    GGTTTGGAGG GACCTGCTGG TCTTTCTGGT CCACCTGGTT TGCAAGGACC CCCTGGTATT
    GCTGGAGATC CTGGTGAGAG AGGTCCACCT GGTCGCGCAG GGTTACCGGG TGCTGATGGG
    TTACCAGGGC CTCCAGGCAC CATGCTGATG TTACCGTTCC GATATGGTGG TGATGGAGAA
    AAAGGACCAA CAATCTCAGC CCAAGAAGCA CAGGCTCAGG CCATTCTTCA ACAAGCTCGG
    ATTGCCATGC GTGGACCACC AGGCCCAATG GGACTTACTG GAAGATCTGG CCCTGTGGGA
    GCACCTGGAG CTGCAGGAAT TAAGGGAGAC CCAGGAGACC CAGGCCCTCA GGGTCCTAGA
    GGAATTCAGG GAACACTTGG ATCCATGGGA AAATCTGGAA AAAGGGGACG CTCTGGAGCT
    GATGGAGCCC GTGGAATGCC AGGAGAGCCT GGGTCCAAGG GTGACAGAGG TTTCGATGGT
    CTTCCAGGTC TCCCCGGGGA GAAGGGACAC AGGGGTGACA AAGGTCCTCA TGGGCCACCA
    GGGCCTCCTG GTGAAGATGG AGAGAGGGGA GAAGATGGAG AAGTTGGACC AAGAGGGCTT
    CCCGGTGAAC CTGGTGAAAG AGGACTATTA GGACCAAGGG GACCACCAGG ACCTTCAGGA
    CAGCCTGGTG TTGCTGGTGT GGATGGACCT CAAGGCCCCA AAGGAAACAT GGGCCCTCAA
    GGAGAGCCTG GCCCTGCAGG TCAACAAGGA ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA
    CAAGGCTCCA TCGGTCCACC TGGTGAGAAG GGTCCACAAG GAAAGCCTGG TTTGCCTGGA
    TTAGCTGGAG CTGATGGACC CCCTGGACAT CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG
    GGAGCATTGG GTCCCACTGG TCCCCAAGGT CCGATTGGTT ACCCTGGACC TCGCGGTGTT
    AAGGGAGCAG ATGGTGTCCG AGGATTGAAA GGAGGCAAAG GAGAAAAGGG TGAAGATGGA
    TTTCCAGGAT TTAAGGGTGA CATGGGTCTT AAAGGAGACA GAGGAGAAGT TGGTCCTCTA
    GGTCCTCGGG GAGAAGATGG CCCAGAAGGT CCAAAGGGAA GATCTGGTCC ATCAGGAGAA
    TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG GGTAAACTTG GAGTCCCAGG ATTGCCCGGA
    TACCCAGGTC GACAGGGTCC CAAGGGTTCT ACAGGTTTCC CTGGATTTGC AGGAGCCAAC
    GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC AAACCAGGTC CAAGGGGACA ACGAGGTCCA
    ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA GGTCCTACAG GAAAACCAGG TCCTAAGGGC
    ACAGCAGGCA ATGATGGTCC ACCTGGCCCA CCTGGTGAAA GAGGACCTCA AGGACCACAG
    GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC CCCCCTGGAC CTCCTGGAAA GGATGGATTG
    CCTGGCCACC CTGGACAGCG AGGAGAAACA GGTTTCCAAG GAAAAACTGG GCCTCCTGGT
    CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA
    GGGCATCCTG GCCCACCAGG TCCCCCTGGA GAGCAAGGTT TACCTGGATC AGCCGGAAAA
    GAGGGTGCTA AGGGAGATCC TGGTCCTTCA GGTGTGACTG GAAAAGATGG TCCCTCAGGT
    CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG
    GGTGGAGAAG GACCCCAGGG CCCACCTGGT CCACTTGGAT CACCAGGTGA GAGAGGAGCT
    GCCGGCGCTG CTGGACCAAT TGGTCTGCCA GGAAGACCAG GACCTCAAGG CCCACCAGGA
    CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG AAAGGACCTC AGGGACCAGC AGGACGTGAT
    GGAATACAAG GCCCAGTGGG ACTTCCAGGA CCTGCAGGTC CCCAAGGACC ACCTGGGGAA
    GATGGTGACA AGGGTGAAAT TGGAGAACCA GGACAGAAAG GAGGAAAGGG TGACAAAGGA
    GAACAGGGTC CTCCTGGACC AGGAGGTCTA CAAGGTCCTG TTGGAGCACC AGGTCCAGCG
    GGTTCTGATG GAGAGCCAGG TCCTAGAGGT CAACAGGGAA TGTTTGGGCA AAAGGGAGAT
    GAAGGTTCTC GTGGATTCCC AGGTCCACCT GGCCCAATTG GTCTCCAAGG ACTTCCTGGA
    CCCCCAGGTG AAAAGGGTGA AAATGGAGAT GTTGGTCCTA TGGGTCCACC CGGTCCCCCT
    GGTCCTAGAG GTCCTCAAGG CCCTAATGGA GCTGATGGTC CTCAAGGTCC TCCTGGATCT
    ATTGGATCTG TTGGTCCAGT TGGTGAAAAG GGTGAACCAG GAGAAGCTGG AAACCCAGGT
    CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT
    GGACCCCCAG GAGCTGCAGG ACCTGCTGGT CCCAAGGGAC CTCCAGGAGA TGATGGTCCT
    AAGGGAAATC CCGGTCCTGT TGGATTCCCA GGAGATCCTG GTCCACCTGG AGAACCTGGT
    CCAAGTGGAC AAGATGGTGT TTCTGGTGAT AAAGGAGAGG ATGGAGAATC AGGACAGCCT
    GGTCCACCTG GTCCCTCTGG AGAAGCTGGA CCACCAGGAC CTCCAGGAAA GAGGGGTCCT
    CCTGGTGCTA ATGGTGCAGA AGGAAGACAA GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA
    GCAGAAGGAG CACCTGGTAA GACTGGTCCT GTTGGACCAC AAGGACCTGC TGGGAAACCA
    GGACCTGAAG GACTTAGAGG AATTCCAGGG CCTGTGGGAG AACAAGGACT TCCAGGTGCC
    CCTGGGCAGG ATGGACCTCC AGGTCCAATG GGTCCTCCCG GTTTACCAGG TCTAAAAGGA
    GATCCCGGTA CTAAGGGTGA GAAGGGACAT CCAGGTCTAA TTGGTCTGAT TGGGCCACCA
    GGAGAGCAGG GTGAAAAGGG TGATAGAGGA CTTCCCGGTT TACAAGGTTC TCACGGAACC
    AAAGGAGATA TTGGTATATC AGGCCCTGCT GGTCCCACAG GTCCACCTGG ACCCCCTGGT
    TTACCCGGTC CTCAAGGTCC GAAGGGTTCA AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG
    GGAGATGGTG GTCTTCCTGG ACCTCCTGGT CCACCTGGCC CTCCTGGGGA AGTGATCCAA
    CCATTACCTA TCCAGTCACC AAAGAAGACC CGCAGATCTT CAGATGATAT GATGGCAGAT
    GCTGGAGACA ACAACTTGGA GTATGCAGAT GGAATGGAGG AGATTTTTGG ATCCCTCACT
    TCATTAAAGC AAGATATCGA GAGCATGAAA TTCCCCATAG GAAGTCAGAG CAACCCAGCA
    AGGACATGCA AAGACCTGCA GCTCTGTCAC CCAGAACTCC CTGATGGTGA ATACTGGATT
    GATCCAAACC AGGGCTGCTC TGGTGATTCA TTCAAGGTCT ACTGCAATTT CACAGCTGGA
    GGAGAGACTT GTATAAATCC AGACAAGAAA TCAGAAGGAG TGCGCATATC ATCCTGGCCC
    AAAGAAAACC CTGGCTCATG GTTCAGTGAG TTCAAGAGAG GAAAGCTGCT TTCTTATGTG
    GACATTCAGG GTAACGCTAT TAACCAAGTA CAAATGACCT TCCTAAAACT CCTCAGTCAA
    TCTGCCCGGC AAAACTTCAC CTATACTTGT CAACAATCTG TTGCTTGGTA TGACAGTGTG
    GGCAAGCACT ATGACAAGGC CCTAAGGTTC CTTGGATCCA ACGACGAGGA AATTTCTTAT
    GACAACAGCC CATACATTAG ACCTTTGCAT GATGGCTGTG CGCTCAAGAA AGGATTTGCA
    CGAACAGTCC TTGAAATTAA TACCCTGAAG ATAGATCAGC TGCCCATTGC AGATGTAATG
    ATCAGTGACT TTGGGGATCA GAACCAGAAA TTTGGATTTG AGGTCGGACC GGTCTGCTTC
    TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913771.1
    CDS330..5798
    Translation

    Target ORF information:

    RefSeq Version XM_004913714.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type V, alpha 3 (col5a3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913714.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGAGGT GGAGCACAAG GTGGAAAACG AAAAGGAGGT TGTGGGATTC ATCCCTCAGC 
    TCCCTAGTTC TCATCACTTT GACTTTCCTT TATCAAGCTG CAGAGGTCAG AGGAGCTGGA
    CCTGTTGATG TACTCAAATC ATTAGAATTT CACAGTTTAC CAGAAGGTGT ATCGAAAACA
    GCAGGATTTT GCACAAACAG AAGGGCAGCC AAAGGATCAG ATACAGCATT TAGAGTTCAG
    AAACATGCCC AGCTTAGCAT CCCAACAAAG AAATTATTCC CAGGGGGAGT ATTCCCAAAA
    GATTTCTCAG TCCTGCTGAC AGTCAAGCCC AAGAAGGGTC TGCAGTCGTT CCTCCTGTCC
    ATTTACAATG ATCAAAAAGT ACAGCAACTT GGAGTGGAGA TTGGAAGTTC CCCTATCTTC
    CAATATGAAG ATCATAATGG AAAACCTGCC CCTAAGGATT ACCCCATGTT TAGCTCTGTT
    AACATTGCAG ATGGCAAATG GCACCGTGTT GCAATCAGTG TGGAAAAGAA GACAGTCACA
    ATTATCGTAG ACTGCAAGAA GAAAGCCACA TTACCCCTCC CAAGGAGTGA TAAGCCTATA
    GTGTACACCA ATGGAATCGT TGTGTTTGGA ACCAGCTTGT TGGAAGAAGA CGTGTTTGAG
    GGTGACATCC AGCAGTTATT GATTAGTGAT GACTCCAAAG CTGCATATGA CTACTGTGAA
    CATTTCATGC CGGACTGTGA CGCTCCAGTC CCCAACCTAC CGCAAGCCCA AGAACCACAG
    GTGGATGAGT ATGCGCCTGA AGAGCTAATC GAGTATGACT ATGAGTATGG GGACCCAGAT
    TACAAGGAGA CTGAGCTTGT AACAGAGGCA CCCTCTCTGA CTGAAGAACT TATAGCTCAG
    ACAGAGGCCA ATGTGGTTGA CTACAATCAA TACAGTGAAG GAGACTATAG TTATCAGACA
    GATACTCCTG CCCTTACTAC AGGACCCAAT GAGCAAAAAC CAGAGGAGGA GTCTTTGGTG
    GAGGAATCCA TTGTGGAGGA ATCCATTGTG GAGGACCCAT TTGTGGAGGA GTACGTAACA
    GGAGAGGACT ATGAATCAGA GAAAAAGCTG GTTGAGGAAA CAGAGTATGG GGTAAAAACT
    GTTAGCCCCA CAGAGTCTGA GCCAGTAGTA GGAGAAGGTT TAGGAGAATA TGATATTTAT
    GACTATAAGG AGTATGAAGA GAAGCTCCCC AACCCTACCA CACAAGAGGT CGGCCCTGGT
    GTGCCTGCTG AAACTGATAT CTCTGAAACC AGTGTGACCA ATTATGGTGG GTATGGCGAG
    AAAGGCCAGA AAGGAGAGCC AGCAGTTATT GAGCCGGGAA TGCTGATTGA AGGCCCCCCT
    GGTTTGGAGG GACCTGCTGG TCTTTCTGGT CCACCTGGTT TGCAAGGACC CCCTGGTATT
    GCTGGAGATC CTGGTGAGAG AGGTCCACCT GGTCGCGCAG GGTTACCGGG TGCTGATGGG
    TTACCAGGGC CTCCAGGCAC CATGCTGATG TTACCGTTCC GATATGGTGG TGATGGAGAA
    AAAGGACCAA CAATCTCAGC CCAAGAAGCA CAGGCTCAGG CCATTCTTCA ACAAGCTCGG
    ATTGCCATGC GTGGACCACC AGGCCCAATG GGACTTACTG GAAGATCTGG CCCTGTGGGA
    GCACCTGGAG CTGCAGGAAT TAAGGGAGAC CCAGGAGACC CAGGCCCTCA GGGTCCTAGA
    GGAATTCAGG GAACACTTGG ATCCATGGGA AAATCTGGAA AAAGGGGACG CTCTGGAGCT
    GATGGAGCCC GTGGAATGCC AGGAGAGCCT GGGTCCAAGG GTGACAGAGG TTTCGATGGT
    CTTCCAGGTC TCCCCGGGGA GAAGGGACAC AGGGGTGACA AAGGTCCTCA TGGGCCACCA
    GGGCCTCCTG GTGAAGATGG AGAGAGGGGA GAAGATGGAG AAGTTGGACC AAGAGGGCTT
    CCCGGTGAAC CTGGTGAAAG AGGACTATTA GGACCAAGGG GACCACCAGG ACCTTCAGGA
    CAGCCTGGTG TTGCTGGTGT GGATGGACCT CAAGGCCCCA AAGGAAACAT GGGCCCTCAA
    GGAGAGCCTG GCCCTGCAGG TCAACAAGGA ATTCCAGGCC CCCAAGGTCT CCCTGGTCCA
    CAAGGCTCCA TCGGTCCACC TGGTGAGAAG GGTCCACAAG GAAAGCCTGG TTTGCCTGGA
    TTAGCTGGAG CTGATGGACC CCCTGGACAT CCTGGTAAAG AAGGCCCCCC AGGAGAGAAG
    GGAGCATTGG GTCCCACTGG TCCCCAAGGT CCGATTGGTT ACCCTGGACC TCGCGGTGTT
    AAGGGAGCAG ATGGTGTCCG AGGATTGAAA GGAGGCAAAG GAGAAAAGGG TGAAGATGGA
    TTTCCAGGAT TTAAGGGTGA CATGGGTCTT AAAGGAGACA GAGGAGAAGT TGGTCCTCTA
    GGTCCTCGGG GAGAAGATGG CCCAGAAGGT CCAAAGGGAA GATCTGGTCC ATCAGGAGAA
    TCTGGCCCAA CTGGTCTTGC TGGTGAAAAG GGTAAACTTG GAGTCCCAGG ATTGCCCGGA
    TACCCAGGTC GACAGGGTCC CAAGGGTTCT ACAGGTTTCC CTGGATTTGC AGGAGCCAAC
    GGCGAGAAAG GAGCTCGGGG TGTAGCTGGC AAACCAGGTC CAAGGGGACA ACGAGGTCCA
    ACGGGTCCAC GTGGTGCTAG AGGTCCAAGA GGTCCTACAG GAAAACCAGG TCCTAAGGGC
    ACAGCAGGCA ATGATGGTCC ACCTGGCCCA CCTGGTGAAA GAGGACCTCA AGGACCACAG
    GGCCCAGTTG GGTTCCCAGG ACCGAAGGGC CCCCCTGGAC CTCCTGGAAA GGATGGATTG
    CCTGGCCACC CTGGACAGCG AGGAGAAACA GGTTTCCAAG GAAAAACTGG GCCTCCTGGT
    CCTGGAGGTG TAGTAGGTCC ACAGGGTCCC ACCGGAGAAA CTGGTCCCAT TGGTGAAAGA
    GGGCATCCTG GCCCACCAGG TCCCCCTGGA GAGCAAGGTT TACCTGGATC AGCCGGAAAA
    GAGGGTGCTA AGGGAGATCC TGGTCCTTCA GGTGTGACTG GAAAAGATGG TCCCTCAGGT
    CTTCGTGGCT TCCCTGGTGA GAGAGGCCTA CCTGGAGCTT CGGGTGCTGC AGGTTTGAAG
    GGTGGAGAAG GACCCCAGGG CCCACCTGGT CCACTTGGAT CACCAGGTGA GAGAGGAGCT
    GCCGGCGCTG CTGGACCAAT TGGTCTGCCA GGAAGACCAG GACCTCAAGG CCCACCAGGA
    CCAGCAGGAG AGAAGGGGGC TCCAGGAGAG AAAGGACCTC AGGGACCAGC AGGACGTGAT
    GGAATACAAG GCCCAGTGGG ACTTCCAGGA CCTGCAGGTC CCCAAGGACC ACCTGGGGAA
    GATGGTGACA AGGGTGAAAT TGGAGAACCA GGACAGAAAG GAGGAAAGGG TGACAAAGGA
    GAACAGGGTC CTCCTGGACC AGGAGGTCTA CAAGGTCCTG TTGGAGCACC AGGTCCAGCG
    GGTTCTGATG GAGAGCCAGG TCCTAGAGGT CAACAGGGAA TGTTTGGGCA AAAGGGAGAT
    GAAGGTTCTC GTGGATTCCC AGGTCCACCT GGCCCAATTG GTCTCCAAGG ACTTCCTGGA
    CCCCCAGGTG AAAAGGGTGA AAATGGAGAT GTTGGTCCTA TGGGTCCACC CGGTCCCCCT
    GGTCCTAGAG GTCCTCAAGG CCCTAATGGA GCTGATGGTC CTCAAGGTCC TCCTGGATCT
    ATTGGATCTG TTGGTCCAGT TGGTGAAAAG GGTGAACCAG GAGAAGCTGG AAACCCAGGT
    CCCCCAGGAG AAGCTGGTGT TGGCGGTCCT AAAGGTGAAA GGGGAGAGAA AGGAGAGGCT
    GGACCCCCAG GAGCTGCAGG ACCTGCTGGT CCCAAGGGAC CTCCAGGAGA TGATGGTCCT
    AAGGGAAATC CCGGTCCTGT TGGATTCCCA GGAGATCCTG GTCCACCTGG AGAACCTGGT
    CCAAGTGGAC AAGATGGTGT TTCTGGTGAT AAAGGAGAGG ATGGAGAATC AGGACAGCCT
    GGTCCACCTG GTCCCTCTGG AGAAGCTGGA CCACCAGGAC CTCCAGGAAA GAGGGGTCCT
    CCTGGTGCTA ATGGTGCAGA AGGAAGACAA GGAGAGAAAG GTGCAAAGGG TGAGGCTGGA
    GCAGAAGGAG CACCTGGTAA GACTGGTCCT GTTGGACCAC AAGGACCTGC TGGGAAACCA
    GGACCTGAAG GACTTAGAGG AATTCCAGGG CCTGTGGGAG AACAAGGACT TCCAGGTGCC
    CCTGGGCAGG ATGGACCTCC AGGTCCAATG GGTCCTCCCG GTTTACCAGG TCTAAAAGGA
    GATCCCGGTA CTAAGGGTGA GAAGGGACAT CCAGGTCTAA TTGGTCTGAT TGGGCCACCA
    GGAGAGCAGG GTGAAAAGGG TGATAGAGGA CTTCCCGGTT TACAAGGTTC TCACGGAACC
    AAAGGAGATA TTGGTATATC AGGCCCTGCT GGTCCCACAG GTCCACCTGG ACCCCCTGGT
    TTACCCGGTC CTCAAGGTCC GAAGGGTTCA AAGGGTTCAT CTGGTCCAGC TGGTCAGAAG
    GGAGATGGTG GTCTTCCTGG ACCTCCTGGT CCACCTGGCC CTCCTGGGGA AGTGATCCAA
    CCATTACCTA TCCAGTCACC AAAGAAGACC CGCAGATCTT CAGATGATAT GATGGCAGAT
    GCTGGAGACA ACAACTTGGA GTATGCAGAT GGAATGGAGG AGATTTTTGG ATCCCTCACT
    TCATTAAAGC AAGATATCGA GAGCATGAAA TTCCCCATAG GAAGTCAGAG CAACCCAGCA
    AGGACATGCA AAGACCTGCA GCTCTGTCAC CCAGAACTCC CTGATGGTGA ATACTGGATT
    GATCCAAACC AGGGCTGCTC TGGTGATTCA TTCAAGGTCT ACTGCAATTT CACAGCTGGA
    GGAGAGACTT GTATAAATCC AGACAAGAAA TCAGAAGGAG TGCGCATATC ATCCTGGCCC
    AAAGAAAACC CTGGCTCATG GTTCAGTGAG TTCAAGAGAG GAAAGCTGCT TTCTTATGTG
    GACATTCAGG GTAACGCTAT TAACCAAGTA CAAATGACCT TCCTAAAACT CCTCAGTCAA
    TCTGCCCGGC AAAACTTCAC CTATACTTGT CAACAATCTG TTGCTTGGTA TGACAGTGTG
    GGCAAGCACT ATGACAAGGC CCTAAGGTTC CTTGGATCCA ACGACGAGGA AATTTCTTAT
    GACAACAGCC CATACATTAG ACCTTTGCAT GATGGCTGTG CGCTCAAGAA AGGATTTGCA
    CGAACAGTCC TTGAAATTAA TACCCTGAAG ATAGATCAGC TGCCCATTGC AGATGTAATG
    ATCAGTGACT TTGGGGATCA GAACCAGAAA TTTGGATTTG AGGTCGGACC GGTCTGCTTC
    TTAGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.