znf335 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018097838.1	XP_017953327.1	zinc finger protein 335 isoform X1
XM_031894240.1	XP_031750100.1	zinc finger protein 335 isoform X1
XM_012952774.2	XP_012808228.1	zinc finger protein 335 isoform X4
XM_012952774.3	XP_012808228.2	zinc finger protein 335 isoform X2
XM_018097840.1	XP_017953329.1	zinc finger protein 335 isoform X6
XM_018097837.1	XP_017953326.1	zinc finger protein 335 isoform X1
XM_018097839.1	XP_017953328.1	zinc finger protein 335 isoform X2
XM_004918632.3	XP_004918689.1	zinc finger protein 335 isoform X3
XM_012952775.2	XP_012808229.1	zinc finger protein 335 isoform X5
XM_004918632.4	XP_004918689.2	zinc finger protein 335 isoform X1
XM_012952775.3	XP_012808229.2	zinc finger protein 335 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa17468	XM_004918632.3 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa37280	XM_012952774.2 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa37281	XM_012952775.2 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa47085	XM_018097837.1 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa47085	XM_018097838.1 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa47086	XM_018097839.1 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa47087	XM_018097840.1 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa50280	XM_031894240.1 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50280	XM_004918632.4 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50281	XM_012952774.3 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50282	XM_012952775.3 Latest version!	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

znf335 ( XM_004918632.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918689.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa17468
Clone ID Related Accession (Same CDS sequence)	XM_004918632.3
Accession Version	XM_004918632.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGAAGATA CAGCTTATAT TCAAGAGATC 
ACAACAGCTG ATGGACAGAC CCTGCAGCAG CTGGTCACAG CAGATAACCA GGTACAATAC 
ATTATCTCTC AGGAAGATAT CCCTCACCTC ATTCCCCAGG AATATGTTGT GCTACCAGAT 
GGACATCATA TCCAGGTCCA AGATGGTCAG ATAACACACA TTCAGTATGA ATCAGGCACC 
CCATTCCTGC AAGACCCCCA GATCCACTTT GTCCCCATGT CCCCTTCTCA GCAGCTCCTC 
ACCCAAGAAC AGCTCCAAGC TGCAGCGCAC TCTGCTGTCA CAGCCGTGGC AGATGCAGCG 
ATGGCCCAGA CAGTGTTCAG TACAGAAACG GAGCAGCCTC TTCCGCAAGA GATTGAATAT 
GATATTATCA CACTGGGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918689.1
CDS	526..4809
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEAELTPAPSLEEEHIFLTQEESMASQEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_004918632.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918632.3

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGAAGATA CAGCTTATAT TCAAGAGATC 
ACAACAGCTG ATGGACAGAC CCTGCAGCAG CTGGTCACAG CAGATAACCA GGTACAATAC 
ATTATCTCTC AGGAAGATAT CCCTCACCTC ATTCCCCAGG AATATGTTGT GCTACCAGAT 
GGACATCATA TCCAGGTCCA AGATGGTCAG ATAACACACA TTCAGTATGA ATCAGGCACC 
CCATTCCTGC AAGACCCCCA GATCCACTTT GTCCCCATGT CCCCTTCTCA GCAGCTCCTC 
ACCCAAGAAC AGCTCCAAGC TGCAGCGCAC TCTGCTGTCA CAGCCGTGGC AGATGCAGCG 
ATGGCCCAGA CAGTGTTCAG TACAGAAACG GAGCAGCCTC TTCCGCAAGA GATTGAATAT 
GATATTATCA CACTGGGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_012952774.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808228.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa37280
Clone ID Related Accession (Same CDS sequence)	XM_012952774.2
Accession Version	XM_012952774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGA GCTAACACCG GCCCCATCCC TGGAAGAGGA GCATATTTTC 
CTGACCCAAG AGGAATCCAT GGCCAGCCAG GAAGATACAG CTTATATTCA AGAGATCACA 
ACAGCTGATG GACAGACCCT GCAGCAGCTG GTCACAGCAG ATAACCAGGT ACAATACATT 
ATCTCTCAGG AAGATATCCC TCACCTCATT CCCCAGGAAT ATGTTGTGCT ACCAGATGGA 
CATCATATCC AGGTCCAAGA TGGTCAGATA ACACACATTC AGTATGAATC AGGCACCCCA 
TTCCTGCAAG ACCCCCAGAT CCACTTTGTC CCCATGTCCC CTTCTCAGCA GCTCCTCACC 
CAAGAACAGC TCCAAGCTGC AGCGCACTCT GCTGTCACAG CCGTGGCAGA TGCAGCGATG 
GCCCAGACAG TGTTCAGTAC AGAAACGGAG CAGCCTCTTC CGCAAGAGAT TGAATATGAT 
ATTATCACAC TGGGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808228.1
CDS	526..4806
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEELTPAPSLEEEHIFLTQEESMASQEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_012952774.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952774.2

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGA GCTAACACCG GCCCCATCCC TGGAAGAGGA GCATATTTTC 
CTGACCCAAG AGGAATCCAT GGCCAGCCAG GAAGATACAG CTTATATTCA AGAGATCACA 
ACAGCTGATG GACAGACCCT GCAGCAGCTG GTCACAGCAG ATAACCAGGT ACAATACATT 
ATCTCTCAGG AAGATATCCC TCACCTCATT CCCCAGGAAT ATGTTGTGCT ACCAGATGGA 
CATCATATCC AGGTCCAAGA TGGTCAGATA ACACACATTC AGTATGAATC AGGCACCCCA 
TTCCTGCAAG ACCCCCAGAT CCACTTTGTC CCCATGTCCC CTTCTCAGCA GCTCCTCACC 
CAAGAACAGC TCCAAGCTGC AGCGCACTCT GCTGTCACAG CCGTGGCAGA TGCAGCGATG 
GCCCAGACAG TGTTCAGTAC AGAAACGGAG CAGCCTCTTC CGCAAGAGAT TGAATATGAT 
ATTATCACAC TGGGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_012952775.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808229.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X6, mRNA.

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CloneID	OXa37281
Clone ID Related Accession (Same CDS sequence)	XM_012952775.2
Accession Version	XM_012952775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGCTGT CCCTCAATGT GTGGCCTCTT 
TCAGAATCAC CTGTCTGTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808229.1
CDS	526..4149
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRLSLNVWPLSESPVCA

Target ORF information:

RefSeq Version	XM_012952775.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952775.2

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGCTGT CCCTCAATGT GTGGCCTCTT 
TCAGAATCAC CTGTCTGTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_018097838.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953327.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa47085
Clone ID Related Accession (Same CDS sequence)	XM_018097838.1 , XM_018097837.1
Accession Version	XM_018097838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGTTTGCA GGACCGGAAT AATTTTGGAA 
GATACAGCTT ATATTCAAGA GATCACAACA GCTGATGGAC AGACCCTGCA GCAGCTGGTC 
ACAGCAGATA ACCAGGTACA ATACATTATC TCTCAGGAAG ATATCCCTCA CCTCATTCCC 
CAGGAATATG TTGTGCTACC AGATGGACAT CATATCCAGG TCCAAGATGG TCAGATAACA 
CACATTCAGT ATGAATCAGG CACCCCATTC CTGCAAGACC CCCAGATCCA CTTTGTCCCC 
ATGTCCCCTT CTCAGCAGCT CCTCACCCAA GAACAGCTCC AAGCTGCAGC GCACTCTGCT 
GTCACAGCCG TGGCAGATGC AGCGATGGCC CAGACAGTGT TCAGTACAGA AACGGAGCAG 
CCTCTTCCGC AAGAGATTGA ATATGATATT ATCACACTGG GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953327.1
CDS	182..4489
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEAELTPAPSLEEEHIFLTQEESMASQVCRTGIILEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_018097838.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097838.1

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGTTTGCA GGACCGGAAT AATTTTGGAA 
GATACAGCTT ATATTCAAGA GATCACAACA GCTGATGGAC AGACCCTGCA GCAGCTGGTC 
ACAGCAGATA ACCAGGTACA ATACATTATC TCTCAGGAAG ATATCCCTCA CCTCATTCCC 
CAGGAATATG TTGTGCTACC AGATGGACAT CATATCCAGG TCCAAGATGG TCAGATAACA 
CACATTCAGT ATGAATCAGG CACCCCATTC CTGCAAGACC CCCAGATCCA CTTTGTCCCC 
ATGTCCCCTT CTCAGCAGCT CCTCACCCAA GAACAGCTCC AAGCTGCAGC GCACTCTGCT 
GTCACAGCCG TGGCAGATGC AGCGATGGCC CAGACAGTGT TCAGTACAGA AACGGAGCAG 
CCTCTTCCGC AAGAGATTGA ATATGATATT ATCACACTGG GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_018097837.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953326.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47085
Clone ID Related Accession (Same CDS sequence)	XM_018097838.1 , XM_018097837.1
Accession Version	XM_018097837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGTTTGCA GGACCGGAAT AATTTTGGAA 
GATACAGCTT ATATTCAAGA GATCACAACA GCTGATGGAC AGACCCTGCA GCAGCTGGTC 
ACAGCAGATA ACCAGGTACA ATACATTATC TCTCAGGAAG ATATCCCTCA CCTCATTCCC 
CAGGAATATG TTGTGCTACC AGATGGACAT CATATCCAGG TCCAAGATGG TCAGATAACA 
CACATTCAGT ATGAATCAGG CACCCCATTC CTGCAAGACC CCCAGATCCA CTTTGTCCCC 
ATGTCCCCTT CTCAGCAGCT CCTCACCCAA GAACAGCTCC AAGCTGCAGC GCACTCTGCT 
GTCACAGCCG TGGCAGATGC AGCGATGGCC CAGACAGTGT TCAGTACAGA AACGGAGCAG 
CCTCTTCCGC AAGAGATTGA ATATGATATT ATCACACTGG GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953326.1
CDS	526..4833
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEAELTPAPSLEEEHIFLTQEESMASQVCRTGIILEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_018097837.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097837.1

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGTTTGCA GGACCGGAAT AATTTTGGAA 
GATACAGCTT ATATTCAAGA GATCACAACA GCTGATGGAC AGACCCTGCA GCAGCTGGTC 
ACAGCAGATA ACCAGGTACA ATACATTATC TCTCAGGAAG ATATCCCTCA CCTCATTCCC 
CAGGAATATG TTGTGCTACC AGATGGACAT CATATCCAGG TCCAAGATGG TCAGATAACA 
CACATTCAGT ATGAATCAGG CACCCCATTC CTGCAAGACC CCCAGATCCA CTTTGTCCCC 
ATGTCCCCTT CTCAGCAGCT CCTCACCCAA GAACAGCTCC AAGCTGCAGC GCACTCTGCT 
GTCACAGCCG TGGCAGATGC AGCGATGGCC CAGACAGTGT TCAGTACAGA AACGGAGCAG 
CCTCTTCCGC AAGAGATTGA ATATGATATT ATCACACTGG GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_018097839.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953328.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X3, mRNA.

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CloneID	OXa47086
Clone ID Related Accession (Same CDS sequence)	XM_018097839.1
Accession Version	XM_018097839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGA GCTAACACCG GCCCCATCCC TGGAAGAGGA GCATATTTTC 
CTGACCCAAG AGGAATCCAT GGCCAGCCAG GTTTGCAGGA CCGGAATAAT TTTGGAAGAT 
ACAGCTTATA TTCAAGAGAT CACAACAGCT GATGGACAGA CCCTGCAGCA GCTGGTCACA 
GCAGATAACC AGGTACAATA CATTATCTCT CAGGAAGATA TCCCTCACCT CATTCCCCAG 
GAATATGTTG TGCTACCAGA TGGACATCAT ATCCAGGTCC AAGATGGTCA GATAACACAC 
ATTCAGTATG AATCAGGCAC CCCATTCCTG CAAGACCCCC AGATCCACTT TGTCCCCATG 
TCCCCTTCTC AGCAGCTCCT CACCCAAGAA CAGCTCCAAG CTGCAGCGCA CTCTGCTGTC 
ACAGCCGTGG CAGATGCAGC GATGGCCCAG ACAGTGTTCA GTACAGAAAC GGAGCAGCCT 
CTTCCGCAAG AGATTGAATA TGATATTATC ACACTGGGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953328.1
CDS	526..4830
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEELTPAPSLEEEHIFLTQEESMASQVCRTGIILEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_018097839.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097839.1

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGA GCTAACACCG GCCCCATCCC TGGAAGAGGA GCATATTTTC 
CTGACCCAAG AGGAATCCAT GGCCAGCCAG GTTTGCAGGA CCGGAATAAT TTTGGAAGAT 
ACAGCTTATA TTCAAGAGAT CACAACAGCT GATGGACAGA CCCTGCAGCA GCTGGTCACA 
GCAGATAACC AGGTACAATA CATTATCTCT CAGGAAGATA TCCCTCACCT CATTCCCCAG 
GAATATGTTG TGCTACCAGA TGGACATCAT ATCCAGGTCC AAGATGGTCA GATAACACAC 
ATTCAGTATG AATCAGGCAC CCCATTCCTG CAAGACCCCC AGATCCACTT TGTCCCCATG 
TCCCCTTCTC AGCAGCTCCT CACCCAAGAA CAGCTCCAAG CTGCAGCGCA CTCTGCTGTC 
ACAGCCGTGG CAGATGCAGC GATGGCCCAG ACAGTGTTCA GTACAGAAAC GGAGCAGCCT 
CTTCCGCAAG AGATTGAATA TGATATTATC ACACTGGGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_018097840.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953329.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X7, mRNA.

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CloneID	OXa47087
Clone ID Related Accession (Same CDS sequence)	XM_018097840.1
Accession Version	XM_018097840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAAGG AACAGATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953329.1
CDS	526..4116
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNGLGWQGLDLRHTSGNLNLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEDEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRKEQM

Target ORF information:

RefSeq Version	XM_018097840.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097840.1

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATG GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGAAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GTGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGATG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAAGG AACAGATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_031894240.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750100.1 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa50280
Clone ID Related Accession (Same CDS sequence)	XM_031894240.1 , XM_004918632.4
Accession Version	XM_031894240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGAAGATA CAGCTTATAT TCAAGAGATC 
ACAACAGCTG ATGGACAGAC CCTGCAGCAG CTGGTCACAG CAGATAACCA GGTACAATAC 
ATTATCTCTC AGGAAGATAT CCCTCACCTC ATTCCCCAGG AATATGTTGT GCTACCAGAT 
GGACATCATA TCCAGGTCCA AGATGGTCAG ATAACACACA TTCAGTATGA ATCAGGCACC 
CCATTCCTGC AAGACCCCCA GATCCACTTT GTCCCCATGT CCCCTTCTCA GCAGCTCCTC 
ACCCAAGAAC AGCTCCAAGC TGCAGCGCAC TCTGCTGTCA CAGCCGTGGC AGATGCAGCG 
ATGGCCCAGA CAGTGTTCAG TACAGAAACG GAGCAGCCTC TTCCGCAAGA GATTGAATAT 
GATATTATCA CACTGGGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750100.1
CDS	176..4459
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNRLGWQGLDLRHTSGNLDLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEEEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEAELTPAPSLEEEHIFLTQEESMASQEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_031894240.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894240.1

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGAAGATA CAGCTTATAT TCAAGAGATC 
ACAACAGCTG ATGGACAGAC CCTGCAGCAG CTGGTCACAG CAGATAACCA GGTACAATAC 
ATTATCTCTC AGGAAGATAT CCCTCACCTC ATTCCCCAGG AATATGTTGT GCTACCAGAT 
GGACATCATA TCCAGGTCCA AGATGGTCAG ATAACACACA TTCAGTATGA ATCAGGCACC 
CCATTCCTGC AAGACCCCCA GATCCACTTT GTCCCCATGT CCCCTTCTCA GCAGCTCCTC 
ACCCAAGAAC AGCTCCAAGC TGCAGCGCAC TCTGCTGTCA CAGCCGTGGC AGATGCAGCG 
ATGGCCCAGA CAGTGTTCAG TACAGAAACG GAGCAGCCTC TTCCGCAAGA GATTGAATAT 
GATATTATCA CACTGGGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_004918632.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918689.2 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa50280
Clone ID Related Accession (Same CDS sequence)	XM_031894240.1 , XM_004918632.4
Accession Version	XM_004918632.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918632.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGAAGATA CAGCTTATAT TCAAGAGATC 
ACAACAGCTG ATGGACAGAC CCTGCAGCAG CTGGTCACAG CAGATAACCA GGTACAATAC 
ATTATCTCTC AGGAAGATAT CCCTCACCTC ATTCCCCAGG AATATGTTGT GCTACCAGAT 
GGACATCATA TCCAGGTCCA AGATGGTCAG ATAACACACA TTCAGTATGA ATCAGGCACC 
CCATTCCTGC AAGACCCCCA GATCCACTTT GTCCCCATGT CCCCTTCTCA GCAGCTCCTC 
ACCCAAGAAC AGCTCCAAGC TGCAGCGCAC TCTGCTGTCA CAGCCGTGGC AGATGCAGCG 
ATGGCCCAGA CAGTGTTCAG TACAGAAACG GAGCAGCCTC TTCCGCAAGA GATTGAATAT 
GATATTATCA CACTGGGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918689.2
CDS	520..4803
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNRLGWQGLDLRHTSGNLDLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEEEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEAELTPAPSLEEEHIFLTQEESMASQEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_004918632.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918632.4

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGC AGAGCTAACA CCGGCCCCAT CCCTGGAAGA GGAGCATATT 
TTCCTGACCC AAGAGGAATC CATGGCCAGC CAGGAAGATA CAGCTTATAT TCAAGAGATC 
ACAACAGCTG ATGGACAGAC CCTGCAGCAG CTGGTCACAG CAGATAACCA GGTACAATAC 
ATTATCTCTC AGGAAGATAT CCCTCACCTC ATTCCCCAGG AATATGTTGT GCTACCAGAT 
GGACATCATA TCCAGGTCCA AGATGGTCAG ATAACACACA TTCAGTATGA ATCAGGCACC 
CCATTCCTGC AAGACCCCCA GATCCACTTT GTCCCCATGT CCCCTTCTCA GCAGCTCCTC 
ACCCAAGAAC AGCTCCAAGC TGCAGCGCAC TCTGCTGTCA CAGCCGTGGC AGATGCAGCG 
ATGGCCCAGA CAGTGTTCAG TACAGAAACG GAGCAGCCTC TTCCGCAAGA GATTGAATAT 
GATATTATCA CACTGGGGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_012952774.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808228.2 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X3, mRNA.

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CloneID	OXa50281
Clone ID Related Accession (Same CDS sequence)	XM_012952774.3
Accession Version	XM_012952774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGA GCTAACACCG GCCCCATCCC TGGAAGAGGA GCATATTTTC 
CTGACCCAAG AGGAATCCAT GGCCAGCCAG GAAGATACAG CTTATATTCA AGAGATCACA 
ACAGCTGATG GACAGACCCT GCAGCAGCTG GTCACAGCAG ATAACCAGGT ACAATACATT 
ATCTCTCAGG AAGATATCCC TCACCTCATT CCCCAGGAAT ATGTTGTGCT ACCAGATGGA 
CATCATATCC AGGTCCAAGA TGGTCAGATA ACACACATTC AGTATGAATC AGGCACCCCA 
TTCCTGCAAG ACCCCCAGAT CCACTTTGTC CCCATGTCCC CTTCTCAGCA GCTCCTCACC 
CAAGAACAGC TCCAAGCTGC AGCGCACTCT GCTGTCACAG CCGTGGCAGA TGCAGCGATG 
GCCCAGACAG TGTTCAGTAC AGAAACGGAG CAGCCTCTTC CGCAAGAGAT TGAATATGAT 
ATTATCACAC TGGGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808228.2
CDS	520..4800
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNRLGWQGLDLRHTSGNLDLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEEEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRNHLSVHSDRRPHRCDQCSFACKNKRDLSRHSMIHTNQRPHSCPICGQRFTRKAHLKFHIQRLHRGKNNEPQENEELTPAPSLEEEHIFLTQEESMASQEDTAYIQEITTADGQTLQQLVTADNQVQYIISQEDIPHLIPQEYVVLPDGHHIQVQDGQITHIQYESGTPFLQDPQIHFVPMSPSQQLLTQEQLQAAAHSAVTAVADAAMAQTVFSTETEQPLPQEIEYDIITLGE

Target ORF information:

RefSeq Version	XM_012952774.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952774.3

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGAATC ACCTGTCTGT GCATAGCGAT 
CGCCGGCCGC ACCGCTGTGA TCAGTGCAGT TTTGCCTGCA AAAATAAGAG AGATCTGAGT 
AGGCATTCCA TGATACATAC CAACCAAAGG CCACACTCGT GTCCCATATG TGGGCAGAGG 
TTTACTAGGA AAGCTCACTT GAAGTTCCAC ATACAGAGGT TGCATCGAGG AAAGAACAAT 
GAGCCACAAG AGAATGAAGA GCTAACACCG GCCCCATCCC TGGAAGAGGA GCATATTTTC 
CTGACCCAAG AGGAATCCAT GGCCAGCCAG GAAGATACAG CTTATATTCA AGAGATCACA 
ACAGCTGATG GACAGACCCT GCAGCAGCTG GTCACAGCAG ATAACCAGGT ACAATACATT 
ATCTCTCAGG AAGATATCCC TCACCTCATT CCCCAGGAAT ATGTTGTGCT ACCAGATGGA 
CATCATATCC AGGTCCAAGA TGGTCAGATA ACACACATTC AGTATGAATC AGGCACCCCA 
TTCCTGCAAG ACCCCCAGAT CCACTTTGTC CCCATGTCCC CTTCTCAGCA GCTCCTCACC 
CAAGAACAGC TCCAAGCTGC AGCGCACTCT GCTGTCACAG CCGTGGCAGA TGCAGCGATG 
GCCCAGACAG TGTTCAGTAC AGAAACGGAG CAGCCTCTTC CGCAAGAGAT TGAATATGAT 
ATTATCACAC TGGGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf335 ( XM_012952775.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808229.2 Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X4, mRNA.

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CloneID	OXa50282
Clone ID Related Accession (Same CDS sequence)	XM_012952775.3
Accession Version	XM_012952775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 335 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGCTGT CCCTCAATGT GTGGCCTCTT 
TCAGAATCAC CTGTCTGTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808229.2
CDS	520..4143
Translation	MKQLSVQEEATKPLCGNVSSAEPLQVLLKPLNTTTQMATQELDCTDIKHSYSVQGIGSCKMDRPLTESDNIQSQDKTDTRDPDLKLDVRQDHNRLGWQGLDLRHTSGNLDLHKAPINEIRDSENTQQITEQSPGNTGIQELATSETPKGMKNPISSQEDLKQNPGDMGTLELAIIQTLHNIGHAMSSSEQTVKQSPVSMEITVNLPEDIDTNSQAVSQCTLYTHNTDVTQSQDIDSQTISHSADNMDSQELNIKAVSIAAQVLNMNSKTPNSMVQSRNPNYADTQDPNKRRTLESKCIIDTFSAQSSNNAVVQETGMIQSSDHIYIQDMVARHSSNIMASPDKDIIYHSYPEKISSAPIPSIPCSVSDASNVYQSLLSEGSSSILVQSDRPATAFSTGSVDGIINAPINPQIIQGCIVANPEGSNSEITQYLILQEQDSTSSVTVASVPVGERYQTHGSSCAAESMADAESLEGMVEVVFVQQYKCKMCKYKSTSKPTLLRHVRERHIQPTSIEEKRSHEGKVARSKEEEEEEEDDDDIVDAGAIDDPNDDSDYSPGEDTPNNNLESAHGILIKERPRRKPGRPRKIPRIDGPKLDIPLSSNDNQTDQGVTNSDLENKDPASLAISEEQSPPKRMRGRPPKHIRGKKYRRFRGSRFYRPPSKRLLRPFICRICGSSFLTHDDFTFHVTSHVGNDPQAFQCQQCSYQCRRWSSLKEHMYNHKGSKPYKCEECEYTSVYKKDVVRHSAVHREDRKKKSEKTSKPASYPCPVCGRIYTMQKRLTQHMKIHSAEKPHMCDKCGKAFKKRYTFKMHLLTHLQCVGKSRYKCEFCEYICGDRKQLLNHQLTHMNDKPFKCSQCKYHTYRQDFLLSHMATKHAGGKPFACDHCHFTTKHKKNLRLHVQSRHPLLYNEWSQRHPEETPSRRRPFFSMQQIEELKQLHEQSENQGPSPQEVVMDAAELSYQILQPTSQSPTEEQSHGALGAAAIIYEQEGEEEEYATRAALDLLLNMSTRREGTGGPLQVAVVKSGSGGEVQSSQSMAHEPHMAQVLTLHVEGNGVTSVQEAGYEDAGVEDTGYEGAVQQITIDNAFTTAEYSLINTENIPASLCSETSASPGSTLLGENIKDKILRKSQQAEHVSDHSAAPAAKKFCCKLCGTTFLGRSEMEIHKRAHVPSGGFKCPDCIFTAANWPEVRLSLNVWPLSESPVCA

Target ORF information:

RefSeq Version	XM_012952775.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 335 (znf335), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952775.3

ORF Insert Sequence:

ATGAAACAGT TATCTGTTCA GGAGGAAGCC ACCAAACCCT TGTGTGGGAA TGTCTCTAGT 
GCTGAGCCTT TGCAAGTTCT CCTTAAACCA CTAAATACCA CCACCCAAAT GGCTACGCAG 
GAACTGGACT GCACAGATAT CAAGCATTCA TATTCGGTAC AGGGTATCGG TAGCTGTAAG 
ATGGACAGAC CTTTGACTGA GTCGGACAAT ATACAGAGCC AAGACAAAAC AGACACTCGG 
GATCCTGATC TAAAACTGGA TGTAAGGCAA GATCACAATC GTTTAGGTTG GCAAGGGCTG 
GATCTGAGGC ATACTTCTGG TAACTTGGAT CTACACAAAG CTCCTATAAA TGAAATTAGA 
GATAGTGAAA ACACACAACA GATCACTGAA CAAAGCCCAG GCAATACTGG AATACAGGAA 
CTGGCTACAA GCGAAACACC AAAAGGCATG AAAAACCCCA TCAGTTCACA AGAAGACCTA 
AAACAGAACC CAGGTGACAT GGGCACTCTG GAACTGGCTA TAATACAAAC ACTTCATAAC 
ATAGGTCATG CCATGAGCTC CTCGGAACAA ACTGTAAAGC AGAGCCCAGT GAGCATGGAA 
ATAACTGTGA ATCTGCCAGA GGACATTGAT ACAAACAGCC AAGCTGTAAG CCAATGTACA 
CTTTACACAC ACAACACAGA TGTGACACAG AGCCAAGATA TAGACTCCCA AACGATCAGC 
CACAGTGCAG ATAACATGGA CTCTCAAGAG CTGAATATAA AAGCAGTTAG TATAGCTGCA 
CAAGTGCTAA ACATGAACTC CAAGACACCA AATAGCATGG TACAGAGCAG GAACCCAAAT 
TATGCTGATA CACAGGATCC AAACAAAAGA AGGACTTTAG AGAGCAAGTG CATCATAGAT 
ACTTTTTCAG CTCAGAGCTC AAATAACGCG GTTGTCCAGG AAACTGGCAT GATTCAGAGT 
TCGGATCACA TCTATATCCA AGATATGGTT GCAAGACACA GCTCGAATAT AATGGCTTCC 
CCGGACAAAG ACATCATATA CCATAGTTAT CCAGAAAAGA TCTCAAGTGC TCCGATACCA 
TCCATACCAT GTTCTGTGTC TGATGCAAGT AATGTTTATC AGTCACTTCT CTCTGAGGGC 
TCAAGCTCCA TTCTGGTTCA GTCAGACAGG CCAGCAACAG CCTTTTCCAC CGGTTCTGTA 
GATGGCATCA TTAATGCTCC TATCAACCCC CAGATAATTC AAGGCTGTAT TGTGGCAAAT 
CCTGAAGGCA GCAACTCTGA AATCACCCAA TACCTGATCC TTCAGGAGCA AGATAGTACC 
TCCTCTGTAA CTGTGGCTTC TGTACCTGTT GGGGAACGCT ACCAAACACA TGGCTCTTCT 
TGTGCTGCTG AAAGTATGGC TGATGCTGAA AGTTTGGAAG GGATGGTGGA AGTGGTCTTT 
GTGCAGCAGT ATAAGTGTAA GATGTGCAAA TACAAGAGCA CCTCCAAACC CACCTTGCTT 
CGTCATGTGA GAGAGCGCCA CATCCAGCCA ACTTCCATAG AAGAGAAGAG GAGCCACGAA 
GGGAAAGTCG CGCGGTCAAA AGAAGAAGAA GAAGAGGAGG AAGATGATGA TGATATTGTG 
GATGCTGGTG CAATTGATGA CCCAAATGAT GACAGCGATT ATAGCCCAGG AGAAGATACC 
CCAAACAATA ACTTAGAATC AGCTCATGGA ATACTAATTA AAGAGAGGCC ACGTAGGAAG 
CCAGGCCGCC CACGCAAGAT TCCGCGCATT GATGGTCCTA AACTGGACAT TCCACTGTCC 
AGCAACGACA ACCAGACAGA CCAGGGTGTT ACGAACTCTG ACCTTGAGAA CAAGGACCCT 
GCTTCCTTGG CCATATCTGA GGAACAGAGT CCACCCAAGA GGATGCGAGG CCGCCCTCCT 
AAGCATATTC GTGGGAAGAA ATACCGTAGA TTCAGAGGTA GTCGGTTTTA CAGACCCCCT 
AGTAAACGTC TTCTTAGACC CTTCATCTGC AGAATCTGCG GTTCCAGCTT CCTTACCCAT 
GATGATTTTA CTTTTCATGT GACATCACAT GTTGGTAATG ACCCACAAGC GTTTCAGTGT 
CAGCAGTGCA GTTATCAATG TCGACGCTGG TCTTCACTCA AGGAGCACAT GTATAATCAC 
AAGGGAAGCA AACCATATAA ATGCGAGGAG TGTGAGTACA CAAGTGTTTA CAAAAAGGAT 
GTGGTCAGGC ACTCAGCTGT GCATAGAGAA GATCGAAAGA AGAAATCCGA GAAGACTTCA 
AAACCAGCTT CCTACCCATG CCCTGTTTGT GGCCGTATAT ACACCATGCA AAAGAGGCTA 
ACCCAACATA TGAAGATACA CAGCGCTGAG AAACCTCACA TGTGTGATAA GTGTGGTAAA 
GCCTTCAAAA AGCGTTACAC CTTTAAGATG CACCTTCTGA CTCACTTGCA GTGTGTGGGG 
AAGAGCAGGT ACAAATGTGA GTTCTGTGAA TATATTTGTG GCGATCGAAA GCAACTCCTA 
AATCACCAAC TAACGCACAT GAACGACAAG CCATTCAAAT GTAGTCAATG CAAATACCAC 
ACCTACCGAC AGGACTTTCT GCTGTCCCAC ATGGCCACCA AACATGCAGG TGGGAAGCCC 
TTTGCTTGTG ACCACTGTCA CTTTACTACC AAGCACAAGA AGAATCTTCG GCTCCACGTG 
CAAAGCCGAC ATCCGCTCCT CTATAATGAG TGGTCTCAGC GGCACCCTGA GGAAACACCC 
AGTCGTCGAC GACCTTTTTT TTCTATGCAG CAGATTGAGG AGCTCAAGCA GCTGCATGAA 
CAGTCAGAAA ACCAGGGTCC CTCGCCTCAG GAAGTGGTGA TGGATGCAGC AGAACTCAGT 
TATCAAATAC TTCAGCCTAC ATCGCAGTCG CCAACAGAGG AGCAATCACA TGGTGCGCTT 
GGAGCTGCAG CCATTATATA TGAACAAGAA GGTGAAGAGG AAGAATATGC AACCAGGGCT 
GCTCTCGATC TGCTTCTCAA TATGAGCACT CGAAGGGAAG GCACAGGAGG CCCTCTGCAA 
GTGGCAGTAG TGAAATCTGG CTCCGGCGGG GAGGTTCAGA GCTCACAGAG CATGGCTCAT 
GAGCCCCACA TGGCTCAGGT CCTGACTTTG CATGTGGAAG GAAACGGAGT GACGAGCGTT 
CAAGAGGCTG GCTATGAGGA TGCAGGAGTT GAAGATACTG GATACGAAGG GGCTGTGCAG 
CAGATAACTA TCGACAATGC CTTCACAACT GCCGAATACA GCTTAATAAA CACAGAAAAT 
ATTCCAGCGT CACTGTGCAG TGAAACCAGT GCTTCTCCAG GATCAACACT GCTGGGGGAA 
AACATAAAGG ACAAAATCCT GCGCAAATCC CAGCAAGCTG AACATGTTTC AGATCATTCT 
GCTGCCCCTG CAGCCAAGAA ATTTTGCTGT AAGCTTTGTG GAACCACCTT TTTGGGTCGA 
TCTGAGATGG AGATTCACAA GAGAGCACAT GTCCCTAGCG GCGGATTTAA ATGCCCGGAC 
TGCATCTTTA CTGCTGCAAA CTGGCCTGAA GTGCGGCTGT CCCTCAATGT GTGGCCTCTT 
TCAGAATCAC CTGTCTGTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	znf335
Entrez Gene ID	100498615
Full Name	zinc finger protein 335
General protein information	Preferred Names zinc finger protein 335 Names zinc finger protein 335
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

znf335 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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