ORF » Species Summary » Schizosaccharomyces pombe » prp22 cDNA ORF clone

prp22 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following prp22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prp22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102550 NM_001018650.2
Latest version!		 Schizosaccharomyces pombe ATP-dependent RNA helicase Prp22 (prp22), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    prp22 ( NM_001018650.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593253.1 Schizosaccharomyces pombe ATP-dependent RNA helicase Prp22 (prp22), mRNA.
    Price & Availability↑
    CloneID OSc102550
    Clone ID Related Accession (Same CDS sequence) NM_001018650.2
    Accession Version NM_001018650.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ATP-dependent RNA helicase Prp22
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018650.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGATC TGAAAGAATT GGAGTATTTA TCCTTGGTAT CTAAAGTTGC CTCTGAAATT 
    CGAAATCATA CTGGAATTGA CGATAATACT CTTGCAGAAT TTATTATTAA TTTGCATGAT 
    CAGTCTAAGA ATTACGATGA GTTTAAAAAT AATGTCCTTT CATGTGGAGG AGAATTCACC 
    GACAGTTTCC TTCAAAACAT AAGTCGATTA ATTAAGGAAA TTAAGCCTAA AGATGACATT 
    CCCACTGATA ATGTAAATAA TGGTAGTAAC TCGGTCAATG GAGCTTCGCA TGATTTAGAT 
    AGCAAAGATG TTGACAAACA ACACCAAAGA AAGATGTTTC CTGGCTTAAG CATCCCAAAT 
    AGCACGAATA ATTTAAGGGA TAGACCAGCG CTTATGGATA ATGCTATGGA CGAGCTGGAA 
    GAACTTTCGA CGTTGGCCAA AACAAGGAGA AACGATAGGG ATTCTAGGAG GGATGAGCGG 
    CATTACCTCA ATGGAATAAG GGAACGTAGA GAGAGATCAA TTAGTCCCAG TTTTTCGCAT 
    CATTCACGTA CCTCAATTTC TGGTCAATCA CATTCTAGTC GATCATCGCG TGGTCCATTG 
    CTAAATGCTC CAACATTGTA TGGAATTTAT TCTGGTGTTG TCTCAGGTAT TAAGGACTTT 
    GGTGCCTTTG TAACTTTGGA CGGCTTTAGA AAGAGAACTG ATGGTTTGGT TCATATCTCA 
    AATATCCAAT TAAATGGGAG ATTGGATCAT CCTTCAGAGG CTGTTAGCTA TGGACAACCT 
    GTATTTGTAA AAGTTATTAG AATTGATGAA TCAGCAAAGC GAATCAGCCT GTCAATGAAG 
    GAGGTGAATC AAGTTACAGG TGAGGATTTG AATCCGGATC AAGTTTCACG TTCTACAAAG 
    AAGGGTAGTG GAGCAAACGC TATTCCATTA TCAGCCCAAA ATTCTGAAAT TGGACATGTG 
    AATCCACTTG AAACGTTCAC TTCAAACGGT CGGAAGCGTT TAACATCACC TGAAATATGG 
    GAGTTACAGC AACTTGCTGC ATCCGGTGCA ATTTCCGCTA CGGATATTCC TGAACTCAAC 
    GATGGATTCA ATACTAATAA TGCTGCGGAA ATTAATCCCG AAGATGATGA AGACGTAGAA 
    ATCGAACTTC GTGAAGAAGA ACCTGGATTT CTCGCAGGAC AAACCAAAGT TTCATTAAAG 
    CTATCTCCTA TAAAAGTTGT AAAGGCCCCG GATGGTTCAT TGTCCAGAGC AGCAATGCAA 
    GGGCAGATTT TAGCAAATGA TAGACGAGAA ATAAGACAAA AGGAGGCAAA ATTAAAGAGT 
    GAACAAGAAA TGGAAAAGCA AGACTTATCC CTCTCATGGC AAGACACTAT GTCGAATCCT 
    CAGGATCGGA AATTTGCCCA AGATGTGCGA GACTCAGCTG CTCGTCAACT TACTTCTGAA 
    ACTCCCAGCT GGCGTCAAGC AACACGAAAT GCAAATATAT CCTACGGTAA AAGAACAACA 
    CTGAGCATGA AAGAGCAAAG AGAGGGTCTT CCAGTTTTTA AACTTCGTAA ACAGTTTCTC 
    GAGGCTGTCT CAAAAAACCA AATTTTGGTT CTACTCGGTG AAACCGGTTC CGGTAAGACC 
    ACTCAAATCA CTCAATATCT TGCTGAAGAA GGTTATACCA GTGATAGCAA AATGATAGGT 
    TGTACTCAAC CTAGGCGTGT AGCTGCAATG TCTGTTGCCA AACGTGTAGC AGAAGAAGTG 
    GGATGTCGTG TTGGTGAAGA GGTTGGTTAC ACTATTCGTT TCGAAGATAA GACCAGTAGA 
    ATGACCCAAA TTAAATATAT GACTGATGGT ATGCTTCAAC GAGAATGTTT AGTTGATCCT 
    CTTTTGTCCA AATATTCTGT AATTATTTTA GATGAAGCAC ATGAAAGAAC CGTTGCAACT 
    GATGTACTTT TTGGACTGTT AAAGGGAACT GTTTTGAAAC GGCCAGATTT AAAGCTAATT 
    GTTACTTCAG CCACGCTAGA TGCTGAGCGG TTTTCGTCTT ATTTCTATAA ATGCCCAATT 
    TTTACGATTC CTGGGCGTTC ATATCCCGTC GAAATCATGT ATACAAAACA ACCAGAAGCC 
    GACTATCTTG ATGCTGCTTT GATGACTGTT ATGCAAATCC ACTTGAGCGA AGGACCTGGT 
    GATATATTAG TATTTCTGAC AGGTCAAGAA GAAATTGATA CTAGTTGTGA AATATTATAT 
    GAAAGAAGTA AAATGCTAGG AGATTCGATT CCTGAGCTAG TTATTTTACC TGTTTATTCA 
    GCGCTTCCTT CGGAAATACA AAGTCGTATT TTTGAACCTG CTCCCCCAGG AGGTCGTAAG 
    GTAGTAATTG CAACCAACAT TGCTGAAACT AGTTTAACAA TTGATGGAAT TTATTATGTG 
    GTGGACCCTG GGTTTGTCAA GCAGAGCTGC TTTGATCCAA AACTAGGCAT GGATTCTCTA 
    ATAGTTACGC CAATTTCTCA AGCACAAGCT AGGCAAAGAA GTGGTCGTGC AGGGCGTACA 
    GGACCCGGAA AATGTTATCG TCTGTATACT GAAAGCGCTT ACCGAAACGA AATGTTGCCT 
    TCCCCGATTC CTGAGATACA ACGCCAAAAT TTGTCGCATA CTATTTTGAT GTTAAAGGCA 
    ATGGGAATTA ACGACCTTTT GAATTTTGAT TTTATGGATC CTCCACCTGC TCAAACCATG 
    ATAGCCGCCT TGCAAAATCT GTATGCACTG TCGGCCTTGG ACGATGAGGG ATTGCTGACT 
    CCTCTTGGGC GAAAAATGGC AGATTTCCCG ATGGAGCCTC AATTGTCAAA AGTATTGATT 
    ACTAGCGTAG AATTAGGTTG CTCTGAAGAA ATGTTGAGTA TTATTGCCAT GCTTAGCGTA 
    CCTAACATTT GGAGCCGACC AAGGGAGAAG CAGCAAGAAG CTGATCGACA ACGAGCACAA 
    TTTGCAAATC CTGAGAGTGA TCACTTAACC TTACTTAATG TGTATACGAC TTGGAAAATG 
    AATCGTTGCT CAGATAATTG GTGTTATGAA CATTATATCC AAGCCCGTGG TATGCGTCGA 
    GCTGAAGATG TACGCAAACA GCTTATTCGC CTAATGGATC GGTATCGACA CCCAGTTGTT 
    TCATGTGGAA GAAAGAGAGA ACTTATTTTA CGGGCTTTGT GTTCTGGCTA CTTTACCAAT 
    GTTGCCAAGC GAGACTCTCA CGAGGGATGT TACAAAACGA TCGTGGAGAA TGCTCCTGTA 
    TACATGCATC CTAGTGGAGT TTTATTTGGA AAAGCTGCAG AGTGGGTGAT ATACCATGAA 
    CTGATTCAAA CGTCAAAAGA ATACATGCAC ACGGTAAGCA CAGTGAATCC AAAATGGCTT 
    GTGGAAGTTG CACCAACATT CTTTAAATTT GCCAATGCAA ATCAAGTTTC CAAAACCAAG 
    AAAAATCTCA AGGTGCTACC ATTGTACAAT CGATTTGAAA AACCCGATGA GTGGAGGATT 
    TCAAAGCAAC GAAAGGGAGG TCGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593253.1
    CDS147..3653
    Translation

    Target ORF information:

    RefSeq Version NM_001018650.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ATP-dependent RNA helicase Prp22 (prp22), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018650.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGACGATC TGAAAGAATT GGAGTATTTA TCCTTGGTAT CTAAAGTTGC CTCTGAAATT 
    CGAAATCATA CTGGAATTGA CGATAATACT CTTGCAGAAT TTATTATTAA TTTGCATGAT 
    CAGTCTAAGA ATTACGATGA GTTTAAAAAT AATGTCCTTT CATGTGGAGG AGAATTCACC 
    GACAGTTTCC TTCAAAACAT AAGTCGATTA ATTAAGGAAA TTAAGCCTAA AGATGACATT 
    CCCACTGATA ATGTAAATAA TGGTAGTAAC TCGGTCAATG GAGCTTCGCA TGATTTAGAT 
    AGCAAAGATG TTGACAAACA ACACCAAAGA AAGATGTTTC CTGGCTTAAG CATCCCAAAT 
    AGCACGAATA ATTTAAGGGA TAGACCAGCG CTTATGGATA ATGCTATGGA CGAGCTGGAA 
    GAACTTTCGA CGTTGGCCAA AACAAGGAGA AACGATAGGG ATTCTAGGAG GGATGAGCGG 
    CATTACCTCA ATGGAATAAG GGAACGTAGA GAGAGATCAA TTAGTCCCAG TTTTTCGCAT 
    CATTCACGTA CCTCAATTTC TGGTCAATCA CATTCTAGTC GATCATCGCG TGGTCCATTG 
    CTAAATGCTC CAACATTGTA TGGAATTTAT TCTGGTGTTG TCTCAGGTAT TAAGGACTTT 
    GGTGCCTTTG TAACTTTGGA CGGCTTTAGA AAGAGAACTG ATGGTTTGGT TCATATCTCA 
    AATATCCAAT TAAATGGGAG ATTGGATCAT CCTTCAGAGG CTGTTAGCTA TGGACAACCT 
    GTATTTGTAA AAGTTATTAG AATTGATGAA TCAGCAAAGC GAATCAGCCT GTCAATGAAG 
    GAGGTGAATC AAGTTACAGG TGAGGATTTG AATCCGGATC AAGTTTCACG TTCTACAAAG 
    AAGGGTAGTG GAGCAAACGC TATTCCATTA TCAGCCCAAA ATTCTGAAAT TGGACATGTG 
    AATCCACTTG AAACGTTCAC TTCAAACGGT CGGAAGCGTT TAACATCACC TGAAATATGG 
    GAGTTACAGC AACTTGCTGC ATCCGGTGCA ATTTCCGCTA CGGATATTCC TGAACTCAAC 
    GATGGATTCA ATACTAATAA TGCTGCGGAA ATTAATCCCG AAGATGATGA AGACGTAGAA 
    ATCGAACTTC GTGAAGAAGA ACCTGGATTT CTCGCAGGAC AAACCAAAGT TTCATTAAAG 
    CTATCTCCTA TAAAAGTTGT AAAGGCCCCG GATGGTTCAT TGTCCAGAGC AGCAATGCAA 
    GGGCAGATTT TAGCAAATGA TAGACGAGAA ATAAGACAAA AGGAGGCAAA ATTAAAGAGT 
    GAACAAGAAA TGGAAAAGCA AGACTTATCC CTCTCATGGC AAGACACTAT GTCGAATCCT 
    CAGGATCGGA AATTTGCCCA AGATGTGCGA GACTCAGCTG CTCGTCAACT TACTTCTGAA 
    ACTCCCAGCT GGCGTCAAGC AACACGAAAT GCAAATATAT CCTACGGTAA AAGAACAACA 
    CTGAGCATGA AAGAGCAAAG AGAGGGTCTT CCAGTTTTTA AACTTCGTAA ACAGTTTCTC 
    GAGGCTGTCT CAAAAAACCA AATTTTGGTT CTACTCGGTG AAACCGGTTC CGGTAAGACC 
    ACTCAAATCA CTCAATATCT TGCTGAAGAA GGTTATACCA GTGATAGCAA AATGATAGGT 
    TGTACTCAAC CTAGGCGTGT AGCTGCAATG TCTGTTGCCA AACGTGTAGC AGAAGAAGTG 
    GGATGTCGTG TTGGTGAAGA GGTTGGTTAC ACTATTCGTT TCGAAGATAA GACCAGTAGA 
    ATGACCCAAA TTAAATATAT GACTGATGGT ATGCTTCAAC GAGAATGTTT AGTTGATCCT 
    CTTTTGTCCA AATATTCTGT AATTATTTTA GATGAAGCAC ATGAAAGAAC CGTTGCAACT 
    GATGTACTTT TTGGACTGTT AAAGGGAACT GTTTTGAAAC GGCCAGATTT AAAGCTAATT 
    GTTACTTCAG CCACGCTAGA TGCTGAGCGG TTTTCGTCTT ATTTCTATAA ATGCCCAATT 
    TTTACGATTC CTGGGCGTTC ATATCCCGTC GAAATCATGT ATACAAAACA ACCAGAAGCC 
    GACTATCTTG ATGCTGCTTT GATGACTGTT ATGCAAATCC ACTTGAGCGA AGGACCTGGT 
    GATATATTAG TATTTCTGAC AGGTCAAGAA GAAATTGATA CTAGTTGTGA AATATTATAT 
    GAAAGAAGTA AAATGCTAGG AGATTCGATT CCTGAGCTAG TTATTTTACC TGTTTATTCA 
    GCGCTTCCTT CGGAAATACA AAGTCGTATT TTTGAACCTG CTCCCCCAGG AGGTCGTAAG 
    GTAGTAATTG CAACCAACAT TGCTGAAACT AGTTTAACAA TTGATGGAAT TTATTATGTG 
    GTGGACCCTG GGTTTGTCAA GCAGAGCTGC TTTGATCCAA AACTAGGCAT GGATTCTCTA 
    ATAGTTACGC CAATTTCTCA AGCACAAGCT AGGCAAAGAA GTGGTCGTGC AGGGCGTACA 
    GGACCCGGAA AATGTTATCG TCTGTATACT GAAAGCGCTT ACCGAAACGA AATGTTGCCT 
    TCCCCGATTC CTGAGATACA ACGCCAAAAT TTGTCGCATA CTATTTTGAT GTTAAAGGCA 
    ATGGGAATTA ACGACCTTTT GAATTTTGAT TTTATGGATC CTCCACCTGC TCAAACCATG 
    ATAGCCGCCT TGCAAAATCT GTATGCACTG TCGGCCTTGG ACGATGAGGG ATTGCTGACT 
    CCTCTTGGGC GAAAAATGGC AGATTTCCCG ATGGAGCCTC AATTGTCAAA AGTATTGATT 
    ACTAGCGTAG AATTAGGTTG CTCTGAAGAA ATGTTGAGTA TTATTGCCAT GCTTAGCGTA 
    CCTAACATTT GGAGCCGACC AAGGGAGAAG CAGCAAGAAG CTGATCGACA ACGAGCACAA 
    TTTGCAAATC CTGAGAGTGA TCACTTAACC TTACTTAATG TGTATACGAC TTGGAAAATG 
    AATCGTTGCT CAGATAATTG GTGTTATGAA CATTATATCC AAGCCCGTGG TATGCGTCGA 
    GCTGAAGATG TACGCAAACA GCTTATTCGC CTAATGGATC GGTATCGACA CCCAGTTGTT 
    TCATGTGGAA GAAAGAGAGA ACTTATTTTA CGGGCTTTGT GTTCTGGCTA CTTTACCAAT 
    GTTGCCAAGC GAGACTCTCA CGAGGGATGT TACAAAACGA TCGTGGAGAA TGCTCCTGTA 
    TACATGCATC CTAGTGGAGT TTTATTTGGA AAAGCTGCAG AGTGGGTGAT ATACCATGAA 
    CTGATTCAAA CGTCAAAAGA ATACATGCAC ACGGTAAGCA CAGTGAATCC AAAATGGCTT 
    GTGGAAGTTG CACCAACATT CTTTAAATTT GCCAATGCAA ATCAAGTTTC CAAAACCAAG 
    AAAAATCTCA AGGTGCTACC ATTGTACAAT CGATTTGAAA AACCCGATGA GTGGAGGATT 
    TCAAAGCAAC GAAAGGGAGG TCGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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