phlpp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	phlpp1
Entrez Gene ID	100497530
Full Name	PH domain and leucine rich repeat protein phosphatase 1
Synonyms	phlpp,plekhe1,scop
General protein information	Preferred Names PH domain and leucine rich repeat protein phosphatase 1 Names PH domain leucine-rich repeat-containing protein phosphatase 1 PH domain leucine-rich repeat protein phosphatase 1-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094955.1	XP_017950444.1	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X3
XM_002934390.5	XP_002934436.3	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X2
XM_004915308.4	XP_004915365.2	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X1
XM_004915308.3	XP_004915365.1	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X1
XM_002934390.4	XP_002934436.2	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHLPP1	XP_523952.2
Rattus norvegicus (Norway rat)	Phlpp1	NP_067689.1
Mus musculus (house mouse)	Phlpp1	NP_598582.3
Canis lupus familiaris (dog)	PHLPP1	XP_005615724.1
Bos taurus (cattle)	PHLPP1	XP_002707832.2
Xenopus tropicalis (tropical clawed frog)	LOC100497530	XP_004915365.1
Homo sapiens (human)	PHLPP1	NP_919431.2
	PHLPP1	XP_001094216.1
Gallus gallus (chicken)	PHLPP1	XP_418990.4

Gene Ontology

The following phlpp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phlpp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45126	XM_018094955.1 Latest version!	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa60628	XM_004915308.4 Latest version!	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa60629	XM_002934390.5 Latest version!	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa11757	XM_004915308.3 Latest version!	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa11758	XM_002934390.4 Latest version!	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phlpp1 ( XM_018094955.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950444.1 Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa45126
Clone ID Related Accession (Same CDS sequence)	XM_018094955.1
Accession Version	XM_018094955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCTGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAAGCTAA ATGAAATCAG AGGATTGGTA ATAGATGAGC CAGATTTTTT GCCTCACATC 
ACCCAGTTGG ACCTCCGAGA TAACAAGTTG ACTGATCTTG ATGTGACTGC CCTCAGCAAC 
TTAGAAGTCC TGCACTGTGA GAGGAACAGA CTAAGGTGTC TTAAAATCAG TGGCTTCTTC 
TTAAAAGCAC TTTATGCATC TTCTAATGAA CTCCTAAATC TTGATGTGTA TCCAAACCCT 
TGTTATTTAT CATACATGGA CATTTCTAGA AACTGTCTGC ATTCCCTTCC TGAGTGGTTC 
AGTGAATGCA GAAATTTAGA GGTACTGGAT GTGAGCCACA ATCACCTAAA TGAGCTTCCT 
GACCGGTTAT TCTGTACCAG TGCTTTGAGA AAGCTACTTT TAGGTTACAA CCAGCTGCAA 
AGGCTTCCAG AATTAATTGA GAACAACCAA CTTGAGGTAC TTGATGTGCA GCACAACCAG 
CTATTGGAGC TCCCTGCCAA CTTGCTGCTT AAATTAAACA GCCTAAGGTG CCTCAATGCC 
TCTGCCAACC ATTTAGAAAC CCTTCCTGCC ACATGTCTGT CCGAAGAGTC GCACAGTATG 
TTGCAGGAGC TGTATCTGAC TAATAATAAT CTCACTGACA AATGTGTGCC CTTATTAATG 
GGACATCCCC ACCTGAAGAT CCTGCACATG GCCTTCAATC ATCTACAGAC TTTCCCAGCA 
AGTAAAATGG CCAAACTGGA GGAACTAGAA GAAATAGATA TTAGCGGGAA CAAGCTGAAA 
GCCATGCCAA CTACCATAAT GAACTGCAGG CGCATGCACA CAGTCATTGC TCACTCCAAC 
TGCCTGGAAG TCTTCCCTGA AGTTATGCAG CTATCCGAGA TTAAGTGTGT GGACTTAAGC 
TGCAATGAAC TTACTGAAAT CACGTTGCCT GAAAACCTAC CTCCCAGACT GCAGGAACTG 
GACCTGACTG GTAATGCCCG CTTGCTCCTT GACCACAAAA CCCTGGAGCT TCTGAACAGC 
AACATTCGGT TCTTTAAGGT GGACCAGCCA TCCTGTGGGT TCTCTGGAAG TGAAGCATCA 
GGAGTGCCAG CTGTGTGGAG CCATGGATAT GCAGAGGCAT CAGGTGTTAA GAGCAAGCTT 
TGTGTAGCAT CCCTTTTGGT CAACAATTTC TGTGATAACC GTGAAGCTGT GTATGGTGTG 
TTTGATGGTG AGCGGAATGT CGAGGTACCC AGCCTGCTGC AGTGCACAAT GAGTGACATA 
CTAGCTGATG AACTGCAAAA AACAAAGAGC GAGGAGGAGT ACATGGGAAA TACCTTCCTG 
GTTATGCAGA GGAAACTGGG TACAGCTGGA CAAAAACTAG GGGGCTCTGC TGTCCTCTGC 
CACATAAAGC ATGATCCCAT GGACCCTGCT GGATGTTTCA CGCTCACCAC AGCCAATGTA 
GGCAAGTGCC AAACTGTTAT CTGCAGAGAT GGGAAGCCGC TTAACCTGTC CAAACACCTT 
ACAGTCAGCT GTGAAGAAGA ATTAAAGAGA ATAAAACAAC ACAAGGCCAT CATCACAGAG 
GATGGAAAAG TTAATGGAGT GACGGATTCA ACACGGATAC TGGGTTATAC ATTTCTTCAC 
CCAAGCGTCA CCCCCCGGCC GCATGTACAG TCTGTTACTC TAACACCACA GGACGAATTC 
TTCATCCTAG GAAGCAAGGG TTTGTGGGAT AGCCTTTCAA TGGAGGAAGC TGTTGAAACA 
GTGAGGAATG TACCAGATGC CCTGGCAGCT TCTAAGAAGT TGTGCAGCTT AGCCCAAAGC 
TATGGGTGCA GCGGTAGTTT GTGTGCAGTG GTGGTGCAGC TAAATGTCAC TGAAGACTGT 
ATTTGCTGCT GTGAGCTAAA TGGCGGCATG CCACCTCCCA GCCCTGGAAT TTTTACCCCA 
TCTGTCAACG TGGTTATCAA GGACCGGGCC CCTGACAGCC TTGGCATGCC ATCTTCCAGT 
AGTGGCTTAG CCTCAGAAAT AAGCAGTGAG ATATCCACTT CAGAGATGAG TAGTGAAGTT 
GGGTCGACTG CTTCTGATGA ACCCCCTGCA GTTTCTCTTC ATGAAAATGG AGTGGGTCCT 
GTTGGTTGTC CAACTGAGCA GCGTTGTACT CTACATCCAG TCTGTCTTTC AAGTTCCTTC 
CAGAGACAGC TCTCTAGTGC AACGTTCTCT AGTGCCTTCT CTGACAATGG ACTGGACAGT 
GAAGATGAGG AGCCAATAGA AGGTGTCTTC TCTAATGGGA GCCGAGTGGA GGTAGAAGTG 
GATATCCATT GTTCTCGAAC AAAGTCTGGA TGCCAGACTG TACCTGCAGT ACTTACAGAG 
TCTACCGACG AGGGTATTGT CATCAATGGC AATGAGGATG ACATGGGAGG TCCTGGTAAG 
AGACAAGACT TTTCCTCAAA TACTCTCGGT CGACAGAGAA GAGGCAATGG TTCAGTTGCA 
CCCTTAGAAA AGAGCCACAA CCTTATTGAA ATTGCAGCAG ATGCTCCTCT TAGAAAGCAA 
GGGGGCTATT TTGCAGCCCC TGCCCAGCCT GATCCTGATG ACCAATTCAT TATACCTCCT 
GAACTGGAGG AAGAAGTTAA GGAGATTATG AAACAACATC AGGAGCAGCA ACAACAACAA 
CAACAACAAC AGCAACAACA GCAGCAGCTG CAGCAGCAGC ACCGGCAGTA CCAAATGGAC 
CAATTGCCAG ACTACTATGA TACCCCACTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950444.1
CDS	711..5063
Translation	MLAHSPVRAAKVRVVETGAPRSMEQGAAEEPAAGPGTEEKKRPPVSAGNSTTGTLLLPPATNGSTSANSVLLLRRRRRRLKRNVSAIHSPSPLSSCPPGPASRSLERRAPLASRSRQWVPLQAQDRQCVRSELRRGCIHVYERHASPCLRPLICTLDTQSNQVATRLLRTGGSSGVLRILGPETSEQGSYFVAGVKGLGHCWEPGDEAPHQPASDSDSLSPTVCQGLELFHCGAASSSSSSSSSSVEELGMPTIPRAVTVPARPSREAAQCPASLYVQLHGESCRRLEAQEKPLQIQNDYLFQLGFRDLCRVQEEGMEPEIGCLIRFYAGKSNCGGFSERTQLSGTYNVRKGKMQLPVNRWARRQVILCGTCLIVSSVKESQTGKMHILPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTDYLRWLRQASKIASQRTSSVDLSCCSLEYLPENLFYSQDITHLNLKQNILRLSPGPAAEGTLDDLQRFTKLRSLNLSNNNLGSFPLAVCSIPTLTELNLSCNALRAIPSQVGDMNKLNEIRGLVIDEPDFLPHITQLDLRDNKLTDLDVTALSNLEVLHCERNRLRCLKISGFFLKALYASSNELLNLDVYPNPCYLSYMDISRNCLHSLPEWFSECRNLEVLDVSHNHLNELPDRLFCTSALRKLLLGYNQLQRLPELIENNQLEVLDVQHNQLLELPANLLLKLNSLRCLNASANHLETLPATCLSEESHSMLQELYLTNNNLTDKCVPLLMGHPHLKILHMAFNHLQTFPASKMAKLEELEEIDISGNKLKAMPTTIMNCRRMHTVIAHSNCLEVFPEVMQLSEIKCVDLSCNELTEITLPENLPPRLQELDLTGNARLLLDHKTLELLNSNIRFFKVDQPSCGFSGSEASGVPAVWSHGYAEASGVKSKLCVASLLVNNFCDNREAVYGVFDGERNVEVPSLLQCTMSDILADELQKTKSEEEYMGNTFLVMQRKLGTAGQKLGGSAVLCHIKHDPMDPAGCFTLTTANVGKCQTVICRDGKPLNLSKHLTVSCEEELKRIKQHKAIITEDGKVNGVTDSTRILGYTFLHPSVTPRPHVQSVTLTPQDEFFILGSKGLWDSLSMEEAVETVRNVPDALAASKKLCSLAQSYGCSGSLCAVVVQLNVTEDCICCCELNGGMPPPSPGIFTPSVNVVIKDRAPDSLGMPSSSSGLASEISSEISTSEMSSEVGSTASDEPPAVSLHENGVGPVGCPTEQRCTLHPVCLSSSFQRQLSSATFSSAFSDNGLDSEDEEPIEGVFSNGSRVEVEVDIHCSRTKSGCQTVPAVLTESTDEGIVINGNEDDMGGPGKRQDFSSNTLGRQRRGNGSVAPLEKSHNLIEIAADAPLRKQGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKQHQEQQQQQQQQQQQQQQLQQQHRQYQMDQLPDYYDTPL

Target ORF information:

RefSeq Version	XM_018094955.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094955.1

ORF Insert Sequence:

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCTGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAAGCTAA ATGAAATCAG AGGATTGGTA ATAGATGAGC CAGATTTTTT GCCTCACATC 
ACCCAGTTGG ACCTCCGAGA TAACAAGTTG ACTGATCTTG ATGTGACTGC CCTCAGCAAC 
TTAGAAGTCC TGCACTGTGA GAGGAACAGA CTAAGGTGTC TTAAAATCAG TGGCTTCTTC 
TTAAAAGCAC TTTATGCATC TTCTAATGAA CTCCTAAATC TTGATGTGTA TCCAAACCCT 
TGTTATTTAT CATACATGGA CATTTCTAGA AACTGTCTGC ATTCCCTTCC TGAGTGGTTC 
AGTGAATGCA GAAATTTAGA GGTACTGGAT GTGAGCCACA ATCACCTAAA TGAGCTTCCT 
GACCGGTTAT TCTGTACCAG TGCTTTGAGA AAGCTACTTT TAGGTTACAA CCAGCTGCAA 
AGGCTTCCAG AATTAATTGA GAACAACCAA CTTGAGGTAC TTGATGTGCA GCACAACCAG 
CTATTGGAGC TCCCTGCCAA CTTGCTGCTT AAATTAAACA GCCTAAGGTG CCTCAATGCC 
TCTGCCAACC ATTTAGAAAC CCTTCCTGCC ACATGTCTGT CCGAAGAGTC GCACAGTATG 
TTGCAGGAGC TGTATCTGAC TAATAATAAT CTCACTGACA AATGTGTGCC CTTATTAATG 
GGACATCCCC ACCTGAAGAT CCTGCACATG GCCTTCAATC ATCTACAGAC TTTCCCAGCA 
AGTAAAATGG CCAAACTGGA GGAACTAGAA GAAATAGATA TTAGCGGGAA CAAGCTGAAA 
GCCATGCCAA CTACCATAAT GAACTGCAGG CGCATGCACA CAGTCATTGC TCACTCCAAC 
TGCCTGGAAG TCTTCCCTGA AGTTATGCAG CTATCCGAGA TTAAGTGTGT GGACTTAAGC 
TGCAATGAAC TTACTGAAAT CACGTTGCCT GAAAACCTAC CTCCCAGACT GCAGGAACTG 
GACCTGACTG GTAATGCCCG CTTGCTCCTT GACCACAAAA CCCTGGAGCT TCTGAACAGC 
AACATTCGGT TCTTTAAGGT GGACCAGCCA TCCTGTGGGT TCTCTGGAAG TGAAGCATCA 
GGAGTGCCAG CTGTGTGGAG CCATGGATAT GCAGAGGCAT CAGGTGTTAA GAGCAAGCTT 
TGTGTAGCAT CCCTTTTGGT CAACAATTTC TGTGATAACC GTGAAGCTGT GTATGGTGTG 
TTTGATGGTG AGCGGAATGT CGAGGTACCC AGCCTGCTGC AGTGCACAAT GAGTGACATA 
CTAGCTGATG AACTGCAAAA AACAAAGAGC GAGGAGGAGT ACATGGGAAA TACCTTCCTG 
GTTATGCAGA GGAAACTGGG TACAGCTGGA CAAAAACTAG GGGGCTCTGC TGTCCTCTGC 
CACATAAAGC ATGATCCCAT GGACCCTGCT GGATGTTTCA CGCTCACCAC AGCCAATGTA 
GGCAAGTGCC AAACTGTTAT CTGCAGAGAT GGGAAGCCGC TTAACCTGTC CAAACACCTT 
ACAGTCAGCT GTGAAGAAGA ATTAAAGAGA ATAAAACAAC ACAAGGCCAT CATCACAGAG 
GATGGAAAAG TTAATGGAGT GACGGATTCA ACACGGATAC TGGGTTATAC ATTTCTTCAC 
CCAAGCGTCA CCCCCCGGCC GCATGTACAG TCTGTTACTC TAACACCACA GGACGAATTC 
TTCATCCTAG GAAGCAAGGG TTTGTGGGAT AGCCTTTCAA TGGAGGAAGC TGTTGAAACA 
GTGAGGAATG TACCAGATGC CCTGGCAGCT TCTAAGAAGT TGTGCAGCTT AGCCCAAAGC 
TATGGGTGCA GCGGTAGTTT GTGTGCAGTG GTGGTGCAGC TAAATGTCAC TGAAGACTGT 
ATTTGCTGCT GTGAGCTAAA TGGCGGCATG CCACCTCCCA GCCCTGGAAT TTTTACCCCA 
TCTGTCAACG TGGTTATCAA GGACCGGGCC CCTGACAGCC TTGGCATGCC ATCTTCCAGT 
AGTGGCTTAG CCTCAGAAAT AAGCAGTGAG ATATCCACTT CAGAGATGAG TAGTGAAGTT 
GGGTCGACTG CTTCTGATGA ACCCCCTGCA GTTTCTCTTC ATGAAAATGG AGTGGGTCCT 
GTTGGTTGTC CAACTGAGCA GCGTTGTACT CTACATCCAG TCTGTCTTTC AAGTTCCTTC 
CAGAGACAGC TCTCTAGTGC AACGTTCTCT AGTGCCTTCT CTGACAATGG ACTGGACAGT 
GAAGATGAGG AGCCAATAGA AGGTGTCTTC TCTAATGGGA GCCGAGTGGA GGTAGAAGTG 
GATATCCATT GTTCTCGAAC AAAGTCTGGA TGCCAGACTG TACCTGCAGT ACTTACAGAG 
TCTACCGACG AGGGTATTGT CATCAATGGC AATGAGGATG ACATGGGAGG TCCTGGTAAG 
AGACAAGACT TTTCCTCAAA TACTCTCGGT CGACAGAGAA GAGGCAATGG TTCAGTTGCA 
CCCTTAGAAA AGAGCCACAA CCTTATTGAA ATTGCAGCAG ATGCTCCTCT TAGAAAGCAA 
GGGGGCTATT TTGCAGCCCC TGCCCAGCCT GATCCTGATG ACCAATTCAT TATACCTCCT 
GAACTGGAGG AAGAAGTTAA GGAGATTATG AAACAACATC AGGAGCAGCA ACAACAACAA 
CAACAACAAC AGCAACAACA GCAGCAGCTG CAGCAGCAGC ACCGGCAGTA CCAAATGGAC 
CAATTGCCAG ACTACTATGA TACCCCACTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phlpp1 ( XM_004915308.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915365.2 Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa60628
Clone ID Related Accession (Same CDS sequence)	XM_004915308.4
Accession Version	XM_004915308.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915308.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCAGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAAAGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAG CAACATTCGG 
TTCTTAAAGG TGGACCAGCC ATCCTGTGGG TTCTCTGGAA GTGAAGCATC AGGAGTGCCA 
GCTGTGTGGA GCCATGGATA TGCAGAGGCA TCAGGTGTTA AGAGCAAGCT TTGTGTAGCA 
TCCCTTTTGG TCAACAATTT CTGTGATAAC CGTGAAGCTG TGTATGGTGT GTTTGATGGT 
GAGCGGAATG TCGAGGTACC CAGCCTGCTG CAGTGCACAA TGAGTGACAT ACTAGCTGAT 
GAACTGCAAA AAACAAAGAG CGAGGAGGAG TACATGGGAA ATACCTTCCT GGTTATGCAG 
AGGAAACTGG GTACAGCTGG ACAAAAACTA GGGGGCTCTG CTGTCCTCTG CCACATAAAG 
CATGATCCCA TGGACCCTGC TGGATGTTTC ACGCTCACCA CAGCCAATGT AGGCAAGTGC 
CAAACTGTTA TCTGCAGAGA TGGGAAGCCG CTTAACCTGT CCAAACACCT TACAGTCAGC 
TGTGAAGAAG AATTAAAGAG AATAAAACAA CACAAGGCCA TCATCACAGA GGATGGAAAA 
GTTAATGGAG TGACGGATTC AACACGGATA CTGGGTTATA CATTTCTTCA CCCAAGCGTC 
ACCCCCCGGC CGCATGTACA GTCTGTTACT CTAACACCAC AGGACGAATT CTTCATCCTA 
GGAAGCAAGG GTTTGTGGGA TAGCCTTTCA ATGGAAGAAG CTGTTGAAAC AGTGAGGAAT 
GTACCAGATG CCCTGGCAGC TTCTAAGAAG TTGTGCAGCT TAGCCCAAAG CTATGGGTGC 
AGCGGTAGTT TGTGTGCAGT GGTAGTGCAG CTAAATGTCA CTGAAGACTG TATTTGCTGC 
TGTGAGCTAA ATGGCGGCAT GCCACCTCCC AGCCCTGGAA TTTTTACCCC ATCTGTCAAC 
GTGGTTATCA AGGACCGGGC CCCTGACAGC CTTGGCATGC CATCTTCCAG TAGTGGCTTA 
GCCTCAGAAA TAAGCAGTGA GATATCCACT TCAGAGATGA GTAGTGAAGT TGGGTCGACT 
GCTTCTGATG AACCCCCTGC AGTTTCTCTT CATGAAAATG GAGTGGGTCC TGTTGGTTGT 
CCAACTGAGC AGCGTTGTAC TCTACATCCA GTCTGTCTTT CAAGTTCCTT CCAGAGACAG 
CTCTCTAGTG CAACGTTCTC TAGTGCCTTC TCTGACAATG GACTGGACAG TGAAGATGAG 
GAGCCAATAG AAGGTGTCTT CTCTAATGGG AGCCGAGTGG AGGTAGAAGT GGATATCCAT 
TGTTCTCGAA CAAAGTCTGG ATGCCAGACT GTACCTGCAG TACTTACAGA GTCTACCGAC 
GAGGGTATTG TCATCAATGG CAATGAGGAT GACATGGGAG GTCCTGGTAA GAGACAAGAC 
TTTTCCTCTA ATACTCTCGG TCGACAGAGA AGAGGCAATG GTTCAGTTGC ACCCTTAGAA 
AAGAGCCACA ACCTTATTGA AATTGCAGCA GATGCTCCTC TTAGAAAGCA AGGGGGCTAT 
TTTGCAGCCC CTGCCCAGCC TGATCCTGAT GACCAATTCA TTATACCTCC TGAACTGGAG 
GAAGAAGTTA AGGAGATTAT GAAACAACAT CAGGAGCAGC AGCAACAACA ACAACAACAA 
CAGCAACAAC AGCAGCAGCT GCAGCAACAA CAACAACTGC AGCAGCAGCA CCGGCAGTAC 
CAAATGGACC AATTGCCAGA CTACTATGAT ACCCCACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915365.2
CDS	493..5094
Translation	MLAHSPVRAAKVRVVETGAPRSMEQGAAEEPAAGPGTEEKKRPPVSAGNSTTGTLLLPPATNGSTSANSVLLLRRRRRRLKRNVSAIHSPSPLSSCPPGPASRSLERRAPLASRSRQWVPLQAQDRQCVRSELRRGCIHVYERHASPCLRPLICTLDTQSNQVATRLLRTGGSSGVLRILGPETSEQGSYFVAGVKGLGHCWEPGDEAPHQPASDSDSLSPTVCQGLELFHCGAASSSSSSSSSSVEELGMPTIPRAVTVPARPSREAAQCPASLYVQLHGESCRRLEAQEKPLQIQNDYLFQLGFRDLCRVQEEGMEPEIGCLIRFYAGKSNCGGFSERTQLSGTYNVRKGKMQLPVNRWARRQVILCGTCLIVSSVKESQTGKMHILPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTDYLRWLRQASKIASQRTSSVDLSCCSLEYLPENLFYSQDITHLNLKQNILRLSPGPAAEGTLDDLQRFTKLRSLNLSNNNLGSFPLAVCSIPTLTELNLSCNALRAIPSQVGDMNNLQTFLLDGNYLQSLPAEMGNMHQLNYLGLSFNEFTDIPEVVEKMASMDRLCMPGNALDILELQTLRKMSQIKHVDLRLNEIRGLVIDEPDFLPHITQLDLRDNKLTDLDVTALSNLEVLHCERNRLRCLKISGFFLKALYASSNELLNLDVYPNPCYLSYMDISRNCLHSLPEWFSESRNLEVLDVSHNHLNELPDRLFCTSALRKLLLGYNQLQRLPELIENNQLEVLDVQHNQLLELPANLLLKLNSLRCLNASANHLETLPATCLSEESHSMLQELYLTNNNLTDKCVPLLMGHPHLKILHMAFNHLQTFPASKMAKLEELEEIDISGNKLKAMPTTIMNCRRMHTVIAHSNCLEVFPEVMQLSEIKCVDLSCNELTEITLPENLPPRLQELDLTGNARLLLDHKTLELLNSNIRFLKVDQPSCGFSGSEASGVPAVWSHGYAEASGVKSKLCVASLLVNNFCDNREAVYGVFDGERNVEVPSLLQCTMSDILADELQKTKSEEEYMGNTFLVMQRKLGTAGQKLGGSAVLCHIKHDPMDPAGCFTLTTANVGKCQTVICRDGKPLNLSKHLTVSCEEELKRIKQHKAIITEDGKVNGVTDSTRILGYTFLHPSVTPRPHVQSVTLTPQDEFFILGSKGLWDSLSMEEAVETVRNVPDALAASKKLCSLAQSYGCSGSLCAVVVQLNVTEDCICCCELNGGMPPPSPGIFTPSVNVVIKDRAPDSLGMPSSSSGLASEISSEISTSEMSSEVGSTASDEPPAVSLHENGVGPVGCPTEQRCTLHPVCLSSSFQRQLSSATFSSAFSDNGLDSEDEEPIEGVFSNGSRVEVEVDIHCSRTKSGCQTVPAVLTESTDEGIVINGNEDDMGGPGKRQDFSSNTLGRQRRGNGSVAPLEKSHNLIEIAADAPLRKQGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKQHQEQQQQQQQQQQQQQQLQQQQQLQQQHRQYQMDQLPDYYDTPL

Target ORF information:

RefSeq Version	XM_004915308.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915308.4

ORF Insert Sequence:

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCAGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAAAGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAG CAACATTCGG 
TTCTTAAAGG TGGACCAGCC ATCCTGTGGG TTCTCTGGAA GTGAAGCATC AGGAGTGCCA 
GCTGTGTGGA GCCATGGATA TGCAGAGGCA TCAGGTGTTA AGAGCAAGCT TTGTGTAGCA 
TCCCTTTTGG TCAACAATTT CTGTGATAAC CGTGAAGCTG TGTATGGTGT GTTTGATGGT 
GAGCGGAATG TCGAGGTACC CAGCCTGCTG CAGTGCACAA TGAGTGACAT ACTAGCTGAT 
GAACTGCAAA AAACAAAGAG CGAGGAGGAG TACATGGGAA ATACCTTCCT GGTTATGCAG 
AGGAAACTGG GTACAGCTGG ACAAAAACTA GGGGGCTCTG CTGTCCTCTG CCACATAAAG 
CATGATCCCA TGGACCCTGC TGGATGTTTC ACGCTCACCA CAGCCAATGT AGGCAAGTGC 
CAAACTGTTA TCTGCAGAGA TGGGAAGCCG CTTAACCTGT CCAAACACCT TACAGTCAGC 
TGTGAAGAAG AATTAAAGAG AATAAAACAA CACAAGGCCA TCATCACAGA GGATGGAAAA 
GTTAATGGAG TGACGGATTC AACACGGATA CTGGGTTATA CATTTCTTCA CCCAAGCGTC 
ACCCCCCGGC CGCATGTACA GTCTGTTACT CTAACACCAC AGGACGAATT CTTCATCCTA 
GGAAGCAAGG GTTTGTGGGA TAGCCTTTCA ATGGAAGAAG CTGTTGAAAC AGTGAGGAAT 
GTACCAGATG CCCTGGCAGC TTCTAAGAAG TTGTGCAGCT TAGCCCAAAG CTATGGGTGC 
AGCGGTAGTT TGTGTGCAGT GGTAGTGCAG CTAAATGTCA CTGAAGACTG TATTTGCTGC 
TGTGAGCTAA ATGGCGGCAT GCCACCTCCC AGCCCTGGAA TTTTTACCCC ATCTGTCAAC 
GTGGTTATCA AGGACCGGGC CCCTGACAGC CTTGGCATGC CATCTTCCAG TAGTGGCTTA 
GCCTCAGAAA TAAGCAGTGA GATATCCACT TCAGAGATGA GTAGTGAAGT TGGGTCGACT 
GCTTCTGATG AACCCCCTGC AGTTTCTCTT CATGAAAATG GAGTGGGTCC TGTTGGTTGT 
CCAACTGAGC AGCGTTGTAC TCTACATCCA GTCTGTCTTT CAAGTTCCTT CCAGAGACAG 
CTCTCTAGTG CAACGTTCTC TAGTGCCTTC TCTGACAATG GACTGGACAG TGAAGATGAG 
GAGCCAATAG AAGGTGTCTT CTCTAATGGG AGCCGAGTGG AGGTAGAAGT GGATATCCAT 
TGTTCTCGAA CAAAGTCTGG ATGCCAGACT GTACCTGCAG TACTTACAGA GTCTACCGAC 
GAGGGTATTG TCATCAATGG CAATGAGGAT GACATGGGAG GTCCTGGTAA GAGACAAGAC 
TTTTCCTCTA ATACTCTCGG TCGACAGAGA AGAGGCAATG GTTCAGTTGC ACCCTTAGAA 
AAGAGCCACA ACCTTATTGA AATTGCAGCA GATGCTCCTC TTAGAAAGCA AGGGGGCTAT 
TTTGCAGCCC CTGCCCAGCC TGATCCTGAT GACCAATTCA TTATACCTCC TGAACTGGAG 
GAAGAAGTTA AGGAGATTAT GAAACAACAT CAGGAGCAGC AGCAACAACA ACAACAACAA 
CAGCAACAAC AGCAGCAGCT GCAGCAACAA CAACAACTGC AGCAGCAGCA CCGGCAGTAC 
CAAATGGACC AATTGCCAGA CTACTATGAT ACCCCACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phlpp1 ( XM_002934390.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934436.3 Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X2, mRNA.

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CloneID	OXa60629
Clone ID Related Accession (Same CDS sequence)	XM_002934390.5
Accession Version	XM_002934390.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934390.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCAGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAAAGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAA CATTCGGTTC 
TTAAAGGTGG ACCAGCCATC CTGTGGGTTC TCTGGAAGTG AAGCATCAGG AGTGCCAGCT 
GTGTGGAGCC ATGGATATGC AGAGGCATCA GGTGTTAAGA GCAAGCTTTG TGTAGCATCC 
CTTTTGGTCA ACAATTTCTG TGATAACCGT GAAGCTGTGT ATGGTGTGTT TGATGGTGAG 
CGGAATGTCG AGGTACCCAG CCTGCTGCAG TGCACAATGA GTGACATACT AGCTGATGAA 
CTGCAAAAAA CAAAGAGCGA GGAGGAGTAC ATGGGAAATA CCTTCCTGGT TATGCAGAGG 
AAACTGGGTA CAGCTGGACA AAAACTAGGG GGCTCTGCTG TCCTCTGCCA CATAAAGCAT 
GATCCCATGG ACCCTGCTGG ATGTTTCACG CTCACCACAG CCAATGTAGG CAAGTGCCAA 
ACTGTTATCT GCAGAGATGG GAAGCCGCTT AACCTGTCCA AACACCTTAC AGTCAGCTGT 
GAAGAAGAAT TAAAGAGAAT AAAACAACAC AAGGCCATCA TCACAGAGGA TGGAAAAGTT 
AATGGAGTGA CGGATTCAAC ACGGATACTG GGTTATACAT TTCTTCACCC AAGCGTCACC 
CCCCGGCCGC ATGTACAGTC TGTTACTCTA ACACCACAGG ACGAATTCTT CATCCTAGGA 
AGCAAGGGTT TGTGGGATAG CCTTTCAATG GAAGAAGCTG TTGAAACAGT GAGGAATGTA 
CCAGATGCCC TGGCAGCTTC TAAGAAGTTG TGCAGCTTAG CCCAAAGCTA TGGGTGCAGC 
GGTAGTTTGT GTGCAGTGGT AGTGCAGCTA AATGTCACTG AAGACTGTAT TTGCTGCTGT 
GAGCTAAATG GCGGCATGCC ACCTCCCAGC CCTGGAATTT TTACCCCATC TGTCAACGTG 
GTTATCAAGG ACCGGGCCCC TGACAGCCTT GGCATGCCAT CTTCCAGTAG TGGCTTAGCC 
TCAGAAATAA GCAGTGAGAT ATCCACTTCA GAGATGAGTA GTGAAGTTGG GTCGACTGCT 
TCTGATGAAC CCCCTGCAGT TTCTCTTCAT GAAAATGGAG TGGGTCCTGT TGGTTGTCCA 
ACTGAGCAGC GTTGTACTCT ACATCCAGTC TGTCTTTCAA GTTCCTTCCA GAGACAGCTC 
TCTAGTGCAA CGTTCTCTAG TGCCTTCTCT GACAATGGAC TGGACAGTGA AGATGAGGAG 
CCAATAGAAG GTGTCTTCTC TAATGGGAGC CGAGTGGAGG TAGAAGTGGA TATCCATTGT 
TCTCGAACAA AGTCTGGATG CCAGACTGTA CCTGCAGTAC TTACAGAGTC TACCGACGAG 
GGTATTGTCA TCAATGGCAA TGAGGATGAC ATGGGAGGTC CTGGTAAGAG ACAAGACTTT 
TCCTCTAATA CTCTCGGTCG ACAGAGAAGA GGCAATGGTT CAGTTGCACC CTTAGAAAAG 
AGCCACAACC TTATTGAAAT TGCAGCAGAT GCTCCTCTTA GAAAGCAAGG GGGCTATTTT 
GCAGCCCCTG CCCAGCCTGA TCCTGATGAC CAATTCATTA TACCTCCTGA ACTGGAGGAA 
GAAGTTAAGG AGATTATGAA ACAACATCAG GAGCAGCAGC AACAACAACA ACAACAACAG 
CAACAACAGC AGCAGCTGCA GCAACAACAA CAACTGCAGC AGCAGCACCG GCAGTACCAA 
ATGGACCAAT TGCCAGACTA CTATGATACC CCACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934436.3
CDS	493..5091
Translation	MLAHSPVRAAKVRVVETGAPRSMEQGAAEEPAAGPGTEEKKRPPVSAGNSTTGTLLLPPATNGSTSANSVLLLRRRRRRLKRNVSAIHSPSPLSSCPPGPASRSLERRAPLASRSRQWVPLQAQDRQCVRSELRRGCIHVYERHASPCLRPLICTLDTQSNQVATRLLRTGGSSGVLRILGPETSEQGSYFVAGVKGLGHCWEPGDEAPHQPASDSDSLSPTVCQGLELFHCGAASSSSSSSSSSVEELGMPTIPRAVTVPARPSREAAQCPASLYVQLHGESCRRLEAQEKPLQIQNDYLFQLGFRDLCRVQEEGMEPEIGCLIRFYAGKSNCGGFSERTQLSGTYNVRKGKMQLPVNRWARRQVILCGTCLIVSSVKESQTGKMHILPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTDYLRWLRQASKIASQRTSSVDLSCCSLEYLPENLFYSQDITHLNLKQNILRLSPGPAAEGTLDDLQRFTKLRSLNLSNNNLGSFPLAVCSIPTLTELNLSCNALRAIPSQVGDMNNLQTFLLDGNYLQSLPAEMGNMHQLNYLGLSFNEFTDIPEVVEKMASMDRLCMPGNALDILELQTLRKMSQIKHVDLRLNEIRGLVIDEPDFLPHITQLDLRDNKLTDLDVTALSNLEVLHCERNRLRCLKISGFFLKALYASSNELLNLDVYPNPCYLSYMDISRNCLHSLPEWFSESRNLEVLDVSHNHLNELPDRLFCTSALRKLLLGYNQLQRLPELIENNQLEVLDVQHNQLLELPANLLLKLNSLRCLNASANHLETLPATCLSEESHSMLQELYLTNNNLTDKCVPLLMGHPHLKILHMAFNHLQTFPASKMAKLEELEEIDISGNKLKAMPTTIMNCRRMHTVIAHSNCLEVFPEVMQLSEIKCVDLSCNELTEITLPENLPPRLQELDLTGNARLLLDHKTLELLNNIRFLKVDQPSCGFSGSEASGVPAVWSHGYAEASGVKSKLCVASLLVNNFCDNREAVYGVFDGERNVEVPSLLQCTMSDILADELQKTKSEEEYMGNTFLVMQRKLGTAGQKLGGSAVLCHIKHDPMDPAGCFTLTTANVGKCQTVICRDGKPLNLSKHLTVSCEEELKRIKQHKAIITEDGKVNGVTDSTRILGYTFLHPSVTPRPHVQSVTLTPQDEFFILGSKGLWDSLSMEEAVETVRNVPDALAASKKLCSLAQSYGCSGSLCAVVVQLNVTEDCICCCELNGGMPPPSPGIFTPSVNVVIKDRAPDSLGMPSSSSGLASEISSEISTSEMSSEVGSTASDEPPAVSLHENGVGPVGCPTEQRCTLHPVCLSSSFQRQLSSATFSSAFSDNGLDSEDEEPIEGVFSNGSRVEVEVDIHCSRTKSGCQTVPAVLTESTDEGIVINGNEDDMGGPGKRQDFSSNTLGRQRRGNGSVAPLEKSHNLIEIAADAPLRKQGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKQHQEQQQQQQQQQQQQQQLQQQQQLQQQHRQYQMDQLPDYYDTPL

Target ORF information:

RefSeq Version	XM_002934390.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934390.5

ORF Insert Sequence:

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCAGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAAAGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAA CATTCGGTTC 
TTAAAGGTGG ACCAGCCATC CTGTGGGTTC TCTGGAAGTG AAGCATCAGG AGTGCCAGCT 
GTGTGGAGCC ATGGATATGC AGAGGCATCA GGTGTTAAGA GCAAGCTTTG TGTAGCATCC 
CTTTTGGTCA ACAATTTCTG TGATAACCGT GAAGCTGTGT ATGGTGTGTT TGATGGTGAG 
CGGAATGTCG AGGTACCCAG CCTGCTGCAG TGCACAATGA GTGACATACT AGCTGATGAA 
CTGCAAAAAA CAAAGAGCGA GGAGGAGTAC ATGGGAAATA CCTTCCTGGT TATGCAGAGG 
AAACTGGGTA CAGCTGGACA AAAACTAGGG GGCTCTGCTG TCCTCTGCCA CATAAAGCAT 
GATCCCATGG ACCCTGCTGG ATGTTTCACG CTCACCACAG CCAATGTAGG CAAGTGCCAA 
ACTGTTATCT GCAGAGATGG GAAGCCGCTT AACCTGTCCA AACACCTTAC AGTCAGCTGT 
GAAGAAGAAT TAAAGAGAAT AAAACAACAC AAGGCCATCA TCACAGAGGA TGGAAAAGTT 
AATGGAGTGA CGGATTCAAC ACGGATACTG GGTTATACAT TTCTTCACCC AAGCGTCACC 
CCCCGGCCGC ATGTACAGTC TGTTACTCTA ACACCACAGG ACGAATTCTT CATCCTAGGA 
AGCAAGGGTT TGTGGGATAG CCTTTCAATG GAAGAAGCTG TTGAAACAGT GAGGAATGTA 
CCAGATGCCC TGGCAGCTTC TAAGAAGTTG TGCAGCTTAG CCCAAAGCTA TGGGTGCAGC 
GGTAGTTTGT GTGCAGTGGT AGTGCAGCTA AATGTCACTG AAGACTGTAT TTGCTGCTGT 
GAGCTAAATG GCGGCATGCC ACCTCCCAGC CCTGGAATTT TTACCCCATC TGTCAACGTG 
GTTATCAAGG ACCGGGCCCC TGACAGCCTT GGCATGCCAT CTTCCAGTAG TGGCTTAGCC 
TCAGAAATAA GCAGTGAGAT ATCCACTTCA GAGATGAGTA GTGAAGTTGG GTCGACTGCT 
TCTGATGAAC CCCCTGCAGT TTCTCTTCAT GAAAATGGAG TGGGTCCTGT TGGTTGTCCA 
ACTGAGCAGC GTTGTACTCT ACATCCAGTC TGTCTTTCAA GTTCCTTCCA GAGACAGCTC 
TCTAGTGCAA CGTTCTCTAG TGCCTTCTCT GACAATGGAC TGGACAGTGA AGATGAGGAG 
CCAATAGAAG GTGTCTTCTC TAATGGGAGC CGAGTGGAGG TAGAAGTGGA TATCCATTGT 
TCTCGAACAA AGTCTGGATG CCAGACTGTA CCTGCAGTAC TTACAGAGTC TACCGACGAG 
GGTATTGTCA TCAATGGCAA TGAGGATGAC ATGGGAGGTC CTGGTAAGAG ACAAGACTTT 
TCCTCTAATA CTCTCGGTCG ACAGAGAAGA GGCAATGGTT CAGTTGCACC CTTAGAAAAG 
AGCCACAACC TTATTGAAAT TGCAGCAGAT GCTCCTCTTA GAAAGCAAGG GGGCTATTTT 
GCAGCCCCTG CCCAGCCTGA TCCTGATGAC CAATTCATTA TACCTCCTGA ACTGGAGGAA 
GAAGTTAAGG AGATTATGAA ACAACATCAG GAGCAGCAGC AACAACAACA ACAACAACAG 
CAACAACAGC AGCAGCTGCA GCAACAACAA CAACTGCAGC AGCAGCACCG GCAGTACCAA 
ATGGACCAAT TGCCAGACTA CTATGATACC CCACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phlpp1 ( XM_004915308.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915365.1 Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X1, mRNA.

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CloneID	OXa11757
Clone ID Related Accession (Same CDS sequence)	XM_004915308.3
Accession Version	XM_004915308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915308.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCTGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAATGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAG CAACATTCGG 
TTCTTTAAGG TGGACCAGCC ATCCTGTGGG TTCTCTGGAA GTGAAGCATC AGGAGTGCCA 
GCTGTGTGGA GCCATGGATA TGCAGAGGCA TCAGGTGTTA AGAGCAAGCT TTGTGTAGCA 
TCCCTTTTGG TCAACAATTT CTGTGATAAC CGTGAAGCTG TGTATGGTGT GTTTGATGGT 
GAGCGGAATG TCGAGGTACC CAGCCTGCTG CAGTGCACAA TGAGTGACAT ACTAGCTGAT 
GAACTGCAAA AAACAAAGAG CGAGGAGGAG TACATGGGAA ATACCTTCCT GGTTATGCAG 
AGGAAACTGG GTACAGCTGG ACAAAAACTA GGGGGCTCTG CTGTCCTCTG CCACATAAAG 
CATGATCCCA TGGACCCTGC TGGATGTTTC ACGCTCACCA CAGCCAATGT AGGCAAGTGC 
CAAACTGTTA TCTGCAGAGA TGGGAAGCCG CTTAACCTGT CCAAACACCT TACAGTCAGC 
TGTGAAGAAG AATTAAAGAG AATAAAACAA CACAAGGCCA TCATCACAGA GGATGGAAAA 
GTTAATGGAG TGACGGATTC AACACGGATA CTGGGTTATA CATTTCTTCA CCCAAGCGTC 
ACCCCCCGGC CGCATGTACA GTCTGTTACT CTAACACCAC AGGACGAATT CTTCATCCTA 
GGAAGCAAGG GTTTGTGGGA TAGCCTTTCA ATGGAGGAAG CTGTTGAAAC AGTGAGGAAT 
GTACCAGATG CCCTGGCAGC TTCTAAGAAG TTGTGCAGCT TAGCCCAAAG CTATGGGTGC 
AGCGGTAGTT TGTGTGCAGT GGTGGTGCAG CTAAATGTCA CTGAAGACTG TATTTGCTGC 
TGTGAGCTAA ATGGCGGCAT GCCACCTCCC AGCCCTGGAA TTTTTACCCC ATCTGTCAAC 
GTGGTTATCA AGGACCGGGC CCCTGACAGC CTTGGCATGC CATCTTCCAG TAGTGGCTTA 
GCCTCAGAAA TAAGCAGTGA GATATCCACT TCAGAGATGA GTAGTGAAGT TGGGTCGACT 
GCTTCTGATG AACCCCCTGC AGTTTCTCTT CATGAAAATG GAGTGGGTCC TGTTGGTTGT 
CCAACTGAGC AGCGTTGTAC TCTACATCCA GTCTGTCTTT CAAGTTCCTT CCAGAGACAG 
CTCTCTAGTG CAACGTTCTC TAGTGCCTTC TCTGACAATG GACTGGACAG TGAAGATGAG 
GAGCCAATAG AAGGTGTCTT CTCTAATGGG AGCCGAGTGG AGGTAGAAGT GGATATCCAT 
TGTTCTCGAA CAAAGTCTGG ATGCCAGACT GTACCTGCAG TACTTACAGA GTCTACCGAC 
GAGGGTATTG TCATCAATGG CAATGAGGAT GACATGGGAG GTCCTGGTAA GAGACAAGAC 
TTTTCCTCAA ATACTCTCGG TCGACAGAGA AGAGGCAATG GTTCAGTTGC ACCCTTAGAA 
AAGAGCCACA ACCTTATTGA AATTGCAGCA GATGCTCCTC TTAGAAAGCA AGGGGGCTAT 
TTTGCAGCCC CTGCCCAGCC TGATCCTGAT GACCAATTCA TTATACCTCC TGAACTGGAG 
GAAGAAGTTA AGGAGATTAT GAAACAACAT CAGGAGCAGC AACAACAACA ACAACAACAA 
CAGCAACAAC AGCAGCAGCT GCAGCAGCAG CACCGGCAGT ACCAAATGGA CCAATTGCCA 
GACTACTATG ATACCCCACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915365.1
CDS	713..5296
Translation	MLAHSPVRAAKVRVVETGAPRSMEQGAAEEPAAGPGTEEKKRPPVSAGNSTTGTLLLPPATNGSTSANSVLLLRRRRRRLKRNVSAIHSPSPLSSCPPGPASRSLERRAPLASRSRQWVPLQAQDRQCVRSELRRGCIHVYERHASPCLRPLICTLDTQSNQVATRLLRTGGSSGVLRILGPETSEQGSYFVAGVKGLGHCWEPGDEAPHQPASDSDSLSPTVCQGLELFHCGAASSSSSSSSSSVEELGMPTIPRAVTVPARPSREAAQCPASLYVQLHGESCRRLEAQEKPLQIQNDYLFQLGFRDLCRVQEEGMEPEIGCLIRFYAGKSNCGGFSERTQLSGTYNVRKGKMQLPVNRWARRQVILCGTCLIVSSVKESQTGKMHILPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTDYLRWLRQASKIASQRTSSVDLSCCSLEYLPENLFYSQDITHLNLKQNILRLSPGPAAEGTLDDLQRFTKLRSLNLSNNNLGSFPLAVCSIPTLTELNLSCNALRAIPSQVGDMNNLQTFLLDGNYLQSLPAEMGNMHQLNYLGLSFNEFTDIPEVVEKMASMDRLCMPGNALDILELQTLRKMSQIKHVDLRLNEIRGLVIDEPDFLPHITQLDLRDNKLTDLDVTALSNLEVLHCERNRLRCLKISGFFLKALYASSNELLNLDVYPNPCYLSYMDISRNCLHSLPEWFSECRNLEVLDVSHNHLNELPDRLFCTSALRKLLLGYNQLQRLPELIENNQLEVLDVQHNQLLELPANLLLKLNSLRCLNASANHLETLPATCLSEESHSMLQELYLTNNNLTDKCVPLLMGHPHLKILHMAFNHLQTFPASKMAKLEELEEIDISGNKLKAMPTTIMNCRRMHTVIAHSNCLEVFPEVMQLSEIKCVDLSCNELTEITLPENLPPRLQELDLTGNARLLLDHKTLELLNSNIRFFKVDQPSCGFSGSEASGVPAVWSHGYAEASGVKSKLCVASLLVNNFCDNREAVYGVFDGERNVEVPSLLQCTMSDILADELQKTKSEEEYMGNTFLVMQRKLGTAGQKLGGSAVLCHIKHDPMDPAGCFTLTTANVGKCQTVICRDGKPLNLSKHLTVSCEEELKRIKQHKAIITEDGKVNGVTDSTRILGYTFLHPSVTPRPHVQSVTLTPQDEFFILGSKGLWDSLSMEEAVETVRNVPDALAASKKLCSLAQSYGCSGSLCAVVVQLNVTEDCICCCELNGGMPPPSPGIFTPSVNVVIKDRAPDSLGMPSSSSGLASEISSEISTSEMSSEVGSTASDEPPAVSLHENGVGPVGCPTEQRCTLHPVCLSSSFQRQLSSATFSSAFSDNGLDSEDEEPIEGVFSNGSRVEVEVDIHCSRTKSGCQTVPAVLTESTDEGIVINGNEDDMGGPGKRQDFSSNTLGRQRRGNGSVAPLEKSHNLIEIAADAPLRKQGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKQHQEQQQQQQQQQQQQQQLQQQHRQYQMDQLPDYYDTPL

Target ORF information:

RefSeq Version	XM_004915308.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915308.3

ORF Insert Sequence:

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCTGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAATGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAG CAACATTCGG 
TTCTTTAAGG TGGACCAGCC ATCCTGTGGG TTCTCTGGAA GTGAAGCATC AGGAGTGCCA 
GCTGTGTGGA GCCATGGATA TGCAGAGGCA TCAGGTGTTA AGAGCAAGCT TTGTGTAGCA 
TCCCTTTTGG TCAACAATTT CTGTGATAAC CGTGAAGCTG TGTATGGTGT GTTTGATGGT 
GAGCGGAATG TCGAGGTACC CAGCCTGCTG CAGTGCACAA TGAGTGACAT ACTAGCTGAT 
GAACTGCAAA AAACAAAGAG CGAGGAGGAG TACATGGGAA ATACCTTCCT GGTTATGCAG 
AGGAAACTGG GTACAGCTGG ACAAAAACTA GGGGGCTCTG CTGTCCTCTG CCACATAAAG 
CATGATCCCA TGGACCCTGC TGGATGTTTC ACGCTCACCA CAGCCAATGT AGGCAAGTGC 
CAAACTGTTA TCTGCAGAGA TGGGAAGCCG CTTAACCTGT CCAAACACCT TACAGTCAGC 
TGTGAAGAAG AATTAAAGAG AATAAAACAA CACAAGGCCA TCATCACAGA GGATGGAAAA 
GTTAATGGAG TGACGGATTC AACACGGATA CTGGGTTATA CATTTCTTCA CCCAAGCGTC 
ACCCCCCGGC CGCATGTACA GTCTGTTACT CTAACACCAC AGGACGAATT CTTCATCCTA 
GGAAGCAAGG GTTTGTGGGA TAGCCTTTCA ATGGAGGAAG CTGTTGAAAC AGTGAGGAAT 
GTACCAGATG CCCTGGCAGC TTCTAAGAAG TTGTGCAGCT TAGCCCAAAG CTATGGGTGC 
AGCGGTAGTT TGTGTGCAGT GGTGGTGCAG CTAAATGTCA CTGAAGACTG TATTTGCTGC 
TGTGAGCTAA ATGGCGGCAT GCCACCTCCC AGCCCTGGAA TTTTTACCCC ATCTGTCAAC 
GTGGTTATCA AGGACCGGGC CCCTGACAGC CTTGGCATGC CATCTTCCAG TAGTGGCTTA 
GCCTCAGAAA TAAGCAGTGA GATATCCACT TCAGAGATGA GTAGTGAAGT TGGGTCGACT 
GCTTCTGATG AACCCCCTGC AGTTTCTCTT CATGAAAATG GAGTGGGTCC TGTTGGTTGT 
CCAACTGAGC AGCGTTGTAC TCTACATCCA GTCTGTCTTT CAAGTTCCTT CCAGAGACAG 
CTCTCTAGTG CAACGTTCTC TAGTGCCTTC TCTGACAATG GACTGGACAG TGAAGATGAG 
GAGCCAATAG AAGGTGTCTT CTCTAATGGG AGCCGAGTGG AGGTAGAAGT GGATATCCAT 
TGTTCTCGAA CAAAGTCTGG ATGCCAGACT GTACCTGCAG TACTTACAGA GTCTACCGAC 
GAGGGTATTG TCATCAATGG CAATGAGGAT GACATGGGAG GTCCTGGTAA GAGACAAGAC 
TTTTCCTCAA ATACTCTCGG TCGACAGAGA AGAGGCAATG GTTCAGTTGC ACCCTTAGAA 
AAGAGCCACA ACCTTATTGA AATTGCAGCA GATGCTCCTC TTAGAAAGCA AGGGGGCTAT 
TTTGCAGCCC CTGCCCAGCC TGATCCTGAT GACCAATTCA TTATACCTCC TGAACTGGAG 
GAAGAAGTTA AGGAGATTAT GAAACAACAT CAGGAGCAGC AACAACAACA ACAACAACAA 
CAGCAACAAC AGCAGCAGCT GCAGCAGCAG CACCGGCAGT ACCAAATGGA CCAATTGCCA 
GACTACTATG ATACCCCACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phlpp1 ( XM_002934390.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934436.2 Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X2, mRNA.

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CloneID	OXa11758
Clone ID Related Accession (Same CDS sequence)	XM_002934390.4
Accession Version	XM_002934390.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934390.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCTGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAATGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAA CATTCGGTTC 
TTTAAGGTGG ACCAGCCATC CTGTGGGTTC TCTGGAAGTG AAGCATCAGG AGTGCCAGCT 
GTGTGGAGCC ATGGATATGC AGAGGCATCA GGTGTTAAGA GCAAGCTTTG TGTAGCATCC 
CTTTTGGTCA ACAATTTCTG TGATAACCGT GAAGCTGTGT ATGGTGTGTT TGATGGTGAG 
CGGAATGTCG AGGTACCCAG CCTGCTGCAG TGCACAATGA GTGACATACT AGCTGATGAA 
CTGCAAAAAA CAAAGAGCGA GGAGGAGTAC ATGGGAAATA CCTTCCTGGT TATGCAGAGG 
AAACTGGGTA CAGCTGGACA AAAACTAGGG GGCTCTGCTG TCCTCTGCCA CATAAAGCAT 
GATCCCATGG ACCCTGCTGG ATGTTTCACG CTCACCACAG CCAATGTAGG CAAGTGCCAA 
ACTGTTATCT GCAGAGATGG GAAGCCGCTT AACCTGTCCA AACACCTTAC AGTCAGCTGT 
GAAGAAGAAT TAAAGAGAAT AAAACAACAC AAGGCCATCA TCACAGAGGA TGGAAAAGTT 
AATGGAGTGA CGGATTCAAC ACGGATACTG GGTTATACAT TTCTTCACCC AAGCGTCACC 
CCCCGGCCGC ATGTACAGTC TGTTACTCTA ACACCACAGG ACGAATTCTT CATCCTAGGA 
AGCAAGGGTT TGTGGGATAG CCTTTCAATG GAGGAAGCTG TTGAAACAGT GAGGAATGTA 
CCAGATGCCC TGGCAGCTTC TAAGAAGTTG TGCAGCTTAG CCCAAAGCTA TGGGTGCAGC 
GGTAGTTTGT GTGCAGTGGT GGTGCAGCTA AATGTCACTG AAGACTGTAT TTGCTGCTGT 
GAGCTAAATG GCGGCATGCC ACCTCCCAGC CCTGGAATTT TTACCCCATC TGTCAACGTG 
GTTATCAAGG ACCGGGCCCC TGACAGCCTT GGCATGCCAT CTTCCAGTAG TGGCTTAGCC 
TCAGAAATAA GCAGTGAGAT ATCCACTTCA GAGATGAGTA GTGAAGTTGG GTCGACTGCT 
TCTGATGAAC CCCCTGCAGT TTCTCTTCAT GAAAATGGAG TGGGTCCTGT TGGTTGTCCA 
ACTGAGCAGC GTTGTACTCT ACATCCAGTC TGTCTTTCAA GTTCCTTCCA GAGACAGCTC 
TCTAGTGCAA CGTTCTCTAG TGCCTTCTCT GACAATGGAC TGGACAGTGA AGATGAGGAG 
CCAATAGAAG GTGTCTTCTC TAATGGGAGC CGAGTGGAGG TAGAAGTGGA TATCCATTGT 
TCTCGAACAA AGTCTGGATG CCAGACTGTA CCTGCAGTAC TTACAGAGTC TACCGACGAG 
GGTATTGTCA TCAATGGCAA TGAGGATGAC ATGGGAGGTC CTGGTAAGAG ACAAGACTTT 
TCCTCAAATA CTCTCGGTCG ACAGAGAAGA GGCAATGGTT CAGTTGCACC CTTAGAAAAG 
AGCCACAACC TTATTGAAAT TGCAGCAGAT GCTCCTCTTA GAAAGCAAGG GGGCTATTTT 
GCAGCCCCTG CCCAGCCTGA TCCTGATGAC CAATTCATTA TACCTCCTGA ACTGGAGGAA 
GAAGTTAAGG AGATTATGAA ACAACATCAG GAGCAGCAAC AACAACAACA ACAACAACAG 
CAACAACAGC AGCAGCTGCA GCAGCAGCAC CGGCAGTACC AAATGGACCA ATTGCCAGAC 
TACTATGATA CCCCACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934436.2
CDS	719..5299
Translation	MLAHSPVRAAKVRVVETGAPRSMEQGAAEEPAAGPGTEEKKRPPVSAGNSTTGTLLLPPATNGSTSANSVLLLRRRRRRLKRNVSAIHSPSPLSSCPPGPASRSLERRAPLASRSRQWVPLQAQDRQCVRSELRRGCIHVYERHASPCLRPLICTLDTQSNQVATRLLRTGGSSGVLRILGPETSEQGSYFVAGVKGLGHCWEPGDEAPHQPASDSDSLSPTVCQGLELFHCGAASSSSSSSSSSVEELGMPTIPRAVTVPARPSREAAQCPASLYVQLHGESCRRLEAQEKPLQIQNDYLFQLGFRDLCRVQEEGMEPEIGCLIRFYAGKSNCGGFSERTQLSGTYNVRKGKMQLPVNRWARRQVILCGTCLIVSSVKESQTGKMHILPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTDYLRWLRQASKIASQRTSSVDLSCCSLEYLPENLFYSQDITHLNLKQNILRLSPGPAAEGTLDDLQRFTKLRSLNLSNNNLGSFPLAVCSIPTLTELNLSCNALRAIPSQVGDMNNLQTFLLDGNYLQSLPAEMGNMHQLNYLGLSFNEFTDIPEVVEKMASMDRLCMPGNALDILELQTLRKMSQIKHVDLRLNEIRGLVIDEPDFLPHITQLDLRDNKLTDLDVTALSNLEVLHCERNRLRCLKISGFFLKALYASSNELLNLDVYPNPCYLSYMDISRNCLHSLPEWFSECRNLEVLDVSHNHLNELPDRLFCTSALRKLLLGYNQLQRLPELIENNQLEVLDVQHNQLLELPANLLLKLNSLRCLNASANHLETLPATCLSEESHSMLQELYLTNNNLTDKCVPLLMGHPHLKILHMAFNHLQTFPASKMAKLEELEEIDISGNKLKAMPTTIMNCRRMHTVIAHSNCLEVFPEVMQLSEIKCVDLSCNELTEITLPENLPPRLQELDLTGNARLLLDHKTLELLNNIRFFKVDQPSCGFSGSEASGVPAVWSHGYAEASGVKSKLCVASLLVNNFCDNREAVYGVFDGERNVEVPSLLQCTMSDILADELQKTKSEEEYMGNTFLVMQRKLGTAGQKLGGSAVLCHIKHDPMDPAGCFTLTTANVGKCQTVICRDGKPLNLSKHLTVSCEEELKRIKQHKAIITEDGKVNGVTDSTRILGYTFLHPSVTPRPHVQSVTLTPQDEFFILGSKGLWDSLSMEEAVETVRNVPDALAASKKLCSLAQSYGCSGSLCAVVVQLNVTEDCICCCELNGGMPPPSPGIFTPSVNVVIKDRAPDSLGMPSSSSGLASEISSEISTSEMSSEVGSTASDEPPAVSLHENGVGPVGCPTEQRCTLHPVCLSSSFQRQLSSATFSSAFSDNGLDSEDEEPIEGVFSNGSRVEVEVDIHCSRTKSGCQTVPAVLTESTDEGIVINGNEDDMGGPGKRQDFSSNTLGRQRRGNGSVAPLEKSHNLIEIAADAPLRKQGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKQHQEQQQQQQQQQQQQQQLQQQHRQYQMDQLPDYYDTPL

Target ORF information:

RefSeq Version	XM_002934390.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis PH domain and leucine rich repeat protein phosphatase 1 (phlpp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934390.4

ORF Insert Sequence:

ATGCTTGCGC ATTCCCCGGT GAGGGCCGCC AAGGTTCGCG TCGTAGAAAC TGGGGCACCG 
CGATCCATGG AACAGGGAGC GGCCGAAGAA CCTGCGGCGG GGCCGGGGAC GGAGGAGAAA 
AAGCGACCGC CGGTCAGTGC AGGGAACTCA ACTACGGGCA CTTTGCTGCT GCCCCCGGCG 
ACTAATGGCA GTACGAGCGC CAACTCCGTA CTCCTTCTGC GGAGGAGAAG GCGAAGGCTT 
AAGAGGAACG TGTCGGCCAT CCATTCCCCG TCTCCCCTGT CTTCCTGCCC GCCGGGGCCC 
GCCTCCCGCA GCCTAGAACG GAGGGCACCC CTGGCATCGC GGAGCAGACA GTGGGTGCCT 
CTGCAGGCAC AGGACAGGCA GTGTGTGCGC TCAGAGCTGC GGAGAGGCTG CATACATGTG 
TACGAGCGGC ACGCCTCCCC CTGCCTGCGG CCGCTTATCT GCACTTTGGA CACCCAGTCT 
AACCAAGTGG CGACCCGCCT GCTTCGGACG GGAGGGAGTT CGGGGGTACT GAGAATCCTG 
GGCCCCGAGA CGAGCGAGCA GGGCTCGTAC TTTGTGGCTG GAGTTAAGGG CCTGGGACAT 
TGTTGGGAAC CGGGGGATGA AGCGCCGCAC CAGCCGGCCT CGGACTCCGA CAGTCTGAGT 
CCTACAGTGT GCCAGGGACT GGAGCTCTTC CACTGCGGGG CAGCCTCATC CTCCTCCTCC 
TCCTCTTCCT CCTCAGTGGA AGAACTGGGG ATGCCCACAA TTCCCCGGGC AGTGACAGTG 
CCTGCCCGAC CCAGCCGGGA GGCAGCCCAG TGTCCGGCAT CCCTGTATGT TCAGCTACAC 
GGGGAGAGCT GCCGCAGGTT GGAAGCACAG GAGAAGCCTC TGCAAATCCA GAACGACTAC 
CTCTTTCAGC TGGGCTTTAG GGATCTGTGC AGGGTGCAAG AGGAGGGCAT GGAGCCAGAG 
ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAGTCAAACT GTGGTGGTTT CTCTGAACGG 
ACTCAACTTT CGGGAACGTA TAATGTTCGT AAAGGGAAGA TGCAGCTGCC TGTAAACAGA 
TGGGCACGAC GGCAAGTGAT TCTTTGTGGA ACTTGTCTAA TTGTATCATC GGTGAAGGAA 
AGCCAGACTG GAAAGATGCA TATTCTACCT TTAATAGGTG GAAAAGTGGA AGAAGTAAAG 
AAGCACCAGC ACTGCCTGGC ATTCAGCTCA TCTGGTCCAC AGAGTCAAAC ATACTATATT 
TGCTTTGACA CATTCACAGA TTACTTGAGA TGGCTCCGAC AGGCTTCTAA GATTGCATCT 
CAGCGCACCA GTTCTGTGGA TCTGTCCTGC TGCAGCTTGG AATATCTCCC AGAGAACCTG 
TTCTACAGCC AAGATATCAC ACACCTCAAC CTGAAACAAA ATATTCTCAG ACTCTCTCCA 
GGTCCAGCAG CAGAAGGCAC TCTTGATGAT CTTCAGAGGT TTACAAAGTT GAGGAGTCTC 
AATCTTTCCA ATAATAATTT GGGTAGTTTC CCACTGGCTG TTTGCAGTAT CCCTACTCTG 
ACAGAACTCA ACCTTTCCTG TAATGCTCTT CGGGCCATTC CTTCACAGGT TGGAGACATG 
AACAATTTGC AGACGTTCCT TTTGGATGGC AACTACCTTC AGTCTCTCCC TGCTGAAATG 
GGAAACATGC ACCAGCTAAA CTATTTGGGC CTCTCATTTA ATGAATTTAC AGACATTCCT 
GAGGTGGTGG AAAAGATGGC TTCCATGGAT AGATTGTGTA TGCCAGGGAA CGCTTTGGAT 
ATCCTAGAGT TGCAGACTCT GAGGAAGATG TCACAGATTA AGCATGTAGA TTTAAGGCTA 
AATGAAATCA GAGGATTGGT AATAGATGAG CCAGATTTTT TGCCTCACAT CACCCAGTTG 
GACCTCCGAG ATAACAAGTT GACTGATCTT GATGTGACTG CCCTCAGCAA CTTAGAAGTC 
CTGCACTGTG AGAGGAACAG ACTAAGGTGT CTTAAAATCA GTGGCTTCTT CTTAAAAGCA 
CTTTATGCAT CTTCTAATGA ACTCCTAAAT CTTGATGTGT ATCCAAACCC TTGTTATTTA 
TCATACATGG ACATTTCTAG AAACTGTCTG CATTCCCTTC CTGAGTGGTT CAGTGAATGC 
AGAAATTTAG AGGTACTGGA TGTGAGCCAC AATCACCTAA ATGAGCTTCC TGACCGGTTA 
TTCTGTACCA GTGCTTTGAG AAAGCTACTT TTAGGTTACA ACCAGCTGCA AAGGCTTCCA 
GAATTAATTG AGAACAACCA ACTTGAGGTA CTTGATGTGC AGCACAACCA GCTATTGGAG 
CTCCCTGCCA ACTTGCTGCT TAAATTAAAC AGCCTAAGGT GCCTCAATGC CTCTGCCAAC 
CATTTAGAAA CCCTTCCTGC CACATGTCTG TCCGAAGAGT CGCACAGTAT GTTGCAGGAG 
CTGTATCTGA CTAATAATAA TCTCACTGAC AAATGTGTGC CCTTATTAAT GGGACATCCC 
CACCTGAAGA TCCTGCACAT GGCCTTCAAT CATCTACAGA CTTTCCCAGC AAGTAAAATG 
GCCAAACTGG AGGAACTAGA AGAAATAGAT ATTAGCGGGA ACAAGCTGAA AGCCATGCCA 
ACTACCATAA TGAACTGCAG GCGCATGCAC ACAGTCATTG CTCACTCCAA CTGCCTGGAA 
GTCTTCCCTG AAGTTATGCA GCTATCCGAG ATTAAGTGTG TGGACTTAAG CTGCAATGAA 
CTTACTGAAA TCACGTTGCC TGAAAACCTA CCTCCCAGAC TGCAGGAACT GGACCTGACT 
GGTAATGCCC GCTTGCTCCT TGACCACAAA ACCCTGGAGC TTCTGAACAA CATTCGGTTC 
TTTAAGGTGG ACCAGCCATC CTGTGGGTTC TCTGGAAGTG AAGCATCAGG AGTGCCAGCT 
GTGTGGAGCC ATGGATATGC AGAGGCATCA GGTGTTAAGA GCAAGCTTTG TGTAGCATCC 
CTTTTGGTCA ACAATTTCTG TGATAACCGT GAAGCTGTGT ATGGTGTGTT TGATGGTGAG 
CGGAATGTCG AGGTACCCAG CCTGCTGCAG TGCACAATGA GTGACATACT AGCTGATGAA 
CTGCAAAAAA CAAAGAGCGA GGAGGAGTAC ATGGGAAATA CCTTCCTGGT TATGCAGAGG 
AAACTGGGTA CAGCTGGACA AAAACTAGGG GGCTCTGCTG TCCTCTGCCA CATAAAGCAT 
GATCCCATGG ACCCTGCTGG ATGTTTCACG CTCACCACAG CCAATGTAGG CAAGTGCCAA 
ACTGTTATCT GCAGAGATGG GAAGCCGCTT AACCTGTCCA AACACCTTAC AGTCAGCTGT 
GAAGAAGAAT TAAAGAGAAT AAAACAACAC AAGGCCATCA TCACAGAGGA TGGAAAAGTT 
AATGGAGTGA CGGATTCAAC ACGGATACTG GGTTATACAT TTCTTCACCC AAGCGTCACC 
CCCCGGCCGC ATGTACAGTC TGTTACTCTA ACACCACAGG ACGAATTCTT CATCCTAGGA 
AGCAAGGGTT TGTGGGATAG CCTTTCAATG GAGGAAGCTG TTGAAACAGT GAGGAATGTA 
CCAGATGCCC TGGCAGCTTC TAAGAAGTTG TGCAGCTTAG CCCAAAGCTA TGGGTGCAGC 
GGTAGTTTGT GTGCAGTGGT GGTGCAGCTA AATGTCACTG AAGACTGTAT TTGCTGCTGT 
GAGCTAAATG GCGGCATGCC ACCTCCCAGC CCTGGAATTT TTACCCCATC TGTCAACGTG 
GTTATCAAGG ACCGGGCCCC TGACAGCCTT GGCATGCCAT CTTCCAGTAG TGGCTTAGCC 
TCAGAAATAA GCAGTGAGAT ATCCACTTCA GAGATGAGTA GTGAAGTTGG GTCGACTGCT 
TCTGATGAAC CCCCTGCAGT TTCTCTTCAT GAAAATGGAG TGGGTCCTGT TGGTTGTCCA 
ACTGAGCAGC GTTGTACTCT ACATCCAGTC TGTCTTTCAA GTTCCTTCCA GAGACAGCTC 
TCTAGTGCAA CGTTCTCTAG TGCCTTCTCT GACAATGGAC TGGACAGTGA AGATGAGGAG 
CCAATAGAAG GTGTCTTCTC TAATGGGAGC CGAGTGGAGG TAGAAGTGGA TATCCATTGT 
TCTCGAACAA AGTCTGGATG CCAGACTGTA CCTGCAGTAC TTACAGAGTC TACCGACGAG 
GGTATTGTCA TCAATGGCAA TGAGGATGAC ATGGGAGGTC CTGGTAAGAG ACAAGACTTT 
TCCTCAAATA CTCTCGGTCG ACAGAGAAGA GGCAATGGTT CAGTTGCACC CTTAGAAAAG 
AGCCACAACC TTATTGAAAT TGCAGCAGAT GCTCCTCTTA GAAAGCAAGG GGGCTATTTT 
GCAGCCCCTG CCCAGCCTGA TCCTGATGAC CAATTCATTA TACCTCCTGA ACTGGAGGAA 
GAAGTTAAGG AGATTATGAA ACAACATCAG GAGCAGCAAC AACAACAACA ACAACAACAG 
CAACAACAGC AGCAGCTGCA GCAGCAGCAC CGGCAGTACC AAATGGACCA ATTGCCAGAC 
TACTATGATA CCCCACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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phlpp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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