PHLPP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHLPP1
Entrez Gene ID	23239
Full Name	PH domain and leucine rich repeat protein phosphatase 1
Synonyms	PHLPP,PLEKHE1,PPM3A,SCOP
General protein information	Preferred Names PH domain and leucine rich repeat protein phosphatase 1 Names PH domain leucine-rich repeat-containing protein phosphatase 1 PH domain-containing family E member 1 SCN circadian oscillatory protein pleckstrin homology domain containing, family E (with leucine rich repeats) member 1 protein phosphatase, Mg2+/Mn2+ dependent 3A suprachiasmatic nucleus circadian oscillatory protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q21.33
Summary	This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011].
Disorder MIM:	609396

mRNA and Protein(s)

mRNA	Protein	Name
XM_024451105.1	XP_024306873.1	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X1
NM_194449.3	NP_919431.2	PH domain leucine-rich repeat-containing protein phosphatase 1
XM_011525886.1	XP_011524188.1	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X2
NM_194449.4	NP_919431.2	PH domain leucine-rich repeat-containing protein phosphatase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHLPP1	XP_523952.2
Rattus norvegicus (Norway rat)	Phlpp1	NP_067689.1
Mus musculus (house mouse)	Phlpp1	NP_598582.3
Canis lupus familiaris (dog)	PHLPP1	XP_005615724.1
Bos taurus (cattle)	PHLPP1	XP_002707832.2
Xenopus tropicalis (tropical clawed frog)	LOC100497530	XP_004915365.1
Homo sapiens (human)	PHLPP1	NP_919431.2
	PHLPP1	XP_001094216.1
Gallus gallus (chicken)	PHLPP1	XP_418990.4

Gene Ontology
Bibliography

Related articles in PubMed

Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1.
Gangula NR, Maddika S
The Journal of biological chemistry292(34)13947-13958(2017 08)

LPS depletes PHLPP levels in macrophages through the inhibition of SP1 dependent transcriptional regulation.
Alamuru-Yellapragada NP, Vundyala S, Behera S, Parsa KV
Biochemical and biophysical research communications486(2)533-538(2017 04)

Increased levels of the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP)-1 in obese participants are associated with insulin resistance.
Andreozzi F, Procopio C, Greco A, Mannino GC, Miele C, Raciti GA, Iadicicco C, Beguinot F, Pontiroli AE, Hribal ML, Folli F, Sesti G
Diabetologia54(7)1879-87(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that PHLPP1 plays an important role in assembly of kinetochores by counteracting RNF41-mediated SGT1 degradation. (PHLPP1 = PH domain and leucine rich repeat protein phosphatase 1; RNF41 = ring finger protein 41; SGT1 = suppressor of G2 allele of SKP1)
Title: Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1.

nickel exposure results in DNMT3b induction and MEG3 promoter hypermethylation and expression inhibition, further reduces its binding to c-Jun and in turn increasing c-Jun inhibition of PHLPP1 transcription, leading to the Akt/p70S6K/S6 axis activation, and HIF-1alpha protein translation, as well as malignant transformation of human bronchial epithelial cells.
Title: LncRNA MEG3 downregulation mediated by DNMT3b contributes to nickel malignant transformation of human bronchial epithelial cells via modulating PHLPP1 transcription and HIF-1? translation.

During LPS induced macrophage inflammatory response, PHLPP levels in macrophages are depleted through the inhibition of SP1 dependent transcriptional regulation.
Title: LPS depletes PHLPP levels in macrophages through the inhibition of SP1 dependent transcriptional regulation.

Low PHLPP expression is associated with gallbladder cancer.
Title: SNORA74B gene silencing inhibits gallbladder cancer cells by inducing PHLPP and suppressing Akt/mTOR signaling.

Abnormal PHLPP, FoxO3a, and RAD51 protein expressions may be involved in the development of high- and low-grade ovarian serous adenocarcinomas, suggesting common molecular pathways.
Title: Expression of PH Domain Leucine-rich Repeat Protein Phosphatase, Forkhead Homeobox Type O 3a and RAD51, and their Relationships with Clinicopathologic Features and Prognosis in Ovarian Serous Adenocarcinoma.

The following PHLPP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLPP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104512	XM_024451105.1 Latest version!	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu21282	NM_194449.4 Latest version!	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu65770	XM_011525886.1 Latest version!	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OHu21282	NM_194449.3	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHLPP1 ( XM_024451105.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306873.1 Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104512
Clone ID Related Accession (Same CDS sequence)	XM_024451105.1
Accession Version	XM_024451105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATAATG TCCGTAAAGG CAAGATGCAG TTGCCAGTGA ACCGATGGAC AAGACGCCAA 
GTCATCCTAT GTGGGACCTG CCTGATAGTA TCATCTGTGA AAGACAGCTT GACCGGAAAG 
ATGCATGTTC TGCCACTAAT TGGTGGAAAA GTAGAAGAAG TGAAAAAGCA CCAACACTGT 
TTAGCATTTA GCTCCTCTGG ACCCCAAAGC CAGACTTACT ACATTTGCTT TGATACTTTC 
ACAGAATACT TAAGGTGGCT GCGACAAGTC TCCAAGGTTG CATCCCAGCG CATTAGCTCA 
GTGGACCTCT CGTGTTGTAG CCTGGAACAT CTGCCTGCCA ACCTCTTCTA CAGCCAAGAC 
CTCACTCATC TCAATTTAAA ACAAAACTTC CTAAGGCAGA ACCCTAGCCT TCCAGCTGCC 
AGGGGGCTTA ATGAACTGCA AAGGTTCACC AAGTTGAAGA GTCTTAACCT TTCCAATAAT 
CATTTAGGGG ACTTCCCACT GGCAGTCTGC AGTATTCCAA CCCTGGCAGA GCTGAACGTG 
TCCTGCAATG CCCTGCGATC AGTCCCGGCA GCCGTTGGAG TGATGCACAA CTTACAGACA 
TTTTTGTTGG ATGGAAACTT TCTCCAATCC CTTCCTGCTG AGTTGGAGAA CATGAAGCAG 
CTTAGTTATC TGGGTCTTTC TTTCAATGAA TTTACTGACA TTCCCGAAGT ATTGGAGAAA 
TTGACTGCTG TGGATAAACT TTGTATGTCT GGAAACTGTG TGGAGACCCT TAGGCTACAG 
GCTTTAAGAA AAATGCCTCA CATTAAACAT GTGGATCTAA GGTTGAACGT AATTAGGAAG 
CTGATAGCAG ATGAAGTGGA CTTTCTACAG CATGTTACTC AGCTTGACCT ACGAGACAAT 
AAGCTTGGTG ATCTAGATGC TATGATTTTC AACAACATTG AAGTTTTACA CTGTGAAAGG 
AATCAACTGG TCACATTAGA CATCTGTGGC TATTTCCTAA AAGCGCTCTA TGCCTCTTCT 
AATGAACTTG TTCAACTTGA TGTTTACCCA GTTCCAAATT ATCTGTCCTA CATGGATGTT 
TCAAGGAACC GCTTAGAAAA TGTGCCTGAG TGGGTATGTG AAAGCCGAAA GCTAGAAGTT 
TTGGATATTG GCCATAATCA AATATGTGAA CTTCCTGCCC GCTTATTTTG TAATAGCAGT 
CTCCGGAAAC TACTGGCAGG ACACAACCAG TTGGCAAGGC TGCCTGAAAG GCTAGAAAGA 
ACCTCGGTGG AGGTCTTGGA TGTGCAACAC AACCAGCTCC TTGAGCTCCC ACCTAACCTT 
CTGATGAAGG CTGACAGCCT GAGATTCCTG AACGCCTCTG CGAACAAACT GGAAAGCCTT 
CCTCCAGCCA CGCTTTCCGA AGAGACAAAC AGTATCTTAC AAGAGTTGTA TTTGACAAAT 
AACAGCCTCA CAGACAAATG TGTGCCCTTG TTAACGGGAC ACCCCCATTT GAAGATCCTT 
CACATGGCCT ATAACCGACT TCAGAGTTTT CCAGCAAGTA AAATGGCGAA ACTGGAGGAA 
CTTGAAGAAA TTGATCTCAG TGGGAATAAG CTGAAAGCCA TCCCAACAAC GATCATGAAT 
TGCAGGCGCA TGCACACCGT GATTGCTCAC TCCAACTGCA TCGAGGTCTT TCCCGAAGTT 
ATGCAGCTCC CAGAGATCAA GTGTGTGGAC CTGAGCTGTA ATGAGCTAAG TGAAGTCACA 
TTACCAGAAA ACCTGCCTCC CAAACTGCAG GAGCTAGACC TGACTGGAAA CCCGCGCCTT 
GTCCTTGATC ACAAAACCCT GGAACTACTG AATAATATCC GCTGTTTCAA GATTGATCAG 
CCTTCTACAG GAGACGCTTC CGGAGCCCCA GCTGTATGGA GTCATGGTTA CACTGAAGCT 
TCGGGGGTAA AAAACAAGTT GTGTGTCGCA GCCCTGTCGG TGAATAACTT CTGTGACAAC 
CGCGAAGCCC TGTATGGTGT GTTTGACGGA GACCGGAATG TGGAGGTGCC CTACCTTCTC 
CAGTGCACTA TGAGTGACAT TTTGGCTGAA GAGCTGCAAA AAACAAAAAA CGAAGAAGAA 
TACATGGTCA ATACATTCAT TGTCATGCAA AGGAAACTTG GAACTGCTGG GCAGAAGCTT 
GGTGGTGCCG CTGTCCTTTG TCATATCAAG CATGACCCTG TGGATCCAGG AGGATCCTTC 
ACCTTGACCT CTGCTAATGT GGGCAAGTGC CAAACAGTTC TCTGTCGAAA TGGAAAGCCG 
CTGCCTCTGT CCAGATCTTA CATCATGAGC TGTGAAGAAG AGCTGAAGAG GATTAAACAG 
CACAAGGCCA TTATCACTGA GGATGGCAAG GTGAACGGAG TGACTGAGTC CACGCGCATC 
CTGGGCTACA CCTTCCTCCA TCCCAGTGTG GTGCCTCGCC CCCACGTGCA GTCCGTGCTC 
CTGACTCCCC AGGATGAGTT CTTCATCCTA GGCAGTAAGG GGTTGTGGGA CAGCCTGTCC 
GTCGAGGAGG CCGTGGAAGC CGTGCGCAAC GTGCCCGATG CCCTGGCTGC TGCCAAGAAG 
CTGTGTACCC TGGCCCAGAG CTACGGCTGC CACGACAGCA TCAGCGCTGT GGTGGTGCAG 
CTCAGTGTCA CTGAGGACAG CTTCTGCTGC TGCGAGCTCA GCGCCGGTGG GGCTGTGCCA 
CCACCCAGTC CTGGCATCTT TCCTCCCTCA GTGAACATGG TGATCAAGGA TCGGCCCTCA 
GATGGGCTGG GCGTGCCGTC CTCCAGCAGC GGCATGGCTT CCGAGATTAG CAGTGAGCTC 
TCCACTTCTG AGATGAGCAG CGAGGTGGGG TCAACAGCCT CCGATGAGCC CCCGCCCGGA 
GCCCTAAGCG AGAACAGCCC TGCCTACCCC AGTGAGCAGC GCTGCATGCT CCACCCCATC 
TGTCTGTCCA ACTCCTTCCA GCGCCAGCTA TCCAGCGCCA CGTTCTCTAG CGCCTTCTCC 
GACAACGGCC TTGACAGTGA CGATGAGGAG CCCATCGAGG GCGTCTTCAC CAACGGCAGC 
CGGGTGGAGG TGGAGGTGGA CATCCACTGC AGCCGGGCCA AGGAGAAGGA GAAACAGCAG 
CACCTGCTTC AGGTGCCAGC AGAGGCCAGT GATGAGGGCA TTGTCATCAG CGCCAACGAG 
GATGAGCCAG GTCTGCCCAG GAAGGCAGAC TTCTCTGCCG TTGGGACCAT TGGGCGCCGG 
AGGGCCAATG GCTCTGTTGC GCCCCAGGAA AGGAGCCACA ATGTGATAGA GGTGGCTACA 
GACGCACCTC TTCGAAAGCC TGGAGGCTAT TTTGCTGCCC CGGCTCAGCC GGATCCTGAT 
GATCAGTTTA TCATACCCCC GGAGCTGGAA GAGGAGGTCA AAGAAATCAT GAAGCATCAC 
CAGGAGCAAC AGCAGCAGCA GCAGCCGCCA CCACCCCCTC AGCTCCAGCC GCAGCTGCCG 
CGGCACTACC AGCTGGACCA GCTGCCAGAT TATTACGACA CGCCACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306873.1
CDS	582..4112
Translation	MYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL

Target ORF information:

RefSeq Version	XM_024451105.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451105.1

ORF Insert Sequence:

ATGTATAATG TCCGTAAAGG CAAGATGCAG TTGCCAGTGA ACCGATGGAC AAGACGCCAA 
GTCATCCTAT GTGGGACCTG CCTGATAGTA TCATCTGTGA AAGACAGCTT GACCGGAAAG 
ATGCATGTTC TGCCACTAAT TGGTGGAAAA GTAGAAGAAG TGAAAAAGCA CCAACACTGT 
TTAGCATTTA GCTCCTCTGG ACCCCAAAGC CAGACTTACT ACATTTGCTT TGATACTTTC 
ACAGAATACT TAAGGTGGCT GCGACAAGTC TCCAAGGTTG CATCCCAGCG CATTAGCTCA 
GTGGACCTCT CGTGTTGTAG CCTGGAACAT CTGCCTGCCA ACCTCTTCTA CAGCCAAGAC 
CTCACTCATC TCAATTTAAA ACAAAACTTC CTAAGGCAGA ACCCTAGCCT TCCAGCTGCC 
AGGGGGCTTA ATGAACTGCA AAGGTTCACC AAGTTGAAGA GTCTTAACCT TTCCAATAAT 
CATTTAGGGG ACTTCCCACT GGCAGTCTGC AGTATTCCAA CCCTGGCAGA GCTGAACGTG 
TCCTGCAATG CCCTGCGATC AGTCCCGGCA GCCGTTGGAG TGATGCACAA CTTACAGACA 
TTTTTGTTGG ATGGAAACTT TCTCCAATCC CTTCCTGCTG AGTTGGAGAA CATGAAGCAG 
CTTAGTTATC TGGGTCTTTC TTTCAATGAA TTTACTGACA TTCCCGAAGT ATTGGAGAAA 
TTGACTGCTG TGGATAAACT TTGTATGTCT GGAAACTGTG TGGAGACCCT TAGGCTACAG 
GCTTTAAGAA AAATGCCTCA CATTAAACAT GTGGATCTAA GGTTGAACGT AATTAGGAAG 
CTGATAGCAG ATGAAGTGGA CTTTCTACAG CATGTTACTC AGCTTGACCT ACGAGACAAT 
AAGCTTGGTG ATCTAGATGC TATGATTTTC AACAACATTG AAGTTTTACA CTGTGAAAGG 
AATCAACTGG TCACATTAGA CATCTGTGGC TATTTCCTAA AAGCGCTCTA TGCCTCTTCT 
AATGAACTTG TTCAACTTGA TGTTTACCCA GTTCCAAATT ATCTGTCCTA CATGGATGTT 
TCAAGGAACC GCTTAGAAAA TGTGCCTGAG TGGGTATGTG AAAGCCGAAA GCTAGAAGTT 
TTGGATATTG GCCATAATCA AATATGTGAA CTTCCTGCCC GCTTATTTTG TAATAGCAGT 
CTCCGGAAAC TACTGGCAGG ACACAACCAG TTGGCAAGGC TGCCTGAAAG GCTAGAAAGA 
ACCTCGGTGG AGGTCTTGGA TGTGCAACAC AACCAGCTCC TTGAGCTCCC ACCTAACCTT 
CTGATGAAGG CTGACAGCCT GAGATTCCTG AACGCCTCTG CGAACAAACT GGAAAGCCTT 
CCTCCAGCCA CGCTTTCCGA AGAGACAAAC AGTATCTTAC AAGAGTTGTA TTTGACAAAT 
AACAGCCTCA CAGACAAATG TGTGCCCTTG TTAACGGGAC ACCCCCATTT GAAGATCCTT 
CACATGGCCT ATAACCGACT TCAGAGTTTT CCAGCAAGTA AAATGGCGAA ACTGGAGGAA 
CTTGAAGAAA TTGATCTCAG TGGGAATAAG CTGAAAGCCA TCCCAACAAC GATCATGAAT 
TGCAGGCGCA TGCACACCGT GATTGCTCAC TCCAACTGCA TCGAGGTCTT TCCCGAAGTT 
ATGCAGCTCC CAGAGATCAA GTGTGTGGAC CTGAGCTGTA ATGAGCTAAG TGAAGTCACA 
TTACCAGAAA ACCTGCCTCC CAAACTGCAG GAGCTAGACC TGACTGGAAA CCCGCGCCTT 
GTCCTTGATC ACAAAACCCT GGAACTACTG AATAATATCC GCTGTTTCAA GATTGATCAG 
CCTTCTACAG GAGACGCTTC CGGAGCCCCA GCTGTATGGA GTCATGGTTA CACTGAAGCT 
TCGGGGGTAA AAAACAAGTT GTGTGTCGCA GCCCTGTCGG TGAATAACTT CTGTGACAAC 
CGCGAAGCCC TGTATGGTGT GTTTGACGGA GACCGGAATG TGGAGGTGCC CTACCTTCTC 
CAGTGCACTA TGAGTGACAT TTTGGCTGAA GAGCTGCAAA AAACAAAAAA CGAAGAAGAA 
TACATGGTCA ATACATTCAT TGTCATGCAA AGGAAACTTG GAACTGCTGG GCAGAAGCTT 
GGTGGTGCCG CTGTCCTTTG TCATATCAAG CATGACCCTG TGGATCCAGG AGGATCCTTC 
ACCTTGACCT CTGCTAATGT GGGCAAGTGC CAAACAGTTC TCTGTCGAAA TGGAAAGCCG 
CTGCCTCTGT CCAGATCTTA CATCATGAGC TGTGAAGAAG AGCTGAAGAG GATTAAACAG 
CACAAGGCCA TTATCACTGA GGATGGCAAG GTGAACGGAG TGACTGAGTC CACGCGCATC 
CTGGGCTACA CCTTCCTCCA TCCCAGTGTG GTGCCTCGCC CCCACGTGCA GTCCGTGCTC 
CTGACTCCCC AGGATGAGTT CTTCATCCTA GGCAGTAAGG GGTTGTGGGA CAGCCTGTCC 
GTCGAGGAGG CCGTGGAAGC CGTGCGCAAC GTGCCCGATG CCCTGGCTGC TGCCAAGAAG 
CTGTGTACCC TGGCCCAGAG CTACGGCTGC CACGACAGCA TCAGCGCTGT GGTGGTGCAG 
CTCAGTGTCA CTGAGGACAG CTTCTGCTGC TGCGAGCTCA GCGCCGGTGG GGCTGTGCCA 
CCACCCAGTC CTGGCATCTT TCCTCCCTCA GTGAACATGG TGATCAAGGA TCGGCCCTCA 
GATGGGCTGG GCGTGCCGTC CTCCAGCAGC GGCATGGCTT CCGAGATTAG CAGTGAGCTC 
TCCACTTCTG AGATGAGCAG CGAGGTGGGG TCAACAGCCT CCGATGAGCC CCCGCCCGGA 
GCCCTAAGCG AGAACAGCCC TGCCTACCCC AGTGAGCAGC GCTGCATGCT CCACCCCATC 
TGTCTGTCCA ACTCCTTCCA GCGCCAGCTA TCCAGCGCCA CGTTCTCTAG CGCCTTCTCC 
GACAACGGCC TTGACAGTGA CGATGAGGAG CCCATCGAGG GCGTCTTCAC CAACGGCAGC 
CGGGTGGAGG TGGAGGTGGA CATCCACTGC AGCCGGGCCA AGGAGAAGGA GAAACAGCAG 
CACCTGCTTC AGGTGCCAGC AGAGGCCAGT GATGAGGGCA TTGTCATCAG CGCCAACGAG 
GATGAGCCAG GTCTGCCCAG GAAGGCAGAC TTCTCTGCCG TTGGGACCAT TGGGCGCCGG 
AGGGCCAATG GCTCTGTTGC GCCCCAGGAA AGGAGCCACA ATGTGATAGA GGTGGCTACA 
GACGCACCTC TTCGAAAGCC TGGAGGCTAT TTTGCTGCCC CGGCTCAGCC GGATCCTGAT 
GATCAGTTTA TCATACCCCC GGAGCTGGAA GAGGAGGTCA AAGAAATCAT GAAGCATCAC 
CAGGAGCAAC AGCAGCAGCA GCAGCCGCCA CCACCCCCTC AGCTCCAGCC GCAGCTGCCG 
CGGCACTACC AGCTGGACCA GCTGCCAGAT TATTACGACA CGCCACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLPP1 ( NM_194449.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_919431.2 Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mRNA.

Price & Availability↑

CloneID	OHu21282
Clone ID Related Accession (Same CDS sequence)	NM_194449.3 , NM_194449.4
Accession Version	NM_194449.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022046.11, BC126277.1, AC015989.11 and AC027553.6. This sequence is a reference standard in the RefSeqGene project. On Dec 15, 2011 this sequence version replaced NM_194449.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available conservation data and publications. There are no publicly available human transcripts that include the extended region. However, the update is supported by homologous transcript data and is consistent with the full-length 190 kDa human isoform described in the literature. This 190 kDa product, known as PHLPP1beta, has been detected in several studies, including PMIDs 17386267, 19079341, 20089132, 20819118 and 20861921. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011178.2, SRR1803616.65238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCG CCGCCGCGGC CACGGTACAG CGACTCCCCG AGCTCGGCAG GGAGGACCGA 
GCTTCGGCTC CGGCGGCCGC CGCTGCGGCA GCAGCAGCAG CAGCGGCGGC CGCGGCGGCT 
CTGGCGGCGG CGGCCGGGGG CGGCCGGAGT CCGGAGCCCG CGCTGACCCC GGCGGCCCCG 
AGCGGCGGGA ACGGCAGCGG CAGCGGGGCG CGGGAAGAGG CCCCAGGCGA GGCGCCGCCG 
GGGCCGCTGC CGGGCAGAGC GGGGGGTGCC GGGCGCAGGA GGCGGCGCGG GGCGCCCCAG 
CCCATTGCCG GCGGGGCTGC CCCCGTACCC GGGGCCGGCG GCGGCGCCAA CTCCCTCCTG 
CTGAGGAGAG GGCGGCTGAA GAGGAATCTG TCCGCGGCCG CCGCGGCCGC CTCCTCGTCG 
TCGTCGTCCT CGGCCGCTGC TGCCTCGCAC TCCCCCGGCG CTGCCGGCCT CCCCGCCTCC 
TGCTCGGCCT CGGCGTCGCT GTGCACCCGG AGCCTGGACA GGAAGACGCT GCTTCTGAAG 
CACCGGCAGA CGCTGCAGCT GCAGCCGTCG GACCGGGACT GGGTGAGGCA CCAGCTCCAG 
CGCGGCTGCG TGCACGTCTT CGACCGCCAC ATGGCCTCGA CCTACCTGCG CCCGGTGCTC 
TGCACACTGG ACACCACGGC CGGCGAGGTG GCCGCCCGCC TGCTGCAGCT GGGCCACAAA 
GGCGGCGGCG TGGTGAAGGT GCTGGGCCAG GGGCCCGGAG CCGCCGCCGC CCGGGAGCCC 
GCTGAACCGC CCCCCGAGGC CGGCCCCCGG CTGGCGCCCC CGGAGCCGCG GGACTCGGAG 
GTACCGCCCG CGAGGAGCGC GCCGGGTGCC TTCGGGGGGC CTCCGCGCGC GCCCCCCGCC 
GACCTACCCC TGCCCGTCGG CGGCCCGGGC GGGTGGTCGC GCCGCGCCAG CCCAGCGCCC 
TCGGACTCCA GCCCCGGCGA GCCGTTCGTT GGGGGCCCTG TCTCTTCGCC CCGCGCCCCA 
CGGCCTGTGG TCTCCGACAC CGAGAGCTTC AGTCTGAGTC CCAGCGCCGA GAGCGTGTCT 
GACCGGTTGG ACCCCTACAG CAGCGGCGGC GGCTCCTCGT CGTCGTCGGA AGAGCTCGAG 
GCCGACGCAG CCTCGGCCCC GACGGGGGTC CCGGGCCAGC CCCGCCGTCC CGGCCACCCC 
GCGCAGCCCC TCCCGCTTCC CCAGACGGCT TCCTCGCCTC AGCCGCAGCA GAAAGCCCCG 
AGGGCCATTG ACAGCCCGGG CGGGGCCGTC CGCGAGGGGT CGTGCGAGGA GAAGGCAGCG 
GCAGCCGTGG CCCCGGGAGG CCTCCAGTCT ACCCCCGGGA GGAGCGGGGT GACCGCGGAG 
AAGGCGCCTC CGCCGCCCCC GCCGCCCACC CTGTACGTGC AGCTCCACGG AGAGACCACC 
CGGCGCTTGG AGGCGGAGGA GAAGCCATTG CAGATCCAAA ATGACTACCT CTTCCAACTG 
GGATTTGGGG AGCTGTGGAG GGTGCAGGAG GAAGGCATGG ACTCGGAGAT TGGCTGCCTC 
ATCCGCTTCT ATGCAGGAAA ACCTCACAGC ACGGGTAGCT CTGAACGGAT TCAGCTCTCA 
GGAATGTATA ATGTCCGTAA AGGCAAGATG CAGTTGCCAG TGAACCGATG GACAAGACGC 
CAAGTCATCC TATGTGGGAC CTGCCTGATA GTATCATCTG TGAAAGACAG CTTGACCGGA 
AAGATGCATG TTCTGCCACT AATTGGTGGA AAAGTAGAAG AAGTGAAAAA GCACCAACAC 
TGTTTAGCAT TTAGCTCCTC TGGACCCCAA AGCCAGACTT ACTACATTTG CTTTGATACT 
TTCACAGAAT ACTTAAGGTG GCTGCGACAA GTCTCCAAGG TTGCATCCCA GCGCATTAGC 
TCAGTGGACC TCTCGTGTTG TAGCCTGGAA CATCTGCCTG CCAACCTCTT CTACAGCCAA 
GACCTCACTC ATCTCAATTT AAAACAAAAC TTCCTAAGGC AGAACCCTAG CCTTCCAGCT 
GCCAGGGGGC TTAATGAACT GCAAAGGTTC ACCAAGTTGA AGAGTCTTAA CCTTTCCAAT 
AATCATTTAG GGGACTTCCC ACTGGCAGTC TGCAGTATTC CAACCCTGGC AGAGCTGAAC 
GTGTCCTGCA ATGCCCTGCG ATCAGTCCCG GCAGCCGTTG GAGTGATGCA CAACTTACAG 
ACATTTTTGT TGGATGGAAA CTTTCTCCAA TCCCTTCCTG CTGAGTTGGA GAACATGAAG 
CAGCTTAGTT ATCTGGGTCT TTCTTTCAAT GAATTTACTG ACATTCCCGA AGTATTGGAG 
AAATTGACTG CTGTGGATAA ACTTTGTATG TCTGGAAACT GTGTGGAGAC CCTTAGGCTA 
CAGGCTTTAA GAAAAATGCC TCACATTAAA CATGTGGATC TAAGGTTGAA CGTAATTAGG 
AAGCTGATAG CAGATGAAGT GGACTTTCTA CAGCATGTTA CTCAGCTTGA CCTACGAGAC 
AATAAGCTTG GTGATCTAGA TGCTATGATT TTCAACAACA TTGAAGTTTT ACACTGTGAA 
AGGAATCAAC TGGTCACATT AGACATCTGT GGCTATTTCC TAAAAGCGCT CTATGCCTCT 
TCTAATGAAC TTGTTCAACT TGATGTTTAC CCAGTTCCAA ATTATCTGTC CTACATGGAT 
GTTTCAAGGA ACCGCTTAGA AAATGTGCCT GAGTGGGTAT GTGAAAGCCG AAAGCTAGAA 
GTTTTGGATA TTGGCCATAA TCAAATATGT GAACTTCCTG CCCGCTTATT TTGTAATAGC 
AGTCTCCGGA AACTACTGGC AGGACACAAC CAGTTGGCAA GGCTGCCTGA AAGGCTAGAA 
AGAACCTCGG TGGAGGTCTT GGATGTGCAA CACAACCAGC TCCTTGAGCT CCCACCTAAC 
CTTCTGATGA AGGCTGACAG CCTGAGATTC CTGAACGCCT CTGCGAACAA ACTGGAAAGC 
CTTCCTCCAG CCACGCTTTC CGAAGAGACA AACAGTATCT TACAAGAGTT GTATTTGACA 
AATAACAGCC TCACAGACAA ATGTGTGCCC TTGTTAACGG GACACCCCCA TTTGAAGATC 
CTTCACATGG CCTATAACCG ACTTCAGAGT TTTCCAGCAA GTAAAATGGC GAAACTGGAG 
GAACTTGAAG AAATTGATCT CAGTGGGAAT AAGCTGAAAG CCATCCCAAC AACGATCATG 
AATTGCAGGC GCATGCACAC CGTGATTGCT CACTCCAACT GCATCGAGGT CTTTCCCGAA 
GTTATGCAGC TCCCAGAGAT CAAGTGTGTG GACCTGAGCT GTAATGAGCT AAGTGAAGTC 
ACATTACCAG AAAACCTGCC TCCCAAACTG CAGGAGCTAG ACCTGACTGG AAACCCGCGC 
CTTGTCCTTG ATCACAAAAC CCTGGAACTA CTGAATAATA TCCGCTGTTT CAAGATTGAT 
CAGCCTTCTA CAGGAGACGC TTCCGGAGCC CCAGCTGTAT GGAGTCATGG TTACACTGAA 
GCTTCGGGGG TAAAAAACAA GTTGTGTGTC GCAGCCCTGT CGGTGAATAA CTTCTGTGAC 
AACCGCGAAG CCCTGTATGG TGTGTTTGAC GGAGACCGGA ATGTGGAGGT GCCCTACCTT 
CTCCAGTGCA CTATGAGTGA CATTTTGGCT GAAGAGCTGC AAAAAACAAA AAACGAAGAA 
GAATACATGG TCAATACATT CATTGTCATG CAAAGGAAAC TTGGAACTGC TGGGCAGAAG 
CTTGGTGGTG CCGCTGTCCT TTGTCATATC AAGCATGACC CTGTGGATCC AGGAGGATCC 
TTCACCTTGA CCTCTGCTAA TGTGGGCAAG TGCCAAACAG TTCTCTGTCG AAATGGAAAG 
CCGCTGCCTC TGTCCAGATC TTACATCATG AGCTGTGAAG AAGAGCTGAA GAGGATTAAA 
CAGCACAAGG CCATTATCAC TGAGGATGGC AAGGTGAACG GAGTGACTGA GTCCACGCGC 
ATCCTGGGCT ACACCTTCCT CCATCCCAGT GTGGTGCCTC GCCCCCACGT GCAGTCCGTG 
CTCCTGACTC CCCAGGATGA GTTCTTCATC CTAGGCAGTA AGGGGTTGTG GGACAGCCTG 
TCCGTCGAGG AGGCCGTGGA AGCCGTGCGC AACGTGCCCG ATGCCCTGGC TGCTGCCAAG 
AAGCTGTGTA CCCTGGCCCA GAGCTACGGC TGCCACGACA GCATCAGCGC TGTGGTGGTG 
CAGCTCAGTG TCACTGAGGA CAGCTTCTGC TGCTGCGAGC TCAGCGCCGG TGGGGCTGTG 
CCACCACCCA GTCCTGGCAT CTTTCCTCCC TCAGTGAACA TGGTGATCAA GGATCGGCCC 
TCAGATGGGC TGGGCGTGCC GTCCTCCAGC AGCGGCATGG CTTCCGAGAT TAGCAGTGAG 
CTCTCCACTT CTGAGATGAG CAGCGAGGTG GGGTCAACAG CCTCCGATGA GCCCCCGCCC 
GGAGCCCTAA GCGAGAACAG CCCTGCCTAC CCCAGTGAGC AGCGCTGCAT GCTCCACCCC 
ATCTGTCTGT CCAACTCCTT CCAGCGCCAG CTATCCAGCG CCACGTTCTC TAGCGCCTTC 
TCCGACAACG GCCTTGACAG TGACGATGAG GAGCCCATCG AGGGCGTCTT CACCAACGGC 
AGCCGGGTGG AGGTGGAGGT GGACATCCAC TGCAGCCGGG CCAAGGAGAA GGAGAAACAG 
CAGCACCTGC TTCAGGTGCC AGCAGAGGCC AGTGATGAGG GCATTGTCAT CAGCGCCAAC 
GAGGATGAGC CAGGTCTGCC CAGGAAGGCA GACTTCTCTG CCGTTGGGAC CATTGGGCGC 
CGGAGGGCCA ATGGCTCTGT TGCGCCCCAG GAAAGGAGCC ACAATGTGAT AGAGGTGGCT 
ACAGACGCAC CTCTTCGAAA GCCTGGAGGC TATTTTGCTG CCCCGGCTCA GCCGGATCCT 
GATGATCAGT TTATCATACC CCCGGAGCTG GAAGAGGAGG TCAAAGAAAT CATGAAGCAT 
CACCAGGAGC AACAGCAGCA GCAGCAGCCG CCACCACCCC CTCAGCTCCA GCCGCAGCTG 
CCGCGGCACT ACCAGCTGGA CCAGCTGCCA GATTATTACG ACACGCCACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919431.2
CDS	246..5399
Translation	MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL

Target ORF information:

RefSeq Version	NM_194449.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194449.3

ORF Insert Sequence:

ATGGAGCCCG CCGCCGCGGC CACGGTACAG CGACTCCCCG AGCTCGGCAG GGAGGACCGA 
GCTTCGGCTC CGGCGGCCGC CGCTGCGGCA GCAGCAGCAG CAGCGGCGGC CGCGGCGGCT 
CTGGCGGCGG CGGCCGGGGG CGGCCGGAGT CCGGAGCCCG CGCTGACCCC GGCGGCCCCG 
AGCGGCGGGA ACGGCAGCGG CAGCGGGGCG CGGGAAGAGG CCCCAGGCGA GGCGCCGCCG 
GGGCCGCTGC CGGGCAGAGC GGGGGGTGCC GGGCGCAGGA GGCGGCGCGG GGCGCCCCAG 
CCCATTGCCG GCGGGGCTGC CCCCGTACCC GGGGCCGGCG GCGGCGCCAA CTCCCTCCTG 
CTGAGGAGAG GGCGGCTGAA GAGGAATCTG TCCGCGGCCG CCGCGGCCGC CTCCTCGTCG 
TCGTCGTCCT CGGCCGCTGC TGCCTCGCAC TCCCCCGGCG CTGCCGGCCT CCCCGCCTCC 
TGCTCGGCCT CGGCGTCGCT GTGCACCCGG AGCCTGGACA GGAAGACGCT GCTTCTGAAG 
CACCGGCAGA CGCTGCAGCT GCAGCCGTCG GACCGGGACT GGGTGAGGCA CCAGCTCCAG 
CGCGGCTGCG TGCACGTCTT CGACCGCCAC ATGGCCTCGA CCTACCTGCG CCCGGTGCTC 
TGCACACTGG ACACCACGGC CGGCGAGGTG GCCGCCCGCC TGCTGCAGCT GGGCCACAAA 
GGCGGCGGCG TGGTGAAGGT GCTGGGCCAG GGGCCCGGAG CCGCCGCCGC CCGGGAGCCC 
GCTGAACCGC CCCCCGAGGC CGGCCCCCGG CTGGCGCCCC CGGAGCCGCG GGACTCGGAG 
GTACCGCCCG CGAGGAGCGC GCCGGGTGCC TTCGGGGGGC CTCCGCGCGC GCCCCCCGCC 
GACCTACCCC TGCCCGTCGG CGGCCCGGGC GGGTGGTCGC GCCGCGCCAG CCCAGCGCCC 
TCGGACTCCA GCCCCGGCGA GCCGTTCGTT GGGGGCCCTG TCTCTTCGCC CCGCGCCCCA 
CGGCCTGTGG TCTCCGACAC CGAGAGCTTC AGTCTGAGTC CCAGCGCCGA GAGCGTGTCT 
GACCGGTTGG ACCCCTACAG CAGCGGCGGC GGCTCCTCGT CGTCGTCGGA AGAGCTCGAG 
GCCGACGCAG CCTCGGCCCC GACGGGGGTC CCGGGCCAGC CCCGCCGTCC CGGCCACCCC 
GCGCAGCCCC TCCCGCTTCC CCAGACGGCT TCCTCGCCTC AGCCGCAGCA GAAAGCCCCG 
AGGGCCATTG ACAGCCCGGG CGGGGCCGTC CGCGAGGGGT CGTGCGAGGA GAAGGCAGCG 
GCAGCCGTGG CCCCGGGAGG CCTCCAGTCT ACCCCCGGGA GGAGCGGGGT GACCGCGGAG 
AAGGCGCCTC CGCCGCCCCC GCCGCCCACC CTGTACGTGC AGCTCCACGG AGAGACCACC 
CGGCGCTTGG AGGCGGAGGA GAAGCCATTG CAGATCCAAA ATGACTACCT CTTCCAACTG 
GGATTTGGGG AGCTGTGGAG GGTGCAGGAG GAAGGCATGG ACTCGGAGAT TGGCTGCCTC 
ATCCGCTTCT ATGCAGGAAA ACCTCACAGC ACGGGTAGCT CTGAACGGAT TCAGCTCTCA 
GGAATGTATA ATGTCCGTAA AGGCAAGATG CAGTTGCCAG TGAACCGATG GACAAGACGC 
CAAGTCATCC TATGTGGGAC CTGCCTGATA GTATCATCTG TGAAAGACAG CTTGACCGGA 
AAGATGCATG TTCTGCCACT AATTGGTGGA AAAGTAGAAG AAGTGAAAAA GCACCAACAC 
TGTTTAGCAT TTAGCTCCTC TGGACCCCAA AGCCAGACTT ACTACATTTG CTTTGATACT 
TTCACAGAAT ACTTAAGGTG GCTGCGACAA GTCTCCAAGG TTGCATCCCA GCGCATTAGC 
TCAGTGGACC TCTCGTGTTG TAGCCTGGAA CATCTGCCTG CCAACCTCTT CTACAGCCAA 
GACCTCACTC ATCTCAATTT AAAACAAAAC TTCCTAAGGC AGAACCCTAG CCTTCCAGCT 
GCCAGGGGGC TTAATGAACT GCAAAGGTTC ACCAAGTTGA AGAGTCTTAA CCTTTCCAAT 
AATCATTTAG GGGACTTCCC ACTGGCAGTC TGCAGTATTC CAACCCTGGC AGAGCTGAAC 
GTGTCCTGCA ATGCCCTGCG ATCAGTCCCG GCAGCCGTTG GAGTGATGCA CAACTTACAG 
ACATTTTTGT TGGATGGAAA CTTTCTCCAA TCCCTTCCTG CTGAGTTGGA GAACATGAAG 
CAGCTTAGTT ATCTGGGTCT TTCTTTCAAT GAATTTACTG ACATTCCCGA AGTATTGGAG 
AAATTGACTG CTGTGGATAA ACTTTGTATG TCTGGAAACT GTGTGGAGAC CCTTAGGCTA 
CAGGCTTTAA GAAAAATGCC TCACATTAAA CATGTGGATC TAAGGTTGAA CGTAATTAGG 
AAGCTGATAG CAGATGAAGT GGACTTTCTA CAGCATGTTA CTCAGCTTGA CCTACGAGAC 
AATAAGCTTG GTGATCTAGA TGCTATGATT TTCAACAACA TTGAAGTTTT ACACTGTGAA 
AGGAATCAAC TGGTCACATT AGACATCTGT GGCTATTTCC TAAAAGCGCT CTATGCCTCT 
TCTAATGAAC TTGTTCAACT TGATGTTTAC CCAGTTCCAA ATTATCTGTC CTACATGGAT 
GTTTCAAGGA ACCGCTTAGA AAATGTGCCT GAGTGGGTAT GTGAAAGCCG AAAGCTAGAA 
GTTTTGGATA TTGGCCATAA TCAAATATGT GAACTTCCTG CCCGCTTATT TTGTAATAGC 
AGTCTCCGGA AACTACTGGC AGGACACAAC CAGTTGGCAA GGCTGCCTGA AAGGCTAGAA 
AGAACCTCGG TGGAGGTCTT GGATGTGCAA CACAACCAGC TCCTTGAGCT CCCACCTAAC 
CTTCTGATGA AGGCTGACAG CCTGAGATTC CTGAACGCCT CTGCGAACAA ACTGGAAAGC 
CTTCCTCCAG CCACGCTTTC CGAAGAGACA AACAGTATCT TACAAGAGTT GTATTTGACA 
AATAACAGCC TCACAGACAA ATGTGTGCCC TTGTTAACGG GACACCCCCA TTTGAAGATC 
CTTCACATGG CCTATAACCG ACTTCAGAGT TTTCCAGCAA GTAAAATGGC GAAACTGGAG 
GAACTTGAAG AAATTGATCT CAGTGGGAAT AAGCTGAAAG CCATCCCAAC AACGATCATG 
AATTGCAGGC GCATGCACAC CGTGATTGCT CACTCCAACT GCATCGAGGT CTTTCCCGAA 
GTTATGCAGC TCCCAGAGAT CAAGTGTGTG GACCTGAGCT GTAATGAGCT AAGTGAAGTC 
ACATTACCAG AAAACCTGCC TCCCAAACTG CAGGAGCTAG ACCTGACTGG AAACCCGCGC 
CTTGTCCTTG ATCACAAAAC CCTGGAACTA CTGAATAATA TCCGCTGTTT CAAGATTGAT 
CAGCCTTCTA CAGGAGACGC TTCCGGAGCC CCAGCTGTAT GGAGTCATGG TTACACTGAA 
GCTTCGGGGG TAAAAAACAA GTTGTGTGTC GCAGCCCTGT CGGTGAATAA CTTCTGTGAC 
AACCGCGAAG CCCTGTATGG TGTGTTTGAC GGAGACCGGA ATGTGGAGGT GCCCTACCTT 
CTCCAGTGCA CTATGAGTGA CATTTTGGCT GAAGAGCTGC AAAAAACAAA AAACGAAGAA 
GAATACATGG TCAATACATT CATTGTCATG CAAAGGAAAC TTGGAACTGC TGGGCAGAAG 
CTTGGTGGTG CCGCTGTCCT TTGTCATATC AAGCATGACC CTGTGGATCC AGGAGGATCC 
TTCACCTTGA CCTCTGCTAA TGTGGGCAAG TGCCAAACAG TTCTCTGTCG AAATGGAAAG 
CCGCTGCCTC TGTCCAGATC TTACATCATG AGCTGTGAAG AAGAGCTGAA GAGGATTAAA 
CAGCACAAGG CCATTATCAC TGAGGATGGC AAGGTGAACG GAGTGACTGA GTCCACGCGC 
ATCCTGGGCT ACACCTTCCT CCATCCCAGT GTGGTGCCTC GCCCCCACGT GCAGTCCGTG 
CTCCTGACTC CCCAGGATGA GTTCTTCATC CTAGGCAGTA AGGGGTTGTG GGACAGCCTG 
TCCGTCGAGG AGGCCGTGGA AGCCGTGCGC AACGTGCCCG ATGCCCTGGC TGCTGCCAAG 
AAGCTGTGTA CCCTGGCCCA GAGCTACGGC TGCCACGACA GCATCAGCGC TGTGGTGGTG 
CAGCTCAGTG TCACTGAGGA CAGCTTCTGC TGCTGCGAGC TCAGCGCCGG TGGGGCTGTG 
CCACCACCCA GTCCTGGCAT CTTTCCTCCC TCAGTGAACA TGGTGATCAA GGATCGGCCC 
TCAGATGGGC TGGGCGTGCC GTCCTCCAGC AGCGGCATGG CTTCCGAGAT TAGCAGTGAG 
CTCTCCACTT CTGAGATGAG CAGCGAGGTG GGGTCAACAG CCTCCGATGA GCCCCCGCCC 
GGAGCCCTAA GCGAGAACAG CCCTGCCTAC CCCAGTGAGC AGCGCTGCAT GCTCCACCCC 
ATCTGTCTGT CCAACTCCTT CCAGCGCCAG CTATCCAGCG CCACGTTCTC TAGCGCCTTC 
TCCGACAACG GCCTTGACAG TGACGATGAG GAGCCCATCG AGGGCGTCTT CACCAACGGC 
AGCCGGGTGG AGGTGGAGGT GGACATCCAC TGCAGCCGGG CCAAGGAGAA GGAGAAACAG 
CAGCACCTGC TTCAGGTGCC AGCAGAGGCC AGTGATGAGG GCATTGTCAT CAGCGCCAAC 
GAGGATGAGC CAGGTCTGCC CAGGAAGGCA GACTTCTCTG CCGTTGGGAC CATTGGGCGC 
CGGAGGGCCA ATGGCTCTGT TGCGCCCCAG GAAAGGAGCC ACAATGTGAT AGAGGTGGCT 
ACAGACGCAC CTCTTCGAAA GCCTGGAGGC TATTTTGCTG CCCCGGCTCA GCCGGATCCT 
GATGATCAGT TTATCATACC CCCGGAGCTG GAAGAGGAGG TCAAAGAAAT CATGAAGCAT 
CACCAGGAGC AACAGCAGCA GCAGCAGCCG CCACCACCCC CTCAGCTCCA GCCGCAGCTG 
CCGCGGCACT ACCAGCTGGA CCAGCTGCCA GATTATTACG ACACGCCACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLPP1 ( NM_194449.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_919431.2 Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mRNA.

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CloneID	OHu21282
Clone ID Related Accession (Same CDS sequence)	NM_194449.3 , NM_194449.4
Accession Version	NM_194449.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022046.11, BC126277.1, AC015989.11 and AC027553.6. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_194449.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available conservation data and publications. There are no publicly available human transcripts that include the extended region. However, the update is supported by homologous transcript data and is consistent with the full-length 190 kDa human isoform described in the literature. This 190 kDa product, known as PHLPP1beta, has been detected in several studies, including PMIDs 17386267, 19079341, 20089132, 20819118 and 20861921. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014927.2, AB011178.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262719.10/ ENSP00000262719.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCG CCGCCGCGGC CACGGTACAG CGACTCCCCG AGCTCGGCAG GGAGGACCGA 
GCTTCGGCTC CGGCGGCCGC CGCTGCGGCA GCAGCAGCAG CAGCGGCGGC CGCGGCGGCT 
CTGGCGGCGG CGGCCGGGGG CGGCCGGAGT CCGGAGCCCG CGCTGACCCC GGCGGCCCCG 
AGCGGCGGGA ACGGCAGCGG CAGCGGGGCG CGGGAAGAGG CCCCAGGCGA GGCGCCGCCG 
GGGCCGCTGC CGGGCAGAGC GGGGGGTGCC GGGCGCAGGA GGCGGCGCGG GGCGCCCCAG 
CCCATTGCCG GCGGGGCTGC CCCCGTACCC GGGGCCGGCG GCGGCGCCAA CTCCCTCCTG 
CTGAGGAGAG GGCGGCTGAA GAGGAATCTG TCCGCGGCCG CCGCGGCCGC CTCCTCGTCG 
TCGTCGTCCT CGGCCGCTGC TGCCTCGCAC TCCCCCGGCG CTGCCGGCCT CCCCGCCTCC 
TGCTCGGCCT CGGCGTCGCT GTGCACCCGG AGCCTGGACA GGAAGACGCT GCTTCTGAAG 
CACCGGCAGA CGCTGCAGCT GCAGCCGTCG GACCGGGACT GGGTGAGGCA CCAGCTCCAG 
CGCGGCTGCG TGCACGTCTT CGACCGCCAC ATGGCCTCGA CCTACCTGCG CCCGGTGCTC 
TGCACACTGG ACACCACGGC CGGCGAGGTG GCCGCCCGCC TGCTGCAGCT GGGCCACAAA 
GGCGGCGGCG TGGTGAAGGT GCTGGGCCAG GGGCCCGGAG CCGCCGCCGC CCGGGAGCCC 
GCTGAACCGC CCCCCGAGGC CGGCCCCCGG CTGGCGCCCC CGGAGCCGCG GGACTCGGAG 
GTACCGCCCG CGAGGAGCGC GCCGGGTGCC TTCGGGGGGC CTCCGCGCGC GCCCCCCGCC 
GACCTACCCC TGCCCGTCGG CGGCCCGGGC GGGTGGTCGC GCCGCGCCAG CCCAGCGCCC 
TCGGACTCCA GCCCCGGCGA GCCGTTCGTT GGGGGCCCTG TCTCTTCGCC CCGCGCCCCA 
CGGCCTGTGG TCTCCGACAC CGAGAGCTTC AGTCTGAGTC CCAGCGCCGA GAGCGTGTCT 
GACCGGTTGG ACCCCTACAG CAGCGGCGGC GGCTCCTCGT CGTCGTCGGA AGAGCTCGAG 
GCCGACGCAG CCTCGGCCCC GACGGGGGTC CCGGGCCAGC CCCGCCGTCC CGGCCACCCC 
GCGCAGCCCC TCCCGCTTCC CCAGACGGCT TCCTCGCCTC AGCCGCAGCA GAAAGCCCCG 
AGGGCCATTG ACAGCCCGGG CGGGGCCGTC CGCGAGGGGT CGTGCGAGGA GAAGGCAGCG 
GCAGCCGTGG CCCCGGGAGG CCTCCAGTCT ACCCCCGGGA GGAGCGGGGT GACCGCGGAG 
AAGGCGCCTC CGCCGCCCCC GCCGCCCACC CTGTACGTGC AGCTCCACGG AGAGACCACC 
CGGCGCTTGG AGGCGGAGGA GAAGCCATTG CAGATCCAAA ATGACTACCT CTTCCAACTG 
GGATTTGGGG AGCTGTGGAG GGTGCAGGAG GAAGGCATGG ACTCGGAGAT TGGCTGCCTC 
ATCCGCTTCT ATGCAGGAAA ACCTCACAGC ACGGGTAGCT CTGAACGGAT TCAGCTCTCA 
GGAATGTATA ATGTCCGTAA AGGCAAGATG CAGTTGCCAG TGAACCGATG GACAAGACGC 
CAAGTCATCC TATGTGGGAC CTGCCTGATA GTATCATCTG TGAAAGACAG CTTGACCGGA 
AAGATGCATG TTCTGCCACT AATTGGTGGA AAAGTAGAAG AAGTGAAAAA GCACCAACAC 
TGTTTAGCAT TTAGCTCCTC TGGACCCCAA AGCCAGACTT ACTACATTTG CTTTGATACT 
TTCACAGAAT ACTTAAGGTG GCTGCGACAA GTCTCCAAGG TTGCATCCCA GCGCATTAGC 
TCAGTGGACC TCTCGTGTTG TAGCCTGGAA CATCTGCCTG CCAACCTCTT CTACAGCCAA 
GACCTCACTC ATCTCAATTT AAAACAAAAC TTCCTAAGGC AGAACCCTAG CCTTCCAGCT 
GCCAGGGGGC TTAATGAACT GCAAAGGTTC ACCAAGTTGA AGAGTCTTAA CCTTTCCAAT 
AATCATTTAG GGGACTTCCC ACTGGCAGTC TGCAGTATTC CAACCCTGGC AGAGCTGAAC 
GTGTCCTGCA ATGCCCTGCG ATCAGTCCCG GCAGCCGTTG GAGTGATGCA CAACTTACAG 
ACATTTTTGT TGGATGGAAA CTTTCTCCAA TCCCTTCCTG CTGAGTTGGA GAACATGAAG 
CAGCTTAGTT ATCTGGGTCT TTCTTTCAAT GAATTTACTG ACATTCCCGA AGTATTGGAG 
AAATTGACTG CTGTGGATAA ACTTTGTATG TCTGGAAACT GTGTGGAGAC CCTTAGGCTA 
CAGGCTTTAA GAAAAATGCC TCACATTAAA CATGTGGATC TAAGGTTGAA CGTAATTAGG 
AAGCTGATAG CAGATGAAGT GGACTTTCTA CAGCATGTTA CTCAGCTTGA CCTACGAGAC 
AATAAGCTTG GTGATCTAGA TGCTATGATT TTCAACAACA TTGAAGTTTT ACACTGTGAA 
AGGAATCAAC TGGTCACATT AGACATCTGT GGCTATTTCC TAAAAGCGCT CTATGCCTCT 
TCTAATGAAC TTGTTCAACT TGATGTTTAC CCAGTTCCAA ATTATCTGTC CTACATGGAT 
GTTTCAAGGA ACCGCTTAGA AAATGTGCCT GAGTGGGTAT GTGAAAGCCG AAAGCTAGAA 
GTTTTGGATA TTGGCCATAA TCAAATATGT GAACTTCCTG CCCGCTTATT TTGTAATAGC 
AGTCTCCGGA AACTACTGGC AGGACACAAC CAGTTGGCAA GGCTGCCTGA AAGGCTAGAA 
AGAACCTCGG TGGAGGTCTT GGATGTGCAA CACAACCAGC TCCTTGAGCT CCCACCTAAC 
CTTCTGATGA AGGCTGACAG CCTGAGATTC CTGAACGCCT CTGCGAACAA ACTGGAAAGC 
CTTCCTCCAG CCACGCTTTC CGAAGAGACA AACAGTATCT TACAAGAGTT GTATTTGACA 
AATAACAGCC TCACAGACAA ATGTGTGCCC TTGTTAACGG GACACCCCCA TTTGAAGATC 
CTTCACATGG CCTATAACCG ACTTCAGAGT TTTCCAGCAA GTAAAATGGC GAAACTGGAG 
GAACTTGAAG AAATTGATCT CAGTGGGAAT AAGCTGAAAG CCATCCCAAC AACGATCATG 
AATTGCAGGC GCATGCACAC CGTGATTGCT CACTCCAACT GCATCGAGGT CTTTCCCGAA 
GTTATGCAGC TCCCAGAGAT CAAGTGTGTG GACCTGAGCT GTAATGAGCT AAGTGAAGTC 
ACATTACCAG AAAACCTGCC TCCCAAACTG CAGGAGCTAG ACCTGACTGG AAACCCGCGC 
CTTGTCCTTG ATCACAAAAC CCTGGAACTA CTGAATAATA TCCGCTGTTT CAAGATTGAT 
CAGCCTTCTA CAGGAGACGC TTCCGGAGCC CCAGCTGTAT GGAGTCATGG TTACACTGAA 
GCTTCGGGGG TAAAAAACAA GTTGTGTGTC GCAGCCCTGT CGGTGAATAA CTTCTGTGAC 
AACCGCGAAG CCCTGTATGG TGTGTTTGAC GGAGACCGGA ATGTGGAGGT GCCCTACCTT 
CTCCAGTGCA CTATGAGTGA CATTTTGGCT GAAGAGCTGC AAAAAACAAA AAACGAAGAA 
GAATACATGG TCAATACATT CATTGTCATG CAAAGGAAAC TTGGAACTGC TGGGCAGAAG 
CTTGGTGGTG CCGCTGTCCT TTGTCATATC AAGCATGACC CTGTGGATCC AGGAGGATCC 
TTCACCTTGA CCTCTGCTAA TGTGGGCAAG TGCCAAACAG TTCTCTGTCG AAATGGAAAG 
CCGCTGCCTC TGTCCAGATC TTACATCATG AGCTGTGAAG AAGAGCTGAA GAGGATTAAA 
CAGCACAAGG CCATTATCAC TGAGGATGGC AAGGTGAACG GAGTGACTGA GTCCACGCGC 
ATCCTGGGCT ACACCTTCCT CCATCCCAGT GTGGTGCCTC GCCCCCACGT GCAGTCCGTG 
CTCCTGACTC CCCAGGATGA GTTCTTCATC CTAGGCAGTA AGGGGTTGTG GGACAGCCTG 
TCCGTCGAGG AGGCCGTGGA AGCCGTGCGC AACGTGCCCG ATGCCCTGGC TGCTGCCAAG 
AAGCTGTGTA CCCTGGCCCA GAGCTACGGC TGCCACGACA GCATCAGCGC TGTGGTGGTG 
CAGCTCAGTG TCACTGAGGA CAGCTTCTGC TGCTGCGAGC TCAGCGCCGG TGGGGCTGTG 
CCACCACCCA GTCCTGGCAT CTTTCCTCCC TCAGTGAACA TGGTGATCAA GGATCGGCCC 
TCAGATGGGC TGGGCGTGCC GTCCTCCAGC AGCGGCATGG CTTCCGAGAT TAGCAGTGAG 
CTCTCCACTT CTGAGATGAG CAGCGAGGTG GGGTCAACAG CCTCCGATGA GCCCCCGCCC 
GGAGCCCTAA GCGAGAACAG CCCTGCCTAC CCCAGTGAGC AGCGCTGCAT GCTCCACCCC 
ATCTGTCTGT CCAACTCCTT CCAGCGCCAG CTATCCAGCG CCACGTTCTC TAGCGCCTTC 
TCCGACAACG GCCTTGACAG TGACGATGAG GAGCCCATCG AGGGCGTCTT CACCAACGGC 
AGCCGGGTGG AGGTGGAGGT GGACATCCAC TGCAGCCGGG CCAAGGAGAA GGAGAAACAG 
CAGCACCTGC TTCAGGTGCC AGCAGAGGCC AGTGATGAGG GCATTGTCAT CAGCGCCAAC 
GAGGATGAGC CAGGTCTGCC CAGGAAGGCA GACTTCTCTG CCGTTGGGAC CATTGGGCGC 
CGGAGGGCCA ATGGCTCTGT TGCGCCCCAG GAAAGGAGCC ACAATGTGAT AGAGGTGGCT 
ACAGACGCAC CTCTTCGAAA GCCTGGAGGC TATTTTGCTG CCCCGGCTCA GCCGGATCCT 
GATGATCAGT TTATCATACC CCCGGAGCTG GAAGAGGAGG TCAAAGAAAT CATGAAGCAT 
CACCAGGAGC AACAGCAGCA GCAGCAGCCG CCACCACCCC CTCAGCTCCA GCCGCAGCTG 
CCGCGGCACT ACCAGCTGGA CCAGCTGCCA GATTATTACG ACACGCCACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919431.2
CDS	144..5297
Translation	MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL

Target ORF information:

RefSeq Version	NM_194449.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194449.4

ORF Insert Sequence:

ATGGAGCCCG CCGCCGCGGC CACGGTACAG CGACTCCCCG AGCTCGGCAG GGAGGACCGA 
GCTTCGGCTC CGGCGGCCGC CGCTGCGGCA GCAGCAGCAG CAGCGGCGGC CGCGGCGGCT 
CTGGCGGCGG CGGCCGGGGG CGGCCGGAGT CCGGAGCCCG CGCTGACCCC GGCGGCCCCG 
AGCGGCGGGA ACGGCAGCGG CAGCGGGGCG CGGGAAGAGG CCCCAGGCGA GGCGCCGCCG 
GGGCCGCTGC CGGGCAGAGC GGGGGGTGCC GGGCGCAGGA GGCGGCGCGG GGCGCCCCAG 
CCCATTGCCG GCGGGGCTGC CCCCGTACCC GGGGCCGGCG GCGGCGCCAA CTCCCTCCTG 
CTGAGGAGAG GGCGGCTGAA GAGGAATCTG TCCGCGGCCG CCGCGGCCGC CTCCTCGTCG 
TCGTCGTCCT CGGCCGCTGC TGCCTCGCAC TCCCCCGGCG CTGCCGGCCT CCCCGCCTCC 
TGCTCGGCCT CGGCGTCGCT GTGCACCCGG AGCCTGGACA GGAAGACGCT GCTTCTGAAG 
CACCGGCAGA CGCTGCAGCT GCAGCCGTCG GACCGGGACT GGGTGAGGCA CCAGCTCCAG 
CGCGGCTGCG TGCACGTCTT CGACCGCCAC ATGGCCTCGA CCTACCTGCG CCCGGTGCTC 
TGCACACTGG ACACCACGGC CGGCGAGGTG GCCGCCCGCC TGCTGCAGCT GGGCCACAAA 
GGCGGCGGCG TGGTGAAGGT GCTGGGCCAG GGGCCCGGAG CCGCCGCCGC CCGGGAGCCC 
GCTGAACCGC CCCCCGAGGC CGGCCCCCGG CTGGCGCCCC CGGAGCCGCG GGACTCGGAG 
GTACCGCCCG CGAGGAGCGC GCCGGGTGCC TTCGGGGGGC CTCCGCGCGC GCCCCCCGCC 
GACCTACCCC TGCCCGTCGG CGGCCCGGGC GGGTGGTCGC GCCGCGCCAG CCCAGCGCCC 
TCGGACTCCA GCCCCGGCGA GCCGTTCGTT GGGGGCCCTG TCTCTTCGCC CCGCGCCCCA 
CGGCCTGTGG TCTCCGACAC CGAGAGCTTC AGTCTGAGTC CCAGCGCCGA GAGCGTGTCT 
GACCGGTTGG ACCCCTACAG CAGCGGCGGC GGCTCCTCGT CGTCGTCGGA AGAGCTCGAG 
GCCGACGCAG CCTCGGCCCC GACGGGGGTC CCGGGCCAGC CCCGCCGTCC CGGCCACCCC 
GCGCAGCCCC TCCCGCTTCC CCAGACGGCT TCCTCGCCTC AGCCGCAGCA GAAAGCCCCG 
AGGGCCATTG ACAGCCCGGG CGGGGCCGTC CGCGAGGGGT CGTGCGAGGA GAAGGCAGCG 
GCAGCCGTGG CCCCGGGAGG CCTCCAGTCT ACCCCCGGGA GGAGCGGGGT GACCGCGGAG 
AAGGCGCCTC CGCCGCCCCC GCCGCCCACC CTGTACGTGC AGCTCCACGG AGAGACCACC 
CGGCGCTTGG AGGCGGAGGA GAAGCCATTG CAGATCCAAA ATGACTACCT CTTCCAACTG 
GGATTTGGGG AGCTGTGGAG GGTGCAGGAG GAAGGCATGG ACTCGGAGAT TGGCTGCCTC 
ATCCGCTTCT ATGCAGGAAA ACCTCACAGC ACGGGTAGCT CTGAACGGAT TCAGCTCTCA 
GGAATGTATA ATGTCCGTAA AGGCAAGATG CAGTTGCCAG TGAACCGATG GACAAGACGC 
CAAGTCATCC TATGTGGGAC CTGCCTGATA GTATCATCTG TGAAAGACAG CTTGACCGGA 
AAGATGCATG TTCTGCCACT AATTGGTGGA AAAGTAGAAG AAGTGAAAAA GCACCAACAC 
TGTTTAGCAT TTAGCTCCTC TGGACCCCAA AGCCAGACTT ACTACATTTG CTTTGATACT 
TTCACAGAAT ACTTAAGGTG GCTGCGACAA GTCTCCAAGG TTGCATCCCA GCGCATTAGC 
TCAGTGGACC TCTCGTGTTG TAGCCTGGAA CATCTGCCTG CCAACCTCTT CTACAGCCAA 
GACCTCACTC ATCTCAATTT AAAACAAAAC TTCCTAAGGC AGAACCCTAG CCTTCCAGCT 
GCCAGGGGGC TTAATGAACT GCAAAGGTTC ACCAAGTTGA AGAGTCTTAA CCTTTCCAAT 
AATCATTTAG GGGACTTCCC ACTGGCAGTC TGCAGTATTC CAACCCTGGC AGAGCTGAAC 
GTGTCCTGCA ATGCCCTGCG ATCAGTCCCG GCAGCCGTTG GAGTGATGCA CAACTTACAG 
ACATTTTTGT TGGATGGAAA CTTTCTCCAA TCCCTTCCTG CTGAGTTGGA GAACATGAAG 
CAGCTTAGTT ATCTGGGTCT TTCTTTCAAT GAATTTACTG ACATTCCCGA AGTATTGGAG 
AAATTGACTG CTGTGGATAA ACTTTGTATG TCTGGAAACT GTGTGGAGAC CCTTAGGCTA 
CAGGCTTTAA GAAAAATGCC TCACATTAAA CATGTGGATC TAAGGTTGAA CGTAATTAGG 
AAGCTGATAG CAGATGAAGT GGACTTTCTA CAGCATGTTA CTCAGCTTGA CCTACGAGAC 
AATAAGCTTG GTGATCTAGA TGCTATGATT TTCAACAACA TTGAAGTTTT ACACTGTGAA 
AGGAATCAAC TGGTCACATT AGACATCTGT GGCTATTTCC TAAAAGCGCT CTATGCCTCT 
TCTAATGAAC TTGTTCAACT TGATGTTTAC CCAGTTCCAA ATTATCTGTC CTACATGGAT 
GTTTCAAGGA ACCGCTTAGA AAATGTGCCT GAGTGGGTAT GTGAAAGCCG AAAGCTAGAA 
GTTTTGGATA TTGGCCATAA TCAAATATGT GAACTTCCTG CCCGCTTATT TTGTAATAGC 
AGTCTCCGGA AACTACTGGC AGGACACAAC CAGTTGGCAA GGCTGCCTGA AAGGCTAGAA 
AGAACCTCGG TGGAGGTCTT GGATGTGCAA CACAACCAGC TCCTTGAGCT CCCACCTAAC 
CTTCTGATGA AGGCTGACAG CCTGAGATTC CTGAACGCCT CTGCGAACAA ACTGGAAAGC 
CTTCCTCCAG CCACGCTTTC CGAAGAGACA AACAGTATCT TACAAGAGTT GTATTTGACA 
AATAACAGCC TCACAGACAA ATGTGTGCCC TTGTTAACGG GACACCCCCA TTTGAAGATC 
CTTCACATGG CCTATAACCG ACTTCAGAGT TTTCCAGCAA GTAAAATGGC GAAACTGGAG 
GAACTTGAAG AAATTGATCT CAGTGGGAAT AAGCTGAAAG CCATCCCAAC AACGATCATG 
AATTGCAGGC GCATGCACAC CGTGATTGCT CACTCCAACT GCATCGAGGT CTTTCCCGAA 
GTTATGCAGC TCCCAGAGAT CAAGTGTGTG GACCTGAGCT GTAATGAGCT AAGTGAAGTC 
ACATTACCAG AAAACCTGCC TCCCAAACTG CAGGAGCTAG ACCTGACTGG AAACCCGCGC 
CTTGTCCTTG ATCACAAAAC CCTGGAACTA CTGAATAATA TCCGCTGTTT CAAGATTGAT 
CAGCCTTCTA CAGGAGACGC TTCCGGAGCC CCAGCTGTAT GGAGTCATGG TTACACTGAA 
GCTTCGGGGG TAAAAAACAA GTTGTGTGTC GCAGCCCTGT CGGTGAATAA CTTCTGTGAC 
AACCGCGAAG CCCTGTATGG TGTGTTTGAC GGAGACCGGA ATGTGGAGGT GCCCTACCTT 
CTCCAGTGCA CTATGAGTGA CATTTTGGCT GAAGAGCTGC AAAAAACAAA AAACGAAGAA 
GAATACATGG TCAATACATT CATTGTCATG CAAAGGAAAC TTGGAACTGC TGGGCAGAAG 
CTTGGTGGTG CCGCTGTCCT TTGTCATATC AAGCATGACC CTGTGGATCC AGGAGGATCC 
TTCACCTTGA CCTCTGCTAA TGTGGGCAAG TGCCAAACAG TTCTCTGTCG AAATGGAAAG 
CCGCTGCCTC TGTCCAGATC TTACATCATG AGCTGTGAAG AAGAGCTGAA GAGGATTAAA 
CAGCACAAGG CCATTATCAC TGAGGATGGC AAGGTGAACG GAGTGACTGA GTCCACGCGC 
ATCCTGGGCT ACACCTTCCT CCATCCCAGT GTGGTGCCTC GCCCCCACGT GCAGTCCGTG 
CTCCTGACTC CCCAGGATGA GTTCTTCATC CTAGGCAGTA AGGGGTTGTG GGACAGCCTG 
TCCGTCGAGG AGGCCGTGGA AGCCGTGCGC AACGTGCCCG ATGCCCTGGC TGCTGCCAAG 
AAGCTGTGTA CCCTGGCCCA GAGCTACGGC TGCCACGACA GCATCAGCGC TGTGGTGGTG 
CAGCTCAGTG TCACTGAGGA CAGCTTCTGC TGCTGCGAGC TCAGCGCCGG TGGGGCTGTG 
CCACCACCCA GTCCTGGCAT CTTTCCTCCC TCAGTGAACA TGGTGATCAA GGATCGGCCC 
TCAGATGGGC TGGGCGTGCC GTCCTCCAGC AGCGGCATGG CTTCCGAGAT TAGCAGTGAG 
CTCTCCACTT CTGAGATGAG CAGCGAGGTG GGGTCAACAG CCTCCGATGA GCCCCCGCCC 
GGAGCCCTAA GCGAGAACAG CCCTGCCTAC CCCAGTGAGC AGCGCTGCAT GCTCCACCCC 
ATCTGTCTGT CCAACTCCTT CCAGCGCCAG CTATCCAGCG CCACGTTCTC TAGCGCCTTC 
TCCGACAACG GCCTTGACAG TGACGATGAG GAGCCCATCG AGGGCGTCTT CACCAACGGC 
AGCCGGGTGG AGGTGGAGGT GGACATCCAC TGCAGCCGGG CCAAGGAGAA GGAGAAACAG 
CAGCACCTGC TTCAGGTGCC AGCAGAGGCC AGTGATGAGG GCATTGTCAT CAGCGCCAAC 
GAGGATGAGC CAGGTCTGCC CAGGAAGGCA GACTTCTCTG CCGTTGGGAC CATTGGGCGC 
CGGAGGGCCA ATGGCTCTGT TGCGCCCCAG GAAAGGAGCC ACAATGTGAT AGAGGTGGCT 
ACAGACGCAC CTCTTCGAAA GCCTGGAGGC TATTTTGCTG CCCCGGCTCA GCCGGATCCT 
GATGATCAGT TTATCATACC CCCGGAGCTG GAAGAGGAGG TCAAAGAAAT CATGAAGCAT 
CACCAGGAGC AACAGCAGCA GCAGCAGCCG CCACCACCCC CTCAGCTCCA GCCGCAGCTG 
CCGCGGCACT ACCAGCTGGA CCAGCTGCCA GATTATTACG ACACGCCACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLPP1 ( XM_011525886.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524188.1 Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), transcript variant X2, mRNA.

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CloneID	OHu65770
Clone ID Related Accession (Same CDS sequence)	XM_011525886.1
Accession Version	XM_011525886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PH domain leucine-rich repeat-containing protein phosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGCCGCGGC CACGGTACAG CGACTCCCCG AGCTCGGCAG GGAGGACCGA 
GCTTCGGCTC CGGCGGCCGC CGCTGCGGCA GCAGCAGCAG CAGCGGCGGC CGCGGCGGCT 
CTGGCGGCGG CGGCCGGGGG CGGCCGGAGT CCGGAGCCCG CGCTGACCCC GGCGGCCCCG 
AGCGGCGGGA ACGGCAGCGG CAGCGGGGCG CGGGAAGAGG CCCCAGGCGA GGCGCCGCCG 
GGGCCGCTGC CGGGCAGAGC GGGGGGTGCC GGGCGCAGGA GGCGGCGCGG GGCGCCCCAG 
CCCATTGCCG GCGGGGCTGC CCCCGTACCC GGGGCCGGCG GCGGCGCCAA CTCCCTCCTG 
CTGAGGAGAG GGCGGCTGAA GAGGAATCTG TCCGCGGCCG CCGCGGCCGC CTCCTCGTCG 
TCGTCGTCCT CGGCCGCTGC TGCCTCGCAC TCCCCCGGCG CTGCCGGCCT CCCCGCCTCC 
TGCTCGGCCT CGGCGTCGCT GTGCACCCGG AGCCTGGACA GGAAGACGCT GCTTCTGAAG 
CACCGGCAGA CGCTGCAGCT GCAGCCGTCG GACCGGGACT GGGTGAGGCA CCAGCTCCAG 
CGCGGCTGCG TGCACGTCTT CGACCGCCAC ATGGCCTCGA CCTACCTGCG CCCGGTGCTC 
TGCACACTGG ACACCACGGC CGGCGAGGTG GCCGCCCGCC TGCTGCAGCT GGGCCACAAA 
GGCGGCGGCG TGGTGAAGGT GCTGGGCCAG GGGCCCGGAG CCGCCGCCGC CCGGGAGCCC 
GCTGAACCGC CCCCCGAGGC CGGCCCCCGG CTGGCGCCCC CGGAGCCGCG GGACTCGGAG 
GTACCGCCCG CGAGGAGCGC GCCGGGTGCC TTCGGGGGGC CTCCGCGCGC GCCCCCCGCC 
GACCTACCCC TGCCCGTCGG CGGCCCGGGC GGGTGGTCGC GCCGCGCCAG CCCAGCGCCC 
TCGGACTCCA GCCCCGGCGA GCCGTTCGTT GGGGGCCCTG TCTCTTCGCC CCGCGCCCCA 
CGGCCTGTGG TCTCCGACAC CGAGAGCTTC AGTCTGAGTC CCAGCGCCGA GAGCGTGTCT 
GACCGGTTGG ACCCCTACAG CAGCGGCGGC GGCTCCTCGT CGTCGTCGGA AGAGCTCGAG 
GCCGACGCAG CCTCGGCCCC GACGGGGGTC CCGGGCCAGC CCCGCCGTCC CGGCCACCCC 
GCGCAGCCCC TCCCGCTTCC CCAGACGGCT TCCTCGCCTC AGCCGCAGCA GAAAGCCCCG 
AGGGCCATTG ACAGCCCGGG CGGGGCCGTC CGCGAGGGGT CGTGCGAGGA GAAGGCAGCG 
GCAGCCGTGG CCCCGGGAGG CCTCCAGTCT ACCCCCGGGA GGAGCGGGGT GACCGCGGAG 
AAGGCGCCTC CGCCGCCCCC GCCGCCCACC CTGTACGTGC AGCTCCACGG AGAGACCACC 
CGGCGCTTGG AGGCGGAGGA GAAGCCATTG CAGATCCAAA ATGACTACCT CTTCCAACTG 
GGATTTGGGG AGCTGTGGAG GGTGCAGGAG GAAGGCATGG ACTCGGAGAT TGGCTGCCTC 
ATCCGCTTCT ATGCAGGAAA ACCTCACAGC ACGGGTAGCT CTGAACGGAT TCAGCTCTCA 
GGAATGTATA ATGTCCGTAA AGGCAAGATG CAGTTGCCAG TGAACCGATG GACAAGACGC 
CAAGTCATCC TATGTGGGAC CTGCCTGATA GTATCATCTG TGAAAGACAG CTTGACCGGA 
AAGATGCATG TTCTGCCACT AATTGGTGGA AAAGTAGAAG AAGTGAAAAA GCACCAACAC 
TGTTTAGCAT TTAGCTCCTC TGGACCCCAA AGCCAGACTT ACTACATTTG CTTTGATACT 
TTCACAGAAT ACTTAAGGTG GCTGCGACAA GTCTCCAAGG TTGCATCCCA GCGCATTAGC 
TCAGTGGACC TCTCGTGTTG TAGCCTGGAA CATCTGCCTG CCAACCTCTT CTACAGCCAA 
GACCTCACTC ATCTCAATTT AAAACAAAAC TTCCTAAGGC AGAACCCTAG CCTTCCAGCT 
GCCAGGGGGC TTAATGAACT GCAAAGGTTC ACCAAGTTGA AGAGTCTTAA CCTTTCCAAT 
AATCATTTAG GGGACTTCCC ACTGGCAGTC TGCAGTATTC CAACCCTGGC AGAGCTGAAC 
GTGTCCTGCA ATGCCCTGCG ATCAGTCCCG GCAGCCGTTG GAGTGATGCA CAACTTACAG 
ACATTTTTGT TGGATGGAAA CTTTCTCCAA TCCCTTCCTG CTGAGTTGGA GAACATGAAG 
CAGCTTAGTT ATCTGGGTCT TTCTTTCAAT GAATTTACTG ACATTCCCGA AGTATTGGAG 
AAATTGACTG CTGTGGATAA ACTTTGTATG TCTGGAAACT GTGTGGAGAC CCTTAGGCTA 
CAGGCTTTAA GAAAAATGCC TCACATTAAA CATGTGGATC TAAGGTTGAA CGTAATTAGG 
AAGCTGATAG CAGATGAAGT GGACTTTCTA CAGCATGTTA CTCAGCTTGA CCTACGAGAC 
AATAAGCTTG GTGATCTAGA TGCTATGATT TTCAACAACA TTGAAGTTTT ACACTGTGAA 
AGGAATCAAC TGGTCACATT AGACATCTGT GGCTATTTCC TAAAAGCGCT CTATGCCTCT 
TCTAATGAAC TTGTTCAACT TGATGTTTAC CCAGTTCCAA ATTATCTGTC CTACATGGAT 
GTTTCAAGGA ACCGCTTAGA AAATGTGCCT GAGTGGGTAT GTGAAAGCCG AAAGCTAGAA 
GTTTTGGATA TTGGCCATAA TCAAATATGT GAACTTCCTG CCCGCTTATT TTGTAATAGC 
AGTCTCCGGA AACTACTGGC AGGACACAAC CAGTTGGCAA GGCTGCCTGA AAGGCTAGAA 
AGAACCTCGG TGGAGGTCTT GGATGTGCAA CACAACCAGC TCCTTGAGCT CCCACCTAAC 
CTTCTGATGA AGGCTGACAG CCTGAGATTC CTGAACGCCT CTGCGAACAA ACTGGAAAGC 
CTTCCTCCAG CCACGCTTTC CGAAGAGACA AACAGTATCT TACAAGAGTT GTATTTGACA 
AATAACAGCC TCACAGACAA ATGTGTGCCC TTGTTAACGG GACACCCCCA TTTGAAGATC 
CTTCACATGG CCTATAACCG ACTTCAGAGT TTTCCAGCAA GTAAAATGGC GAAACTGGAG 
GAACTTGAAG AAATTGATCT CAGTGGGAAT AAGCTGAAAG CCATCCCAAC AACGATCATG 
AATTGCAGGC GCATGCACAC CGTGATTGCT CACTCCAACT GCATCGAGGT CTTTCCCGAA 
GTTATGCAGC TCCCAGAGAT CAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524188.1
CDS	240..3569
Translation	MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKD

Target ORF information:

RefSeq Version	XM_011525886.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525886.1

ORF Insert Sequence:

ATGGAGCCCG CCGCCGCGGC CACGGTACAG CGACTCCCCG AGCTCGGCAG GGAGGACCGA 
GCTTCGGCTC CGGCGGCCGC CGCTGCGGCA GCAGCAGCAG CAGCGGCGGC CGCGGCGGCT 
CTGGCGGCGG CGGCCGGGGG CGGCCGGAGT CCGGAGCCCG CGCTGACCCC GGCGGCCCCG 
AGCGGCGGGA ACGGCAGCGG CAGCGGGGCG CGGGAAGAGG CCCCAGGCGA GGCGCCGCCG 
GGGCCGCTGC CGGGCAGAGC GGGGGGTGCC GGGCGCAGGA GGCGGCGCGG GGCGCCCCAG 
CCCATTGCCG GCGGGGCTGC CCCCGTACCC GGGGCCGGCG GCGGCGCCAA CTCCCTCCTG 
CTGAGGAGAG GGCGGCTGAA GAGGAATCTG TCCGCGGCCG CCGCGGCCGC CTCCTCGTCG 
TCGTCGTCCT CGGCCGCTGC TGCCTCGCAC TCCCCCGGCG CTGCCGGCCT CCCCGCCTCC 
TGCTCGGCCT CGGCGTCGCT GTGCACCCGG AGCCTGGACA GGAAGACGCT GCTTCTGAAG 
CACCGGCAGA CGCTGCAGCT GCAGCCGTCG GACCGGGACT GGGTGAGGCA CCAGCTCCAG 
CGCGGCTGCG TGCACGTCTT CGACCGCCAC ATGGCCTCGA CCTACCTGCG CCCGGTGCTC 
TGCACACTGG ACACCACGGC CGGCGAGGTG GCCGCCCGCC TGCTGCAGCT GGGCCACAAA 
GGCGGCGGCG TGGTGAAGGT GCTGGGCCAG GGGCCCGGAG CCGCCGCCGC CCGGGAGCCC 
GCTGAACCGC CCCCCGAGGC CGGCCCCCGG CTGGCGCCCC CGGAGCCGCG GGACTCGGAG 
GTACCGCCCG CGAGGAGCGC GCCGGGTGCC TTCGGGGGGC CTCCGCGCGC GCCCCCCGCC 
GACCTACCCC TGCCCGTCGG CGGCCCGGGC GGGTGGTCGC GCCGCGCCAG CCCAGCGCCC 
TCGGACTCCA GCCCCGGCGA GCCGTTCGTT GGGGGCCCTG TCTCTTCGCC CCGCGCCCCA 
CGGCCTGTGG TCTCCGACAC CGAGAGCTTC AGTCTGAGTC CCAGCGCCGA GAGCGTGTCT 
GACCGGTTGG ACCCCTACAG CAGCGGCGGC GGCTCCTCGT CGTCGTCGGA AGAGCTCGAG 
GCCGACGCAG CCTCGGCCCC GACGGGGGTC CCGGGCCAGC CCCGCCGTCC CGGCCACCCC 
GCGCAGCCCC TCCCGCTTCC CCAGACGGCT TCCTCGCCTC AGCCGCAGCA GAAAGCCCCG 
AGGGCCATTG ACAGCCCGGG CGGGGCCGTC CGCGAGGGGT CGTGCGAGGA GAAGGCAGCG 
GCAGCCGTGG CCCCGGGAGG CCTCCAGTCT ACCCCCGGGA GGAGCGGGGT GACCGCGGAG 
AAGGCGCCTC CGCCGCCCCC GCCGCCCACC CTGTACGTGC AGCTCCACGG AGAGACCACC 
CGGCGCTTGG AGGCGGAGGA GAAGCCATTG CAGATCCAAA ATGACTACCT CTTCCAACTG 
GGATTTGGGG AGCTGTGGAG GGTGCAGGAG GAAGGCATGG ACTCGGAGAT TGGCTGCCTC 
ATCCGCTTCT ATGCAGGAAA ACCTCACAGC ACGGGTAGCT CTGAACGGAT TCAGCTCTCA 
GGAATGTATA ATGTCCGTAA AGGCAAGATG CAGTTGCCAG TGAACCGATG GACAAGACGC 
CAAGTCATCC TATGTGGGAC CTGCCTGATA GTATCATCTG TGAAAGACAG CTTGACCGGA 
AAGATGCATG TTCTGCCACT AATTGGTGGA AAAGTAGAAG AAGTGAAAAA GCACCAACAC 
TGTTTAGCAT TTAGCTCCTC TGGACCCCAA AGCCAGACTT ACTACATTTG CTTTGATACT 
TTCACAGAAT ACTTAAGGTG GCTGCGACAA GTCTCCAAGG TTGCATCCCA GCGCATTAGC 
TCAGTGGACC TCTCGTGTTG TAGCCTGGAA CATCTGCCTG CCAACCTCTT CTACAGCCAA 
GACCTCACTC ATCTCAATTT AAAACAAAAC TTCCTAAGGC AGAACCCTAG CCTTCCAGCT 
GCCAGGGGGC TTAATGAACT GCAAAGGTTC ACCAAGTTGA AGAGTCTTAA CCTTTCCAAT 
AATCATTTAG GGGACTTCCC ACTGGCAGTC TGCAGTATTC CAACCCTGGC AGAGCTGAAC 
GTGTCCTGCA ATGCCCTGCG ATCAGTCCCG GCAGCCGTTG GAGTGATGCA CAACTTACAG 
ACATTTTTGT TGGATGGAAA CTTTCTCCAA TCCCTTCCTG CTGAGTTGGA GAACATGAAG 
CAGCTTAGTT ATCTGGGTCT TTCTTTCAAT GAATTTACTG ACATTCCCGA AGTATTGGAG 
AAATTGACTG CTGTGGATAA ACTTTGTATG TCTGGAAACT GTGTGGAGAC CCTTAGGCTA 
CAGGCTTTAA GAAAAATGCC TCACATTAAA CATGTGGATC TAAGGTTGAA CGTAATTAGG 
AAGCTGATAG CAGATGAAGT GGACTTTCTA CAGCATGTTA CTCAGCTTGA CCTACGAGAC 
AATAAGCTTG GTGATCTAGA TGCTATGATT TTCAACAACA TTGAAGTTTT ACACTGTGAA 
AGGAATCAAC TGGTCACATT AGACATCTGT GGCTATTTCC TAAAAGCGCT CTATGCCTCT 
TCTAATGAAC TTGTTCAACT TGATGTTTAC CCAGTTCCAA ATTATCTGTC CTACATGGAT 
GTTTCAAGGA ACCGCTTAGA AAATGTGCCT GAGTGGGTAT GTGAAAGCCG AAAGCTAGAA 
GTTTTGGATA TTGGCCATAA TCAAATATGT GAACTTCCTG CCCGCTTATT TTGTAATAGC 
AGTCTCCGGA AACTACTGGC AGGACACAAC CAGTTGGCAA GGCTGCCTGA AAGGCTAGAA 
AGAACCTCGG TGGAGGTCTT GGATGTGCAA CACAACCAGC TCCTTGAGCT CCCACCTAAC 
CTTCTGATGA AGGCTGACAG CCTGAGATTC CTGAACGCCT CTGCGAACAA ACTGGAAAGC 
CTTCCTCCAG CCACGCTTTC CGAAGAGACA AACAGTATCT TACAAGAGTT GTATTTGACA 
AATAACAGCC TCACAGACAA ATGTGTGCCC TTGTTAACGG GACACCCCCA TTTGAAGATC 
CTTCACATGG CCTATAACCG ACTTCAGAGT TTTCCAGCAA GTAAAATGGC GAAACTGGAG 
GAACTTGAAG AAATTGATCT CAGTGGGAAT AAGCTGAAAG CCATCCCAAC AACGATCATG 
AATTGCAGGC GCATGCACAC CGTGATTGCT CACTCCAACT GCATCGAGGT CTTTCCCGAA 
GTTATGCAGC TCCCAGAGAT CAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1.
The Journal of biological chemistry292(34)13947-13958(2017 08)
Gangula NR,Maddika S

LPS depletes PHLPP levels in macrophages through the inhibition of SP1 dependent transcriptional regulation.
Biochemical and biophysical research communications486(2)533-538(2017 04)
Alamuru-Yellapragada NP,Vundyala S,Behera S,Parsa KV

Increased levels of the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP)-1 in obese participants are associated with insulin resistance.
Diabetologia54(7)1879-87(2011 Jul)
Andreozzi F,Procopio C,Greco A,Mannino GC,Miele C,Raciti GA,Iadicicco C,Beguinot F,Pontiroli AE,Hribal ML,Folli F,Sesti G

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PHLPP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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