LOC108645188 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108645188
Entrez Gene ID	108645188
Full Name	putative cell agglutination protein 1742.01
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018090054.1	XP_017945543.1	putative cell agglutination protein 1742.01 isoform X2
XM_018090056.1	XP_017945545.1	putative cell agglutination protein 1742.01 isoform X4
XM_031905243.1	XP_031761103.1	mucin-5AC-like isoform X10
XM_031905248.1	XP_031761108.1	mucin-5AC-like isoform X15
XM_031905251.1	XP_031761111.1	mucin-5AC-like isoform X18
XM_031905253.1	XP_031761113.1	mucin-5AC-like isoform X20
XM_031905258.1	XP_031761118.1	mucin-5AC-like isoform X25
XM_031905260.1	XP_031761120.1	mucin-5AC-like isoform X27
XM_031905266.1	XP_031761126.1	mucin-5AC-like isoform X33
XM_031905269.1	XP_031761129.1	mucin-5AC-like isoform X36
XM_031905271.1	XP_031761131.1	mucin-5AC-like isoform X38
XM_031905272.1	XP_031761132.1	serine-rich adhesin for platelets-like isoform X39
XM_031905275.1	XP_031761135.1	serine-rich adhesin for platelets-like isoform X42
XM_031905280.1	XP_031761140.1	putative GPI-anchored protein pfl2 isoform X47
XM_031905282.1	XP_031761142.1	serine-rich adhesin for platelets-like isoform X49
XM_018090055.1	XP_017945544.1	period circadian protein-like isoform X3
XM_031905245.1	XP_031761105.1	mucin-5AC-like isoform X12
XM_031905249.1	XP_031761109.1	serine-rich adhesin for platelets-like isoform X16
XM_031905256.1	XP_031761116.1	serine-rich adhesin for platelets-like isoform X23
XM_031905261.1	XP_031761121.1	mucin-5AC-like isoform X28
XM_031905262.1	XP_031761122.1	serine-rich adhesin for platelets-like isoform X29
XM_031905264.1	XP_031761124.1	mucin-5AC-like isoform X31
XM_031905267.1	XP_031761127.1	mucin-5AC-like isoform X34
XM_031905273.1	XP_031761133.1	serine-rich adhesin for platelets-like isoform X40
XM_031905274.1	XP_031761134.1	serine-rich adhesin for platelets-like isoform X41
XM_031905279.1	XP_031761139.1	putative GPI-anchored protein pfl2 isoform X46
XM_031905234.1	XP_031761094.1	mucin-5AC-like isoform X2
XM_031905236.1	XP_031761096.1	mucin-5AC-like isoform X3
XM_031905237.1	XP_031761097.1	mucin-5AC-like isoform X4
XM_031905240.1	XP_031761100.1	mucin-5AC-like isoform X7
XM_031905246.1	XP_031761106.1	mucin-5AC-like isoform X13
XM_031905250.1	XP_031761110.1	mucin-5AC-like isoform X17
XM_031905254.1	XP_031761114.1	mucin-5AC-like isoform X21
XM_031905257.1	XP_031761117.1	uncharacterized threonine-rich GPI-anchored glycoprotein PJ4664.02-like isoform X24
XM_031905259.1	XP_031761119.1	mucin-5AC-like isoform X26
XM_031905268.1	XP_031761128.1	mucin-5AC-like isoform X35
XM_031905270.1	XP_031761130.1	mucin-5AC-like isoform X37
XM_031905277.1	XP_031761137.1	putative GPI-anchored protein pfl2 isoform X44
XM_018090053.1	XP_017945542.1	cell wall protein AWA1-like isoform X1
XM_018090057.1	XP_017945546.1	period circadian protein-like isoform X5
XM_031905239.1	XP_031761099.1	serine-rich adhesin for platelets-like isoform X6
XM_031905241.1	XP_031761101.1	putative GPI-anchored protein pfl2 isoform X8
XM_031905242.1	XP_031761102.1	serine-rich adhesin for platelets-like isoform X9
XM_031905244.1	XP_031761104.1	mucin-5AC-like isoform X11
XM_031905247.1	XP_031761107.1	mucin-5AC-like isoform X14
XM_031905252.1	XP_031761112.1	mucin-5AC-like isoform X19
XM_031905283.1	XP_031761143.1	serine-rich adhesin for platelets-like isoform X50
XM_031905233.1	XP_031761093.1	mucin-5AC-like isoform X1
XM_031905238.1	XP_031761098.1	mucin-5AC-like isoform X5
XM_031905255.1	XP_031761115.1	mucin-5AC-like isoform X22
XM_031905263.1	XP_031761123.1	serine-rich adhesin for platelets-like isoform X30
XM_031905265.1	XP_031761125.1	mucin-5AC-like isoform X32
XM_031905276.1	XP_031761136.1	serine-rich adhesin for platelets-like isoform X43
XM_031905278.1	XP_031761138.1	putative GPI-anchored protein pfl2 isoform X45
XM_031905281.1	XP_031761141.1	serine-rich adhesin for platelets-like isoform X48

Pathways from BioSystems
Gene Ontology

The following LOC108645188 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108645188 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa41132	XM_018090053.1 Latest version!	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa41133	XM_018090054.1 Latest version!	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa41134	XM_018090055.1 Latest version!	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa41135	XM_018090056.1 Latest version!	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa41136	XM_018090057.1 Latest version!	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OXa61567	XM_031905233.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61568	XM_031905234.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61569	XM_031905236.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61570	XM_031905237.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61571	XM_031905238.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61572	XM_031905239.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61573	XM_031905240.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61574	XM_031905241.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61575	XM_031905242.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61576	XM_031905243.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61577	XM_031905244.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61577	XM_031905245.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61578	XM_031905246.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61579	XM_031905247.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61580	XM_031905248.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61581	XM_031905249.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61582	XM_031905250.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61583	XM_031905251.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61584	XM_031905252.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61585	XM_031905253.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61586	XM_031905254.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61587	XM_031905255.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61588	XM_031905256.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61589	XM_031905257.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61590	XM_031905258.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61591	XM_031905259.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61592	XM_031905260.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61593	XM_031905261.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61594	XM_031905262.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61595	XM_031905263.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61596	XM_031905264.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61597	XM_031905265.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61598	XM_031905266.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61599	XM_031905267.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61600	XM_031905268.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61601	XM_031905269.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61602	XM_031905270.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61603	XM_031905271.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61604	XM_031905272.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61605	XM_031905273.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61606	XM_031905274.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61607	XM_031905275.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61608	XM_031905276.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61609	XM_031905277.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61610	XM_031905278.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61611	XM_031905279.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61612	XM_031905280.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61613	XM_031905281.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X48, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61614	XM_031905282.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X49, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61615	XM_031905283.1 Latest version!	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X50, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108645188 ( XM_018090053.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945542.1 Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa41132
Clone ID Related Accession (Same CDS sequence)	XM_018090053.1
Accession Version	XM_018090053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cell wall protein AWA1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GCACTCACTG CTCCAGTGAC AAATGGTACG TCAGCAGCTG GGACATCTGG CACTGTGACA 
AATGGTACGT CAGCAGCTGG GACATCTGGC ACTGTGACAA ATGGTACGTC AGCAGCTGGG 
ACATCTGGCA CTGCACTCAC TGCTCCAGTA ACAAATGGTA CATCAGCAGC AGGGACATCC 
GGCACTGTGA CAAGCGGTAC AGCAACAGCA GGGACATCCG GCACTGGCTC AGCAGTCACT 
ACAGCAACTC AAGCAGCAAC TGGGACATCC AGCACTGCTT TCTCTACTCT AAACACAAGT 
AACACGTCAT CAGCTGCGGC ATCTGGCACT GCTCTCTCTA CTCTCAAGGC CAGTAATACA 
TCGACAGCTG TGACATCCGG CTCAGCACCC AGTATAGTCA TGGAGAGAGG AGTGACAACC 
TGGAATATTC AAGGAGCATT TACTACCTGC AAAATATTTG TGTATATCTC TTTATATCTA 
TGTTTTTCTT TTTTTAACTT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945542.1
CDS	66..752
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSGTALTAPVTSATSTAGTSGTALTAPVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTAFSTLNTSNTSSAAASGTALSTLKASNTSTAVTSGSAPSIVMERGVTTWNIQGAFTTCKIFVYISLYLCFSFFNFM

Target ORF information:

RefSeq Version	XM_018090053.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090053.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GCACTCACTG CTCCAGTGAC AAATGGTACG TCAGCAGCTG GGACATCTGG CACTGTGACA 
AATGGTACGT CAGCAGCTGG GACATCTGGC ACTGTGACAA ATGGTACGTC AGCAGCTGGG 
ACATCTGGCA CTGCACTCAC TGCTCCAGTA ACAAATGGTA CATCAGCAGC AGGGACATCC 
GGCACTGTGA CAAGCGGTAC AGCAACAGCA GGGACATCCG GCACTGGCTC AGCAGTCACT 
ACAGCAACTC AAGCAGCAAC TGGGACATCC AGCACTGCTT TCTCTACTCT AAACACAAGT 
AACACGTCAT CAGCTGCGGC ATCTGGCACT GCTCTCTCTA CTCTCAAGGC CAGTAATACA 
TCGACAGCTG TGACATCCGG CTCAGCACCC AGTATAGTCA TGGAGAGAGG AGTGACAACC 
TGGAATATTC AAGGAGCATT TACTACCTGC AAAATATTTG TGTATATCTC TTTATATCTA 
TGTTTTTCTT TTTTTAACTT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_018090054.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945543.1 Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa41133
Clone ID Related Accession (Same CDS sequence)	XM_018090054.1
Accession Version	XM_018090054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative cell agglutination protein 1742.01 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GTGACAAATG GTACGTCAGC AGCTGGGACA TCTGGCACTG TGACAAATGG TACGTCAGCA 
GCTGGGACAT CTGGCACTGT GACAAATGGT ACGTCAGCAG CTGGGACATC TGGCACTGCA 
CTCACTGCTC CAGTAACAAA TGGTACATCA GCAGCAGGGA CATCCGGCAC TGTGACAAGC 
GGTACAGCAA CAGCAGGGAC ATCCGGCACT GGCTCAGCAG TCACTACAGC AACTCAAGCA 
GCAACTGGGA CATCCAGCAC TGCTTTCTCT ACTCTAAACA CAAGTAACAC GTCATCAGCT 
GCGGCATCTG GCACTGCTCT CTCTACTCTC AAGGCCAGTA ATACATCGAC AGCTGTGACA 
TCCGGCTCAG CACCCAGTAT AGTCATGGAG AGAGGAGTGA CAACCTGGAA TATTCAAGGA 
GCATTTACTA CCTGCAAAAT ATTTGTGTAT ATCTCTTTAT ATCTATGTTT TTCTTTTTTT 
AACTTCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945543.1
CDS	73..744
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSGTALTAPVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTAFSTLNTSNTSSAAASGTALSTLKASNTSTAVTSGSAPSIVMERGVTTWNIQGAFTTCKIFVYISLYLCFSFFNFM

Target ORF information:

RefSeq Version	XM_018090054.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090054.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GTGACAAATG GTACGTCAGC AGCTGGGACA TCTGGCACTG TGACAAATGG TACGTCAGCA 
GCTGGGACAT CTGGCACTGT GACAAATGGT ACGTCAGCAG CTGGGACATC TGGCACTGCA 
CTCACTGCTC CAGTAACAAA TGGTACATCA GCAGCAGGGA CATCCGGCAC TGTGACAAGC 
GGTACAGCAA CAGCAGGGAC ATCCGGCACT GGCTCAGCAG TCACTACAGC AACTCAAGCA 
GCAACTGGGA CATCCAGCAC TGCTTTCTCT ACTCTAAACA CAAGTAACAC GTCATCAGCT 
GCGGCATCTG GCACTGCTCT CTCTACTCTC AAGGCCAGTA ATACATCGAC AGCTGTGACA 
TCCGGCTCAG CACCCAGTAT AGTCATGGAG AGAGGAGTGA CAACCTGGAA TATTCAAGGA 
GCATTTACTA CCTGCAAAAT ATTTGTGTAT ATCTCTTTAT ATCTATGTTT TTCTTTTTTT 
AACTTCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_018090055.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945544.1 Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa41134
Clone ID Related Accession (Same CDS sequence)	XM_018090055.1
Accession Version	XM_018090055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	period circadian protein-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GCACTCACTG CTCCAGTGAC AAATGGTACG TCAGCAGCTG GGACATCTGG CACTGTGACA 
AATGGTACGT CAGCAGCTGG GACATCTGGC ACTGTGACAA ATGGTACGTC AGCAGCTGGG 
ACATCTGGCA CTGCACTCAC TGCTCCAGTA ACAAATGGTA CATCAGCAGC AGGGACATCC 
GGCACTGGCT CAGCAGTCAC TACAGCAACT CAAGCAGCAA CTGGGACATC CAGCACTGCT 
TTCTCTACTC TAAACACAAG TAACACGTCA TCAGCTGCGG CATCTGGCAC TGCTCTCTCT 
ACTCTCAAGG CCAGTAATAC ATCGACAGCT GTGACATCCG GCTCAGCACC CAGTATAGTC 
ATGGAGAGAG GAGTGACAAC CTGGAATATT CAAGGAGCAT TTACTACCTG CAAAATATTT 
GTGTATATCT CTTTATATCT ATGTTTTTCT TTTTTTAACT TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945544.1
CDS	69..716
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSGTALTAPVTSATSTAGTSGTALTAPVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTGSAVTTATQAATGTSSTAFSTLNTSNTSSAAASGTALSTLKASNTSTAVTSGSAPSIVMERGVTTWNIQGAFTTCKIFVYISLYLCFSFFNFM

Target ORF information:

RefSeq Version	XM_018090055.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090055.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GCACTCACTG CTCCAGTGAC AAATGGTACG TCAGCAGCTG GGACATCTGG CACTGTGACA 
AATGGTACGT CAGCAGCTGG GACATCTGGC ACTGTGACAA ATGGTACGTC AGCAGCTGGG 
ACATCTGGCA CTGCACTCAC TGCTCCAGTA ACAAATGGTA CATCAGCAGC AGGGACATCC 
GGCACTGGCT CAGCAGTCAC TACAGCAACT CAAGCAGCAA CTGGGACATC CAGCACTGCT 
TTCTCTACTC TAAACACAAG TAACACGTCA TCAGCTGCGG CATCTGGCAC TGCTCTCTCT 
ACTCTCAAGG CCAGTAATAC ATCGACAGCT GTGACATCCG GCTCAGCACC CAGTATAGTC 
ATGGAGAGAG GAGTGACAAC CTGGAATATT CAAGGAGCAT TTACTACCTG CAAAATATTT 
GTGTATATCT CTTTATATCT ATGTTTTTCT TTTTTTAACT TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_018090056.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945545.1 Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa41135
Clone ID Related Accession (Same CDS sequence)	XM_018090056.1
Accession Version	XM_018090056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative cell agglutination protein 1742.01 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GTGACAAATG GTACGTCAGC AGCTGGGACA TCTGGCACTG TGACAAATGG TACGTCAGCA 
GCTGGGACAT CTGGCACTGC ACTCACTGCT CCAGTAACAA ATGGTACATC AGCAGCAGGG 
ACATCCGGCA CTGTGACAAG CGGTACAGCA ACAGCAGGGA CATCCGGCAC TGGCTCAGCA 
GTCACTACAG CAACTCAAGC AGCAACTGGG ACATCCAGCA CTGCTTTCTC TACTCTAAAC 
ACAAGTAACA CGTCATCAGC TGCGGCATCT GGCACTGCTC TCTCTACTCT CAAGGCCAGT 
AATACATCGA CAGCTGTGAC ATCCGGCTCA GCACCCAGTA TAGTCATGGA GAGAGGAGTG 
ACAACCTGGA ATATTCAAGG AGCATTTACT ACCTGCAAAA TATTTGTGTA TATCTCTTTA 
TATCTATGTT TTTCTTTTTT TAACTTCATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945545.1
CDS	72..704
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSGTALTAPVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTAFSTLNTSNTSSAAASGTALSTLKASNTSTAVTSGSAPSIVMERGVTTWNIQGAFTTCKIFVYISLYLCFSFFNFM

Target ORF information:

RefSeq Version	XM_018090056.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090056.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GTGACAAATG GTACGTCAGC AGCTGGGACA TCTGGCACTG TGACAAATGG TACGTCAGCA 
GCTGGGACAT CTGGCACTGC ACTCACTGCT CCAGTAACAA ATGGTACATC AGCAGCAGGG 
ACATCCGGCA CTGTGACAAG CGGTACAGCA ACAGCAGGGA CATCCGGCAC TGGCTCAGCA 
GTCACTACAG CAACTCAAGC AGCAACTGGG ACATCCAGCA CTGCTTTCTC TACTCTAAAC 
ACAAGTAACA CGTCATCAGC TGCGGCATCT GGCACTGCTC TCTCTACTCT CAAGGCCAGT 
AATACATCGA CAGCTGTGAC ATCCGGCTCA GCACCCAGTA TAGTCATGGA GAGAGGAGTG 
ACAACCTGGA ATATTCAAGG AGCATTTACT ACCTGCAAAA TATTTGTGTA TATCTCTTTA 
TATCTATGTT TTTCTTTTTT TAACTTCATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_018090057.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945546.1 Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa41136
Clone ID Related Accession (Same CDS sequence)	XM_018090057.1
Accession Version	XM_018090057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	period circadian protein-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GCACTCACTG CTCCAGTAAC AAATGGTACA TCAGCAGCAG GGACATCCGG CACTGTGACA 
AGCGGTACAG CAACAGCAGG GACATCCGGC ACTGGCTCAG CAGTCACTAC AGCAACTCAA 
GCAGCAACTG GGACATCCAG CACTGCTTTC TCTACTCTAA ACACAAGTAA CACGTCATCA 
GCTGCGGCAT CTGGCACTGC TCTCTCTACT CTCAAGGCCA GTAATACATC GACAGCTGTG 
ACATCCGGCT CAGCACCCAG TATAGTCATG GAGAGAGGAG TGACAACCTG GAATATTCAA 
GGAGCATTTA CTACCTGCAA AATATTTGTG TATATCTCTT TATATCTATG TTTTTCTTTT 
TTTAACTTCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945546.1
CDS	64..618
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSGTALTAPVTSATSTAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTAFSTLNTSNTSSAAASGTALSTLKASNTSTAVTSGSAPSIVMERGVTTWNIQGAFTTCKIFVYISLYLCFSFFNFM

Target ORF information:

RefSeq Version	XM_018090057.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative cell agglutination protein 1742.01 (LOC108645188), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090057.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
GGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCGGCACC 
GCACTCACTG CTCCAGTAAC AAATGGTACA TCAGCAGCAG GGACATCCGG CACTGTGACA 
AGCGGTACAG CAACAGCAGG GACATCCGGC ACTGGCTCAG CAGTCACTAC AGCAACTCAA 
GCAGCAACTG GGACATCCAG CACTGCTTTC TCTACTCTAA ACACAAGTAA CACGTCATCA 
GCTGCGGCAT CTGGCACTGC TCTCTCTACT CTCAAGGCCA GTAATACATC GACAGCTGTG 
ACATCCGGCT CAGCACCCAG TATAGTCATG GAGAGAGGAG TGACAACCTG GAATATTCAA 
GGAGCATTTA CTACCTGCAA AATATTTGTG TATATCTCTT TATATCTATG TTTTTCTTTT 
TTTAACTTCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905233.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761093.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61567
Clone ID Related Accession (Same CDS sequence)	XM_031905233.1
Accession Version	XM_031905233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761093.1
CDS	139..4551
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905233.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905233.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905234.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761094.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X2, mRNA.

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CloneID	OXa61568
Clone ID Related Accession (Same CDS sequence)	XM_031905234.1
Accession Version	XM_031905234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761094.1
CDS	139..4551
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTAPVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905234.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905234.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905236.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761096.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa61569
Clone ID Related Accession (Same CDS sequence)	XM_031905236.1
Accession Version	XM_031905236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA CATCAATAGC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761096.1
CDS	139..4551
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSATSIAGTSSTALTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905236.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905236.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA CATCAATAGC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905237.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761097.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa61570
Clone ID Related Accession (Same CDS sequence)	XM_031905237.1
Accession Version	XM_031905237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
AATGCTCCAG TGACAAGCGC TACATCAATA ACTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761097.1
CDS	139..4551
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905237.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905237.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
AATGCTCCAG TGACAAGCGC TACATCAATA ACTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905238.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761098.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X5, mRNA.

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CloneID	OXa61571
Clone ID Related Accession (Same CDS sequence)	XM_031905238.1
Accession Version	XM_031905238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761098.1
CDS	139..4551
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALTAPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905238.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905238.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCACTCAC TGCTTCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
GGCACTGCAG TCAATACAGC AACTCTAGCA GCAGCTGGGA CATCCGGCAC TGCTCTCTCT 
ACTCTAAACA CAAGTAGCAC GTCATCAGCT GCGACATCCG GCACTGCTCT CTCTACTCTC 
AAGACAAGTA CTACACCAAC AGATGTGATA TCCAGCTCAG CACCCAGTAT GGGCACGGAG 
AGAGGAATGA CATGGAATAT TCAAGGAACA TTTACTACCT GCAAAATATA TGTGTATATA 
CTGTACCTTT ATATCTATGG TTTTCTTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905239.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761099.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X6, mRNA.

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CloneID	OXa61572
Clone ID Related Accession (Same CDS sequence)	XM_031905239.1
Accession Version	XM_031905239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCAGTCAAT 
ACAGCAACTC TAGCAGCAGC TGGGACATCC GGCACTGCTC TCTCTACTCT AAACACAAGT 
AGCACGTCAT CAGCTGCGAC ATCCGGCACT GCTCTCTCTA CTCTCAAGAC AAGTACTACA 
CCAACAGATG TGATATCCAG CTCAGCACCC AGTATGGGCA CGGAGAGAGG AATGACATGG 
AATATTCAAG GAACATTTAC TACCTGCAAA ATATATGTGT ATATACTGTA CCTTTATATC 
TATGGTTTTC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761099.1
CDS	139..4536
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905239.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905239.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCAGTCAAT 
ACAGCAACTC TAGCAGCAGC TGGGACATCC GGCACTGCTC TCTCTACTCT AAACACAAGT 
AGCACGTCAT CAGCTGCGAC ATCCGGCACT GCTCTCTCTA CTCTCAAGAC AAGTACTACA 
CCAACAGATG TGATATCCAG CTCAGCACCC AGTATGGGCA CGGAGAGAGG AATGACATGG 
AATATTCAAG GAACATTTAC TACCTGCAAA ATATATGTGT ATATACTGTA CCTTTATATC 
TATGGTTTTC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905240.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761100.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa61573
Clone ID Related Accession (Same CDS sequence)	XM_031905240.1
Accession Version	XM_031905240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCAGTCAAT 
ACAGCAACTC TAGCAGCAGC TGGGACATCC GGCACTGCTC TCTCTACTCT AAACACAAGT 
AGCACGTCAT CAGCTGCGAC ATCCGGCACT GCTCTCTCTA CTCTCAAGAC AAGTACTACA 
CCAACAGATG TGATATCCAG CTCAGCACCC AGTATGGGCA CGGAGAGAGG AATGACATGG 
AATATTCAAG GAACATTTAC TACCTGCAAA ATATATGTGT ATATACTGTA CCTTTATATC 
TATGGTTTTC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761100.1
CDS	139..4536
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905240.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905240.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCAGTCAAT 
ACAGCAACTC TAGCAGCAGC TGGGACATCC GGCACTGCTC TCTCTACTCT AAACACAAGT 
AGCACGTCAT CAGCTGCGAC ATCCGGCACT GCTCTCTCTA CTCTCAAGAC AAGTACTACA 
CCAACAGATG TGATATCCAG CTCAGCACCC AGTATGGGCA CGGAGAGAGG AATGACATGG 
AATATTCAAG GAACATTTAC TACCTGCAAA ATATATGTGT ATATACTGTA CCTTTATATC 
TATGGTTTTC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905241.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761101.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa61574
Clone ID Related Accession (Same CDS sequence)	XM_031905241.1
Accession Version	XM_031905241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative GPI-anchored protein pfl2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCACTGCAG TCAATACAGC AACTCTAGCA 
GCAGCTGGGA CATCCGGCAC TGCTCTCTCT ACTCTAAACA CAAGTAGCAC GTCATCAGCT 
GCGACATCCG GCACTGCTCT CTCTACTCTC AAGACAAGTA CTACACCAAC AGATGTGATA 
TCCAGCTCAG CACCCAGTAT GGGCACGGAG AGAGGAATGA CATGGAATAT TCAAGGAACA 
TTTACTACCT GCAAAATATA TGTGTATATA CTGTACCTTT ATATCTATGG TTTTCTTTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761101.1
CDS	139..4521
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTVTSATSTAGTSGTVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905241.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905241.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCACTGCAG TCAATACAGC AACTCTAGCA 
GCAGCTGGGA CATCCGGCAC TGCTCTCTCT ACTCTAAACA CAAGTAGCAC GTCATCAGCT 
GCGACATCCG GCACTGCTCT CTCTACTCTC AAGACAAGTA CTACACCAAC AGATGTGATA 
TCCAGCTCAG CACCCAGTAT GGGCACGGAG AGAGGAATGA CATGGAATAT TCAAGGAACA 
TTTACTACCT GCAAAATATA TGTGTATATA CTGTACCTTT ATATCTATGG TTTTCTTTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905242.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761102.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X9, mRNA.

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CloneID	OXa61575
Clone ID Related Accession (Same CDS sequence)	XM_031905242.1
Accession Version	XM_031905242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761102.1
CDS	139..4512
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALNAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905242.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905242.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905243.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761103.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X10, mRNA.

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CloneID	OXa61576
Clone ID Related Accession (Same CDS sequence)	XM_031905243.1
Accession Version	XM_031905243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761103.1
CDS	139..4512
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905243.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905243.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905245.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761105.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa61577
Clone ID Related Accession (Same CDS sequence)	XM_031905245.1 , XM_031905244.1
Accession Version	XM_031905245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGCACTCA CTGCTCCAGT AACAAATGGT ACATCAGCAG CAGGGACATC CGGCACTGTG 
ACAAGCGGTA CAGCAACAGC AGGGACATCC GGCACTGGCT CAGCAGTCAC TACAGCAACT 
CAAGCAGCAA CTGGGACATC CAGCACTGCA CTCACTGCTT CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCAAT GCTCCAGTGA CAAGCGCTTC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGTG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGCT ACATCAATAA CTGGGACATC CAGCACTGCA 
CTCACTGCTT CAGTGACAAG TGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCAAT 
GCTCCAGTGA CAAGCGGTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTTCATCAAT AGCTGGGACA TCCAGCACTG CACTCAATGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAATAG CTGGGACATC CAGCACTGCA CTCAATGCTC CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGCG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ATATCAATAG CTGGGACATC CAGCACTTCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGTTAC ATCAACAGCT GGGACATCCG GCACTGCACT CAATGATCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TCCAGTGACA 
AACGCTACAT CAACAGCTGG GACATCCGGC ACTGGTGTTC TGTATGAATA CGGGAACGCA 
ACTGACAGTC TAAGCACAAG AGATGATGAT GGTTCCGTGT TGGTAAATCC TACTATCAAC 
TTCCCGTTTG GGGGAAACTC CTATTCTTCT TTTTACCTGA GCAACAACGG ATACCTGGAT 
TTCAATGGAC CCGTTATTGC CCCGTTTTAT GCAGACGTTT ATAACGTAAT ATATGGTGAC 
ATTTACTACC GGCAGAGCGC TGATCCCAGC CTCCTGGCAA ACGCAACGCA AGACATCAAC 
ACCTACTTTC CAGGTTTAGG GTTCTCTGCC CAGTGGGTGT TTATAGCGAC ATGGGACAAG 
GTGCCACGGT ATCCAGGAAC TGCCAGTCAG GTGAATACGT TCCAGGTTGT TCTCATCACA 
AATGGGACCA TTTCATTTGT TCTTTTTAAC TATGCGGATA TTCAATGGAC AAATAATGCA 
CAGGCTTATT GGAACAACGG AACCCAAGTT TATCATTTAA ACACAGTAGG ATCTGTTTTC 
AATTGGACAA CATCAAGCAA CGTTAACTAT CCTGGGCGGT GGGCTTTCAT GGTAGGCAAA 
GTACAACCTG TAGCTCTCAC TACTCCAGTG ACAAGCAGTA CGTCAGCAGC GGGGACATCC 
GGCACCGCAC TGACCACTCC AGTGACAAGT GGTTCATCAA CAGTGGGGGC ATCGGGCACT 
GCACTCACTG CTCCAGTGAC AAGCGGTACA CCAACAGCGG GGACATCTGG CACTGCACTC 
ACTGCTCCAG TGACAAGCGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA ACGATACATC AACAGCTGGG ACATCCGGCA CTGGTGTTCT GTATGAATAC 
GGGAACGCAA CTGACAGTCC AAGCACAAGA ACTTATGATG GTTCCGTGTT GGTAGATCTT 
ACTGTCGCGT TCCCTCTTTT TGGAAACACC TATTCTTCTT TATACCTGTA CAACAGAGGA 
TACCTGAATT TCAATGGACC CCTTATTGTC CCATTTTATG CAGCCATTTA TAACTTAATA 
TCTGGTGACA TTTACTATCG GCAGAGCACT GATTCCAGCC TCCTGGCAAA CGCAACGCAA 
GACATCAACA CCTACTTTCC AGGTTTAGGG TTCTCTGCCC AGTTGGTGTT TATAGCGACA 
TGGGACAAGG TGCCACAGTA TCAAGGAAAT GCCAGTCAGG TGAATACGTT CCAGGTTGTT 
CTCATCACAG ATGGGACCAT TTCATTTGTT CTTTTTAACT ATGCAGATAT TCAGTGGACA 
TCTAATACAG TGGCTGGGTT GGACAGCAGA AATGCTGCAG GATATTATAT ATTAAATACA 
AGTGGATCTA CTTTCAATTG GACAAGCTCA AGTAACACTA ACTATCCTGG GCGGTGGGCT 
TTCAGAGTAG ACAGATTACA AATTGAAGTA CCCGCACTAC AGCAACCAGC AACTACAGAA 
ACCACTACTC CAATGACAAG CGGTACGTCA GCAGCGGGGA CATCTGGCAC CGGACTCATG 
ACAGCAGAGA AAAGAGCCAC ATCCGGCACT GGTGTTCTGT ATGAATACGG GAACGCAACT 
GACAGTCTAA GCGCAAGAGG TGATGGTTAT GTGTCGGTAG ATCTTACTGT CAACTTCCCT 
CTTTTTGGAA ACTCCTATTC TTCTTTTTAC CTGTACAACA GAGGATACCT GGATTTCAGT 
CGACCCTCTA TTGTCCCGTT TTATGCAGCC ATTTCTACCG CAATATCTGG TAACATTTAC 
TACCGGCAGA GCGCTGATCC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTGG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCG GATATTCAGT GGACATCTAA TGCAGAGGCT 
GCGTTGCTCA GTGGAAATGC TGCAGGATAT TATCAATTAA ATACAAGTGG ATCTTCTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGAT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCC GGCACCGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCGGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTAGA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTACTCCAG TGACAAGCGG TACGTCAGCA 
GCGGGGACAT CCGGCACCGT GACAAGCGCT ACATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761105.1
CDS	139..4512
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905245.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905245.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGCACTCA CTGCTCCAGT AACAAATGGT ACATCAGCAG CAGGGACATC CGGCACTGTG 
ACAAGCGGTA CAGCAACAGC AGGGACATCC GGCACTGGCT CAGCAGTCAC TACAGCAACT 
CAAGCAGCAA CTGGGACATC CAGCACTGCA CTCACTGCTT CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCAAT GCTCCAGTGA CAAGCGCTTC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGTG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGCT ACATCAATAA CTGGGACATC CAGCACTGCA 
CTCACTGCTT CAGTGACAAG TGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCAAT 
GCTCCAGTGA CAAGCGGTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTTCATCAAT AGCTGGGACA TCCAGCACTG CACTCAATGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAATAG CTGGGACATC CAGCACTGCA CTCAATGCTC CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGCG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ATATCAATAG CTGGGACATC CAGCACTTCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGTTAC ATCAACAGCT GGGACATCCG GCACTGCACT CAATGATCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TCCAGTGACA 
AACGCTACAT CAACAGCTGG GACATCCGGC ACTGGTGTTC TGTATGAATA CGGGAACGCA 
ACTGACAGTC TAAGCACAAG AGATGATGAT GGTTCCGTGT TGGTAAATCC TACTATCAAC 
TTCCCGTTTG GGGGAAACTC CTATTCTTCT TTTTACCTGA GCAACAACGG ATACCTGGAT 
TTCAATGGAC CCGTTATTGC CCCGTTTTAT GCAGACGTTT ATAACGTAAT ATATGGTGAC 
ATTTACTACC GGCAGAGCGC TGATCCCAGC CTCCTGGCAA ACGCAACGCA AGACATCAAC 
ACCTACTTTC CAGGTTTAGG GTTCTCTGCC CAGTGGGTGT TTATAGCGAC ATGGGACAAG 
GTGCCACGGT ATCCAGGAAC TGCCAGTCAG GTGAATACGT TCCAGGTTGT TCTCATCACA 
AATGGGACCA TTTCATTTGT TCTTTTTAAC TATGCGGATA TTCAATGGAC AAATAATGCA 
CAGGCTTATT GGAACAACGG AACCCAAGTT TATCATTTAA ACACAGTAGG ATCTGTTTTC 
AATTGGACAA CATCAAGCAA CGTTAACTAT CCTGGGCGGT GGGCTTTCAT GGTAGGCAAA 
GTACAACCTG TAGCTCTCAC TACTCCAGTG ACAAGCAGTA CGTCAGCAGC GGGGACATCC 
GGCACCGCAC TGACCACTCC AGTGACAAGT GGTTCATCAA CAGTGGGGGC ATCGGGCACT 
GCACTCACTG CTCCAGTGAC AAGCGGTACA CCAACAGCGG GGACATCTGG CACTGCACTC 
ACTGCTCCAG TGACAAGCGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA ACGATACATC AACAGCTGGG ACATCCGGCA CTGGTGTTCT GTATGAATAC 
GGGAACGCAA CTGACAGTCC AAGCACAAGA ACTTATGATG GTTCCGTGTT GGTAGATCTT 
ACTGTCGCGT TCCCTCTTTT TGGAAACACC TATTCTTCTT TATACCTGTA CAACAGAGGA 
TACCTGAATT TCAATGGACC CCTTATTGTC CCATTTTATG CAGCCATTTA TAACTTAATA 
TCTGGTGACA TTTACTATCG GCAGAGCACT GATTCCAGCC TCCTGGCAAA CGCAACGCAA 
GACATCAACA CCTACTTTCC AGGTTTAGGG TTCTCTGCCC AGTTGGTGTT TATAGCGACA 
TGGGACAAGG TGCCACAGTA TCAAGGAAAT GCCAGTCAGG TGAATACGTT CCAGGTTGTT 
CTCATCACAG ATGGGACCAT TTCATTTGTT CTTTTTAACT ATGCAGATAT TCAGTGGACA 
TCTAATACAG TGGCTGGGTT GGACAGCAGA AATGCTGCAG GATATTATAT ATTAAATACA 
AGTGGATCTA CTTTCAATTG GACAAGCTCA AGTAACACTA ACTATCCTGG GCGGTGGGCT 
TTCAGAGTAG ACAGATTACA AATTGAAGTA CCCGCACTAC AGCAACCAGC AACTACAGAA 
ACCACTACTC CAATGACAAG CGGTACGTCA GCAGCGGGGA CATCTGGCAC CGGACTCATG 
ACAGCAGAGA AAAGAGCCAC ATCCGGCACT GGTGTTCTGT ATGAATACGG GAACGCAACT 
GACAGTCTAA GCGCAAGAGG TGATGGTTAT GTGTCGGTAG ATCTTACTGT CAACTTCCCT 
CTTTTTGGAA ACTCCTATTC TTCTTTTTAC CTGTACAACA GAGGATACCT GGATTTCAGT 
CGACCCTCTA TTGTCCCGTT TTATGCAGCC ATTTCTACCG CAATATCTGG TAACATTTAC 
TACCGGCAGA GCGCTGATCC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTGG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCG GATATTCAGT GGACATCTAA TGCAGAGGCT 
GCGTTGCTCA GTGGAAATGC TGCAGGATAT TATCAATTAA ATACAAGTGG ATCTTCTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGAT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCC GGCACCGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCGGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTAGA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTACTCCAG TGACAAGCGG TACGTCAGCA 
GCGGGGACAT CCGGCACCGT GACAAGCGCT ACATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905244.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761104.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa61577
Clone ID Related Accession (Same CDS sequence)	XM_031905245.1 , XM_031905244.1
Accession Version	XM_031905244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGCACTCA CTGCTCCAGT AACAAATGGT ACATCAGCAG CAGGGACATC CGGCACTGTG 
ACAAGCGGTA CAGCAACAGC AGGGACATCC GGCACTGGCT CAGCAGTCAC TACAGCAACT 
CAAGCAGCAA CTGGGACATC CAGCACTGCA CTCACTGCTT CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCAAT GCTCCAGTGA CAAGCGCTTC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGTG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGCT ACATCAATAA CTGGGACATC CAGCACTGCA 
CTCACTGCTT CAGTGACAAG TGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCAAT 
GCTCCAGTGA CAAGCGGTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTTCATCAAT AGCTGGGACA TCCAGCACTG CACTCAATGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAATAG CTGGGACATC CAGCACTGCA CTCAATGCTC CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGCG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ATATCAATAG CTGGGACATC CAGCACTTCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGTTAC ATCAACAGCT GGGACATCCG GCACTGCACT CAATGATCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TCCAGTGACA 
AACGCTACAT CAACAGCTGG GACATCCGGC ACTGGTGTTC TGTATGAATA CGGGAACGCA 
ACTGACAGTC TAAGCACAAG AGATGATGAT GGTTCCGTGT TGGTAAATCC TACTATCAAC 
TTCCCGTTTG GGGGAAACTC CTATTCTTCT TTTTACCTGA GCAACAACGG ATACCTGGAT 
TTCAATGGAC CCGTTATTGC CCCGTTTTAT GCAGACGTTT ATAACGTAAT ATATGGTGAC 
ATTTACTACC GGCAGAGCGC TGATCCCAGC CTCCTGGCAA ACGCAACGCA AGACATCAAC 
ACCTACTTTC CAGGTTTAGG GTTCTCTGCC CAGTGGGTGT TTATAGCGAC ATGGGACAAG 
GTGCCACGGT ATCCAGGAAC TGCCAGTCAG GTGAATACGT TCCAGGTTGT TCTCATCACA 
AATGGGACCA TTTCATTTGT TCTTTTTAAC TATGCGGATA TTCAATGGAC AAATAATGCA 
CAGGCTTATT GGAACAACGG AACCCAAGTT TATCATTTAA ACACAGTAGG ATCTGTTTTC 
AATTGGACAA CATCAAGCAA CGTTAACTAT CCTGGGCGGT GGGCTTTCAT GGTAGGCAAA 
GTACAACCTG TAGCTCTCAC TACTCCAGTG ACAAGCAGTA CGTCAGCAGC GGGGACATCC 
GGCACCGCAC TGACCACTCC AGTGACAAGT GGTTCATCAA CAGTGGGGGC ATCGGGCACT 
GCACTCACTG CTCCAGTGAC AAGCGGTACA CCAACAGCGG GGACATCTGG CACTGCACTC 
ACTGCTCCAG TGACAAGCGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA ACGATACATC AACAGCTGGG ACATCCGGCA CTGGTGTTCT GTATGAATAC 
GGGAACGCAA CTGACAGTCC AAGCACAAGA ACTTATGATG GTTCCGTGTT GGTAGATCTT 
ACTGTCGCGT TCCCTCTTTT TGGAAACACC TATTCTTCTT TATACCTGTA CAACAGAGGA 
TACCTGAATT TCAATGGACC CCTTATTGTC CCATTTTATG CAGCCATTTA TAACTTAATA 
TCTGGTGACA TTTACTATCG GCAGAGCACT GATTCCAGCC TCCTGGCAAA CGCAACGCAA 
GACATCAACA CCTACTTTCC AGGTTTAGGG TTCTCTGCCC AGTTGGTGTT TATAGCGACA 
TGGGACAAGG TGCCACAGTA TCAAGGAAAT GCCAGTCAGG TGAATACGTT CCAGGTTGTT 
CTCATCACAG ATGGGACCAT TTCATTTGTT CTTTTTAACT ATGCAGATAT TCAGTGGACA 
TCTAATACAG TGGCTGGGTT GGACAGCAGA AATGCTGCAG GATATTATAT ATTAAATACA 
AGTGGATCTA CTTTCAATTG GACAAGCTCA AGTAACACTA ACTATCCTGG GCGGTGGGCT 
TTCAGAGTAG ACAGATTACA AATTGAAGTA CCCGCACTAC AGCAACCAGC AACTACAGAA 
ACCACTACTC CAATGACAAG CGGTACGTCA GCAGCGGGGA CATCTGGCAC CGGACTCATG 
ACAGCAGAGA AAAGAGCCAC ATCCGGCACT GGTGTTCTGT ATGAATACGG GAACGCAACT 
GACAGTCTAA GCGCAAGAGG TGATGGTTAT GTGTCGGTAG ATCTTACTGT CAACTTCCCT 
CTTTTTGGAA ACTCCTATTC TTCTTTTTAC CTGTACAACA GAGGATACCT GGATTTCAGT 
CGACCCTCTA TTGTCCCGTT TTATGCAGCC ATTTCTACCG CAATATCTGG TAACATTTAC 
TACCGGCAGA GCGCTGATCC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTGG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCG GATATTCAGT GGACATCTAA TGCAGAGGCT 
GCGTTGCTCA GTGGAAATGC TGCAGGATAT TATCAATTAA ATACAAGTGG ATCTTCTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGAT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCC GGCACCGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCGGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTAGA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTACTCCAG TGACAAGCGG TACGTCAGCA 
GCGGGGACAT CCGGCACCGT GACAAGCGCT ACATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761104.1
CDS	139..4512
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905244.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905244.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGCACTCA CTGCTCCAGT AACAAATGGT ACATCAGCAG CAGGGACATC CGGCACTGTG 
ACAAGCGGTA CAGCAACAGC AGGGACATCC GGCACTGGCT CAGCAGTCAC TACAGCAACT 
CAAGCAGCAA CTGGGACATC CAGCACTGCA CTCACTGCTT CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCAAT GCTCCAGTGA CAAGCGCTTC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGTG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGCT ACATCAATAA CTGGGACATC CAGCACTGCA 
CTCACTGCTT CAGTGACAAG TGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCAAT 
GCTCCAGTGA CAAGCGGTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTTCATCAAT AGCTGGGACA TCCAGCACTG CACTCAATGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAATAG CTGGGACATC CAGCACTGCA CTCAATGCTC CAGTGACAAG CGCTACATCA 
ACAGCTGGGA CATCCAGCAC TGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAATAGCT 
GGGACATCCA GCACTGCACT CACTGCTTCA GTGACAAGCG CTACATCAAT AGCTGGGACA 
TCCAGCACTG CACTCACTGC TTCAGTGACA AGTGCTACAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ATATCAATAG CTGGGACATC CAGCACTTCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGTTAC ATCAACAGCT GGGACATCCG GCACTGCACT CAATGATCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TCCAGTGACA 
AACGCTACAT CAACAGCTGG GACATCCGGC ACTGGTGTTC TGTATGAATA CGGGAACGCA 
ACTGACAGTC TAAGCACAAG AGATGATGAT GGTTCCGTGT TGGTAAATCC TACTATCAAC 
TTCCCGTTTG GGGGAAACTC CTATTCTTCT TTTTACCTGA GCAACAACGG ATACCTGGAT 
TTCAATGGAC CCGTTATTGC CCCGTTTTAT GCAGACGTTT ATAACGTAAT ATATGGTGAC 
ATTTACTACC GGCAGAGCGC TGATCCCAGC CTCCTGGCAA ACGCAACGCA AGACATCAAC 
ACCTACTTTC CAGGTTTAGG GTTCTCTGCC CAGTGGGTGT TTATAGCGAC ATGGGACAAG 
GTGCCACGGT ATCCAGGAAC TGCCAGTCAG GTGAATACGT TCCAGGTTGT TCTCATCACA 
AATGGGACCA TTTCATTTGT TCTTTTTAAC TATGCGGATA TTCAATGGAC AAATAATGCA 
CAGGCTTATT GGAACAACGG AACCCAAGTT TATCATTTAA ACACAGTAGG ATCTGTTTTC 
AATTGGACAA CATCAAGCAA CGTTAACTAT CCTGGGCGGT GGGCTTTCAT GGTAGGCAAA 
GTACAACCTG TAGCTCTCAC TACTCCAGTG ACAAGCAGTA CGTCAGCAGC GGGGACATCC 
GGCACCGCAC TGACCACTCC AGTGACAAGT GGTTCATCAA CAGTGGGGGC ATCGGGCACT 
GCACTCACTG CTCCAGTGAC AAGCGGTACA CCAACAGCGG GGACATCTGG CACTGCACTC 
ACTGCTCCAG TGACAAGCGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA ACGATACATC AACAGCTGGG ACATCCGGCA CTGGTGTTCT GTATGAATAC 
GGGAACGCAA CTGACAGTCC AAGCACAAGA ACTTATGATG GTTCCGTGTT GGTAGATCTT 
ACTGTCGCGT TCCCTCTTTT TGGAAACACC TATTCTTCTT TATACCTGTA CAACAGAGGA 
TACCTGAATT TCAATGGACC CCTTATTGTC CCATTTTATG CAGCCATTTA TAACTTAATA 
TCTGGTGACA TTTACTATCG GCAGAGCACT GATTCCAGCC TCCTGGCAAA CGCAACGCAA 
GACATCAACA CCTACTTTCC AGGTTTAGGG TTCTCTGCCC AGTTGGTGTT TATAGCGACA 
TGGGACAAGG TGCCACAGTA TCAAGGAAAT GCCAGTCAGG TGAATACGTT CCAGGTTGTT 
CTCATCACAG ATGGGACCAT TTCATTTGTT CTTTTTAACT ATGCAGATAT TCAGTGGACA 
TCTAATACAG TGGCTGGGTT GGACAGCAGA AATGCTGCAG GATATTATAT ATTAAATACA 
AGTGGATCTA CTTTCAATTG GACAAGCTCA AGTAACACTA ACTATCCTGG GCGGTGGGCT 
TTCAGAGTAG ACAGATTACA AATTGAAGTA CCCGCACTAC AGCAACCAGC AACTACAGAA 
ACCACTACTC CAATGACAAG CGGTACGTCA GCAGCGGGGA CATCTGGCAC CGGACTCATG 
ACAGCAGAGA AAAGAGCCAC ATCCGGCACT GGTGTTCTGT ATGAATACGG GAACGCAACT 
GACAGTCTAA GCGCAAGAGG TGATGGTTAT GTGTCGGTAG ATCTTACTGT CAACTTCCCT 
CTTTTTGGAA ACTCCTATTC TTCTTTTTAC CTGTACAACA GAGGATACCT GGATTTCAGT 
CGACCCTCTA TTGTCCCGTT TTATGCAGCC ATTTCTACCG CAATATCTGG TAACATTTAC 
TACCGGCAGA GCGCTGATCC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTGG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCG GATATTCAGT GGACATCTAA TGCAGAGGCT 
GCGTTGCTCA GTGGAAATGC TGCAGGATAT TATCAATTAA ATACAAGTGG ATCTTCTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGAT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCC GGCACCGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCGGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTAGA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTACTCCAG TGACAAGCGG TACGTCAGCA 
GCGGGGACAT CCGGCACCGT GACAAGCGCT ACATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGGTAT GTCAGCAGCT GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG CTATATCAAC AGCTGGGACA TCCAGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCACTCA CTGCTTCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CGGCACTGCA GTCAATACAG CAACTCTAGC AGCAGCTGGG 
ACATCCGGCA CTGCTCTCTC TACTCTAAAC ACAAGTAGCA CGTCATCAGC TGCGACATCC 
GGCACTGCTC TCTCTACTCT CAAGACAAGT ACTACACCAA CAGATGTGAT ATCCAGCTCA 
GCACCCAGTA TGGGCACGGA GAGAGGAATG ACATGGAATA TTCAAGGAAC ATTTACTACC 
TGCAAAATAT ATGTGTATAT ACTGTACCTT TATATCTATG GTTTTCTTTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905246.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761106.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X13, mRNA.

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CloneID	OXa61578
Clone ID Related Accession (Same CDS sequence)	XM_031905246.1
Accession Version	XM_031905246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761106.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905246.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905246.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905247.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761107.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X14, mRNA.

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CloneID	OXa61579
Clone ID Related Accession (Same CDS sequence)	XM_031905247.1
Accession Version	XM_031905247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTACT 
CCAGTGACAA GCGCTACATC AATAACTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761107.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905247.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905247.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTACT 
CCAGTGACAA GCGCTACATC AATAACTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905248.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761108.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OXa61580
Clone ID Related Accession (Same CDS sequence)	XM_031905248.1
Accession Version	XM_031905248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761108.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905248.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905248.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905249.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761109.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa61581
Clone ID Related Accession (Same CDS sequence)	XM_031905249.1
Accession Version	XM_031905249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761109.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905249.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905249.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905250.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761110.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X17, mRNA.

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CloneID	OXa61582
Clone ID Related Accession (Same CDS sequence)	XM_031905250.1
Accession Version	XM_031905250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761110.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTAPVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905250.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905250.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905251.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761111.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X18, mRNA.

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CloneID	OXa61583
Clone ID Related Accession (Same CDS sequence)	XM_031905251.1
Accession Version	XM_031905251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761111.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905251.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905251.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905252.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761112.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OXa61584
Clone ID Related Accession (Same CDS sequence)	XM_031905252.1
Accession Version	XM_031905252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
AATGCTCCAG TGACAAGCGC TACATCAATA ACTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761112.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSITGTSSTALTASVTSATSTAGTSSTALTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905252.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905252.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
AATGCTCCAG TGACAAGCGC TACATCAATA ACTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905253.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761113.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OXa61585
Clone ID Related Accession (Same CDS sequence)	XM_031905253.1
Accession Version	XM_031905253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761113.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905253.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905253.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905254.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761114.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X21, mRNA.

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CloneID	OXa61586
Clone ID Related Accession (Same CDS sequence)	XM_031905254.1
Accession Version	XM_031905254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761114.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTAPVTSATSTAGTSSTALTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905254.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905254.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905255.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761115.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X22, mRNA.

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CloneID	OXa61587
Clone ID Related Accession (Same CDS sequence)	XM_031905255.1
Accession Version	XM_031905255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761115.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905255.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905255.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905256.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761116.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OXa61588
Clone ID Related Accession (Same CDS sequence)	XM_031905256.1
Accession Version	XM_031905256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761116.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905256.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905256.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905257.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761117.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OXa61589
Clone ID Related Accession (Same CDS sequence)	XM_031905257.1
Accession Version	XM_031905257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized threonine-rich GPI-anchored glycoprotein PJ4664.02-like isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761117.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905257.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905257.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905258.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761118.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X25, mRNA.

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CloneID	OXa61590
Clone ID Related Accession (Same CDS sequence)	XM_031905258.1
Accession Version	XM_031905258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761118.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTAPVTSATSTAGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905258.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905258.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
CCAGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905259.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761119.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X26, mRNA.

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CloneID	OXa61591
Clone ID Related Accession (Same CDS sequence)	XM_031905259.1
Accession Version	XM_031905259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTTCATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGTGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTACT 
CCAGTGACAA GCGCTACATC AATAACTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761119.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905259.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905259.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTTCATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGTGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTACT 
CCAGTGACAA GCGCTACATC AATAACTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905260.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761120.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OXa61592
Clone ID Related Accession (Same CDS sequence)	XM_031905260.1
Accession Version	XM_031905260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGTGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTACT 
CCAGTGACAA GCGCTACATC AATAACTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761120.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905260.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905260.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGTGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCACTACT 
CCAGTGACAA GCGCTACATC AATAACTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGTGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905261.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761121.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OXa61593
Clone ID Related Accession (Same CDS sequence)	XM_031905261.1
Accession Version	XM_031905261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761121.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTAPVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905261.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905261.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCAATGCTCC AGTGACAAGC 
GGTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCACTG CTCCAGTGAC AAGCGCTTCA 
TCAATAGCTG GGACATCCAG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905262.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761122.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X29, mRNA.

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CloneID	OXa61594
Clone ID Related Accession (Same CDS sequence)	XM_031905262.1
Accession Version	XM_031905262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761122.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905262.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905262.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCGGCCCT GCACTCACTG CTCCAGTGAC AAGCGGTATG 
TCAGCAGCTG GGACATCCGG CACCGCACTC ACTGCTCCAG TGACAAGCGC TATATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905263.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761123.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X30, mRNA.

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CloneID	OXa61595
Clone ID Related Accession (Same CDS sequence)	XM_031905263.1
Accession Version	XM_031905263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761123.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905263.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905263.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905264.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761124.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OXa61596
Clone ID Related Accession (Same CDS sequence)	XM_031905264.1
Accession Version	XM_031905264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761124.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905264.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905264.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905265.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761125.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OXa61597
Clone ID Related Accession (Same CDS sequence)	XM_031905265.1
Accession Version	XM_031905265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTACATC AATAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761125.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSATSIAGTSSTALTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905265.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905265.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTACATC AATAGCTGGG ACATCCAGCA CTGCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905266.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761126.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X33, mRNA.

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CloneID	OXa61598
Clone ID Related Accession (Same CDS sequence)	XM_031905266.1
Accession Version	XM_031905266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761126.1
CDS	139..4497
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905266.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905266.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905267.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761127.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X34, mRNA.

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CloneID	OXa61599
Clone ID Related Accession (Same CDS sequence)	XM_031905267.1
Accession Version	XM_031905267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761127.1
CDS	157..4515
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905267.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905267.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCACTGCT CCAGTGACAA GCGCTACATC AATAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905268.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761128.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OXa61600
Clone ID Related Accession (Same CDS sequence)	XM_031905268.1
Accession Version	XM_031905268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTACA TCAATAACTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761128.1
CDS	160..4518
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALNAPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905268.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905268.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTACA TCAATAACTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905269.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761129.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OXa61601
Clone ID Related Accession (Same CDS sequence)	XM_031905269.1
Accession Version	XM_031905269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761129.1
CDS	159..4517
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905269.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905269.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905270.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761130.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X37, mRNA.

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CloneID	OXa61602
Clone ID Related Accession (Same CDS sequence)	XM_031905270.1
Accession Version	XM_031905270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761130.1
CDS	171..4529
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905270.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905270.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905271.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761131.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X38, mRNA.

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CloneID	OXa61603
Clone ID Related Accession (Same CDS sequence)	XM_031905271.1
Accession Version	XM_031905271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mucin-5AC-like isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761131.1
CDS	171..4529
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTVTSATSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905271.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905271.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAA TGCTCCAGTG ACAAGCGCTA CATCAACAGC TGGGACATCC 
AGCACTGCAC TCACTGCTCC AGTGACAAGC GCTACATCAA TAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTTCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAACA GCTGGGACAT CCAGCACTGC ACTCAATGCT 
CCAGTGACAA GCGCTATATC AATAGCTGGG ACATCCAGCA CTTCACTCAC TGCTTCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC AGCACTGCAC TCACTGCTCC AGTGACAAGC 
GTTACATCAA CAGCTGGGAC ATCCGGCACT GCACTCAATG ATCCAGTGAC AAGCGCTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAACGC TACATCAACA 
GCTGGGACAT CCGGCACTGG TGTTCTGTAT GAATACGGGA ACGCAACTGA CAGTCTAAGC 
ACAAGAGATG ATGATGGTTC CGTGTTGGTA AATCCTACTA TCAACTTCCC GTTTGGGGGA 
AACTCCTATT CTTCTTTTTA CCTGAGCAAC AACGGATACC TGGATTTCAA TGGACCCGTT 
ATTGCCCCGT TTTATGCAGA CGTTTATAAC GTAATATATG GTGACATTTA CTACCGGCAG 
AGCGCTGATC CCAGCCTCCT GGCAAACGCA ACGCAAGACA TCAACACCTA CTTTCCAGGT 
TTAGGGTTCT CTGCCCAGTG GGTGTTTATA GCGACATGGG ACAAGGTGCC ACGGTATCCA 
GGAACTGCCA GTCAGGTGAA TACGTTCCAG GTTGTTCTCA TCACAAATGG GACCATTTCA 
TTTGTTCTTT TTAACTATGC GGATATTCAA TGGACAAATA ATGCACAGGC TTATTGGAAC 
AACGGAACCC AAGTTTATCA TTTAAACACA GTAGGATCTG TTTTCAATTG GACAACATCA 
AGCAACGTTA ACTATCCTGG GCGGTGGGCT TTCATGGTAG GCAAAGTACA ACCTGTAGCT 
CTCACTACTC CAGTGACAAG CAGTACGTCA GCAGCGGGGA CATCCGGCAC CGCACTGACC 
ACTCCAGTGA CAAGTGGTTC ATCAACAGTG GGGGCATCGG GCACTGCACT CACTGCTCCA 
GTGACAAGCG GTACACCAAC AGCGGGGACA TCTGGCACTG CACTCACTGC TCCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCAGC ACTGCACTCA CTGCTCCAGT GACAAACGAT 
ACATCAACAG CTGGGACATC CGGCACTGGT GTTCTGTATG AATACGGGAA CGCAACTGAC 
AGTCCAAGCA CAAGAACTTA TGATGGTTCC GTGTTGGTAG ATCTTACTGT CGCGTTCCCT 
CTTTTTGGAA ACACCTATTC TTCTTTATAC CTGTACAACA GAGGATACCT GAATTTCAAT 
GGACCCCTTA TTGTCCCATT TTATGCAGCC ATTTATAACT TAATATCTGG TGACATTTAC 
TATCGGCAGA GCACTGATTC CAGCCTCCTG GCAAACGCAA CGCAAGACAT CAACACCTAC 
TTTCCAGGTT TAGGGTTCTC TGCCCAGTTG GTGTTTATAG CGACATGGGA CAAGGTGCCA 
CAGTATCAAG GAAATGCCAG TCAGGTGAAT ACGTTCCAGG TTGTTCTCAT CACAGATGGG 
ACCATTTCAT TTGTTCTTTT TAACTATGCA GATATTCAGT GGACATCTAA TACAGTGGCT 
GGGTTGGACA GCAGAAATGC TGCAGGATAT TATATATTAA ATACAAGTGG ATCTACTTTC 
AATTGGACAA GCTCAAGTAA CACTAACTAT CCTGGGCGGT GGGCTTTCAG AGTAGACAGA 
TTACAAATTG AAGTACCCGC ACTACAGCAA CCAGCAACTA CAGAAACCAC TACTCCAATG 
ACAAGCGGTA CGTCAGCAGC GGGGACATCT GGCACCGGAC TCATGACAGC AGAGAAAAGA 
GCCACATCCG GCACTGGTGT TCTGTATGAA TACGGGAACG CAACTGACAG TCTAAGCGCA 
AGAGGTGATG GTTATGTGTC GGTAGATCTT ACTGTCAACT TCCCTCTTTT TGGAAACTCC 
TATTCTTCTT TTTACCTGTA CAACAGAGGA TACCTGGATT TCAGTCGACC CTCTATTGTC 
CCGTTTTATG CAGCCATTTC TACCGCAATA TCTGGTAACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACAGTA TCAAGGAAAT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAG ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAGTGGACA TCTAATGCAG AGGCTGCGTT GCTCAGTGGA 
AATGCTGCAG GATATTATCA ATTAAATACA AGTGGATCTT CTTTCAATTG GACAAGCTCA 
AGTAACACTA ACTATCCTGG GCGATGGGCT TTCAGAGTAG ACAGATTACA AATTGAAGTA 
CCCGCACTAC AGCAACCAGC AACTACAGAA ACCACTACTC CAATGACAAG CGGTACGTCA 
GCAGCGGGGA CATCCGGCAC CGCACTCACT GCTCCAGTGA CAAGCGCTAC ATCAACAGCG 
GGGACATCCG GCACTGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCACTAC TCCAGTGACA AGCGGTACGT CAGCAGCGGG GACATCCGGC 
ACCGTGACAA GCGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TGCTCCAGTG 
ACAAGCGCTA CATCAACAGC TGGGACATCC GGCCCTGCAC TCACTGCTCC AGTGACAAGC 
GGTATGTCAG CAGCTGGGAC ATCCGGCACC GCACTCACTG CTCCAGTGAC AAGCGCTATA 
TCAACAGCTG GGACATCCAG CACTGCACTC ACTGCTCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT TCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCGGCA CTGCAGTCAA TACAGCAACT CTAGCAGCAG CTGGGACATC CGGCACTGCT 
CTCTCTACTC TAAACACAAG TAGCACGTCA TCAGCTGCGA CATCCGGCAC TGCTCTCTCT 
ACTCTCAAGA CAAGTACTAC ACCAACAGAT GTGATATCCA GCTCAGCACC CAGTATGGGC 
ACGGAGAGAG GAATGACATG GAATATTCAA GGAACATTTA CTACCTGCAA AATATATGTG 
TATATACTGT ACCTTTATAT CTATGGTTTT CTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905272.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761132.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OXa61604
Clone ID Related Accession (Same CDS sequence)	XM_031905272.1
Accession Version	XM_031905272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761132.1
CDS	139..4458
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALNAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905272.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905272.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905273.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761133.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OXa61605
Clone ID Related Accession (Same CDS sequence)	XM_031905273.1
Accession Version	XM_031905273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761133.1
CDS	139..4458
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTALTAPVTSATSTAGTSGPALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905273.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905273.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905274.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761134.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X41, mRNA.

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CloneID	OXa61606
Clone ID Related Accession (Same CDS sequence)	XM_031905274.1
Accession Version	XM_031905274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X41
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGGTATGTCA GCAGCTGGGA CATCCGGCAC CGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761134.1
CDS	139..4458
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905274.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905274.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGGTATGTCA GCAGCTGGGA CATCCGGCAC CGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905275.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761135.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X42, mRNA.

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CloneID	OXa61607
Clone ID Related Accession (Same CDS sequence)	XM_031905275.1
Accession Version	XM_031905275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X42
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCAATGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGGTATGTCA GCAGCTGGGA CATCCGGCAC CGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761135.1
CDS	139..4458
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALNAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905275.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905275.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCAATGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGGTATGTCA GCAGCTGGGA CATCCGGCAC CGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905276.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761136.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X43, mRNA.

Price & Availability↑

CloneID	OXa61608
Clone ID Related Accession (Same CDS sequence)	XM_031905276.1
Accession Version	XM_031905276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCAATGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGGTATGTCA GCAGCTGGGA CATCCGGCAC CGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761136.1
CDS	139..4458
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSARSTAGTSSTALNAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905276.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905276.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTAGATCAAC AGCTGGGACA 
TCCAGCACTG CACTCAATGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGGTATGTCA GCAGCTGGGA CATCCGGCAC CGCACTCACT 
GCTCCAGTGA CAAGCGCTAT ATCAACAGCT GGGACATCCA GCACTGCACT CACTGCTCCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CACTCACTGC TTCAGTGACA 
AGCGCTACAT CAACAGCTGG GACATCCGGC ACTGCAGTCA ATACAGCAAC TCTAGCAGCA 
GCTGGGACAT CCGGCACTGC TCTCTCTACT CTAAACACAA GTAGCACGTC ATCAGCTGCG 
ACATCCGGCA CTGCTCTCTC TACTCTCAAG ACAAGTACTA CACCAACAGA TGTGATATCC 
AGCTCAGCAC CCAGTATGGG CACGGAGAGA GGAATGACAT GGAATATTCA AGGAACATTT 
ACTACCTGCA AAATATATGT GTATATACTG TACCTTTATA TCTATGGTTT TCTTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905277.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761137.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X44, mRNA.

Price & Availability↑

CloneID	OXa61609
Clone ID Related Accession (Same CDS sequence)	XM_031905277.1
Accession Version	XM_031905277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative GPI-anchored protein pfl2 isoform X44
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGCTATATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761137.1
CDS	139..4404
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTAPVTSATSTAGTSGPALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905277.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905277.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCCCTGCA 
CTCACTGCTC CAGTGACAAG CGCTATATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905278.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761138.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X45, mRNA.

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CloneID	OXa61610
Clone ID Related Accession (Same CDS sequence)	XM_031905278.1
Accession Version	XM_031905278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative GPI-anchored protein pfl2 isoform X45
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
CCTGCACTCA CTGCTCCAGT GACAAGCGGT ATGTCAGCAG CTGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTATATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761138.1
CDS	139..4404
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSGMSAAGTSGTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905278.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905278.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
CCTGCACTCA CTGCTCCAGT GACAAGCGGT ATGTCAGCAG CTGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTATATCA ACAGCTGGGA CATCCAGCAC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905279.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761139.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X46, mRNA.

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CloneID	OXa61611
Clone ID Related Accession (Same CDS sequence)	XM_031905279.1
Accession Version	XM_031905279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative GPI-anchored protein pfl2 isoform X46
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761139.1
CDS	139..4404
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTTPVTSGTSAAGTSGTALTAPVTSATSTAGTSGPALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905279.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905279.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTACTCCAGT GACAAGCGGT ACGTCAGCAG CGGGGACATC CGGCACCGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905280.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761140.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X47, mRNA.

Price & Availability↑

CloneID	OXa61612
Clone ID Related Accession (Same CDS sequence)	XM_031905280.1
Accession Version	XM_031905280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative GPI-anchored protein pfl2 isoform X47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761140.1
CDS	139..4404
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALNAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905280.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905280.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA ATGCTCCAGT GACAAGCGCT ACATCAACAG CTGGGACATC CGGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCCC TGCACTCACT 
GCTCCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCACT CACTGCTTCA 
GTGACAAGCG CTACATCAAC AGCTGGGACA TCCGGCACTG CAGTCAATAC AGCAACTCTA 
GCAGCAGCTG GGACATCCGG CACTGCTCTC TCTACTCTAA ACACAAGTAG CACGTCATCA 
GCTGCGACAT CCGGCACTGC TCTCTCTACT CTCAAGACAA GTACTACACC AACAGATGTG 
ATATCCAGCT CAGCACCCAG TATGGGCACG GAGAGAGGAA TGACATGGAA TATTCAAGGA 
ACATTTACTA CCTGCAAAAT ATATGTGTAT ATACTGTACC TTTATATCTA TGGTTTTCTT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905281.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761141.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X48, mRNA.

Price & Availability↑

CloneID	OXa61613
Clone ID Related Accession (Same CDS sequence)	XM_031905281.1
Accession Version	XM_031905281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X48
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
CCTGCACTCA CTGCTCCAGT GACAAGCGCT ATATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCACTCACT 
GCTTCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCAGT CAATACAGCA 
ACTCTAGCAG CAGCTGGGAC ATCCGGCACT GCTCTCTCTA CTCTAAACAC AAGTAGCACG 
TCATCAGCTG CGACATCCGG CACTGCTCTC TCTACTCTCA AGACAAGTAC TACACCAACA 
GATGTGATAT CCAGCTCAGC ACCCAGTATG GGCACGGAGA GAGGAATGAC ATGGAATATT 
CAAGGAACAT TTACTACCTG CAAAATATAT GTGTATATAC TGTACCTTTA TATCTATGGT 
TTTCTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761141.1
CDS	139..4350
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSATSTAGTSGPALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905281.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X48, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905281.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTACAT CAACAGCTGG GACATCCGGC 
CCTGCACTCA CTGCTCCAGT GACAAGCGCT ATATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCACTCACT 
GCTTCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCAGT CAATACAGCA 
ACTCTAGCAG CAGCTGGGAC ATCCGGCACT GCTCTCTCTA CTCTAAACAC AAGTAGCACG 
TCATCAGCTG CGACATCCGG CACTGCTCTC TCTACTCTCA AGACAAGTAC TACACCAACA 
GATGTGATAT CCAGCTCAGC ACCCAGTATG GGCACGGAGA GAGGAATGAC ATGGAATATT 
CAAGGAACAT TTACTACCTG CAAAATATAT GTGTATATAC TGTACCTTTA TATCTATGGT 
TTTCTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905282.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761142.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X49, mRNA.

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CloneID	OXa61614
Clone ID Related Accession (Same CDS sequence)	XM_031905282.1
Accession Version	XM_031905282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X49
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ATATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCACTCACT 
GCTTCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCAGT CAATACAGCA 
ACTCTAGCAG CAGCTGGGAC ATCCGGCACT GCTCTCTCTA CTCTAAACAC AAGTAGCACG 
TCATCAGCTG CGACATCCGG CACTGCTCTC TCTACTCTCA AGACAAGTAC TACACCAACA 
GATGTGATAT CCAGCTCAGC ACCCAGTATG GGCACGGAGA GAGGAATGAC ATGGAATATT 
CAAGGAACAT TTACTACCTG CAAAATATAT GTGTATATAC TGTACCTTTA TATCTATGGT 
TTTCTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761142.1
CDS	139..4350
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTAPVTSAISTAGTSSTALTAPVTSATSTAGTSGTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905282.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X49, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905282.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ATATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTC CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCACTCACT 
GCTTCAGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGCAGT CAATACAGCA 
ACTCTAGCAG CAGCTGGGAC ATCCGGCACT GCTCTCTCTA CTCTAAACAC AAGTAGCACG 
TCATCAGCTG CGACATCCGG CACTGCTCTC TCTACTCTCA AGACAAGTAC TACACCAACA 
GATGTGATAT CCAGCTCAGC ACCCAGTATG GGCACGGAGA GAGGAATGAC ATGGAATATT 
CAAGGAACAT TTACTACCTG CAAAATATAT GTGTATATAC TGTACCTTTA TATCTATGGT 
TTTCTTTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108645188 ( XM_031905283.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761143.1 Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X50, mRNA.

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CloneID	OXa61615
Clone ID Related Accession (Same CDS sequence)	XM_031905283.1
Accession Version	XM_031905283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine-rich adhesin for platelets-like isoform X50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ATATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCAGTCAAT 
ACAGCAACTC TAGCAGCAGC TGGGACATCC GGCACTGCTC TCTCTACTCT AAACACAAGT 
AGCACGTCAT CAGCTGCGAC ATCCGGCACT GCTCTCTCTA CTCTCAAGAC AAGTACTACA 
CCAACAGATG TGATATCCAG CTCAGCACCC AGTATGGGCA CGGAGAGAGG AATGACATGG 
AATATTCAAG GAACATTTAC TACCTGCAAA ATATATGTGT ATATACTGTA CCTTTATATC 
TATGGTTTTC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761143.1
CDS	139..4296
Translation	MDLRYTAKIFLLTYLVLGGAAQTNAQSTTVTSDTSTAGTSSTVTSATSTAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTVTNGTSAAGTSGTALTAPVTNGTSAAGTSGTVTSGTATAGTSGTGSAVTTATQAATGTSSTALTASVTSATSTAGTSSTALNAPVTSASSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALTTPVTSATSITGTSSTALTASVTSATSTAGTSSTALNAPVTSGTSTAGTSGTALTAPVTSASSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALNAPVTSATSTAGTSSTALTAPVTSATSIAGTSSTALTASVTSATSIAGTSSTALTASVTSATSTAGTSSTALNAPVTSAISIAGTSSTSLTASVTSATSTAGTSSTALTAPVTSVTSTAGTSGTALNDPVTSATSTAGTSGTALTAPVTNATSTAGTSGTGVLYEYGNATDSLSTRDDDGSVLVNPTINFPFGGNSYSSFYLSNNGYLDFNGPVIAPFYADVYNVIYGDIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPRYPGTASQVNTFQVVLITNGTISFVLFNYADIQWTNNAQAYWNNGTQVYHLNTVGSVFNWTTSSNVNYPGRWAFMVGKVQPVALTTPVTSSTSAAGTSGTALTTPVTSGSSTVGASGTALTAPVTSGTPTAGTSGTALTAPVTSATSTAGTSSTALTAPVTNDTSTAGTSGTGVLYEYGNATDSPSTRTYDGSVLVDLTVAFPLFGNTYSSLYLYNRGYLNFNGPLIVPFYAAIYNLISGDIYYRQSTDSSLLANATQDINTYFPGLGFSAQLVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNTVAGLDSRNAAGYYILNTSGSTFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTGLMTAEKRATSGTGVLYEYGNATDSLSARGDGYVSVDLTVNFPLFGNSYSSFYLYNRGYLDFSRPSIVPFYAAISTAISGNIYYRQSADPSLLANATQDINTYFPGLGFSAQWVFIATWDKVPQYQGNASQVNTFQVVLITDGTISFVLFNYADIQWTSNAEAALLSGNAAGYYQLNTSGSSFNWTSSSNTNYPGRWAFRVDRLQIEVPALQQPATTETTTPMTSGTSAAGTSGTALTAPVTSATSTAGTSGTALTAPVTSARSTAGTSSTALTAPVTSAISTAGTSSTALTASVTSATSTAGTSGTAVNTATLAAAGTSGTALSTLNTSSTSSAATSGTALSTLKTSTTPTDVISSSAPSMGTERGMTWNIQGTFTTCKIYVYILYLYIYGFLF

Target ORF information:

RefSeq Version	XM_031905283.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis putative GPI-anchored protein pfl2 (LOC108645188), transcript variant X50, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905283.1

ORF Insert Sequence:

ATGGACCTTC GTTATACTGC AAAGATTTTT CTGCTTACTT ACCTTGTGTT AGGAGGAGCA 
GCTCAAACAA ATGCACAAAG TACTACAGTG ACAAGTGATA CATCAACAGC TGGGACATCC 
AGCACTGTGA CAAGCGCTAC ATCAACAGCT GGGACATCCG GCACTGTGAC AAATGGTACG 
TCAGCAGCTG GGACATCTGG CACTGTGACA AATGGTACGT CAGCAGCTGG GACATCTGGC 
ACTGTGACAA ATGGTACGTC AGCAGCTGGG ACATCTGGCA CTGCACTCAC TGCTCCAGTA 
ACAAATGGTA CATCAGCAGC AGGGACATCC GGCACTGTGA CAAGCGGTAC AGCAACAGCA 
GGGACATCCG GCACTGGCTC AGCAGTCACT ACAGCAACTC AAGCAGCAAC TGGGACATCC 
AGCACTGCAC TCACTGCTTC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCAATG CTCCAGTGAC AAGCGCTTCA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGTGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAC TACTCCAGTG 
ACAAGCGCTA CATCAATAAC TGGGACATCC AGCACTGCAC TCACTGCTTC AGTGACAAGT 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCAATG CTCCAGTGAC AAGCGGTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC ACTGCTCCAG TGACAAGCGC TTCATCAATA 
GCTGGGACAT CCAGCACTGC ACTCAATGCT CCAGTGACAA GCGCTACATC AACAGCTGGG 
ACATCCAGCA CTGCACTCAC TGCTCCAGTG ACAAGCGCTA CATCAATAGC TGGGACATCC 
AGCACTGCAC TCAATGCTCC AGTGACAAGC GCTACATCAA CAGCTGGGAC ATCCAGCACT 
GCACTCACTG CTCCAGTGAC AAGCGCTACA TCAATAGCTG GGACATCCAG CACTGCACTC 
ACTGCTTCAG TGACAAGCGC TACATCAATA GCTGGGACAT CCAGCACTGC ACTCACTGCT 
TCAGTGACAA GTGCTACATC AACAGCTGGG ACATCCAGCA CTGCACTCAA TGCTCCAGTG 
ACAAGCGCTA TATCAATAGC TGGGACATCC AGCACTTCAC TCACTGCTTC AGTGACAAGC 
GCTACATCAA CAGCTGGGAC ATCCAGCACT GCACTCACTG CTCCAGTGAC AAGCGTTACA 
TCAACAGCTG GGACATCCGG CACTGCACTC AATGATCCAG TGACAAGCGC TACATCAACA 
GCTGGGACAT CCGGCACTGC ACTCACTGCT CCAGTGACAA ACGCTACATC AACAGCTGGG 
ACATCCGGCA CTGGTGTTCT GTATGAATAC GGGAACGCAA CTGACAGTCT AAGCACAAGA 
GATGATGATG GTTCCGTGTT GGTAAATCCT ACTATCAACT TCCCGTTTGG GGGAAACTCC 
TATTCTTCTT TTTACCTGAG CAACAACGGA TACCTGGATT TCAATGGACC CGTTATTGCC 
CCGTTTTATG CAGACGTTTA TAACGTAATA TATGGTGACA TTTACTACCG GCAGAGCGCT 
GATCCCAGCC TCCTGGCAAA CGCAACGCAA GACATCAACA CCTACTTTCC AGGTTTAGGG 
TTCTCTGCCC AGTGGGTGTT TATAGCGACA TGGGACAAGG TGCCACGGTA TCCAGGAACT 
GCCAGTCAGG TGAATACGTT CCAGGTTGTT CTCATCACAA ATGGGACCAT TTCATTTGTT 
CTTTTTAACT ATGCGGATAT TCAATGGACA AATAATGCAC AGGCTTATTG GAACAACGGA 
ACCCAAGTTT ATCATTTAAA CACAGTAGGA TCTGTTTTCA ATTGGACAAC ATCAAGCAAC 
GTTAACTATC CTGGGCGGTG GGCTTTCATG GTAGGCAAAG TACAACCTGT AGCTCTCACT 
ACTCCAGTGA CAAGCAGTAC GTCAGCAGCG GGGACATCCG GCACCGCACT GACCACTCCA 
GTGACAAGTG GTTCATCAAC AGTGGGGGCA TCGGGCACTG CACTCACTGC TCCAGTGACA 
AGCGGTACAC CAACAGCGGG GACATCTGGC ACTGCACTCA CTGCTCCAGT GACAAGCGCT 
ACATCAACAG CTGGGACATC CAGCACTGCA CTCACTGCTC CAGTGACAAA CGATACATCA 
ACAGCTGGGA CATCCGGCAC TGGTGTTCTG TATGAATACG GGAACGCAAC TGACAGTCCA 
AGCACAAGAA CTTATGATGG TTCCGTGTTG GTAGATCTTA CTGTCGCGTT CCCTCTTTTT 
GGAAACACCT ATTCTTCTTT ATACCTGTAC AACAGAGGAT ACCTGAATTT CAATGGACCC 
CTTATTGTCC CATTTTATGC AGCCATTTAT AACTTAATAT CTGGTGACAT TTACTATCGG 
CAGAGCACTG ATTCCAGCCT CCTGGCAAAC GCAACGCAAG ACATCAACAC CTACTTTCCA 
GGTTTAGGGT TCTCTGCCCA GTTGGTGTTT ATAGCGACAT GGGACAAGGT GCCACAGTAT 
CAAGGAAATG CCAGTCAGGT GAATACGTTC CAGGTTGTTC TCATCACAGA TGGGACCATT 
TCATTTGTTC TTTTTAACTA TGCAGATATT CAGTGGACAT CTAATACAGT GGCTGGGTTG 
GACAGCAGAA ATGCTGCAGG ATATTATATA TTAAATACAA GTGGATCTAC TTTCAATTGG 
ACAAGCTCAA GTAACACTAA CTATCCTGGG CGGTGGGCTT TCAGAGTAGA CAGATTACAA 
ATTGAAGTAC CCGCACTACA GCAACCAGCA ACTACAGAAA CCACTACTCC AATGACAAGC 
GGTACGTCAG CAGCGGGGAC ATCTGGCACC GGACTCATGA CAGCAGAGAA AAGAGCCACA 
TCCGGCACTG GTGTTCTGTA TGAATACGGG AACGCAACTG ACAGTCTAAG CGCAAGAGGT 
GATGGTTATG TGTCGGTAGA TCTTACTGTC AACTTCCCTC TTTTTGGAAA CTCCTATTCT 
TCTTTTTACC TGTACAACAG AGGATACCTG GATTTCAGTC GACCCTCTAT TGTCCCGTTT 
TATGCAGCCA TTTCTACCGC AATATCTGGT AACATTTACT ACCGGCAGAG CGCTGATCCC 
AGCCTCCTGG CAAACGCAAC GCAAGACATC AACACCTACT TTCCAGGTTT AGGGTTCTCT 
GCCCAGTGGG TGTTTATAGC GACATGGGAC AAGGTGCCAC AGTATCAAGG AAATGCCAGT 
CAGGTGAATA CGTTCCAGGT TGTTCTCATC ACAGATGGGA CCATTTCATT TGTTCTTTTT 
AACTATGCGG ATATTCAGTG GACATCTAAT GCAGAGGCTG CGTTGCTCAG TGGAAATGCT 
GCAGGATATT ATCAATTAAA TACAAGTGGA TCTTCTTTCA ATTGGACAAG CTCAAGTAAC 
ACTAACTATC CTGGGCGATG GGCTTTCAGA GTAGACAGAT TACAAATTGA AGTACCCGCA 
CTACAGCAAC CAGCAACTAC AGAAACCACT ACTCCAATGA CAAGCGGTAC GTCAGCAGCG 
GGGACATCCG GCACCGCACT CACTGCTCCA GTGACAAGCG CTACATCAAC AGCGGGGACA 
TCCGGCACTG CACTCACTGC TCCAGTGACA AGCGCTAGAT CAACAGCTGG GACATCCAGC 
ACTGCACTCA CTGCTCCAGT GACAAGCGCT ATATCAACAG CTGGGACATC CAGCACTGCA 
CTCACTGCTT CAGTGACAAG CGCTACATCA ACAGCTGGGA CATCCGGCAC TGCAGTCAAT 
ACAGCAACTC TAGCAGCAGC TGGGACATCC GGCACTGCTC TCTCTACTCT AAACACAAGT 
AGCACGTCAT CAGCTGCGAC ATCCGGCACT GCTCTCTCTA CTCTCAAGAC AAGTACTACA 
CCAACAGATG TGATATCCAG CTCAGCACCC AGTATGGGCA CGGAGAGAGG AATGACATGG 
AATATTCAAG GAACATTTAC TACCTGCAAA ATATATGTGT ATATACTGTA CCTTTATATC 
TATGGTTTTC TTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC108645188 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: