ORF » Species Summary » Saccharomyces cerevisiae S288C » PRP22 cDNA ORF clone

PRP22 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following PRP22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OSi05500 NM_001178904.3
Latest version!		 Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP22 (PRP22), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PRP22 ( NM_001178904.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010929.3 Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP22 (PRP22), partial mRNA.
    Price & Availability↑
    CloneID OSi05500
    Clone ID Related Accession (Same CDS sequence) NM_001178904.3
    Accession Version NM_001178904.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product DEAH-box ATP-dependent RNA helicase PRP22
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001178904.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTGATA TATCGAAACT CATAGGAGCT ATTGTGGGAT CTGATGACCC CGTTATCATT 
    GAATTTGTGC TGAATATCAT AAATAAATCC GGCAATTTAC AAGAGTTTAT ACGAAATATT 
    CAAAAATTGG ATGCCGGTAT ATCCTACGAA GACAGTATTA AGATGTATAA TGCATTTTTA 
    GGAAAGCAGG AAGAAGAAAA GGTACGAAAT AAAGTTAAAA GTTCACCGTT AAGTCAAAAA 
    ATTAATCAGG TACTGAAAGA TGACGTTAAT TTGGACGACC CTGTTGTGAC TGAATTCGTC 
    CTGAGCATTT TGAATAAATC TAAATCAATA ACTGAGTTCC AGGAACAACT GAACTTGATG 
    CAAAGCGGAC TCGATAATGA AACAATTTTC AAGATATATC AAATTGCATC ACCACCAGTA 
    ATGAAAGAAG AAGTCTCAGT TCTTCCAAGC ACCAAGATAC CAGCAAAGAT AGAAGCAAAG 
    ATTGAAGAAG AGGTACAAAA AATAGAAAGT TTAGACCCCA GCCCTGTCCT TCACAAAGTT 
    TATGAAGGTA AAGTGAGAAA CATAACTACT TTTGGGTGTT TCGTCCAAAT TTTTGGGACG 
    CGAATGAAGA ATTGTGATGG CTTAGTTCAT ATTTCAGAAA TGTCAGACCA AAGAACATTA 
    GATCCACATG ATGTGGTGAG ACAAGGCCAA CATATCTTTG TCGAAGTGAT AAAAATACAA 
    AATAATGGAA AAATATCGCT CTCCATGAAA AATATTGATC AACATAGTGG AGAAATCCGG 
    AAAAGAAATA CTGAAAGTGT CGAAGATAGA GGTAGGTCCA ACGATGCTCA TACAAGCAGA 
    AATATGAAAA ACAAAATTAA AAGGCGTGCT TTGACTTCAC CAGAAAGATG GGAAATTCGC 
    CAACTAATTG CCAGCGGTGC TGCTTCCATC GATGACTATC CTGAATTGAA GGATGAAATA 
    CCCATAAATA CTTCCTATTT GACCGCGAAA AGAGATGACG GCAGTATCGT TAACGGTAAT 
    ACAGAGAAAG TGGACTCAAA ATTGGAAGAA CAACAACGAG ACGAGACGGA TGAAATTGAT 
    GTCGAGCTGA ATACAGATGA TGGGCCCAAA TTTTTAAAGG ATCAGCAAGT CAAGGGGGCC 
    AAGAAATATG AAATGCCTAA AATTACAAAA GTCCCGAGAG GATTTATGAA TCGTTCAGCA 
    ATCAACGGCT CTAATGCAAT ACGAGATCAT AGAGAAGAAA AGTTAAGGAA AAAAAGAGAG 
    ATTGAACAAC AGATTAGGAA ACAACAGTCG TTTGATGATC CTACGAAAAA CAAAAAAGAT 
    TCCAGGAACG AAATTCAAAT GTTGAAAAAC CAGCTGATTG TTACTGAATG GGAAAAAAAT 
    AGAATGAATG AATCTATCTC GTATGGCAAA AGAACTTCCT TACCAATCAG TGCACAGCGT 
    CAAACTTTGC CTGTATACGC GATGAGGTCT GAATTGATAC AGGCTGTGCG TGACAATCAA 
    TTTCTGGTCA TTGTTGGTGA GACAGGTTCG GGTAAAACCA CTCAAATTAC TCAGTATTTA 
    GATGAAGAAG GGTTTAGTAA CTATGGGATG ATCGGATGTA CCCAACCTCG TAGGGTTGCC 
    GCTGTATCCG TTGCTAAAAG AGTGGCTGAA GAAGTTGGCT GCAAAGTTGG CCATGACGTT 
    GGCTATACCA TTAGATTTGA AGATGTTACT GGCCCAGATA CCAGAATAAA ATATATGACT 
    GATGGTATGT TACAGAGAGA GGCGTTGTTA GATCCCGAGA TGTCAAAATA TTCAGTTATC 
    ATGTTAGATG AAGCTCACGA AAGAACTGTG GCAACTGATG TTTTATTTGC GTTGTTAAAG 
    AAAGCGGCTA TTAAAAGGCC AGAATTAAAA GTAATAGTTA CATCGGCTAC TTTGAACTCT 
    GCTAAATTTT CTGAGTACTT TTTAAATTGT CCGATTATCA ATATACCTGG TAAAACTTTT 
    CCCGTGGAAG TACTCTACTC ACAGACGCCC CAAATGGATT ACATAGAGGC AGCTTTGGAT 
    TGTGTAATAG ACATCCATAT TAATGAAGGC CCAGGCGATA TTTTAGTCTT TTTGACTGGA 
    CAGGAAGAAA TAGACTCATG CTGTGAAATT TTATATGATA GGGTGAAAAC TTTAGGTGAC 
    AGCATCGGCG AATTACTGAT TTTGCCCGTT TATTCTGCTT TGCCAAGTGA AATCCAATCG 
    AAAATTTTTG AACCCACTCC AAAGGGCAGT CGAAAAGTCG TATTTGCCAC AAATATTGCG 
    GAAACTTCAA TCACTATTGA TGGAATTTAT TATGTCGTGG ACCCTGGATT TGCAAAAATC 
    AACATTTATA ATGCAAGAGC AGGTATCGAG CAGTTGATAG TGTCTCCAAT CTCTCAGGCA 
    CAGGCAAATC AAAGAAAAGG TAGAGCTGGA AGAACAGGCC CTGGAAAATG CTATCGACTT 
    TATACAGAAT CCGCATTTTA CAATGAAATG TTAGAAAACA CGGTTCCAGA AATTCAAAGG 
    CAGAATCTTT CTCATACTAT TTTGATGCTA AAGGCCATGG GAATTAATGA TTTATTGAAA 
    TTTGACTTCA TGGATCCCCC TCCCAAAAAT TTAATGCTTA ACGCACTGAC AGAATTATAC 
    CACCTGCAGT CACTAGATGA CGAGGGAAAA CTAACAAATT TAGGTAAAGA GATGTCCTTA 
    TTTCCAATGG ATCCCACTCT ATCACGCTCT TTATTGTCAT CTGTTGATAA TCAGTGCTCG 
    GATGAAATTG TTACTATAAT CTCAATGTTG TCAGTACAGA ACGTTTTTTA TCGTCCAAAA 
    GATAGGCAAT TGGAAGCAGA TAGCAAGAAG GCAAAGTTTC ATCATCCATA CGGTGACCAT 
    TTAACATTAT TGAACGTTTA TACTAGATGG CAGCAGGCAA ATTACTCGGA GCAGTATTGC 
    AAAACAAACT TTCTGCATTT TAGACATTTG AAAAGAGCAA GAGATGTTAA AAGCCAAATT 
    AGCATGATAT TTAAAAAGAT AGGCTTAAAG TTAATAAGCT GTCATAGTGA TCCTGATTTA 
    ATTCGTAAAA CTTTCGTGTC AGGGTTCTTC ATGAACGCTG CTAAGCGAGA CTCACAGGTA 
    GGTTATAAAA CCATCAATGG TGGTACAGAG GTTGGAATAC ATCCCTCGAG TTCATTGTAC 
    GGAAAAGAGT ATGAATACGT AATGTATCAT AGTATCGTAT TAACTAGCCG GGAATACATG 
    TCGCAGGTAA CATCCATTGA GCCCCAATGG CTTCTCGAAG TAGCGCCTCA TTTTTATAAA 
    GCTGGAGATG CTGAAAGCCA GTCTAGGAAG AAAGCGAAAA TCATTCCTTT GCATAACAAG 
    TTTGCCAAGG ATCAAAATTC ATGGAGACTA AGCTCAATAA GGCAGTCAAG GGAAAGGGCA 
    TTAGGTATCA AGAGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010929.3
    CDS1..3438
    Translation

    Target ORF information:

    RefSeq Version NM_001178904.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP22 (PRP22), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178904.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTGATA TATCGAAACT CATAGGAGCT ATTGTGGGAT CTGATGACCC CGTTATCATT 
    GAATTTGTGC TGAATATCAT AAATAAATCC GGCAATTTAC AAGAGTTTAT ACGAAATATT 
    CAAAAATTGG ATGCCGGTAT ATCCTACGAA GACAGTATTA AGATGTATAA TGCATTTTTA 
    GGAAAGCAGG AAGAAGAAAA GGTACGAAAT AAAGTTAAAA GTTCACCGTT AAGTCAAAAA 
    ATTAATCAGG TACTGAAAGA TGACGTTAAT TTGGACGACC CTGTTGTGAC TGAATTCGTC 
    CTGAGCATTT TGAATAAATC TAAATCAATA ACTGAGTTCC AGGAACAACT GAACTTGATG 
    CAAAGCGGAC TCGATAATGA AACAATTTTC AAGATATATC AAATTGCATC ACCACCAGTA 
    ATGAAAGAAG AAGTCTCAGT TCTTCCAAGC ACCAAGATAC CAGCAAAGAT AGAAGCAAAG 
    ATTGAAGAAG AGGTACAAAA AATAGAAAGT TTAGACCCCA GCCCTGTCCT TCACAAAGTT 
    TATGAAGGTA AAGTGAGAAA CATAACTACT TTTGGGTGTT TCGTCCAAAT TTTTGGGACG 
    CGAATGAAGA ATTGTGATGG CTTAGTTCAT ATTTCAGAAA TGTCAGACCA AAGAACATTA 
    GATCCACATG ATGTGGTGAG ACAAGGCCAA CATATCTTTG TCGAAGTGAT AAAAATACAA 
    AATAATGGAA AAATATCGCT CTCCATGAAA AATATTGATC AACATAGTGG AGAAATCCGG 
    AAAAGAAATA CTGAAAGTGT CGAAGATAGA GGTAGGTCCA ACGATGCTCA TACAAGCAGA 
    AATATGAAAA ACAAAATTAA AAGGCGTGCT TTGACTTCAC CAGAAAGATG GGAAATTCGC 
    CAACTAATTG CCAGCGGTGC TGCTTCCATC GATGACTATC CTGAATTGAA GGATGAAATA 
    CCCATAAATA CTTCCTATTT GACCGCGAAA AGAGATGACG GCAGTATCGT TAACGGTAAT 
    ACAGAGAAAG TGGACTCAAA ATTGGAAGAA CAACAACGAG ACGAGACGGA TGAAATTGAT 
    GTCGAGCTGA ATACAGATGA TGGGCCCAAA TTTTTAAAGG ATCAGCAAGT CAAGGGGGCC 
    AAGAAATATG AAATGCCTAA AATTACAAAA GTCCCGAGAG GATTTATGAA TCGTTCAGCA 
    ATCAACGGCT CTAATGCAAT ACGAGATCAT AGAGAAGAAA AGTTAAGGAA AAAAAGAGAG 
    ATTGAACAAC AGATTAGGAA ACAACAGTCG TTTGATGATC CTACGAAAAA CAAAAAAGAT 
    TCCAGGAACG AAATTCAAAT GTTGAAAAAC CAGCTGATTG TTACTGAATG GGAAAAAAAT 
    AGAATGAATG AATCTATCTC GTATGGCAAA AGAACTTCCT TACCAATCAG TGCACAGCGT 
    CAAACTTTGC CTGTATACGC GATGAGGTCT GAATTGATAC AGGCTGTGCG TGACAATCAA 
    TTTCTGGTCA TTGTTGGTGA GACAGGTTCG GGTAAAACCA CTCAAATTAC TCAGTATTTA 
    GATGAAGAAG GGTTTAGTAA CTATGGGATG ATCGGATGTA CCCAACCTCG TAGGGTTGCC 
    GCTGTATCCG TTGCTAAAAG AGTGGCTGAA GAAGTTGGCT GCAAAGTTGG CCATGACGTT 
    GGCTATACCA TTAGATTTGA AGATGTTACT GGCCCAGATA CCAGAATAAA ATATATGACT 
    GATGGTATGT TACAGAGAGA GGCGTTGTTA GATCCCGAGA TGTCAAAATA TTCAGTTATC 
    ATGTTAGATG AAGCTCACGA AAGAACTGTG GCAACTGATG TTTTATTTGC GTTGTTAAAG 
    AAAGCGGCTA TTAAAAGGCC AGAATTAAAA GTAATAGTTA CATCGGCTAC TTTGAACTCT 
    GCTAAATTTT CTGAGTACTT TTTAAATTGT CCGATTATCA ATATACCTGG TAAAACTTTT 
    CCCGTGGAAG TACTCTACTC ACAGACGCCC CAAATGGATT ACATAGAGGC AGCTTTGGAT 
    TGTGTAATAG ACATCCATAT TAATGAAGGC CCAGGCGATA TTTTAGTCTT TTTGACTGGA 
    CAGGAAGAAA TAGACTCATG CTGTGAAATT TTATATGATA GGGTGAAAAC TTTAGGTGAC 
    AGCATCGGCG AATTACTGAT TTTGCCCGTT TATTCTGCTT TGCCAAGTGA AATCCAATCG 
    AAAATTTTTG AACCCACTCC AAAGGGCAGT CGAAAAGTCG TATTTGCCAC AAATATTGCG 
    GAAACTTCAA TCACTATTGA TGGAATTTAT TATGTCGTGG ACCCTGGATT TGCAAAAATC 
    AACATTTATA ATGCAAGAGC AGGTATCGAG CAGTTGATAG TGTCTCCAAT CTCTCAGGCA 
    CAGGCAAATC AAAGAAAAGG TAGAGCTGGA AGAACAGGCC CTGGAAAATG CTATCGACTT 
    TATACAGAAT CCGCATTTTA CAATGAAATG TTAGAAAACA CGGTTCCAGA AATTCAAAGG 
    CAGAATCTTT CTCATACTAT TTTGATGCTA AAGGCCATGG GAATTAATGA TTTATTGAAA 
    TTTGACTTCA TGGATCCCCC TCCCAAAAAT TTAATGCTTA ACGCACTGAC AGAATTATAC 
    CACCTGCAGT CACTAGATGA CGAGGGAAAA CTAACAAATT TAGGTAAAGA GATGTCCTTA 
    TTTCCAATGG ATCCCACTCT ATCACGCTCT TTATTGTCAT CTGTTGATAA TCAGTGCTCG 
    GATGAAATTG TTACTATAAT CTCAATGTTG TCAGTACAGA ACGTTTTTTA TCGTCCAAAA 
    GATAGGCAAT TGGAAGCAGA TAGCAAGAAG GCAAAGTTTC ATCATCCATA CGGTGACCAT 
    TTAACATTAT TGAACGTTTA TACTAGATGG CAGCAGGCAA ATTACTCGGA GCAGTATTGC 
    AAAACAAACT TTCTGCATTT TAGACATTTG AAAAGAGCAA GAGATGTTAA AAGCCAAATT 
    AGCATGATAT TTAAAAAGAT AGGCTTAAAG TTAATAAGCT GTCATAGTGA TCCTGATTTA 
    ATTCGTAAAA CTTTCGTGTC AGGGTTCTTC ATGAACGCTG CTAAGCGAGA CTCACAGGTA 
    GGTTATAAAA CCATCAATGG TGGTACAGAG GTTGGAATAC ATCCCTCGAG TTCATTGTAC 
    GGAAAAGAGT ATGAATACGT AATGTATCAT AGTATCGTAT TAACTAGCCG GGAATACATG 
    TCGCAGGTAA CATCCATTGA GCCCCAATGG CTTCTCGAAG TAGCGCCTCA TTTTTATAAA 
    GCTGGAGATG CTGAAAGCCA GTCTAGGAAG AAAGCGAAAA TCATTCCTTT GCATAACAAG 
    TTTGCCAAGG ATCAAAATTC ATGGAGACTA AGCTCAATAA GGCAGTCAAG GGAAAGGGCA 
    TTAGGTATCA AGAGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
    Nature387(6632 Suppl)78-81(1997 May)
    Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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