dhx8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhx8
Entrez Gene ID	733563
Full Name	DEAH-box helicase 8
Synonyms	ddx8,hrh1,prp22,prpf22
General protein information	Preferred Names DEAH-box helicase 8 Names ATP-dependent RNA helicase DHX8 DEAH (Asp-Glu-Ala-His) box polypeptide 8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004918626.3	XP_004918683.1	ATP-dependent RNA helicase DHX8 isoform X1
XM_004918626.4	XP_004918683.1	ATP-dependent RNA helicase DHX8 isoform X1
XM_002932524.4	XP_002932570.2	ATP-dependent RNA helicase DHX8 isoform X1
XM_012952725.2	XP_012808179.1	ATP-dependent RNA helicase DHX8 isoform X2
XM_018097874.1	XP_017953363.1	ATP-dependent RNA helicase DHX8 isoform X2
XM_004918627.3	XP_004918684.1	ATP-dependent RNA helicase DHX8 isoform X2
XM_004918627.4	XP_004918684.1	ATP-dependent RNA helicase DHX8 isoform X2
XM_002932524.5	XP_002932570.2	ATP-dependent RNA helicase DHX8 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX8	XP_001154202.1
Canis lupus familiaris (dog)	DHX8	XP_537627.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007212	XP_308573.4
Oryza sativa (rice)	Os06g0343100	NP_001057574.1
Homo sapiens (human)	DHX8	NP_004932.1
Danio rerio (zebrafish)	dhx8	XP_002663993.1
Saccharomyces cerevisiae (baker's yeast)	PRP22	NP_010929.3
Schizosaccharomyces pombe (fission yeast)	prp22	NP_593253.1
Xenopus tropicalis (tropical clawed frog)	LOC733563	XP_002932570.2
Bos taurus (cattle)	DHX8	NP_001193020.1
Mus musculus (house mouse)	Dhx8	NP_659080.2
Magnaporthe oryzae (rice blast fungus)	MGG_08807	XP_003719164.1
	DHX8	XP_002800602.1
Gallus gallus (chicken)	DHX8	XP_418105.3
Kluyveromyces lactis	KLLA0B11638g	XP_452048.1
Neurospora crassa	NCU06318	XP_962580.1
Rattus norvegicus (Norway rat)	Dhx8	NP_001041309.1
Caenorhabditis elegans (roundworm)	mog-5	NP_495019.1
Eremothecium gossypii	AGOS_AAR020W	NP_982561.2
Arabidopsis thaliana (thale cress)	AT3G26560	NP_189288.1

Gene Ontology

The following dhx8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhx8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa17459	XM_002932524.5 Latest version!	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa17459	XM_004918626.4 Latest version!	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa17460	XM_004918627.4 Latest version!	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa17460	XM_018097874.1 Latest version!	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa17460	XM_012952725.2 Latest version!	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa17459	XM_004918626.3	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa17460	XM_004918627.3	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa17459	XM_002932524.4	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhx8 ( XM_004918626.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918683.1 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa17459
Clone ID Related Accession (Same CDS sequence)	XM_004918626.3 , XM_004918626.4 , XM_002932524.4 , XM_002932524.5
Accession Version	XM_004918626.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918626.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918683.1
CDS	164..3709
Translation	MEDPAMDELQKLEYLSLVSKVCTELDNHLGINDKDLAEFVISIAEKATTFETFKSALEKNGAEFTDSLINNLLRLIQTMRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_004918626.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918626.4

ORF Insert Sequence:

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_002932524.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932570.2 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa17459
Clone ID Related Accession (Same CDS sequence)	XM_004918626.3 , XM_004918626.4 , XM_002932524.4 , XM_002932524.5
Accession Version	XM_002932524.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932524.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932570.2
CDS	122..3667
Translation	MEDPAMDELQKLEYLSLVSKVCTELDNHLGINDKDLAEFVISIAEKATTFETFKSALEKNGAEFTDSLINNLLRLIQTMRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_002932524.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932524.5

ORF Insert Sequence:

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_004918627.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918684.1 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X3, mRNA.

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CloneID	OXa17460
Clone ID Related Accession (Same CDS sequence)	XM_012952725.2 , XM_018097874.1 , XM_004918627.3 , XM_004918627.4
Accession Version	XM_004918627.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918627.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918684.1
CDS	1251..4562
Translation	MRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_004918627.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918627.4

ORF Insert Sequence:

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_004918626.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918683.1 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X2, mRNA.

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CloneID	OXa17459
Clone ID Related Accession (Same CDS sequence)	XM_004918626.3 , XM_004918626.4 , XM_002932524.4 , XM_002932524.5
Accession Version	XM_004918626.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918626.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918683.1
CDS	161..3706
Translation	MEDPAMDELQKLEYLSLVSKVCTELDNHLGINDKDLAEFVISIAEKATTFETFKSALEKNGAEFTDSLINNLLRLIQTMRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_004918626.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918626.3

ORF Insert Sequence:

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_002932524.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932570.2 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa17459
Clone ID Related Accession (Same CDS sequence)	XM_004918626.3 , XM_004918626.4 , XM_002932524.4 , XM_002932524.5
Accession Version	XM_002932524.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932524.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932570.2
CDS	128..3673
Translation	MEDPAMDELQKLEYLSLVSKVCTELDNHLGINDKDLAEFVISIAEKATTFETFKSALEKNGAEFTDSLINNLLRLIQTMRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_002932524.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932524.4

ORF Insert Sequence:

ATGGAAGATC CTGCAATGGA TGAGCTACAG AAGCTTGAAT ATCTGTCCCT GGTCTCCAAA 
GTCTGCACAG AGCTGGACAA TCATTTGGGT ATCAATGACA AAGATTTAGC TGAATTTGTG 
ATCAGCATTG CTGAGAAAGC CACTACTTTT GAAACCTTTA AATCTGCTCT TGAGAAAAAT 
GGGGCCGAAT TCACAGACTC CCTTATAAAC AATTTGTTGC GTCTGATTCA GACAATGAGA 
CCTCCAGCAA AACCCTCTAC AAGCAAAGAA ACAGTGATCA AGCCAAAGAA TGAAAAAGAA 
AAGCTGAAAG AGTTATTTCC CGCACTTTGC AGGGCAGATA ATCCAAGTGT CCGGACCATG 
CTAGATCCTG ATGATGTAAA AGTGGCTGCA GATGCACTGC AAGAACTTGA AGCTCTAATG 
CCCAAGGATG AGAGAAGTAG CAAACACAAA GATAAAAAAA AGAGGAGCCG CAGTCGAAGC 
CGGGAGAAAG GCAGCAAACA CAAACGTAAA TCTCGCTCTA GGTCCCAAAG CAGCAAAAAA 
ACAAAAGACA GATATAGATC ACGAAGCAGA AGCCCCATTA AGGAGCACCG TAACAACGAA 
CACCATTCTG ATAAATATGC CCCTTCTTGG AAAGAGAAGC ATGTTGATAG ACCGCCACCT 
GAAGAGCCAG CAATTGGGGA CATATATAAT GGCAAAGTGA CAAGTATCAT GCAGTTTGGC 
TGCTTTGTCC AGCTGGAAGG CTTAAGGAAA CGTTGGGAAG GATTGGTTCA CATATCGGAA 
CTGAGGAGGG AAGGACGCGT TGCGAATGTA GCAGACGTAG TAAGCAAGGG ACAAAGAGTA 
AAAGTTAAAG TGCTTTCTTT CACTGGATGC AAAACCAGCC TCAGCATGAA GGACGTTAAC 
CAAGATACTG GCGAAGACCT GAATCCCAAC AGACGCAGAA ATCTTATAGG TGACAGCAAT 
GAAGAAGCTT CCATGAGGAA CCCAGACCGA CCAAGCCATC TCTCTTTAGT AAATGCTCCA 
GAGATTGAAG ATGACACCTT AGAAAGAAAA CGCCTTACCA AAATCTCAGA TCCTGAAAAG 
TGGGAGATTA AACAGATGAT TGCAGCTAAT GTTCTATCAA AAGAAGAATT CCCTGATTTT 
GATGAGGAGA CTGGAATCCT TCCTAAGGTG GATGATGAGG AAGACGAGGA CCTGGAGATA 
GAGTTGGTTG AAGAAGAGCC TCCGTTTCTT AGAGGACACA CAAAACAGAG CATGGACATG 
AGCCCTATAA AAATAGTAAA GAATCCTGAC GGCTCCCTCT CTCAGGCTGC CATGATGCAG 
AGTGCTCTGG CAAAGGAGAG GCGAGAAATG AAGCAAGCTC AAAGAGAAGC TGAGATGGAT 
TCTATACCTA TGGGATTGAA CAAACACTGG GTAGATCCTT TGCCTGATGC TGATGGAAGA 
CAAATTGCTG CTAATATGAG AGGTATTGGG ATGATGCCAA ATGATATTCC AGAATGGAAG 
AAGCATGCTT TCGGTGGCAA CAAAGCATCC TATGGAAAAA AGACGCAGAT GTCAATTGTG 
GAACAGAGGG AAAGTCTCCC CATTTACAAG CTGAAGGAGC AGCTTGTGCA GGCAGTGCAT 
GACAACCAGA TTCTCATTGT TATCGGAGAG ACCGGCTCAG GTAAAACAAC CCAAATCACC 
CAGTATTTGG CAGAAGCTGG TTACACGACC AGGGGGAAAA TAGGCTGCAC CCAACCAAGA 
AGAGTGGCTG CAATGTCAGT TGCTAAGAGA GTGTCAGAAG AATATGGCTG CTGTTTAGGA 
CAAGAGGTTG GATACACTAT TCGATTTGAA GATTGTACAA GTCCAGAGAC AGTCATAAAG 
TACATGACTG ATGGTATGTT ACTGAGAGAG TGCCTTATAG ACCCCGACCT GACACAGTAT 
GCCATAATCA TGTTGGACGA GGCTCATGAG AGGACAATTC ATACAGATGT GTTGTTTGGT 
CTCCTTAAAA AGACTGTACA AAAGCGGCCA GATATGAAGC TCATTGTGAC CTCTGCAACA 
CTGGATGCAG TCAAATTCTC CCAGTATTTT TATGAGGCAC CAATTTTCAC CATTCCCGGT 
CGGACATACC CTGTTGAGAT TCTTTATACC AAAGAGCCAG AGACAGACTA CCTGGATGCC 
AGTCTCATTA CGGTCATGCA GATACATTTG ACAGAACCAC CGGGGGATAT TTTAGTATTC 
CTTACTGGAC AAGAGGAAAT TGATACTGCT TGTGAAATTC TTTATGAAAG AATGAAGTCT 
CTTGGACCTG ATGTGCCAGA GCTGATTATC TTACCTGTTT ATTCAGCATT GCCCAGTGAA 
ATGCAGACAA GGATATTTGA CCCAGCACCT CCTGGAAGCA GAAAGGTTGT GATTGCTACT 
AACATTGCAG AGACCTCCCT AACTATTGAT GGAATATATT ATGTGGTGGA TCCAGGATTT 
GTGAAGCAGA AAGTTTACAA CTCCAAGACA GGAATTGATC AACTCGTAGT AACACCAATT 
TCCCAGGCAC AAGCTAAACA GCGAGCTGGG AGAGCAGGAA GAACAGGACC TGGCAAGTGT 
TACCGGCTTT ATACTGAACG AGCCTATCGG GATGAAATGC TGACCACTAA TGTACCTGAG 
ATCCAAAGAA CCAACTTAGC AAGTACAGTT CTGTCACTCA AGGCTATGGG AATCAATGAT 
TTACTCTCAT TTGATTTTAT GGATGCACCC CCAATGGAGA CACTGATCAC TGCTATGGAG 
CAGCTGTACA CACTTGGTGC ACTGGATGAC GAAGGACTTC TTACACGCCT TGGCCGTAGA 
ATGGCAGAGT TTCCTCTGGA GCCAATGCTG TGTAAGATGT TGATCATGTC TGTCCATCTT 
GGCTGTAGTG AGGAGATGTT AACCATCGTC TCAATGCTTT CAGTTCAGAA TGTGTTCTAC 
AGACCCAAGG ACAAACAAGC TCTCGCAGAC CAGAAAAAGG CAAAGTTCCA CCAGACAGAG 
GGAGATCACC TGACACTACT CGCCGTTTAT AATTCCTGGA AAAACAATAA ATTTTCCAAT 
CCCTGGTGCT ATGAAAATTT CATTCAGGCT CGTTCCCTAC GCCGGGCTCA GGATATCCGG 
AAACAGATGT TGGGAATAAT GGACAGGCAT AAACTCGATG TGGTATCTTG TGGAAAAGCC 
ACTGTGAGGG TACAGAAGGC AATATGCAGT GGGTTTTTTC GAAACGCAGC AAAAAAGGAC 
CCTCAAGAGG GATATCGGAC TCTCATAGAC CAACAGGTTG TCTACATTCA TCCATCCAGC 
GCCCTTTTCA ACAGGCAGCC AGAATGGGTT GTGTATCATG AACTTGTCCT GACTACCAAA 
GAATACATGC GAGAAGTGAC CACCATAGAT CCCCGCTGGC TTGTAGAATT TGCCCCGGCC 
TTCTTTAAAG TCTCTGACCC AACTAAGCTT AGCAAGCAGA AGAAACAACA GAGGCTGGAG 
CCTCTCTACA ACAGATACGA GGAGCCCAAC GCTTGGAGAA TTTCCAGGGC ATTCAGAAGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_012952725.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808179.1 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X3, mRNA.

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CloneID	OXa17460
Clone ID Related Accession (Same CDS sequence)	XM_012952725.2 , XM_018097874.1 , XM_004918627.3 , XM_004918627.4
Accession Version	XM_012952725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808179.1
CDS	632..3943
Translation	MRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_012952725.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952725.2

ORF Insert Sequence:

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_018097874.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953363.1 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X4, mRNA.

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CloneID	OXa17460
Clone ID Related Accession (Same CDS sequence)	XM_012952725.2 , XM_018097874.1 , XM_004918627.3 , XM_004918627.4
Accession Version	XM_018097874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953363.1
CDS	61..3372
Translation	MRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_018097874.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097874.1

ORF Insert Sequence:

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx8 ( XM_004918627.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918684.1 Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X5, mRNA.

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CloneID	OXa17460
Clone ID Related Accession (Same CDS sequence)	XM_012952725.2 , XM_018097874.1 , XM_004918627.3 , XM_004918627.4
Accession Version	XM_004918627.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-dependent RNA helicase DHX8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918627.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918684.1
CDS	304..3615
Translation	MRPPAKPSTSKETVIKPKNEKEKLKELFPALCRADNPSVRTMLDPDDVKVAADALQELEALMPKDERSSKHKDKKKRSRSRSREKGSKHKRKSRSRSQSSKKTKDRYRSRSRSPIKEHRNNEHHSDKYAPSWKEKHVDRPPPEEPAIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGCKTSLSMKDVNQDTGEDLNPNRRRNLIGDSNEEASMRNPDRPSHLSLVNAPEIEDDTLERKRLTKISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERREMKQAQREAEMDSIPMGLNKHWVDPLPDADGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSIVEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTTRGKIGCTQPRRVAAMSVAKRVSEEYGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRPDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_004918627.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 8 (dhx8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918627.3

ORF Insert Sequence:

ATGAGACCTC CAGCAAAACC CTCTACAAGC AAAGAAACAG TGATCAAGCC AAAGAATGAA 
AAAGAAAAGC TGAAAGAGTT ATTTCCCGCA CTTTGCAGGG CAGATAATCC AAGTGTCCGG 
ACCATGCTAG ATCCTGATGA TGTAAAAGTG GCTGCAGATG CACTGCAAGA ACTTGAAGCT 
CTAATGCCCA AGGATGAGAG AAGTAGCAAA CACAAAGATA AAAAAAAGAG GAGCCGCAGT 
CGAAGCCGGG AGAAAGGCAG CAAACACAAA CGTAAATCTC GCTCTAGGTC CCAAAGCAGC 
AAAAAAACAA AAGACAGATA TAGATCACGA AGCAGAAGCC CCATTAAGGA GCACCGTAAC 
AACGAACACC ATTCTGATAA ATATGCCCCT TCTTGGAAAG AGAAGCATGT TGATAGACCG 
CCACCTGAAG AGCCAGCAAT TGGGGACATA TATAATGGCA AAGTGACAAG TATCATGCAG 
TTTGGCTGCT TTGTCCAGCT GGAAGGCTTA AGGAAACGTT GGGAAGGATT GGTTCACATA 
TCGGAACTGA GGAGGGAAGG ACGCGTTGCG AATGTAGCAG ACGTAGTAAG CAAGGGACAA 
AGAGTAAAAG TTAAAGTGCT TTCTTTCACT GGATGCAAAA CCAGCCTCAG CATGAAGGAC 
GTTAACCAAG ATACTGGCGA AGACCTGAAT CCCAACAGAC GCAGAAATCT TATAGGTGAC 
AGCAATGAAG AAGCTTCCAT GAGGAACCCA GACCGACCAA GCCATCTCTC TTTAGTAAAT 
GCTCCAGAGA TTGAAGATGA CACCTTAGAA AGAAAACGCC TTACCAAAAT CTCAGATCCT 
GAAAAGTGGG AGATTAAACA GATGATTGCA GCTAATGTTC TATCAAAAGA AGAATTCCCT 
GATTTTGATG AGGAGACTGG AATCCTTCCT AAGGTGGATG ATGAGGAAGA CGAGGACCTG 
GAGATAGAGT TGGTTGAAGA AGAGCCTCCG TTTCTTAGAG GACACACAAA ACAGAGCATG 
GACATGAGCC CTATAAAAAT AGTAAAGAAT CCTGACGGCT CCCTCTCTCA GGCTGCCATG 
ATGCAGAGTG CTCTGGCAAA GGAGAGGCGA GAAATGAAGC AAGCTCAAAG AGAAGCTGAG 
ATGGATTCTA TACCTATGGG ATTGAACAAA CACTGGGTAG ATCCTTTGCC TGATGCTGAT 
GGAAGACAAA TTGCTGCTAA TATGAGAGGT ATTGGGATGA TGCCAAATGA TATTCCAGAA 
TGGAAGAAGC ATGCTTTCGG TGGCAACAAA GCATCCTATG GAAAAAAGAC GCAGATGTCA 
ATTGTGGAAC AGAGGGAAAG TCTCCCCATT TACAAGCTGA AGGAGCAGCT TGTGCAGGCA 
GTGCATGACA ACCAGATTCT CATTGTTATC GGAGAGACCG GCTCAGGTAA AACAACCCAA 
ATCACCCAGT ATTTGGCAGA AGCTGGTTAC ACGACCAGGG GGAAAATAGG CTGCACCCAA 
CCAAGAAGAG TGGCTGCAAT GTCAGTTGCT AAGAGAGTGT CAGAAGAATA TGGCTGCTGT 
TTAGGACAAG AGGTTGGATA CACTATTCGA TTTGAAGATT GTACAAGTCC AGAGACAGTC 
ATAAAGTACA TGACTGATGG TATGTTACTG AGAGAGTGCC TTATAGACCC CGACCTGACA 
CAGTATGCCA TAATCATGTT GGACGAGGCT CATGAGAGGA CAATTCATAC AGATGTGTTG 
TTTGGTCTCC TTAAAAAGAC TGTACAAAAG CGGCCAGATA TGAAGCTCAT TGTGACCTCT 
GCAACACTGG ATGCAGTCAA ATTCTCCCAG TATTTTTATG AGGCACCAAT TTTCACCATT 
CCCGGTCGGA CATACCCTGT TGAGATTCTT TATACCAAAG AGCCAGAGAC AGACTACCTG 
GATGCCAGTC TCATTACGGT CATGCAGATA CATTTGACAG AACCACCGGG GGATATTTTA 
GTATTCCTTA CTGGACAAGA GGAAATTGAT ACTGCTTGTG AAATTCTTTA TGAAAGAATG 
AAGTCTCTTG GACCTGATGT GCCAGAGCTG ATTATCTTAC CTGTTTATTC AGCATTGCCC 
AGTGAAATGC AGACAAGGAT ATTTGACCCA GCACCTCCTG GAAGCAGAAA GGTTGTGATT 
GCTACTAACA TTGCAGAGAC CTCCCTAACT ATTGATGGAA TATATTATGT GGTGGATCCA 
GGATTTGTGA AGCAGAAAGT TTACAACTCC AAGACAGGAA TTGATCAACT CGTAGTAACA 
CCAATTTCCC AGGCACAAGC TAAACAGCGA GCTGGGAGAG CAGGAAGAAC AGGACCTGGC 
AAGTGTTACC GGCTTTATAC TGAACGAGCC TATCGGGATG AAATGCTGAC CACTAATGTA 
CCTGAGATCC AAAGAACCAA CTTAGCAAGT ACAGTTCTGT CACTCAAGGC TATGGGAATC 
AATGATTTAC TCTCATTTGA TTTTATGGAT GCACCCCCAA TGGAGACACT GATCACTGCT 
ATGGAGCAGC TGTACACACT TGGTGCACTG GATGACGAAG GACTTCTTAC ACGCCTTGGC 
CGTAGAATGG CAGAGTTTCC TCTGGAGCCA ATGCTGTGTA AGATGTTGAT CATGTCTGTC 
CATCTTGGCT GTAGTGAGGA GATGTTAACC ATCGTCTCAA TGCTTTCAGT TCAGAATGTG 
TTCTACAGAC CCAAGGACAA ACAAGCTCTC GCAGACCAGA AAAAGGCAAA GTTCCACCAG 
ACAGAGGGAG ATCACCTGAC ACTACTCGCC GTTTATAATT CCTGGAAAAA CAATAAATTT 
TCCAATCCCT GGTGCTATGA AAATTTCATT CAGGCTCGTT CCCTACGCCG GGCTCAGGAT 
ATCCGGAAAC AGATGTTGGG AATAATGGAC AGGCATAAAC TCGATGTGGT ATCTTGTGGA 
AAAGCCACTG TGAGGGTACA GAAGGCAATA TGCAGTGGGT TTTTTCGAAA CGCAGCAAAA 
AAGGACCCTC AAGAGGGATA TCGGACTCTC ATAGACCAAC AGGTTGTCTA CATTCATCCA 
TCCAGCGCCC TTTTCAACAG GCAGCCAGAA TGGGTTGTGT ATCATGAACT TGTCCTGACT 
ACCAAAGAAT ACATGCGAGA AGTGACCACC ATAGATCCCC GCTGGCTTGT AGAATTTGCC 
CCGGCCTTCT TTAAAGTCTC TGACCCAACT AAGCTTAGCA AGCAGAAGAA ACAACAGAGG 
CTGGAGCCTC TCTACAACAG ATACGAGGAG CCCAACGCTT GGAGAATTTC CAGGGCATTC 
AGAAGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dhx8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム

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