DHX8 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001302623.1	NP_001289552.1	ATP-dependent RNA helicase DHX8 isoform 2
NM_001322218.2	NP_001309147.1	ATP-dependent RNA helicase DHX8 isoform 5
NM_001322221.1	NP_001309150.1	ATP-dependent RNA helicase DHX8 isoform 8
NM_004941.3	NP_004932.1	ATP-dependent RNA helicase DHX8 isoform 1
NM_001322219.1	NP_001309148.1	ATP-dependent RNA helicase DHX8 isoform 6
NM_001322219.2	NP_001309148.1	ATP-dependent RNA helicase DHX8 isoform 6
NM_001322220.1	NP_001309149.1	ATP-dependent RNA helicase DHX8 isoform 7
NM_004941.2	NP_004932.1	ATP-dependent RNA helicase DHX8 isoform 1
NM_001322218.1	NP_001309147.1	ATP-dependent RNA helicase DHX8 isoform 5
NM_001322220.2	NP_001309149.1	ATP-dependent RNA helicase DHX8 isoform 7
NM_001302623.2	NP_001289552.1	ATP-dependent RNA helicase DHX8 isoform 2
NM_001322216.1	NP_001309145.1	ATP-dependent RNA helicase DHX8 isoform 3
NM_001322217.1	NP_001309146.1	ATP-dependent RNA helicase DHX8 isoform 4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13153	NM_004941.3 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu101910	NM_001322216.1 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu101677	NM_001322220.2 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu101742	NM_001322218.2 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu101754	NM_001322219.2 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu101781	NM_001322217.1 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu50170	NM_001322221.1 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu50171	NM_001302623.2 Latest version!	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu101754	NM_001322219.1	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu13153	NM_004941.2	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu101742	NM_001322218.1	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu101677	NM_001322220.1	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu50171	NM_001302623.1	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

DHX8 ( NM_004941.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004932.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13153
Clone ID Related Accession (Same CDS sequence)	NM_004941.3 , NM_004941.2
Accession Version	NM_004941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D50487.1, BC044586.1 and AC068675.17. On May 17, 2019 this sequence version replaced NM_004941.2. Transcript Variant:. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.27203.1, SRR1660807.238146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262415.8/ ENSP00000262415.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGTGGTG TACCATGAGC TGGTGCTCAC CACCAAGGAA 
TACATGCGTG AAGTTACCAC CATCGACCCT CGGTGGCTTG TGGAGTTTGC CCCAGCCTTC 
TTCAAGGTCT CAGACCCAAC TAAGCTAAGC AAACAGAAGA AGCAACAGCG TCTTGAACCC 
TTGTACAACC GCTATGAGGA ACCCAATGCC TGGAGAATAT CTCGAGCTTT CCGACGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004932.1
CDS	64..3726
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	NM_004941.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004941.3

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGTGGTG TACCATGAGC TGGTGCTCAC CACCAAGGAA 
TACATGCGTG AAGTTACCAC CATCGACCCT CGGTGGCTTG TGGAGTTTGC CCCAGCCTTC 
TTCAAGGTCT CAGACCCAAC TAAGCTAAGC AAACAGAAGA AGCAACAGCG TCTTGAACCC 
TTGTACAACC GCTATGAGGA ACCCAATGCC TGGAGAATAT CTCGAGCTTT CCGACGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322216.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309145.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu101910
Clone ID Related Accession (Same CDS sequence)	NM_001322216.1
Accession Version	NM_001322216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.176012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTTTG GTTGCTTTGT GCAGCTGGAA GGACTAAGGA AGCGGTGGGA AGGCCTGGTG 
CACATCTCTG AGCTCCGGCG GGAGGGTCGT GTGGCCAATG TAGCTGATGT CGTGAGCAAA 
GGCCAGAGGG TCAAAGTCAA AGTGCTGTCC TTCACTGGGA CCAAGACCAG CCTGAGCATG 
AAGGATGTGG ATCAAGAGAC TGGAGAAGAT CTAAACCCAA ATAGACGGCG AAATCTTGTC 
GGGGAGACCA ATGAGGAGAC CTCAATGCGG AATCCTGATA GACCCACTCA CTTGTCCCTT 
GTCAGTGCTC CTGAAGTAGA GGACGACTCA CTGGAACGCA AGCGCCTCAC CCGAATCTCT 
GACCCAGAGA AGTGGGAGAT CAAACAGATG ATTGCTGCCA ATGTCCTTTC CAAAGAAGAA 
TTTCCAGACT TTGATGAAGA GACTGGCATT CTCCCTAAGG TGGATGATGA AGAAGATGAG 
GACCTTGAGA TTGAATTGGT TGAGGAAGAG CCTCCATTCC TGAGAGGGCA CACTAAGCAA 
AGCATGGACA TGAGCCCCAT TAAAATTGTC AAGAACCCAG ACGGCTCCCT CTCCCAAGCA 
GCAATGATGC AGAGTGCCTT GGCCAAAGAA AGGCGGGAAC TCAAACAGGC CCAGCGGGAA 
GCTGAGATGG ATTCTATTCC CATGGGACTC AACAAACACT GGGTTGACCC TCTGCCTGAT 
GCGGAAGGCA GACAGATTGC TGCCAACATG AGGGGTATTG GGATGATGCC CAATGATATT 
CCTGAGTGGA AGAAGCATGC CTTTGGGGGC AACAAAGCCT CTTACGGAAA AAAGACCCAG 
ATGTCAATCC TTGAGCAGAG GGAGAGCCTG CCCATCTACA AACTGAAGGA GCAATTGGTC 
CAGGCCGTCC ATGACAATCA GATCCTGATT GTCATTGGTG AGACAGGATC TGGAAAGACA 
ACACAGATCA CCCAGTACCT GGCGGAGGCA GGCTACACTT CCAGGGGCAA GATTGGGTGT 
ACCCAGCCCA GAAGAGTGGC AGCTATGTCG GTGGCCAAAA GAGTGTCAGA GGAGTTTGGT 
TGTTGCTTAG GCCAAGAGGT GGGCTACACC ATTCGATTTG AGGACTGCAC TAGCCCTGAA 
ACAGTCATCA AGTACATGAC AGATGGGATG TTGCTTAGAG AGTGCTTGAT TGACCCTGAC 
CTCACTCAGT ACGCGATCAT CATGTTGGAC GAGGCACATG AGAGGACAAT TCACACTGAT 
GTGCTCTTTG GATTGTTGAA AAAGACAGTT CAGAAACGGC AGGACATGAA GCTGATTGTC 
ACCTCAGCCA CCTTGGATGC AGTGAAGTTT TCTCAATACT TCTATGAAGC TCCCATTTTC 
ACCATCCCAG GTCGAACATA TCCAGTGGAA ATACTGTACA CAAAGGAACC TGAGACAGAT 
TATCTGGATG CCAGCCTGAT TACTGTTATG CAGATTCATT TAACAGAACC ACCAGGTGAT 
ATCCTGGTCT TCCTGACTGG TCAGGAAGAA ATTGATACTG CTTGTGAGAT CCTGTATGAA 
AGAATGAAAT CCCTGGGACC TGATGTTCCA GAGTTAATTA TCCTCCCAGT GTACTCTGCT 
CTTCCCAGTG AGATGCAGAC CCGAATCTTT GACCCAGCTC CACCAGGCAG CAGAAAGGTT 
GTGATTGCCA CCAATATCGC AGAGACATCG CTGACTATTG ATGGTATCTA CTATGTGGTG 
GACCCAGGAT TCGTGAAACA GAAAGTTTAC AATTCCAAGA CAGGGATTGA CCAGCTCGTG 
GTGACGCCTA TTTCTCAGGC TCAGGCAAAG CAACGAGCTG GCAGAGCTGG GAGAACAGGC 
CCAGGGAAGT GTTACAGGTT GTACACAGAA CGTGCCTACC GAGATGAAAT GCTGACCACC 
AACGTGCCGG AAATCCAGAG AACCAACTTA GCAAGCACAG TGCTGTCACT CAAGGCCATG 
GGTATCAATG ATCTGCTGTC CTTTGATTTC ATGGATGCCC CACCTATGGA AACTTTGATC 
ACAGCCATGG AGCAGCTGTA CACACTGGGG GCCCTGGATG ACGAGGGCCT GCTCACTCGC 
TTGGGCCGCC GGATGGCAGA GTTCCCTCTG GAGCCAATGC TATGCAAAAT GCTCATCATG 
TCTGTGCATC TGGGCTGCAG TGAGGAAATG CTGACCATTG TATCCATGCT GTCTGTGCAG 
AACGTCTTCT ATAGGCCCAA GGATAAACAA GCCCTTGCAG ATCAGAAGAA GGCCAAATTC 
CACCAGACTG AAGGGGACCA CCTCACCCTG CTAGCTGTGT ACAACTCCTG GAAGAACAAC 
AAGTTCTCCA ACCCATGGTG CTATGAGAAC TTTATCCAGG CTCGTTCCCT GCGCCGGGCC 
CAGGACATTC GCAAGCAGAT GTTAGGCATA ATGGACAGAC ACAAGCTGGA TGTTGTTTCC 
TGTGGCAAGT CCACAGTCCG AGTGCAGAAG GCCATCTGCA GTGGGTTCTT CCGTAATGCT 
GCCAAGAAAG ACCCGCAGGA GGGTTACCGG ACACTGATCG ACCAGCAGGT GGTCTATATC 
CATCCTTCCA GTGCCCTCTT CAACAGACAG CCAGAATGGG TGGTGTACCA TGAGCTGGTG 
CTCACCACCA AGGAATACAT GCGTGAAGTT ACCACCATCG ACCCTCGGTG GCTTGTGGAG 
TTTGCCCCAG CCTTCTTCAA GGTCTCAGAC CCAACTAAGC TAAGCAAACA GAAGAAGCAA 
CAGCGTCTTG AACCCTTGTA CAACCGCTAT GAGGAACCCA ATGCCTGGAG AATATCTCGA 
GCTTTCCGAC GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309145.1
CDS	1081..3918
Translation	MQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	NM_001322216.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322216.1

ORF Insert Sequence:

ATGCAGTTTG GTTGCTTTGT GCAGCTGGAA GGACTAAGGA AGCGGTGGGA AGGCCTGGTG 
CACATCTCTG AGCTCCGGCG GGAGGGTCGT GTGGCCAATG TAGCTGATGT CGTGAGCAAA 
GGCCAGAGGG TCAAAGTCAA AGTGCTGTCC TTCACTGGGA CCAAGACCAG CCTGAGCATG 
AAGGATGTGG ATCAAGAGAC TGGAGAAGAT CTAAACCCAA ATAGACGGCG AAATCTTGTC 
GGGGAGACCA ATGAGGAGAC CTCAATGCGG AATCCTGATA GACCCACTCA CTTGTCCCTT 
GTCAGTGCTC CTGAAGTAGA GGACGACTCA CTGGAACGCA AGCGCCTCAC CCGAATCTCT 
GACCCAGAGA AGTGGGAGAT CAAACAGATG ATTGCTGCCA ATGTCCTTTC CAAAGAAGAA 
TTTCCAGACT TTGATGAAGA GACTGGCATT CTCCCTAAGG TGGATGATGA AGAAGATGAG 
GACCTTGAGA TTGAATTGGT TGAGGAAGAG CCTCCATTCC TGAGAGGGCA CACTAAGCAA 
AGCATGGACA TGAGCCCCAT TAAAATTGTC AAGAACCCAG ACGGCTCCCT CTCCCAAGCA 
GCAATGATGC AGAGTGCCTT GGCCAAAGAA AGGCGGGAAC TCAAACAGGC CCAGCGGGAA 
GCTGAGATGG ATTCTATTCC CATGGGACTC AACAAACACT GGGTTGACCC TCTGCCTGAT 
GCGGAAGGCA GACAGATTGC TGCCAACATG AGGGGTATTG GGATGATGCC CAATGATATT 
CCTGAGTGGA AGAAGCATGC CTTTGGGGGC AACAAAGCCT CTTACGGAAA AAAGACCCAG 
ATGTCAATCC TTGAGCAGAG GGAGAGCCTG CCCATCTACA AACTGAAGGA GCAATTGGTC 
CAGGCCGTCC ATGACAATCA GATCCTGATT GTCATTGGTG AGACAGGATC TGGAAAGACA 
ACACAGATCA CCCAGTACCT GGCGGAGGCA GGCTACACTT CCAGGGGCAA GATTGGGTGT 
ACCCAGCCCA GAAGAGTGGC AGCTATGTCG GTGGCCAAAA GAGTGTCAGA GGAGTTTGGT 
TGTTGCTTAG GCCAAGAGGT GGGCTACACC ATTCGATTTG AGGACTGCAC TAGCCCTGAA 
ACAGTCATCA AGTACATGAC AGATGGGATG TTGCTTAGAG AGTGCTTGAT TGACCCTGAC 
CTCACTCAGT ACGCGATCAT CATGTTGGAC GAGGCACATG AGAGGACAAT TCACACTGAT 
GTGCTCTTTG GATTGTTGAA AAAGACAGTT CAGAAACGGC AGGACATGAA GCTGATTGTC 
ACCTCAGCCA CCTTGGATGC AGTGAAGTTT TCTCAATACT TCTATGAAGC TCCCATTTTC 
ACCATCCCAG GTCGAACATA TCCAGTGGAA ATACTGTACA CAAAGGAACC TGAGACAGAT 
TATCTGGATG CCAGCCTGAT TACTGTTATG CAGATTCATT TAACAGAACC ACCAGGTGAT 
ATCCTGGTCT TCCTGACTGG TCAGGAAGAA ATTGATACTG CTTGTGAGAT CCTGTATGAA 
AGAATGAAAT CCCTGGGACC TGATGTTCCA GAGTTAATTA TCCTCCCAGT GTACTCTGCT 
CTTCCCAGTG AGATGCAGAC CCGAATCTTT GACCCAGCTC CACCAGGCAG CAGAAAGGTT 
GTGATTGCCA CCAATATCGC AGAGACATCG CTGACTATTG ATGGTATCTA CTATGTGGTG 
GACCCAGGAT TCGTGAAACA GAAAGTTTAC AATTCCAAGA CAGGGATTGA CCAGCTCGTG 
GTGACGCCTA TTTCTCAGGC TCAGGCAAAG CAACGAGCTG GCAGAGCTGG GAGAACAGGC 
CCAGGGAAGT GTTACAGGTT GTACACAGAA CGTGCCTACC GAGATGAAAT GCTGACCACC 
AACGTGCCGG AAATCCAGAG AACCAACTTA GCAAGCACAG TGCTGTCACT CAAGGCCATG 
GGTATCAATG ATCTGCTGTC CTTTGATTTC ATGGATGCCC CACCTATGGA AACTTTGATC 
ACAGCCATGG AGCAGCTGTA CACACTGGGG GCCCTGGATG ACGAGGGCCT GCTCACTCGC 
TTGGGCCGCC GGATGGCAGA GTTCCCTCTG GAGCCAATGC TATGCAAAAT GCTCATCATG 
TCTGTGCATC TGGGCTGCAG TGAGGAAATG CTGACCATTG TATCCATGCT GTCTGTGCAG 
AACGTCTTCT ATAGGCCCAA GGATAAACAA GCCCTTGCAG ATCAGAAGAA GGCCAAATTC 
CACCAGACTG AAGGGGACCA CCTCACCCTG CTAGCTGTGT ACAACTCCTG GAAGAACAAC 
AAGTTCTCCA ACCCATGGTG CTATGAGAAC TTTATCCAGG CTCGTTCCCT GCGCCGGGCC 
CAGGACATTC GCAAGCAGAT GTTAGGCATA ATGGACAGAC ACAAGCTGGA TGTTGTTTCC 
TGTGGCAAGT CCACAGTCCG AGTGCAGAAG GCCATCTGCA GTGGGTTCTT CCGTAATGCT 
GCCAAGAAAG ACCCGCAGGA GGGTTACCGG ACACTGATCG ACCAGCAGGT GGTCTATATC 
CATCCTTCCA GTGCCCTCTT CAACAGACAG CCAGAATGGG TGGTGTACCA TGAGCTGGTG 
CTCACCACCA AGGAATACAT GCGTGAAGTT ACCACCATCG ACCCTCGGTG GCTTGTGGAG 
TTTGCCCCAG CCTTCTTCAA GGTCTCAGAC CCAACTAAGC TAAGCAAACA GAAGAAGCAA 
CAGCGTCTTG AACCCTTGTA CAACCGCTAT GAGGAACCCA ATGCCTGGAG AATATCTCGA 
GCTTTCCGAC GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322220.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309149.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 10, mRNA.

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CloneID	OHu101677
Clone ID Related Accession (Same CDS sequence)	NM_001322220.1 , NM_001322220.2
Accession Version	NM_001322220.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.263792.1, SRR1660803.222584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGTATTTCC CAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309149.1
CDS	174..3632
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECISQD

Target ORF information:

RefSeq Version	NM_001322220.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322220.1

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGTATTTCC CAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322220.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309149.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu101677
Clone ID Related Accession (Same CDS sequence)	NM_001322220.1 , NM_001322220.2
Accession Version	NM_001322220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. On May 31, 2019 this sequence version replaced NM_001322220.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.263792.1, SRR1660803.222584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGTATTTCC CAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309149.1
CDS	64..3522
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECISQD

Target ORF information:

RefSeq Version	NM_001322220.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322220.2

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGTATTTCC CAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322218.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309147.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 8, mRNA.

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CloneID	OHu101742
Clone ID Related Accession (Same CDS sequence)	NM_001322218.2 , NM_001322218.1
Accession Version	NM_001322218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. On May 31, 2019 this sequence version replaced NM_001322218.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.171520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGACAAAG AAGAAGAAGC GGAGTCGAAG CCGAGATCGA 
AACCGAGATC GAGACAGAGA TAGGGAACGA AACCGAGATA GAGACCACAA GCGGAGACAC 
CGATCCCGCT CTCGATCACG TTCCAGGACC CGGGAGAGGA ATAAAGTGAA GTCTAGATAT 
CGGTCCAGGA GCAGGAGTCA GAGTCCCCCC AAAGACCGGA AGGACCGGGA CAAATATGGA 
GAGCGGAATC TGGATAGATG GCGGGATAAG CATGTGGACC GCCCTCCTCC AGAAGAGCCC 
ACCATTGGTG ACATTTATAA TGGCAAAGTT ACCAGCATCA TGCAGTTTGG TTGCTTTGTG 
CAGCTGGAAG GACTAAGGAA GCGGTGGGAA GGCCTGGTGC ACATCTCTGA GCTCCGGCGG 
GAGGGTCGTG TGGCCAATGT AGCTGATGTC GTGAGCAAAG GCCAGAGGGT CAAAGTCAAA 
GTGCTGTCCT TCACTGGGAC CAAGACCAGC CTGAGCATGA AGGATGTGGA TCAAGAGACT 
GGAGAAGATC TAAACCCAAA TAGACGGCGA AATCTTGTCG GGGAGACCAA TGAGGAGACC 
TCAATGCGGA ATCCTGATAG ACCCACTCAC TTGTCCCTTG TCAGTGCTCC TGAAGTAGAG 
GACGACTCAC TGGAACGCAA GCGCCTCACC CGAATCTCTG ACCCAGAGAA GTGGGAGATC 
AAACAGATGA TTGCTGCCAA TGTCCTTTCC AAAGAAGAAT TTCCAGACTT TGATGAAGAG 
ACTGGCATTC TCCCTAAGGT GGATGATGAA GAAGATGAGG ACCTTGAGAT TGAATTGGTT 
GAGGAAGAGC CTCCATTCCT GAGAGGGCAC ACTAAGCAAA GCATGGACAT GAGCCCCATT 
AAAATTGTCA AGAACCCAGA CGGCTCCCTC TCCCAAGCAG CAATGATGCA GAGTGCCTTG 
GCCAAAGAAA GGCGGGAACT CAAACAGGCC CAGCGGGAAG CTGAGATGGA TTCTATTCCC 
ATGGGACTCA ACAAACACTG GGTTGACCCT CTGCCTGATG CGGAAGGCAG ACAGATTGCT 
GCCAACATGA GGGGTATTGG GATGATGCCC AATGATATTC CTGAGTGGAA GAAGCATGCC 
TTTGGGGGCA ACAAAGCCTC TTACGGAAAA AAGACCCAGA TGTCAATCCT TGAGCAGAGG 
GAGAGCCTGC CCATCTACAA ACTGAAGGAG CAATTGGTCC AGGCCGTCCA TGACAATCAG 
ATCCTGATTG TCATTGGTGA GACAGGATCT GGAAAGACAA CACAGATCAC CCAGTACCTG 
GCGGAGGCAG GCTACACTTC CAGGGGCAAG ATTGGGTGTA CCCAGCCCAG AAGAGTGGCA 
GCTATGTCGG TGGCCAAAAG AGTGTCAGAG GAGTTTGGTT GTTGCTTAGG CCAAGAGGTG 
GGCTACACCA TTCGATTTGA GGACTGCACT AGCCCTGAAA CAGTCATCAA GTACATGACA 
GATGGGATGT TGCTTAGAGA GTGCTTGATT GACCCTGACC TCACTCAGTA CGCGATCATC 
ATGTTGGACG AGGCACATGA GAGGACAATT CACACTGATG TGCTCTTTGG ATTGTTGAAA 
AAGACAGTTC AGAAACGGCA GGACATGAAG CTGATTGTCA CCTCAGCCAC CTTGGATGCA 
GTGAAGTTTT CTCAATACTT CTATGAAGCT CCCATTTTCA CCATCCCAGG TCGAACATAT 
CCAGTGGAAA TACTGTACAC AAAGGAACCT GAGACAGATT ATCTGGATGC CAGCCTGATT 
ACTGTTATGC AGATTCATTT AACAGAACCA CCAGGTGATA TCCTGGTCTT CCTGACTGGT 
CAGGAAGAAA TTGATACTGC TTGTGAGATC CTGTATGAAA GAATGAAATC CCTGGGACCT 
GATGTTCCAG AGTTAATTAT CCTCCCAGTG TACTCTGCTC TTCCCAGTGA GATGCAGACC 
CGAATCTTTG ACCCAGCTCC ACCAGGCAGC AGAAAGGTTG TGATTGCCAC CAATATCGCA 
GAGACATCGC TGACTATTGA TGGTATCTAC TATGTGGTGG ACCCAGGATT CGTGAAACAG 
AAAGTTTACA ATTCCAAGAC AGGGATTGAC CAGCTCGTGG TGACGCCTAT TTCTCAGGCT 
CAGGCAAAGC AACGAGCTGG CAGAGCTGGG AGAACAGGCC CAGGGAAGTG TTACAGGTTG 
TACACAGAAC GTGCCTACCG AGATGAAATG CTGACCACCA ACGTGCCGGA AATCCAGAGA 
ACCAACTTAG CAAGCACAGT GCTGTCACTC AAGGCCATGG GTATCAATGA TCTGCTGTCC 
TTTGATTTCA TGGATGCCCC ACCTATGGAA ACTTTGATCA CAGCCATGGA GCAGCTGTAC 
ACACTGGGGG CCCTGGATGA CGAGGGCCTG CTCACTCGCT TGGGCCGCCG GATGGCAGAG 
TTCCCTCTGG AGCCAATGCT ATGCAAAATG CTCATCATGT CTGTGCATCT GGGCTGCAGT 
GAGGAAATGC TGACCATTGT ATCCATGCTG TCTGTGCAGA ACGTCTTCTA TAGGCCCAAG 
GATAAACAAG CCCTTGCAGA TCAGAAGAAG GCCAAATTCC ACCAGACTGA AGGGGACCAC 
CTCACCCTGC TAGCTGTGTA CAACTCCTGG AAGAACAACA AGTTCTCCAA CCCATGGTGC 
TATGAGAACT TTATCCAGGC TCGTTCCCTG CGCCGGGCCC AGGACATTCG CAAGCAGATG 
TTAGGCATAA TGGACAGACA CAAGCTGGAT GTTGTTTCCT GTGGCAAGTC CACAGTCCGA 
GTGCAGAAGG CCATCTGCAG TGGGTTCTTC CGTAATGCTG CCAAGAAAGA CCCGCAGGAG 
GGTTACCGGA CACTGATCGA CCAGCAGGTG GTCTATATCC ATCCTTCCAG TGCCCTCTTC 
AACAGACAGC CAGAATGCCC AAAGCACTTC CTCCCTGTGG TTGCTGTAGC TGTCTCATTG 
GAGCAGTGCC TCTCCAAGTT TGAGGATTTG AACAAAGAAC TGGGACTGGT GACTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309147.1
CDS	64..3603
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECPKHFLPVVAVAVSLEQCLSKFEDLNKELGLVTC

Target ORF information:

RefSeq Version	NM_001322218.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322218.2

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGACAAAG AAGAAGAAGC GGAGTCGAAG CCGAGATCGA 
AACCGAGATC GAGACAGAGA TAGGGAACGA AACCGAGATA GAGACCACAA GCGGAGACAC 
CGATCCCGCT CTCGATCACG TTCCAGGACC CGGGAGAGGA ATAAAGTGAA GTCTAGATAT 
CGGTCCAGGA GCAGGAGTCA GAGTCCCCCC AAAGACCGGA AGGACCGGGA CAAATATGGA 
GAGCGGAATC TGGATAGATG GCGGGATAAG CATGTGGACC GCCCTCCTCC AGAAGAGCCC 
ACCATTGGTG ACATTTATAA TGGCAAAGTT ACCAGCATCA TGCAGTTTGG TTGCTTTGTG 
CAGCTGGAAG GACTAAGGAA GCGGTGGGAA GGCCTGGTGC ACATCTCTGA GCTCCGGCGG 
GAGGGTCGTG TGGCCAATGT AGCTGATGTC GTGAGCAAAG GCCAGAGGGT CAAAGTCAAA 
GTGCTGTCCT TCACTGGGAC CAAGACCAGC CTGAGCATGA AGGATGTGGA TCAAGAGACT 
GGAGAAGATC TAAACCCAAA TAGACGGCGA AATCTTGTCG GGGAGACCAA TGAGGAGACC 
TCAATGCGGA ATCCTGATAG ACCCACTCAC TTGTCCCTTG TCAGTGCTCC TGAAGTAGAG 
GACGACTCAC TGGAACGCAA GCGCCTCACC CGAATCTCTG ACCCAGAGAA GTGGGAGATC 
AAACAGATGA TTGCTGCCAA TGTCCTTTCC AAAGAAGAAT TTCCAGACTT TGATGAAGAG 
ACTGGCATTC TCCCTAAGGT GGATGATGAA GAAGATGAGG ACCTTGAGAT TGAATTGGTT 
GAGGAAGAGC CTCCATTCCT GAGAGGGCAC ACTAAGCAAA GCATGGACAT GAGCCCCATT 
AAAATTGTCA AGAACCCAGA CGGCTCCCTC TCCCAAGCAG CAATGATGCA GAGTGCCTTG 
GCCAAAGAAA GGCGGGAACT CAAACAGGCC CAGCGGGAAG CTGAGATGGA TTCTATTCCC 
ATGGGACTCA ACAAACACTG GGTTGACCCT CTGCCTGATG CGGAAGGCAG ACAGATTGCT 
GCCAACATGA GGGGTATTGG GATGATGCCC AATGATATTC CTGAGTGGAA GAAGCATGCC 
TTTGGGGGCA ACAAAGCCTC TTACGGAAAA AAGACCCAGA TGTCAATCCT TGAGCAGAGG 
GAGAGCCTGC CCATCTACAA ACTGAAGGAG CAATTGGTCC AGGCCGTCCA TGACAATCAG 
ATCCTGATTG TCATTGGTGA GACAGGATCT GGAAAGACAA CACAGATCAC CCAGTACCTG 
GCGGAGGCAG GCTACACTTC CAGGGGCAAG ATTGGGTGTA CCCAGCCCAG AAGAGTGGCA 
GCTATGTCGG TGGCCAAAAG AGTGTCAGAG GAGTTTGGTT GTTGCTTAGG CCAAGAGGTG 
GGCTACACCA TTCGATTTGA GGACTGCACT AGCCCTGAAA CAGTCATCAA GTACATGACA 
GATGGGATGT TGCTTAGAGA GTGCTTGATT GACCCTGACC TCACTCAGTA CGCGATCATC 
ATGTTGGACG AGGCACATGA GAGGACAATT CACACTGATG TGCTCTTTGG ATTGTTGAAA 
AAGACAGTTC AGAAACGGCA GGACATGAAG CTGATTGTCA CCTCAGCCAC CTTGGATGCA 
GTGAAGTTTT CTCAATACTT CTATGAAGCT CCCATTTTCA CCATCCCAGG TCGAACATAT 
CCAGTGGAAA TACTGTACAC AAAGGAACCT GAGACAGATT ATCTGGATGC CAGCCTGATT 
ACTGTTATGC AGATTCATTT AACAGAACCA CCAGGTGATA TCCTGGTCTT CCTGACTGGT 
CAGGAAGAAA TTGATACTGC TTGTGAGATC CTGTATGAAA GAATGAAATC CCTGGGACCT 
GATGTTCCAG AGTTAATTAT CCTCCCAGTG TACTCTGCTC TTCCCAGTGA GATGCAGACC 
CGAATCTTTG ACCCAGCTCC ACCAGGCAGC AGAAAGGTTG TGATTGCCAC CAATATCGCA 
GAGACATCGC TGACTATTGA TGGTATCTAC TATGTGGTGG ACCCAGGATT CGTGAAACAG 
AAAGTTTACA ATTCCAAGAC AGGGATTGAC CAGCTCGTGG TGACGCCTAT TTCTCAGGCT 
CAGGCAAAGC AACGAGCTGG CAGAGCTGGG AGAACAGGCC CAGGGAAGTG TTACAGGTTG 
TACACAGAAC GTGCCTACCG AGATGAAATG CTGACCACCA ACGTGCCGGA AATCCAGAGA 
ACCAACTTAG CAAGCACAGT GCTGTCACTC AAGGCCATGG GTATCAATGA TCTGCTGTCC 
TTTGATTTCA TGGATGCCCC ACCTATGGAA ACTTTGATCA CAGCCATGGA GCAGCTGTAC 
ACACTGGGGG CCCTGGATGA CGAGGGCCTG CTCACTCGCT TGGGCCGCCG GATGGCAGAG 
TTCCCTCTGG AGCCAATGCT ATGCAAAATG CTCATCATGT CTGTGCATCT GGGCTGCAGT 
GAGGAAATGC TGACCATTGT ATCCATGCTG TCTGTGCAGA ACGTCTTCTA TAGGCCCAAG 
GATAAACAAG CCCTTGCAGA TCAGAAGAAG GCCAAATTCC ACCAGACTGA AGGGGACCAC 
CTCACCCTGC TAGCTGTGTA CAACTCCTGG AAGAACAACA AGTTCTCCAA CCCATGGTGC 
TATGAGAACT TTATCCAGGC TCGTTCCCTG CGCCGGGCCC AGGACATTCG CAAGCAGATG 
TTAGGCATAA TGGACAGACA CAAGCTGGAT GTTGTTTCCT GTGGCAAGTC CACAGTCCGA 
GTGCAGAAGG CCATCTGCAG TGGGTTCTTC CGTAATGCTG CCAAGAAAGA CCCGCAGGAG 
GGTTACCGGA CACTGATCGA CCAGCAGGTG GTCTATATCC ATCCTTCCAG TGCCCTCTTC 
AACAGACAGC CAGAATGCCC AAAGCACTTC CTCCCTGTGG TTGCTGTAGC TGTCTCATTG 
GAGCAGTGCC TCTCCAAGTT TGAGGATTTG AACAAAGAAC TGGGACTGGT GACTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322218.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309147.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 8, mRNA.

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CloneID	OHu101742
Clone ID Related Accession (Same CDS sequence)	NM_001322218.2 , NM_001322218.1
Accession Version	NM_001322218.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.171520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGACAAAG AAGAAGAAGC GGAGTCGAAG CCGAGATCGA 
AACCGAGATC GAGACAGAGA TAGGGAACGA AACCGAGATA GAGACCACAA GCGGAGACAC 
CGATCCCGCT CTCGATCACG TTCCAGGACC CGGGAGAGGA ATAAAGTGAA GTCTAGATAT 
CGGTCCAGGA GCAGGAGTCA GAGTCCCCCC AAAGACCGGA AGGACCGGGA CAAATATGGA 
GAGCGGAATC TGGATAGATG GCGGGATAAG CATGTGGACC GCCCTCCTCC AGAAGAGCCC 
ACCATTGGTG ACATTTATAA TGGCAAAGTT ACCAGCATCA TGCAGTTTGG TTGCTTTGTG 
CAGCTGGAAG GACTAAGGAA GCGGTGGGAA GGCCTGGTGC ACATCTCTGA GCTCCGGCGG 
GAGGGTCGTG TGGCCAATGT AGCTGATGTC GTGAGCAAAG GCCAGAGGGT CAAAGTCAAA 
GTGCTGTCCT TCACTGGGAC CAAGACCAGC CTGAGCATGA AGGATGTGGA TCAAGAGACT 
GGAGAAGATC TAAACCCAAA TAGACGGCGA AATCTTGTCG GGGAGACCAA TGAGGAGACC 
TCAATGCGGA ATCCTGATAG ACCCACTCAC TTGTCCCTTG TCAGTGCTCC TGAAGTAGAG 
GACGACTCAC TGGAACGCAA GCGCCTCACC CGAATCTCTG ACCCAGAGAA GTGGGAGATC 
AAACAGATGA TTGCTGCCAA TGTCCTTTCC AAAGAAGAAT TTCCAGACTT TGATGAAGAG 
ACTGGCATTC TCCCTAAGGT GGATGATGAA GAAGATGAGG ACCTTGAGAT TGAATTGGTT 
GAGGAAGAGC CTCCATTCCT GAGAGGGCAC ACTAAGCAAA GCATGGACAT GAGCCCCATT 
AAAATTGTCA AGAACCCAGA CGGCTCCCTC TCCCAAGCAG CAATGATGCA GAGTGCCTTG 
GCCAAAGAAA GGCGGGAACT CAAACAGGCC CAGCGGGAAG CTGAGATGGA TTCTATTCCC 
ATGGGACTCA ACAAACACTG GGTTGACCCT CTGCCTGATG CGGAAGGCAG ACAGATTGCT 
GCCAACATGA GGGGTATTGG GATGATGCCC AATGATATTC CTGAGTGGAA GAAGCATGCC 
TTTGGGGGCA ACAAAGCCTC TTACGGAAAA AAGACCCAGA TGTCAATCCT TGAGCAGAGG 
GAGAGCCTGC CCATCTACAA ACTGAAGGAG CAATTGGTCC AGGCCGTCCA TGACAATCAG 
ATCCTGATTG TCATTGGTGA GACAGGATCT GGAAAGACAA CACAGATCAC CCAGTACCTG 
GCGGAGGCAG GCTACACTTC CAGGGGCAAG ATTGGGTGTA CCCAGCCCAG AAGAGTGGCA 
GCTATGTCGG TGGCCAAAAG AGTGTCAGAG GAGTTTGGTT GTTGCTTAGG CCAAGAGGTG 
GGCTACACCA TTCGATTTGA GGACTGCACT AGCCCTGAAA CAGTCATCAA GTACATGACA 
GATGGGATGT TGCTTAGAGA GTGCTTGATT GACCCTGACC TCACTCAGTA CGCGATCATC 
ATGTTGGACG AGGCACATGA GAGGACAATT CACACTGATG TGCTCTTTGG ATTGTTGAAA 
AAGACAGTTC AGAAACGGCA GGACATGAAG CTGATTGTCA CCTCAGCCAC CTTGGATGCA 
GTGAAGTTTT CTCAATACTT CTATGAAGCT CCCATTTTCA CCATCCCAGG TCGAACATAT 
CCAGTGGAAA TACTGTACAC AAAGGAACCT GAGACAGATT ATCTGGATGC CAGCCTGATT 
ACTGTTATGC AGATTCATTT AACAGAACCA CCAGGTGATA TCCTGGTCTT CCTGACTGGT 
CAGGAAGAAA TTGATACTGC TTGTGAGATC CTGTATGAAA GAATGAAATC CCTGGGACCT 
GATGTTCCAG AGTTAATTAT CCTCCCAGTG TACTCTGCTC TTCCCAGTGA GATGCAGACC 
CGAATCTTTG ACCCAGCTCC ACCAGGCAGC AGAAAGGTTG TGATTGCCAC CAATATCGCA 
GAGACATCGC TGACTATTGA TGGTATCTAC TATGTGGTGG ACCCAGGATT CGTGAAACAG 
AAAGTTTACA ATTCCAAGAC AGGGATTGAC CAGCTCGTGG TGACGCCTAT TTCTCAGGCT 
CAGGCAAAGC AACGAGCTGG CAGAGCTGGG AGAACAGGCC CAGGGAAGTG TTACAGGTTG 
TACACAGAAC GTGCCTACCG AGATGAAATG CTGACCACCA ACGTGCCGGA AATCCAGAGA 
ACCAACTTAG CAAGCACAGT GCTGTCACTC AAGGCCATGG GTATCAATGA TCTGCTGTCC 
TTTGATTTCA TGGATGCCCC ACCTATGGAA ACTTTGATCA CAGCCATGGA GCAGCTGTAC 
ACACTGGGGG CCCTGGATGA CGAGGGCCTG CTCACTCGCT TGGGCCGCCG GATGGCAGAG 
TTCCCTCTGG AGCCAATGCT ATGCAAAATG CTCATCATGT CTGTGCATCT GGGCTGCAGT 
GAGGAAATGC TGACCATTGT ATCCATGCTG TCTGTGCAGA ACGTCTTCTA TAGGCCCAAG 
GATAAACAAG CCCTTGCAGA TCAGAAGAAG GCCAAATTCC ACCAGACTGA AGGGGACCAC 
CTCACCCTGC TAGCTGTGTA CAACTCCTGG AAGAACAACA AGTTCTCCAA CCCATGGTGC 
TATGAGAACT TTATCCAGGC TCGTTCCCTG CGCCGGGCCC AGGACATTCG CAAGCAGATG 
TTAGGCATAA TGGACAGACA CAAGCTGGAT GTTGTTTCCT GTGGCAAGTC CACAGTCCGA 
GTGCAGAAGG CCATCTGCAG TGGGTTCTTC CGTAATGCTG CCAAGAAAGA CCCGCAGGAG 
GGTTACCGGA CACTGATCGA CCAGCAGGTG GTCTATATCC ATCCTTCCAG TGCCCTCTTC 
AACAGACAGC CAGAATGCCC AAAGCACTTC CTCCCTGTGG TTGCTGTAGC TGTCTCATTG 
GAGCAGTGCC TCTCCAAGTT TGAGGATTTG AACAAAGAAC TGGGACTGGT GACTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309147.1
CDS	174..3713
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECPKHFLPVVAVAVSLEQCLSKFEDLNKELGLVTC

Target ORF information:

RefSeq Version	NM_001322218.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322218.1

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGACAAAG AAGAAGAAGC GGAGTCGAAG CCGAGATCGA 
AACCGAGATC GAGACAGAGA TAGGGAACGA AACCGAGATA GAGACCACAA GCGGAGACAC 
CGATCCCGCT CTCGATCACG TTCCAGGACC CGGGAGAGGA ATAAAGTGAA GTCTAGATAT 
CGGTCCAGGA GCAGGAGTCA GAGTCCCCCC AAAGACCGGA AGGACCGGGA CAAATATGGA 
GAGCGGAATC TGGATAGATG GCGGGATAAG CATGTGGACC GCCCTCCTCC AGAAGAGCCC 
ACCATTGGTG ACATTTATAA TGGCAAAGTT ACCAGCATCA TGCAGTTTGG TTGCTTTGTG 
CAGCTGGAAG GACTAAGGAA GCGGTGGGAA GGCCTGGTGC ACATCTCTGA GCTCCGGCGG 
GAGGGTCGTG TGGCCAATGT AGCTGATGTC GTGAGCAAAG GCCAGAGGGT CAAAGTCAAA 
GTGCTGTCCT TCACTGGGAC CAAGACCAGC CTGAGCATGA AGGATGTGGA TCAAGAGACT 
GGAGAAGATC TAAACCCAAA TAGACGGCGA AATCTTGTCG GGGAGACCAA TGAGGAGACC 
TCAATGCGGA ATCCTGATAG ACCCACTCAC TTGTCCCTTG TCAGTGCTCC TGAAGTAGAG 
GACGACTCAC TGGAACGCAA GCGCCTCACC CGAATCTCTG ACCCAGAGAA GTGGGAGATC 
AAACAGATGA TTGCTGCCAA TGTCCTTTCC AAAGAAGAAT TTCCAGACTT TGATGAAGAG 
ACTGGCATTC TCCCTAAGGT GGATGATGAA GAAGATGAGG ACCTTGAGAT TGAATTGGTT 
GAGGAAGAGC CTCCATTCCT GAGAGGGCAC ACTAAGCAAA GCATGGACAT GAGCCCCATT 
AAAATTGTCA AGAACCCAGA CGGCTCCCTC TCCCAAGCAG CAATGATGCA GAGTGCCTTG 
GCCAAAGAAA GGCGGGAACT CAAACAGGCC CAGCGGGAAG CTGAGATGGA TTCTATTCCC 
ATGGGACTCA ACAAACACTG GGTTGACCCT CTGCCTGATG CGGAAGGCAG ACAGATTGCT 
GCCAACATGA GGGGTATTGG GATGATGCCC AATGATATTC CTGAGTGGAA GAAGCATGCC 
TTTGGGGGCA ACAAAGCCTC TTACGGAAAA AAGACCCAGA TGTCAATCCT TGAGCAGAGG 
GAGAGCCTGC CCATCTACAA ACTGAAGGAG CAATTGGTCC AGGCCGTCCA TGACAATCAG 
ATCCTGATTG TCATTGGTGA GACAGGATCT GGAAAGACAA CACAGATCAC CCAGTACCTG 
GCGGAGGCAG GCTACACTTC CAGGGGCAAG ATTGGGTGTA CCCAGCCCAG AAGAGTGGCA 
GCTATGTCGG TGGCCAAAAG AGTGTCAGAG GAGTTTGGTT GTTGCTTAGG CCAAGAGGTG 
GGCTACACCA TTCGATTTGA GGACTGCACT AGCCCTGAAA CAGTCATCAA GTACATGACA 
GATGGGATGT TGCTTAGAGA GTGCTTGATT GACCCTGACC TCACTCAGTA CGCGATCATC 
ATGTTGGACG AGGCACATGA GAGGACAATT CACACTGATG TGCTCTTTGG ATTGTTGAAA 
AAGACAGTTC AGAAACGGCA GGACATGAAG CTGATTGTCA CCTCAGCCAC CTTGGATGCA 
GTGAAGTTTT CTCAATACTT CTATGAAGCT CCCATTTTCA CCATCCCAGG TCGAACATAT 
CCAGTGGAAA TACTGTACAC AAAGGAACCT GAGACAGATT ATCTGGATGC CAGCCTGATT 
ACTGTTATGC AGATTCATTT AACAGAACCA CCAGGTGATA TCCTGGTCTT CCTGACTGGT 
CAGGAAGAAA TTGATACTGC TTGTGAGATC CTGTATGAAA GAATGAAATC CCTGGGACCT 
GATGTTCCAG AGTTAATTAT CCTCCCAGTG TACTCTGCTC TTCCCAGTGA GATGCAGACC 
CGAATCTTTG ACCCAGCTCC ACCAGGCAGC AGAAAGGTTG TGATTGCCAC CAATATCGCA 
GAGACATCGC TGACTATTGA TGGTATCTAC TATGTGGTGG ACCCAGGATT CGTGAAACAG 
AAAGTTTACA ATTCCAAGAC AGGGATTGAC CAGCTCGTGG TGACGCCTAT TTCTCAGGCT 
CAGGCAAAGC AACGAGCTGG CAGAGCTGGG AGAACAGGCC CAGGGAAGTG TTACAGGTTG 
TACACAGAAC GTGCCTACCG AGATGAAATG CTGACCACCA ACGTGCCGGA AATCCAGAGA 
ACCAACTTAG CAAGCACAGT GCTGTCACTC AAGGCCATGG GTATCAATGA TCTGCTGTCC 
TTTGATTTCA TGGATGCCCC ACCTATGGAA ACTTTGATCA CAGCCATGGA GCAGCTGTAC 
ACACTGGGGG CCCTGGATGA CGAGGGCCTG CTCACTCGCT TGGGCCGCCG GATGGCAGAG 
TTCCCTCTGG AGCCAATGCT ATGCAAAATG CTCATCATGT CTGTGCATCT GGGCTGCAGT 
GAGGAAATGC TGACCATTGT ATCCATGCTG TCTGTGCAGA ACGTCTTCTA TAGGCCCAAG 
GATAAACAAG CCCTTGCAGA TCAGAAGAAG GCCAAATTCC ACCAGACTGA AGGGGACCAC 
CTCACCCTGC TAGCTGTGTA CAACTCCTGG AAGAACAACA AGTTCTCCAA CCCATGGTGC 
TATGAGAACT TTATCCAGGC TCGTTCCCTG CGCCGGGCCC AGGACATTCG CAAGCAGATG 
TTAGGCATAA TGGACAGACA CAAGCTGGAT GTTGTTTCCT GTGGCAAGTC CACAGTCCGA 
GTGCAGAAGG CCATCTGCAG TGGGTTCTTC CGTAATGCTG CCAAGAAAGA CCCGCAGGAG 
GGTTACCGGA CACTGATCGA CCAGCAGGTG GTCTATATCC ATCCTTCCAG TGCCCTCTTC 
AACAGACAGC CAGAATGCCC AAAGCACTTC CTCCCTGTGG TTGCTGTAGC TGTCTCATTG 
GAGCAGTGCC TCTCCAAGTT TGAGGATTTG AACAAAGAAC TGGGACTGGT GACTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322219.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309148.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu101754
Clone ID Related Accession (Same CDS sequence)	NM_001322219.1 , NM_001322219.2
Accession Version	NM_001322219.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.235278.1, SRR1660807.109781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGACCTC TACTCACGGT TTTCTGAATG GAAATCAGGA 
ACAAACTGCT CATCACTGTC TGGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309148.1
CDS	174..3683
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWDLYSRFSEWKSGTNCSSLSGT

Target ORF information:

RefSeq Version	NM_001322219.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322219.1

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGACCTC TACTCACGGT TTTCTGAATG GAAATCAGGA 
ACAAACTGCT CATCACTGTC TGGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322219.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309148.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 9, mRNA.

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CloneID	OHu101754
Clone ID Related Accession (Same CDS sequence)	NM_001322219.1 , NM_001322219.2
Accession Version	NM_001322219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. On May 31, 2019 this sequence version replaced NM_001322219.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.235278.1, SRR1803617.214026.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGACCTC TACTCACGGT TTTCTGAATG GAAATCAGGA 
ACAAACTGCT CATCACTGTC TGGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309148.1
CDS	64..3573
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWDLYSRFSEWKSGTNCSSLSGT

Target ORF information:

RefSeq Version	NM_001322219.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322219.2

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGACCTC TACTCACGGT TTTCTGAATG GAAATCAGGA 
ACAAACTGCT CATCACTGTC TGGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322217.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309146.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 7, mRNA.

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CloneID	OHu101781
Clone ID Related Accession (Same CDS sequence)	NM_001322217.1
Accession Version	NM_001322217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.143481.1, SRR1660809.82247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCTC CAGCGAAGCC TTCCACTAGC AAAGATCCAG TTGTTAAACC TAAAACAGAA 
AAAGAAAAGC TGAAGGAACT CTTCCCAGTC CTTTGCCAAC CGGACAACCC TTCTGTTCGG 
ACCATGTTGG ATGAAGATGA TGTGAAAGTT GCTGTGGATG TCCTGAAAGA ACTGGAAGCT 
TTAATGCCCA GCGCAGCAGG CCAGGAGAAG CAAAGAGATG CTGAACACCG GGACAGGACA 
AAGAAGAAGA AGCGGAGTCG AAGCCGAGAT CGAAACCGAG ATCGAGACAG AGATAGGGAA 
CGAAACCGAG ATAGAGACCA CAAGCGGAGA CACCGATCCC GCTCTCGATC ACGTTCCAGG 
ACCCGGGAGA GGAATAAAGT GAAGTCTAGA TATCGGTCCA GGAGCAGGAG TCAGAGTCCC 
CCCAAAGACC GGAAGGACCG GGACAAATAT GGAGAGCGGA ATCTGGATAG ATGGCGGGAT 
AAGCATGTGG ACCGCCCTCC TCCAGAAGAG CCCACCATTG GTGACATTTA TAATGGCAAA 
GTTACCAGCA TCATGCAGTT TGGTTGCTTT GTGCAGCTGG AAGGACTAAG GAAGCGGTGG 
GAAGGCCTGG TGCACATCTC TGAGCTCCGG CGGGAGGGTC GTGTGGCCAA TGTAGCTGAT 
GTCGTGAGCA AAGGCCAGAG GGTCAAAGTC AAAGTGCTGT CCTTCACTGG GACCAAGACC 
AGCCTGAGCA TGAAGGATGT GGATCAAGAG ACTGGAGAAG ATCTAAACCC AAATAGACGG 
CGAAATCTTG TCGGGGAGAC CAATGAGGAG ACCTCAATGC GGAATCCTGA TAGACCCACT 
CACTTGTCCC TTGTCAGTGC TCCTGAAGTA GAGGACGACT CACTGGAACG CAAGCGCCTC 
ACCCGAATCT CTGACCCAGA GAAGTGGGAG ATCAAACAGA TGATTGCTGC CAATGTCCTT 
TCCAAAGAAG AATTTCCAGA CTTTGATGAA GAGACTGGCA TTCTCCCTAA GGTGGATGAT 
GAAGAAGATG AGGACCTTGA GATTGAATTG GTTGAGGAAG AGCCTCCATT CCTGAGAGGG 
CACACTAAGC AAAGCATGGA CATGAGCCCC ATTAAAATTG TCAAGAACCC AGACGGCTCC 
CTCTCCCAAG CAGCAATGAT GCAGAGTGCC TTGGCCAAAG AAAGGCGGGA ACTCAAACAG 
GCCCAGCGGG AAGCTGAGAT GGATTCTATT CCCATGGGAC TCAACAAACA CTGGGTTGAC 
CCTCTGCCTG ATGCGGAAGG CAGACAGATT GCTGCCAACA TGAGGGGTAT TGGGATGATG 
CCCAATGATA TTCCTGAGTG GAAGAAGCAT GCCTTTGGGG GCAACAAAGC CTCTTACGGA 
AAAAAGACCC AGATGTCAAT CCTTGAGCAG AGGGAGAGCC TGCCCATCTA CAAACTGAAG 
GAGCAATTGG TCCAGGCCGT CCATGACAAT CAGATCCTGA TTGTCATTGG TGAGACAGGA 
TCTGGAAAGA CAACACAGAT CACCCAGTAC CTGGCGGAGG CAGGCTACAC TTCCAGGGGC 
AAGATTGGGT GTACCCAGCC CAGAAGAGTG GCAGCTATGT CGGTGGCCAA AAGAGTGTCA 
GAGGAGTTTG GTTGTTGCTT AGGCCAAGAG GTGGGCTACA CCATTCGATT TGAGGACTGC 
ACTAGCCCTG AAACAGTCAT CAAGTACATG ACAGATGGGA TGTTGCTTAG AGAGTGCTTG 
ATTGACCCTG ACCTCACTCA GTACGCGATC ATCATGTTGG ACGAGGCACA TGAGAGGACA 
ATTCACACTG ATGTGCTCTT TGGATTGTTG AAAAAGACAG TTCAGAAACG GCAGGACATG 
AAGCTGATTG TCACCTCAGC CACCTTGGAT GCAGTGAAGT TTTCTCAATA CTTCTATGAA 
GCTCCCATTT TCACCATCCC AGGTCGAACA TATCCAGTGG AAATACTGTA CACAAAGGAA 
CCTGAGACAG ATTATCTGGA TGCCAGCCTG ATTACTGTTA TGCAGATTCA TTTAACAGAA 
CCACCAGGTG ATATCCTGGT CTTCCTGACT GGTCAGGAAG AAATTGATAC TGCTTGTGAG 
ATCCTGTATG AAAGAATGAA ATCCCTGGGA CCTGATGTTC CAGAGTTAAT TATCCTCCCA 
GTGTACTCTG CTCTTCCCAG TGAGATGCAG ACCCGAATCT TTGACCCAGC TCCACCAGGC 
AGCAGAAAGG TTGTGATTGC CACCAATATC GCAGAGACAT CGCTGACTAT TGATGGTATC 
TACTATGTGG TGGACCCAGG ATTCGTGAAA CAGAAAGTTT ACAATTCCAA GACAGGGATT 
GACCAGCTCG TGGTGACGCC TATTTCTCAG GCTCAGGCAA AGCAACGAGC TGGCAGAGCT 
GGGAGAACAG GCCCAGGGAA GTGTTACAGG TTGTACACAG AACGTGCCTA CCGAGATGAA 
ATGCTGACCA CCAACGTGCC GGAAATCCAG AGAACCAACT TAGCAAGCAC AGTGCTGTCA 
CTCAAGGCCA TGGGTATCAA TGATCTGCTG TCCTTTGATT TCATGGATGC CCCACCTATG 
GAAACTTTGA TCACAGCCAT GGAGCAGCTG TACACACTGG GGGCCCTGGA TGACGAGGGC 
CTGCTCACTC GCTTGGGCCG CCGGATGGCA GAGTTCCCTC TGGAGCCAAT GCTATGCAAA 
ATGCTCATCA TGTCTGTGCA TCTGGGCTGC AGTGAGGAAA TGCTGACCAT TGTATCCATG 
CTGTCTGTGC AGAACGTCTT CTATAGGCCC AAGGATAAAC AAGCCCTTGC AGATCAGAAG 
AAGGCCAAAT TCCACCAGAC TGAAGGGGAC CACCTCACCC TGCTAGCTGT GTACAACTCC 
TGGAAGAACA ACAAGTTCTC CAACCCATGG TGCTATGAGA ACTTTATCCA GGCTCGTTCC 
CTGCGCCGGG CCCAGGACAT TCGCAAGCAG ATGTTAGGCA TAATGGACAG ACACAAGCTG 
GATGTTGTTT CCTGTGGCAA GTCCACAGTC CGAGTGCAGA AGGCCATCTG CAGTGGGTTC 
TTCCGTAATG CTGCCAAGAA AGACCCGCAG GAGGGTTACC GGACACTGAT CGACCAGCAG 
GTGGTCTATA TCCATCCTTC CAGTGCCCTC TTCAACAGAC AGCCAGAATG GGTGGTGTAC 
CATGAGCTGG TGCTCACCAC CAAGGAATAC ATGCGTGAAG TTACCACCAT CGACCCTCGG 
TGGCTTGTGG AGTTTGCCCC AGCCTTCTTC AAGGTCTCAG ACCCAACTAA GCTAAGCAAA 
CAGAAGAAGC AACAGCGTCT TGAACCCTTG TACAACCGCT ATGAGGAACC CAATGCCTGG 
AGAATATCTC GAGCTTTCCG ACGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309146.1
CDS	361..3750
Translation	MRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	NM_001322217.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322217.1

ORF Insert Sequence:

ATGCGGCCTC CAGCGAAGCC TTCCACTAGC AAAGATCCAG TTGTTAAACC TAAAACAGAA 
AAAGAAAAGC TGAAGGAACT CTTCCCAGTC CTTTGCCAAC CGGACAACCC TTCTGTTCGG 
ACCATGTTGG ATGAAGATGA TGTGAAAGTT GCTGTGGATG TCCTGAAAGA ACTGGAAGCT 
TTAATGCCCA GCGCAGCAGG CCAGGAGAAG CAAAGAGATG CTGAACACCG GGACAGGACA 
AAGAAGAAGA AGCGGAGTCG AAGCCGAGAT CGAAACCGAG ATCGAGACAG AGATAGGGAA 
CGAAACCGAG ATAGAGACCA CAAGCGGAGA CACCGATCCC GCTCTCGATC ACGTTCCAGG 
ACCCGGGAGA GGAATAAAGT GAAGTCTAGA TATCGGTCCA GGAGCAGGAG TCAGAGTCCC 
CCCAAAGACC GGAAGGACCG GGACAAATAT GGAGAGCGGA ATCTGGATAG ATGGCGGGAT 
AAGCATGTGG ACCGCCCTCC TCCAGAAGAG CCCACCATTG GTGACATTTA TAATGGCAAA 
GTTACCAGCA TCATGCAGTT TGGTTGCTTT GTGCAGCTGG AAGGACTAAG GAAGCGGTGG 
GAAGGCCTGG TGCACATCTC TGAGCTCCGG CGGGAGGGTC GTGTGGCCAA TGTAGCTGAT 
GTCGTGAGCA AAGGCCAGAG GGTCAAAGTC AAAGTGCTGT CCTTCACTGG GACCAAGACC 
AGCCTGAGCA TGAAGGATGT GGATCAAGAG ACTGGAGAAG ATCTAAACCC AAATAGACGG 
CGAAATCTTG TCGGGGAGAC CAATGAGGAG ACCTCAATGC GGAATCCTGA TAGACCCACT 
CACTTGTCCC TTGTCAGTGC TCCTGAAGTA GAGGACGACT CACTGGAACG CAAGCGCCTC 
ACCCGAATCT CTGACCCAGA GAAGTGGGAG ATCAAACAGA TGATTGCTGC CAATGTCCTT 
TCCAAAGAAG AATTTCCAGA CTTTGATGAA GAGACTGGCA TTCTCCCTAA GGTGGATGAT 
GAAGAAGATG AGGACCTTGA GATTGAATTG GTTGAGGAAG AGCCTCCATT CCTGAGAGGG 
CACACTAAGC AAAGCATGGA CATGAGCCCC ATTAAAATTG TCAAGAACCC AGACGGCTCC 
CTCTCCCAAG CAGCAATGAT GCAGAGTGCC TTGGCCAAAG AAAGGCGGGA ACTCAAACAG 
GCCCAGCGGG AAGCTGAGAT GGATTCTATT CCCATGGGAC TCAACAAACA CTGGGTTGAC 
CCTCTGCCTG ATGCGGAAGG CAGACAGATT GCTGCCAACA TGAGGGGTAT TGGGATGATG 
CCCAATGATA TTCCTGAGTG GAAGAAGCAT GCCTTTGGGG GCAACAAAGC CTCTTACGGA 
AAAAAGACCC AGATGTCAAT CCTTGAGCAG AGGGAGAGCC TGCCCATCTA CAAACTGAAG 
GAGCAATTGG TCCAGGCCGT CCATGACAAT CAGATCCTGA TTGTCATTGG TGAGACAGGA 
TCTGGAAAGA CAACACAGAT CACCCAGTAC CTGGCGGAGG CAGGCTACAC TTCCAGGGGC 
AAGATTGGGT GTACCCAGCC CAGAAGAGTG GCAGCTATGT CGGTGGCCAA AAGAGTGTCA 
GAGGAGTTTG GTTGTTGCTT AGGCCAAGAG GTGGGCTACA CCATTCGATT TGAGGACTGC 
ACTAGCCCTG AAACAGTCAT CAAGTACATG ACAGATGGGA TGTTGCTTAG AGAGTGCTTG 
ATTGACCCTG ACCTCACTCA GTACGCGATC ATCATGTTGG ACGAGGCACA TGAGAGGACA 
ATTCACACTG ATGTGCTCTT TGGATTGTTG AAAAAGACAG TTCAGAAACG GCAGGACATG 
AAGCTGATTG TCACCTCAGC CACCTTGGAT GCAGTGAAGT TTTCTCAATA CTTCTATGAA 
GCTCCCATTT TCACCATCCC AGGTCGAACA TATCCAGTGG AAATACTGTA CACAAAGGAA 
CCTGAGACAG ATTATCTGGA TGCCAGCCTG ATTACTGTTA TGCAGATTCA TTTAACAGAA 
CCACCAGGTG ATATCCTGGT CTTCCTGACT GGTCAGGAAG AAATTGATAC TGCTTGTGAG 
ATCCTGTATG AAAGAATGAA ATCCCTGGGA CCTGATGTTC CAGAGTTAAT TATCCTCCCA 
GTGTACTCTG CTCTTCCCAG TGAGATGCAG ACCCGAATCT TTGACCCAGC TCCACCAGGC 
AGCAGAAAGG TTGTGATTGC CACCAATATC GCAGAGACAT CGCTGACTAT TGATGGTATC 
TACTATGTGG TGGACCCAGG ATTCGTGAAA CAGAAAGTTT ACAATTCCAA GACAGGGATT 
GACCAGCTCG TGGTGACGCC TATTTCTCAG GCTCAGGCAA AGCAACGAGC TGGCAGAGCT 
GGGAGAACAG GCCCAGGGAA GTGTTACAGG TTGTACACAG AACGTGCCTA CCGAGATGAA 
ATGCTGACCA CCAACGTGCC GGAAATCCAG AGAACCAACT TAGCAAGCAC AGTGCTGTCA 
CTCAAGGCCA TGGGTATCAA TGATCTGCTG TCCTTTGATT TCATGGATGC CCCACCTATG 
GAAACTTTGA TCACAGCCAT GGAGCAGCTG TACACACTGG GGGCCCTGGA TGACGAGGGC 
CTGCTCACTC GCTTGGGCCG CCGGATGGCA GAGTTCCCTC TGGAGCCAAT GCTATGCAAA 
ATGCTCATCA TGTCTGTGCA TCTGGGCTGC AGTGAGGAAA TGCTGACCAT TGTATCCATG 
CTGTCTGTGC AGAACGTCTT CTATAGGCCC AAGGATAAAC AAGCCCTTGC AGATCAGAAG 
AAGGCCAAAT TCCACCAGAC TGAAGGGGAC CACCTCACCC TGCTAGCTGT GTACAACTCC 
TGGAAGAACA ACAAGTTCTC CAACCCATGG TGCTATGAGA ACTTTATCCA GGCTCGTTCC 
CTGCGCCGGG CCCAGGACAT TCGCAAGCAG ATGTTAGGCA TAATGGACAG ACACAAGCTG 
GATGTTGTTT CCTGTGGCAA GTCCACAGTC CGAGTGCAGA AGGCCATCTG CAGTGGGTTC 
TTCCGTAATG CTGCCAAGAA AGACCCGCAG GAGGGTTACC GGACACTGAT CGACCAGCAG 
GTGGTCTATA TCCATCCTTC CAGTGCCCTC TTCAACAGAC AGCCAGAATG GGTGGTGTAC 
CATGAGCTGG TGCTCACCAC CAAGGAATAC ATGCGTGAAG TTACCACCAT CGACCCTCGG 
TGGCTTGTGG AGTTTGCCCC AGCCTTCTTC AAGGTCTCAG ACCCAACTAA GCTAAGCAAA 
CAGAAGAAGC AACAGCGTCT TGAACCCTTG TACAACCGCT ATGAGGAACC CAATGCCTGG 
AGAATATCTC GAGCTTTCCG ACGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_004941.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004932.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1, mRNA.

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CloneID	OHu13153
Clone ID Related Accession (Same CDS sequence)	NM_004941.3 , NM_004941.2
Accession Version	NM_004941.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC310785.1, BC020697.1, D50487.1 and BC044586.1. On Oct 31, 2014 this sequence version replaced NM_004941.1. Transcript Variant:. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047327.1, D50487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGTGGTG TACCATGAGC TGGTGCTCAC CACCAAGGAA 
TACATGCGTG AAGTTACCAC CATCGACCCT CGGTGGCTTG TGGAGTTTGC CCCAGCCTTC 
TTCAAGGTCT CAGACCCAAC TAAGCTAAGC AAACAGAAGA AGCAACAGCG TCTTGAACCC 
TTGTACAACC GCTATGAGGA ACCCAATGCC TGGAGAATAT CTCGAGCTTT CCGACGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004932.1
CDS	122..3784
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	NM_004941.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004941.2

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGGGTGGTG TACCATGAGC TGGTGCTCAC CACCAAGGAA 
TACATGCGTG AAGTTACCAC CATCGACCCT CGGTGGCTTG TGGAGTTTGC CCCAGCCTTC 
TTCAAGGTCT CAGACCCAAC TAAGCTAAGC AAACAGAAGA AGCAACAGCG TCTTGAACCC 
TTGTACAACC GCTATGAGGA ACCCAATGCC TGGAGAATAT CTCGAGCTTT CCGACGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001322221.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309150.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 11, mRNA.

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CloneID	OHu50170
Clone ID Related Accession (Same CDS sequence)	NM_001322221.1
Accession Version	NM_001322221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087650.12 and AC068675.17. On Apr 8, 2016 this sequence version replaced XM_005257113.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.201415.1, SRR1803616.21689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGACAAAG AAGAAGAAGC GGAGTCGAAG CCGAGATCGA 
AACCGAGATC GAGACAGAGA TAGGGAACGA AACCGAGATA GAGACCACAA GCGGAGACAC 
CGATCCCGCT CTCGATCACG TTCCAGGACC CGGGAGAGGA ATAAAGTGAA GTCTAGATAT 
CGGTCCAGGA GCAGGAGTCA GAGTCCCCCC AAAGACCGGA AGGACCGGGA CAAATATGGA 
GAGCGGAATC TGGATAGATG GCGGGATAAG CATGTGGACC GCCCTCCTCC AGAAGAGCCC 
ACCATTGGTG ACATTTATAA TGGCAAAGTT ACCAGCATCA TGCAGTTTGG TTGCTTTGTG 
CAGCTGGAAG GACTAAGGAA GCGGTGGGAA GGCCTGGTGC ACATCTCTGA GCTCCGGCGG 
GAGGGTCGTG TGGCCAATGT AGCTGATGTC GTGAGCAAAG GCCAGAGGGT CAAAGTCAAA 
GTGCTGTCCT TCACTGGGAC CAAGACCAGC CTGAGCATGA AGGATGTGGA TCAAGAGACT 
GGAGAAGATC TAAACCCAAA TAGACGGCGA AATCTTGTCG GGGAGACCAA TGAGGAGACC 
TCAATGCGGA ATCCTGATAG ACCCACTCAC TTGTCCCTTG TCAGTGCTCC TGAAGTAGAG 
GACGACTCAC TGGAACGCAA GCGCCTCACC CGAATCTCTG ACCCAGAGAA GTGGGAGATC 
AAACAGATGA TTGCTGCCAA TGTCCTTTCC AAAGAAGAAT TTCCAGACTT TGATGAAGAG 
ACTGGCATTC TCCCTAAGGT GGATGATGAA GAAGATGAGG ACCTTGAGAT TGAATTGGTT 
GAGGAAGAGC CTCCATTCCT GAGAGGGCAC ACTAAGCAAA GCATGGACAT GAGCCCCATT 
AAAATTGTCA AGAACCCAGA CGGCTCCCTC TCCCAAGCAG CAATGATGCA GAGTGCCTTG 
GCCAAAGAAA GGCGGGAACT CAAACAGGCC CAGCGGGAAG CTGAGATGGA TTCTATTCCC 
ATGGGACTCA ACAAACACTG GGTTGACCCT CTGCCTGATG CGGAAGGCAG ACAGATTGCT 
GCCAACATGA GGGGTATTGG GATGATGCCC AATGATATTC CTGAGTGGAA GAAGCATGCC 
TTTGGGGGCA ACAAAGCCTC TTACGGAAAA AAGACCCAGA TGTCAATCCT TGAGCAGAGG 
GAGAGCCTGC CCATCTACAA ACTGAAGGAG CAATTGGTCC AGGCCGTCCA TGACAATCAG 
ATCCTGATTG TCATTGGTGA GACAGGATCT GGAAAGACAA CACAGATCAC CCAGTACCTG 
GCGGAGGCAG GCTACACTTC CAGGGGCAAG ATTGGGTGTA CCCAGCCCAG AAGAGTGGCA 
GCTATGTCGG TGGCCAAAAG AGTGTCAGAG GAGTTTGGTT GTTGCTTAGG CCAAGAGGTG 
GGCTACACCA TTCGATTTGA GGACTGCACT AGCCCTGAAA CAGTCATCAA GTACATGACA 
GATGGGATGT TGCTTAGAGA GTGCTTGATT GACCCTGACC TCACTCAGTA CGCGATCATC 
ATGTTGGACG AGGCACATGA GAGGACAATT CACACTGATG TGCTCTTTGG ATTGTTGAAA 
AAGACAGTTC AGAAACGGCA GGACATGAAG CTGATTGTCA CCTCAGCCAC CTTGGATGCA 
GTGAAGTTTT CTCAATACTT CTATGAAGCT CCCATTTTCA CCATCCCAGG TCGAACATAT 
CCAGTGGAAA TACTGTACAC AAAGGAACCT GAGACAGATT ATCTGGATGC CAGCCTGATT 
ACTGTTATGC AGATTCATTT AACAGAACCA CCAGGTGATA TCCTGGTCTT CCTGACTGGT 
CAGGAAGAAA TTGATACTGC TTGTGAGATC CTGTATGAAA GAATGAAATC CCTGGGACCT 
GATGTTCCAG AGTTAATTAT CCTCCCAGTG TACTCTGCTC TTCCCAGTGA GATGCAGACC 
CGAATCTTTG ACCCAGCTCC ACCAGGCAGC AGAAAGGTTG TGATTGCCAC CAATATCGCA 
GAGACATCGC TGACTATTGA TGGTATCTAC TATGTGGTGG ACCCAGGATT CGTGAAACAG 
AAAGTTTACA ATTCCAAGAC AGGGATTGAC CAGCTCGTGG TGACGCCTAT TTCTCAGGCT 
CAGGCAAAGC AACGAGCTGG CAGAGCTGGG AGAACAGGCC CAGGGAAGTG TTACAGGTTG 
TACACAGAAC GTGCCTACCG AGATGAAATG CTGACCACCA ACGTGCCGGA AATCCAGAGA 
ACCAACTTAG CAAGCACAGT GCTGTCACTC AAGGCCATGG GTATCAATGA TCTGCTGTCC 
TTTGATTTCA TGGATGCCCC ACCTATGGAA ACTTTGATCA CAGCCATGGA GCAGCTGTAC 
ACACTGGGGG CCCTGGATGA CGAGGGCCTG CTCACTCGCT TGGGCCGCCG GATGGCAGAG 
TTCCCTCTGG AGCCAATGCT ATGCAAAATG CTCATCATGT CTGTGCATCT GGGCTGCAGT 
GAGGAAATGC TGACCATTGT ATCCATGCTG TCTGTGCAGA ACGTCTTCTA TAGGCCCAAG 
GATAAACAAG CCCTTGCAGA TCAGAAGAAG GCCAAATTCC ACCAGACTGA AGGGGACCAC 
CTCACCCTGC TAGCTGTGTA CAACTCCTGG AAGAACAACA AGTTCTCCAA CCCATGGTGC 
TATGAGAACT TTATCCAGGC TCGTTCCCTG CGCCGGGCCC AGGACATTCG CAAGCAGATG 
TTAGGCATAA TGGACAGACA CAAGCTGGAT GTTGTTTCCT GTGGCAAGTC CACAGTCCGA 
GTGCAGAAGG CCATCTGCAG TGGGTTCTTC CGTAATGCTG CCAAGAAAGA CCCGCAGGAG 
GGTTACCGGA CACTGATCGA CCAGCAGGTG GTCTATATCC ATCCTTCCAG TGCCCTCTTC 
AACAGACAGC CAGAATGGGT GGTGTACCAT GAGCTGGTGC TCACCACCAA GGAATACATG 
CGTGAAGTTA CCACCATCGA CCCTCGGTGG CTTGTGGAGT TTGCCCCAGC CTTCTTCAAG 
GTCTCAGACC CAACTAAGCT AAGCAAACAG AAGAAGCAAC AGCGTCTTGA ACCCTTGTAC 
AACCGCTATG AGGAACCCAA TGCCTGGAGA ATATCTCGAG CTTTCCGACG GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309150.1
CDS	174..3830
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	NM_001322221.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322221.1

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGACAAAG AAGAAGAAGC GGAGTCGAAG CCGAGATCGA 
AACCGAGATC GAGACAGAGA TAGGGAACGA AACCGAGATA GAGACCACAA GCGGAGACAC 
CGATCCCGCT CTCGATCACG TTCCAGGACC CGGGAGAGGA ATAAAGTGAA GTCTAGATAT 
CGGTCCAGGA GCAGGAGTCA GAGTCCCCCC AAAGACCGGA AGGACCGGGA CAAATATGGA 
GAGCGGAATC TGGATAGATG GCGGGATAAG CATGTGGACC GCCCTCCTCC AGAAGAGCCC 
ACCATTGGTG ACATTTATAA TGGCAAAGTT ACCAGCATCA TGCAGTTTGG TTGCTTTGTG 
CAGCTGGAAG GACTAAGGAA GCGGTGGGAA GGCCTGGTGC ACATCTCTGA GCTCCGGCGG 
GAGGGTCGTG TGGCCAATGT AGCTGATGTC GTGAGCAAAG GCCAGAGGGT CAAAGTCAAA 
GTGCTGTCCT TCACTGGGAC CAAGACCAGC CTGAGCATGA AGGATGTGGA TCAAGAGACT 
GGAGAAGATC TAAACCCAAA TAGACGGCGA AATCTTGTCG GGGAGACCAA TGAGGAGACC 
TCAATGCGGA ATCCTGATAG ACCCACTCAC TTGTCCCTTG TCAGTGCTCC TGAAGTAGAG 
GACGACTCAC TGGAACGCAA GCGCCTCACC CGAATCTCTG ACCCAGAGAA GTGGGAGATC 
AAACAGATGA TTGCTGCCAA TGTCCTTTCC AAAGAAGAAT TTCCAGACTT TGATGAAGAG 
ACTGGCATTC TCCCTAAGGT GGATGATGAA GAAGATGAGG ACCTTGAGAT TGAATTGGTT 
GAGGAAGAGC CTCCATTCCT GAGAGGGCAC ACTAAGCAAA GCATGGACAT GAGCCCCATT 
AAAATTGTCA AGAACCCAGA CGGCTCCCTC TCCCAAGCAG CAATGATGCA GAGTGCCTTG 
GCCAAAGAAA GGCGGGAACT CAAACAGGCC CAGCGGGAAG CTGAGATGGA TTCTATTCCC 
ATGGGACTCA ACAAACACTG GGTTGACCCT CTGCCTGATG CGGAAGGCAG ACAGATTGCT 
GCCAACATGA GGGGTATTGG GATGATGCCC AATGATATTC CTGAGTGGAA GAAGCATGCC 
TTTGGGGGCA ACAAAGCCTC TTACGGAAAA AAGACCCAGA TGTCAATCCT TGAGCAGAGG 
GAGAGCCTGC CCATCTACAA ACTGAAGGAG CAATTGGTCC AGGCCGTCCA TGACAATCAG 
ATCCTGATTG TCATTGGTGA GACAGGATCT GGAAAGACAA CACAGATCAC CCAGTACCTG 
GCGGAGGCAG GCTACACTTC CAGGGGCAAG ATTGGGTGTA CCCAGCCCAG AAGAGTGGCA 
GCTATGTCGG TGGCCAAAAG AGTGTCAGAG GAGTTTGGTT GTTGCTTAGG CCAAGAGGTG 
GGCTACACCA TTCGATTTGA GGACTGCACT AGCCCTGAAA CAGTCATCAA GTACATGACA 
GATGGGATGT TGCTTAGAGA GTGCTTGATT GACCCTGACC TCACTCAGTA CGCGATCATC 
ATGTTGGACG AGGCACATGA GAGGACAATT CACACTGATG TGCTCTTTGG ATTGTTGAAA 
AAGACAGTTC AGAAACGGCA GGACATGAAG CTGATTGTCA CCTCAGCCAC CTTGGATGCA 
GTGAAGTTTT CTCAATACTT CTATGAAGCT CCCATTTTCA CCATCCCAGG TCGAACATAT 
CCAGTGGAAA TACTGTACAC AAAGGAACCT GAGACAGATT ATCTGGATGC CAGCCTGATT 
ACTGTTATGC AGATTCATTT AACAGAACCA CCAGGTGATA TCCTGGTCTT CCTGACTGGT 
CAGGAAGAAA TTGATACTGC TTGTGAGATC CTGTATGAAA GAATGAAATC CCTGGGACCT 
GATGTTCCAG AGTTAATTAT CCTCCCAGTG TACTCTGCTC TTCCCAGTGA GATGCAGACC 
CGAATCTTTG ACCCAGCTCC ACCAGGCAGC AGAAAGGTTG TGATTGCCAC CAATATCGCA 
GAGACATCGC TGACTATTGA TGGTATCTAC TATGTGGTGG ACCCAGGATT CGTGAAACAG 
AAAGTTTACA ATTCCAAGAC AGGGATTGAC CAGCTCGTGG TGACGCCTAT TTCTCAGGCT 
CAGGCAAAGC AACGAGCTGG CAGAGCTGGG AGAACAGGCC CAGGGAAGTG TTACAGGTTG 
TACACAGAAC GTGCCTACCG AGATGAAATG CTGACCACCA ACGTGCCGGA AATCCAGAGA 
ACCAACTTAG CAAGCACAGT GCTGTCACTC AAGGCCATGG GTATCAATGA TCTGCTGTCC 
TTTGATTTCA TGGATGCCCC ACCTATGGAA ACTTTGATCA CAGCCATGGA GCAGCTGTAC 
ACACTGGGGG CCCTGGATGA CGAGGGCCTG CTCACTCGCT TGGGCCGCCG GATGGCAGAG 
TTCCCTCTGG AGCCAATGCT ATGCAAAATG CTCATCATGT CTGTGCATCT GGGCTGCAGT 
GAGGAAATGC TGACCATTGT ATCCATGCTG TCTGTGCAGA ACGTCTTCTA TAGGCCCAAG 
GATAAACAAG CCCTTGCAGA TCAGAAGAAG GCCAAATTCC ACCAGACTGA AGGGGACCAC 
CTCACCCTGC TAGCTGTGTA CAACTCCTGG AAGAACAACA AGTTCTCCAA CCCATGGTGC 
TATGAGAACT TTATCCAGGC TCGTTCCCTG CGCCGGGCCC AGGACATTCG CAAGCAGATG 
TTAGGCATAA TGGACAGACA CAAGCTGGAT GTTGTTTCCT GTGGCAAGTC CACAGTCCGA 
GTGCAGAAGG CCATCTGCAG TGGGTTCTTC CGTAATGCTG CCAAGAAAGA CCCGCAGGAG 
GGTTACCGGA CACTGATCGA CCAGCAGGTG GTCTATATCC ATCCTTCCAG TGCCCTCTTC 
AACAGACAGC CAGAATGGGT GGTGTACCAT GAGCTGGTGC TCACCACCAA GGAATACATG 
CGTGAAGTTA CCACCATCGA CCCTCGGTGG CTTGTGGAGT TTGCCCCAGC CTTCTTCAAG 
GTCTCAGACC CAACTAAGCT AAGCAAACAG AAGAAGCAAC AGCGTCTTGA ACCCTTGTAC 
AACCGCTATG AGGAACCCAA TGCCTGGAGA ATATCTCGAG CTTTCCGACG GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001302623.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289552.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu50171
Clone ID Related Accession (Same CDS sequence)	NM_001302623.1 , NM_001302623.2
Accession Version	NM_001302623.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC310785.1, BC020697.1, D50487.1, AK303319.1, BE890949.1 and BC038223.1. On Oct 31, 2014 this sequence version replaced XM_005257114.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303319.1, SRR1803611.258110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGCCCAAAG CACTTCCTCC CTGTGGTTGC TGTAGCTGTC 
TCATTGGAGC AGTGCCTCTC CAAGTTTGAG GATTTGAACA AAGAACTGGG ACTGGTGACT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289552.1
CDS	122..3667
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECPKHFLPVVAVAVSLEQCLSKFEDLNKELGLVTC

Target ORF information:

RefSeq Version	NM_001302623.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302623.1

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGCCCAAAG CACTTCCTCC CTGTGGTTGC TGTAGCTGTC 
TCATTGGAGC AGTGCCTCTC CAAGTTTGAG GATTTGAACA AAGAACTGGG ACTGGTGACT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX8 ( NM_001302623.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289552.1 Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 2, mRNA.

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CloneID	OHu50171
Clone ID Related Accession (Same CDS sequence)	NM_001302623.1 , NM_001302623.2
Accession Version	NM_001302623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DHX8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D50487.1, AK303319.1, BE890949.1, BC038223.1 and AC068675.17. On May 31, 2019 this sequence version replaced NM_001302623.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303319.1, SRR1803611.258110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGCCCAAAG CACTTCCTCC CTGTGGTTGC TGTAGCTGTC 
TCATTGGAGC AGTGCCTCTC CAAGTTTGAG GATTTGAACA AAGAACTGGG ACTGGTGACT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289552.1
CDS	64..3609
Translation	MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECPKHFLPVVAVAVSLEQCLSKFEDLNKELGLVTC

Target ORF information:

RefSeq Version	NM_001302623.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 8 (DHX8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302623.2

ORF Insert Sequence:

ATGGCTGTGG CTGTAGCCAT GGCGGGAGCC TTAATCGGGT CGGAGCCAGG CCCCGCGGAA 
GAACTTGCCA AACTCGAGTA CCTGTCTTTG GTGTCAAAGG TTTGCACTGA GCTGGACAAT 
CACTTGGGGA TCAACGACAA GGACCTTGCT GAATTTGTGA TCAGTCTTGC TGAGAAAAAT 
ACCACCTTTG ATACTTTTAA GGCTTCTCTC GTCAAAAATG GTGCAGAATT TACGGATTCT 
CTTATTAGTA ACTTGCTGCG TCTCATACAA ACCATGCGGC CTCCAGCGAA GCCTTCCACT 
AGCAAAGATC CAGTTGTTAA ACCTAAAACA GAAAAAGAAA AGCTGAAGGA ACTCTTCCCA 
GTCCTTTGCC AACCGGACAA CCCTTCTGTT CGGACCATGT TGGATGAAGA TGATGTGAAA 
GTTGCTGTGG ATGTCCTGAA AGAACTGGAA GCTTTAATGC CCAGCGCAGC AGGCCAGGAG 
AAGCAAAGAG ATGCTGAACA CCGGGACAGG ACAAAGAAGA AGAAGCGGAG TCGAAGCCGA 
GATCGAAACC GAGATCGAGA CAGAGATAGG GAACGAAACC GAGATAGAGA CCACAAGCGG 
AGACACCGAT CCCGCTCTCG ATCACGTTCC AGGACCCGGG AGAGGAATAA AGTGAAGTCT 
AGATATCGGT CCAGGAGCAG GAGTCAGAGT CCCCCCAAAG ACCGGAAGGA CCGGGACAAA 
TATGGAGAGC GGAATCTGGA TAGATGGCGG GATAAGCATG TGGACCGCCC TCCTCCAGAA 
GAGCCCACCA TTGGTGACAT TTATAATGGC AAAGTTACCA GCATCATGCA GTTTGGTTGC 
TTTGTGCAGC TGGAAGGACT AAGGAAGCGG TGGGAAGGCC TGGTGCACAT CTCTGAGCTC 
CGGCGGGAGG GTCGTGTGGC CAATGTAGCT GATGTCGTGA GCAAAGGCCA GAGGGTCAAA 
GTCAAAGTGC TGTCCTTCAC TGGGACCAAG ACCAGCCTGA GCATGAAGGA TGTGGATCAA 
GAGACTGGAG AAGATCTAAA CCCAAATAGA CGGCGAAATC TTGTCGGGGA GACCAATGAG 
GAGACCTCAA TGCGGAATCC TGATAGACCC ACTCACTTGT CCCTTGTCAG TGCTCCTGAA 
GTAGAGGACG ACTCACTGGA ACGCAAGCGC CTCACCCGAA TCTCTGACCC AGAGAAGTGG 
GAGATCAAAC AGATGATTGC TGCCAATGTC CTTTCCAAAG AAGAATTTCC AGACTTTGAT 
GAAGAGACTG GCATTCTCCC TAAGGTGGAT GATGAAGAAG ATGAGGACCT TGAGATTGAA 
TTGGTTGAGG AAGAGCCTCC ATTCCTGAGA GGGCACACTA AGCAAAGCAT GGACATGAGC 
CCCATTAAAA TTGTCAAGAA CCCAGACGGC TCCCTCTCCC AAGCAGCAAT GATGCAGAGT 
GCCTTGGCCA AAGAAAGGCG GGAACTCAAA CAGGCCCAGC GGGAAGCTGA GATGGATTCT 
ATTCCCATGG GACTCAACAA ACACTGGGTT GACCCTCTGC CTGATGCGGA AGGCAGACAG 
ATTGCTGCCA ACATGAGGGG TATTGGGATG ATGCCCAATG ATATTCCTGA GTGGAAGAAG 
CATGCCTTTG GGGGCAACAA AGCCTCTTAC GGAAAAAAGA CCCAGATGTC AATCCTTGAG 
CAGAGGGAGA GCCTGCCCAT CTACAAACTG AAGGAGCAAT TGGTCCAGGC CGTCCATGAC 
AATCAGATCC TGATTGTCAT TGGTGAGACA GGATCTGGAA AGACAACACA GATCACCCAG 
TACCTGGCGG AGGCAGGCTA CACTTCCAGG GGCAAGATTG GGTGTACCCA GCCCAGAAGA 
GTGGCAGCTA TGTCGGTGGC CAAAAGAGTG TCAGAGGAGT TTGGTTGTTG CTTAGGCCAA 
GAGGTGGGCT ACACCATTCG ATTTGAGGAC TGCACTAGCC CTGAAACAGT CATCAAGTAC 
ATGACAGATG GGATGTTGCT TAGAGAGTGC TTGATTGACC CTGACCTCAC TCAGTACGCG 
ATCATCATGT TGGACGAGGC ACATGAGAGG ACAATTCACA CTGATGTGCT CTTTGGATTG 
TTGAAAAAGA CAGTTCAGAA ACGGCAGGAC ATGAAGCTGA TTGTCACCTC AGCCACCTTG 
GATGCAGTGA AGTTTTCTCA ATACTTCTAT GAAGCTCCCA TTTTCACCAT CCCAGGTCGA 
ACATATCCAG TGGAAATACT GTACACAAAG GAACCTGAGA CAGATTATCT GGATGCCAGC 
CTGATTACTG TTATGCAGAT TCATTTAACA GAACCACCAG GTGATATCCT GGTCTTCCTG 
ACTGGTCAGG AAGAAATTGA TACTGCTTGT GAGATCCTGT ATGAAAGAAT GAAATCCCTG 
GGACCTGATG TTCCAGAGTT AATTATCCTC CCAGTGTACT CTGCTCTTCC CAGTGAGATG 
CAGACCCGAA TCTTTGACCC AGCTCCACCA GGCAGCAGAA AGGTTGTGAT TGCCACCAAT 
ATCGCAGAGA CATCGCTGAC TATTGATGGT ATCTACTATG TGGTGGACCC AGGATTCGTG 
AAACAGAAAG TTTACAATTC CAAGACAGGG ATTGACCAGC TCGTGGTGAC GCCTATTTCT 
CAGGCTCAGG CAAAGCAACG AGCTGGCAGA GCTGGGAGAA CAGGCCCAGG GAAGTGTTAC 
AGGTTGTACA CAGAACGTGC CTACCGAGAT GAAATGCTGA CCACCAACGT GCCGGAAATC 
CAGAGAACCA ACTTAGCAAG CACAGTGCTG TCACTCAAGG CCATGGGTAT CAATGATCTG 
CTGTCCTTTG ATTTCATGGA TGCCCCACCT ATGGAAACTT TGATCACAGC CATGGAGCAG 
CTGTACACAC TGGGGGCCCT GGATGACGAG GGCCTGCTCA CTCGCTTGGG CCGCCGGATG 
GCAGAGTTCC CTCTGGAGCC AATGCTATGC AAAATGCTCA TCATGTCTGT GCATCTGGGC 
TGCAGTGAGG AAATGCTGAC CATTGTATCC ATGCTGTCTG TGCAGAACGT CTTCTATAGG 
CCCAAGGATA AACAAGCCCT TGCAGATCAG AAGAAGGCCA AATTCCACCA GACTGAAGGG 
GACCACCTCA CCCTGCTAGC TGTGTACAAC TCCTGGAAGA ACAACAAGTT CTCCAACCCA 
TGGTGCTATG AGAACTTTAT CCAGGCTCGT TCCCTGCGCC GGGCCCAGGA CATTCGCAAG 
CAGATGTTAG GCATAATGGA CAGACACAAG CTGGATGTTG TTTCCTGTGG CAAGTCCACA 
GTCCGAGTGC AGAAGGCCAT CTGCAGTGGG TTCTTCCGTA ATGCTGCCAA GAAAGACCCG 
CAGGAGGGTT ACCGGACACT GATCGACCAG CAGGTGGTCT ATATCCATCC TTCCAGTGCC 
CTCTTCAACA GACAGCCAGA ATGCCCAAAG CACTTCCTCC CTGTGGTTGC TGTAGCTGTC 
TCATTGGAGC AGTGCCTCTC CAAGTTTGAG GATTTGAACA AAGAACTGGG ACTGGTGACT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DHX8
Entrez Gene ID	1659
Full Name	DEAH-box helicase 8
Synonyms	DDX8,Dhr2,HRH1,PRP22,PRPF22
General protein information	Preferred Names DEAH-box helicase 8 Names ATP-dependent RNA helicase DHX8 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase) DEAH (Asp-Glu-Ala-His) box polypeptide 8 DEAH box protein 8 RNA helicase HRH1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.31
Summary	This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].
Disorder MIM:	600396

Pan troglodytes (chimpanzee)	DHX8	XP_001154202.1
Canis lupus familiaris (dog)	DHX8	XP_537627.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007212	XP_308573.4
Oryza sativa (rice)	Os06g0343100	NP_001057574.1
Homo sapiens (human)	DHX8	NP_004932.1
Danio rerio (zebrafish)	dhx8	XP_002663993.1
Saccharomyces cerevisiae (baker's yeast)	PRP22	NP_010929.3
Schizosaccharomyces pombe (fission yeast)	prp22	NP_593253.1
Xenopus tropicalis (tropical clawed frog)	LOC733563	XP_002932570.2
Bos taurus (cattle)	DHX8	NP_001193020.1
Mus musculus (house mouse)	Dhx8	NP_659080.2
Magnaporthe oryzae (rice blast fungus)	MGG_08807	XP_003719164.1
	DHX8	XP_002800602.1
Gallus gallus (chicken)	DHX8	XP_418105.3
Kluyveromyces lactis	KLLA0B11638g	XP_452048.1
Neurospora crassa	NCU06318	XP_962580.1
Rattus norvegicus (Norway rat)	Dhx8	NP_001041309.1
Caenorhabditis elegans (roundworm)	mog-5	NP_495019.1
Eremothecium gossypii	AGOS_AAR020W	NP_982561.2
Arabidopsis thaliana (thale cress)	AT3G26560	NP_189288.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DHX8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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